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Bottle gourd (Lagenaria siceraria (Molina) Standl.) is an important crop in Jordan, but it remains under-researched. The primary aim of this study was to evaluate the genetic variability existing among different bottle gourd landraces in Jordan using Agro-morphological traits and molecular markers. Bottle gourd landraces were collected from various locations in Jordan; phenotypic differences and genetic variation using ISSR were studied between collected landraces. Results show that phenotypic coefficients of variance were larger than their genotypic coefficients of variance for all characters, demonstrating that the environment had an impact on these features. High genetic advance was found for plant length, number of leaves, leaf width and seed area; this could be explained by additive gene action. High heritability >60% estimates were observed. Principal component analysis identified two principal components responsible for 69.6% of total variation. Plant height showed positive association with number of tendrils, number of leaves and leaf length. 246 amplified markers were obtained using 24 ISSR primers, 135 of which were polymorphic. Genetic distance varied between 0.78 to 0.92 based on molecular analysis and ranged from 0.42 to 0.91 for agro-morphological data. Dendrograms constructed based on morphological and ISSR data clustered landraces to six and five main groups, respectively. The results of this work could be used in future bottle gourd breeding programs. [ABSTRACT FROM AUTHOR]

The bottle gourd [Lagenaria siceraria (Molina) Standl.] is often utilized as a rootstock for watermelon grafting. This practice effectively mitigates the challenges associated with continuous cropping obstacles in watermelon cultivation. The lower ground temperature has a direct impact on the rootstocks' root development and nutrient absorption, ultimately leading to slower growth and even the onset of yellowing. However, the mechanisms underlying the bottle gourd's regulation of root growth in response to low root zone temperature (LRT) remain elusive. Understanding the dynamic response of bottle gourd roots to LRT stress is crucial for advancing research regarding its tolerance to low temperatures. In this study, we compared the physiological traits of bottle gourd roots under control and LRT treatments; root sample transcriptomic profiles were monitored after 0 h, 48 h and 72 h of LRT treatment. LRT stress increased the malondialdehyde (MDA) content, relative electrolyte permeability and reactive oxygen species (ROS) levels, especially H2O2 and O2−. Concurrently, LRT treatment enhanced the activities of antioxidant enzymes like superoxide dismutase (SOD) and peroxidase (POD). RNA-Seq analysis revealed the presence of 2507 and 1326 differentially expressed genes (DEGs) after 48 h and 72 h of LRT treatment, respectively. Notably, 174 and 271 transcription factors (TFs) were identified as DEGs compared to the 0 h control. We utilized quantitative real-time polymerase chain reaction (qRT-PCR) to confirm the expression patterns of DEGs belonging to the WRKY, NAC, bHLH, AP2/ERF and MYB families. Collectively, our study provides a robust foundation for the functional characterization of LRT-responsive TFs in bottle gourd roots. Furthermore, these insights may contribute to the enhancement in cold tolerance in bottle gourd-type rootstocks, thereby advancing molecular breeding efforts. [ABSTRACT FROM AUTHOR]

Fruit size is a crucial agronomic trait in bottle gourd, impacting both yield and utility. Despite its significance, the regulatory mechanism governing fruit size in bottle gourd remains largely unknown. In this study, we used bottle gourd (small-fruited H28 and large-fruited H17) parent plants to measure the width and length of fruits at various developmental stages, revealing a single 'S' growth curve for fruit expansion. Paraffin section observations indicated that both cell number and size significantly influence bottle gourd fruit size. Through bulked segregant analysis and combined genotype–phenotype analysis, the candidate interval regulating fruit size was pinpointed to 17,747,353 bp–18,185,825 bp on chromosome 9, encompassing 0.44 Mb and including 44 genes. Parental fruits in the rapid expansion stage were subjected to RNA-seq, highlighting that differentially expressed genes were mainly enriched in pathways related to cell wall biosynthesis, sugar metabolism, and hormone signaling. Transcriptome and resequencing analysis, combined with gene function annotation, identified six genes within the localized region as potential regulators of fruit size. This study not only maps the candidate interval of genes influencing fruit size in bottle gourd through forward genetics, but also offers new insights into the potential molecular mechanisms underlying this trait through transcriptome analysis. [ABSTRACT FROM AUTHOR]

Purpose: This research endeavor aimed to produce composts using banana plant leftovers (stem and leaves) and animal manures (poultry and cattle), and subsequently assess their nutrient concentrations and the resulting impact on bottle gourd growth. Method: Two distinct composts were prepared by utilizing banana plants (stems + leaves) blended with cattle and poultry manure in cemented pits. Afterwards the prepared composts were used as soil amendments. The treatments included T1: control, T2: Recommended NPK, T3: 10-ton compost 1 Banana leaves + Poultry manure T4: 10-ton compost 2 Banana leaves + Cattle manure, T5: 5-ton compost 1 + ½ NPK, and T6: 5 ton compost 2 + ½ NPK. Results: The study revealed that composts significantly improved plant development and yield, with enhanced effectiveness when combined with NPK fertilizers. The treatments led to substantial increases in vine length (25-61%), branch number (25-72%), fruit dimensions, weight (14-79%), and yield (13-55%), with NPK treatment showing the highest gains. The N concentration doubled, while P and K concentrations increased 13-fold and 5-fold, respectively. Both sole and integrated NPK + Compost 2 applications effectively enhanced N concentration, while the highest P and K increases were observed with the sole NPK application, closely followed by the integrated approach. Conclusion: Composting effectively recycled banana plant leftovers, cattle, and poultry manure, producing high-quality compost that significantly enhances growth, yield, and nutrient accumulation in bottle gourd. The combined use of compost and NPK fertilizers outperforms their individual application, suggesting that this integrated nutrient management strategy is ideal for optimizing bottle gourd production in low-fertility soils. Research Highlights • Composting banana, cattle, and poultry waste produced high-quality compost, supporting sustainable agriculture • The physico-chemical characteristics of compost were highly influenced by the materials (feedstocks) used during the composting process • Compost application boosted growth, yield, and nutrient uptake in bottle gourd • Integrated compost and NPK application enhanced productivity and soil health

