1. The dry powder formulation of mixed cross-linked dextran microspheres and tetanus toxoid-loaded trimethyl chitosan nanospheres as a potent adjuvant for nasal delivery system
- Author
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Kabiri, Mona, Bolourian, Haleh, Dehghan, Solmaz, and Tafaghodi, Mohsen
- Subjects
Trimethyl chitosan - nanoparticles ,Systemic responses ,cross ,Tetanus toxoid ,Mucosal immunity ,linked dextran microspheres mucosal immunity nasal immunization systemic responses tetanus toxoid trimethyl chitosan nanoparticles ,Short Communication ,Cross-linked dextran – microspheres ,lcsh:R ,lcsh:Medicine ,Nasal immunization - Abstract
Objective(s): The present study aimed to determine the immunoadjuvant efficacy of mixed cross-linked dextran microspheres (CDM) and tetanus toxoid (TT)-loaded trimethyl chitosan (TMC) nanospheres in dry powder form. Materials and Methods: The TMC nanoparticles (NPs) containing TT were produced using the ionic gelation method. Co-administration of TT-loaded TMC NPs and CDM as an absorption enhancer was performed to improve immunity against the antigen. Dry powder formulations were delivered via the nasal route in a rabbit model. Results: Among immunization groups, mixing of CDM with TT encapsulated in TMC NPs could elicit the highest titer of systemic IgG antibody. Furthermore, the addition of CDM to TT-loaded TMC enhanced the sIgA response relative to the TT solution. Conclusion: The TMC NPs had a considerable effect on mucosal and systemic immunity against the TT antigen. Therefore, the CDM excipient can be utilized for nasal immunization to elevate systemic and mucosal responses.
- Published
- 2021