53 results on '"Borawska MH"'
Search Results
2. Polish and New Zealand Propolis as Sources of Antioxidant Compounds Inhibit Glioblastoma (T98G, LN-18) Cell Lines and Astrocytoma Cells Derived from Patient.
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Moskwa J, Naliwajko SK, Markiewicz-Żukowska R, Gromkowska-Kępka KJ, Soroczyńska J, Puścion-Jakubik A, Borawska MH, Isidorov V, and Socha K
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Gliomas, including glioblastoma multiforme and astrocytoma, are common brain cancers in adults. Propolis is a natural product containing many active ingredients. The aim of this study was to compare the chemical composition, total phenolic content and concentration of toxic elements as well as the anticancer potential of Polish (PPE) and New Zealand (Manuka-MPE) propolis extracts on diffuse astrocytoma derived from patient (DASC) and glioblastoma (T98G, LN-18) cell lines. The antioxidants such as flavonoids and chalcones (pinocembrin, pinobanksin, pinobanksin 3-acetate and chrysin) were the main components in both types of propolis. The content of arsenic (As) and lead (Pb) in MPE was higher than PPE. The anti-proliferative study showed strong activity of PPE and MPE propolis on DASC, T98G, and LN-18 cells by apoptosis induction, cell cycle arrest and attenuated migration. These findings suggest that despite their different geographic origins, Polish and New Zealand propolis are sources of antioxidant compounds and show similar activity and a promising anti-glioma potential in in vitro study. However, further in vivo studies are required in order to assess therapeutic potential of propolis.
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- 2022
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3. Association of allelic combinations in selenoprotein and redox related genes with markers of lipid metabolism and oxidative stress - multimarkers analysis in a cross-sectional study.
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Tarhonska K, Raimondi S, Specchia C, Wieczorek E, Reszka E, Krol MB, Gromadzinska J, Wasowicz W, Socha K, Borawska MH, and Jablonska E
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- Alleles, Biomarkers, Cholesterol, LDL, Cross-Sectional Studies, Glutathione Peroxidase genetics, Glutathione Peroxidase metabolism, Humans, Lipid Peroxidation, Oxidative Stress genetics, Selenoproteins genetics, Selenoproteins metabolism, Triglycerides, Lipid Metabolism genetics, Selenium
- Abstract
Background: Selenium (Se) and selenoproteins have been shown to be involved in lipid metabolism mainly due to their ability to modulate redox homeostasis in adipose tissue. The underlying mechanisms are yet to be evaluated. In the light of few data related to the association between polymorphic variants of selenoprotein encoding genes and metabolic syndrome or obesity in humans, the role of selenoprotein polymorphisms in lipid metabolism remains unclear. The aim of this study was to investigate the impact of allelic combination within selenoprotein and redox related genes on the markers of lipid metabolism and oxidative stress., Methods: The study comprised 441 healthy individuals from Poland, in the 18-74 year age group. Allelic combinations were investigated within the polymorphic variants of four selenoprotein encoding genes (GPX1 rs1050450, GPX4 rs713041, SELENOP rs3877899 and SELENOF rs5859) and the redox related gene (SOD2 rs4880). The impact of the most common allelic GPX1-GPX4-SELENOP-SELENOF-SOD2 combinations was assessed on the following markers: triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), glutathione peroxidase activities (GPX1, GPX3), lipid peroxidation (as TBARS), ceruloplasmin (Cp) and superoxide dismutase 1 (SOD1)., Results: Multivariable analysis revealed significant associations between three allelic combinations and markers of lipid metabolism, including HDL-C and TC/HDL-C ratio (AAAAa), LDL-C (aaAaa), and triglycerides (aaaaA), whereas two allelic combinations (aAaAA, aaaAA) were associated with GPX3 activity., Conclusion: This study confirms the possible implication of selenoproteins in lipid metabolism and warrants further research on specific allele combinations within selenoprotein and redox related genes in order to identify functional genetic combinations linked to metabolic phenotype., (Copyright © 2021 The Authors. Published by Elsevier GmbH.. All rights reserved.)
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- 2022
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4. Estimation of Selected Minerals in Aortic Aneurysms-Impaired Ratio of Zinc to Lead May Predispose?
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Socha K, Karwowska A, Kurianiuk A, Markiewicz-Żukowska R, Guzowski A, Gacko M, Hirnle T, and Borawska MH
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- Copper, Humans, Lead, Zinc, Aortic Aneurysm, Abdominal, Aortic Aneurysm, Thoracic, Selenium
- Abstract
The objective of this study was to estimate the content of copper, zinc, selenium, cadmium, and lead in the tissue of patients with aortic aneurysms. Molar ratio of Cu/Zn and antioxidant micronutrients to toxic elements was also calculated. A total of 108 patients: 47 with abdominal aortic aneurysm (AAA), 61 patients with thoracic aortic aneurysm (TAA), and a control group of 20 abdominal aortic (AA) and 20 thoracic aortic (TA) wall samples from the deceased were studied. The concentrations of mineral components in the tissue samples were determined by the AAS method. The average concentration of Cu in the aortic wall of patients with TAA was significantly lower than in the aortic wall samples of healthy people. The mean concentration of Zn in the aortic wall of patients with AAA and TAA was significantly lower than in the control group samples. Cu/Zn ratio was significantly higher in AAA patients than in control group which indicates a greater role of oxidative stress and inflammatory process in this type of aneurysm. The concentration of Se was significantly decreased in TAA patients compared with the control group; in turn, the concentration of Pb was increased in this group of patients. We observed significantly lower Cu/Pb ratio in TAA patients than in control group, whereas Zn/Pb ratio was significantly lower comparing with control samples in both types of aneurysms. In the examined aneurysms, we have shown the differences in concentrations of mineral components compared with the control tissues. The Zn concentration was decreased in both AAA and TAA samples. Impaired ratio of Zn to Pb may predispose to aortic aneurysms.
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- 2021
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5. Cadmium, lead and mercury in the blood of psoriatic and vitiligo patients and their possible associations with dietary habits.
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Wacewicz-Muczyńska M, Socha K, Soroczyńska J, Niczyporuk M, and Borawska MH
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- Cadmium, Case-Control Studies, Feeding Behavior, Female, Humans, Lead, Male, Mercury, Vitiligo epidemiology
- Abstract
Background: Psoriasis and vitiligo are common, autoimmune skin diseases, their etiology is still unclear. The relationship between environmental factors including diet and various skin diseases has been studied. The general mechanism of cadmium (Cd), lead (Pb) and mercury (Hg) toxicity is through the production of reactive oxygen species which are known to play a role in etiopathogenesis of skin disorders., Objective: The aim of this study was to estimate the influence of dietary habits on the concentration of Cd, Pb and Hg in the peripheral blood samples of patients with psoriasis and vitiligo., Methods: In this case-control study, sixty patients with psoriasis, fifty patients with vitiligo and fifty eight healthy people were examined. Blood levels of Cd, Pb and Hg were determined by atomic absorption spectrometry. Food-frequency questionnaires were implemented to collect the dietary data., Results: Significant differences (p < 0.05) of Cd levels were found between women and men with psoriasis and women and men in the control group. The concentration of Pb was significantly higher among vitiligo patients (50.04 ± 26.54 μg/L) than in healthy controls (36.04 ± 27.35 μg/L). Significantly lower ratio of Se/Pb, Zn/Pb and Cu/Pb was found among psoriatic men. Significantly (p < 0.05) lower values of Se/Hg ratio were observed among vitiligo patients compared to controls., Conclusions: The elevated levels of toxic elements could increase oxidative stress which may partly contribute to inflammation in the pathogenesis of psoriasis and vitiligo, which requires further research. Analysis of the influence of frequent consumption of food products on toxic metals concentration showed that the dietary habits have impact on the content of examined toxic metals in the blood of patients. The obtained results may be useful for composing the diet and could be helpful in prevention of psoriasis and vitiligo., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)
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- 2021
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6. Proximal Composition and Nutritive Value of Raw, Smoked and Pickled Freshwater Fish.
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Mielcarek K, Puścion-Jakubik A, Gromkowska-Kępka KJ, Soroczyńska J, Naliwajko SK, Markiewicz-Żukowska R, Moskwa J, Nowakowski P, Borawska MH, and Socha K
- Abstract
The aim of the study was to assess protein, fat, salt, collagen, moisture content and energy value of freshwater fish purchased in Polish fish farms. Eight species of freshwater fish (raw, smoked, pickled) were assessed by near infrared spectroscopy (NIRS). The protein content varied between 15.9 and 21.7 g/100 g, 12.8 and 26.2 g/100 g, 11.5 and 21.9 g/100 g in raw, smoked and pickled fish, respectively. Fat content ranged from 0.89 to 22.3 g/100 g, 0.72 to 43.1 g/100 g, 0.01 to 29.7 g/100 g in raw, smoked and pickled fish, respectively. Salt content ranged from 0.73 to 1.48 g/100 g, 0.77 to 3.39 g/100 g, 1.47 to 2.29 g/100 g in raw, smoked and pickled fish, respectively. A serving (150 g) of each fish product provided 53.2-71.9% of the Reference Intake (RI) for protein, 2.21-60.3% of the RI for fat, 21.3-61.3% of the RI for salt and 6.27-24.4% kJ/6.29-24.5% kcal of the RI for energy. Smoked fish had a higher protein and also fat content than raw and pickled fish, while smoked and pickled fish had higher salt content than raw fish. Cluster analysis was performed, which allowed to distinguish, on the basis of protein, fat, salt, collagen and moisture content, mainly European eel.
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- 2020
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7. Chemical composition of Polish propolis and its antiproliferative effect in combination with Bacopa monnieri on glioblastoma cell lines.
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Moskwa J, Naliwajko SK, Markiewicz-Żukowska R, Gromkowska-Kępka KJ, Nowakowski P, Strawa JW, Borawska MH, Tomczyk M, and Socha K
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- Cell Line, Tumor, Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry, Humans, Propolis pharmacology, Bacopa chemistry, Brain Neoplasms pathology, Cell Proliferation drug effects, Glioblastoma pathology, Plant Extracts pharmacology, Propolis chemistry
- Abstract
Propolis and Bacopa monnieri (L.) Wettst. (Brahmi) are natural products that contain many active substances and possess anticancer properties. The aim of this study was to investigate the chemical composition of Polish propolis extract (PPE) by gas chromatography-mass spectrometry (GC-MS), B. monnieri extracts (BcH, BcS) by high performance liquid chromatography with diode array detector and mass spectrometry coupled with electrospray ionization (LC-ESI-MS) and finally determine its anti-proliferative potential combined with BcH and BcS in glioblastoma cell lines (T98G, LN-18, U87MG). The antiproliferative activity of PPE, BcH, BcS and their combination (PPE + BcH) was determined by a cytotoxicity test, and DNA binding was determined by [
3 H]-thymidine incorporation. Flavonoids and phenylpropenoids were the main components of PPE. BcH and BcS samples were also successfully analyzed. Their main constituents were saponins such as bacoside A3, bacopaside II, X and bacopasaponin C and its isomer. The inhibitory effects on the viability and proliferation of the tested glioma cells observed after incubation with the combination of PPE and BcH were significantly stronger than the effects of these two extracts separately. These findings suggest that propolis in combination with B. monnieri shows promising anticancer activity for the treatment of glioblastoma. However, further studies are still required.- Published
- 2020
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8. The two faces of Coprinus comatus-Functional properties and potential hazards.
