43 results on '"Bohnen, J M"'
Search Results
2. Optimising decision making on illness absenteeism due to fever and common infections within childcare centres: development of a multicomponent intervention and study protocol of a cluster randomised controlled trial
- Author
-
Peetoom, K. K. B., primary, Crutzen, R., additional, Bohnen, J. M. H. A., additional, Verhoeven, R., additional, Nelissen-Vrancken, H. J. M. G., additional, Winkens, B., additional, Dinant, G. J., additional, and Cals, J. W. L., additional
- Published
- 2017
- Full Text
- View/download PDF
3. Optimizing decision-making among childcare staff on fever and common infections: cluster randomized controlled trial.
- Author
-
Peetoom, K K B, Crutzen, R, Verhoeven, R, Bohnen, J M H A, Winkens, B, Dinant, G J, and Cals, J W L
- Subjects
EDUCATION of physicians ,ATTITUDE (Psychology) ,CHILD care workers ,CLUSTER analysis (Statistics) ,FEVER ,HEALTH ,INFECTION ,INTERNET ,MEDICAL personnel ,PROFESSIONS ,RISK perception ,SELF-efficacy ,INFORMATION resources ,DECISION making in clinical medicine ,RANDOMIZED controlled trials ,PSYCHOLOGY - Abstract
Background Children 0–4 years attending childcare are more prone to acquire infections than home-cared children. Childcare illness absenteeism due to fever is mostly driven by fear towards fever in childcare staff and parents. This may cause high childcare absenteeism, healthcare service use, and work absenteeism in parents. This study evaluates a multicomponent intervention targeting determinants of decision-making among childcare staff on illness absenteeism due to fever and common infections. Methods The multicomponent intervention was developed based on the Intervention Mapping approach and consisted of (i) an educational session, (ii) a decision tool, (iii) an information booklet and (iv) an online video. The intervention was evaluated in a cluster randomized controlled trial in Southern Netherlands. Nine centres received the intervention and nine provided childcare-as-usual. Primary outcome measure was the percentage of illness absenteeism on cluster level, defined as number of childcare days absent due to illness on total of registered childcare contract days in a 12-week period. Secondary outcome measures included intended behaviour, attitude, risk perception, knowledge and self-efficacy of childcare staff. Outcomes were analyzed using linear mixed models accounting for clustering. Knowledge was descriptively analysed. Results Overall illness absenteeism was comparable in intervention (2.95%) and control group (2.52%). Secondary outcomes showed significant improvements in intervention group regarding intended behaviour, two of three attitude dimensions. Knowledge increased compared with control but no differences regarding self-efficacy. Conclusion The intervention was not effective in reducing illness absenteeism. However, the intervention improved determinants of decision-making such as intended behaviour, attitude, and knowledge on fever. Trial registration NTR6402 (registered on 21 April 2017). [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
4. Unilamellar liposomes modulate secretion of tumor necrosis factor by lipopolysaccharide-stimulated macrophages
- Author
-
Brisseau, G F, primary, Kresta, A, additional, Schouten, D, additional, Bohnen, J M, additional, Shek, P N, additional, Fok, E, additional, and Rotstein, O D, additional
- Published
- 1994
- Full Text
- View/download PDF
5. Distribution of Free and Liposome-encapsulated Cefoxitin in Experimental Intra-abdominal Sepsis in Rats
- Author
-
Kresta, A, primary, Shek, P N, additional, Odumeru, J, additional, and Bohnen, J M A, additional
- Published
- 1993
- Full Text
- View/download PDF
6. IMPROVED OUTCOME PREDICTION MODEL WITH APACHE-II SCORE DERIVED FROM 777 INTRA-ABDOMINAL INFECTIONS (IAI)
- Author
-
Wittmann, D H, primary, Aprahamian, C, additional, Quebbeman, E J, additional, Bansal, N, additional, Bohnen, J M, additional, Mustard, R A, additional, Shouten, B O, additional, and Nystroem, P-O, additional
- Published
- 1993
- Full Text
- View/download PDF
7. Clinical and scientific importance of source control in abdominal infections: summary of a symposium.
- Author
-
Bohnen, John M.A., Marshall, John C., Fry, Donald E., Johnson, Steven B., Solomkin, Joseph S., Bohnen, J M, Marshall, J C, Fry, D E, Johnson, S B, and Solomkin, J S
- Subjects
MEDICAL societies ,MICROBIAL contamination ,ABDOMEN ,INFECTION ,PREVENTION - Abstract
In May 1997, a panel of surgeon-investigators met to discuss the clinical importance and research implications of controlling the source of abdominal infections. It was concluded that source control is critical to therapeutic success and that antimicrobial therapy and other adjunctive interventions will fail if the source of infection is not controlled by resection, exteriorization or other means. The panelists presented different definitions of source control, depending on the scientific purpose of the definition. All participants agreed that failure to consider the adequacy of source control of infection has limited the value of most clinical trials of therapeutic anti-infective agents. Besides recognizing source control as an essential goal of patient care, the panelists emphasized the need for further investigative work to define, record and stratify the adequacy of source control in clinical trials of therapeutic agents for abdominal infections. [ABSTRACT FROM AUTHOR]
- Published
- 1999
8. Correspondence.
- Author
-
Bohnen, J. M., Christou, N. V., Maclean, L. D., Meakins, J. L., Pollock, A. V., Almgren, B., Watkins, R. M., Watkin, E. M., Mansfield, Averil O., Bradley, J. W. P., Cooperberg, P., Stoller, J. L., McKay, A. J., Macfarlane, I. A., Howat, J. M. T., Hodgson, W. J. B., Nicholls, R. J., Poston, G. J., Pickering, B. N., and Rahamim, J.
- Published
- 1983
- Full Text
- View/download PDF
9. Pharmacodynamics of Antimicrobial Therapy in Surgery
- Author
-
DiPiro, J. T., Edminston, C. E., and Bohnen, J. M. A.
- Published
- 1996
- Full Text
- View/download PDF
10. Pathogenicity of Enterococci in a Rat Model of Fecal Peritonitis
- Author
-
Matlow, A. G., primary, Bohnen, J. M. A., additional, Nohr, C., additional, Christou, N., additional, and Meakins, J., additional
- Published
- 1989
- Full Text
- View/download PDF
11. Abstracts of presentations to the Annual Meetings of the Canadian Society of Colon and Rectal Surgeons Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons: Canadian Surgery Forum, London, Ont., Sept. 19 to 22, 2002.
