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3. List of Contributors

Author

Akinyi, Teckla, primary, Balland, Martial, additional, Barriga, Elias H., additional, Betz, Timo, additional, Bhatt, Manish, additional, Borja, Cristian, additional, Boudou, Thomas, additional, Brochard-Wyart, Françoise, additional, Cappello, Giovanni, additional, Catheline, Stefan, additional, Clark, Andrew G., additional, Cloutier, Guy, additional, Espina, Jaime A., additional, Gonzalez-Rodriguez, David, additional, Grasland-Mongrain, Pol, additional, Guevorkian, Karine, additional, Milivojevic, Milan, additional, Moral, Elena, additional, Muñoz, Jose J., additional, Pajic-Lijakovic, Ivana, additional, Petrolli, Vanni, additional, and Saraiva, Joana E., additional

Published
2021
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5. In vivo measurement of human brain elasticity using a light aspiration device

Author

Schiavone, Patrick, Chassat, Fabrice, Boudou, Thomas, Promayon, Emmanuel, Valdivia, F., and Payan, Yohan

Subjects
Physics - Medical Physics
Abstract

The brain deformation that occurs during neurosurgery is a serious issue impacting the patient "safety" as well as the invasiveness of the brain surgery. Model-driven compensation is a realistic and efficient solution to solve this problem. However, a vital issue is the lack of reliable and easily obtainable patient-specific mechanical characteristics of the brain which, according to clinicians' experience, can vary considerably. We designed an aspiration device that is able to meet the very rigorous sterilization and handling process imposed during surgery, and especially neurosurgery. The device, which has no electronic component, is simple, light and can be considered as an ancillary instrument. The deformation of the aspirated tissue is imaged via a mirror using an external camera. This paper describes the experimental setup as well as its use during a specific neurosurgery. The experimental data was used to calibrate a continuous model. We show that we were able to extract an in vivo constitutive law of the brain elasticity: thus for the first time, measurements are carried out per-operatively on the patient, just before the resection of the brain parenchyma. This paper discloses the results of a difficult experiment and provide for the first time in-vivo data on human brain elasticity. The results point out the softness as well as the highly non-linear behavior of the brain tissue., Comment: Medical Image Analysis (2009) accept\'e

Published
2009

6. Force propagation between epithelial cells depends on active coupling and mechano-structural polarization

Author

Ruppel, Artur, primary, Wörthmüller, Dennis, primary, Misiak, Vladimir, additional, Kelkar, Manasi, additional, Wang, Irène, additional, Moreau, Philippe, additional, Méry, Adrien, additional, Révilloud, Jean, additional, Charras, Guillaume, additional, Cappello, Giovanni, additional, Boudou, Thomas, additional, Schwarz, Ulrich S, additional, and Balland, Martial, additional

Published
2023
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7. Author response: Force propagation between epithelial cells depends on active coupling and mechano-structural polarization

Author

Ruppel, Artur, primary, Wörthmüller, Dennis, primary, Misiak, Vladimir, additional, Kelkar, Manasi, additional, Wang, Irène, additional, Moreau, Philippe, additional, Méry, Adrien, additional, Révilloud, Jean, additional, Charras, Guillaume, additional, Cappello, Giovanni, additional, Boudou, Thomas, additional, Schwarz, Ulrich S, additional, and Balland, Martial, additional

Published
2023
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14. Force propagation between epithelial cells depends on active coupling and mechano-structural polarization

Author

Ruppel, Artur, primary, Wörthmüller, Dennis, additional, Misiak, Vladimir, additional, Kelkar, Manasi, additional, Wang, Irène, additional, Moreau, Philippe, additional, Méry, Adrien, additional, Révilloud, Jean, additional, Charras, Guillaume, additional, Cappello, Giovanni, additional, Boudou, Thomas, additional, Schwarz, Ulrich S., additional, and Balland, Martial, additional

Published
2022
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29. Confinement-Induced Transition between Wavelike Collective Cell Migration Modes

Author

Petrolli, Vanni, primary, Le Goff, Magali, additional, Tadrous, Monika, additional, Martens, Kirsten, additional, Allier, Cédric, additional, Mandula, Ondrej, additional, Hervé, Lionel, additional, Henkes, Silke, additional, Sknepnek, Rastko, additional, Boudou, Thomas, additional, Cappello, Giovanni, additional, and Balland, Martial, additional

Published
2019
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30. Confinement-induced transition between wave-like collective cell migration modes

Author

Petrolli, Vanni, primary, Goff, Magali Le, additional, Tadrous, Monika, additional, Martens, Kirsten, additional, Allier, Cédric, additional, Mandula, Ondrej, additional, Hervé, Lionel, additional, Henkes, Silke, additional, Sknepnek, Rastko, additional, Boudou, Thomas, additional, Cappello, Giovanni, additional, and Balland, Martial, additional

Published
2018
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31. Magnetic approaches to study collective three-dimensional cell mechanics in long-term cultures (invited).

