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159 results on '"Bloyet, Louis-Marie"'

1. Endosomal fusion of pH-dependent enveloped viruses requires ion channel TRPM7

Author

Doyle, Catherine A., Busey, Gregory W., Iobst, Wesley H., Kiessling, Volker, Renken, Chloe, Doppalapudi, Hansa, Stremska, Marta E., Manjegowda, Mohan C., Arish, Mohd, Wang, Weiming, Naphade, Shardul, Kennedy, Joel, Bloyet, Louis-Marie, Thompson, Cassandra E., Rothlauf, Paul W., Stipes, Eric J., Whelan, Sean P. J., Tamm, Lukas K., Kreutzberger, Alex J. B., Sun, Jie, and Desai, Bimal N.

Published
2024
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3. SARS-CoV-2 productively infects primary human immune system cells in vitro and in COVID-19 patients

Author

Pontelli, Marjorie C, Castro, Ítalo A, Martins, Ronaldo B, La Serra, Leonardo, Veras, Flávio P, Nascimento, Daniele C, Silva, Camila M, Cardoso, Ricardo S, Rosales, Roberta, Gomes, Rogério, Lima, Thais M, Souza, Juliano P, Vitti, Brenda C, Caetité, Diego B, de Lima, Mikhael HF, Stumpf, Spencer D, Thompson, Cassandra E, Bloyet, Louis-Marie, Toller-Kawahisa, Juliana E, Giannini, Marcela C, Bonjorno, Letícia P, Lopes, Maria IF, Batah, Sabrina S, Siyuan, Li, Luppino-Assad, Rodrigo, Almeida, Sergio CL, Oliveira, Fabiola R, Benatti, Maíra N, Pontes, Lorena LF, Santana, Rodrigo C, Vilar, Fernando C, Auxiliadora-Martins, Maria, Shi, Pei-Yong, Cunha, Thiago M, Calado, Rodrigo T, Alves-Filho, José C, Zamboni, Dario S, Fabro, Alexandre T, Louzada-Junior, Paulo, Oliveira, Rene DR, Whelan, Sean PJ, Cunha, Fernando Q, and Arruda, Eurico

Abstract

Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with a hyperinflammatory state and lymphocytopenia, a hallmark that appears as both signature and prognosis of disease severity outcome. Although cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion, it is still unclear whether direct SARS-CoV-2 infection of immune cells could also play a role in this scenario by harboring viral replication. We found that monocytes, as well as both B and T lymphocytes, were susceptible to SARS-CoV-2 infection in vitro, accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive T cell apoptosis. In addition, flow cytometry and immunofluorescence analysis revealed that SARS-CoV-2 was frequently detected in monocytes and B lymphocytes from coronavirus disease 2019 (COVID-19) patients. The rates of SARS-CoV-2-infected monocytes in peripheral blood mononuclear cells from COVID-19 patients increased over time from symptom onset, with SARS-CoV-2-positive monocytes, B cells, and CD4+ T lymphocytes also detected in postmortem lung tissue. These results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in COVID-19 patients might have important implications for disease pathogenesis and progression, immune dysfunction, and virus spread within the host.

Published
2022

4. Defining the risk of SARS-CoV-2 variants on immune protection

Author

DeGrace, Marciela M, Ghedin, Elodie, Frieman, Matthew B, Krammer, Florian, Grifoni, Alba, Alisoltani, Arghavan, Alter, Galit, Amara, Rama R, Baric, Ralph S, Barouch, Dan H, Bloom, Jesse D, Bloyet, Louis-Marie, Bonenfant, Gaston, Boon, Adrianus CM, Boritz, Eli A, Bratt, Debbie L, Bricker, Traci L, Brown, Liliana, Buchser, William J, Carreño, Juan Manuel, Cohen-Lavi, Liel, Darling, Tamarand L, Davis-Gardner, Meredith E, Dearlove, Bethany L, Di, Han, Dittmann, Meike, Doria-Rose, Nicole A, Douek, Daniel C, Drosten, Christian, Edara, Venkata-Viswanadh, Ellebedy, Ali, Fabrizio, Thomas P, Ferrari, Guido, Fischer, Will M, Florence, William C, Fouchier, Ron AM, Franks, John, García-Sastre, Adolfo, Godzik, Adam, Gonzalez-Reiche, Ana Silvia, Gordon, Aubree, Haagmans, Bart L, Halfmann, Peter J, Ho, David D, Holbrook, Michael R, Huang, Yaoxing, James, Sarah L, Jaroszewski, Lukasz, Jeevan, Trushar, Johnson, Robert M, Jones, Terry C, Joshi, Astha, Kawaoka, Yoshihiro, Kercher, Lisa, Koopmans, Marion PG, Korber, Bette, Koren, Eilay, Koup, Richard A, LeGresley, Eric B, Lemieux, Jacob E, Liebeskind, Mariel J, Liu, Zhuoming, Livingston, Brandi, Logue, James P, Luo, Yang, McDermott, Adrian B, McElrath, Margaret J, Meliopoulos, Victoria A, Menachery, Vineet D, Montefiori, David C, Mühlemann, Barbara, Munster, Vincent J, Munt, Jenny E, Nair, Manoj S, Netzl, Antonia, Niewiadomska, Anna M, O’Dell, Sijy, Pekosz, Andrew, Perlman, Stanley, Pontelli, Marjorie C, Rockx, Barry, Rolland, Morgane, Rothlauf, Paul W, Sacharen, Sinai, Scheuermann, Richard H, Schmidt, Stephen D, Schotsaert, Michael, Schultz-Cherry, Stacey, Seder, Robert A, Sedova, Mayya, Sette, Alessandro, Shabman, Reed S, Shen, Xiaoying, Shi, Pei-Yong, Shukla, Maulik, Simon, Viviana, Stumpf, Spencer, Sullivan, Nancy J, Thackray, Larissa B, and Theiler, James

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Biological Sciences, Emerging Infectious Diseases, Pneumonia, Vaccine Related, Pneumonia & Influenza, Infectious Diseases, Biodefense, Immunization, Biotechnology, Prevention, Lung, Prevention of disease and conditions, and promotion of well-being, 2.1 Biological and endogenous factors, 3.4 Vaccines, Aetiology, Infection, Good Health and Well Being, Animals, Biological Evolution, COVID-19, COVID-19 Vaccines, Humans, National Institute of Allergy and Infectious Diseases (U.S.), Pandemics, Pharmacogenomic Variants, SARS-CoV-2, United States, Virulence, General Science & Technology
Abstract

The global emergence of many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants jeopardizes the protective antiviral immunity induced after infection or vaccination. To address the public health threat caused by the increasing SARS-CoV-2 genomic diversity, the National Institute of Allergy and Infectious Diseases within the National Institutes of Health established the SARS-CoV-2 Assessment of Viral Evolution (SAVE) programme. This effort was designed to provide a real-time risk assessment of SARS-CoV-2 variants that could potentially affect the transmission, virulence, and resistance to infection- and vaccine-induced immunity. The SAVE programme is a critical data-generating component of the US Government SARS-CoV-2 Interagency Group to assess implications of SARS-CoV-2 variants on diagnostics, vaccines and therapeutics, and for communicating public health risk. Here we describe the coordinated approach used to identify and curate data about emerging variants, their impact on immunity and effects on vaccine protection using animal models. We report the development of reagents, methodologies, models and notable findings facilitated by this collaborative approach and identify future challenges. This programme is a template for the response to rapidly evolving pathogens with pandemic potential by monitoring viral evolution in the human population to identify variants that could reduce the effectiveness of countermeasures.