- Author
-
Asano TK, McLeod RS, Blitz M, Butts C, Kneteman N, Bigam D, Oosthuizen JFM, Phang PT, Gouthro D, Ravid A, Liu M, O'Connor BI, MacRae HM, Cohen Z, McLeod RS, Al-Obeed O, Penning J, Stern HS, Colquhoun P, Nogueras J, Dipasquale B, Petras J, Wexner S, Woodhouse S, Raval MJ, Heine JA, May GR, Bass S, Brown CJ, MacLean AR, Asano T, Cohen Z, MacRae HM, O'Connor BI, McLeod RS, Asano TK, Toma D, Stern HS, McLeod RS, Irshad K, Ghitulescu GA, Gordon PH, MacLean AR, Lilly L, Cohen Z, O'Connor B, McLeod RS, Ravid A, O'Connor BI, Liu M, MacRae HM, Cohen Z, McLeod RS, St Germaine RL, de Gara CJ, Fox R, Kenwell Z, Blitz S, Wong JT, Mc-Mulkin HM, Porter GA, Jayaraman S, Gray D, Burpee SE, Schlachta CM, Mamazza J, Pace K, Poulin EC, Freeman J, Tranqui P, Trottier D, Bodurtha A, Sarma A, Bheerappa N, Sastry RA, de Gara CJ, Hanson J, Hamilton S, Taylor MC, Haase E, Stevens J, Rigo V, Richards J, Bigam DL, Cheung PY, Burpee SE, Schlachta CM, Mamazza J, Pace K, Poulin EC, Grace DM, Gupta S, Sarma A, Bheerappa N, Radhakrishna P, Sastry RA, Malik S, Duffy P, Schulte P, Cameron R, Pace KT, Dyer S, Phan V, Poulin E, Schlachta C, Mamazza J, Stewart R, Honey RJ, Kanthan R, Kanthan SC, Jayaraman S, Aarts MA, Solomon MJ, McLeod RS, Ong S, Pitt D, Stephen W, Latulippe J, Girotti M, Bloom S, Pace K, Dyer S, Stewart R, Honey RJ, Poulin E, Schlachta C, Mamazza J, Furlan JC, Rosen IB, Asano TK, Haigh PI, McLeod RS, Al Saleh N, Taylor B, Karimuddin AA, Marschall J, McFadden A, Pollett WG, Dicks E, Tranqui P, Trottier D, Freeman J, Bodurtha A, Urbach DR, Bell CM, Austin PC, Cleary SP, Gyfe R, Greig P, Smith L, Mackenzie R, Strasberg S, Hanna S, Taylor B, Langer B, Gallinger S, Marschall J, Nechala P, Chibbar R, Colquhoun P, Zhou J, Lee TDG, Meneghetti AT, McKenna GJ, Owen D, Scudamore CH, McMaster RM, Chung SW, Aarts MA, Granton J, Cook DJ, Bohnen JMA, Marshall JC, Colquhoun P, Weiss E, Efron J, Nogueras J, Vernava A, Wexner S, Poulin EC, Schlachta CM, Burpee SE, Pace KT, Mamazza J, Rosen IB, Furlan JC, Charghi R, Schricker T, Backman S, Rouah F, Christou NV, Obayan A, Keith R, Juurlink BHJ, Skaro AI, Liwski RS, Zhou J, Lee TDG, Hirsch GM, Powers KA, Khadaroo RG, Papia G, Kapus A, Rotstein OD, Furlan JC, Rosen IB, Stratford AFC, George RL, VanManen L, Klassen DR, Feldman LS, Mayrand S, Mercier L, Stanbridge D, Fried GM, Nanji SA, Hancock WW, Anderson C, Shapiro AMJ, Butter A, Martins L, Taylor B, Ott MC, Rycroft K, Wall WJ, Burpee SE, Schlachta CM, Mamazza J, Pace K, Poulin EC, Taylor MC, Christou NV, Jarand J, Sylvestre JL, McLean APH, Behzadi A, Tan L, Unruh H, Brandt MG, Darling GE, Miller L, Seely AJE, Maziak DE, Gunning D, Do MT, Bukhari M, Shamji FM, Abdurahman A, Darling G, Ginsberg R, Johnston M, Waddell T, Keshavjee S, Cuccarolo G, Charyk-Stewart T, Inaba K, Malthaner R, Gray D, Girotti M, Grondin SC, Tutton SM, Sichlau MJ, Pozdol C, McDonough TJ, Masters GA, Ray DW, and Liptay MJ
- Published
- 2002
12. Infection control in the operating room: current practices or sacred cows?
- Author
-
Lafrenière R, Bohnen JM, Pasieka J, and Spry CC
- Subjects
- Chicago, Congresses as Topic, Humans, Protective Clothing, Risk Factors, Cross Infection prevention & control, Infection Control methods, Operating Rooms
- Published
- 2001
- Full Text
- View/download PDF
13. Complications on a general surgery service: incidence and reporting.
- Author
-
Wanzel KR, Jamieson CG, and Bohnen JM
- Subjects
- Data Collection, Female, Hospitals, University, Humans, Incidence, Male, Medical Audit, Medical Errors adverse effects, Medical Errors mortality, Medical Records, Morbidity, Ontario epidemiology, Prospective Studies, Severity of Illness Index, Total Quality Management, Documentation statistics & numerical data, Iatrogenic Disease epidemiology, Intraoperative Complications epidemiology, Intraoperative Complications etiology, Medical Errors statistics & numerical data, Surgery Department, Hospital statistics & numerical data
- Abstract
Objectives: To determine the incidence and nature of complications on a general surgery service and to compare these results with pre-existing institutional recording and reporting methods., Design: A single observer prospectively monitored the presence and documentation of complications for all patients admitted to the general surgery service at the Wellesley Central Hospital over a 2-month period, through daily chart reviews, attendance at rounds and surgical operating rooms, frequent patient visits on the ward and interviews with the health care team., Setting: The general surgery service of an urban, university-affiliated teaching hospital., Patients: One hundred and ninety-two general surgery inpatients over 1277 patient-days from June 16, 1996, until Aug. 15, 1996. Same-day surgery patients were not included., Results: Seventy-five (39%) of the 192 patients suffered a total of 144 complications. Two complications (1%) were fatal, 10 (7%) were life threatening, 90 (63%) were of moderate severity and 42 (29%) were trivial. Of these 144 complications, 26 (18%) were deemed potentially attributable to error. One hundred and twelve (78%) of the complications occurred during or after a surgical operation and were related directly or indirectly to it. Only 9 (6%) complications were not documented in the progress notes of the patients' charts. However, 115 (80%) were not presented at weekly morbidity and mortality rounds, and 95 (66%) were not documented on the face sheet of the patients' final medical records., Conclusions: Complications are common and are underreported by traditional methods. Since hospital funding and quality improvement efforts depend on accurate identification and recording of adverse events, strategies to improve the recording and reporting of complications must be developed.
- Published
- 2000
14. Predicting infection in localized intraabdominal fluid collections: value of pH and pO2 measurements.
- Author
-
Wong JK, Mustard R, Gray RR, Sadler DJ, Sanabria J, Bohnen JM, Schouten BD, Doyle G, and Pugash RA
- Subjects
- Abdominal Abscess metabolism, Abdominal Abscess microbiology, Abdominal Abscess therapy, Adult, Aged, Aged, 80 and over, Drainage, Female, Forecasting, Humans, Hydrogen-Ion Concentration, Male, Middle Aged, Oxygen analysis, Paracentesis, Partial Pressure, Pleural Effusion metabolism, Pleural Effusion microbiology, Pleural Effusion therapy, Predictive Value of Tests, Prospective Studies, ROC Curve, Radiography, Interventional, Sensitivity and Specificity, Tomography, X-Ray Computed, Tumor Necrosis Factor-alpha analysis, Ultrasonography, Interventional, Abdominal Abscess diagnosis, Pleural Effusion diagnosis
- Abstract
Purpose: To evaluate the use of pH, pO2, and the subjective opinion of the radiologist compared with bacterial culture in accurate diagnoses of bacterial infection in intraabdominal fluid collections., Materials and Methods: Prospectively, 79 patients who were suspected of having an intraabdominal fluid collection underwent diagnostic fluid aspiration. The aspirate was cultured and measured for pH and pO2. A pH < or = 7.1 and a PO2 < or = 49 mm Hg were threshold values used to separate infected from sterile fluid collections., Results: pH alone had a 92% sensitivity and 79% specificity, whereas PO2 alone had a 51% sensitivity and 79% specificity. pH or pO2 combined yielded a 92% sensitivity and 60% specificity. The radiologist's opinion produced a 83% sensitivity and 92% specificity. pH and the radiologist's opinion combined produced a 78% sensitivity and 96% specificity. pH or the radiologist's opinion combined had a 95% sensitivity and a 63% specificity., Conclusion: pH is the most sensitive indicator of infection and the radiologist's opinion is the most specific. We recommend proceeding to drainage if the radiologist believes the collection to be infected and performing pH analysis if not. If the pH < or = 7.04, proceed to drainage. If neither of the above criteria are met, drainage could be delayed, pending the results of culture.