Author

Zhao, Ruogang, Boudou, Thomas, Wei-Gang Wang, Chen, Christopher S., and Reich, Daniel H.

Subjects
*EXTRACELLULAR matrix, *FIBROBLASTS, *CELL culture, *EXTRACELLULAR matrix proteins, *CULTURES (Biology)
Abstract

Contractile forces generated by cells and the stiffness of the surrounding extracellular matrix are two central mechanical factors that regulate cell function. To characterize the dynamic evolution of these two mechanical parameters during tissue morphogenesis, we developed a magnetically actuated micro-mechanical testing system in which fibroblast-populated collagen microtissues formed spontaneously in arrays of microwells that each contains a pair of elastomeric microcantilevers. We characterized the magnetic actuation performance of this system and evaluated its capacity to support long-term cell culture. We showed that cells in the microtissues remained viable during prolonged culture periods of up to 15 days, and that the mechanical properties of the microtissues reached and maintained at a stable state after a fast initial increase stage. Together, these findings demonstrate the utility of this microfabricated bio-magneto-mechanical system in extended mechanobiological studies in a physiologically relevant 3D environment. [ABSTRACT FROM AUTHOR]

Published
2014
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32. Signal mingle: Micropatterns of BMP-2 and fibronectin on soft biopolymeric films regulate myoblast shape and SMAD signaling

Author

Fitzpatrick, Vincent, Fourel, Laure, Destaing, Olivier, Gilde, Flora, Albigès-Rizo, Corinne, Picart, Catherine, Boudou, Thomas, Laboratoire des matériaux et du génie physique (LMGP ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])

Subjects
animal structures, [SDV]Life Sciences [q-bio], Cell Culture Techniques, Bone Morphogenetic Protein 2, Smad Proteins, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Article, Cell Line, Fibronectins, Myoblasts, Mice, Protein Transport, embryonic structures, Cell Adhesion, Animals, Protein Binding, Signal Transduction
Abstract

International audience; In vivo, bone morphogenetic protein 2 (BMP-2) exists both in solution and bound to the extracellular matrix (ECM). While these two modes of presentation are known to influence cell behavior distinctly, their role in the niche microenvironment and their functional relevance in the genesis of a biological response has sparsely been investigated at a cellular level. Here we used the natural affinity of BMP-2 for fibronectin (FN) to engineer cell-sized micropatterns of BMP-2. This technique allowed the simultaneous control of the spatial presentation of fibronectin-bound BMP-2 and cell spreading. These micropatterns induced a specific actin and adhesion organization around the nucleus, and triggered the phosphorylation and nuclear translocation of SMAD1/5/8 in C2C12 myoblasts and mesenchymal stem cells, an early indicator of their osteoblastic trans-differentiation. We found that cell spreading itself potentiated a BMP-2-dependent phosphorylation of SMAD1/5/8. Finally, we demonstrated that FN/BMP-2-mediated early SMAD signaling depended on LIM kinase 2 and ROCK, rather than myosin II activation. Altogether, our results show that FN/BMP-2 micropatterns are a useful tool to study the mechanisms underlying BMP-2-mediated mechanotransduction. More broadly, our approach could be adapted to other combinations of ECM proteins and growth factors, opening an exciting avenue to recreate tissue-specific niches in vitro. Due to their physiological relevance, bone morphogenetic proteins (BMPs) are widely studied for orthopedic clinical applications to enhance the healing of large bone defects 1,2 , as well as for developing new strategies in bone tissue engineering 3-5. BMPs are indeed highly potent growth factors (GFs) that play a crucial role in mor-phogenesis and tissue homeostasis during embryonic development and until adulthood 6,7. In particular, BMP-2 promotes the differentiation of mesenchymal stem cells (MSCs) and osteoblasts toward osteocytes 8,9 , and induces the trans-differentiation of myoblasts into osteoblasts 10. In addition, BMP-2 in solution plays a role in early adhesive events, including adhesion and migration through cytoskeletal reorganization 11,12. Recently, several studies have demonstrated that BMP-2 strongly interacts with extra-cellular matrix (ECM) proteins 13 , especially fibronectin (FN) due to its highly promiscuous GF-binding site (the 12th to 14th type III repeats) 14,15. Moreover, immobilized BMP-2 whether by physical adsorption (i.e. matrix-bound BMP-2 13,16) or by covalent grafting 17 was shown to regulate cell behavior quite distinctly from BMP-2 in solution. This effect is currently poorly known and is likely due to the close proximity and crosstalk of integrin-binding domains of FN and BMP-2 13,17-19. It has indeed been shown that the secretion of FN by cells is necessary for BMP-2-mediated signaling 13. A consequence of this association of BMP-2 with ECM proteins in vivo is that the spatially patterned presentation of BMPs by

