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1. A positive control mutant of the transcription activator protein FIS

Author

Gosink, Khoosheh K., Gaal, Tamas, Bokal, Anton J., IV, and Gourse, Richard L.

Subjects
Escherichia coli -- Genetic aspects, Microbial mutation -- Analysis, DNA binding proteins -- Genetic aspects, Genetic transcription -- Physiological aspects, Biological sciences
Abstract

Positive control mutants of the 98-amino acid DNA-binding FIS protein was detected in Escherichia coli. The control mutant of fis (fispc) exhibit single substitutions on the 71, 72 and 73 amino acid positions that causes defective transcription activation. Analysis of the substitution in position 72 indicated guanine-to-serine mutation that has not affected the DNA binding and bending characteristics of the positive control mutant.

Published
1996

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