- Published
- 1999
- Full Text
- View/download PDF
15. The role of oral antimicrobials for the management of intra-abdominal infections.
- Author
-
Solomkin JS, Dellinger EP, Bohnen JM, and Rostein OD
- Subjects
- APACHE, Administration, Oral, Adult, Aged, Double-Blind Method, Female, Humans, Imipenem therapeutic use, Male, Middle Aged, Prospective Studies, Surgical Wound Infection microbiology, Abdomen microbiology, Anti-Infective Agents administration & dosage, Antitrichomonal Agents administration & dosage, Ciprofloxacin administration & dosage, Drug Therapy, Combination administration & dosage, Metronidazole administration & dosage, Surgical Wound Infection drug therapy
- Abstract
Background: Oral therapy for patients with complicated intra-abdominal infections has been very limited because those patients are frequently ill and need surgery. In addition, at the time of diagnosis and initial treatment, the infection is often accompanied by ileus, gastrointestinal tract function is frequently unknown, and many patients cannot tolerate oral intake. The use of oral antimicrobials in this setting is a recent advance resulting from the availability of agents with good tissue pharmacokinetics and potent aerobic gram-negative activity. This is the first prospective blinded study of oral therapy to provide data on the characteristics of patients eligible for oral treatment and the consequences of such treatment., Study Design: In blinded fashion, patients with complicated intra-abdominal infections were randomized to either i.v. ciprofloxacin plus metronidazole or i.v. imipenem throughout their treatment course, or i.v. ciprofloxacin plus metronidazole and treatment with oral ciprofloxacin plus metronidazole when oral feeding was resumed (CIP/MTZ i.v./oral). Physicians could switch the patient to oral therapy between 3 and 8 days after the start of i.v. treatment., Results: One hundred fifty-five of 330 (47%) patients were switched to active or placebo oral therapy. Patients who received i.v./oral therapy were treated, overall, for an average of 8.6 +/- 3.6 days, with an average of 4.0 +/- 3.0 days of oral treatment. Of 46 CIP/MTZ i.v./oral patients (active oral arm), treatment failure occurred in 2 patients (4%) compared with 41 patients (23%) who were not switched to oral agents. No patient or disease features, such as Acute Physiology and Chronic Health Evaluation II score, severity of illness at study entry, organ source of infection, or duration of treatment were identified as predictors of conversion to oral treatment., Conclusions: In this first prospective examination of sequential i.v./oral therapy for complicated intra-abdominal infections, conversion to oral therapy with ciprofloxacin plus metronidazole appears as effective as continued i.v. therapy for patients able to tolerate oral feedings. Patients who can tolerate oral intake may be treated with appropriate oral antimicrobials and are not at any significant increased risk for failure.
- Published
- 1998
16. Predicting the need for reoperation for abdominal infection.
- Author
-
Bohnen JM and Schouten BD
- Subjects
- Abdomen surgery, Humans, Reoperation, Reproducibility of Results, Risk Factors, Surgical Wound Infection epidemiology, Abdomen microbiology, Decision Trees, Severity of Illness Index, Surgical Wound Infection diagnosis, Surgical Wound Infection surgery
- Abstract
Abdominal infection complicating abdominal operation is a serious clinical problem that is subject to diagnostic delay, which is a risk factor for adverse outcomes. Clinical examination and laboratory and imaging modalities become more accurate at achieving a diagnosis once the patient becomes sicker from infection but cannot reliably predict the need for reoperation early in the postoperative course. The Abdominal Reoperation Predictive Index scoring system synthesizes common sense and objective measurements in an attempt to predict the need for reintervention before it is too late. We encourage other centers to test this predictor in their own patient populations.
- Published
- 1998
17. Antibiotic therapy for abdominal infection.
- Author
-
Bohnen JM
- Subjects
- Abdomen microbiology, Animals, Anti-Bacterial Agents pharmacology, Bacterial Infections microbiology, Clinical Trials as Topic, Disease Models, Animal, Drug Resistance, Microbial, Gastrointestinal Diseases drug therapy, Gastrointestinal Diseases microbiology, Humans, Peritonitis microbiology, Recurrence, Treatment Outcome, Anti-Bacterial Agents therapeutic use, Bacterial Infections drug therapy, Peritonitis drug therapy
- Abstract
Abdominal infections are treated by resuscitation, abdominal drainage, control of the source of infection, and antimicrobial agents. Ideally, antimicrobial therapy is active against expected pathogens, safe and effective in clinical trials, inexpensive, and unlikely to promote drug resistance. Numerous single-agent and combination-drug regimens have been efficacious in clinical trials, based on coverage of Escherichia coli and Bacteroides species, the predominant pathogens isolated. Whether expanded antimicrobial coverage is required, especially in hospital-acquired infections, is controversial. Candida infections should be treated with antifungal therapy in patients with recurrent abdominal infections, immunosuppressed patients, and those with candidal abscesses. Most agents have few serious adverse effects; aminoglycosides are the least expensive agents but cause nephro- and ototoxicity. There is little information on the promotion of drug resistance in this condition. Recent developments include the introduction of ticarcillin/clavulanic acid, ampicillin/ sulbactam, piperacillin/tazobactam, meropenem, aztreonam/clindamycin, and ciprofloxacin/metronidazole; success with once-daily aminoglycosides; evidence that antibiotics limit infectious complications of pancreatitis; controversy over the value of diagnostic cultures; the use of oral therapy; evidence in favor of shorter courses of treatment; and the introduction of pharmacoeconomic studies. Clinical investigators are challenged to improve drug trials by stratifying and controlling for the adequacy of surgical intervention.
- Published
- 1998
- Full Text
- View/download PDF
18. Soft tissue infections and the diabetic foot.
- Author
-
Smith AJ, Daniels T, and Bohnen JM
- Subjects
- Anti-Bacterial Agents therapeutic use, Combined Modality Therapy, Diabetic Foot complications, Diabetic Foot diagnosis, Drug Therapy, Combination therapeutic use, Humans, Soft Tissue Infections diagnosis, Soft Tissue Infections etiology, Diabetic Foot drug therapy, Diabetic Foot surgery, Soft Tissue Infections drug therapy, Soft Tissue Infections surgery
- Abstract
Soft tissue infections are classified as local or spreading. Spreading soft tissue infections are potentially life-threatening conditions, requiring prompt diagnosis and treatment. The information presented is based on a literature review and the authors' clinical experience. Diagnosis of soft tissue infections is aimed at determining the level of infection (skin, fascia, muscle) and whether necrosis is present. The bacteriology of these infections is varied and is of secondary importance. Treatment of skin infections that have no dead tissue is with antibiotics alone. Infections at the fascial or muscle level and those with necrosis at any level require surgical debridement and adjuvant antibiotics. The feet of diabetic patients are prone to plantar forefoot ulcers associated with tissue destruction and infection. The vast majority are caused by mechanical factors. If local immune defenses are adequate, bacterial colonization occurs without infection. Most diabetic foot ulcers will respond to relief of pressure, which may require total contact casting. Antibiotics and debridement are required in infected or deep ulcers, or when the ulcer does not respond to total contact casting.