Abstract The squash family (Cucurbitaceae) contains some of the most important crops cultivated worldwide and has played an important ecological, economic, and cultural role for millennia. In the American tropics, squashes were among the first cultivated crop species, but little is known about how their domestication unfolded. Here, we employ direct radiocarbon dating and morphological analyses of desiccated cucurbit seeds, rinds, and stems from El Gigante Rockshelter in Honduras to reconstruct human practices of selection and cultivation of Lagenaria siceraria, Cucurbita pepo, and Cucurbita moschata. Direct radiocarbon dating indicates that humans started using Lagenaria and wild Cucurbita starting ~ 10,950 calendar years before present (cal B.P.), primarily as watertight vessels and possibly as cooking and drinking containers. A rind directly dated to 11,150–10,765 cal B.P. represents the oldest known bottle gourd in the Americas. Domesticated C. moschata subsequently appeared ~ 4035 cal B.P., followed by domesticated C. pepo ~ 2190 cal B.P. associated with increasing evidence for their use as food crops. Multivariate statistical analysis of seed size and shape show that the archaeological C. pepo assemblage exhibits significant variability, representing at least three varieties: one similar to present-day zucchini, another like present-day vegetable marrow, and a native cultivar without modern analogs. Our archaeobotanical data supports the hypothesis that Indigenous cucurbit use started in the Early Holocene, and that agricultural complexity during the Late Holocene involved selective breeding that encouraged crop diversification.

The present investigation was carried out at Horticultural Research Farm, Department of Horticulture, North Eastern Hill University, Tura Campus, Chasingre, Meghalaya, India during 2019 in a Randomized Block Design with three replications. Genetic divergence was assessed among thirty indegenous genotypes of bottle gourd for thirty-three quantitative characters using Mahalanobis' D2 statistics. The genotypes were grouped into seven clusters. Maximum number of genotypes including seven genotypes was grouped in cluster IV (GHA-30, GHA-8, GHA-21, GHA-20, GHA-23, GHA-29 and GHA-25). Maximum inter-cluster distance was found between cluster I and cluster III (10.940) and minimum inter-cluster distance was recorded between cluster VII and cluster VI (7.425). Cluster IV showed highest cluster mean for maximum characters namely, number of primary branches (30.83), number of marketable fruit harvest (4.60), fruit width (15.24), fruit weight (3005.65), number of fruits per plant (17.92), total carbohydrate % (2.73), calcium mg/100g (11.95), yield of marketable fruits kg/plant (54.20) and yield t/ha (135.50). Cluster VII exhibited highest mean for five characters namely, vine length (461.93), days to last fruit harvest (158.77), number of leaves (217.13), seed length-breadth ratio (2.41) and ascorbic acid (9.55). Cluster I and Cluster II exhibited highest mean for five characters, sex ratio (0.74), number of seeds per fruit (449.65), 100 seed weight (13.43), total soluble protein % (0.95) and total phenols % (44.04). In the present study it was found that Cluster I (GHA-4, GHA-2, GHA-5 and GHA-1) was highly divergent from all other genotypes. [ABSTRACT FROM AUTHOR]

Summary: Bottle gourd (Lagenaria siceraria (Mol.) Strandl.) is an economically important vegetable crop and one of the earliest domesticated crops. However, the population history and genomic diversification of bottle gourd have not been extensively studied.We generated a comprehensive bottle gourd genome variation map from genome sequences of 197 world‐wide representative accessions, which enables a genome‐wide association study for identifying genomic loci associated with resistance to zucchini yellow mosaic virus, and constructed a bottle gourd pangenome that harbors 1534 protein‐coding genes absent in the reference genome.Demographic analyses uncover that domesticated bottle gourd originated in Southern Africa c. 12 000 yr ago, and subsequently radiated to the New World via the Atlantic drift and to Eurasia through the efforts of early farmers in the initial Holocene. The identified highly differentiated genomic regions among different bottle gourd populations harbor many genes contributing to their local adaptations such as those related to disease resistance and stress tolerance. Presence/absence variation analysis of genes in the pangenome reveals numerous genes including those involved in abiotic/biotic stress responses that have been under selection during the world‐wide expansion of bottle gourds.The bottle gourd variation map and pangenome provide valuable resources for future functional studies and genomics‐assisted breeding. [ABSTRACT FROM AUTHOR]

