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28 results on '"Borowczyk, Julia"'

14. Keratinocyte-specific ablation of Mcpip1 impairs skin integrity and promotes local and systemic inflammation

Author

Konieczny, Piotr, primary, Lichawska-Cieslar, Agata, additional, Kwiecinska, Patrycja, additional, Cichy, Joanna, additional, Pietrzycka, Roza, additional, Szukala, Weronika, additional, Declercq, Wim, additional, Devos, Michael, additional, Paziewska, Agnieszka, additional, Rumienczyk, Izabela, additional, Kulecka, Maria, additional, Mikula, Michal, additional, Fu, Mingui, additional, Borowczyk, Julia, additional, Santamaria-Babí, Luis F, additional, and Jura, Jolanta, additional

Published
2019
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16. Effect of silver nanoparticles on human primary keratinocytes

Author

Szmyd, Radoslaw, primary, Goralczyk, Anna Grazyna, additional, Skalniak, Lukasz, additional, Cierniak, Agnieszka, additional, Lipert, Barbara, additional, Filon, Francesca Larese, additional, Crosera, Matteo, additional, Borowczyk, Julia, additional, Laczna, Eliza, additional, Drukala, Justyna, additional, Klein, Andrzej, additional, and Jura, Jolanta, additional

Published
2016
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17. Fenofibrate enhances barrier function of endothelial continuum within the metastatic niche of prostate cancer cells

Author

Piwowarczyk, Katarzyna, primary, Wybieralska, Ewa, additional, Baran, Jarosław, additional, Borowczyk, Julia, additional, Rybak, Paulina, additional, Kosińska, Milena, additional, Włodarczyk, Anna Julia, additional, Michalik, Marta, additional, Siedlar, Maciej, additional, Madeja, Zbigniew, additional, Dobrucki, Jerzy, additional, Reiss, Krzysztof, additional, and Czyż, Jarosław, additional

Published
2014
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19. Effects triggered by platinum nanoparticles on primary keratinocytes

Author

Konieczny,Piotr, Goralczyk,Anna Grazyna, Szmyd,Radoslaw, Skalniak,Lukasz, Koziel,Joanna, Filon,Francesca Larese, Crosera,Matteo, Cierniak,Agnieszka, Zuba-Surma,Ewa K, Borowczyk,Julia, Laczna,Eliza, Drukala,Justyna, Pyza,Elzbieta, Semik,Danuta, Woznicka,Olga, Klein,Andrzej, Jura,Jolanta, Konieczny,Piotr, Goralczyk,Anna Grazyna, Szmyd,Radoslaw, Skalniak,Lukasz, Koziel,Joanna, Filon,Francesca Larese, Crosera,Matteo, Cierniak,Agnieszka, Zuba-Surma,Ewa K, Borowczyk,Julia, Laczna,Eliza, Drukala,Justyna, Pyza,Elzbieta, Semik,Danuta, Woznicka,Olga, Klein,Andrzej, and Jura,Jolanta

Abstract

Piotr Konieczny,1,* Anna Grazyna Goralczyk,1,* Radoslaw Szmyd,1,* Lukasz Skalniak,1,* Joanna Koziel,2 Francesca Larese Filon,3 Matteo Crosera,4 Agnieszka Cierniak,1 Ewa K Zuba-Surma,5 Julia Borowczyk,5 Eliza Laczna,5 Justyna Drukala,5 Elzbieta Pyza,6 Danuta Semik,6 Olga Woznicka,6 Andrzej Klein,1 Jolanta Jura11Department of General Biochemistry, 2Department of Microbiology, Jagiellonian University, Kraków, Poland; 3Department of Public Health Sciences, 4Department of Chemical and Pharmaceutical Sciences, University of Trieste, Trieste, Italy; 5Department of Cell Biology, 6Department of Cell Biology and Imaging, Jagiellonian University, Kraków, Poland*These authors equally contributed to this workAbstract: The platinum (Pt)-group elements (PGEs) represent a new kind of environmental pollutant and a new hazard for human health. Since their introduction as vehicle-exhaust catalysts, their emissions into the environment have grown considerably compared with their low natural concentration in the earth crust. PGE emissions from vehicle catalysts can be also in the form of nanometer-sized particles (Pt nanoparticles [PtNPs]). These elements, both in their metallic form or as ions solubilized in biological media, are now recognized as potent allergens and sensitizers. Human skin is always exposed to toxic particles; therefore, in the present study we addressed the question of whether polyvinylpyrrolidone-coated PtNPs may have any negative effects on skin cells, including predominantly epidermal keratinocytes. In this study, PtNPs of two sizes were used: 5.8 nm and 57 nm, in concentrations of 6.25, 12.5, and 25 µg/mL. Both types of NPs were protected with polyvinylpyrrolidone. Primary keratinocytes were treated for 24 and 48 hours, then cytotoxicity, genotoxicity, morphology, metabolic activity, and changes in the activation of signaling pathways were investigated in PtNP-treated cells. We found that PtNPs trigger toxic effects on primary ker