- Published
- 1996
- Full Text
- View/download PDF
19. Surgical Infection Society position on vancomycin-resistant Enterococcus.
- Author
-
Davis JM, Huycke MM, Wells CL, Bohnen JM, Gadaleta D, Fichtl RE, and Barie PS
- Subjects
- Cross Infection prevention & control, Drug Resistance, Microbial, Humans, Vancomycin therapeutic use, Anti-Bacterial Agents pharmacology, Enterococcus drug effects, Gram-Positive Bacterial Infections prevention & control, Infection Control, Vancomycin pharmacology
- Abstract
The risk of transfer of vancomycin resistance to staphylococci is a real possibility and has been achieved in the laboratory. Prolonged colonization occurs with vancomycin-resistant Enterococcus (VRE), and many more patients are colonized than infected. The failure to identify, isolate, and adhere to infection control measures when caring for VRE-colonized patients dooms to failure any means to control its spread. Control of vancomycin use alone is unlikely to greatly affect the number of patients at risk for VRE colonization. The global spread of VRE may be impossible to stop, but infection control measures are the most important line of defense inside hospitals.
- Published
- 1996
- Full Text
- View/download PDF
20. Results of a randomized trial comparing sequential intravenous/oral treatment with ciprofloxacin plus metronidazole to imipenem/cilastatin for intra-abdominal infections. The Intra-Abdominal Infection Study Group.
- Author
-
Solomkin JS, Reinhart HH, Dellinger EP, Bohnen JM, Rotstein OD, Vogel SB, Simms HH, Hill CS, Bjornson HS, Haverstock DC, Coulter HO, and Echols RM
- Subjects
- Administration, Oral, Adolescent, Adult, Aged, Cilastatin therapeutic use, Cilastatin, Imipenem Drug Combination, Double-Blind Method, Drug Combinations, Humans, Imipenem therapeutic use, Infections microbiology, Infusions, Intravenous, Middle Aged, Treatment Outcome, Abdomen, Anti-Infective Agents therapeutic use, Ciprofloxacin therapeutic use, Drug Therapy, Combination therapeutic use, Infections drug therapy, Metronidazole therapeutic use
- Abstract
Objective: In a randomized, double-blind, multicenter trial, ciprofloxacin/metronidazole was compared with imipenem/cilastatin for treatment of complicated intra-abdominal infections. A secondary objective was to demonstrate the ability to switch responding patients from intravenous (IV) to oral (PO) therapy., Summary Background Data: Intra-abdominal infections result in substantial morbidity, mortality, and cost. Antimicrobial therapy often includes a 7- to 10-day intravenous course. The use of oral antimicrobials is a recent advance due to the availability of agents with good tissue pharmacokinetics and potent aerobic gram-negative activity., Methods: Patients were randomized to either ciprofloxacin plus metronidazole intravenously (CIP/MTZ IV) or imipenem intravenously (IMI IV) throughout their treatment course, or ciprofloxacin plus metronidazole intravenously and treatment with oral ciprofloxacin plus metronidazole when oral feeding was resumed (CIP/MTZ IV/PO)., Results: Among 671 patients who constituted the intent-to-treat population, overall success rates were as follows: 82% for the group treated with CIP/MTZ IV; 84% for the CIP/MTZ IV/PO group; and 82% for the IMI IV group. For 330 valid patients, treatment success occurred in 84% of patients treated with CIP/MTZ IV, 86% of those treated with CIP/MTZ IV/PO, and 81% of the patients treated with IMI IV. Analysis of microbiology in the 30 patients undergoing intervention after treatment failure suggested that persistence of gram-negative organisms was more common in the IMI IV-treated patients who subsequently failed. Of 46 CIP/MTZ IV/PO patients (active oral arm), treatment success occurred in 96%, compared with 89% for those treated with CIP/MTZ IV and 89% for those receiving IMI IV. Patients who received intravenous/oral therapy were treated, overall, for an average of 8.6 +/- 3.6 days, with an average of 4.0 +/- 3.0 days of oral treatment., Conclusions: These results demonstrate statistical equivalence between CIP/MTZ IV and IMI IV in both the intent-to-treat and valid populations. Conversion to oral therapy with CIP/MTZ appears as effective as continued intravenous therapy in patients able to tolerate oral feedings.
- Published
- 1996
- Full Text
- View/download PDF
21. Liposomal cefoxitin in a porcine model of intra-abdominal sepsis: hemodynamic changes.
- Author
-
Soltes S, Shek PN, Mustard RA, Soric I, Bohnen JM, and Mittelman MW
- Subjects
- Abdomen, Animals, Bacteremia physiopathology, Blood Pressure drug effects, Cardiac Output drug effects, Cefoxitin administration & dosage, Cefoxitin therapeutic use, Drug Carriers, Escherichia coli Infections physiopathology, Heart Rate drug effects, Liposomes, Male, Swine, Vascular Resistance drug effects, Bacteremia drug therapy, Cefoxitin pharmacology, Escherichia coli Infections drug therapy, Hemodynamics drug effects
- Abstract
The effects of free versus liposomal cefoxitin on various physiological parameters in a porcine model of Gram-negative intra-abdominal sepsis were evaluated. Four different doses of Escherichia coli inoculum mixed with sterile pig feces were used (10(8), 10(9), 10(10), and 10(11) cfu/animal), and the most consistent hemodynamic changes were observed with an inoculum of approximately 10(11) bacteria/20 kg animal. Two treatment groups were established as follows: free cefoxitin (n = 9) and liposomal cefoxitin (n = 9). All animals were maintained under anesthesia for the duration of the study, and then euthanized 24 h following intra-abdominal inoculation. The inoculated and nontreated animals showed increases in heart rate, mean pulmonary arterial pressure, systemic and pulmonary vascular resistance, and decreases in mean systemic arterial pressure and cardiac index. These changes were significant (p < .05) compared with a control group injected with normal saline. Liposomal cefoxitin-treated animals showed significantly lower decreases in mean systemic arterial pressure and increases in heart rate (p < .05) compared with both the inoculated nontreated and free cefoxitin-treated groups. Both liposomal and free cefoxitin significantly modulated the mean pulmonary arterial pressure compared with the inoculated nontreated animals (p < .05). Acidosis that developed during intra-abdominal infection diminished 6 h following the first dose of liposomal cefoxitin (p < .05). The results of these experiments demonstrate that liposomal cefoxitin exerts a beneficial modulation of some of the hemodynamic disturbances during intra-abdominal Gram-negative sepsis.
- Published
- 1996
22. Duration of antibiotic treatment in surgical infections of the abdomen. Postoperative peritonitis.
- Author
-
Bohnen JM
- Subjects
- Anti-Bacterial Agents therapeutic use, Humans, Postoperative Care, Time Factors, Peritonitis drug therapy, Postoperative Complications drug therapy
- Abstract
Postoperative peritonitis is potentially lethal and is usually caused by leakage of gut contents. Successful management depends on early diagnosis and treatment which require clinical suspicion and aggressive diagnostic imaging. Treatment consists of fluid and nutritional resuscitation, peritoneal toilet, control of gut leakage and initiation of antimicrobial therapy. Since delay in diagnosis is common, antimicrobial treatment is usually begun when the infection has become well developed. Experimental evidence has shown that in some settings antimicrobial agents do not perform as well in later stages of infection but whether this applies to peritonitis is not known. The optimal duration of antimicrobial therapy has not been studied specifically in postoperative peritonitis. Arguments for prolonged treatment include the potential for greater killing of bacteria in patients who have severe infections. Arguments against prolongation of therapy include the lesser role of antibiotics compared with operative management, doubt about the value of antibiotics' ability to kill bacteria at later stages of infection and the significant number of infective and non-infective complications of drug therapy. Limitation of antimicrobial treatment to no more than seven days is advocated. Persistent clinical signs, fever, or leucocytosis should prompt a search for a drainable focus of infection in the abdomen or treatable site elsewhere.
