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8 results on '"Borrelia burgdorferi s. l"'

1. A case of combined infection with tick-borne encephalitis and lyme borreliosis with severe meningoencephalitis and complete recovery

Author

Yekaterina O Ostapchuk, Andrey M Dmitrovskiy, Elena A Pak, and Yuliya V Perfilyeva

Subjects
borrelia burgdorferi s. l, kazakhstan, lyme neuroborreliosis, meningoencephalitis, tick-borne encephalitis, tick-borne encephalitis virus, Infectious and parasitic diseases, RC109-216
Abstract

Here, we present a case of severe meningoencephalitis caused by combined infection with tick-borne encephalitis (TBE) and Lyme borreliosis (LB) in a 25-year-old woman in a rural area of Zhambyl region, Kazakhstan. She presented with fever, nausea, vomiting, weakness, sweating, severe headache, arthralgia, and malaise. The course of illness was further complicated by encephalitis with symmetric lesions of the midbrain cerebral peduncles and serous meningitis. TBE and LB co-infection were established by a two-fold increase in serum IgG titers between day 21 and day 25 of illness. Both infections responded well to combined therapy with human TBE immunoglobulins, antibiotics, antiviral drugs, glucocorticoids, and diuretics. The outcome of the disease was favorable and the patient recovered completely.

Published
2023
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2. A Case of Combined Infection with Tick-Borne Encephalitis and Lyme Borreliosis with Severe Meningoencephalitis and Complete Recovery.

Author

Ostapchuk, Yekaterina O., Dmitrovskiy, Andrey M., Pak, Elena A., and Perfilyeva, Yuliya V.

Subjects
TICK-borne encephalitis, MENINGOENCEPHALITIS, INTRACRANIAL hypertension, INFECTION, RURAL women, LYME disease
Abstract

Here, we present a case of severe meningoencephalitis caused by combined infection with tick-borne encephalitis (TBE) and Lyme borreliosis (LB) in a 25-year-old woman in a rural area of Zhambyl region, Kazakhstan. She presented with fever, nausea, vomiting, weakness, sweating, severe headache, arthralgia, and malaise. The course of illness was further complicated by encephalitis with symmetric lesions of the midbrain cerebral peduncles and serous meningitis. TBE and LB co-infection were established by a two-fold increase in serum IgG titers between day 21 and day 25 of illness. Both infections responded well to combined therapy with human TBE immunoglobulins, antibiotics, antiviral drugs, glucocorticoids, and diuretics. The outcome of the disease was favorable and the patient recovered completely. [ABSTRACT FROM AUTHOR]

Published
2023
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3. Prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum in Ixodid Ticks from Southeastern Ukraine.

Author

Kovryha, Nadia, Tsyhankova, Ala, Zelenuchina, Olena, Mashchak, Olexandr, Terekhov, Roman, and Rogovskyy, Artem S.

Subjects
*ANAPLASMA phagocytophilum, *CASTOR bean tick, *BORRELIA burgdorferi, *TICKS, *LYME disease, *TICK-borne diseases, *DERMACENTOR
Abstract

Objectives: Tick-borne diseases have emerged as an increasing medical problem in the world. Being the most prevalent ixodid ticks in Europe, Ixodes ricinus and Dermacentor reticulatus are responsible for transmission of numerous zoonotic pathogens (e.g., human granulocytic anaplasmosis and Lyme borreliosis). Despite their public health significance, studies on the prevalence of tick-borne agents are scare for Eastern Europe. The objective of this study was to examine the prevalence of Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Borrelia burgdorferi sensu lato (B. burgdorferi s. l.) in ixodid ticks from Southeastern Ukraine. Methods: Over a 5-year period (2014–2018), 358 questing and 389 engorged ixodid ticks were collected from Southeastern Ukraine (Zaporizhzhya region). The ticks were identified as Dermacentor marginatus, D. reticulatus, I. ricinus, and Rhipicephalus rossicus. Nucleic acid samples extracted from tick pools were subjected to RT-PCR analyses for A. phagocytophilum, E. chaffeensis, and B. burgdorferi s. l. Results: The examined ixodid ticks tested negative for the aforementioned pathogens with the exception of I. ricinus ticks. For questing I. ricinus ticks, minimum infection rates of A. phagocytophilum and B. burgdorferi s. l. were, respectively, 4.2–7.7% and 8.6–12.7%. Conclusions: These findings will be valuable for medical and veterinary practitioners when risks associated with tick-borne diseases are assessed for southeastern regions of Ukraine. [ABSTRACT FROM AUTHOR]