Published
2013

20. Chemerin Is an Antimicrobial Agent in Human Epidermis

Author

Banas, Magdalena, Zabieglo, Katarzyna, Kasetty, Gopinath, Kapinska-Mrowiecka, Monika, Borowczyk, Julia, Drukala, Justyna, Murzyn, Krzysztof, Zabel, Brian A., Butcher, Eugene C., Schroeder, Jens M., Schmidtchen, Artur, Cichy, Joanna, Banas, Magdalena, Zabieglo, Katarzyna, Kasetty, Gopinath, Kapinska-Mrowiecka, Monika, Borowczyk, Julia, Drukala, Justyna, Murzyn, Krzysztof, Zabel, Brian A., Butcher, Eugene C., Schroeder, Jens M., Schmidtchen, Artur, and Cichy, Joanna

Abstract

Chemerin, a chemoattractant ligand for chemokine-like receptor 1 (CMKLR1) is predicted to share similar tertiary structure with antibacterial cathelicidins. Recombinant chemerin has antimicrobial activity. Here we show that endogenous chemerin is abundant in human epidermis, and that inhibition of bacteria growth by exudates from organ cultures of primary human skin keratinocytes is largely chemerin-dependent. Using a panel of overlapping chemerin-derived synthetic peptides, we demonstrate that the antibacterial activity of chemerin is primarily mediated by Val 66 -Pro 85, which causes direct bacterial lysis. Therefore, chemerin is an antimicrobial agent in human skin.

Published
2013

21. Correction: Chemerin Is an Antimicrobial Agent in Human Epidermis

Author

Banas, Magdalena, primary, Zabieglo, Katarzyna, additional, Kasetty, Gopinath, additional, Kapinska-Mrowiecka, Monika, additional, Borowczyk, Julia, additional, Drukala, Justyna, additional, Murzyn, Krzysztof, additional, Zabel, Brian A., additional, Butcher, Eugene C., additional, Schroeder, Jens M., additional, Schmidtchen, Artur, additional, and Cichy, Joanna, additional

Published
2013
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22. Chemerin is an antimicrobial agent in human epidermis. (P1257)

Author

Cichy, Joanna, primary, Banas, Magdalena, additional, Zabieglo, Katarzyna, additional, Kasetty, Gopinath, additional, Kapinska-Mrowiecka, Monika, additional, Borowczyk, Julia, additional, Drukala, Justyna, additional, Murzyn, Krzysztof, additional, Zabel, Brian, additional, Butcher, Eugene, additional, Schroeder, Jens, additional, and Schmidtchen, Arthur, additional

Published
2013
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23. Chemerin Is an Antimicrobial Agent in Human Epidermis

Author

Banas, Magdalena, primary, Zabieglo, Katarzyna, additional, Kasetty, Gopinath, additional, Kapinska-Mrowiecka, Monika, additional, Borowczyk, Julia, additional, Drukala, Justyna, additional, Murzyn, Krzysztof, additional, Zabel, Brian A., additional, Butcher, Eugene C., additional, Schroeder, Jens M., additional, Schmidtchen, Artur, additional, and Cichy, Joanna, additional

Published
2013
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24. Effect of silver nanoparticles on human primary keratinocytes

Author

Szmyd, Radoslaw, primary, Goralczyk, Anna Grazyna, additional, Skalniak, Lukasz, additional, Cierniak, Agnieszka, additional, Lipert, Barbara, additional, Filon, Francesca Larese, additional, Crosera, Matteo, additional, Borowczyk, Julia, additional, Laczna, Eliza, additional, Drukala, Justyna, additional, Klein, Andrzej, additional, and Jura, Jolanta, additional

Published
2012
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25. Fenofibrate enhances barrier function of endothelial continuum within the metastatic niche of prostate cancer cells

Author

Piwowarczyk, Katarzyna, Wybieralska, Ewa, Baran, Jarosaw, Borowczyk, Julia, Rybak, Paulina, Kosiska, Milena, Wodarczyk, Anna Julia, Michalik, Marta, Siedlar, Maciej, Madeja, Zbigniew, Dobrucki, Jerzy, Reiss, Krzysztof, and Czy, Jarosaw

