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Identifying immune correlates of protection is a major challenge in AIDS vaccine development. Anti-Envelope antibodies have been considered critical for protection against SIV/HIV (SHIV) acquisition. Here, we evaluated the efficacy of an SHIV vaccine against SIVmac251 challenge, where the role of antibody was excluded, as there was no cross-reactivity between SIV and SHIV envelope antibodies. After 8 low-dose intrarectal challenges with SIVmac251, 12 SHIV-vaccinated animals demonstrated efficacy, compared with 6 naive controls, suggesting protection was achieved in the absence of anti-envelope antibodies. Interestingly, CD8+ T cells (and some NK cells) were not essential for preventing viral acquisition, as none of the CD8-depleted macaques were infected by SIVmac251 challenges. Initial investigation of protective innate immunity revealed that protected animals had elevated pathways related to platelet aggregation/activation and reduced pathways related to interferon and responses to virus. Moreover, higher expression of platelet factor 4 on circulating platelet-leukocyte aggregates was associated with reduced viral acquisition. Our data highlighted the importance of innate immunity, identified mechanisms, and may provide opportunities for novel HIV vaccines or therapeutic strategy development.

Australia is a sink for low pathogenicity avian influenza viruses, with isolated circulation occurring on the continent. We report the incursion of a Eurasian low pathogenicity avian influenza H5 virus into Australia. This report benefits surveillance and diagnostic work because of the risk and current absence of highly pathogenic avian influenza A(H5N1).

Abstract A critical component of ecosystem restoration projects involves using genetic data to select source material that will enhance success under current and future climates. However, the complexity and expense of applying genetic data is a barrier to its use outside of specialised scientific contexts. To help overcome this barrier, we developed Reef Adapt ( www.reefadapt.org ), an innovative, globally applicable and expandable web platform that incorporates genetic, biophysical and environmental prediction data into marine restoration and assisted gene flow planning. The Reef Adapt tool provides maps that identify areas with populations suited to user-specified restoration/recipient sites under current and future climate scenarios. We demonstrate its versatility and practicality with four case studies of ecologically and evolutionarily diverse taxa: the habitat-forming corals Pocillopora damicornis and Acropora kenti, and macroalgae Phyllospora comosa and Ecklonia radiata. Reef Adapt is a management-ready tool to aid restoration and conservation efforts amidst ongoing habitat degradation and climate change.

Terrestrial invertebrates in urban ecosystems are extremely species-rich, have many important roles in material flow and energy circulation, and are host to many human pathogens that pose threats to human health. These invertebrates are widely distributed in urban areas, including both out- and in-door environments. Consequently, humans are frequently in contact with them, which provides many opportunities for them to pose human health risks. However, comprehensive knowledge on human pathogen transfer via invertebrates is lacking, with research to date primarily focused on dipterans (e.g., mosquitoes, flies). Here, we take a broad taxonomic approach and review terrestrial invertebrate hosts (incl. mosquitoes, flies, termites, cockroaches, mites, ticks, earthworms, collembola, fleas, snails, and beetles) of human pathogens, with a focus on transmission pathways. We also discuss how urbanization and global warming are likely to influence the communities of invertebrate hosts and have flow-on risks to human health. Finally, we identify current research gaps and provide perspectives on future directions.

Cryoelectron microscopy (Cryo-EM) has enabled structural determination of proteins larger than about 50 kDa, including many intractable by any other method, but it has largely failed for smaller proteins. Here, we obtain structures of small proteins by binding them to a rigid molecular scaffold based on a designed protein cage, revealing atomic details at resolutions reaching 2.9 Å. We apply this system to the key cancer signaling protein KRAS (19 kDa in size), obtaining four structures of oncogenic mutational variants by cryo-EM. Importantly, a structure for the key G12C mutant bound to an inhibitor drug (AMG510) reveals significant conformational differences compared to prior data in the crystalline state. The findings highlight the promise of cryo-EM scaffolds for advancing the design of drug molecules against small therapeutic protein targets in cancer and other human diseases.