- Published
- 1996
23. Liposomal cefoxitin in a porcine model of intra-abdominal sepsis: bactericidal efficacy.
- Author
-
Soltes S, Mustard RA, Shek PN, Florio E, Bohnen JM, and Mittelman MW
- Subjects
- Analysis of Variance, Animals, Cefoxitin administration & dosage, Cephamycins administration & dosage, Disease Models, Animal, Escherichia coli isolation & purification, Liposomes, Male, Sepsis microbiology, Swine, Cefoxitin pharmacology, Cephamycins pharmacology, Escherichia coli drug effects, Sepsis drug therapy
- Abstract
The bactericidal effect of free versus liposomal cefoxitin was evaluated in the major reticuloendothelial organs in a porcine model of intra-abdominal sepsis. Yorkshire Landrace pigs were inoculated with 3.2 x 10(10) (n = 5) or 1.4 x 10(11) (n = 7) cfu of Escherichia coli mixed in sterile feces/animal. Two treatment groups inoculated with 1.4 x 10(11) cfu were established: free cefoxitin (n = 9) and liposomal cefoxitin (n = 9). All animals were maintained under anesthesia and euthanized after 24 h. The number of E. coli recovered in the liver, lungs, and spleen was significantly affected by inoculum size (p < .05). The liver had significantly higher numbers of bacteria (p < .05) compared with the other organs, regardless of the inoculum size. The liver and the lung of the liposomal cefoxitin-treated group showed significantly lower numbers of E. coli (5.0 x 10(4) and 6.3 x 10(2), respectively) compared with the untreated (liver, 6.3 x 10(7); lung, 2.0 x 10(6)) and free cefoxitin (liver, 5.0 x 10(6); lung, 7.9 x 10(4))-treated groups (p < .05). At 2 h following the injection of free and liposomal cefoxitin, the decrease of E. coli in peritoneal fluid compared with the nontreated septic group was significant (p < .05). No growth was observed from blood cultures taken 24 h after sepsis induction. All control experiments yielded negative cultures. The results of these experiments demonstrated that liposomal cefoxitin exerts an enhanced bactericidal effect in liver and lungs during Gram-negative sepsis.
- Published
- 1995
- Full Text
- View/download PDF
24. Definition of the role of enterococcus in intraabdominal infection: analysis of a prospective randomized trial.
- Author
-
Burnett RJ, Haverstock DC, Dellinger EP, Reinhart HH, Bohnen JM, Rotstein OD, Vogel SB, and Solomkin JS
- Subjects
- Abscess drug therapy, Adult, Anti-Infective Agents pharmacology, Anti-Infective Agents therapeutic use, Bacteremia drug therapy, Bacteremia microbiology, Ciprofloxacin pharmacology, Ciprofloxacin therapeutic use, Double-Blind Method, Drug Resistance, Microbial, Drug Therapy, Combination pharmacology, Enterococcus drug effects, Enterococcus isolation & purification, Female, Gram-Positive Bacterial Infections drug therapy, Gram-Positive Bacterial Infections mortality, Humans, Logistic Models, Male, Metronidazole pharmacology, Metronidazole therapeutic use, Middle Aged, Peritonitis drug therapy, Prospective Studies, Sepsis drug therapy, Sepsis microbiology, Sepsis mortality, Treatment Failure, Vancomycin pharmacology, Vancomycin therapeutic use, Abscess microbiology, Drug Therapy, Combination therapeutic use, Enterococcus pathogenicity, Gram-Positive Bacterial Infections microbiology, Peritonitis microbiology
- Abstract
Background: The role of enterococcus in intraabdominal infection is controversial. This study examines the contribution of enterococcus to adverse outcome in a large intraabdominal infection trial., Methods: A randomized prospective double-blind trial was performed to compare two different antimicrobial regimens in combination with surgical or percutaneous drainage in the treatment of complicated intraabdominal infections. A total of 330 valid patients was enrolled from 22 centers in North America., Results: In 330 valid patients, 71 had enterococcus isolated from the initial drainage of an intraabdominal focus of infection. This finding was associated with a significantly higher treatment failure rate than that of patients without enterococcus (28% versus 14%, p < 0.01). In addition, only Acute Physiology and Chronic Health Evaluation II score and presence of enterococcus were significant independent predictors of treatment failure when stepwise logistic regression was performed (p < 0.01 and < 0.03). Risk factors for the presence of enterococcus include age, Acute Physiology and Chronic Health Evaluation II, preinfection hospital length of stay, postoperative infections, and anatomic source of infection. There was no difference between the clinical trial treatment regimens with regard to overall failure, failure associated with enterococcus, or frequency of enterococcal isolation., Conclusions: This study is the first to report enterococcus as a predictor of treatment failure in complicated intraabdominal infections. This trial also identifies several significant risk factors for the presence of enterococcus in such infections.
- Published
- 1995
- Full Text
- View/download PDF
25. Surgical site surveillance: Quality improvement or waste of time?
- Author
-
Bohnen JM
- Published
- 1994
- Full Text
- View/download PDF
26. Deferoxamine induces hypotension in experimental gram-negative septicemia.
- Author
-
Mustard RA, Bohnen JM, Mullen JB, Schouten BD, and Swanson HT
- Subjects
- Animals, Deferoxamine pharmacokinetics, Disease Models, Animal, Gram-Negative Bacterial Infections complications, Gram-Negative Bacterial Infections mortality, Hemodynamics, Hypotension complications, Hypotension mortality, Lung physiopathology, Pseudomonas aeruginosa, Respiration Disorders physiopathology, Survival Rate, Swine, Deferoxamine pharmacology, Gram-Negative Bacterial Infections drug therapy, Hypotension chemically induced
- Abstract
Multiple organ system failure may result from tissue damage caused by activated neutrophils or endotoxin. A significant part of this tissue damage is due to peroxidation induced by oxygen-free radicals and requires iron as a co-factor. Iron chelation has been shown to prevent tissue damage in some models. This experiment was carried out to determine whether iron chelation with deferoxamine (DFO) would prevent lung damage in a swine model of Gram-negative septicemia. Fifteen animals were randomized to control, Pseudomonas aeruginosa infusion at a rate of 2 x 10(7) colony forming units/20 kg/min (septic group), or Pseudomonas infusion combined with DFO pretreatment at a dose of 80 mg/kg/h (septic-treated group). Three of six septic-treated animals became severely hypotensive and died during the course of the experiment as opposed to none of six septic animals. Surviving septic-treated animals were significantly hypotensive (60 +/- 24 mmHg mean arterial pressure) compared to septic (122 +/- 9 mmHg) and control (109 +/- 8 mmHg) animals. DFO did not improve respiratory function (e.g., pO2) or morphology in septic animals. We conclude that iron-chelation therapy with DFO at the above dosage results in a significant deterioration in cardiovascular function in septic swine. Lung damage was not prevented.
- Published
- 1994
- Full Text
- View/download PDF
27. Steroids, APACHE II score, and the outcome of abdominal infection.
- Author
-
Bohnen JM, Mustard RA, and Schouten BD
- Subjects
- Adult, Humans, Ontario, Prospective Studies, Abdomen, Glucocorticoids therapeutic use, Hospital Mortality, Infections mortality, Severity of Illness Index
- Abstract
Objective: To compare the outcome of abdominal infection in patients with or without previous systemic glucocorticoid therapy and to determine the effect of steroid administration on the relationship between APACHE II (Acute Physiology and Chronic Health Evaluation) scores and mortality., Hypothesis: Steroid therapy leads to greater mortality and relatively lower APACHE II scores., Design: Prospective cohort study., Setting: University hospital., Patients: Two hundred ninety-seven consecutive adult patients with abdominal infection treated by surgical or percutaneous drainage. Treatment was at the clinician's discretion. Seventy-one patients received systemic steroid therapy., Outcome Measures: APACHE II score, clinical course, and death in hospital; relationship between APACHE II score and mortality in the steroid and no steroid groups., Results: Thirty-three patients receiving steroid therapy (46%) died vs 55 (24%) of 226 patients not receiving steroid therapy. The APACHE II score (P < .0001) and steroid administration (P = .04) were each independently associated with death. Steroid-treated patients had the same probability of dying as "nonsteroid" patients with an APACHE II score a mean of 3.7 points higher (95% confidence limits, 0.03 and 7.7). The confidence that 2, 3, or 4 extra APACHE II points is the appropriate correction for steroid-treated patients is 80%, 60%, or 40%, respectively., Conclusions: Patients receiving steroid therapy appear to be at higher risk of dying of abdominal infection than predicted by APACHE II scores. The number of patients receiving cancer chemotherapy was too small to determine whether this was an additional risk factor. In the design of clinical trials stratified by APACHE II scores, steroid-treated patients should either be excluded or assigned two extra APACHE II points.