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Nowakowski P, Naliwajko SK, Markiewicz-Żukowska R, Borawska MH, and Socha K
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- Humans, Agaricales chemistry, Coprinus chemistry
- Abstract
Mushrooms have been used for centuries not only as food but also in traditional medicine as a source of components with pro-health activity. One of them is Coprinus comatus (O.F.Müll.) Pers. also called shaggy mane, chicken drumstick mushroom, or lawyer's wig. In Asian countries, C. comatus (CC) is approved as edible mushroom and often cultivated for consumption, whereas in many other countries, although it is widespread, it is unrecognized and not used. In this review, for the first time, we discussed about the composition related to functional properties as well as the potential risks associated with consumption of CC by reviewing scientific literature. The information has been collected in order to get to know this species thoroughly. Various studies show many of the physiological activities, such as antioxidant, anticancer, antiandrogenic, hepatoprotective, acetylcholinesterase inhibitory, antiinflammatory, antidiabetic, antiobesity, antibacterial, antifungal, antinematode, and antiviral. Besides positive physiological properties, CC has also negative features, for example, skin reactions in patients with dermatitis and atopic predisposition, risk of confusion with poisonous mushrooms, quick autolysis after collection, and contamination of toxic elements., (© 2020 The Authors. Phytotherapy Research published by John Wiley & Sons Ltd.)
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- 2020
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9. Comparison of Zinc, Copper and Selenium Content in Raw, Smoked and Pickled Freshwater Fish.
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Mielcarek K, Puścion-Jakubik A, Gromkowska-Kępka KJ, Soroczyńska J, Karpińska E, Markiewicz-Żukowska R, Naliwajko SK, Moskwa J, Nowakowski P, Borawska MH, and Socha K
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- Animals, Food Preservation, Copper analysis, Fishes metabolism, Fresh Water analysis, Seafood analysis, Selenium analysis, Smoke analysis, Zinc analysis
- Abstract
The aim of the study was to assess the zinc (Zn), copper (Cu) and selenium (Se) content in freshwater fish from Poland. Selected species of raw, smoked and pickled fish were evaluated by atomic absorption spectrometry (AAS). The concentration of Zn, Cu and Se in the examined fish ranged from 1.5 to 49.9 mg/kg, 0.01 to 2.8 mg/kg and 30.9 to 728.2 µg/kg, respectively. One serving of every fish product covered the recommended dietary allowance (RDA) of Zn by 5.38-65.0%, of Cu by 0.42-11.4% and of Se by 12.3-198.6%. A cluster analysis allowed us to distinguish European eel (raw and smoked) based on the Zn content. Additionally, based on the Cu and Se content, pickled common whitefish was differentiated from other species and types of fish products. The discriminant analysis model of smoked fish enabled their classification with a 70% accuracy. Regarding Zn, all forms of the European eel as well as raw and smoked vendace can be considered a source of this element. None of the tested fish can be considered a source of Cu. All products are safe for human consumption with regard to the Zn and Cu content. Almost each form of every species of fish can be considered a source of Se. However, the Se content should be monitored in smoked and pickled common whitefish, pickled bream and pickled vendace.
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- 2020
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10. Biomarkers of neutrophil extracellular traps (NETs) and nitric oxide-(NO)-dependent oxidative stress in women who miscarried.
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Omeljaniuk WJ, Jabłońska E, Garley M, Pryczynicz A, Ratajczak-Wrona W, Socha K, Borawska MH, and Charkiewicz AE
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- Adolescent, Adult, Biomarkers metabolism, Case-Control Studies, Deoxyribonuclease I metabolism, Female, Histones metabolism, Humans, Malondialdehyde metabolism, Peroxidase metabolism, Placenta metabolism, Pregnancy, Protein-Arginine Deiminase Type 4 metabolism, Young Adult, Abortion, Spontaneous metabolism, Abortion, Spontaneous pathology, Extracellular Traps metabolism, Neutrophils cytology, Nitric Oxide metabolism, Oxidative Stress
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Pregnancy loss is a multidisciplinary problem which concerns researchers from the fields of medicine, epidemiology, psychology, and public health. The primary objective of the present study was to explain the potential role of neutrophil extracellular traps (NETs) in the process of spontaneous miscarriage. Enzyme-linked immunosorbent assay to assess the levels of biomarkers of NETs in the serum of examined women was conducted. Furthermore, levels of nitric oxide (NO) and late markers of its action were measured in serum samples. Analyses results demonstrated the existence of NETs in the placental tissue of women who miscarried as well as a simultaneous increase in the levels of myeloperoxidase and pentraxin 3. This clearly confirms the participation of NETs in the course of pregnancy loss. Women who have had a miscarriage but did not show the presence of NETs in their placenta exhibited the highest contents of NO, nitrotyrosine, and malondialdehyde suggesting a different pathway leading to pregnancy loss associated with disturbed oxidative-antioxidative processes. Although study results demonstrate new aspects associated with the formation of NETs they are not, however, sufficient to unambiguously determine the role of NETs in the course of miscarriage.
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- 2020
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11. Modern Methods for Assessing the Quality of Bee Honey and Botanical Origin Identification.
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Puścion-Jakubik A, Borawska MH, and Socha K
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This paper is a summary of the latest literature on methods for assessing quality of natural bee honey. The publication briefly characterizes methods recommended by the International Honey Commission, published in 2009, as well as newer methods published in the last 10 years. Modern methods of assessing honey quality focus mainly on analyzing markers of individual varieties and classifying them into varieties, using, among others, near infrared spectroscopy techniques (NIR), potentiometric tongue, electronic nose, nuclear magnetic resonance (NMR), zymography, polymerase chain reaction (PCR), DNA metabarcoding, and chemometric techniques including partial least squares (PLS), principal component analysis (PCA) and artificial neural networks (ANN). At the same time, effective techniques for analyzing adulteration, sugar, and water content, hydroxymethylfurfural (HMF), polyphenol content, and diastase activity are being sought. Modern techniques enable the results of honey quality testing to be obtained in a shorter time, using the principles of green chemistry, allowing, at the same time, for high precision and accuracy of determinations. These methods are constantly modified, so that the honey that is on sale is a product of high quality. Prospects for devising methods of honey quality assessment include the development of a fast and accurate alternative to the melissopalynological method as well as quick tests to detect adulteration.
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- 2020
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12. Copper, Manganese, Selenium and Zinc in Wild-Growing Edible Mushrooms from the Eastern Territory of "Green Lungs of Poland": Nutritional and Toxicological Implications.
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Mirończuk-Chodakowska I, Socha K, Zujko ME, Terlikowska KM, Borawska MH, and Witkowska AM
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- Poland, Agaricales chemistry, Copper analysis, Manganese analysis, Selenium analysis, Zinc analysis
- Abstract
The aims of this study were to determine Cu, Mn, Se, and Zn content in wild mushrooms collected from unpolluted areas of the eastern Green Lungs of Poland (GLP) territory, to compare them to some popular species of cultivated mushrooms, evaluate mushroom contribution to the daily intake of the studied bioelements, and to determine their possible toxic effect resulting from potentially excessive mushroom consumption from areas recognized as ecologically uncontaminated. Bioelements were determined in 21 species of edible mushrooms: eighteen species of wild mushrooms and three species of popular cultivated mushrooms. The mean Cu, Mn, Se, and Zn content (in µg/g, dry mass DM) ranged from 10.6-123.1, 12.2-41, 0.13-13.3, and 68.3-184, respectively. A comparison with recommended dietary allowance (RDA) for Cu, Se, and Zn as well as adequate intake (AI) for Mn demonstrated that a 100 g fresh mass (FM) portion of mushroom species with the highest content of a given element can meet the demand for Cu, Mn, Se and Zn at 203%, 14-17%, 211%, and 16-22%, respectively. A comparison of the content of the examined bioelements contained in one portion of mushrooms (100 g FM) against the toxicological intake limits for different chemical elements with the provisional maximum tolerable daily intake (PMTDI) and upper intake level (UL) showed no risk of toxicity for the evaluated mushroom species.
- Published
- 2019
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13. Concentration of Zinc, Copper, Selenium, Manganese, and Cu/Zn Ratio in Hair of Children and Adolescents with Myopia.
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Fedor M, Urban B, Socha K, Soroczyńska J, Krętowska M, Borawska MH, and Bakunowicz-Łazarczyk A
- Abstract
The aim of this work was to determine the concentration of trace elements, zinc, copper, selenium, manganese, and Cu/Zn ratio, in scalp hair samples of children and adolescents with myopia. The study included 92 children (mean age 14.5 ± 2.5 years) with myopia and 43 healthy persons (mean age 11.8 ± 4.7 years). Each patient had a complete eye examination. Trace element concentrations in hair were determined by atomic absorption spectrometry. Cu/Zn ratio was also calculated. The zinc level in the hair of myopic patients was significantly higher (260 µ g/g) in comparison to the control group (130 µ g/g). There was a significantly lower Cu/Zn ratio in myopic patients (0.045) compared with controls (0.07). An insignificant difference was observed in the hair level of copper, selenium, and manganese between patients and controls. The results show that trace elements may play a significant role in the pathogenesis of myopia. Further studies should pay more attention to determine the effect of trace element on children myopia.
- Published
- 2019
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14. Selenium, zinc, copper, Cu/Zn ratio and total antioxidant status in the serum of vitiligo patients treated by narrow-band ultraviolet-B phototherapy.
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Wacewicz M, Socha K, Soroczyńska J, Niczyporuk M, Aleksiejczuk P, Ostrowska J, and Borawska MH
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- Adult, Aged, Case-Control Studies, Female, Humans, Male, Middle Aged, Spectrophotometry, Spectrophotometry, Atomic, Vitiligo blood, Young Adult, Antioxidants analysis, Copper blood, Selenium blood, Ultraviolet Therapy, Vitiligo radiotherapy, Zinc blood
- Abstract
Background: Vitiligo is a chronic, depigmenting skin disorder, whose pathogenesis is still unknown. Narrow band ultraviolet-B (NB-UVB) is now one of the most widely used treatment of vitiligo. It was suggested that trace elements may play a role in pathogenesis of vitiligo., Aim: The aim of this study was to estimate the concentration of selenium (Se), zinc (Zn), copper (Cu) and Cu/Zn ratio as well as total antioxidant status (TAS) in the serum of patients with vitiligo., Methods: We assessed 50 patients with vitiligo and 58 healthy controls. Serum levels of Se, Zn and Cu were determined by the atomic absorption spectrometry method, and the Cu/Zn ratio was also calculated. TAS in serum was measured spectrophotometrically., Result: Serum concentration of Se in patients with vitiligo before and after phototherapy was significantly lower as compared to the control group. Zn level in the serum of patients decreased significantly after phototherapy. We observed higher Cu/Zn ratio (p < .05) in examined patients than in the control group and after NB-UVB. We have found decrease in TAS in the serum of vitiligo patients after NB-UVB., Conclusions: The current study showed some disturbances in the serum levels of trace elements and total antioxidant status in vitiligo patients.
- Published
- 2018
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15. Cadmium and Lead in Women Who Miscarried.
