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2 results on '"Brandt, Conxita"'

1. STR analysis of artificially degraded DNA--results of a collaborative European exercise.

Author

Schneider, Peter M., Bender, Klaus, Mayr, Wolfgang R., Parson, Walther, Hoste, Bernadette, Decorte, Ronny, Cordonnier, Jan, Vanek, Daniel, Morling, Niels, Karjalainen, Matti, Carlotti, C. Marie-Paule, Sabatier, Myriam, Hohoff, Carsten, Schmitter, Hermann, Pflug, Werner, Wenzel, Rainer, Patzelt, Dieter, Lessig, Rudiger, Dobrowolski, Peter, O'Donnell, Geraldine, Garafano, Luciano, Dobosz, Marina, de Knijff, Peter, Mevag, Bente, Pawlowski, Ryszard, Gusmao, Leonor, Vide, Maria Conceicao, Alonso, Antonio, Fernandez, Oscar Garcia, Nicolas, Pilar Sanz, Kihlgreen, Ann, Bar, Walter, Meier, Verena, Teyssier, Anne, Coquoz, Raphael, Brandt, Conxita, Germann, Ursula, Gill, Peter, Hallett, Justine, and Greenhalgh, Matthew

Subjects
Polymerase chain reaction -- Analysis -- Research, Decomposition (Chemistry) -- Analysis -- Research, Allelomorphism -- Research -- Analysis
Abstract

Abstract Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. [...]

Published
2004

2. STR analysis of artificially degraded DNA-results of a collaborative European exercise

Author

Schneider, Peter M, Bender, Klaus, Mayr, Wolfgang R, Parson, Walther, Hoste, Bernadette, Decorte, Ronny, Cordonnier, Jan, Vanek, Daniel, Morling, Niels, Karjalainen, Matti, Marie-Paule Carlotti, C, Sabatier, Myriam, Hohoff, Carsten, Schmitter, Hermann, Pflug, Werner, Wenzel, Rainer, Patzelt, Dieter, Lessig, Rüdiger, Dobrowolski, Peter, O'Donnell, Geraldine, Garafano, Luciano, Dobosz, Marina, De Knijff, Peter, Mevag, Bente, Pawlowski, Ryszard, Gusmão, Leonor, Conceicao Vide, Maria, Alonso Alonso, Antonio, García Fernández, Oscar, Sanz Nicolás, Pilar, Kihlgreen, Ann, Bär, Walter, Meier, Verena, Teyssier, Anne, Coquoz, Raphael, Brandt, Conxita, Germann, Ursula, Gill, Peter, Hallett, Justine, Greenhalgh, Matthew, Schneider, Peter M, Bender, Klaus, Mayr, Wolfgang R, Parson, Walther, Hoste, Bernadette, Decorte, Ronny, Cordonnier, Jan, Vanek, Daniel, Morling, Niels, Karjalainen, Matti, Marie-Paule Carlotti, C, Sabatier, Myriam, Hohoff, Carsten, Schmitter, Hermann, Pflug, Werner, Wenzel, Rainer, Patzelt, Dieter, Lessig, Rüdiger, Dobrowolski, Peter, O'Donnell, Geraldine, Garafano, Luciano, Dobosz, Marina, De Knijff, Peter, Mevag, Bente, Pawlowski, Ryszard, Gusmão, Leonor, Conceicao Vide, Maria, Alonso Alonso, Antonio, García Fernández, Oscar, Sanz Nicolás, Pilar, Kihlgreen, Ann, Bär, Walter, Meier, Verena, Teyssier, Anne, Coquoz, Raphael, Brandt, Conxita, Germann, Ursula, Gill, Peter, Hallett, Justine, and Greenhalgh, Matthew

Abstract

Udgivelsesdato: 2004-Jan-28, Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for the analysis of degraded DNA samples. Using a batch of artificially degraded DNA with an average fragment size of approx. 200 bp a collaborative exercise was carried out among 38 forensic laboratories from 17 European countries. The results were assessed according to correct allele detection, peak height and balance as well as the occurrence of artefacts. A number of common problems were identified based on these results such as strong peak imbalance in heterozygous genotypes for the larger short tandem repeat (STR) fragments after increased PCR cycle numbers, artefact signals and allelic drop-out. Based on the observations, strategies are discussed to overcome these problems. The strategies include careful balancing of the amount of template DNA and the PCR cycle numbers, the reaction volume and the amount of Taq polymerase. Furthermore, a careful evaluation of the results of the fragment analysis and of automated allele calling is necessary to identify the correct alleles and avoid artefacts.

Published
2004

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