Drying food is an effective method to preserve fruits and vegetables, increasing their shelf life and preventing germination. Bottle gourds, known for their high nutritional value, can rot quickly due to their high moisture content, causing biochemical and microbial changes. To improve shelf life, moisture can be removed to prevent microbial activity. Different drying methods are used to preserve gourd family vegetables for long periods and off-season. Indirect solar drying shields foods from UV rays, dust, insects, and other contamination by avoiding direct exposure. This study aimed to utilize an indirect solar dryer to research the potential for drying bottle gourds and conduct physicochemical analyses for the dried products to compare the drying techniques. In this experiment, different samples of bottle gourd slices were dried using an indirect solar dryer and compared with sun and shade drying. Under physicochemical properties, moisture content (MC), retention of color, thickness, and shrinkage of dried samples were analyzed and compared. The result showed that the time taken to reach 10% moisture was 13 h for samples dried with sun drying. While shade and indirect solar drying took 17 h. The Midilli-Kucuk model outperforms other approaches for drying bottle gourd, with the greatest R2 values (0.9967, 0.9950, 0.9936) and the lowest RMSE values (0.0182, 0.0220, 0.0277) for direct sun, shade, and indirect solar drying, respectively. The indirect sun dryer showed good color retention compared to the fresh bottle gourd samples with L* value of 44.08, b* value of 15.40 and a* value of 7.01. The volumetric shrinkage was 97.64% in the direct sun drying method after 15 h, which is higher compared to sun and shade dryers due to the effect of time and temperature. In addition, there was a significant difference (p-value <0.05) between the thickness of the three drying methods. The use of indirect solar drying methods can be highly recommended to get high-quality dried food with zero energy cost. For future studies it is recommended to enhance the function and design of the indirect solar dryer to speed up drying times and improve the quality of dried samples. [ABSTRACT FROM AUTHOR]

The squash family (Cucurbitaceae) contains some of the most important crops cultivated worldwide and has played an important ecological, economic, and cultural role for millennia. In the American tropics, squashes were among the first cultivated crop species, but little is known about how their domestication unfolded. Here, we employ direct radiocarbon dating and morphological analyses of desiccated cucurbit seeds, rinds, and stems from El Gigante Rockshelter in Honduras to reconstruct human practices of selection and cultivation of Lagenaria siceraria, Cucurbita pepo, and Cucurbita moschata. Direct radiocarbon dating indicates that humans started using Lagenaria and wild Cucurbita starting ~ 10,950 calendar years before present (cal B.P.), primarily as watertight vessels and possibly as cooking and drinking containers. A rind directly dated to 11,150–10,765 cal B.P. represents the oldest known bottle gourd in the Americas. Domesticated C. moschata subsequently appeared ~ 4035 cal B.P., followed by domesticated C. pepo ~ 2190 cal B.P. associated with increasing evidence for their use as food crops. Multivariate statistical analysis of seed size and shape show that the archaeological C. pepo assemblage exhibits significant variability, representing at least three varieties: one similar to present-day zucchini, another like present-day vegetable marrow, and a native cultivar without modern analogs. Our archaeobotanical data supports the hypothesis that Indigenous cucurbit use started in the Early Holocene, and that agricultural complexity during the Late Holocene involved selective breeding that encouraged crop diversification. [ABSTRACT FROM AUTHOR]

Abstract Bottle gourd is a good source of health-beneficial bioactive substances. This research aimed at evaluating the effect of different cooking methods (pressure cooking, microwaving, and frying) and extraction solvents (methanol, ethanol and butanol) on bottle gourd fruit phenolic compounds and antioxidant capacity. The quantitative estimation of polyphenolic compounds, flavonoids and tannins was estimated by spectrophotometric methods. The antioxidative properties were evaluated using ferric thiocyanate (FTC), thiobarbituric acid (TBA), ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) tests using the standard methods. Moreover, quantification of individual polyphenols was carried out by high-performance thin layer chromatography (HPTLC) technique. Frying and pressure-cooking thermal treatments were observed to be the best methods for retaining bioactive molecules. When compared to the raw counterpart, the retention level of total phenol content (TPC) in pressure cooked and fried samples was 23.8% and 13.3%, respectively. Similarly, antioxidant capacity in terms of FRAP was observed to increase by 47.26% after pressure cooking and 28.50% after microwave cooking in respect to the raw sample. The HPTLC results showed that this fruit has high antioxidant capacity and is rich in phenolic acid [gallic(17.83 mg g−1dwb), p-coumaric (6.70 mg g−1dwb)and vanillic (60.66 mg g−1dwb] and flavonoids [quercetin (24.64 mg g−1dwb) and myrecetin (20.73 mg g−1dwb].The chromatography indicated destruction and transformation of new phenolic compounds due to heat treatments. The correlation analysis revealed that flavonoids were much more responsible for their antioxidant activity. Cooking methods and extraction solvents affected the retention and recovery of polyphenolic compounds available in bottle gourd fruit. These findings offer valuable information for optimizing cooking techniques to preserve and enhance the nutritional and antioxidant properties of bottle gourd, making it a valuable fruit for a healthy diet. Pressure cooking could be the most suitable cooking treatment as far as retention of bioactive compounds like phenolic acids is concerned. Graphical Abstract

