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32 results on '"Bornard, Isabelle"'

9. Flashes of UV-C light are perceived by UVR8, the photoreceptor of UV-B light

Author

Aarrouf Jawad, Hdech Douae Ben, Diot Alice, Bornard Isabelle, Félicie Lauri, Urban Laurent, Démarche intégrée pour l'obtention d'aliments de qualité (UMR QualiSud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Avignon Université (AU)-Université de La Réunion (UR)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Unité de Pathologie Végétale (PV), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French Ministry of Agriculture and Food (CASDAR project Oï dUV), and Heighten Science Publications Inc. (HSPI)

Subjects
stimulating plant defenses, plant-pathogen interaction, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Plant pathology, Ultraviolet light sources, photoreceptor, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract

The confocal microscope was funded by the European Regional Development Fund, the French government, the Sud Provence-Alpes-Côte d’Azur Region, the Departmental Council of Vaucluse and the Urban Community of Avignon.; International audience; Light is an important regulator of plant morphogenesis and plant-pathogen interactions via specific photoreceptors and signaling pathways. Besides visible light, other electromagnetic radiations may play roles, notably ultraviolet (UV) light. The UV part of the electromagnetic spectrum includes UV-A (315 nm - 400 nm), UV-B (280 nm - 315 nm) and UV-C radiations (200 nm - 280 nm). UV-B and UV-C have been reported to increase plant resistance to plant pathogens after the UV perception and signaling stages. The perception of UV-B radiation is achieved by the dimer protein UVR8 (UV RESISTANCE LOCUS 8). Even though the action spectrum of this photoreceptor overlaps in the UV-C domain, it has never been formally demonstrated that UVR8 could also act as a photoreceptor of UV-C light. We provide here original observations showing that UVR8 can indeed perceive UV-C light provided that the latter is in the form of flashes (1s) and not continuous illuminations (the 60s). Our observations also show that the response of UVR8 to flashes of UV-C light is dose-dependent. They could explain why flashes of UV-C light are more effective for stimulating plant defenses than continuous illuminations for the same amount of energy delivered to plants (J/m2). Eventually, our observations support ongoing trials that aim at using UV-C light as an environmental-friendly plant resistance inducer in field conditions.

Published
2022

19. Differential involvement of the five RNA helicases in adaptation of Bacillus cereus ATCC 14579 to low growth temperatures

Author

Pandiani, Franck, Chamot, Stephanie, Nguyen-the, Chritophe, Broussolle, Veronique, Brillard, Julien, Bornard, Isabelle, and Michaud, Caroline

Subjects
RNA -- Analysis, Helicases -- Analysis, Bacillus cereus -- Physiological aspects, Phenotype -- Analysis, Biological sciences
Abstract

The role of the five putative RNA helicases present in the genome of Bacillus cereus ATCC 14579 in its adaptation at low temperature, close to the growth limit is examined. The studies have shown that cold phenotype is restored with the cshA gene for the [DELTA]cshA strain and partially for the [DELTA]cshB strain but not for the [DELTA]cshC strain, which has shown different functions at low temperature.

Published
2010

21. Identification of Lychnis ringspot virus in Mentha piperita in Italy by Next Generation Sequencing technology

Author

Parrella, Giuseppe, Bellardi, M.G., Bornard, Isabelle, Marais, Armelle, Candresse, Thierry, Institute for the Sustainable Plant Protection, Partenaires INRAE, Department of Agricultural Sciences, Alma Mater Studiorum University of Bologna (UNIBO), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1

Subjects
lychnis ringspot virus, virus phytopathogène, Virologie, Microbiology and Parasitology, Microbiologie et Parasitologie, italie, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Virology, santé des plantes, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, identification, mentha piperita, virologie végétale, pathologie végétale, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

22. Coniothyrium minitans parasite les sclérotes de Sclerotinia minor

Author

Bornard, Isabelle, Boulon, Jean-Philippe, Bardin, Marc, and Troulet, Claire

Subjects
champignon à sclérote, pycnide, résistance aux maladies, microscopie à balayage électronique, champignon phytopathogène, protection biologique, hyperparasitisme, culture légumière de plein champ, maladie des plantes, étude in vitro, Agricultural sciences, reproduction asexuée, laitue, sclerotinia minor, méthode de lutte, Coniothyrium minitans, champignon du sol, champignon ascomycete, épidémiologie végétale, agent de lutte biologique, Sciences agricoles
Published
2014