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Omeljaniuk WJ, Socha K, Soroczynska J, Charkiewicz AE, Laudanski T, Kulikowski M, Kobylec E, and Borawska MH
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- Abortion, Spontaneous diagnosis, Adolescent, Adult, Cadmium analysis, Female, Humans, Lead analysis, Metals, Heavy analysis, Metals, Heavy blood, Pregnancy, Spectrophotometry, Atomic, Young Adult, Abortion, Spontaneous blood, Cadmium blood, Lead blood, Placenta chemistry
- Abstract
Background: Cadmium (Cd) and lead (Pb) are toxic elements which, when ingested excessively in food and drinking water, accumulate in selected organs and pass through the placenta barrier to the foetus, showing teratogenic effects. The aim of the study was to determine the concentration of Cd and Pb in blood and placental tissue in women who miscarried., Methods: The study group consisted of 83 women who miscarried. The control group included 35 women in the first trimester of pregnancy and after childbirth. The experimental materials consisted of whole blood and fragments of placental tissue. The concentrations of Cd and Pb were determined using atomic absorption spectrometry (AAS) with electrothermal atomization in a graphite furnace and by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) in standard mode., Results: The average concentration of Cd (2.730 ± 2.07 µg/L) and Pb (35.54 ± 11.0 µg/L) in the blood of women with miscarriage was higher in comparison to the level of these toxic metals in the blood of women from the control group (Cd 1.035 ± 0.59 µg/L; Pb 27.11 ± 4.6 µg/L). The average Cd (214.4 ± 514 µg/L) and Pb (199.6 ± 348 µg/L) content in the placenta of women with miscarriage was higher in comparison to the amount of these elements in the placenta of women from the control group (Cd 127.4 ± 85 ng/L; Pb 26.35 ± 7.9 ng/L). Tobacco smoking significantly affected cadmium blood levels and the placental tissue content in women who miscarried., Conclusions: Elevated Cd and Pb concentrations in the blood and placenta of pregnant women may be connected with the occurrence of miscarriage; therefore, the levels of these heavy metals should be monitored in women who plan pregnancy. It seems that determining the level of molar ratio between toxic metal and antioxidant elements can be analyzed as a marker for selection for control examinations as a valuable complement to existing diagnostic procedures in prevention, especially in early pregnancy. Additional diagnostic methods should be established as new tools in perinatal care in order to enable early diagnosis of pregnancy pathology and, especially, to prevent miscarriage.
- Published
- 2018
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16. Concentration of selenium, zinc, copper, Cu/Zn ratio, total antioxidant status and c-reactive protein in the serum of patients with psoriasis treated by narrow-band ultraviolet B phototherapy: A case-control study.
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Wacewicz M, Socha K, Soroczyńska J, Niczyporuk M, Aleksiejczuk P, Ostrowska J, and Borawska MH
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- Adult, Aged, Case-Control Studies, Female, Humans, Male, Middle Aged, Young Adult, Antioxidants metabolism, C-Reactive Protein metabolism, Copper blood, Psoriasis blood, Psoriasis radiotherapy, Selenium blood, Ultraviolet Therapy, Zinc blood
- Abstract
Background: Psoriasis is a common, an inflammatory skin disease. Trace elements may play an active role in the pathogenesis of psoriasis., Objective: The aim of this study was to estimate the concentration of selenium (Se), zinc (Zn), copper (Cu) and Cu/Zn ratio as well as total antioxidant status (TAS) and c-reactive protein (CRP) in the serum of patients with psoriasis., Methods: In this case-control study sixty patients with psoriasis and fifty-eight healthy people were examined. Serum levels of Se, Zn and Cu were determined by atomic absorption spectrometry. Cu/Zn ratio was calculated. TAS was measured spectrophotometrically. CRP was analyzed by immunoturbidimetric method. Clinical activity of psoriasis was evaluated using Psoriasis Area and Severity Index (PASI)., Results: Serum concentration of Se in patients with psoriasis (71.89±16.90μg/L) was lower as compared to the control group (79.42±18.97μg/L) and after NB-UVB. Cu level of patients was higher (1.151±0.320mg/L) as compared to controls (1.038±0.336mg/L), but Zn level did not differ. We observed higher Cu/Zn ratio (p<0.05) in examined patients than in the control group and after NB-UVB. We found decrease TAS before and after NB-UVB. CRP levels was found to be normal range. A significant correlation coefficient between CRP and Cu/Zn was observed., Conclusions: The study showed some disturbances in the serum levels of trace elements and TAS in psoriatic patients., (Copyright © 2017 The Authors. Published by Elsevier GmbH.. All rights reserved.)
- Published
- 2017
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17. Dietary habits; concentration of copper, zinc, and Cu-to-Zn ratio in serum and ability status of patients with relapsing-remitting multiple sclerosis.
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Socha K, Karpińska E, Kochanowicz J, Soroczyńska J, Jakoniuk M, Wilkiel M, Mariak ZD, and Borawska MH
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- Adolescent, Adult, Aged, Case-Control Studies, Diet statistics & numerical data, Female, Humans, Male, Middle Aged, Spectrophotometry, Atomic, Young Adult, Copper blood, Diet methods, Feeding Behavior, Multiple Sclerosis blood, Zinc blood
- Abstract
Objective: Dietary habits and adequate intake of antioxidants in the diet-for example, copper (Cu) and zinc (Zn)-may be an environmental factor in the occurrence of multiple sclerosis (MS). The aim of this study was to estimate the influence of dietary habits on the concentration of Cu, Zn in the serum, and the effect of Cu-to-Zn ratio on the ability status of patients with relapsing-remitting MS., Methods: This was an observational case-control study that included 101 individuals with MS and 68 healthy individuals (controls). Food frequency questionnaires were used to collect dietary data. Serum concentrations of Cu and Zn were determined by the electrothermal and flame atomic absorption spectrometry method, respectively. The ratio of Cu to Zn was calculated and compared with the Expanded Disability Status Scale of patients., Results: The concentration of Zn was significantly lower in the serum of individuals with MS (0.776 ± 0.195 mg/L) than in the control group (0.992 ± 0.315 mg/L). The ratio of Cu to Zn was higher in the examined patients (1.347 ± 0.806) than in the healthy volunteers (1.012 ± 0.458). Lower ability status (P < 0.05) was revealed in patients with an abnormal ratio of Cu to Zn, particularly, in cerebellar function, pyramidal tracts, and emotional conditions. Selected dietary habits have a significant influence on Cu and Zn concentration in the serum of patients with MS., Conclusions: Lower serum concentrations of Zn and higher ratio of Cu to Zn in patients with MS can suggest a relationship between MS and oxidative stress. Products that are a source of Zn should be included in the diet, which can improve the clinical condition of people with MS., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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18. Cadmium, arsenic, selenium and iron- Implications for tumor progression in breast cancer.
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Jablonska E, Socha K, Reszka E, Wieczorek E, Skokowski J, Kalinowski L, Fendler W, Seroczynska B, Wozniak M, Borawska MH, and Wasowicz W
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- Adult, Aged, Aged, 80 and over, Breast chemistry, Breast Neoplasms pathology, Female, Humans, Middle Aged, Smoking, Arsenic analysis, Breast Neoplasms chemistry, Cadmium analysis, Iron analysis, Selenium analysis
- Abstract
The aim of this study was to determine Cd (cadmium) and As (arsenic) contents in human breast cancer tissues, investigate their interactions with Se (selenium) and Fe (iron), and assess their further implications for tumor progression. Metal contents were determined in 42 tissue sets (tumor and adjacent tissue) collected from 42 women diagnosed with primary breast cancer. Analytical methods included AAS and ICP-MS techniques. Significantly higher contents of Cd (p=0.0003), Se (p<0.0001) and Fe (p=0.0441) whereas significantly lower content of As (p<0.0001) were observed in tumors as compared to adjacent tissues. There was a significant positive correlation between Cd and As contents in tumor tissue. However, only Cd was significantly associated with histological type of tumor, its size, grading and progesterone receptor status. This study support the role of Cd in breast cancer risk and progression. The possible link between As exposure and breast cancer is still not clear., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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19. Serum Concentration of Zinc, Copper, Selenium, Manganese, and Cu/Zn Ratio in Children and Adolescents with Myopia.
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Fedor M, Socha K, Urban B, Soroczyńska J, Matyskiela M, Borawska MH, and Bakunowicz-Łazarczyk A
- Subjects
- Adolescent, Antioxidants analysis, Child, Female, Humans, Male, Myopia diagnosis, Myopia prevention & control, Oxidative Stress, Spectrophotometry, Atomic, Trace Elements blood, Copper blood, Manganese blood, Myopia blood, Selenium blood, Zinc blood
- Abstract
The purpose of the present study was the assessment of the serum concentration of antioxidant microelements-zinc, copper, selenium, manganese, and Cu/Zn ratio in children and adolescents with myopia. Eighty-three children were examined (mean age 14.36 ± 2.49 years) with myopia. The control group was 38 persons (mean age 12.89 ± 3.84 years). Each patient had complete eye examination. The serum concentration of zinc, copper, manganese, and selenium was determined by atomic absorption spectrometry. Cu/Zn ratio, which is the indicator of the oxidative stress, was also calculated. The average serum concentration of zinc in myopic patients was significantly lower (0.865 ± 0.221 mg L
-1 ) in comparison to the control group (1.054 ± 0.174 mg L-1 ). There was significantly higher Cu/Zn ratio in myopic patients (1.196 ± 0.452) in comparison to that in the control group (0.992 ± 0.203). The average serum concentration of selenium in the study group was significantly lower (40.23 ± 12.07 μg L-1 ) compared with that in the control group (46.00 ± 12.25 μg L-1 ). There were no essential differences between serum concentration of copper and manganese in the study group and the control group. Low serum concentration of zinc and selenium in myopic children may imply an association between insufficiency of these antioxidant microelements and the development of the myopia and could be the indication for zinc and selenium supplementation in the prevention of myopia. Significantly, higher Cu/Zn ratio in the study group can suggest the relationship between myopia and oxidative stress.- Published
- 2017
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20. The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism.
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Jablonska E, Reszka E, Gromadzinska J, Wieczorek E, Krol MB, Raimondi S, Socha K, Borawska MH, and Wasowicz W
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- Adult, Antigens, CD blood, Antigens, CD metabolism, Blood Glucose metabolism, Dietary Supplements, Down-Regulation drug effects, Fasting blood, Female, Genes, myc drug effects, Glycated Hemoglobin analysis, Glycated Hemoglobin drug effects, Homeostasis, Humans, Lactate Dehydrogenases blood, Lactate Dehydrogenases metabolism, Male, Mixed Function Oxygenases blood, Mixed Function Oxygenases metabolism, Pyruvate Dehydrogenase (Lipoamide) blood, Pyruvate Dehydrogenase (Lipoamide) metabolism, RNA, Messenger blood, RNA, Messenger isolation & purification, Receptor, Insulin blood, Receptor, Insulin metabolism, Receptors, Adiponectin blood, Receptors, Adiponectin metabolism, Repressor Proteins blood, Repressor Proteins metabolism, Selenium administration & dosage, Trace Elements administration & dosage, Blood Glucose drug effects, Blood Glucose genetics, Gene Expression Regulation drug effects, Selenium pharmacology, Trace Elements pharmacology
- Abstract
The aim of the study was to evaluate the effect of selenium supplementation on the expression of genes associated with glucose metabolism in humans, in order to explain the unclear relationship between selenium and the risk of diabetes. For gene expression analysis we used archival samples of cDNA from 76 non-diabetic subjects supplemented with selenium in the previous study. The supplementation period was six weeks and the daily dose of selenium was 200 µg (as selenium yeast). Blood for mRNA isolation was collected at four time points: before supplementation, after two and four weeks of supplementation, and after four weeks of washout. The analysis included 15 genes encoding selected proteins involved in insulin signaling and glucose metabolism. In addition, HbA1c and fasting plasma glucose were measured at three and four time points, respectively. Selenium supplementation was associated with a significantly decreased level of HbA1c but not fasting plasma glucose (FPG) and significant down-regulation of seven genes: INSR , ADIPOR1 , LDHA , PDHA , PDHB , MYC , and HIF1AN . These results suggest that selenium may affect glycemic control at different levels of regulation, linked to insulin signaling, glycolysis, and pyruvate metabolism. Further research is needed to investigate mechanisms of such transcriptional regulation and its potential implication in direct metabolic effects., Competing Interests: The authors declare no conflict of interest.