Abstract The interrelationship between climate change, pollution and the aerobiome (the microbiome of the air) is a complex ecological dynamic with profound implications for human and ecosystem health. This mini‐review explores the multifaceted relationships among these factors. By synthesising existing research and integrating interdisciplinary perspectives, we examine the mechanisms driving interactions within the climate change–pollution–aerobiome nexus. We also explore synergistic and cascading effects and potential impacts on human health (including both communicable and non‐communicable diseases) and that of wider ecosystems. Based on our mini‐review results, climate change influences air pollution and, independently, air pollution affects the composition, diversity and activity of the aerobiome. However, we apply a ‘systems thinking’ approach and create a set of systems diagrams to show that climate change likely influences the aerobiome (including bacteria and fungi) via climate change–pollution interactions in complex ways. Due to the inherent complexity of these systems, we emphasise the importance of holistic and/or interdisciplinary approaches and collaborative efforts in understanding this nexus to safeguard planetary health in an era of rapid environmental change.

There have been limited efforts in using collective memory in representing public spaces and implementing urban renewal projects to maintain uniqueness. This article aims to investigate the role of urban landscape in shaping memories and proposes recommendations that reinforce urban landscape identity. This article postulates that a city’s individual and collective memory is important in maintaining its uniqueness in terms of urban landscape identity. A qualitative research design was adopted. Photo elicitation interviews (PEI) were used to investigate the features that triggered memory of the participants and were important in contributing to the image of Kisumu City. There were ongoing and completed urban renewal projects within Kisumu City, Kenya at the time of the study. 12 PEI participants were purposively selected in addition to four officials from the County Government as key informants and four professionals were interviewed. Field investigation by the researcher was carried out through mapping and photography. The study revealed that the old railway station as human-made element was instrumental in memory through historical development while socio-cultural activities at Kisumu Sports Grounds and social interaction at public Central Square were also highly regarded in memory development. Despite the important interplay of natural, human-made and social interactions in the formation of memory, place and identity, there was also a very positive response from residents on the new look of the city. The physical upgrade and cleaning of the city and its public spaces, which also means greater safety, was well appreciated from a socio-economic point of view.

Abstract Arts-based research offers an innovative approach to today’s social-ecological challenges. Many authors ascertain the transformative power of arts-based research lies in its ability to generate holistic and collaborative encounters which facilitate change. To advance critical discussions around arts-based research, the following article opens up pockets of success as well as the limitations and failures of the My Mabopane photovoice project. This project intended to support urban transformation in the City of Tshwane, South Africa, by asking participants to photograph and reflect upon a nearby green space afflicted with various social and environmental challenges. Results suggest alternative ways of knowing, which contribute to transformative learning, can link arts-based research to social-ecological transformation. However, despite this promising pathway, analyses also reveal realizing social-ecological transformation with arts-based research is no walk in the park. Rather problematic assumptions around knowledge and knowledge production, emerging in tensions between art and science, often trump the emancipatory intentions of photovoice. And broader structural challenges, that arts-based research situates itself in and against, pose a strong barrier for social-ecological transformation. In response to these barriers, this article argues for a collaborative arts-based research practice centering community realities on multiple scales via a merging of art, science, and life.

Introduction: Food insecurity (FI) has been associated with adverse health outcomes and increased healthcare expenditures. Many families experienced reduced access to food during the coronavirus disease 2019 (COVID-19) pandemic. A 2019 study revealed that the pre-pandemic prevalence of FI at an urban, tertiary care hospital’s emergency department (ED) was 35.3%. We sought to evaluate whether the prevalence of FI in the same ED patient population increased during the COVID-19 pandemic. Methods: We performed a single-center, observational, survey-based study. Surveys assessing for FI were administered to clinically stable patients presenting to the ED over 25 consecutive weekdays from November–December 2020. Results: Of 777 eligible patients, 379 (48.8%) were enrolled; 158 (41.7%) screened positive for FI. During the pandemic, there was a 18.1% relative increase (or 6.4% absolute increase) in the prevalence of FI in this population (P=0.040; OR=1.309, 95% CI 1.012-1.693). The majority (52.9%) of food-insecure subjects reported reduced access to food due to the pandemic. The most common perceived barriers to access to food were reduced food availability at grocery stores (31%), social distancing guidelines (26.5%), and reduced income (19.6%).  Conclusion: Our findings suggest that nearly half of the clinically stable patients who presented to our urban ED during the pandemic experienced food insecurity. The prevalence of FI in our hospital’s ED patient population increased by 6.4% during the pandemic. Emergency physicians should be aware of rising FI in their patient population so that they may better support patients who must choose between purchasing food and purchasing prescribed medications.