Published
2022

5. SARS-CoV-2 requires acidic pH to infect cells

Author

Kreutzberger, Alex J. B., Sanyal, Anwesha, Saminathan, Anand, Bloyet, Louis-Marie, Stumpf, Spencer, Liu, Zhuoming, Ojha, Ravi, Patjas, Markku T., Geneid, Ahmed, Scanavachi, Gustavo, Doyle, Catherine A., Somerville, Elliott, Da Cunha Correia, Ricardo Bango, Di Caprio, Giuseppe, Toppila-Salmi, Sanna, Mäkitie, Antti, Kiessling, Volker, Vapalahti, Olli, Whelan, Sean P. J., Balistreri, Giuseppe, and Kirchhausen, Tom

Published
2022

6. A class II MHC-targeted vaccine elicits immunity against SARS-CoV-2 and its variants

Author

Pishesha, Novalia, Harmand, Thibault J., Rothlauf, Paul W., Praest, Patrique, Alexander, Ryan K., van den Doel, Renate, Liebeskind, Mariel J., Vakaki, Maria A., McCaul, Nicholas, Wijne, Charlotte, Verhaar, Elisha, Pinney, William, Heston, Hailey, Bloyet, Louis-Marie, Pontelli, Marjorie Cornejo, Ilagan, Ma. Xenia G., Lebbink, Robert Jan, Buchser, William J., Wiertz, Emmanuel J. H. J., Whelan, Sean P. J., and Ploegh, Hidde L.

Published
2021

9. N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2

Author

McCallum, Matthew, De Marco, Anna, Lempp, Florian A., Tortorici, M. Alejandra, Pinto, Dora, Walls, Alexandra C., Beltramello, Martina, Chen, Alex, Liu, Zhuoming, Zatta, Fabrizia, Zepeda, Samantha, di Iulio, Julia, Bowen, John E., Montiel-Ruiz, Martin, Zhou, Jiayi, Rosen, Laura E., Bianchi, Siro, Guarino, Barbara, Fregni, Chiara Silacci, Abdelnabi, Rana, Foo, Shi-Yan Caroline, Rothlauf, Paul W., Bloyet, Louis-Marie, Benigni, Fabio, Cameroni, Elisabetta, Neyts, Johan, Riva, Agostino, Snell, Gyorgy, Telenti, Amalio, Whelan, Sean P.J., Virgin, Herbert W., Corti, Davide, Pizzuto, Matteo Samuele, and Veesler, David

Published
2021
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11. Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Competent VSV-SARS-CoV-2 and a Clinical Isolate of SARS-CoV-2

Author

Case, James Brett, Rothlauf, Paul W., Chen, Rita E., Liu, Zhuoming, Zhao, Haiyan, Kim, Arthur S., Bloyet, Louis-Marie, Zeng, Qiru, Tahan, Stephen, Droit, Lindsay, Ilagan, Ma. Xenia G., Tartell, Michael A., Amarasinghe, Gaya, Henderson, Jeffrey P., Miersch, Shane, Ustav, Mart, Sidhu, Sachdev, Virgin, Herbert W., Wang, David, Ding, Siyuan, Corti, Davide, Theel, Elitza S., Fremont, Daved H., Diamond, Michael S., and Whelan, Sean P.J.

Published
2020
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12. Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

Author

Zost, Seth J., Gilchuk, Pavlo, Chen, Rita E., Case, James Brett, Reidy, Joseph X., Trivette, Andrew, Nargi, Rachel S., Sutton, Rachel E., Suryadevara, Naveenchandra, Chen, Elaine C., Binshtein, Elad, Shrihari, Swathi, Chu, Helen Y., Ostrowski, Mario, Didier, Jonathan E., MacRenaris, Keith W., Jones, Taylor, Day, Samuel, Myers, Luke, Lee, F. Eun-Hyung, Nguyen, Doan C., Sanz, Ignacio, Martinez, David R., Rothlauf, Paul W., Bloyet, Louis-Marie, Whelan, Sean P.J., Baric, Ralph S., Thackray, Larissa B., Diamond, Michael S., Carnahan, Robert H., and Crowe, James E., Jr.

Subjects
Identification and classification, Care and treatment, Research, Viral proteins -- Research, Monoclonal antibodies -- Identification and classification, Immunologic research, COVID-19 -- Diagnosis -- Research -- Care and treatment, Immunological research
Abstract

Author(s): Seth J. Zost [sup.1] , Pavlo Gilchuk [sup.1] , Rita E. Chen [sup.2] [sup.3] , James Brett Case [sup.3] , Joseph X. Reidy [sup.1] , Andrew Trivette [sup.1] , [...], Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date.sup.1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes on the basis of their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of advanced antibody discovery platforms. A platform for rapid antibody discovery enabled the isolation of hundreds of human monoclonal antibodies against SARS-CoV-2 and the prioritization of potent antibody candidates for clinical trials in patients with COVID-19.

Published
2020
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16. SARS-CoV-2 uses CD4 to infect T helper lymphocytes