- Published
- 2016
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21. DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: a short-term supplementation trial with respect to GPX1 and SEPP1 polymorphism.
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Jablonska E, Raimondi S, Gromadzinska J, Reszka E, Wieczorek E, Krol MB, Smok-Pieniazek A, Nocun M, Stepnik M, Socha K, Borawska MH, and Wasowicz W
- Subjects
- Adolescent, Adult, Alleles, Body Mass Index, Female, Genotype, Genotyping Techniques, Glutathione Peroxidase blood, Humans, Lipid Peroxidation drug effects, Male, Middle Aged, Polymorphism, Single Nucleotide, RNA, Messenger genetics, RNA, Messenger metabolism, Saccharomyces cerevisiae, Selenium administration & dosage, Selenium blood, Selenoproteins blood, Young Adult, Glutathione Peroxidase GPX1, DNA Damage drug effects, Dietary Supplements, Glutathione Peroxidase genetics, Oxidative Stress drug effects, Selenium toxicity, Selenoproteins genetics
- Abstract
Purpose: Selenium, both essential and toxic element, is considered to protect against cancer, though human supplementation trials have generated many inconsistent data. Genetic background may partially explain a great variability of the studies related to selenium and human health. The aim of this study was to assess whether functional polymorphisms within two selenoprotein-encoding genes modify the response to selenium at the level of oxidative stress, DNA damage, and mRNA expression, especially in the individuals with a relatively low selenium status., Methods: The trial involved 95 non-smoking individuals, stratified according to GPX1 rs1050450 and SEPP1 rs3877899 genotypes, and supplemented with selenium yeast (200 µg) for 6 weeks. Blood was collected at four time points, including 4 weeks of washout., Results: After genotype stratification, the effect of GPX1 rs1050450 on lower GPx1 activity responsiveness was confirmed; however, in terms of DNA damage, we failed to indicate that individuals homozygous for variant allele may especially benefit from the increased selenium intake. Surprisingly, considering gene and time interaction, GPX1 polymorphism was observed to modify the level of DNA strand breaks during washout, showing a significant increase in GPX1 wild-type homozygotes. Regardless of the genotype, selenium supplementation was associated with a selectively suppressed selenoprotein mRNA expression and inconsistent changes in oxidative stress response, indicating for overlapped, antioxidant, and prooxidant effects. Intriguingly, DNA damage was not influenced by supplementation, but it was significantly increased during washout., Conclusions: These results point to an unclear relationship between selenium, genotype, and DNA damage., Competing Interests: Compliance with ethics guidelines All the procedures performed in the studies involving human participants were in accordance with the ethical standards of the Institutional and/or National Research Committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Conflict of interest On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Published
- 2016
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22. Anti-proliferative and anti-migration effects of Polish propolis combined with Hypericum perforatum L. on glioblastoma multiforme cell line U87MG.
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Borawska MH, Naliwajko SK, Moskwa J, Markiewicz-Żukowska R, Puścion-Jakubik A, and Soroczyńska J
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- Antineoplastic Agents chemistry, Cell Line, Tumor, Glioblastoma, Humans, Plant Extracts chemistry, Propolis chemistry, Antineoplastic Agents pharmacology, Cell Movement drug effects, Cell Proliferation drug effects, Hypericum chemistry, Plant Extracts pharmacology, Propolis pharmacology
- Abstract
Background: Propolis and Hypericum perforatum L. are natural products which contain many active compounds and have numerous beneficial effects, including an antitumor effect. Gliobmastoma multiforme (GBM) is a common primary brain tumor with poor prognosis and limited treatment options. In this study, the effect of propolis (EEP) combined with H. perforatum L. (HPE) on glioblastoma cell line U87MG was investigated for the first time., Methods: Anti-proliferative activity of EEP, HPE and their combination (EEP + HPE) was determined by a cytotoxicity test, DNA binding by [(3)H]-thymidine incorporation and cell migration assay. Anti-metastatic properties in U87MG treated with EEP, HPE and EEP + HPE were estimated on cells migration test (scratch assay) and metalloproteinases (MMP2 and MMP9) secretion (gelatin zymography)., Results: Combination of HPE and EEP extracts was found to have a time- and dose-dependent inhibitory effect on the viability of U87MG cells. This effect was significantly higher (p < 0.05) when compared to these two extracts applied separately, which was confirmed by the significant reduction of DNA synthesis and significantly higher mitochondrial membrane permeabilization. A significant decreasing in migration cells and in pro-MMP9 and pro-MMP2 secretion in U87MG cells were demonstrated after exposure to combination of EEP (30 μg/ml) with HPE (6.25 μg/ml)., Conclusions: In this study, the combination of ethanolic extract from propolis and ethanolic extract of fresh-cut H. perforatum L. was proved the ability to reduce invasiveness of glioma cells through the inhibition of MMP2 and MMP9 secretion and suppression of cell migration. It has a more potent anti-proliferative effect on U87MG glioma cell line compared to using propolis and H. perforatum L. separately. Further studies are required to verify whether the examined extracts can activate apoptotic pathways.
- Published
- 2016
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23. Selenium, Zinc, Copper, and Total Antioxidant Status in the Serum of Patients with Chronic Tonsillitis.
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Michalska-Mosiej M, Socha K, Soroczyńska J, Karpińska E, Łazarczyk B, and Borawska MH
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- Adult, Chronic Disease, Female, Humans, Male, Middle Aged, Young Adult, Antioxidants metabolism, Copper blood, Selenium blood, Tonsillitis blood, Zinc blood
- Abstract
Antioxidants can play a significant role in chronic inflammatory process. The aim of this study was to evaluate the content of selenium (Se), zinc (Zn), copper (Cu), and total antioxidant status (TAS) of patients with chronic tonsillitis (CT). The study group consisted of 84 patients with CT from 18 to 62 years old and the control group of 67 healthy people aged 19-65 years. Se, Zn, and Cu concentration in serum samples were determined by atomic absorption spectrometry. Serum TAS was measured spectrophotometrically, using the test by Randox Laboratories-Us Ltd. The mean content of Se and Zn in the serum of patients with CT (61.122 ± 12.73 μg/L, 0.887 ± 0.26 mg/L, respectively) was lower compared to the control group (77.969 ± 12.73 μg/L, 0.993 ± 0.32 mg/L, respectively). The mean serum concentration of Cu in patients with CT (1.219 ± 0.35 mg/L) was higher compared to its serum concentration in healthy people (1.033 ± 0.37 mg/L). Serum TAS of patients with CT (1.171 ± 0.33 mmol/L) was lower in comparison with healthy volunteers (1.333 ± 0.42 mmol/L). The serum concentration of Se, Zn, and TAS in patients with CT was lower, whereas the concentration of Cu was higher compared to healthy volunteers. Smoking has an influence on reducing the concentration of Se and TAS of patients with CT.
- Published
- 2016
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24. Serum Levels of Biomarkers of Immune Activation and Associations With Neurological Impairment in Relapsing-Remitting Multiple Sclerosis Patients During Remission.
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Witkowska AM, Socha K, Kochanowicz J, Karpińska E, Jakoniuk M, Zujko ME, Wilkiel M, Borawska MH, and Mariak Z
- Subjects
- Adult, Case-Control Studies, Female, Humans, Male, Middle Aged, Poland, Biomarkers blood, Disease Progression, E-Selectin blood, Intercellular Adhesion Molecule-1 blood, Multiple Sclerosis physiopathology, Tumor Necrosis Factor-alpha blood
- Abstract
Introduction: Although much is known about cytokines and adhesion molecules during an active course of multiple sclerosis (MS), there is limited information about their serum levels during remission., Objective: This study aimed to (1) compare peripheral levels of tumor necrosis factor-α (TNF-α), soluble interleukin-2 receptor α (sIL-2Rα), soluble intercellular adhesion molecule-1 (sICAM-1), and soluble E-selectin (sE-selectin) in MS patients during clinical remission with those of healthy controls and (2) explore possible relationships between the levels of these cytokines and adhesion molecules and neurological impairment., Methods: Initially, 92 patients with relapsing-remitting multiple sclerosis (RRMS) who were in clinical remission and 30 healthy controls were recruited for this study. The severity of neurological impairment was assessed with the Expanded Disability Status Scale (EDSS). Serum concentrations of TNF-α, sIL-2Rα, sICAM-1, and sE-selectin were determined using the sandwich enzyme-linked immunosorbent (ELISA) technique and compared between patients and controls. In a subset of RRMS patients (n = 67), the levels of these cytokines and adhesion molecules were compared between subgroups of patients based on scores on the EDSS subscales, which measure disability level for specific neurological functions., Results: The MS patients' TNF-α, sICAM-1, and sE-selectin levels were markedly lower than those of the controls, while their sIL-2Rα level was higher. The serum sICAM-1 concentration was positively associated with EDSS total score (ρ = .291, p = .017) as well as with the EDSS pyramidal (ρ = .267, p = .029) and cerebellar subscores (ρ = .303, p = .013). In the patients with cerebellar deficits and severe brain stem dysfunction, sICAM-1 levels were upregulated., Conclusion: Although a decreased sICAM-1 concentration was observed in RRMS patients in remission as compared to healthy controls, sICAM-1 seemed to reflect neurological impairment and clinical disability. These data suggest that increasing serum sICAM-1 levels may be associated with progression of cerebellar or brain stem perturbations. However, further studies are required to confirm these findings in a larger population of RRMS patients., (© The Author(s) 2015.)
- Published
- 2016
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25. Bakery products as a source of total dietary fiber in young adults.
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Markiewicz-Żukowska R, Moskwa J, Gromkowska-Kępka K, Laskowska E, Laskowska J, Tomczuk J, and Borawska MH
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- Dietary Fiber analysis, Female, Humans, Lignans analysis, Male, Nutritive Value, Poland, Young Adult, Bread analysis, Dietary Fiber administration & dosage, Energy Intake, Feeding Behavior, Flour analysis, Lignans administration & dosage
- Abstract
Background: Bakery products are a source of bioactive compounds, such as dietary fibre (DF), whose proper supply plays an important role in prevention of civilisation diseases., Objective: The aim of the present study was to determine total dietary fibre (TDF) content in bakery products and their contribution to TDF supply., Material and Methods: The determination of TDF content was performed using enzymatic-gravimetric method in 72 samples of six types of bakery products (wholemeal rye bread, wheat-rye bread, wheat-rye bread with grains, toast bread, crispbread, rolls) included in the young adults diet. Simultaneously, frequency of bakery products consumption and their contribution to TDF supply were assessed based on dietary interview questionnaires carried out among 224 students from Poland. Index of nutritional quality (INQ) of examined bakery products was calculated., Results: Our data indicate that average TDF content depended on the type of bakery products and ranged from 2.19 g/100 g in rolls to 11.80 g/100 g in wholemeal rye bread. All of the tested types of bakery products, except rolls, were a good source of fibre (INQ≥1), but the richest were wholemeal rye and wheat-rye with grains breads. Analysis of questionnaires data showed that bakery products were regularly consumed by 80% of young adults; however, most of whom preferred rolls. Consumption of bakery products covered current recommendations for dietary fibre in 27%. Daily intake of bakery products and TDF was not correlated with student's BMI, however, women frequently consuming bread had a lower BMI than those who rarely ate it., Conclusion: Wholemeal rye and wheat-rye with grains breads are the rich source of TDF and they should be consumed by young adults in order to achieve the recommended TDF values.
