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134 results on '"Brecht, E."'

2. Resonators for a 2 pole filter fabricated from YBCO coated LaAlO3 cylinders

Author

Kittel, H., Klauda, M., Neumann, C., Dutzi, J., Li, Y.R., Smithey, R., Brecht, E., Schneider, R., Geerk, J., Keppler, J., and Klinger, K.

Subjects
Resonators -- Research, Electric filters, Bandpass -- Design and construction, Business, Electronics, Electronics and electrical industries
Abstract

Dielectric resonators for a two pole filter were developed and tested. They consist of LaAlO3 single crystalline cylinders covered on the cylindrical surface by 4 micrometer gold films and on the end faces by 350 nm YBa2cu3O7-delta thin films. The two-pole bandpass filters fabricated from the resonators have an insertion loss of -0.26 dB at their center frequencies of 6.3 GHz at 77 K.

Published
1997

4. The impact of HBsAg on general and HBV-specific immunity

Author

Bert, N.L., primary, Hong, M., additional, Tan, D., additional, Kunasegaran, K., additional, Cheng, Y., additional, Brecht, E., additional, Gill, U.S., additional, Frey, C., additional, Newell, E., additional, Kennedy, P., additional, and Bertoletti, A., additional

Published
2018
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10. Regulation of nif gene expression and the energetics of N 2 fixation over the diel cycle in a hot spring microbial mat

Author

Steunou, AS, Jensen, SI, Brecht, E, Becraft, ED, Bateson, MM, Kilian, O, Bhaya, D, Ward, DM, Peters, JW, Grossman, AR, and Kühl, M

Subjects
Synechococcus, Transcription, Genetic, Light, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Regulation, Bacterial, Darkness, Microbiology, Hot Springs, Oxygen Consumption, Bacterial Proteins, Nitrogen Fixation, Photosynthesis, Oxidoreductases, Energy Metabolism, Ecosystem
Abstract

Nitrogen fixation, a prokaryotic, O 2 -inhibited process that reduces N 2 gas to biomass, is of paramount importance in biogeochemical cycling of nitrogen. We analyzed the levels of nif transcripts of Synechococcus ecotypes, NifH subunit and nitrogenase activity over the diel cycle in the microbial mat of an alkaline hot spring in Yellowstone National Park. The results showed a rise in nif transcripts in the evening, with a subsequent decline over the course of the night. In contrast, immunological data demonstrated that the level of the NifH polypeptide remained stable during the night, and only declined when the mat became oxic in the morning. Nitrogenase activity was low throughout the night; however, it exhibited two peaks, a small one in the evening and a large one in the early morning, when light began to stimulate cyanobacterial photosynthetic activity, but O 2 consumption by respiration still exceeded the rate of O 2 evolution. Once the irradiance increased to the point at which the mat became oxic, the nitrogenase activity was strongly inhibited. Transcripts for proteins associated with energy-producing metabolisms in the cell also followed diel patterns, with fermentation-related transcripts accumulating at night, photosynthesis- and respiration-related transcripts accumulating during the day and late afternoon, respectively. These results are discussed with respect to the energetics and regulation of N 2 fixation in hot spring mats and factors that can markedly influence the extent of N 2 fixation over the diel cycle. © 2008 International Society for Microbial Ecology All rights reserved.

Published
2008

16. Structure and function of Parkin E3 ubiquitin ligase reveals aspects of RING and HECT ligases

Author

Riley, B.E., primary, Lougheed, J.C., additional, Callaway, K., additional, Velasquez, M., additional, Brecht, E., additional, Nguyen, L., additional, Shaler, T., additional, Walker, D., additional, Yang, Y., additional, Regnstrom, K., additional, Diep, L., additional, Zhang, Z., additional, Chiou, S., additional, Bova, M., additional, Artis, D.R., additional, Yao, N., additional, Baker, J., additional, Yednock, T., additional, and Johnston, J.A., additional

Published
2013
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20. Structure-based design of novel dihydroisoquinoline BACE-1 inhibitors that do not engage the catalytic aspartates.

Author

Bowers, B., primary, Xu, Y., additional, Yuan, S., additional, Probst, G.D., additional, Hom, R.K., additional, Chan, W., additional, Konradi, A.W., additional, Sham, H.L., additional, Zhu, Y.L., additional, Beroza, P., additional, Pan, H., additional, Brecht, E., additional, Yao, N., additional, Lougheed, J., additional, Artis, D.R., additional, Tam, D., additional, and Bova, M., additional

Published
2013
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22. In situ analysis of nitrogen fixation and metabolic switching in unicellular thermophilic cyanobacteria inhabiting hot spring microbial mats

Author

Steunou, AS, Bhaya, D, Bateson, MM, Melendrez, MC, Ward, DM, Brecht, E, Peters, JW, Kühl, M, Grossman, AR, Steunou, AS, Bhaya, D, Bateson, MM, Melendrez, MC, Ward, DM, Brecht, E, Peters, JW, Kühl, M, and Grossman, AR

Abstract

Genome sequences of two Synechococcus ecotypes inhabiting the Octopus Spring microbial mat in Yellowstone National Park revealed the presence of all genes required for nitrogenase biosynthesis. We demonstrate that nif genes of the Synechococcus ecotypes are expressed in situ in a region of the mat that varies in temperature from 53.5°C to 63.4°C (average 60°C); transcripts are only detected at the end of the day when the mat becomes anoxic. Nitrogenase activity in mat samples was also detected in the evening. Hitherto, N2 fixation in hot spring mats was attributed either to filamentous cyanobacteria (not present at >50°C in these mats) or to heterotrophic bacteria. To explore how energy-generating processes of the Synechococcus ecotypes track natural light and O2 conditions, we evaluated accumulation of transcripts encoding proteins involved in photosynthesis, respiration, and fermentation. Transcripts from photosynthesis (cpcF, cpcE, psaB, and psbB) and respiration (coxA and cydA) genes declined in the evening. In contrast, transcripts encoding enzymes that may participate in fermentation fell into two categories; some (ldh, pdhB, ald, and ackA) decreased in the evening, whereas others (pflB, pflA, adhE, and acs) increased at the end of the day and remained high into the night. Energy required for N2 fixation during the night may be derived from fermentation pathways that become prominent as the mat becomes anoxic. In a broader context, our data suggest that there are critical regulatory switches in situ that are linked to the diel cycle and that these switches alter many metabolic processes within the microbial mat. © 2006 by The National Academy of Sciences of the USA.

Published
2006

41. Orientational Changes in the ab plane of YBA2Cu3O7−δ Films on Different Substrates

Author

Steinborn, T., primary, Adrian, H., additional, Brecht, E., additional, Fuess, H., additional, Maul, M., additional, Miehe, G., additional, Petersen, K., additional, Rodewald, M., additional, Rao, M., additional, Schmahl, W. W., additional, Traeholt, C., additional, Wiesner, J., additional, Wirth, G., additional, Zandbergen, H., additional, and Zegenhagen, J., additional

Published
1996
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