Fruit neck length (FNL) is one of the important commercial characteristics of bottle gourd (Lagenaria siceraria), which is usually desirable by consumers based on their preferences. At present, there has not been extensive research on the genetics of bottle gourd FNL. In this study, an F2 segregating population was constructed using the long fruit neck material H16 and the short fruit neck material H06. Bulked segregant analysis and kompetitive allele-specific PCR techniques were used to identify candidate regions regulating FNL in bottle gourd. InDel markers were used to narrow the final candidate region interval to 0.16 Mb, which contained 8 genes. A combined analysis using a gene annotation library, real-time fluorescence quantitative expression, and sequence analysis of candidate genes in the region indicated that the HG_GLEAN_10011965 gene might be involved in the regulation of bottle gourd FNL and was named LsFNL1.1. Based on the most narrowed candidate gene interval, an InDel marker was developed for molecular marker assisted breeding of bottle gourd FNL. The phenotypic-genotype association rate of this InDel marker was 83%. This study provides a theoretical basis for further exploring the molecular mechanisms underlying bottle gourd fruit traits and can accelerate the breeding of varieties with desirable characteristics. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Heat stress, as a negative factor, severely threatens the quality and production of bottle gourd, which prefers to grow in a warm environment. To understand which genes are involved in the resistance to heat stress in bottle gourd (Lagenaria siceraria (Mol.) Standl.), we analyzed the characteristics of two genetic bottle gourd varieties, "Mei feng"-MF (heat resistant) and "Lv long"-LL (heat sensitive). Under heat stress, MF plants exhibited a higher survival rate, lower relative electrolytic leakage, and decreased stomatal aperture compared with LL. In addition, RNA-Seq was carried out on the two varieties under normal conditions and heat stress. The results revealed a total of 1485 up-regulated and 946 down-regulated genes under normal conditions, while 602 genes were up-regulated and 1212 genes were down-regulated under heat stress. Among these genes, several differentially expressed genes (DEGs) involved in the MAPK (mitogen-activated protein kinase) signaling pathway and members of bHLH (basic helix-loop-helix) transcription factors showed significant up- or down-regulation after heat stress. Next, to validate these findings, we conducted quantitative real-time PCR (qRT-PCR) analysis, which confirmed the expression patterns of the genes detected through RNA-Seq. Collectively, the DEGs between the two contrasting cultivars identified in our study provide novel insight into excavating helpful candidate genes associated with heat tolerance in bottle gourd. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Genetic characterization of plant genetic resources is a prerequisite for a strategic breeding programme. Bottle gourd is an important cucurbit grown throughout India for its tender fruits used for culinary purposes. For the present investigation, twenty-four genotypes of bottle gourd were collected from Eastern India to examine the magnitude of genetic diversity based on 16 quantitative traits and seven ISSR (Inter Simple Sequence Repeats) markers. In the morphological study, genotypes performed significantly in all traits; however, BRBG-65 (591.10 q/ha) proved to be a high yielder with other attributing traits in a pooled analysis of two growing seasons, compared to all three check cultivars of the region. Almost all the traits under study showed a high percentage of genotypic coefficient of variation, phenotypic coefficient of variation and heritability with genetic advance. Quantitative traits like fruit weight, number of fruits per vine and yield of marketable fruits per vine displayed a strong character association with the yield per hectare. The principal component analysis of quantitative traits depicted six principal components, accounting for 79.33% of the total variations. The first two components accounted for 44.92 cumulative percent of the total variation, signifying a high degree of correlation between the analyzed traits. In the molecular study, the average polymorphism percentage was obtained by the ISSR marker pattern studied by seven primers. Totally, 48 reproducible amplicons were generated, with an average of around 6.85 amplicons per primer. The values of polymorphic information content, major allele frequency, Nei's gene diversity, and Shannon's information index were obtained in a wide range. A wide range of values regarding Jaccard's similarity coefficient was also ensured through ISSR markers. Dendrogram and similarity matrix produced from ISSR data were then compared and confirmed the discrimination among genotypes within these clusters. Most genotypes studied in the present experiment differed according to their geographic origin. The investigation highlights the potential utilization of these germplasms for further genetic analysis. [ABSTRACT FROM AUTHOR]

Drying food is an effective method to preserve fruits and vegetables, increasing their shelf life and preventing germination. Bottle gourds, known for their high nutritional value, can rot quickly due to their high moisture content, causing biochemical and microbial changes. To improve shelf life, moisture can be removed to prevent microbial activity. Different drying methods are used to preserve gourd family vegetables for long periods and off-season. Indirect solar drying shields foods from UV rays, dust, insects, and other contamination by avoiding direct exposure. This study aimed to utilize an indirect solar dryer to research the potential for drying bottle gourds and conduct physicochemical analyses for the dried products to compare the drying techniques. In this experiment, different samples of bottle gourd slices were dried using an indirect solar dryer and compared with sun and shade drying. Under physicochemical properties, moisture content (MC), retention of color, thickness, and shrinkage of dried samples were analyzed and compared. The result showed that the time taken to reach 10% moisture was 13 h for samples dried with sun drying. While shade and indirect solar drying took 17 h. The Midilli-Kucuk model outperforms other approaches for drying bottle gourd, with the greatest R2 values (0.9967, 0.9950, 0.9936) and the lowest RMSE values (0.0182, 0.0220, 0.0277) for direct sun, shade, and indirect solar drying, respectively. The indirect sun dryer showed good color retention compared to the fresh bottle gourd samples with L* value of 44.08, b* value of 15.40 and a* value of 7.01. The volumetric shrinkage was 97.64% in the direct sun drying method after 15 h, which is higher compared to sun and shade dryers due to the effect of time and temperature. In addition, there was a significant difference (p-value

Lagenaria siceraria (bottle gourd) contains the wide range of chemical components such as minerals, vitamins, flavone-C glycosides, fatty acids and omega-3. In this study, nutritional composition and heavy metals content were analyzed in peel, flesh and cap of the bottle gourd. Flesh (356.85 g) of bottle gourd was showed maximum weight with maximum weight contribution (80.27 %). The cap of bottle gourd showed the maximum total carotenoids (305.00 ppm), TSC (29.56 mg/100 g) and TSS (0.580 ºBrix). Peel of bottle gourd represented significant antioxidant activity by RPA (2536.00 mg/100 g) and DPPH (36.43 % inhibition) with higher TPC (2819.88 mg/100 g) as well as TFC (60.28 mg/100 g). Fe was found maximum in three parts of bottle gourd than other metals concentration. The observed concentrations of heavy metals were found under the standard safe limits.