Background: The use of virtual reality (VR) in medical education allows learners to make mistakes safely without risk to patients and to refine a range of clinical skills by repetition. However, there is still wide variability both between, and within countries regarding the amount of training delivered using VR, particularly in relation to intensive care medicine Objectives: To identify the range of uses, phase of development and effectiveness of VR for intensive care unit (ICU) staff training. Methods: The review followed the scoping review framework set out by Levac et al. (2010). A multi-database search was undertaken. All study types were included if they explored the use of VR for intensive care staff (ICU) staff training. Full paper screening, data extraction and assessment of bias was carried out by a single reviewer with verification by a second reviewer. A narrative synthesis was chosen to summarize the data. Results: The search strategy identified 647 records. Following the removal of duplicates, screening of titles, abstracts and full texts, five studies were included. VR for ICU training has primarily focused on skill acquisition for surgical procedures. The majority of studies in this area were classified to be at an early stage of assessing acceptability, tolerability and efficacy. There was very low-quality evidence that VR for ICU training is effective. Conclusions: Studies have explored the use of VR for a small number of surgical procedures and emergency patient scenarios. VR for ICU training demonstrates some potential, however further development and high-quality research is required. Research relating to virtual reality for ICU staff training is currently of weak methodological quality and as such, no recommendations to clinical practise can be made. Wherever possible when using VR for ICU staff training it should be utilized as part of a high-quality study.

Lumpy skin disease (LSD) is an infectious disease currently spreading worldwide and poses a serious global threat. However, there is limited evidence and understanding to support the use of models to inform decision-making in LSD outbreak responses. This review aimed to identify modelling approaches that can be used before and during an outbreak of LSD, examining their characteristics and priorities, and proposing a structured workflow. We conducted a systematic review and identified 60 relevant publications on LSD outbreak modelling. The review identified six categories of question to be addressed following outbreak detection (origin, entry pathway, outbreak severity, risk factors, spread, and effectiveness of control measures), and five analytical techniques used to address them (descriptive epidemiology, risk factor analysis, spatiotemporal analysis, dynamic transmission modelling, and simulation modelling). We evaluated the questions each analytical technique can address, along with their data requirements and limitations, and accordingly assigned priorities to the modelling. Based on this, we propose a structured workflow for modelling during an LSD outbreak. Additionally, we emphasise the importance of pre-outbreak preparation and continuous updating of modelling post-outbreak for effective decision-making. This study also discusses the inherent limitations and uncertainties in the identified modelling approaches. To support this workflow, high-quality data must be collected in standardised formats, and efforts should be made to reduce inherent uncertainties of the models. The suggested modelling workflow can be used as a process to support rapid response for countries facing their first LSD occurrence and can be adapted to other transboundary diseases.