- Published
- 2016
26. Antioxidant status in women who have had a miscarriage.
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Omeljaniuk WJ, Socha K, Borawska MH, Charkiewicz AE, Laudański T, Kulikowski M, and Kobylec E
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- Adolescent, Adult, Copper blood, Female, Glutathione Peroxidase blood, Humans, Manganese blood, Selenium blood, Superoxide Dismutase blood, Trace Elements blood, Young Adult, Zinc blood, Abortion, Spontaneous blood, Antioxidants metabolism
- Abstract
Purpose: During normal pregnancy there is an oxido-reductive balance between action of pro-oxidative factors. The aim of this study was to evaluate the total antioxidant status (TAS) and glutathione peroxidase (GSH-Px) activity, and the content of selenium (Se), zinc (Zn), copper (Cu) and manganese (Mn) in women who have had a miscarriage., Patients and Methods: The study group consisted of 83 women who had had miscarriages. The control group included 35 women in the first trimester of pregnancy, and 35 pregnant women after childbirth., Results: TAS activity and Cu concentration in serum in women who experienced a miscarriage were significantly lower, but Mn level - higher, than in women in the first trimester of pregnancy. The content of Se, Cu and Mn in placental tissue in patients who have had a miscarriage was significantly higher, while Zn content was lower than in pregnant women at full-term delivery., Conclusions: Our findings, provided in the research, enable us to claim that the total antioxidative status is significantly lower in women who have had a miscarriage. Low level of Zn but high of Mn in the examined biological material may be indicative to the incidence of miscarriage., (Copyright © 2015 Medical University of Bialystok. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.)
- Published
- 2015
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27. Serum zinc concentrations correlate with mental and physical status of nursing home residents.
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Markiewicz-Żukowska R, Gutowska A, and Borawska MH
- Subjects
- Aged, Aged, 80 and over, Cognition, Depression blood, Female, Humans, Male, Memory Disorders blood, Memory Disorders physiopathology, Middle Aged, Sex Characteristics, Statistics, Nonparametric, Mental Health, Nursing Homes, Zinc blood
- Abstract
Introduction: Zinc (Zn) is one of the most important trace elements in the body. Zn deficiency seems to play a role in the development of age-related diseases and impairment of quality of life. Zn status has been especially studied in free-living or hospitalised people, but data from older residents of nursing homes are scarce. This study aimed to determine the Zn status among the older individuals in correlation to their mental and physical performance., Methods: A total of 100 participants aged between 60-102 years were recruited between October 2010 and May 2012 at the nursing home in Bialystok (Poland). Zn status was evaluated by determining the concentration in serum by flame atomic absorption spectrometry. Anthropometric variables and fitness score (FS) were measured. Abbreviated Mental Test Score (AMTS), Geriatric Depression Scale (GDS), Self-Rated Health (SRH), independence in Activities of Daily Living (ADL) were recorded., Results and Discussion: The mean serum Zn concentration was 0.83 ± 0.20 mg/L, 28% of residents had Zn deficiency. Cognitive functions were impaired (AMTS ≤ 8) in 45% of the studied persons and 48% showed depressive symptoms (GDS ≥ 1). The ability to independently perform activities of daily living (ADL = 6) was found in 61% of participants, but most of them (90%) had weak body type (FS < 70), correlating with GDS, SRH and body mass index (BMI). Serum Zn concentration correlated with mental efficiency and was statistically significantly higher in older people with normal cognitive function and without depression than in patients with memory impairment and showing depressive symptoms., Conclusions: Nursing home residents seem at risk of marginal Zn status, which correlates with their mental status as measured by the AMTS and GDS. Their low FS is associated with mental health deterioration and obesity.
- Published
- 2015
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28. Dietary habits and selenium, glutathione peroxidase and total antioxidant status in the serum of patients with relapsing-remitting multiple sclerosis.
- Author
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Socha K, Kochanowicz J, Karpińska E, Soroczyńska J, Jakoniuk M, Mariak Z, and Borawska MH
- Subjects
- Adolescent, Adult, Female, Humans, Male, Middle Aged, Antioxidants metabolism, Feeding Behavior, Glutathione Peroxidase blood, Multiple Sclerosis, Relapsing-Remitting physiopathology, Selenium blood
- Abstract
Background: Dietary habits and adequate dietary intake of antioxidants in the diet may be one of the most important environmental factors for the prevention of Multiple Sclerosis (MS)., Objectives: The aim of this study was to estimate selenium (Se) concentration, glutathione peroxidase (GSH-Px) activity and total antioxidant status (TAS) in the serum of patients with MS and the influence of dietary habits on the status., Methods: 101 patients with relapsing-remitting MS (aged 18-58 years), as well as control group of 63 healthy people (aged 19-65 years) were studied. Food-frequency questionnaires were implemented to collect the dietary data. Se concentration in the serum samples was determined by atomic absorption spectrometry. GSH-Px activity and TAS in examined serum was measured using the ready-made sets of tests by Randox Laboratories Ltd., UK., Results: Serum Se concentration and GSH-Px activity in the serum of patients with MS (55.2±16.2 μg/L, 6676.1±2386.4 U/L; respectively) were significantly decreased (p<0.01, p<0.05; respectively) compared with control group (79.2±20.6 μg/L, 8029.9±2650.1 U/L; respectively). A significant correlation (r=0.39, p<0.01) was observed between Se concentration and GSH-Px activity in the serum of examined patients. TAS value in the serum of patients with MS (1.03±0.37 mmol/L) was also significantly lower (p<0.01) than in healthy volunteers (1.48±0.41 mmol/L). Frequent consumption of poultry, bakery products, pulses and fish seemed to increase serum Se concentration in the group of patients; whereas frequent consumption of butter, wholegrain bread, sweet beverages and sugar was found to accompany with lower values of Se in the serum. We have observed significant decrease TAS (p<0.05, p<0.01; respectively) in the serum of smokers and those patients who received immunomodulatory drugs (0.95±0.39 mmol/L, 0.92±0.34 mmol/L; respectively) compared with no-smoking patients and not taking immunomodulators (1.14±0.33 mmol/L, 1.31±0.31 mmol/L; respectively)., Conclusions: Serum Se concentration, GSH-Px activity and TAS value were significantly lower in patients with relapsing-remitting MS compared with healthy volunteers. Dietary habits have a significant influence on Se status. Smoking cigarettes and intake of immunomodulatory drugs therapy have a negative impact on TAS of examined patients.
- Published
- 2014
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29. Polish natural bee honeys are anti-proliferative and anti-metastatic agents in human glioblastoma multiforme U87MG cell line.
- Author
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Moskwa J, Borawska MH, Markiewicz-Zukowska R, Puscion-Jakubik A, Naliwajko SK, Socha K, and Soroczynska J
- Subjects
- Animals, Antineoplastic Agents analysis, Bees, Biological Products analysis, Cell Line, Tumor, Dacarbazine analogs & derivatives, Dacarbazine pharmacology, Humans, Neoplasm Metastasis prevention & control, Temozolomide, Antineoplastic Agents pharmacology, Biological Products pharmacology, Cell Proliferation drug effects, Glioblastoma drug therapy, Honey analysis
- Abstract
Honey has been used as food and a traditional medicament since ancient times. However, recently many scientists have been concentrating on the anti-oxidant, anti-proliferative, anti-inflammatory and other properties of honey. In this study, we investigated for the first time an anticancer effect of different honeys from Poland on tumor cell line - glioblastoma multiforme U87MG. Anti-proliferative activity of honeys and its interferences with temozolomide were determined by a cytotoxicity test and DNA binding by [H3]-thymidine incorporation. A gelatin zymography was used to conduct an evaluation of metalloproteinases (MMP-2 and MMP-9) expression in U87MG treatment with honey samples. The honeys were previously tested qualitatively (diastase activity, total phenolic content, lead and cadmium content). The data demonstrated that the examined honeys have a potent anti-proliferative effect on U87MG cell line in a time- and dose-dependent manner, being effective at concentrations as low as 0.5% (multifloral light honey - viability 53% after 72 h of incubation). We observed that after 48 h, combining honey with temozolomide showed a significantly higher inhibitory effect than the samples of honey alone. We observed a strong inhibition of MMP-2 and MMP-9 for the tested honeys (from 20 to 56% and from 5 to 58% compared to control, respectively). Our results suggest that Polish honeys have an anti-proliferative and anti-metastatic effect on U87MG cell line. Therefore, natural bee honey can be considered as a promising adjuvant treatment for brain tumors.
- Published
- 2014
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30. The interaction of bee products with temozolomide in human diffuse astrocytoma, glioblastoma multiforme and astroglia cell lines.
- Author
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Borawska MH, Markiewicz-Żukowska R, Naliwajko SK, Moskwa J, Bartosiuk E, Socha K, Surażyński A, Kochanowicz J, and Mariak Z
- Subjects
- Adult, Animals, Astrocytes metabolism, Bees, Cell Line, Tumor, Dacarbazine pharmacology, Humans, Immunohistochemistry, Male, Matrix Metalloproteinase 2 genetics, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, NF-kappa B genetics, NF-kappa B metabolism, Specimen Handling, Temozolomide, Astrocytes drug effects, Astrocytoma pathology, Dacarbazine analogs & derivatives, Fatty Acids pharmacology, Glioblastoma pathology, Honey analysis, Propolis pharmacology
- Abstract
In the present study, we investigated the influence of extracts from Salix spp. honey (ESH), beebread (EBB), and royal jelly (ERJ) with and without temozolomide (TMZ) on cell lines derived from a patient with diffuse astrocytoma (DASC), human glioblastoma multiforme (U87MG), and normal human astroglia (SVGp12). DASC was identified by immunocytochemistry. TMZ (20 μM) in combination with ESH (30 μg/mL), EBB (50 μg/mL), and ERJ (30 μg/mL) has stronger cytotoxic activity on U87MG cells after 72 h (20.0, 26.5, and 29.3% of control, respectively) than TMZ alone (about 6% of control). An increase of the cytotoxic effect and inhibition of DNA synthesis in SVGp12 were detected after administering TMZ with the studied extracts. NF-κB p50 subunit was reduced in U87MG cells after treatment with ESH (70.9%) and ESH + TMZ (74.7%). A significant decline of MMP-9 and MMP-2 secretion in cultured U87MG was detected after incubation with EBB (42.9% and 73.0%, respectively) and EBB + TMZ (38.4% and 68.5%, respectively). In conclusion, the use of bee products may increase the cytotoxic effect of TMZ in U87MG but also in SVGp12 cell line. It is important to note that the U87MG cells were sensitive to natural bee products, although there was no influence of natural bee products on the DASC cells.