Published
2021
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4. Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic

Author

Ondřej Bonczek, Alena Žákovská, Lýdia Vargová, and Omar Šerý

Subjects
Borrelia burgdorferi s. l, Borrelia spielmanii, Ixodes ricinus, genotyping, RealTime PCR, DNA sequencing, Agriculture, Environmental sciences, GE1-350
Abstract

Introduction During 2008–2012, a total of 466 ticks Ixodes ricinus removed from humans were collected and tested for the presence of Borrelia burgdorferi sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ). Objective The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. Material and Methods DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene Borrelia LC (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database. Results A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as Borrelia spielmanii (7.1%). Other representatives of Bbsl were also observed: B. afzelii (70.0%), B. garinii (10.0%), B. valaisiana (8.6%), and B. burgdorferi s. s. (4.3%). Conclusions A general view of the spreading of Bbsl in the South Moravian region was demonstrated. The most interesting result of the study is the finding of B. spielmanii for the first time in this region.

Published
2015
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5. Prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum in Ixodid Ticks from Southeastern Ukraine

Author

Olexandr Mashchak, Olena Zelenuchina, Nadia Kovryha, Artem S. Rogovskyy, Ala Tsyhankova, and Roman Terekhov

Subjects
0301 basic medicine, Ixodes ricinus, animal diseases, 030231 tropical medicine, Biology, Microbiology, ticks, 03 medical and health sciences, Borrelia burgdorferi s. l, 0302 clinical medicine, Dermacentor reticulatus, Borrelia burgdorferi Group, Virology, parasitic diseases, Prevalence, Ehrlichia chaffeensis, Animals, Borrelia burgdorferi, Rickettsia, Lyme borreliosis, Dermacentor, Ixodes, Borrelia, fungi, Original Articles, 030108 mycology & parasitology, biology.organism_classification, bacterial infections and mycoses, Anaplasma phagocytophilum, Infectious Diseases, Ukraine
Abstract

Objectives: Tick-borne diseases have emerged as an increasing medical problem in the world. Being the most prevalent ixodid ticks in Europe, Ixodes ricinus and Dermacentor reticulatus are responsible for transmission of numerous zoonotic pathogens (e.g., human granulocytic anaplasmosis and Lyme borreliosis). Despite their public health significance, studies on the prevalence of tick-borne agents are scare for Eastern Europe. The objective of this study was to examine the prevalence of Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Borrelia burgdorferi sensu lato (B. burgdorferi s. l.) in ixodid ticks from Southeastern Ukraine. Methods: Over a 5-year period (2014–2018), 358 questing and 389 engorged ixodid ticks were collected from Southeastern Ukraine (Zaporizhzhya region). The ticks were identified as Dermacentor marginatus, D. reticulatus, I. ricinus, and Rhipicephalus rossicus. Nucleic acid samples extracted from tick pools were subjected to RT-PCR analyses for A. phagocytophilum, E. chaffeensis, and B. burgdorferi s. l. Results: The examined ixodid ticks tested negative for the aforementioned pathogens with the exception of I. ricinus ticks. For questing I. ricinus ticks, minimum infection rates of A. phagocytophilum and B. burgdorferi s. l. were, respectively, 4.2–7.7% and 8.6–12.7%. Conclusions: These findings will be valuable for medical and veterinary practitioners when risks associated with tick-borne diseases are assessed for southeastern regions of Ukraine.

