1. Extracellular vesicles of MSCs and cardiomyoblasts are vehicles for lipid mediators.
- Author
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Pizzinat N, Ong-Meang V, Bourgailh-Tortosa F, Blanzat M, Perquis L, Cussac D, Parini A, and Poinsot V
- Subjects
- Animals, Bone Marrow chemistry, Bone Marrow drug effects, Bone Marrow metabolism, Cell Line, Extracellular Vesicles drug effects, Humans, Inflammation metabolism, Inflammation Mediators chemistry, Inflammation Mediators metabolism, Lipid Metabolism, Mesenchymal Stem Cells drug effects, Myoblasts, Cardiac drug effects, Oxylipins chemistry, Oxylipins metabolism, Rats, Eicosanoids chemistry, Eicosanoids metabolism, Extracellular Vesicles chemistry, Extracellular Vesicles metabolism, Mesenchymal Stem Cells chemistry, Mesenchymal Stem Cells metabolism, Myoblasts, Cardiac chemistry, Myoblasts, Cardiac metabolism
- Abstract
Recent works reported the relevance of cellular exosomes in the evolution of different pathologies. However, most of these studies focused on the ability of exosomes to convey mi-RNA from cell to cell. The level of knowledge concerning the transport of lipid mediators by these nanovesicles is more than fragmented. The role of lipid mediators in the inflammatory signaling is fairly well described, in particular concerning the derivatives of the arachidonic acid (AA), called eicosanoïds or lipid mediators. The aim of the present work was to study the transport of these lipids within the extracellular vesicles of rat bone marrow mesenchymal stem cells (BM-MSC) and the cardiomyoblast cell line H9c2. We were able to characterize, for the first time, complete profiles of oxilipins within these nanovesicles. We studied also the impact on these profiles, of the polyunsaturated fatty acids (PUFAs) know to be precursors of the inflammatory signaling molecules (AA, eicosapentaenoic acid EPA and Docosahexaenoic acid DHA), at physiological concentrations. By growing the progenitor cells under PUFAs supplementation, we provide a comprehensive assessment of the beneficial effect of ω-3 PUFA therapy. Actually, our results tend to support the resolving role of the inflammation that stromal cell-derived extracellular vesicles can have within the cardiac microenvironment., (Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
- Published
- 2020
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