Fruit size is a crucial agronomic trait in bottle gourd, impacting both yield and utility. Despite its significance, the regulatory mechanism governing fruit size in bottle gourd remains largely unknown. In this study, we used bottle gourd (small-fruited H28 and large-fruited H17) parent plants to measure the width and length of fruits at various developmental stages, revealing a single ‘S’ growth curve for fruit expansion. Paraffin section observations indicated that both cell number and size significantly influence bottle gourd fruit size. Through bulked segregant analysis and combined genotype–phenotype analysis, the candidate interval regulating fruit size was pinpointed to 17,747,353 bp–18,185,825 bp on chromosome 9, encompassing 0.44 Mb and including 44 genes. Parental fruits in the rapid expansion stage were subjected to RNA-seq, highlighting that differentially expressed genes were mainly enriched in pathways related to cell wall biosynthesis, sugar metabolism, and hormone signaling. Transcriptome and resequencing analysis, combined with gene function annotation, identified six genes within the localized region as potential regulators of fruit size. This study not only maps the candidate interval of genes influencing fruit size in bottle gourd through forward genetics, but also offers new insights into the potential molecular mechanisms underlying this trait through transcriptome analysis.

Cucurbitaceous crops, one of the main crops of agriculture, are sensitive to many plant viruses. In August 2019, virus-like symptoms were observed on some cucurbit plants grown in private home gardens in Antalya and Denizli provinces (Turkey). A total of 53 leaf samples were sampled from plants with the most symptoms (melon (Cucumis melo L.), watermelon (Citrullus lanatus L.), bottle gourd (Lagenaria siceraria (Molina) Standl.), and snake melon (Cucumis melo var. flexuosus) and tested by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) against possible watermelon mosaic potyvirus (WMV) infection. The coat protein gene (CP) specific primer sets amplified a gene product of nearly 820 bp fragment from symptomatic plants. WMV infections were detected in 31 individual cucurbit plants, including 11 melons, 8 watermelons, 7 snake melons and 5 bottle gourds. The presence of viral infection was found only in ornamental squash plants in Antalya province and in all cucurbits sampled in Denizli province. To better comprehend the molecular characteristics of virus isolates, the amplified viral DNA fragments were cloned in a proper prokaryotic plasmid, sequenced by Next Generation Sequencing (NGS) and recorded to GenBank. Bioinformatic analyses using the Basic Local Alignment Search Tool (BLAST) showed that the identified CP gene sequences exhibited significant nucleotide homogeneity, supported by a high nucleotide similarity index with that of other isolates around the world. In addition, Turkish isolates isolated from Antalya and Denizli regions showed approximately 94% nucleotide similarity among themselves. For phylogenetic inference, WMV sequences were subjected to multiple alignments with isolates from different geographic origins of the same viruses. Molecular phylogeny showed that all WMV isolates are closely related to other world WMV isolates at variable rates. WMV is wide host range viruses in cucurbit crops, however, this work is the first scientific report of WMV isolates detected in bottle gourd and snake melon from the South and West Regions of Turkey all over the world. [ABSTRACT FROM AUTHOR]

The morpho-yield and quality traits of bottle gourd varieties were tested in response to nitrogen (N) nutrition during the year 2020 at the horticulture research area of Sindh Agriculture University Tandojam. The study was performed by applying Randomized complete block design (RCBD) with three replications. The two varieties Anmol and Long green were tested by applying six N rates that included (0 Kg N, (control), 50, 75, 100, 125 and 150 Kg N ha-1). Both the varieties exhibited positive response to nitrogen fertilization. The maximum vine length, branches vine-1 were noted in plants, fertilized with increasing N rates (150 and 125 kg ha-1). In contrast shorter plants with less branches were noted when they were not fertilized with N nutrition.). The N nutrition also had a positive effect on fruit traits. The maximum fruit number with greater length and weight was recorded in plants supplied with increasing N fertilization. The N fertilization had beneficial influence on plant quality characteristics. The maximum pH, TSS, moisture and ash content in plants were observed, nourished with increasing N supply. The varietal comparison indicates that fruits of "Long green" had lower pH (5.88 vs. 6.0), higher in TSS (5.39 vs. 4.28%), moisture (67.74 vs. 55.52%), ash contents (19.96 vs. 19.01%), fruit yield (18178 vs. 17123 kg ha-1) than variety "Anmol". In general, vines fertilized at 150 kg ha-1 N performed better than those fertilized at lower N levels for entire studied attributes, and "Long green" did better than "Anmol" among the evaluated varieties. The data clearly indicated severe soil N deficiency; and with each increment in applied N, the crop response was positive and significant (p<0.05). Hence, the farmers are suggested that "Long Green" bottle gourd variety may preferably be adapted with N at 150 kg ha-1 due to intense performance under agro-ecological conditions of Tandojam Sindh, Pakistan. [ABSTRACT FROM AUTHOR]