Published
2021

6. Laimo boreliozės paplitimo Lietuvoje ir ligos sukėlėjo Borrelia burgdorferi s. l. ospA geno sekų analizė

Author

Juodišiūtė, Indrė, Radzijevskaja, Jana, Galdikaitė, Eglė, and Vytautas Magnus University

Subjects
Borrelia burgdorferi s. l, Laimo boreliozė, OspA genas, Paplitimas, OspA gene, Prevalence, Biology, Lyme borreliosis
Abstract

Laimo boreliozė (LB) yra labiausiai paplitusi erkių platinama liga. Ligos sukėlėjus perneša Ixodes rūšies erkės. Didelį borelijų paplitimą lemia platus rezervuarinių šeimininkų ratas. Šį susirgimą gali sukelti kelios B. burgdorferi s. l. komplekso bakterijų rūšys: B. afzelii, B. garinii, B. burgdorferi sensu stricto. Šiame darbe buvo tirtos 134 Ixodes ricinus erkės nuo 8 elninių gyvūnų (4 stirnų ir 4 elnių) ir 73 erkės nuo žolės (elninių teritorijoje). Naudojant dauginės polimerazės grandininės reakcijos (PGR) medodą, 9 - iose (4,3%) iš jų buvo nustatytas užsikrėtimas Borrelia burgdorferi s. l. patogenais, septyniuose mėginiuose buvo identifikuota B. afzelii, viename - B. garinii rūšis. Viename mėginyje buvo nustatyta Borrelia spp., kuri nebuvo identifikuota. Atlikta B. burgdorferi s. l. sekų analizė pagal ospA geną, naudojantis genų banko duomenimis, atskleidė polimorfizmo lygį tarp trijų, žmogui patogeniškų Borrelia rūšių ir parodė padermių pasiskirstymą skirtingose šalyse bei šeimininkuose. Variabiliausios nustatytos B. garinii ospA geno sekos, rasta 361 variabili vieta (nukleotidų įvairovė π=0,072), aptikta 18 sekų variantų. Mažesnis variabilumas nustatytas B. burgdorferi s. s. sekose – aptiktos 266 variabilios vietos (nukleotidų įvairovė π=0,16) ir 12 sekų variantų bei B. afzelii sekose – aptiktos 257 variabilios vietos (nukleotidų įvairovė π=0,17) ir 4 sekų variantai. Didesni skirtumai buvo identifikuoti tarp B. afzelii ospA geno sekų (vidutinis genetinis atstumas... [toliau žr. visą tekstą] Lyme borreliosis (LB) is the most common tick - born disease. The pathogens are transmitted by infected ticks belonging to a species of the genus Ixodes. The high prevalence of Borrelia is determined by a wide circle of reservoir hosts. The disease can be caused by a few bacterial species of B. burgdorferi s.l. complex, such as: B. afzelii, B. garinii and B. burgdorferi sensu stricto. 134 Ixodes ricinus ticks have been explored in the thesis, collected from 8 certine animals (4 roe deer and 4 deer) and 73 ticks collected from grass (in the territory of certine animals). Using multiplex polymerase chain reaction (PCR) method, the infection with Borrelia burgdorferi s. l. pathogens was identified in 9 (4.3%), B. afzelii was identified in seven samples, while B. garinii species was found in one of them. Borrelia spp. was found in one sample, which has not been identified. A sequence analysis B. burgdorferi s. l. implemented according to the ospA gene by using a gene bank data revealed level of polymorphism among three Borrelia species pathogenic to human and showed the distribution of strains in the different countries and hosts. The most variable gene sequences are B. garinii ospA. It is found 361 variable place (nucleotide diversity π=0,072), ant detected 18 sequence variants. Less variability found in B. burgdorferi s. s. sequences: 266 variable places are found and 12 sequence variants (nucleotide diversity π=0,16) and 257 variable places are discovered in B. afzelii... [to full text]