- Published
- 1994
- Full Text
- View/download PDF
28. Antimicrobial prophylaxis for surgical wounds. Guidelines for clinical care.
- Author
-
Page CP, Bohnen JM, Fletcher JR, McManus AT, Solomkin JS, and Wittmann DH
- Subjects
- Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents adverse effects, Humans, Infusions, Intravenous, Risk Factors, Societies, Medical, Surgical Wound Infection epidemiology, Surgical Wound Infection prevention & control, Anti-Bacterial Agents therapeutic use, Clinical Protocols standards, Premedication standards, Surgical Procedures, Operative standards, Surgical Wound Infection drug therapy
- Abstract
Prophylactic administration of antibiotics can decrease postoperative morbidity, shorten hospitalization, and reduce the overall costs attributable to infections. Principles of prophylaxis include providing effective levels of antibiotics in the decisive interval, and, in most instances, limiting the course to intraoperative coverage only. Use in The National Research Council clean contaminated operations is appropriate and, in many instances, has been proven beneficial. Antibiotic prophylaxis is also indicated for clean operations, such as those involved with insertion of prosthetic devices, that are associated with low infection risk and high morbidity. Extension of antibiotic prophylaxis to other categories of clean wounds should be limited to patients with two or more risk factors established by criteria in the study of the efficacy of nosocomial infection control (SENIC) because the baseline infection rate in these patients is high enough to justify their use. Cefazolin (or cefoxitin when anaerobic coverage is necessary) remains the mainstay of prophylactic therapy. Selection of an alternate agent should be based on specific contraindications, local infection control surveillance data, and the results of clinical trials. Newer criteria for determining the risk of "site infection" (wound and intracavitary) are in evolution and may lead to modification of these recommendations over the next several years.
- Published
- 1993
- Full Text
- View/download PDF
29. Operative management of intra-abdominal infections.
- Author
-
Bohnen JM
- Subjects
- Humans, Postoperative Care, Preoperative Care, Surgical Procedures, Operative methods, Abdomen surgery, Bacterial Infections surgery
- Abstract
Operation for intra-abdominal infection aims to prevent further contamination of the abdominal cavity, treat the underlying source of infection, and prevent residual or recurrent sepsis by cleaning the peritoneal cavity. Aggressive attempts at early diagnosis are warranted, even if laparotomy is occasionally required for diagnostic as well as therapeutic purposes. Conversely, the degree to which more aggressive methods of peritoneal debridement are helpful is the subject of great controversy that can be resolved only by prospective, randomized multicenter trials. Current standard treatment consists of closure, drainage, or excision of the source of contamination; intra-operative saline or antibiotic lavage of the peritoneal cavity; fascial closure; and secondary or delayed primary closure of the wound.
- Published
- 1992
30. Guidelines for clinical care: anti-infective agents for intra-abdominal infection. A Surgical Infection Society policy statement.
- Author
-
Bohnen JM, Solomkin JS, Dellinger EP, Bjornson HS, and Page CP
- Subjects
- Anti-Bacterial Agents administration & dosage, Appendicitis drug therapy, Drug Costs, Humans, Infections microbiology, Pancreatitis drug therapy, Peritonitis drug therapy, Abdomen, Anti-Bacterial Agents therapeutic use, Infections drug therapy
- Abstract
Several antibiotics have been marketed for therapeutic use in intra-abdominal infection. Often, these agents do not provide a sufficient spectrum activity against both facultative and obligate anaerobic gram-negative organisms, or have certain toxic effects that would not otherwise support their use. Guidelines have been developed for selection of antibiotic therapy for intra-abdominal infections and are presented as a statement of the Surgical Infection Society endorsed by the Executive Council. These guidelines are restricted to infections derived from the gastrointestinal tract and deal with those microorganisms commonly seen in such infections. The recommendations are based on in vitro activity against enteric bacteria, experience in animal models, and documented efficacy in clinical trials. Other concerns regarding pharmacokinetics, mechanisms of action, microbial resistance, and safety were also used in the formation of these guidelines. For community-acquired infections of mild to moderate severity, single-agent therapy with cefoxitin, cefotetan, or cefmetazole or ticarcillin-clavulanic acid is recommended. For more severe infections, single-agent therapy with carbapenems (imipenem/cilastatin) or combination therapy with either a third-generation cephalosporin, a monobactam (aztreonam), or an aminoglycoside plus clindamycin or metronidazole is recommended. Regimens with little or no activity against facultative gram-negative rods or anaerobic gram-negative rods are not considered acceptable.
- Published
- 1992
- Full Text
- View/download PDF
31. Antimicrobial prophylaxis in general surgery.
- Author
-
Bohnen JM
- Subjects
- Anti-Bacterial Agents administration & dosage, Humans, Anti-Bacterial Agents therapeutic use, Bacterial Infections prevention & control, Premedication, Surgical Procedures, Operative adverse effects
- Abstract
When used appropriately, antimicrobial prophylaxis is highly beneficial and cost effective. Antibiotics are not indicated for "clean procedures" such as hernia and breast surgery. A single preoperative dose will suffice, followed by an intraoperative dose if the operation takes more than 3 hours. For vascular (prosthesis or groin wound), head and neck (pharynx entered), thoracic (gastrointestinal or respiratory entrance) and high-risk gastroduodenal and biliary procedures, cefazolin, 1 g intravenously, is indicated. For procedures involving small intestine, appendix or penetrating abdominal trauma, cefoxitin or cefotetan, 2 g intravenously, is indicated. For colorectal procedures, either oral neomycin plus erythromycin or intravenous aminoglycoside plus clindamycin (or metronidazole) are effective. If valvular heart disease is present, endocarditis prophylaxis should be administered.
- Published
- 1991
32. Pneumonia complicating abdominal sepsis. An independent risk factor for mortality.
- Author
-
Mustard RA, Bohnen JM, Rosati C, and Schouten BD
- Subjects
- Adult, Aged, Aged, 80 and over, Bacterial Infections surgery, Cause of Death, Cross Infection mortality, Female, Humans, Male, Middle Aged, Multiple Organ Failure complications, Multiple Organ Failure mortality, Peritonitis complications, Peritonitis mortality, Peritonitis surgery, Pneumonia mortality, Prospective Studies, Recurrence, Risk Factors, Severity of Illness Index, Shock, Septic complications, Shock, Septic mortality, Suppuration, Abdomen, Bacterial Infections complications, Cross Infection complications, Pneumonia complications
- Abstract
Nosocomial pneumonia (NP) is associated with a significant mortality, 66% in a previous retrospective study of NP complicating intra-abdominal sepsis (IAS). We prospectively compared the outcome of NP complicating IAS with that of recurrent IAS (R-IAS) in the absence of NP. Data were collected prospectively on 300 patients with IAS; 34 patients who presented with pneumonia were excluded from the analysis (44% mortality). One hundred seventy-one patients with no NP and no R-IAS (group 1) had a hospital mortality of 20% (34 patients); 36 without NP in whom R-IAS developed (group 2) had a 17% mortality (six patients); and 47 with NP but no R-IAS (group 3) had a 53% mortality (25 patients). Finally, 12 patients who had both NP and R-IAS suffered a 75% mortality (nine patients). We examined the relationships among the following putative risk factors and mortality: APACHE (acute physiology and chronic health evaluation) II score (at initial presentation with IAS), the need for mechanical ventilatory assistance following initial treatment for peritonitis, steroid requirement, generalized peritonitis vs abscess, and the need for surgical as opposed to percutaneous treatment. Using mortality as the dependent variable, group 2 vs 3 as the explanatory variable, and the risk factors as confounders, logistic regression analysis indicated that the group difference was significant after controlling for confounders. We conclude that NP complicating IAS is an independent risk factor associated with a significant mortality compared with R-IAS. These data challenge the notion that death in IAS is usually due to recurrent or persistent intra-abdominal infection.
