1. A Type III Polyketide Synthase Specific for Sporulating Negativicutes is Responsible for Alkylpyrone Biosynthesis.
- Author
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Bredy F, Ishida K, and Hertweck C
- Subjects
- Bacteria, Anaerobic metabolism, Escherichia coli genetics, Escherichia coli metabolism, Gram-Negative Bacteria metabolism, Firmicutes, Polyketide Synthases genetics, Polyketide Synthases metabolism, Polyketides metabolism
- Abstract
Genomic analyses indicate that anaerobic bacteria represent a neglected source of natural products. Whereas a limited number of polyketides have been reported from anaerobes, products of type III polyketide synthases (PKSs) have remained unknown. We found a highly conserved biosynthetic gene cluster (BGC) comprising genes putatively encoding a type III PKS and a methyltransferase in genomes of the Negativicutes, strictly anaerobic, diderm bacteria. By in vivo and in vitro expression of a type III PKS gene, dquA from the oak-associated Dendrosporobacter quercicolus in E. coli we show production of long-chain alkylpyrones. Intriguingly, this BGC is specific for sporulating Sporomusaceae but absent in related Negativicutes that do not sporulate, thus suggesting a physiological role., (© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.)
- Published
- 2022
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