Published
2014

7. Laimo boreliozės paplitimo Lietuvoje ir ligos sukėlėjo Borrelia burgdorferi s. l. ospA geno sekų analizė

Author

Radzijevskaja, Jana, Galdikaitė, Eglė, Vytautas Magnus University, Juodišiūtė, Indrė, Radzijevskaja, Jana, Galdikaitė, Eglė, Vytautas Magnus University, and Juodišiūtė, Indrė

Abstract

Laimo boreliozė (LB) yra labiausiai paplitusi erkių platinama liga. Ligos sukėlėjus perneša Ixodes rūšies erkės. Didelį borelijų paplitimą lemia platus rezervuarinių šeimininkų ratas. Šį susirgimą gali sukelti kelios B. burgdorferi s. l. komplekso bakterijų rūšys: B. afzelii, B. garinii, B. burgdorferi sensu stricto. Šiame darbe buvo tirtos 134 Ixodes ricinus erkės nuo 8 elninių gyvūnų (4 stirnų ir 4 elnių) ir 73 erkės nuo žolės (elninių teritorijoje). Naudojant dauginės polimerazės grandininės reakcijos (PGR) medodą, 9 - iose (4,3%) iš jų buvo nustatytas užsikrėtimas Borrelia burgdorferi s. l. patogenais, septyniuose mėginiuose buvo identifikuota B. afzelii, viename - B. garinii rūšis. Viename mėginyje buvo nustatyta Borrelia spp., kuri nebuvo identifikuota. Atlikta B. burgdorferi s. l. sekų analizė pagal ospA geną, naudojantis genų banko duomenimis, atskleidė polimorfizmo lygį tarp trijų, žmogui patogeniškų Borrelia rūšių ir parodė padermių pasiskirstymą skirtingose šalyse bei šeimininkuose. Variabiliausios nustatytos B. garinii ospA geno sekos, rasta 361 variabili vieta (nukleotidų įvairovė π=0,072), aptikta 18 sekų variantų. Mažesnis variabilumas nustatytas B. burgdorferi s. s. sekose – aptiktos 266 variabilios vietos (nukleotidų įvairovė π=0,16) ir 12 sekų variantų bei B. afzelii sekose – aptiktos 257 variabilios vietos (nukleotidų įvairovė π=0,17) ir 4 sekų variantai. Didesni skirtumai buvo identifikuoti tarp B. afzelii ospA geno sekų (vidutinis genetinis atstumas... [toliau žr. visą tekstą], Lyme borreliosis (LB) is the most common tick - born disease. The pathogens are transmitted by infected ticks belonging to a species of the genus Ixodes. The high prevalence of Borrelia is determined by a wide circle of reservoir hosts. The disease can be caused by a few bacterial species of B. burgdorferi s.l. complex, such as: B. afzelii, B. garinii and B. burgdorferi sensu stricto. 134 Ixodes ricinus ticks have been explored in the thesis, collected from 8 certine animals (4 roe deer and 4 deer) and 73 ticks collected from grass (in the territory of certine animals). Using multiplex polymerase chain reaction (PCR) method, the infection with Borrelia burgdorferi s. l. pathogens was identified in 9 (4.3%), B. afzelii was identified in seven samples, while B. garinii species was found in one of them. Borrelia spp. was found in one sample, which has not been identified. A sequence analysis B. burgdorferi s. l. implemented according to the ospA gene by using a gene bank data revealed level of polymorphism among three Borrelia species pathogenic to human and showed the distribution of strains in the different countries and hosts. The most variable gene sequences are B. garinii ospA. It is found 361 variable place (nucleotide diversity π=0,072), ant detected 18 sequence variants. Less variability found in B. burgdorferi s. s. sequences: 266 variable places are found and 12 sequence variants (nucleotide diversity π=0,16) and 257 variable places are discovered in B. afzelii... [to full text]