- Published
- 1991
- Full Text
- View/download PDF
33. A critical look at scheduled relaparotomy for secondary bacterial peritonitis.
- Author
-
Bohnen JM and Mustard RA
- Subjects
- Clinical Trials as Topic, Evaluation Studies as Topic, Humans, Peritonitis etiology, Reoperation, Bacterial Infections surgery, Laparotomy methods, Peritonitis surgery
- Abstract
Scheduled relaparotomy is attracting interest as a means of treating intra-abdominal sepsis. It is the subject of several studies to evaluate its role in the management of peritoneal infection. However, indications for this procedure are not clear and current knowledge does not allow for adequate scientific evaluation. Many trials lack control groups and historical control groups cannot be evaluated on criteria, such as illness severity, concomitant health problems or even surgical technique. Indicators of outcome are generally described in insufficient detail; only death or survival are reported and important morbidity and resource utilization data are not included. A randomized, prospective, multicenter clinical trial using standardized techniques is necessary to determine the usefulness of scheduled relaparotomy performed on different patients.
- Published
- 1991
34. Campylobacter pyloridis is associated with acid-peptic disease in Toronto.
- Author
-
Bohnen JM, Krajden S, Anderson JG, Kempston JD, Fuksa M, Karmali MA, Osborne A, and Babida C
- Subjects
- Adolescent, Adult, Aged, Campylobacter isolation & purification, Female, Gastritis diagnosis, Gastritis microbiology, Gastroscopy, Humans, Male, Middle Aged, Peptic Ulcer diagnosis, Peptic Ulcer etiology, Peptic Ulcer microbiology, Pilot Projects, Campylobacter Infections microbiology, Gastritis etiology
- Abstract
Campylobacter pyloridis has been associated with acid-peptic disease in centres outside Canada. The authors conducted a pilot study to see if this association existed in Toronto. Patients in whom esophagogastroscopy was indicated on clinical grounds were arbitrarily selected for determination of the presence of C. pyloridis. Included in the study were 100 patients who underwent 105 endoscopies. In 75 patients (80 endoscopies) there was some form of acid-peptic disease (inflammation or ulceration of stomach or duodenum). Of those with acid-peptic disease, 34% had C. pyloridis compared with 4% among patients without acid-peptic disease (p less than 0.01). Men with acid-peptic disease were more likely to harbour C. pyloridis than women (48% versus 16%, p less than 0.01). The organisms were curved gram-negative rods that appeared as small colonies after 4 days of incubation under microaerobic conditions. They were strongly urease positive. There was considerable heterogeneity of endoscopic diagnoses. Future clinical studies of C. pyloridis need careful endoscopic and histologic classification.
- Published
- 1986
35. Treatment of intra-abdominal sepsis.
- Author
-
Bohnen JM and Meakins JL
- Subjects
- Abscess diagnosis, Abscess surgery, Anti-Bacterial Agents therapeutic use, Drainage, General Surgery, Humans, Peritoneal Cavity, Peritonitis diagnosis, Peritonitis surgery, Therapeutic Irrigation, Abdomen surgery, Abscess therapy, Peritonitis therapy
- Abstract
Twenty-three surgeons at three McGill University hospitals were interviewed about their treatment of intra-abdominal sepsis. They described their use of antibiotics, operative practices and other treatment of generalized peritonitis and intra-abdominal abscesses. If more than 75% of respondents used a given method, its use was considered "uniform" unless substantial interhospital variation existed for that method. Treatment was variable in 18 situations. Only four of these involved systemic antibiotic use--drug regimens in appendicitis and intra-abdominal abscess, and duration of antibiotic therapy following appendicitis and perforated duodenal ulcer. The other 14 examples of variation were in operative management. In generalized peritonitis, they were: use of diagnostic paracentesis; abdominal lavage with saline alone versus saline plus antibiotic use; whether the peritoneum should ever be left open; the use or avoidance of drains; primary versus delayed wound closure in appendicitis, bowel perforation and trauma with gastrointestinal perforation and, finally, wound lavage with saline alone or with antibiotics. Treatment of intra-abdominal abscesses varied in regard to the diagnostic and therapeutic roles of percutaneous needle aspiration, the preferred route of drainage of a pelvic abscess, the use of an extra- or trans-serosal approach to a subphrenic abscess, local versus full abdominal exploration for a single abscess and the type of drain used. The authors conclude that operative management of intra-abdominal sepsis varies widely among surgeons. This fact invalidates many "controlled" trials of antibiotics and should focus attention less on drugs and more on surgical treatment.
- Published
- 1984
36. Anergy secondary to sepsis in rats. Relation to outcome.
- Author
-
Bohnen JM, Christou NV, Chiasson L, DeVoe IW, and Meakins JL
- Subjects
- Abscess etiology, Animals, Disease Models, Animal, Male, Peritonitis diagnosis, Peritonitis immunology, Prognosis, Rats, Rats, Inbred Strains, Skin Tests, Bacterial Infections immunology, Hypersensitivity, Delayed immunology
- Abstract
A rat model was developed to determine if delayed hypersensitivity skin tests during early peritonitis would predict outcome. Presensitized rats were simultaneously tested intradermally with keyhole-limpet hemocyanin and given four types of fecal bacteria and 10% barium sulfate intraperitoneally. Rats were divided into four groups according to inoculum dose. Skin tests were read at 24 hours in survivors and correlated with death and abscesses during the next 19 days. In the two groups with greatest mortality, 35% of anergic rats died, compared with 0% of reactive rats. In the three groups with most abscesses, anergic rats had more abscesses than those that reacted. Overall, 90% of anergic rats died or had abscesses v only 10% of reactors. We concluded that the outcome of untreated peritonitis is determined in the first 24 hours; anergy at that time predicts death or abscess formation.
- Published
- 1984
- Full Text
- View/download PDF
37. Antibiotic efficacy in intraabdominal sepsis: a clinically relevant model.
- Author
-
Bohnen JM, Matlow AG, Mustard RA, Christie NA, and Kavouris B
- Subjects
- Animals, Disease Models, Animal, Male, Random Allocation, Rats, Rats, Inbred Strains, Bacterial Infections drug therapy, Cefoxitin therapeutic use, Peritonitis drug therapy
- Abstract
We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excision and peritoneal lavage, 18 h after the onset of infection. This is different from previous models of abdominal sepsis (in which antibiotics are given within 4 h of peritoneal contamination) and mimics the clinical setting in which antibiotics are initiated much later, at the time of operation. Antibiotic-treated rats received 20 mg cefoxitin i.m. every 8 h for 7 days; controls received saline at similar times. Thirty-nine of 67 control rats died (58%) versus 20 of 64 (31%) that received cefoxitin (p less than 0.005). We conclude that even with delayed administration, antibiotics appear to improve the outcome of intraabdominal sepsis. With further characterization of this model we plan to use it as an in vivo assay to compare the efficacy of different antimicrobial agents in intraabdominal sepsis.