- Published
- 2014
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31. Radioactivity of honeys from Poland after the Fukushima accident.
- Author
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Borawska MH, Kapała J, Puścion-Jakubik A, Horembała J, and Markiewicz-Żukowska R
- Subjects
- Cesium Radioisotopes analysis, Poland, Potassium Radioisotopes analysis, Radioactivity, Risk Assessment, Fukushima Nuclear Accident, Honey analysis, Radiation Monitoring, Radioactive Pollutants analysis
- Abstract
Concentration of radioactive isotopes in honey constitutes an important bioindicator of environmental radiation. One hundred six honey samples were collected from hives and from bottled honey provided by beekeepers from north-eastern Poland in 2010, before the Fukushima accident, and during the two-year period directly following this catastrophe (2011-2012). Cesium-137 (Cs-137) and potassium-40 (K-40) were determined in lime, multifloral, buckwheat, honeydew and other kinds of honey samples. The obtained mean concentrations of Cs-137 and K-40 (Bq kg(-1)) in honey samples were: 1.19 and 32.92 in 2010, 0.90 and 31.13 in 2011, 1.31 and 36.06 in 2012, respectively. Significant differences were not observed. Therefore, the studied honey samples collected after the Fukushima accident are found to be safe for humans with levels of Cs-137 and K-40 not posing any threats. However, the total concentration of Cs-137 and K-40 in samples stopped decreasing in 2010-2011 and showed a slight increase in 2012. This relation may suggest the impact of pollution from Fukushima and requires further research in the coming years.
- Published
- 2013
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32. Berberis vulgaris root extract alleviates the adverse effects of heat stress via modulating hepatic nuclear transcription factors in quails.
- Author
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Sahin K, Orhan C, Tuzcu M, Borawska MH, Jabłonski J, Guler O, Sahin N, and Hayirli A
- Subjects
- Animals, Biomarkers metabolism, Catalase metabolism, Female, Gene Expression Profiling, Gene Expression Regulation, Enzymologic, Glutathione Peroxidase metabolism, Heme Oxygenase-1 metabolism, Hot Temperature, Lipid Peroxidation, Liver drug effects, Malondialdehyde metabolism, Plant Roots chemistry, Superoxide Dismutase metabolism, Berberis chemistry, Coturnix physiology, Liver metabolism, Oxidative Stress drug effects, Plant Extracts pharmacology
- Abstract
To evaluate the action mode of Berberis vulgaris root extract in the alleviation of oxidative stress, female Japanese quails (n 180, aged 5 weeks) were reared, either at 22°C for 24 h/d (thermoneutral, TN) or 34°C for 8 h/d (heat stress, HS), and fed one of three diets: diets containing 0, 100 or 200 mg of B. vulgaris root extract per kg for 12 weeks. Exposure to HS depressed feed intake by 8·5% and egg production by 12·1%, increased hepatic malondialdehyde (MDA) level by 98·0% and decreased hepatic superoxide dismutase, catalase and glutathione peroxidase activities by 23·5, 35·4 and 55·7%, respectively (P<0·001 for all). There were also aggravations in expressions of hepatic NF-κB and heat-shock protein 70 (HSP70) by 42 and 43%, respectively and suppressions in expressions of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and haeme-oxygenase 1 (HO-1) by 57 and 61%, respectively, in heat-stressed quails (P<0·001 for all). As supplemental B. vulgaris extract increased, there were linear increases in performance parameters, activities of antioxidant enzymes and hepatic Nrf2 and HO-1 expressions (P<0·001 for all) and linear decreases in hepatic MDA level and NF-κB and HSP70 expressions at a greater extent in quails reared under TN condition and those reared under HS condition. In conclusion, dietary supplementation of B. vulgaris root extract to quails reduces the detrimental effects of oxidative stress and lipid peroxidation resulting from HS via activating the host defence system at the cellular level.
- Published
- 2013
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33. Propolis changes the anticancer activity of temozolomide in U87MG human glioblastoma cell line.
- Author
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Markiewicz-Żukowska R, Borawska MH, Fiedorowicz A, Naliwajko SK, Sawicka D, and Car H
- Subjects
- Antineoplastic Agents pharmacology, Antineoplastic Agents, Alkylating pharmacology, Antineoplastic Agents, Alkylating therapeutic use, Biological Transport, Cell Line, Tumor, Cell Nucleus, Cell Proliferation drug effects, Cell Survival drug effects, Dacarbazine pharmacology, Dacarbazine therapeutic use, Dose-Response Relationship, Drug, Drug Synergism, Glioblastoma metabolism, Humans, Propolis pharmacology, Temozolomide, Antineoplastic Agents therapeutic use, Apitherapy, Dacarbazine analogs & derivatives, Glioblastoma drug therapy, NF-kappa B metabolism, Propolis therapeutic use
- Abstract
Background: Propolis is a honey bee product which contains many active compounds, such as CAPE or chrysin, and has many beneficial activities. Recently, its anti-tumor properties have been discussed. We have tested whether the ethanolic extract of propolis (EEP) interferes with temozolomide (TMZ) to inhibit U87MG cell line growth., Methods: The U87MG glioblastoma cell line was exposed to TMZ (10-100 μM), EEP (10-100 μg/ml) or a mixture of TMZ and EEP during 24, 48 or 72 hours. The cell division was examined by the H3-thymidine incorporation, while the western blot method was used for detection of p65 subunit of NF-κB and ELISA test to measure the concentration of its p50 subunit in the nucleus., Results: We have found that both, TMZ and EEP administrated alone, had a dose- and time-dependent inhibitory effect on the U87MG cell line growth, which was manifested by gradual reduction of cell viability and alterations in proliferation rate. The anti-tumor effect of TMZ (20 μM) was enhanced by EEP, which was especially well observed after a short time of exposition, where simultaneous usage of TMZ and EEP resulted in a higher degree of growth inhibition than each biological factor used separately. In addition, cells treated with TMZ presented no changes in NF-κB activity in prolonged time of treatment and EEP only slightly reduced the nuclear translocation of this transcription factor. In turn, the combined incubation with TMZ and EEP led to an approximately double reduction of NF-κB nuclear localization., Conclusions: We conclude that EEP presents cytotoxic properties and may cooperate with TMZ synergistically enhancing its growth inhibiting activity against glioblastoma U87MG cell line. This phenomenon may be at least partially mediated by a reduced activity of NF-κB.
- Published
- 2013
- Full Text
- View/download PDF
34. The effect of Cirsium arvense extract on antioxidant status in quail.
- Author
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Orhan C, Sahin N, Akdemir F, Markiewicz-Zukowska R, Borawska MH, Isidorov VA, Hayirli A, and Sahin K
- Subjects
- Animals, Catalase analysis, Female, Glutathione Peroxidase analysis, Malondialdehyde blood, Plant Extracts administration & dosage, Random Allocation, Superoxide Dismutase analysis, Vitamin A blood, Vitamin E blood, Cirsium metabolism, Coturnix metabolism, Dietary Supplements standards, Eggs analysis, Plant Extracts pharmacology
- Abstract
1. The herb Creeping Thistle, Cirsium arvense (C. arvense), has been used in folk medicine due to its antioxidant and anti-inflammatory effects. 2. The aim of this study was to investigate the effects of dietary C. arvense extract supplementation on performance, egg quality, nutrient digestibility and antioxidant status in quail. 3. Quails (n = 150) were allocated randomly to one of the three dietary treatments: basal diet and basal diet enriched with 100 and 200 mg C. arvense extract per kg diet. 4. Dietary enrichment with C. arvense extract altered neither performance and egg quality parameters nor nutrient digestibility. Although there were no changes in concentrations of vitamin A and E in serum, liver and egg yolk, supplemental C. arvense extract decreased MDA concentrations in serum, liver and egg yolk by 39.3, 40.5 and 51.5%, respectively, in a dose-response manner. As supplemental C. arvense extract increased to 200 mg/kg, the activity of hepatic SOD, CAT and GSH-Px increased by 14.5, 17.4 and 35.5%, respectively. 5. Addition of C. arvense extract up to 200 mg to per kg diet enhanced antioxidant status in laying quail and their eggs, without affecting performance and other egg quality parameters. 6. Future studies are needed to elucidate the mechanism behind the antioxidant effects of C. arvense extract.
- Published
- 2013
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- View/download PDF
35. The anticancer activity of propolis.
- Author
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Sawicka D, Car H, Borawska MH, and Nikliński J
- Subjects
- Animals, Antineoplastic Agents, Phytogenic chemistry, Apoptosis drug effects, Cell Cycle Checkpoints drug effects, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Humans, Phytotherapy, Propolis chemistry, Structure-Activity Relationship, Antineoplastic Agents, Phytogenic pharmacology, Neoplasms drug therapy, Neoplasms pathology, Propolis pharmacology
- Abstract
Propolis and its compounds have been the subject of many studies due to their antimicrobial and antiinflammatory activity; however, it is now known that they also possess antitumor properties. This review aims to summarize the results of studies on the mechanism of activity of propolis and its active compounds such as CAPE and chrysin in the apoptotic process, and their influence on the proliferation of cancer cells. Our review shows that propolis and its presented compounds induce apoptosis pathways in cancer cells. The antiproliferative effects of propolis, CAPE or chrysin in cancer cells are the result of the suppression of complexes of cyclins, as well as cell cycle arrest. The results of in vitro and in vivo studies suggest that propolis, CAPE and chrysin may inhibit tumor cell progression and may be useful as potential chemotherapeutic or chemopreventive anticancer drugs.
- Published
- 2012
- Full Text
- View/download PDF
36. Diet and the content of selenium and lead in patients with abdominal aortic aneurysm.
- Author
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Socha K, Borawska MH, Gacko M, and Guzowski A
- Subjects
- Adult, Aged, Aged, 80 and over, Aortic Aneurysm, Abdominal blood, Case-Control Studies, Female, Humans, Linear Models, Male, Middle Aged, Poland, Risk Assessment, Risk Factors, Spectrophotometry, Atomic, Surveys and Questionnaires, Aorta chemistry, Aortic Aneurysm, Abdominal etiology, Diet adverse effects, Lead blood, Selenium blood, Smoking adverse effects
- Abstract
Background: To evaluate the content of selenium (Se) and lead (Pb) and the influence of dietary habits and smoking in patients with abdominal aortic aneurysm (AAA)., Patients and Methods: Forty-nine patients with AAA prior to surgical procedures aged 42 - 81 years and a control group of 22 healthy volunteers aged 31 - 72 years and 17 aortic wall samples from deceased were included in the study. Food-frequency questionnaires were implemented in AAA patients to collect the dietary data. Se and Pb concentrations in the serum and blood, respectively, and in arterial wall and parietal thrombus samples were determined by the atomic absorption spectrometry method., Results: The mean Se level in serum of patients with AAA (60.37 ± 21.2 cm/L) was significantly (p < 0.008) lower than in healthy volunteers (75.87 ± 22.4 cm/L). We observed a significant correlation (r = 0.69, p < 0.0001) between the content of Se in serum and the parietal thrombus of examined patients. Se concentration in aortic wall was inversely correlated to the concentration of Pb (r = - 0.38, p < 0.02). We observed significantly lower (p < 0.05) concentrations of Se (39.14 ± 37.1 cm/g) and significantly higher (p < 0.05) concentrations of Pb (202.69 ± 180.6 cm/g) in aortic wall samples of smoking patients than in non-smoking patients (77.56 ± 70.0 cm/g, 73.09 ± 49.8 cm/g; respectively)., Conclusions: Se serum level is lower in patients with AAA than in healthy volunteers. In aortic wall, Se concentration is inversely correlated with Pb concentration. Dietary habits and smoking have an influence on the Se and Pb status in patients with AAA.