Author

Brunetti, Natalia S, primary, Davanzo, Gustavo G, primary, de Moraes, Diogo, primary, Ferrari, Allan JR, primary, Souza, Gabriela F, primary, Muraro, Stéfanie Primon, primary, Knittel, Thiago L, primary, Boldrini, Vinicius O, primary, Monteiro, Lauar B, primary, Virgílio-da-Silva, João Victor, primary, Profeta, Gerson S, primary, Wassano, Natália S, primary, Nunes Santos, Luana, primary, Carregari, Victor C, primary, Dias, Artur HS, primary, Veras, Flavio P, additional, Tavares, Lucas A, additional, Forato, Julia, additional, Castro, Icaro MS, additional, Silva-Costa, Lícia C, additional, Palma, André C, additional, Mansour, Eli, additional, Ulaf, Raisa G, additional, Bernardes, Ana F, additional, Nunes, Thyago A, additional, Ribeiro, Luciana C, additional, Agrela, Marcus V, additional, Moretti, Maria Luiza, additional, Buscaratti, Lucas I, additional, Crunfli, Fernanda, additional, Ludwig, Raissa G, additional, Gerhardt, Jaqueline A, additional, Munhoz-Alves, Natália, additional, Marques, Ana Maria, additional, Sesti-Costa, Renata, additional, Amorim, Mariene R, additional, Toledo-Teixeira, Daniel A, additional, Parise, Pierina Lorencini, additional, Martini, Matheus Cavalheiro, additional, Bispos-dos-Santos, Karina, additional, Simeoni, Camila L, additional, Granja, Fabiana, additional, Silvestrini, Virgínia C, additional, de Oliveira, Eduardo B, additional, Faca, Vitor M, additional, Carvalho, Murilo, additional, Castelucci, Bianca G, additional, Pereira, Alexandre B, additional, Coimbra, Laís D, additional, Dias, Marieli MG, additional, Rodrigues, Patricia B, additional, Gomes, Arilson Bernardo SP, additional, Pereira, Fabricio B, additional, Santos, Leonilda MB, additional, Bloyet, Louis-Marie, additional, Stumpf, Spencer, additional, Pontelli, Marjorie C, additional, Whelan, Sean, additional, Sposito, Andrei C, additional, Carvalho, Robson F, additional, Vieira, André S, additional, Vinolo, Marco AR, additional, Damasio, André, additional, Velloso, Licio, additional, Figueira, Ana Carolina M, additional, da Silva, Luis LP, additional, Cunha, Thiago Mattar, additional, Nakaya, Helder I, additional, Marques-Souza, Henrique, additional, Marques, Rafael E, additional, Martins-de-Souza, Daniel, additional, Skaf, Munir S, additional, Proenca-Modena, Jose Luiz, additional, Moraes-Vieira, Pedro MM, additional, Mori, Marcelo A, additional, and Farias, Alessandro S, additional

Published
2023
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17. Author response: SARS-CoV-2 uses CD4 to infect T helper lymphocytes

Author

Brunetti, Natalia S, primary, Davanzo, Gustavo G, primary, de Moraes, Diogo, primary, Ferrari, Allan JR, primary, Souza, Gabriela F, primary, Muraro, Stéfanie Primon, primary, Knittel, Thiago L, primary, Boldrini, Vinicius O, primary, Monteiro, Lauar B, primary, Virgílio-da-Silva, João Victor, primary, Profeta, Gerson S, primary, Wassano, Natália S, primary, Nunes Santos, Luana, primary, Carregari, Victor C, primary, Dias, Artur HS, primary, Veras, Flavio P, additional, Tavares, Lucas A, additional, Forato, Julia, additional, Castro, Icaro MS, additional, Silva-Costa, Lícia C, additional, Palma, André C, additional, Mansour, Eli, additional, Ulaf, Raisa G, additional, Bernardes, Ana F, additional, Nunes, Thyago A, additional, Ribeiro, Luciana C, additional, Agrela, Marcus V, additional, Moretti, Maria Luiza, additional, Buscaratti, Lucas I, additional, Crunfli, Fernanda, additional, Ludwig, Raissa G, additional, Gerhardt, Jaqueline A, additional, Munhoz-Alves, Natália, additional, Marques, Ana Maria, additional, Sesti-Costa, Renata, additional, Amorim, Mariene R, additional, Toledo-Teixeira, Daniel A, additional, Parise, Pierina Lorencini, additional, Martini, Matheus Cavalheiro, additional, Bispos-dos-Santos, Karina, additional, Simeoni, Camila L, additional, Granja, Fabiana, additional, Silvestrini, Virgínia C, additional, de Oliveira, Eduardo B, additional, Faca, Vitor M, additional, Carvalho, Murilo, additional, Castelucci, Bianca G, additional, Pereira, Alexandre B, additional, Coimbra, Laís D, additional, Dias, Marieli MG, additional, Rodrigues, Patricia B, additional, Gomes, Arilson Bernardo SP, additional, Pereira, Fabricio B, additional, Santos, Leonilda MB, additional, Bloyet, Louis-Marie, additional, Stumpf, Spencer, additional, Pontelli, Marjorie C, additional, Whelan, Sean, additional, Sposito, Andrei C, additional, Carvalho, Robson F, additional, Vieira, André S, additional, Vinolo, Marco AR, additional, Damasio, André, additional, Velloso, Licio, additional, Figueira, Ana Carolina M, additional, da Silva, Luis LP, additional, Cunha, Thiago Mattar, additional, Nakaya, Helder I, additional, Marques-Souza, Henrique, additional, Marques, Rafael E, additional, Martins-de-Souza, Daniel, additional, Skaf, Munir S, additional, Proenca-Modena, Jose Luiz, additional, Moraes-Vieira, Pedro MM, additional, Mori, Marcelo A, additional, and Farias, Alessandro S, additional

Published
2023
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18. SARS-CoV-2 uses CD4 to infect T helper lymphocytes