- Published
- 1988
- Full Text
- View/download PDF
38. Pseudomembranous colitis and wound infection following perioperative use of multiple antibiotics.
- Author
-
Bohnen JM, Matlow AG, and Cohen MM
- Subjects
- Aged, Clostridium Infections chemically induced, Colectomy adverse effects, Drug Therapy, Combination adverse effects, Female, Humans, Preoperative Care, Surgical Wound Infection microbiology, Anti-Bacterial Agents adverse effects, Enterocolitis, Pseudomembranous chemically induced, Surgical Wound Infection chemically induced
- Abstract
The prophylactic use of antibiotics in elective surgery of the colon is accepted practice, but it has inherent risks. The authors report the case of a 70-year-old woman who had wound infection and severe, relapsing pseudomembranous colitis due to Clostridium difficile after a short course of antibiotics given orally and parenterally at the time of elective resection of the colon. Perioperatively, she received erythromycin base and neomycin orally, plus netilmicin and metronidazole intravenously. Although the concomitant administration of parenteral antibiotics may enhance the benefit of antibiotics given orally before operation, this does not entirely prevent wound infection. Until the relation between the number of drugs and risk of antibiotic-associated colitis is more clearly defined, caution should be exercised in the use of multiple antibiotics in elective colonic surgery.
- Published
- 1985
39. Predicting postoperative complications.
- Author
-
Bohnen JM, Christou NV, Maclean LD, and Meakins JL
- Subjects
- Humans, Hypersensitivity, Delayed diagnosis, Skin Tests, Postoperative Complications diagnosis
- Published
- 1983
40. Common pancreaticobiliary channels and their relationship to gallstone size in gallstone pancreatitis.
- Author
-
Jones BA, Salsberg BB, Mehta MH, and Bohnen JM
- Subjects
- Cholangiography, Cholelithiasis pathology, Gallstones complications, Gallstones pathology, Humans, Cholelithiasis complications, Common Bile Duct pathology, Pancreatic Ducts pathology, Pancreatitis etiology
- Abstract
Bile reflux into the pancreatic duct after impaction of a stone in a common pancreaticobiliary channel has been suggested to be the initiating factor in gallstone pancreatitis. Such reflux would require that the impacted stone be smaller than the length of the common channel. The incidence of common channels was studied and gallstone size was compared with common channel length in patients with gallstone pancreatitis and those with cholelithiasis or choledocholithiasis without pancreatitis. Sixty-seven per cent of patients with gallstone pancreatitis had a common channel present on intraoperative cholangiography versus 32% of patients with cholelithiasis or choledocholithiasis without pancreatitis (p less than 0.005). Common channel length was greater than the diameter of the smallest stone in nine of 27 patients with gallstone pancreatitis and in 13 of 109 patients with cholelithiasis or choledocholithiasis without pancreatitis (p less than 0.025). In conclusion, common channels are more frequent in patients with gallstone pancreatitis than in patients with other biliary tract disease. Furthermore, gallstone pancreatitis is associated with stones that are smaller than the common channel, which favors obstruction of both pancreatic and bile ducts while allowing reflux of contents between them.
- Published
- 1987
- Full Text
- View/download PDF
41. C-reactive protein levels predict postoperative septic complications.
- Author
-
Mustard RA Jr, Bohnen JM, Haseeb S, and Kasina R
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Laparotomy adverse effects, Male, Middle Aged, Probability, Prospective Studies, Bacterial Infections blood, C-Reactive Protein analysis, Postoperative Complications blood
- Abstract
We studied 108 patients undergoing clean-contaminated and dirty surgical procedures to determine whether daily C-reactive protein (CRP) measurements for 14 days postoperatively could predict the occurrence of septic complications prior to clinical diagnosis. Diagnostic criteria for septic complications and positive CRP response were defined in advance of the study. The CRP assays were carried out using an automated laser nephelometer system after the patient's discharge from the hospital. Forty-six septic complications were diagnosed in 40 patients. These complications consisted of wound infection (23), urinary tract infection (11), pneumonia (six), upper respiratory tract infection (three), intra-abdominal abscess (one), and other (two). The CRP testing was found to have a positive predictive value of 69% and a negative predictive value of 78%. We conclude that serial CRP measurements may be a valuable adjunct to surgical care in patients at high risk of postoperative septic complications.
- Published
- 1987
- Full Text
- View/download PDF
42. Suppression of delayed cutaneous hypersensitivity and inflammatory cell delivery by sterile barium peritonitis.
- Author
-
Bohnen JM, Christou NV, and Meakins JL
- Subjects
- Animals, Bacterial Infections immunology, Male, Rats, Barium Sulfate, Dermatitis, Contact immunology, Peritonitis immunology, Phagocytes immunology
- Abstract
We have previously shown that experimental peritonitis secondary to fecal bacteria plus barium sulfate suppresses delayed cutaneous hypersensitivity (DCH) in rats. We examined herein the role of barium sulfate. In a series of experiments presensitized rats were simultaneously skin tested with intradermal keyhole limpet hemocyanin and given an intraperitoneal injection of either (1) a mixture of four fecal bacteria in their nutrient broths, (2) bacteria and broths plus barium sulfate, (3) sterile broths plus barium, (4) sterile barium alone, (5) nutrient broths, or (6) saline. In rats given sterile barium we measured phagocyte delivery to subcutaneous polyvinyl alcohol sponges. We found that (1) the coadministration of barium sulfate was necessary for rats given bacteria to die (P = 0.03) or develop abdominal abscesses (P less than 0.005), (2) suppression of DCH occurred in 70% of rats receiving sterile barium sulfate vs 0% in saline controls (P = 0.0001), (3) early suppression of DCH was associated with subsequent death and abscess formation in rats given bacteria plus barium (P = 0.00002) and with intraabdominal barium collections in rats given barium alone (P less than 0.02), (4) barium sulfate administration caused suppression of phagocyte delivery to subcutaneous sponges: 23.2 X 10(6) cells/site vs 43.1 X 10(6) cells/site in saline controls (P less than 0.005). We conclude that barium sulfate itself has profound systemic effects in the rat model of intraabdominal sepsis. Early suppression of DCH is associated with a poor outcome in septic rats.
- Published
- 1987
- Full Text
- View/download PDF
43. APACHE II score and abdominal sepsis. A prospective study.
- Author
-
Bohnen JM, Mustard RA, Oxholm SE, and Schouten BD
- Subjects
- Female, Hospitalization, Humans, Information Systems, Male, Middle Aged, Ontario, Outcome and Process Assessment, Health Care, Prospective Studies, Risk Factors, Abscess classification, Bacterial Infections classification, Diagnosis-Related Groups, Peritonitis classification, Severity of Illness Index
- Abstract
Therapeutic trials for intra-abdominal sepsis require pretreatment stratification; physiologic scoring has been recently proposed for this purpose. We have prospectively tested the validity of one such scoring system, namely, the Acute Physiology and Chronic Health Evaluation (APACHE II). As part of a larger database, we correlated APACHE II scores with mortality in 100 patients hospitalized for generalized peritonitis or abdominal abscess. Use of steroids was recorded because of our suspicion that steroids increase mortality but blunt the physiologic response to sepsis. Thirty-one patients died, including 12 of 19 patients receiving steroids. Stepwise discriminant analysis revealed that the APACHE II score and steroid use were each independently associated with the rate of mortality. We report a prospective validation of pretreatment APACHE II scoring in abdominal sepsis. Steroid use is an independent risk factor.
- Published
- 1988
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.