Published
2014

8. Molekulinių žymenų panaudojimas Laimo ligos sukėlėjų Borrelia burgdorferi s. l. bakterijų identifikavimui

Author

Radzijevskaja, Jana, Paulauskas, Algimantas, Tamutė, Brigita, Vytautas Magnus University, Namavičiūtė, Eglė, Radzijevskaja, Jana, Paulauskas, Algimantas, Tamutė, Brigita, Vytautas Magnus University, and Namavičiūtė, Eglė

Abstract

Šio darbo tyrimo objektas - Borrelia burgdorferi sensu lato bakterijos, žmogaus organizme sukeliančios Laimo boreliozę. Jų nustatymas iki šiol problematiškas, nėra vieno patikimo metodo nustatyti šią ligą. Todėl siekiant išsiaiškinti kelių metodų tinkamumą, buvo suformuluotas darbo tikslas – panaudojant skirtingus molekulinius žymenis ir metodus identifikuoti B. burgdorferi s. l. bakterijų genotipus skirtinguose organizmuose. Tyrime naudota medžiaga, surinkta 2010 metais pietvakarių Norvegijoje (580 2' 3" N, 70 28' 43" E). Molekuliniams tyrimams naudotos 336 Ixodes ricinus lervos nuo graužikų, 169 nimfos nuo augalijos ir 18 graužikų DNR. DNR išskyrimui naudoti trys skirtingi metodai, parinkti priklausomai nuo patogenų identifikavimui pasirinktų metodų. Tyrimų metu panaudoti penki molekuliniai žymenys (16S rRNR, ospA, hbb, rpoB genai ir 16S (rrsA) – 23S (rrlA) tarpgeninis regionas) bei penki skirtingi metodai (standartinė, dauginė, lizdinė, tikro laiko PGR bei sekoskaita). Erkės nuo graužikų pirmiausia buvo patikrintos tikro laiko PGR metodu pagal 16S rRNR geną. Nustatytas 11 % (36 iš 336) užkrėstumas B. burgdorferi s. l. patogenais. Siekiant palyginti kito metodo panaudojimo galimybes, visos teigiamos erkės pagal 16S rRNR geną buvo patikrintos standartinės PGR metodu pagal ospA geną. Pasitvirtino dešimtadalis infekuotų erkių (11 % – 4 iš 36). Erkių nuo augalijos užkrėstumas tikrinant standartinės PGR metodu pagal ospA geną – 9 % (15 iš 169), graužikų – 40 % (7 iš 18). Tikro... [toliau žr. visą tekstą], The object of this study was Borrelia burgdorferi sensu lato bacteria, which cause Lyme borreliosis in human organism. The detection of B. burgdorferi s. l. is still complicated, there is not only reliable method to diagnose the disease. To elucidate the relevance of a few methods, the purpose was formed: to detect and identify B. burgdorferi s. l. genotypes in several organisms using different molecular markers and methods. The material of the study was collected during summer and autumn of 2010 in southern Norway (580 2' 3" N, 70 28' 43" E). 336 Ixodes ricinus larvae collected from rodents, 169 nymphs collected from vegetation and 18 DNA of rodents were used for molecular survey. Three different methods of DNA extraction, five different molecular markers (16S rRNR, ospA, hbb, rpoB genes and 16S (rrsA) – 23S (rrlA) intergenic spacer) and five different methods (standard, multiplex, nested, real time PCR and sequencing) were used in this study. Ticks collected from vegetation at first were screened by real time PCR based on 16S rRNR gene. Infection rate of B. burgdorferi s. l. pathogens in ticks collected from vegetation was 11 % (36 from 336). To compare a relevance of other method, all the positive ticks after screening were tested by standard PCR based on ospA gene. It was proven a tenth of infected ticks (11 %: 4 from 36). Infection rate by the same method in ticks from vegetation was 9 % (15 from 169), in rodents 40 % (7 from 18). The real time PCR based on hbb gene... [to full text]

Published
2011

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