Author

Brunetti, Natalia S, Davanzo, Gustavo G, de Moraes, Diogo, Ferrari, Allan JR, Souza, Gabriela F, Muraro, Stéfanie Primon, Knittel, Thiago L, Boldrini, Vinicius O, Monteiro, Lauar B, Virgílio-da-Silva, João Victor, Profeta, Gerson S, Wassano, Natália S, Nunes Santos, Luana, Carregari, Victor C, Dias, Artur HS, Veras, Flavio P, Tavares, Lucas A, Forato, Julia, Castro, Icaro MS, Silva-Costa, Lícia C, Palma, André C, Mansour, Eli, Ulaf, Raisa G, Bernardes, Ana F, Nunes, Thyago A, Ribeiro, Luciana C, Agrela, Marcus V, Moretti, Maria Luiza, Buscaratti, Lucas I, Crunfli, Fernanda, Ludwig, Raissa G, Gerhardt, Jaqueline A, Munhoz-Alves, Natália, Marques, Ana Maria, Sesti-Costa, Renata, Amorim, Mariene R, Toledo-Teixeira, Daniel A, Parise, Pierina Lorencini, Martini, Matheus Cavalheiro, Bispos-dos-Santos, Karina, Simeoni, Camila L, Granja, Fabiana, Silvestrini, Virgínia C, de Oliveira, Eduardo B, Faca, Vitor M, Carvalho, Murilo, Castelucci, Bianca G, Pereira, Alexandre B, Coimbra, Laís D, Dias, Marieli MG, Rodrigues, Patricia B, Gomes, Arilson Bernardo SP, Pereira, Fabricio B, Santos, Leonilda MB, Bloyet, Louis-Marie, Stumpf, Spencer, Pontelli, Marjorie C, Whelan, Sean, Sposito, Andrei C, Carvalho, Robson F, Vieira, André S, Vinolo, Marco AR, Damasio, André, Velloso, Licio, Figueira, Ana Carolina M, da Silva, Luis LP, Cunha, Thiago Mattar, Nakaya, Helder I, Marques-Souza, Henrique, Marques, Rafael E, Martins-de-Souza, Daniel, Skaf, Munir S, Proenca-Modena, Jose Luiz, Moraes-Vieira, Pedro MM, Mori, Marcelo A, Farias, Alessandro S, Brunetti, Natalia S, Davanzo, Gustavo G, de Moraes, Diogo, Ferrari, Allan JR, Souza, Gabriela F, Muraro, Stéfanie Primon, Knittel, Thiago L, Boldrini, Vinicius O, Monteiro, Lauar B, Virgílio-da-Silva, João Victor, Profeta, Gerson S, Wassano, Natália S, Nunes Santos, Luana, Carregari, Victor C, Dias, Artur HS, Veras, Flavio P, Tavares, Lucas A, Forato, Julia, Castro, Icaro MS, Silva-Costa, Lícia C, Palma, André C, Mansour, Eli, Ulaf, Raisa G, Bernardes, Ana F, Nunes, Thyago A, Ribeiro, Luciana C, Agrela, Marcus V, Moretti, Maria Luiza, Buscaratti, Lucas I, Crunfli, Fernanda, Ludwig, Raissa G, Gerhardt, Jaqueline A, Munhoz-Alves, Natália, Marques, Ana Maria, Sesti-Costa, Renata, Amorim, Mariene R, Toledo-Teixeira, Daniel A, Parise, Pierina Lorencini, Martini, Matheus Cavalheiro, Bispos-dos-Santos, Karina, Simeoni, Camila L, Granja, Fabiana, Silvestrini, Virgínia C, de Oliveira, Eduardo B, Faca, Vitor M, Carvalho, Murilo, Castelucci, Bianca G, Pereira, Alexandre B, Coimbra, Laís D, Dias, Marieli MG, Rodrigues, Patricia B, Gomes, Arilson Bernardo SP, Pereira, Fabricio B, Santos, Leonilda MB, Bloyet, Louis-Marie, Stumpf, Spencer, Pontelli, Marjorie C, Whelan, Sean, Sposito, Andrei C, Carvalho, Robson F, Vieira, André S, Vinolo, Marco AR, Damasio, André, Velloso, Licio, Figueira, Ana Carolina M, da Silva, Luis LP, Cunha, Thiago Mattar, Nakaya, Helder I, Marques-Souza, Henrique, Marques, Rafael E, Martins-de-Souza, Daniel, Skaf, Munir S, Proenca-Modena, Jose Luiz, Moraes-Vieira, Pedro MM, Mori, Marcelo A, and Farias, Alessandro S

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent of a major global outbreak of respiratory tract disease known as Coronavirus Disease 2019 (COVID-19). SARS-CoV-2 infects mainly lungs and may cause several immune-related complications, such as lymphocytopenia and cytokine storm, which are associated with the severity of the disease and predict mortality. The mechanism by which SARS-CoV-2 infection may result in immune system dysfunction is still not fully understood. Here, we show that SARS-CoV-2 infects human CD4+ T helper cells, but not CD8+ T cells, and is present in blood and bronchoalveolar lavage T helper cells of severe COVID-19 patients. We demonstrated that SARS-CoV-2 spike glycoprotein (S) directly binds to the CD4 molecule, which in turn mediates the entry of SARS-CoV-2 in T helper cells. This leads to impaired CD4 T cell function and may cause cell death. SARS-CoV-2-infected T helper cells express higher levels of IL-10, which is associated with viral persistence and disease severity. Thus, CD4-mediated SARS-CoV-2 infection of T helper cells may contribute to a poor immune response in COVID-19 patients.

Published
2023

20. BSL2-compliant lethal mouse model of SARS-CoV-2 and variants of concern to evaluate therapeutics targeting the Spike protein

Author

Manangeeswaran, Mohanraj, primary, Ireland, Derek D. C., additional, Thacker, Seth G., additional, Lee, Ha-Na, additional, Kelley-Baker, Logan, additional, Lewkowicz, Aaron P., additional, Rothlauf, Paul W., additional, Cornejo Pontelli, Marjorie, additional, Bloyet, Louis-Marie, additional, Eckhaus, Michael A., additional, Mendoza, Mirian I., additional, Whelan, Sean, additional, and Verthelyi, Daniela, additional

Published
2022
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21. SARS-CoV-2 requires acidic pH to infect cells

Author

Kreutzberger, Alex J.B., primary, Sanyal, Anwesha, additional, Saminathan, Anand, additional, Bloyet, Louis-Marie, additional, Stumpf, Spencer, additional, Liu, Zhuoming, additional, Ojha, Ravi, additional, Patjas, Markku T., additional, Geneid, Ahmed, additional, Scanavachi, Gustavo, additional, Doyle, Catherine A., additional, Somerville, Elliott, additional, Bango Da Cunha Correira, Ricardo, additional, Di Caprio, Giuseppe, additional, Toppila-Salmi, Sanna, additional, Mäkitie, Antti, additional, Kiessling, Volker, additional, Vapalahti, Olli, additional, Whelan, Sean P.J., additional, Balistreri, Giuseppe, additional, and Kirchhausen, Tom, additional

Published
2022
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22. Defining the risk of SARS-CoV-2 variants on immune protection