Published
2017

33. Additional file 1: of Quiescence of human muscle stem cells is favored by culture on natural biopolymeric films

Author

Monge, Claire, DiStasio, Nicholas, Rossi, Thomas, Sébastien, Muriel, Sakai, Hiroshi, Benoit Kalman, Boudou, Thomas, Shahragim Tajbakhsh, Marty, Isabelle, Bigot, Anne, Mouly, Vincent, and Picart, Catherine

Subjects
nervous system, integumentary system, sense organs, tissues
Abstract

Figure S1. SCs are alive after 21 days of culture. Calcein/EthD (Live/dead) assay on SCs during 21 days on EDC10 films without any change/refreshing of GM. Scale bar: 100 µm. Figure S2. SCs secrete more Collagen I on plastic than on EDC10 films. Immunostaining of fibronectin and collagen I in SCs cultured 21 days on EDC10 films, 3 days on EDC70 fil ms and plastic. Actin was stained by rhodamine-phalloidin. Scale bar: 200 µm. Figure S3. Images of the cells corresponding to Figure 5, for DAPI and Pax7 staining.

Published
2017
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34. In vitro microphysiological system for high throughput 3D tissue organization and biological function

Author

Chen, Christopher S., Margulies, Kenneth B., Boudou, Thomas, Legant, Wesley R., Yang, Michael, Department of Bioengineering [Philadelphia], University of Pennsylvania [Philadelphia], Department of Cell and Developmental Biology, University of Pennsylvania, Laboratoire des matériaux et du génie physique (LMGP ), and Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.IB]Life Sciences [q-bio]/Bioengineering
Abstract

Techniques for generating microtissues, including a micro-fabricated platform including at least one micro-well including a plurality of micro-cantilevers coupled thereto and surrounded by a plurality of ridges, each micro-cantilever including a cap at a terminal end thereof. The platform can be immersed in a suspension of cells. The suspension of cells can be driven into at least one micro-well, and the ridges can be de-wetted to remove excess suspension and isolate the suspension of cells in each micro-well. The cells can be driven in the suspension of each micro-well toward a top surface of the suspension, which can be polymerized to form a matrix. The cells can be cultivated to spontaneously compact the matrix such that the micro-cantilevers anchor and constrain the contracting matrix to form a band of microtissue that spans across the micro-cantilevers.

Published
2016

35. Engineering 3D microtissues

Author

Kalman, Benoît, Monge, Claire, Picart, Catherine, Boudou, Thomas, Laboratoire des matériaux et du génie physique (LMGP ), and Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.MATE]Chemical Sciences/Material chemistry, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

36. Matrix-Bound Presentation of Bone Morphogenetic Protein 2 by Multilayer Films: Fundamental Studies and Applicationsto Orthopedics

Author

Gilde, Flora, Guillot, Raphaël, Fourel, Laure, Almodóvar, Jorge, Crouzier, Thomas, Boudou, Thomas, Picart, Catherine, Laboratoire des matériaux et du génie physique (LMGP ), Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Boudou, Thomas

Subjects
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

37. Multiscale Porosity Directs Bone Regeneration in Biphasic Calcium Phosphate Scaffolds

Author

Rustom, Laurence E., Boudou, Thomas, Nemke, Brett W., Lu, Yan, Hoelzle, David J., Markel, Mark D., Picart, Catherine, and Wagoner Johnson, Amy J.