While conducting field trial of 82 genotypes of bottle gourd at Delhi during 2020–2021, a particular genotype, IC-0262269 was found to be affected by chlorotic curly stunt disease (CCSD). The affected plants were severely stunted and bearing very small chlorotic and crinkle leaves. The disease incidence in the said genotype was as high as 80% among different replicated trial blocks. The application of PCR using a generic primers specific to begomoviruses, as well as species-specific PCR diagnostics to six tomato-infecting begomoviruses: tomato leaf curl New Delhi virus (ToLCNDV), tomato leaf curl Palampur virus (ToLCPalV), tomato leaf curl Joydebpur virus (ToLCJoV), tomato leaf curl Gujrat virus (ToLCGuV), tomato leaf curl Bangalore virus (ToLCBV), and chilli leaf curl virus (ChiLCV) showed that, only ToLCPalV could be detected in the genotype IC-0262269. Following, rolling circle amplification, cloning and sequencing of full-length DNA-A and DNA-B genome of an isolate BoG1-ND from the genotype IC-0262269 revealed association of ToLCPalV with the disease. The successful agro-infection of the cloned genome of BoG1-ND (DNA-A and DNA-B) in the plants of Nicotiana benthamiana and bottle gourd demonstrated that ToLCPalV is the causal begomovirus of CCSD. The study provides the first evidence of the natural occurrence of ToLCPalV in bottle gourd crop and also showed that the bottle gourd genotype IC-0262269 is super-susceptible to ToLCPalV. [ABSTRACT FROM AUTHOR]

Cucumber green mottle mosaic virus (CGMMV) is a typical seed-borne tobamovirus that mainly infects cucurbit crops. Due to the rapid growth of international trade, CGMMV has spread worldwide and become a significant threat to cucurbit industry. Despite various studies focusing on the interaction between CGMMV and host plants, the molecular mechanism of CGMMV infection is still unclear. In this study, we utilized transcriptome and metabolome analyses to investigate the antiviral response of bottle gourd (Lagenaria siceraria) under CGMMV stress. The transcriptome analysis revealed that in comparison to mock-inoculated bottle gourd, 1929 differently expressed genes (DEGs) were identified in CGMMV-inoculated bottle gourd. Among them, 1397 genes were upregulated while 532 genes were downregulated. KEGG pathway enrichment indicated that the DEGs were mainly involved in pathways including the metabolic pathway, the biosynthesis of secondary metabolites, plant hormone signal transduction, plant–pathogen interaction, and starch and sucrose metabolism. The metabolome result showed that there were 76 differentially accumulated metabolites (DAMs), of which 69 metabolites were up-accumulated, and 7 metabolites were down-accumulated. These DAMs were clustered into several pathways, including biosynthesis of secondary metabolites, tyrosine metabolism, flavonoid biosynthesis, carbon metabolism, and plant hormone signal transduction. Combining the transcriptome and metabolome results, the genes and metabolites involved in the jasmonic acid and its derivatives (JAs) synthesis pathway were significantly induced upon CGMMV infection. The silencing of the allene oxide synthase (AOS) gene, which is the key gene involved in JAs synthesis, reduced CGMMV accumulation. These findings suggest that JAs may facilitate CGMMV infection in bottle gourd. [ABSTRACT FROM AUTHOR]

Bottle gourd (Lagenaria siceraria) is a well-known cucurbit with an active functional ingredient. A two-year field experiment was carried out at the Research Farm of Seed Science and Technology, CCS HAU, Hisar, in a randomized block design during the Kharif season (Kharif is one of the two major cropping seasons in India and other South Asian countries, heavily reliant on monsoon rains with the other being Rabi) and the summer season. Five different crossing periods (CP), viz. CP1, CP2, CP3, CP4, and CP5, were considered to illustrate the effects of agro-climatic conditions on the quality and biochemical components of two bottle gourd parental lines and one hybrid, HBGH-35. The average mean temperature for the Kharif season in 2017 was 31.7 °C, and for the summer season, it was 40.1 °C. Flowers were tagged weekly from the start of the crossing period until the end and harvested separately at different times. The fruits harvested from different crossing periods under different environmental conditions influenced the bottle gourd's qualitative and biochemical traits and showed significant variations among the five crossing period environments. A positive significance and correlation were observed between weather variables and different biochemical characteristics. Henceforth, the CP4 crossing period at a temperature of 31.7 °C retained high-quality seed development, which may be essential in enhancing agricultural productivity and the national economy. [ABSTRACT FROM AUTHOR]

Bottle gourd (Lagenaria siceraria L.) belongs to the cucurbit family and has a long history of cultivation in tropical and subtropical regions worldwide, both for food and medicine. Popularized by its unique fruit shapes, gourds are used to make ornaments and musical instruments. However, there is limited information on volatile organic compounds (VOCs) in the bottle gourd fruit. In the present study, we conducted a comparative analysis of VOCs profiled in two accessions (USVL5 and USVL10) with distinct fruit shapes: bottle and cylinder. While USVL5 only produced long cylinder fruits, USVL10 produced two fruit types, cylinder (USVL10CYN) and bottle (USVL10A and USVL10B). VOCs in each line were analyzed using headspace solid-phase microextraction–gas chromatography/mass spectrometry (HS-SPME-GC/MS). Aliphatic aldehydes and alcohols were the most abundant compounds found in these bottle gourd accessions. Based on the functional profile of the identified VOCs, our results reveal the suitability of our tested line (USVL10), enriched in functionally important VOCs such as hexanal (abundance = 381.07), nonanal (abundance = 9.85), 2-methoxy-2-methylpropane (abundance = 21.26) and D-limonene (abundance = 31.48). The VOCs profiling and functional analyses support the notion that the bottle gourd accession USVL10 can be a good candidate for its use in agriculture, the health care industry and domestic uses. [ABSTRACT FROM AUTHOR]