Author

DeGrace, Marciela M., Ghedin, Elodie, Frieman, Matthew B., Krammer, Florian, Grifoni, Alba, Alisoltani, Arghavan, Alter, Galit, Amara, Rama R., Baric, Ralph S., Barouch, Dan H., Bloom, Jesse D., Bloyet, Louis Marie, Bonenfant, Gaston, Boon, Adrianus C.M., Boritz, Eli A., Bratt, Debbie L., Bricker, Traci L., Brown, Liliana, Buchser, William J., Carreño, Juan Manuel, Cohen-Lavi, Liel, Darling, Tamarand L., Davis-Gardner, Meredith E., Dearlove, Bethany L., Di, Han, Dittmann, Meike, Doria-Rose, Nicole A., Douek, Daniel C., Drosten, Christian, Edara, Venkata Viswanadh, Ellebedy, Ali, Fabrizio, Thomas P., Ferrari, Guido, Fischer, Will M., Florence, William C., Fouchier, Ron A.M., Franks, John, García-Sastre, Adolfo, Godzik, Adam, Gonzalez-Reiche, Ana Silvia, Gordon, Aubree, Haagmans, Bart L., Halfmann, Peter J., Ho, David D., Holbrook, Michael R., Huang, Yaoxing, James, Sarah L., Jaroszewski, Lukasz, Jeevan, Trushar, Johnson, Robert M., Jones, Terry C., Joshi, Astha, Kawaoka, Yoshihiro, Kercher, Lisa, Koopmans, Marion P.G., Korber, Bette, Koren, Eilay, Koup, Richard A., LeGresley, Eric B., Lemieux, Jacob E., Liebeskind, Mariel J., Liu, Zhuoming, Livingston, Brandi, Logue, James P., Luo, Yang, McDermott, Adrian B., McElrath, Margaret J., Meliopoulos, Victoria A., Menachery, Vineet D., Montefiori, David C., Mühlemann, Barbara, Munster, Vincent J., Munt, Jenny E., Nair, Manoj S., Netzl, Antonia, Niewiadomska, Anna M., O’Dell, Sijy, Pekosz, Andrew, Perlman, Stanley, Pontelli, Marjorie C., Rockx, Barry, Rolland, Morgane, Rothlauf, Paul W., Sacharen, Sinai, Scheuermann, Richard H., Schmidt, Stephen D., Schotsaert, Michael, Schultz-Cherry, Stacey, Seder, Robert A., Sedova, Mayya, Sette, Alessandro, Shabman, Reed S., Shen, Xiaoying, Shi, Pei Yong, Shukla, Maulik, Simon, Viviana, Stumpf, Spencer, Sullivan, Nancy J., Thackray, Larissa B., Theiler, James, Thomas, Paul G., Trifkovic, Sanja, Türeli, Sina, Turner, Samuel A., Vakaki, Maria A., van Bakel, Harm, VanBlargan, Laura A., Vincent, Leah R., Wallace, Zachary S., Wang, Li, Wang, Maple, Wang, Pengfei, Wang, Wei, Weaver, Scott C., Webby, Richard J., Weiss, Carol D., Wentworth, David E., Weston, Stuart M., Whelan, Sean P.J., Whitener, Bradley M., Wilks, Samuel H., Xie, Xuping, Ying, Baoling, Yoon, Hyejin, Zhou, Bin, Hertz, Tomer, Smith, Derek J., Diamond, Michael S., Post, Diane J., Suthar, Mehul S., DeGrace, Marciela M., Ghedin, Elodie, Frieman, Matthew B., Krammer, Florian, Grifoni, Alba, Alisoltani, Arghavan, Alter, Galit, Amara, Rama R., Baric, Ralph S., Barouch, Dan H., Bloom, Jesse D., Bloyet, Louis Marie, Bonenfant, Gaston, Boon, Adrianus C.M., Boritz, Eli A., Bratt, Debbie L., Bricker, Traci L., Brown, Liliana, Buchser, William J., Carreño, Juan Manuel, Cohen-Lavi, Liel, Darling, Tamarand L., Davis-Gardner, Meredith E., Dearlove, Bethany L., Di, Han, Dittmann, Meike, Doria-Rose, Nicole A., Douek, Daniel C., Drosten, Christian, Edara, Venkata Viswanadh, Ellebedy, Ali, Fabrizio, Thomas P., Ferrari, Guido, Fischer, Will M., Florence, William C., Fouchier, Ron A.M., Franks, John, García-Sastre, Adolfo, Godzik, Adam, Gonzalez-Reiche, Ana Silvia, Gordon, Aubree, Haagmans, Bart L., Halfmann, Peter J., Ho, David D., Holbrook, Michael R., Huang, Yaoxing, James, Sarah L., Jaroszewski, Lukasz, Jeevan, Trushar, Johnson, Robert M., Jones, Terry C., Joshi, Astha, Kawaoka, Yoshihiro, Kercher, Lisa, Koopmans, Marion P.G., Korber, Bette, Koren, Eilay, Koup, Richard A., LeGresley, Eric B., Lemieux, Jacob E., Liebeskind, Mariel J., Liu, Zhuoming, Livingston, Brandi, Logue, James P., Luo, Yang, McDermott, Adrian B., McElrath, Margaret J., Meliopoulos, Victoria A., Menachery, Vineet D., Montefiori, David C., Mühlemann, Barbara, Munster, Vincent J., Munt, Jenny E., Nair, Manoj S., Netzl, Antonia, Niewiadomska, Anna M., O’Dell, Sijy, Pekosz, Andrew, Perlman, Stanley, Pontelli, Marjorie C., Rockx, Barry, Rolland, Morgane, Rothlauf, Paul W., Sacharen, Sinai, Scheuermann, Richard H., Schmidt, Stephen D., Schotsaert, Michael, Schultz-Cherry, Stacey, Seder, Robert A., Sedova, Mayya, Sette, Alessandro, Shabman, Reed S., Shen, Xiaoying, Shi, Pei Yong, Shukla, Maulik, Simon, Viviana, Stumpf, Spencer, Sullivan, Nancy J., Thackray, Larissa B., Theiler, James, Thomas, Paul G., Trifkovic, Sanja, Türeli, Sina, Turner, Samuel A., Vakaki, Maria A., van Bakel, Harm, VanBlargan, Laura A., Vincent, Leah R., Wallace, Zachary S., Wang, Li, Wang, Maple, Wang, Pengfei, Wang, Wei, Weaver, Scott C., Webby, Richard J., Weiss, Carol D., Wentworth, David E., Weston, Stuart M., Whelan, Sean P.J., Whitener, Bradley M., Wilks, Samuel H., Xie, Xuping, Ying, Baoling, Yoon, Hyejin, Zhou, Bin, Hertz, Tomer, Smith, Derek J., Diamond, Michael S., Post, Diane J., and Suthar, Mehul S.

Abstract

The global emergence of many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants jeopardizes the protective antiviral immunity induced after infection or vaccination. To address the public health threat caused by the increasing SARS-CoV-2 genomic diversity, the National Institute of Allergy and Infectious Diseases within the National Institutes of Health established the SARS-CoV-2 Assessment of Viral Evolution (SAVE) programme. This effort was designed to provide a real-time risk assessment of SARS-CoV-2 variants that could potentially affect the transmission, virulence, and resistance to infection- and vaccine-induced immunity. The SAVE programme is a critical data-generating component of the US Government SARS-CoV-2 Interagency Group to assess implications of SARS-CoV-2 variants on diagnostics, vaccines and therapeutics, and for communicating public health risk. Here we describe the coordinated approach used to identify and curate data about emerging variants, their impact on immunity and effects on vaccine protection using animal models. We report the development of reagents, methodologies, models and notable findings facilitated by this collaborative approach and identify future challenges. This programme is a template for the response to rapidly evolving pathogens with pandemic potential by monitoring viral evolution in the human population to identify variants that could reduce the effectiveness of countermeasures.