Abstract

Large and load-bearing bone defects are challenging to treat and cause pain and disfigurement. The design of efficacious bone scaffolds for the repair of such defects involves a range of length scales from the centimeter down to the micrometer-scale. Here, we assess the influence on bone regeneration of scaffold rod spacing (>300 μm) and microporosity (<50 μm), as well as the combination of different structures and materials in the same scaffold, i.e., at the millimeter scale. We use four single-domain scaffolds, microporous (MP) or nonmicroporous (NMP) and with either a “small” or “large” rod spacing. Multidomain scaffolds combine four regions corresponding to the macro- and microarchitectures of the single-domain scaffolds. The scaffolds are implanted in pig mandibles for 3 weeks and bone regeneration is assessed by measuring the average bone volume fraction, BVF̅, the bone distribution and the trabecular thickness from micro-CT data. For the single-domain scaffolds, BVF̅was 45 ± 3% for MP-small, 39 ± 2% for MP-large, 25 ± 2% for NMP-small, and 25 ± 2% for NMP-large. MP scaffolds have significantly higher BVF̅and a more uniform bone distribution compared to NMP, regardless of rod spacing. The average trabecular thickness is significantly larger in MP compared to NMP, and in “large” compared to “small” scaffolds. Microporosity affects trabecular thickness throughout the scaffold, while rod spacing affects it only at the scaffold periphery. In multidomain scaffolds, MP-large and NMP-large domains have similar BVF̅as compared to their respective single-domain counterparts. These results suggest that combining different architectures into one scaffold conserves the properties of each domain. Hence, bone growth and morphology can be tailored by controlling scaffold architecture from the millimeter down to the micrometer level. This will allow the customization of scaffold designs for the treatment of large and load-bearing bone defects.

Published
2024
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38. Magneto-Active Substrates for Local Mechanical Stimulation of Living Cells

Author

Coullomb, Alexis E., primary, Bidan, Cecile M., additional, Fratzl, Mario, additional, Moreau, Philippe, additional, Lombard, Alain H., additional, Wang, Irene, additional, Balland, Martial, additional, Boudou, Thomas, additional, Dempsey, Nora M., additional, Devillers, Thibaut, additional, and Dupont, Aurelie, additional

Published
2018
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39. Magneto-active substrates for local mechanical stimulation of living cells

Author

Bidan, Cécile M., primary, Fratzl, Mario, additional, Coullomb, Alexis, additional, Moreau, Philippe, additional, Lombard, Alain H., additional, Wang, Irène, additional, Balland, Martial, additional, Boudou, Thomas, additional, Dempsey, Nora M., additional, Devillers, Thibaut, additional, and Dupont, Aurélie, additional

Published
2018
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40. Magneto-active substrates for local mechanical stimulation of living cells

Author

Bidan, Cécile M., primary, Fratzl, Mario, additional, Coullomb, Alexis, additional, Moreau, Philippe, additional, Lombard, Alain H., additional, Wang, Irène, additional, Balland, Martial, additional, Boudou, Thomas, additional, Dempsey, Nora M., additional, Devillers, Thibaut, additional, and Dupont, Aurélie, additional

Published
2017
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42. How cells internalize a growth factor presented by a biomaterial: BMP-2 delivery to cells in a matrix-bound manner using layer-by-layer films

Author

Picart Catherine, Gilde Flora, Fourel Laure, Guillot Raphael, Paintrand Isabelle, Okada Takaharu, Boudou Thomas, and Albigez-Rizo Corinne

Subjects
Histology, Chemistry, Growth factor, medicine.medical_treatment, Layer by layer, Biomedical Engineering, Biophysics, medicine, Biomaterial, Bioengineering, Matrix bound, Bone morphogenetic protein 2, Biotechnology
Published
2016

45. Amyloid-like aggregates formation by blood plasma fibronectin

Author

Bascetin, Rümeyza, primary, Admane, Khadija, additional, Agniel, Rémy, additional, Boudou, Thomas, additional, Doussineau, Tristan, additional, Antoine, Rodolphe, additional, Gallet, Olivier, additional, Leroy-Dudal, Johanne, additional, and Vendrely, Charlotte, additional

Published
2017
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49. Micropatterns of BMP-2 and fibronectin regulate myoblast shape and SMAD signaling

Author

Fitzpatrick, Vincent, Fourel, Laure, Destaing, Olivier, Albigès-Rizo, Corinne, Picart, Catherine, Boudou, Thomas, Laboratoire des matériaux et du génie physique (LMGP ), Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Dynamique des systèmes d'adhérence et différenciation (DySAD), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.MATE]Chemical Sciences/Material chemistry, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

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