Abstract

Objective:Extravasation of circulating cancer cells is an important step of the metastatic cascade and a potential target for anti-cancer strategies based on vasoprotective drugs. Reports on anti-cancer effects of fenofibrate (FF) prompted us to analyze its influence on the endothelial barrier function during prostate cancer cell diapedesis.Research design and methods:In vitroco-cultures of endothelial cells with cancer cells imitate the ‘metastatic niche’ in vivo.We qualitatively and quantitatively estimated the effect of 25 μM FF on the events which accompany prostate carcinoma cell diapedesis, with the special emphasis on endothelial cell mobilization.Results:Fenofibrate attenuated cancer cell diapedesis via augmenting endothelial cell adhesion to the substratum rather than through the effect on intercellular communication networks within the metastatic niche. The inhibition of endothelial cell motility was accompanied by the activation of PPARα-dependent and PPARα-independent reactive oxygen species signaling, Akt and focal adhesion kinase (FAK) phosphorylation, in the absence of cytotoxic effects in endothelial cells.Conclusions:Fenofibrate reduces endothelial cell susceptibility to the paracrine signals received from prostate carcinoma cells, thus inhibiting endothelial cell mobilization and reducing paracellular permeability of endothelium in the metastatic niche. Our data provide a mechanistic rationale for extending the clinical use of FF and for the combination of this well tolerated vasoactive drug with the existing multidrug regimens used in prostate cancer therapy.

Published
2015
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26. Effects triggered by platinum nanoparticles on primary keratinocytes.

Author

Konieczny, Piotr, Goralczyk, Anna Grazyna, Szmyd, Radoslaw, Skalniak, Lukasz, Koziel, Joanna, Filon, Francesca Larese, Crosera, Matteo, Cierniak, Agnieszka, Zuba-Surma, Ewa K., Borowczyk, Julia, Laczna, Eliza, Drukala, Justyna, Pyza, Elzbieta, Semik, Danuta, Woznicka, Olga, Klein, Andrzej, and Jura, Jolanta

Published
2013
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27. A methylome and transcriptome analysis of normal human scar cells reveals a role for FOXF2in scar maintenance

Author

Stevenson, Andrew W., Melton, Phillip E., Moses, Eric.K., Wallace, Hilary J., Wood, Fiona M., Rea, Suzanne, Danielsen, Patricia L., Alghamdi, Mansour, Hortin, Nicole, Borowczyk, Julia, Deng, Zhenjun, Manzur, Mitali, and Fear, Mark W.

Abstract

Scar is maintained for life and increases in size during periods of growth such as puberty. Epigenetic changes in fibroblasts after injury may underpin the maintenance and growth of scar. Here, we, combined methylome and transcriptome data from normotrophic mature scar and contralateral uninjured normal skin fibroblasts to identify potential regulators of scar maintenance. 219 significantly differentially expressed and 1199 significantly differentially methylated promoters were identified, of which there were 12 genes both significantly differentially methylated and expressed. Of these the two transcription factors, Forkhead Box F2 (FOXF2) and Mohawk Homeobox (MKX) were selected for further analysis. Immunocytochemistry and qPCR suggested FOXF2 but not MKX had elevated expression in scar fibroblasts. Using RNASeq, FOXF2knockdown was shown to significantly reduce expression of extracellular matrix related genes, whilst MKXdid not appear to affect similar pathways. Finally, FOXF2knockdown was also shown to significantly decrease collagen I production in scar and keloid fibroblasts. This study provides insights into the maintenance of normotrophic scar, suggesting FOXF2is an important regulator of this process. Targeting genes responsible for maintenance of scar phenotype may ameliorate scar appearance and improve patient outcomes in the future.

Published
2021
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28. [Metabolic changes in bone marrow in patients submitted to immunosuppressive treatment].

Author

Sporniak-Tutak K, Borowczyk J, Domański L, Myśliwiec L, and Drukała J

Subjects
Bone Marrow drug effects, Bone Marrow Cells cytology, Bone Marrow Cells drug effects, Bone Marrow Cells metabolism, Humans, Mesenchymal Stem Cells metabolism, Transplants, Bone Marrow metabolism, Immunocompromised Host physiology, Immunosuppressive Agents pharmacology, Organ Transplantation
Abstract

A historical outline of the immunosuppressive treatment and related developments in transplantology is presented here. It is accompanied by a description of the essential knowledge of the bone marrow histology as well as of the role of mesenchymal stem cells (MSCs) in the regenerative processes occurring in the organism following tissue damage. We have reviewed contemporary knowledge of negative (quantitative and qualitative) changes in bone tissues caused by immunosuppressive treatment after an organ transplantation and mentioned prophylactic measures which may be used in such cases. We also describe basic immunosuppressive medications taking into account their side effects on the organism of the patient and, in particular, on the bone marrow metabolism as well as the mechanisms of their action at the cellular level.

Published
2010

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