Published
2022

24. Longitudinal Study after Sputnik V Vaccination Shows Durable SARS-CoV-2 Neutralizing Antibodies and Reduced Viral Variant Escape to Neutralization over Time

Author

Gonzalez Lopez Ledesma, María M., primary, Sanchez, Lautaro, additional, Ojeda, Diego S., additional, Oviedo Rouco, Santiago, additional, Rossi, Andrés H., additional, Varese, Augusto, additional, Mazzitelli, Ignacio, additional, Pascuale, Carla A., additional, Miglietta, Esteban A., additional, Rodríguez, Pamela E., additional, Pallarés, Horacio M., additional, Costa Navarro, Guadalupe S., additional, Caramelo, Julio J., additional, Rothlauf, Paul W., additional, Liu, Zhuoming, additional, Bloyet, Louis-Marie, additional, Cornejo Pontelli, Marjorie, additional, Rasetto, Natali B., additional, Wenker, Shirley D., additional, Ramis, Lila Y., additional, Bialer, Magalí G., additional, de Leone, María Jose, additional, Hernando, C. Esteban, additional, Bianchimano, Luciana, additional, Ríos, Antonella S., additional, Treffinger Cienfuegos, María Soledad, additional, Rodriguez García, Diana R., additional, Longueira, Yesica, additional, Laufer, Natalia, additional, Alvarez, Diego, additional, Ceballos, Ana, additional, Ochoa, Valeria, additional, Monzani, Cecilia, additional, Turk, Gariela, additional, Salvatori, Melina, additional, Carradori, Jorge, additional, Prost, Katherine, additional, Rima, Alejandra, additional, Varela, Claudia, additional, Ercole, Regina, additional, Toro, Rosana I., additional, Gutierrez, Sebastian, additional, Zubieta, Martín, additional, Acuña, Dolores, additional, Nabaes Jodar, Mercedes S., additional, Torres, Carolina, additional, Mojsiejczuk, Laura, additional, Viegas, Mariana, additional, Velazquez, Pilar, additional, Testa, Clarisa, additional, Kreplak, Nicolas, additional, Yanovsky, Marcelo, additional, Whelan, Sean, additional, Geffner, Jorge, additional, Pifano, Marina, additional, and Gamarnik, Andrea V., additional

Published
2022
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27. Replication-Competent Vesicular Stomatitis Virus Vaccine Vector Protects against SARS-CoV-2-Mediated Pathogenesis in Mice

Author

Case, James Brett, Rothlauf, Paul W., Chen, Rita E., Kafai, Natasha M., Fox, Julie M., Smith, Brittany K., Shrihari, Swathi, McCune, Broc T., Harvey, Ian B., Keeler, Shamus P., Bloyet, Louis-Marie, Zhao, Haiyan, Ma, Meisheng, Adams, Lucas J., Winkler, Emma S., Holtzman, Michael J., Fremont, Daved H., Whelan, Sean P.J., and Diamond, Michael S.

Published
2020
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28. Longitudinal Study after Sputnik V Vaccination Shows Durable SARS-CoV-2 Neutralizing Antibodies and Reduced Viral Variant Escape over Time

Author

Gonzalez Lopez Ledesma, María M., primary, Sanchez, Lautaro, additional, Ojeda, Diego S., additional, Rouco, Santiago Oviedo, additional, Rossi, Andres H., additional, Varese, Augusto, additional, Mazzitelli, Ignacio, additional, Pascuale, Carla A., additional, Miglietta, Esteban A., additional, Rodríguez, Pamela E., additional, Pallarés, Horacio M., additional, Costa Navarro, Guadalupe S., additional, Caramelo, Julio J., additional, Rothlauf, Paul W, additional, Liu, Zhuoming, additional, Bloyet, Louis-Marie, additional, Pontelli, Marjorie Cornejo, additional, Rasetto, Natali B., additional, Wenker, Shirley D., additional, Ramis, Lila Y., additional, Bialer, Magalí G., additional, Jose de Leone, María, additional, Hernando, C. Esteban, additional, Bianchimano, Luciana, additional, Rios, Antonella, additional, Treffinger Cienfuegos, María Soledad, additional, Rodriguez García, Diana R., additional, Longueira, Yesica, additional, Laufer, Natalia, additional, Alvarez, Diego, additional, Ceballos, Ana, additional, Ochoa, Valeria, additional, Monzani, Cecilia, additional, Turk, Gariela, additional, Salvatori, Melina, additional, Carradori, Jorge, additional, Prost, Katherine, additional, Rima, Alejandra, additional, Varela, Claudia, additional, Ercole, Regina, additional, Toro, Rosana I., additional, Gutierrez, Sebastian, additional, Zubieta, Martín, additional, Acuña, Dolores, additional, Nabaes Jodar, Mercedes S., additional, Torres, Carolina, additional, Mojsiejczuk, Laura, additional, Viegas, Mariana, additional, Velazquez, Pilar, additional, Testa, Clarisa, additional, Kreplak, Nicolas, additional, Yanovsky, Marcelo, additional, Whelan, Sean, additional, Geffner, Jorge, additional, Pifano, Marina, additional, and Gamarnik, Andrea V., additional

Published
2021
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29. A class II MHC-targeted vaccine elicits immunity against SARS-CoV-2 and its variants

Author

MMB Research line 3a, Infection & Immunity, Pishesha, Novalia, Harmand, Thibault J, Rothlauf, Paul W, Praest, Patrique, Alexander, Ryan K, van den Doel, Renate, Liebeskind, Mariel J, Vakaki, Maria A, McCaul, Nicholas, Wijne, Charlotte, Verhaar, Elisha, Pinney, William, Heston, Hailey, Bloyet, Louis-Marie, Pontelli, Marjorie Cornejo, Ilagan, Ma Xenia G, Jan Lebbink, Robert, Buchser, William J, Wiertz, Emmanuel J H J, Whelan, Sean P J, Ploegh, Hidde L, MMB Research line 3a, Infection & Immunity, Pishesha, Novalia, Harmand, Thibault J, Rothlauf, Paul W, Praest, Patrique, Alexander, Ryan K, van den Doel, Renate, Liebeskind, Mariel J, Vakaki, Maria A, McCaul, Nicholas, Wijne, Charlotte, Verhaar, Elisha, Pinney, William, Heston, Hailey, Bloyet, Louis-Marie, Pontelli, Marjorie Cornejo, Ilagan, Ma Xenia G, Jan Lebbink, Robert, Buchser, William J, Wiertz, Emmanuel J H J, Whelan, Sean P J, and Ploegh, Hidde L

Published
2021

31. N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2

Author

McCallum, Matthew, primary, Marco, Anna De, additional, Lempp, Florian, additional, Tortorici, M. Alejandra, additional, Pinto, Dora, additional, Walls, Alexandra C., additional, Beltramello, Martina, additional, Chen, Alex, additional, Liu, Zhuoming, additional, Zatta, Fabrizia, additional, Zepeda, Samantha, additional, di Iulio, Julia, additional, Bowen, John E., additional, Montiel-Ruiz, Martin, additional, Zhou, Jiayi, additional, Rosen, Laura E., additional, Bianchi, Siro, additional, Guarino, Barbara, additional, Fregni, Chiara Silacci, additional, Abdelnabi, Rana, additional, Caroline Foo, Shi-Yan, additional, Rothlauf, Paul W., additional, Bloyet, Louis-Marie, additional, Benigni, Fabio, additional, Cameroni, Elisabetta, additional, Neyts, Johan, additional, Riva, Agostino, additional, Snell, Gyorgy, additional, Telenti, Amalio, additional, Whelan, Sean P.J., additional, Virgin, Herbert W., additional, Corti, Davide, additional, Pizzuto, Matteo Samuele, additional, and Veesler, David, additional