- Published
- 2011
- Full Text
- View/download PDF
37. Enhancement of antibacterial effects of extracts from Cirsium species using sodium picolinate and estimation of their toxicity.
- Author
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Borawska MH, Czechowska SK, Markiewicz R, Socha K, Nazaruk J, Pałka J, and Isidorov VA
- Subjects
- Anti-Bacterial Agents analysis, Cirsium toxicity, Drug Synergism, Fibroblasts drug effects, Flowers chemistry, Humans, Microbial Sensitivity Tests, Plant Extracts chemistry, Plant Extracts toxicity, Plant Leaves chemistry, Plants, Medicinal chemistry, Plants, Medicinal toxicity, Sodium Benzoate pharmacology, Anti-Bacterial Agents pharmacology, Cirsium chemistry, Iron Chelating Agents pharmacology, Picolinic Acids pharmacology, Plant Extracts pharmacology
- Abstract
In this study, antimicrobial properties and toxicity of extracts from Cirsium spp.: Cirsium arvense, C. oleraceum, C. palustre, C. rivulare and C. vulgare in combination with sodium picolinate (PS) or sodium benzoate (BS), were investigated. Three micro-organisms were used: Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. Minimum inhibitory concentration (MIC) of extracts was found at 1.56-50.0 mg mL(-1). Unlike the case of BS, adding PS to extracts from flowers of C. palustre and C. arvense enhanced their antimicrobial effect on S. aureus (MIC from 6.25-12.5 mg mL(-1) to 1.25-5.0 mg mL(-1)). An MTT test was used to study toxicity effects. The extracts from C. palustre or C. arvense mixed with PS had a concentration-dependent, slightly cytotoxic or stimulating effect on the viability of normal human skin fibroblasts. The total phenolic content (TPC) of samples varied from 44 to 178 mg gallic acid equivalent per 1 g of extract. The highest TPC was observed in C. palustre (l) and C. oleraceum (f). Our results did not show any correlation between antimicrobial activities and TPC. Cirsium palustre (f) and C. arvense (f) extracts were analysed by gas chromatography/mass spectrometry (GC/MS). About 30 compounds were found to be present in extracts from two Cirsium species in amounts of not less than 0.2% of TIC.
- Published
- 2010
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38. Cell viability of normal human skin fibroblast and fibroblasts derived from granulation tissue: effects of nutraceuticals.
- Author
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Borawska MH, Czechowska SK, Markiewicz R, Hayirli A, Olszewska E, and Sahin K
- Subjects
- Analysis of Variance, Camellia sinensis, Carotenoids pharmacology, Catechin analogs & derivatives, Catechin pharmacology, Cells, Cultured, Cholesteatoma, Middle Ear, Dietary Supplements, Dimethyl Sulfoxide pharmacology, Furans pharmacology, Genistein pharmacology, Granulation Tissue cytology, Humans, Lycopene, Skin cytology, Solvents pharmacology, Antioxidants pharmacology, Cell Survival drug effects, Fibroblasts drug effects, Granulation Tissue drug effects, Plant Extracts pharmacology, Skin drug effects
- Abstract
The effects of lycopene, genistein, and epigallocatechin-3-gallate (EGCG) on cell viability were tested in vitro using a normal human skin fibroblast (NHSF) cell line (CRL-1474) and granulation tissue fibroblasts (GTFs) obtained from a patient with middle ear cholesteatoma. Cell cultures were added with lycopene (1, 5, and 10 microM), genistein (1, 5, 10, 25, and 50 microM), and EGCG (1, 5, 10, 25, and 50 microM) and their respective control cultures were established by adding 5 mL/L tetrahydrofuran (THF), 5 mL/L dimethyl sulfoxide (DMSO), and 5 mL/L DMSO. A colorimetric assay was employed for determining cell viability using thiazolyl blue tetrazolium bromide. Cell viability was expressed as a percentage of the control. Data were analyzed using two-way analysis of variance separately for each compound. Lycopene addition decreased viability of NHSFs and GTFs compared with THF addition (64.1%, 60.5%, and 100%, respectively, P < .0001). Genistein addition also increased viability of both NHSFs and GTFs compared with DMSO addition (P < .02). Increasing EGCG concentration tended to cause a linear increase in viability of NHSFs but did not alter viability of GTFs (P < .10). Our data suggest that genistein and EGCG but not lycopene could help maintaining or improving skin health through enhancing viability of skin fibroblasts.
- Published
- 2009
- Full Text
- View/download PDF
39. TNF-alpha and sICAM-1 in intracranial aneurismal rupture.
- Author
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Witkowska AM, Borawska MH, Socha K, Kochanowicz J, Mariak Z, and Konopka M
- Subjects
- Adult, Aged, Aneurysm, Ruptured blood, Brain blood supply, Brain pathology, Cerebrovascular Circulation physiology, Female, Glasgow Coma Scale, Glasgow Outcome Scale, Humans, Intracranial Aneurysm blood, Male, Middle Aged, Prognosis, Subarachnoid Hemorrhage blood, Subarachnoid Hemorrhage etiology, Aneurysm, Ruptured complications, Intercellular Adhesion Molecule-1 blood, Intracranial Aneurysm complications, Subarachnoid Hemorrhage diagnosis, Tumor Necrosis Factor-alpha blood
- Abstract
Introduction: Subarachnoidal hemorrhage (SAH) occurring after aneurysmal rupture produces an inflammatory response in the cerebral circulation. Tumor necrosis factor (TNF)-alpha is a major cytokine in this process. Adhesion molecules provide information on inflammatory reactions taking place in the walls of blood vessels. Clinical evidence suggests a role of soluble intercellular adhesion molecule (sICAM)-1 in early hemorrhagic events. This study aimed to evaluate the implementation of early TNF-alpha and sICAM-1 serum measurement for the prognosis of patient outcome after intracranial aneurysmal rupture., Materials and Methods: The study consisted of 27 patients with a diagnosis of intracranial aneurysm. SAH was evaluated on admission according to the Fisher scale, patients' consciousness with the Glasgow Coma Scale, clinical grading with the Hunt and Hess scale, and clinical outcome with the Glasgow Outcome Scale (GOS). Blood samples were drawn within 72 h after arrival at the emergency room. Serum concentrations of TNF-alpha and sICAM-1 were assayed with the ELISA method., Results: The initial serum TNF-alpha concentration in the aneurysmal patients was low and did not correlate with radiological and clinical scores. The serum sICAM-1 level positively correlated with the severity of bleeding assessed by the Fisher scale and negatively with the patient's scoring in the GOS., Conclusions: This study demonstrated the absence of a systemic TNF-alpha-mediated inflammatory response at the onset of subarachnoid hemorrhage. Early measurement of serum sICAM-1 levels offers a potential prognostic value in the assessment of patients' outcome after brain aneurysmal rupture.
- Published
- 2009
- Full Text
- View/download PDF
40. The effects of diet on selenium concentration in serum in patients with cancer.
- Author
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Borawska MH, Socha K, Łazarczyk B, Czyzewska E, Markiewicz R, and Darewicz B
- Subjects
- Adult, Aged, Aged, 80 and over, Body Mass Index, Diet Surveys, Female, Humans, Laryngeal Neoplasms chemistry, Male, Middle Aged, Poland, Regression Analysis, Risk Factors, Selenium analysis, Selenium deficiency, Urologic Neoplasms chemistry, Diet, Feeding Behavior, Food, Laryngeal Neoplasms blood, Nutritional Status, Selenium blood, Urologic Neoplasms blood
- Abstract
The aim of this study was to determine serum levels of selenium (Se) in patients with larynx and urinary system cancers. We also estimated the influence of dietary habits on Se status in examined patients. The mean content of Se in serum of patients with urinary system cancer (48.94 +/- 16.3 mu/l) and larynx cancer (51.00 +/- 18.6 mu/l) was lower than the mean content of Se in the control group (68.25 +/- 15.6 mircog/l; P = 0.000006 or 64.03 +/- 16.8 microg/l; P = 0.0112, respectively). In tissue only, the mean level of Se in patients with kidney cancer (75.37 +/- 40.3 mircog/l) was lower to compare with the dead body control group (220.68 +/- 83.6 microg/l). We have observed the correlation between the content of Se in serum and tissue (r = 0.297; P = 0.002). Patients with studied cancers have deficiency of Se in serum and kidney tissue, and it depends on the diet in about 30%. Frequent consumption of eggs, ham, and wine has the biggest influence on the content of Se in serum of patients in Poland, whereas frequent consumption of pulses, eggs, bacon, and lard is connected with the content of Se in tissue.
- Published
- 2009
- Full Text
- View/download PDF
41. Polyphenolic compounds and in vitro antimicrobial and antioxidant activity of aqueous extracts from leaves of some Cirsium species.
- Author
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Nazaruk J, Czechowska SK, Markiewicz R, and Borawska MH
- Subjects
- Anti-Infective Agents chemistry, Antioxidants chemistry, Bacillus subtilis drug effects, Candida albicans drug effects, Flavonoids chemistry, Gallic Acid, Microbial Sensitivity Tests, Phenols chemistry, Plant Leaves chemistry, Polyphenols, Pseudomonas aeruginosa drug effects, Staphylococcus aureus drug effects, Tannins analysis, Anti-Infective Agents isolation & purification, Anti-Infective Agents pharmacology, Antioxidants isolation & purification, Antioxidants pharmacology, Cirsium chemistry, Flavonoids isolation & purification, Flavonoids pharmacology, Phenols isolation & purification, Phenols pharmacology, Plants, Medicinal chemistry
- Abstract
Crude aqueous extracts from leaves of Cirsium arvense, C. oleraceum, C. palustre, C. rivulare and C. vulgare were investigated. The content of tannins in mentioned sources, determined by the weight method with hide powder, varied between 1 and 7.63%. Total phenolic content, analysed by using Folin-Ciocalteau's method, ranged between 54 and 96 mg g(-1), was expressed as milligrams of gallic acid per gram of dry extract. Phenolic acids were identified by HPLC method. Antimicrobial activity of those extracts was examined. Cirsium palustre extract was the most active against investigated microorganisms. It was observed that the content of small-molecular phenolic compounds had greater influence on the activity of extracts than tannins. The total antioxidant activity indicated by radical cation 2,2'-azini-di-(3-ethylbenzthiazoline sulphonate) ABTS(*+), expressed as total antioxidant status (TAS) ranged from 2.31 to 2.78 mM L(-1).
- Published
- 2008
- Full Text
- View/download PDF
42. [Zinc and copper in diets of people living in the social nursing home in Białystok].
