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4. Isolation of Human Small Extracellular Vesicles and Tracking of their Uptake by Retinal Pigment Epithelial Cells In Vitro

Author

Marcu, Irene C., Eberhard, Naja, Yerly, Anaïs, Balmer, Verena, Hemphill, Andrew, Mogel, Helga, Gaschen, Véronique, Stoffel, Michael H., and Bluteau, Jasmin

Subjects
PKH, T. gondii, 630 Agriculture, retinal pigment epithelium, Biological Transport, 610 Medicine & health, small extracellular vesicles, Article, lcsh:Chemistry, Extracellular Vesicles, HEK293 Cells, lcsh:Biology (General), lcsh:QD1-999, BODIPY, Humans, 570 Life sciences, biology, sense organs, lcsh:QH301-705.5, Cells, Cultured, EV
Abstract

Small extracellular vesicles (EVs) are among the most frequently investigated EVs and play major roles in intercellular communication by delivering various cargo molecules to target cells. They could potentially represent an alternative delivery strategy to treat ocular toxoplasmosis, a parasitosis affecting the retinal pigment epithelium (RPE). To date, the uptake of human small EVs by RPE cells has never been reported. In this study, we report on the intracellular uptake of fluorescently labelled human urine and fibroblast-derived small EVs by human RPE cells. In summary, both dye-labelled urinary small EVs and small EVs obtained from fibroblasts stably expressing membrane-bound green fluorescent protein were successfully internalized by RPE cells as revealed by immunohistochemistry. In recipient ARPE19 cells, BODIPY-labelled small EVs were found in close vicinity to the parasite Toxoplasma gondii. Additionally, an ultrastructural method was enabled to distinguish between labelled exogenous and endogenous small EVs within target cells.

Published
2020

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