Published
2021
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33. Landscape analysis of escape variants identifies SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization

Author

Liu, Zhuoming, primary, VanBlargan, Laura A., additional, Bloyet, Louis-Marie, additional, Rothlauf, Paul W., additional, Chen, Rita E., additional, Stumpf, Spencer, additional, Zhao, Haiyan, additional, Errico, John M., additional, Theel, Elitza S., additional, Liebeskind, Mariel J., additional, Alford, Brynn, additional, Buchser, William J., additional, Ellebedy, Ali H., additional, Fremont, Daved H., additional, Diamond, Michael S., additional, and Whelan, Sean P. J., additional

Published
2020
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34. SARS-CoV-2 Uses CD4 to Infect T Helper Lymphocytes

Author

Brunetti, Natália S., primary, Davanzo, Gustavo G., additional, de Moraes, Diogo, additional, Ferrari, Allan J. R., additional, de Souza, Gabriela F., additional, Muraro, Stefanie P., additional, Knittel, Thiago L., additional, Boldrini, Vinícius O., additional, Monteiro, Lauar B., additional, Virgilio-da-Silva, João Victor, additional, Profeta, Gerson S., additional, Wassano, Natália S., additional, Santos, Luana N., additional, Carregari, Victor C., additional, Dias, Artur H. S., additional, Veras, Flavio P., additional, Tavares, Lucas A., additional, Forato, Julia, additional, Castro, Ícaro, additional, Silva-Costa, Lícia C., additional, Palma, Andre, additional, Mansour, Eli, additional, Ulaf, Raisa G., additional, Bernardes, Ana F., additional, Nunes, Thyago A., additional, Ribeiro, Luciana C., additional, Agrela, Marcus V., additional, Moretti, Maria Luiza, additional, Buscaratti, Lucas I., additional, Crunfli, Fernanda, additional, Ludwig, Raissa G., additional, Gerhardt, Jaqueline A., additional, Munhoz-Alves, Natália, additional, Marques, Ana M., additional, Sesti-Costa, Renata, additional, Amorim, Mariene R., additional, Texeira, Daniel A. T., additional, Parise, Pierina L., additional, Martini, Matheus C., additional, Bispo-dos-Santos, Karina, additional, Simeoni, Camila L., additional, Granja, Fabiana, additional, Silvestrini, Virginia C., additional, de Oliveira, Eduardo B., additional, Faça, Vitor M., additional, Carvalho, Murilo, additional, Castelucci, Bianca G., additional, Pereira, Alexandre B., additional, Coimbra, Laís D., additional, Dias, Marieli M. G., additional, Rodrigues, Patricia B., additional, S. P. Gomes, Arilson Bernardo, additional, Pereira, Fabricio B., additional, Santos, Leonilda M. B., additional, Bloyet, Louis-Marie, additional, Stumpf, Spencer, additional, Pontelli, Marjorie C., additional, Whelan, Sean P. J., additional, Sposito, Andrei C., additional, Carvalho, Robson F., additional, Vieira, Andre S., additional, Vinolo, Marco A. R., additional, Damasio, André, additional, Velloso, Licio A., additional, M. Figueira, Ana Carolina, additional, da Silva, Luis L. P., additional, Cunha, Thiago M., additional, Nakaya, Helder I., additional, Marques-Souza, Henrique, additional, Marques, Rafael E., additional, Martins-de-Souza, Daniel, additional, Skaf, Munir S., additional, Proença-Modena, José Luiz, additional, Moraes-Vieira, Pedro M., additional, Mori, Marcelo A., additional, and Farias, Alessandro S., additional

Published
2020
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35. Replication-competent vesicular stomatitis virus vaccine vector protects against SARS-CoV-2-mediated pathogenesis

Author

Case, James Brett, primary, Rothlauf, Paul W., additional, Chen, Rita E., additional, Kafai, Natasha M., additional, Fox, Julie M., additional, Shrihari, Swathi, additional, McCune, Broc T., additional, Harvey, Ian B., additional, Smith, Brittany, additional, Keeler, Shamus P., additional, Bloyet, Louis-Marie, additional, Winkler, Emma S., additional, Holtzman, Michael J., additional, Fremont, Daved H., additional, Whelan, Sean P.J., additional, and Diamond, Michael S., additional

Published
2020
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37. Neutralizing antibody and soluble ACE2 inhibition of a replication-competent VSV-SARS-CoV-2 and a clinical isolate of SARS-CoV-2

Author

Case, James Brett, primary, Rothlauf, Paul W., additional, Chen, Rita E., additional, Liu, Zhuoming, additional, Zhao, Haiyan, additional, Kim, Arthur S., additional, Bloyet, Louis-Marie, additional, Zeng, Qiru, additional, Tahan, Stephen, additional, Droit, Lindsay, additional, Ilagan, Ma. Xenia G., additional, Tartell, Michael A., additional, Amarasinghe, Gaya, additional, Henderson, Jeffrey P., additional, Miersch, Shane, additional, Ustav, Mart, additional, Sidhu, Sachdev, additional, Virgin, Herbert W., additional, Wang, David, additional, Ding, Siyuan, additional, Corti, Davide, additional, Theel, Elitza S., additional, Fremont, Daved H., additional, Diamond, Michael S., additional, and Whelan, Sean P.J., additional

Published
2020
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38. The C Protein Is Recruited to Measles Virus Ribonucleocapsids by the Phosphoprotein

Author

Pfaller, Christian K., primary, Bloyet, Louis-Marie, additional, Donohue, Ryan C., additional, Huff, Amanda L., additional, Bartemes, William P., additional, Yousaf, Iris, additional, Urzua, Erica, additional, Clavière, Mathieu, additional, Zachary, Marie, additional, de Masson d’Autume, Valentin, additional, Carson, Sandra, additional, Schieferecke, Adam J., additional, Meyer, Alyssa J., additional, Gerlier, Denis, additional, and Cattaneo, Roberto, additional

Published
2020
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43. Interference with the production of infectious viral particles and bimodal inhibition of replication are broadly conserved antiviral properties of IFITMs