The development of highly nutritious bakery products with optimum utilization of food waste is a major challenge for the food industry. The optimum utilization of food waste for the sustainable development goal of the country is important for the growth of the nation. The aim of the present work is to prepare value-added composite flour-mixed bread from waste fruit and vegetables. The composite flour was prepared in four formulations of peel and pomace with wheat flour (PPWF), as PPWF1, PPWF2, PPWF3, and PPWF4. Composite flour was blended with a mix of vegetable and fruit pomace powders and whole wheat flour. Indian gooseberry pomace powder, apple pomace powder, bottle gourd peel powder, and potato peel powder were used with whole wheat flour to make pomace and whole wheat flour compositions such as PPWF1, PPWF2, PPWF3, and PPWF4. Out of these four flours, PPWF3 contained a good amount of fiber 8.16%, crude protein 3.18%, total phenolic content 14.48%, moisture 9.5%, vitamin C 13.64 mg/100 g, and total phenolic compound 14.48 (mg/GAE/g), which are maximum and acceptable range values as compared to the other three composite flours and the control group flour. PPWF3 is used as a partial replacement ratio for wheat flour due to its high phenolic content, vitamin C content, and richness in fibers. This composite flour is used to make bread dough, and two samples, G1 and G2, are made, out of which G2 offers better nutritional, functional, and sensory evaluations in comparison with refined wheat bread, which is taken as a control group. Thus, such utilization of food waste in bread making can generate value from waste and improve the nutritional attributes of bread, which may improve an individual's health. [ABSTRACT FROM AUTHOR]

Bottle gourd [Lagenaria siceraria (Molina) Standl.] is cultivated for multiple utilities, including as a leafy vegetable, for fresh and dried fruits and seeds. It is an under-researched and -utilized crop, and modern varieties are yet to be developed and deployed in sub-Saharan Africa (SSA). There is a dire need for pre-breeding and breeding of bottle gourds for commercialization in SSA. Therefore, this study aimed to determine the combining ability and heterosis among selected genotypes of bottle gourd for fruit yield and related traits under drought-stressed and non-stressed conditions to select the best parents and hybrids. Eight preliminarily selected and contrasting parents with drought tolerance were crossed using a half-diallel mating design. The 8 parents and 28 crosses were evaluated under non-stressed (NS) and drought-stressed (DS) conditions across two growing seasons (2020/21 and 2021/22) using a 6 × 6 alpha lattice design with three replicates. Data were collected on fruit yield and related traits and subjected to analysis of variance, combining ability and heterosis analyses. Significant (p < 0.05) specific combining ability (SCA) and general combining ability (GCA) effects were computed for fruit yield per plant (FYPP). The SCA × environment and GCA × environment interaction effects were highly significant (p < 0.001) for FYPP and SYPP. The results suggest that genetic effects were affected by the test environment. Parental genotypes BG-58 and GC recorded positive and significant GCA effects for FYPP under the DS condition, whereas GC recorded positive and significant GCA effects for FYPP under the NS condition. The two genotypes are ideal breeding parents for population development to select genotypes with high fruit and seed yields. Crosses BG-27 × BG-79, BG-79 × BG-52, BG-79 × BG-70, BG-80 × BG-70, BG-80 × GC, and BG-70 × GC recorded high and positive SCA effects for FYPP and SYPP under DS condition. Crosses BG-81 × BG-52, BG-81 × GC, BG-27 × BG-79, BG-27 × GC, BG-79 × GC, BG-80 × BG-70, BG-81 × BG-58, BG-27 × BG-80, BG-27 × BG-58, BG-79 × BG-52, BG-52 × BG-58, BG-80 × BG-58, and BG-58 × BG-70 recorded high and positive SCA effects for FYPP and SYPP under NS condition. Crosses BG-80 × BG-58, BG-27 × BG-79, BG-79 × BG-52, BG-27 × BG-52, and BG-52 × BG-80 showed high and positive mid- and better-parent heterosis under DS condition for FYPP and SYPP. Crosses BG-27 × GC, BG-79 × GC, BG-27 × BG-58, and BG-27 × BG-79 showed high and positive mid- and better parent heterosis under NS condition for FYPP and SYPP. The newly selected families are recommended for multi-environment evaluation forrelease and commercialization in South Africa or similar agroecologies. [ABSTRACT FROM AUTHOR]

Bottle gourd, a common vegetable in the human diet, has been valued for its medicinal and energetic properties. In this experiment, the time-resolved analysis of the changes in the proteins’ electrophoretic patterning of the seed development at different crossing periods was studied in bottle gourd using label-free quantitative proteomics. Hybrid HBGH-35 had the highest observed protein levels at the 4th week of the crossing period (F4) compared to the parental lines, viz. G-2 (M) and Pusa Naveen (F). The crossing period is significantly correlated with grain filling and reserve accumulation. The observed protein expression profile after storage was related to seed maturation and grain filling in bottle gourds. A total of 2517 proteins were identified in differentially treated bottle gourd fruits, and 372 proteins were differentially expressed between different crossing periods. Proteins related to carbohydrate and energy metabolism, anthocyanin biosynthesis, cell stress response, and fruit firmness were characterized and quantified. Some proteins were involved in the development, while others were engaged in desiccation and the early grain-filling stage. F4 was distinguished by an increase in the accumulation of low molecular weight proteins and enzymes such as amylase, a serine protease, and trypsin inhibitors. The seed vigor also followed similar patterns of differential expression of seed storage proteins. Our findings defined a new window during seed production, which showed that at F4, maximum photosynthetic assimilates accumulated, resulting in an enhanced source–sink relationship and improved seed production. Our study attempts to observe the protein expression profiling pattern under different crossing periods using label-free quantitative proteomics in bottle gourd. It will facilitate future detailed investigation of the protein associated with quality traits and the agronomic importance of bottle gourd through selective breeding programs.