26. Improved microwave steam distillation apparatus for isolation of essential oils: Comparison with conventional steam distillation

Author

Sahraoui, Naima, Vian, Maryline, Bornard, Isabelle, Boutekedjiret, Chahrazed, Chemat, Farid, Ecole Nationale Polytechnique d'Algérie, Partenaires INRAE, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), and Centre de Recherche en Analyses Physico-Chimiques

Subjects
ESSENTIAL OIL, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, LAVANDE, food and beverages, SAMPLE PREPARATION, MICROWAVE, STEAM DISTILLATION, complex mixtures, METHODOLOGIE
Abstract

International audience; Steam distillation (SD) is routinely used by analysts for the isolation of essential oils from herbs, flowers and spices prior to gas chromatographic analysis. In this work, a new process design and operation for an improved microwave steam distillation (MSD) of essential oils from aromatic natural products was developed. To demonstrate its feasibility, MSD was compared with the conventional technique, SD, for the analysis of volatile compounds from dry lavender flowers (Lavandula angustifolia Mill., Lamiaceae). Essential oils isolated by MSD were quantitatively (yield) and qualitatively (aromatic profile) similar to those obtained by SD, but MSD was better than SD in terms of rapidity (6 min versus 30 min for lavender flowers), thereby allowing substantial savings of costs in terms of time and energy. Lavender flowers treated by MSD and SD were observed by scanning electron microscopy. Micrographs provide evidence of more rapid opening of essential oil glands treated by MSD, in contrast to conventional SD

Published
2008

27. First report of tobacco rattle virus in Aquilegia sp. in France

Author

CARDIN, Loïc, Onesto, Jean-Paul, Bornard, Isabelle, Moury, Benoît, Unité Recherches Intégrées en Horticulture (URIH), Institut National de la Recherche Agronomique (INRA), and Unité de Pathologie Végétale (PV)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, virus phytopathogène, TOBACCO RATTLE VIRUS, Phytopathology and phytopharmacy, TOBRAVIRUS, TRV, RT-PCR, RANUNCULACEAE, AQUILEGIA, ANCOLIE, Virologie, microscopie électronique, virus, Phytopathologie et phytopharmacie, Agricultural sciences, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Virology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Sciences agricoles, ComputingMilieux_MISCELLANEOUS, technique elisa
Abstract

International audience

Published
2008

32. Sporulation Temperature Reveals a Requirement for CotE in the Assembly of both the Coat and Exosporium Layers of Bacillus cereus Spores.

Author

Bressuire-Isoard C, Bornard I, Henriques AO, Carlin F, and Broussolle V

Subjects
Bacillus cereus growth & development, Bacterial Proteins analysis, Bacterial Proteins genetics, Gene Expression Regulation, Bacterial, Hydrogen Peroxide metabolism, Hydrophobic and Hydrophilic Interactions, Inosine metabolism, Muramidase metabolism, Spores, Bacterial chemistry, Spores, Bacterial genetics, Spores, Bacterial ultrastructure, Temperature, Bacillus cereus genetics, Bacillus cereus physiology, Bacterial Proteins physiology, Spores, Bacterial growth & development
Abstract

The Bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. We investigated the interplay between the sporulation temperature and the CotE morphogenetic protein in the assembly of the surface layers of B. cereus ATCC 14579 spores and on the resulting spore properties. The cotE deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°C and at the optimal growth temperature of 37°C. Transmission electron microscopy revealed that ΔcotE spores had a fragmented and detached exosporium when formed at 37°C. However, when produced at 20°C, ΔcotE spores showed defects in both coat and exosporium attachment and were susceptible to lysozyme and mutanolysin. Thus, CotE has a role in the assembly of both the coat and exosporium, which is more important during sporulation at 20°C. CotE was more represented in extracts from spores formed at 20°C than at 37°C, suggesting that increased synthesis of the protein is required to maintain proper assembly of spore surface layers at the former temperature. ΔcotE spores formed at either sporulation temperature were impaired in inosine-triggered germination and resistance to UV-C and H2O2 and were less hydrophobic than wild-type (WT) spores but had a higher resistance to wet heat. While underscoring the role of CotE in the assembly of B. cereus spore surface layers, our study also suggests a contribution of the protein to functional properties of additional spore structures. Moreover, it also suggests a complex relationship between the function of a spore morphogenetic protein and environmental factors such as the temperature during spore formation., (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)

Published
2015
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