Author

Tartour, Kevin, primary, Nguyen, Xuan-Nhi, additional, Appourchaux, Romain, additional, Assil, Sonia, additional, Barateau, Véronique, additional, Bloyet, Louis-Marie, additional, Burlaud Gaillard, Julien, additional, Confort, Marie-Pierre, additional, Escudero-Perez, Beatriz, additional, Gruffat, Henri, additional, Hong, Saw See, additional, Moroso, Marie, additional, Reynard, Olivier, additional, Reynard, Stéphanie, additional, Decembre, Elodie, additional, Ftaich, Najate, additional, Rossi, Axel, additional, Wu, Nannan, additional, Arnaud, Frédérick, additional, Baize, Sylvain, additional, Dreux, Marlène, additional, Gerlier, Denis, additional, Paranhos-Baccala, Glaucia, additional, Volchkov, Viktor, additional, Roingeard, Philippe, additional, and Cimarelli, Andrea, additional

Published
2017
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44. Formation et régulation du complexe polymérase du virus de la rougeole

Author

Bloyet, Louis-Marie, Immunobiologie des infections virales – Immunobiology of Viral Infections (IbIV), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ecole normale supérieure de lyon - ENS LYON, Denis Gerlier, Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
viruses, Phosphoprotein, Polymérase, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, HSP90, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Phosphoprotéine, C protein, Protéine C, Rougeole, Polymerase, Virus, Measles
Abstract

The Mononegavirales order contains several pathogens like measles, rabies, mumps and Ebola viruses. These viruses share numerous homologous molecular mechanisms and in particular they have a highly conserved RNA synthesis machinery that is unique in the living world. Indeed, the polymerase complex, composed of the polymerase (L) and the phosphoprotein (P), uses a template of RNA recovered by a sheath made of nucleoproteins. The formation of the complex was investigated and the chaperone protein HSP90 (Heat Shock Protein of 90 kD) was shown to be required for the formation of the complex. The inhibition of HSP90 activity induces the ubiquitinylation and the degradation of the L protein by the proteasome. Both P and HSP90 are required to form stable, soluble and functional polymerase complexes. The domains of P involved in the formation of the complex have been mapped and they show that the interplay between P and L is complex with at least three identified functions: binding, folding and function of the polymerase complex. Finally, an interaction between P and the viral C protein, known to inhibit the viral RNA synthesis, have been identified, mapped and allows new perspectives concerning the molecular mechanism underlying its inhibitory effect.; L’ordre viral des Mononegavirales contient de nombreux virus pathogènes tels que le virus de la rougeole, le virus de la rage, le virus des oreillons ou encore le virus Ebola. Ces virus mettent tous en place des mécanismes moléculaires similaires, notamment concernant la synthèse des ARN viraux. Le complexe polymérase, composé de la polymérase virale (L) et de la phosphoprotéine (P), possède un fonctionnement encore obscur et unique dans le monde du vivant, notamment car il utilise une matrice enchâssée dans une gaine protéique. La formation de ce complexe a été étudiée et la protéine chaperon HSP90 (« Heat Shock Protein of 90 kD ») s’est révélée nécessaire à la formation du complexe. L’inhibition de l’activité d’HSP90 entraine l’ubiquitination et la dégradation de la protéine L par le protéasome. Les protéines P et HSP90 sont toutes les deux nécessaires au repliement de la protéine L et à la formation d’un complexe P-L stable, soluble et fonctionnel. Les domaines de P impliqués dans la formation du complexe, ont également été cartographiés et révèlent des interactions complexes entre P et L, mêlant liaison, stabilisation, repliement et fonction. Enfin, une interaction entre P et la protéine virale C, connue pour inhiber la synthèse des ARN viraux, a été identifiée, cartographiée et ouvre des perspectives quant aux mécanismes moléculaires sous-jacents à son effet inhibiteur.

Published
2015

47. Dissecting virus entry: replication-independent analysis of virus binding, internalization, and penetration using minimal complementation of β-galactosidase

Author

Burkard, Christine, Bloyet, Louis Marie, Wicht, Oliver, Van Kuppeveld, Frank J., Rottier, Peter J M, De Haan, Cornelis A M, Bosch, Berend Jan, LS Virologie, I&I SIB1, Strategic Infection Biology, LS Virologie, I&I SIB1, and Strategic Infection Biology

Subjects
RNA viruses, Coronaviruses, viruses, medicine.disease_cause, Virus Replication, Giant Cells, Mice, Viral Envelope Proteins, Genes, Reporter, Internalization, media_common, Coronavirus, 0303 health sciences, Multidisciplinary, Cell fusion, 030302 biochemistry & molecular biology, General Medicine, 3. Good health, Vesicular stomatitis virus, Medical Microbiology, Viral Pathogens, Host-Pathogen Interactions, Viruses, Medicine, General Agricultural and Biological Sciences, Research Article, Viral Entry, media_common.quotation_subject, Science, Virus Attachment, Biology, Microbiology, Viral Attachment, General Biochemistry, Genetics and Molecular Biology, Virus, Rhabdoviruses, Cell Line, 03 medical and health sciences, Viral envelope, Viral entry, Virology, medicine, Animals, Humans, Microbial Pathogens, 030304 developmental biology, Murine hepatitis virus, Biology and life sciences, Host Cells, Organisms, Vesiculovirus, Virus Internalization, biology.organism_classification, beta-Galactosidase, Viral replication, Cats, Viral Transmission and Infection
Abstract

Studies of viral entry into host cells often rely on the detection of post-entry parameters, such as viral replication or the expression of a reporter gene, rather than on measuring entry per se. The lack of assays to easily detect the different steps of entry severely hampers the analysis of this key process in virus infection. Here we describe novel, highly adaptable viral entry assays making use of minimal complementation of the E. coli β-galactosidase in mammalian cells. Enzyme activity is reconstituted when a small intravirion peptide (α-peptide) is complementing the inactive mutant form ΔM15 of β-galactosidase. The method allows to dissect and to independently detect binding, internalization, and fusion of viruses during host cell entry. Here we use it to confirm and extend current knowledge on the entry process of two enveloped viruses: vesicular stomatitis virus (VSV) and murine hepatitis coronavirus (MHV).

Published
2014

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Author

Burkard, Christine, Bloyet, Louis Marie, Wicht, Oliver, Van Kuppeveld, Frank J., Rottier, Peter J M, De Haan, Cornelis A M, Bosch, Berend Jan, Burkard, Christine, Bloyet, Louis Marie, Wicht, Oliver, Van Kuppeveld, Frank J., Rottier, Peter J M, De Haan, Cornelis A M, and Bosch, Berend Jan

Published
2014

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Author

LS Virologie, I&I SIB1, Strategic Infection Biology, Burkard, Christine, Bloyet, Louis Marie, Wicht, Oliver, Van Kuppeveld, Frank J., Rottier, Peter J M, De Haan, Cornelis A M, Bosch, Berend Jan, LS Virologie, I&I SIB1, Strategic Infection Biology, Burkard, Christine, Bloyet, Louis Marie, Wicht, Oliver, Van Kuppeveld, Frank J., Rottier, Peter J M, De Haan, Cornelis A M, and Bosch, Berend Jan

Published
2014

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