Cucumber green mottle mosaic virus (CGMMV, genus Tobamovirus) is a widely occurring tobamovirus in cucurbits. The genome of CGMMV has been used previously for the expression of foreign genes in the plant. High throughput delivery and high viral titer are important requirements of foreign protein expression in plant through virus genome-based vector, in this study, Agrobacterium containing infectious construct of CGMMV was infiltrated through syringe, vacuum and high-speed spray to N. benthamiana, cucumber and bottle gourd leaves. The success rate of systemic infection of CGMMV agro-construct through all three methods was higher (80–100%) in N. benthamiana compared to the cucurbits (40–73.3%). To determine the high-throughput delivery of CGMMV in the plant system, four delivery methods viz. rubbing, syringe infiltration, vacuum infiltration and high-speed spray using the progeny virus derived through CGMMV agro-construct were compared in the three different plant species. Based on the rate of systemic infection and time required to perform delivery by different methods, vacuum infiltration was found most efficient for the high-throughput delivery of CGMMV. The quantification of CGMMV through qPCR revealed that CGMMV load varied considerably in leaf and fruit tissues depending with the time of infection. Immediately after expression of symptoms, a high load of CGMMV (~ 1 µg/100 mg of tissues) was noticed in young leaves of N. benthamiana and cucumber. In bottle gourd leaves, the CGMMV load was far low compared to N. benthamiana and cucumber plants. In the fruit tissues of cucumber and bottle gourd higher virus load was observed in mature fruit but not in immature fruit. The findings of the present study will serve as an important base line information to produce foreign protein through CGMMV genome-vector. [ABSTRACT FROM AUTHOR]

Investigations were carried out to identify the source of resistance in 67 bottle gourd genotypes for gummy stem blight, powdery mildew and cucumber green mottle mosaic virus (CGMMV) diseases, under natural field epiphytotic conditions. The genotypes BG-95 (105.13), BG-114-1 (131.04), BG-114-3 (208.81) and BG-77-6-1 (221.80) were resistant for gummy stem blight with low AUDPC values, while, BG-125-5 (232.22), BG-6-3 found (250.00), BG-125-4 (307.78), BG-8-1 (308.89), BG-125-2 (311.11) and BG-124-2 (423.33) resistant with low AUDPC values for powdery mildew. Further, the two genotypes such as IIHR-19 and BG131 showed field level resistance against CGMMV. The selected genotypes based on field evaluation were subjected for artificial screening under glass house conditions. The genotypes, recorded consistent resistant reactions were BG-114-3, BG-77-6-1 and BG-95 for gummy stem blight disease and BG-6-3, BG-8-1, BG-125-4 and BG-125-2 for powdery mildew. The stable and durable source of resistance identified for gummy stem blight and powdery mildew in bottle gourd genotypes will hasten the process of developing resistance varieties in bottle gourd. [ABSTRACT FROM AUTHOR]

Powdery mildew (PM) is the main disease that afflicts bottle gourd. Previous studies on PM mainly focused on its effects on pumpkin, melon, and other crops; however, the exact molecular mechanism of bottle gourd resistance to PM remains unclear. RNA sequencing (RNA-Seq) technology was used to investigate the dynamic changes in leaf transcriptome profiles between resistant and susceptible gourd at 12, 24, 48, and 72 h post-inoculation with powdery mildew. Compared with a susceptible variety (G3), the expression levels of the differentially expressed genes of phenylpropanoid biosynthesis, starch, and sucrose metabolism, and plant–pathogen interaction pathways in disease-resistant plants were upregulated. We propose that disease resistance and tolerance in bottle gourd are enhanced via several pathways, including the antioxidant system, phenylalanine biosynthesis, and cell wall cellulose synthesis. Our research will provide an important basis for further screening and breeding PM resistance in bottle gourd. [ABSTRACT FROM AUTHOR]

Bottle gourd pomace, a waste from vegetable processing industry was used to prepare instant kheer (dessert) mix. In this study, the bottle gourd was procured from the farm, washed, grated, steam blanched and the grits were further divided into two parts. One part of grits was dried without juice extraction (BGFD- Bottle gourd fresh dried), while, the other half (BGPD- Bottle gourd pomace dried) was dehydrated after extraction of juice. The dehydrated grits were used for the preparation of kheer mix and the recipe was optimized using RSM Central Composite Design (CCD). The variables were BGFD and BGPD ranged 3–7 g. The other ingredients with the fixed quantities were milk powder (50 g), sugar (15 g), and small cardamom (1 g). The product was selected based on sensory responses like taste, colour, flavour, texture, and overall acceptability (OAA). The software suggested a kheer mix prepared using 7 g BGFD and 3 g BGPD will produce the best sensory scores. The prepared kheer mix had a moisture, TSS, carbohydrates, reducing sugars, total sugars, titratable acidity, crude protein, and crude fat content of 7.9%, 27 °B, 72.21%, 10.79%, 16.75%, 0.896% CA, 10.76%, and 7.63%, respectively. The product was rich in energy (400.55 kcal/100 g), total phenols (4.99 mg/100 g), and exhibited strong antioxidant activity (46%). The total plate count on the product on nutrient agar medium was 4.3 × 106 CFU/g. The kheer could be prepared by adding 140 mL of water to 70 g of water to kheer mix and cooking it for 10 min. Further, to see the credibility and obtain more clearer patterns, the Canonical Correlation Analysis (CCA) and Principal Component Analysis (PCA) were applied. The overall variation of the BGFD and BGPD on the sensory parameters based on canonical correlation analysis was 92.5%. The sum of Principal Components PC1 and PC2 explained a very high variability (98.2%) among the studied treatments.