- Author
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Markiewicz R, Socha K, Borawska MH, and Gutowska A
- Subjects
- Food Analysis methods, Food Services organization & administration, Humans, Nursing Homes organization & administration, Nutritional Physiological Phenomena, Nutritional Requirements, Nutritive Value, Poland epidemiology, Copper analysis, Diet statistics & numerical data, Food Analysis statistics & numerical data, Zinc analysis
- Abstract
The aim of the work was the estimation zinc and copper content in collected diets of people from social nursing home in Białystok by analytical and calculating method. The content of microelements in diets was estimated by analytical method ASA and using computer program Food 2. Diets in a winter period had statistically significantly less zinc, on the average 3.904 +/- 0.62 than in the summer time, 4.684 +/- 0.78 mg/kg. The average daily intake of zinc estimated in analytical method in the winter diet was 10.037 +/- 1.59 and it was statistically significantly lower than in the summer time, 12.303 +/- 2.17 mg/person/day. Theoretical method gave higher results. The average content of copper in diets in winter time (0.380 +/- 0.07 mg/kg) was statistically significantly lower than in the summer time (0.454 +/- 0.06 mg/kg). Daily intake of copper from diet was statistically significant lower in winter (0.975 +/- 0.15) comparing to the summer time (1.187 +/- 0.12 mg/person/day). Theoretical consumption of copper was 1.650 +/- 0.47 in winter and 1.464 +/- 0.25 mg/person/day in the summer time. The analyzed diets were properly balanced taking into account the energetic and zinc values but did not achieve the recommended level for copper.
- Published
- 2008
43. Relationship among TNF-alpha, sICAM-1, and selenium in presurgical patients with abdominal aortic aneurysms.
- Author
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Witkowska AM, Borawska MH, and Gacko M
- Subjects
- Adult, Aged, Aged, 80 and over, Aortic Aneurysm, Abdominal surgery, Female, Humans, Male, Middle Aged, Preoperative Care, Aortic Aneurysm, Abdominal blood, Intercellular Adhesion Molecule-1 blood, Selenium blood, Tumor Necrosis Factor-alpha metabolism
- Abstract
The purpose of our study was to estimate the utility of tumor necrosis factor (TNF), soluble intercellular adhesion molecule-1 (sICAM-1), and selenium (Se) measurements as subsidiary parameters for the diagnosis and differentiation of large aortic abdominal aneurysms (AAAs). The TNF and sICAM-1 levels in AAAs were significantly higher than in the controls. On the contrary, the Se level in AAAs was slightly lower; however, the difference was not significant. sICAM-1 did not correlate with TNF or Se as well as with any physical parameter. TNF was related to body mass index (r = 0.375). A negative correlation was found for Se and the aneurysm's diameter (r = -0.382), especially for the height-adjusted diameter (r = -0.418). The results of the present study do not allow one to count TNF and sICAM-1 as markers of AAA advancement. It was shown for the first time that the level of serum Se is low in ruptured aneurysms, but not in the unruptured AAAs.
- Published
- 2006
- Full Text
- View/download PDF
44. Soluble intercellular adhesion molecule-1 (sICAM-1): an overview.
- Author
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Witkowska AM and Borawska MH
- Subjects
- Animals, Arteriosclerosis immunology, Autoimmune Diseases immunology, Cell Adhesion immunology, Cytokines immunology, Humans, Neoplasms immunology, Virus Diseases immunology, Cell Movement immunology, Endothelial Cells immunology, Intercellular Adhesion Molecule-1 immunology, Leukocytes immunology, Lymphocyte Function-Associated Antigen-1 immunology
- Abstract
Soluble intercellular adhesion molecule-1 (sICAM-1) represents a circulating form of ICAM-1 that is constitutively expressed or is inducible on the cell surface of different cell lines. It serves as a counter-receptor for the lymphocyte function-associated antigen (LFA-1). Interaction between ICAM-1, present on endothelial cells, and LFA-1 facilitates leukocyte adhesion and migration across the endothelium. ICAM-1 and its circulating form have been implicated in the development of any number of diseases.
- Published
- 2004
45. Influence of dietary habits on serum selenium concentration.
- Author
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Borawska MH, Witkowska AM, Hukałowicz K, and Markiewicz R
- Subjects
- Alcohol Drinking blood, Female, Food Analysis, Glutathione Peroxidase metabolism, Honey analysis, Humans, Male, Meat Products analysis, Middle Aged, Regression Analysis, Selenium deficiency, Smoking blood, Spectrophotometry methods, Surveys and Questionnaires, Tea chemistry, Feeding Behavior, Selenium blood
- Abstract
Background: A serum selenium concentration of 70 microg/l is considered to be sufficient for glutathione peroxidase (GSHPx) activity, a selenium-dependent enzyme reflecting the body selenium status. The purpose of the study was to assess which foods or food groups best affect serum selenium in subjects with selenium concentrations below or above 70 microg/l., Methods: A food frequency questionnaire was employed to describe the dietary habits of 129 subjects. Serum selenium concentrations were determined using the electrothermal absorption spectrometry (ETAAS) technique following serum dilution., Results: Multiple regression analysis revealed that consumption of ham, honey and tea was positively associated with the selenium concentration in the sera of selenium-inadequate subjects. In selenium-inadequate subjects, alcohol consumption may be responsible for selenium losses. Unlike the selenium-inadequate subjects, the selenium concentrations in the sera of the selenium-adequate subjects were positively correlated with the frequencies of wholegrain bread consumption and processed fruit consumption. In all the groups tested, the serum selenium concentration was not affected by cigarette smoking., Conclusions: The serum selenium concentration is influenced by dietary habits, but not by cigarette smoking. Frequent consumption of ham, tea and honey may be effective to improve the selenium concentration in the sera of selenium-inadequate subjects., (Copyright 2004 S. Karger AG, Basel)
- Published
- 2004
- Full Text
- View/download PDF
46. A study on soluble intercellular adhesion molecule-1 and selenium in patients with rheumatoid arthritis complicated by vasculitis.
- Author
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Witkowska AM, Kuryliszyn-Moskal A, Borawska MH, Hukałowicz K, and Markiewicz R
- Subjects
- Adult, Aged, Biomarkers analysis, Case-Control Studies, Cohort Studies, Enzyme-Linked Immunosorbent Assay, Female, Humans, Intercellular Adhesion Molecule-1 analysis, Male, Middle Aged, Probability, Prognosis, Reference Values, Selenium analysis, Sensitivity and Specificity, Severity of Illness Index, Solubility, Statistics, Nonparametric, Arthritis, Rheumatoid complications, Arthritis, Rheumatoid diagnosis, Intercellular Adhesion Molecule-1 metabolism, Selenium metabolism, Vasculitis complications, Vasculitis diagnosis
- Abstract
Clinical manifestations of vasculitis, as a complication of rheumatoid arthritis (RA), can be postulated as a consequence of immune response abnormalities and endothelial cell dysfunction. In this study we searched for the relationship between the extent of vascular involvement and either serum sICAM-1 or selenium concentrations. We also explored the possible interaction of serum selenium with sICAM-1 to provide a greater understanding of their role in rheumatoid vasculitis (RV). For the study, we measured the serum titers of sICAM-1 using an ELISA assay and the serum selenium concentrations using the ETAAS method in 37 women suffering from RA and 18 normal women serving as controls. All the RA patients were evaluated by extensive clinical, laboratory and capillaroscopic studies. In all patients with extra-articular manifestations, severe or moderate changes in nailfold capillaroscopy were found. Serum sICAM-1 titers in RA patients with mild vasculitis on nailfold capillaroscopy did not differ significantly from those of the healthy subjects, whereas a higher sICAM-1 level seemed to reflect the more intensive vascular changes in capillaroscopy. These data suggest that sICAM-1 serum levels may reflect the extent of the microvascular involvement in RA patients. Compared with controls, all the RA patients had markedly lower serum selenium concentrations, irrespective of the degree of the capillaroscopic vascular changes. A significant inverse correlation between sICAM-1 and selenium was found in the controls (r = -0.54; P<0.02). By contrast, no correlation was noted in RA patients (r=0.10, P not significant). This suggests that the sICAM-1 shedding in RV does not appear to be influenced by selenium, presumably owing to its low serum concentration.
- Published
- 2003
- Full Text
- View/download PDF
47. Radioactivity of honeybee honey.
- Author
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Borawska MH, Kapała J, Hukałowicz K, and Markiewicz R
- Subjects
- Animals, Environmental Monitoring, Ukraine, Bees, Cesium Radioisotopes analysis, Food Contamination, Honey, Radioactive Hazard Release
- Published
- 2000
- Full Text
- View/download PDF
48. [The role of dietary fiber and its preparations in the protection and treatment of overweight].
- Author
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Witkowska A and Borawska MH
- Subjects
- Humans, Dietary Fiber metabolism, Obesity prevention & control, Obesity therapy
- Abstract
Optimal amounts of dietary fibre in the diet are regarded as a protective factor against several health disorders such as some alimentary tract diseases, atherosclerosis and coronary heart disease. It is considered that the dietary fibre may help reduce body weight. The preparations of dietary fibre slow gastric emptying and decrease the appetite. However, the reduction of body weight with the application of high fibre diets, but without a change in the eating habits, is not significant.
- Published
- 1999
49. Effects of vasopressin and analogue [d(CH2)1(5), Tyr (Me)2, Val4, delta 3Pro7)] AVP on learning and memory in rats chronically treated with ethanol.
- Author
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Car H, Borawska MH, and Wiśniewski K
- Subjects
- Animals, Arginine Vasopressin administration & dosage, Arginine Vasopressin pharmacology, Avoidance Learning drug effects, Extinction, Psychological drug effects, Injections, Intraventricular, Male, Rats, Rats, Wistar, Arginine Vasopressin analogs & derivatives, Ethanol pharmacology, Learning drug effects, Memory drug effects
- Abstract
Rats with impaired active and passive avoidance induced by chronic administration of ethanol were studied. Vasopressin (AVP) and analogue (d(CH2)1(5), Tyr (Me)2, Val4, delta 3Pro7] AVP (icv, 2 micrograms) eliminated the toxic effect of ethanol, and analogue AVP improved retrieval of passive avoidance situation in both control and postalcohol groups of rats. Chronically administered ethanol markedly depressed the ability to learn. AVP administered icv markedly delayed extinction of conditioned avoidance situation in both groups of rats, and did not influence acquisition in this test. Analogue AVP has no influence on extinction and acquisition in the control and post-alcohol groups of rats.
- Published
- 1994
50. Effect of vasopressin analog [d(CH2)1(5),Tyr(Me)2]AVP on learning and memory in rats chronically treated with ethanol.
- Author
-
Car H, Borawska MH, and Wiśniewski K
- Subjects
- Animals, Arginine Vasopressin pharmacology, Avoidance Learning drug effects, Exploratory Behavior drug effects, Injections, Intraventricular, Male, Motor Activity drug effects, Rats, Rats, Wistar, Arginine Vasopressin analogs & derivatives, Arginine Vasopressin antagonists & inhibitors, Ethanol pharmacology, Learning drug effects, Memory drug effects
- Abstract
The effect of single 2 micrograms dose of vasopressin (AVP) and analog [d(CH2)1(5),Tyr(Me)2]AVP on processes of retrieval, acquisition and consolidation of conditioned reflexes in rats chronically alcoholized was studied. Long term (9 weeks) ethanol intoxication profoundly impaired learning and memory processes in all tests used. The AVP analog [d(CH2)1(5), Tyr(Me)2]AVP facilitated retrieval of passive avoidance, improved consolidation of active avoidance of rats previously treated with alcohol, but did not affect the acquisition of active avoidance. [d(CH2)1(5), Tyr(Me)2]AVP did not affect the motor and exploratory activity of rats in open field.
- Published
- 1993
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