Abstract Soil microbiota underpin ecosystem functionality yet are rarely targeted during ecosystem restoration. Soil microbiota recovery following native plant revegetation can take years to decades, while the effectiveness of soil inoculation treatments on microbiomes remains poorly explored. Therefore, innovative restoration treatments that target soil microbiota represent an opportunity to accelerate restoration outcomes. Here, we introduce the concept of ecological phage therapy—the application of phage for the targeted reduction of the most abundant and dominant bacterial taxa present in degraded ecosystems. We propose that naturally occurring bacteriophages—viruses that infect bacteria—could help rapidly shift soil microbiota towards target communities. Bacteriophages sculpt the microbiome by lysis of specific bacteria, and if followed by the addition of reference soil microbiota, such treatments could facilitate rapid reshaping of soil microbiota. Here, we experimentally tested this concept in a pilot study. We collected five replicate pre‐treatment degraded soil samples, then three replicate soil samples 48 hours after phage, bacteria, and control treatments. Bacterial 16S rDNA sequencing showed that phage‐treated soils had reduced bacterial diversity; however, when we combined ecological phage therapy with reference soil inoculation, we did not see a shift in soil bacterial community composition from degraded soil towards a reference‐like community. Our pilot study provides early evidence that ecological phage therapy could help accelerate the reshaping of soil microbiota with the ultimate aim of reducing timeframes for ecosystem recovery. We recommend the next steps for ecological phage therapy be (a) developing appropriate risk assessment and management frameworks, and (b) focussing research effort on its practical application to maximise its accessibility to restoration practitioners.

Abstract Environmental change exerts a profound effect on soil microbial domains—including bacteria, fungi, and protists—that each perform vital ecological processes. While these microbial domains are ubiquitous and extremely diverse, little is known about how they respond to environmental changes in urban soil ecosystems and what ecological processes shape them. Here we investigated the community assembly processes governing bacteria, fungi, and protists through the lens of four distinct subcommunities: abundant, conditionally rare, conditionally abundant, and rare taxa. We show that transient taxa, including the conditionally rare and conditionally rare or abundant taxa, were the predominant subcommunities. Deterministic processes (e.g., environmental filtering) had major roles in structuring all subcommunities of fungi, as well as conditionally rare and abundant protists. Stochastic processes had strong effects in structuring all subcommunities of bacteria (except rare taxa) and conditionally rare protists. Overall, our study underscores the importance of complementing the traditional taxonomy of microbial domains with the subcommunity approach when investigating microbial communities in urban soil ecosystems.

Abstract Butyrate‐producing bacteria colonise the gut of humans and non‐human animals, where they produce butyrate, a short‐chain fatty acid with known health benefits. Butyrate‐producing bacteria also reside in soils and soil bacteria can drive the assembly of airborne bacterial communities (the aerobiome). Aerobiomes in urban greenspaces are important reservoirs of butyrate‐producing bacteria as they supplement the human microbiome, but soil butyrate producer communities have rarely been examined in detail. Here, we studied soil metagenome taxonomic and functional profiles and soil physicochemical data from two urban greenspace types: sports fields (n = 11) and nature parks (n = 22). We also developed a novel method to quantify soil butyrate and characterised the in situ activity of butyrate‐producing bacteria. We show that soil butyrate was higher in sports fields than nature parks and that sports fields also had significantly higher relative abundances of the terminal butyrate production genes buk and butCoAT than nature parks. Soil butyrate positively correlated with buk gene abundance (but not butCoAT). Soil moisture (r = .50), calcium (r = −.62), iron (ρ = .54), ammonium nitrogen (ρ = .58) and organic carbon (r = .45) had the strongest soil abiotic effects on soil butyrate concentrations and iron (ρ = .56) and calcium (ρ = −.57) had the strongest soil abiotic effects on buk read abundances. Overall, our findings contribute important new insights into the role of sports fields as key exposure reservoirs of butyrate producing bacteria, with important implications for the provision of microbiome‐mediated human health benefits via butyrate.

Warming sea surface temperatures (SSTs) are altering the biological structure of intertidal wetlands at a global scale, with potentially serious physiological and demographic consequences for migratory shorebird populations that depend on intertidal sites. The effects of mediating factors, such as age-related foraging skill, in shaping the consequences of warming SSTs on shorebird populations, however, remain largely unknown. Using morphological measurements of Dunlin fuelling for a >3000 km transoceanic migration, we assessed the influence of climatic conditions and age on individuals’ migratory fuel loads and performance. We found that juveniles were often at risk of exhausting their fuel loads en route to primary wintering grounds, especially following high June SSTs in the previous year; the lagged nature of which suggests SSTs acted on juvenile loads by altering the availability of critical prey. Up to 45% fewer juveniles may have reached wintering grounds via a non-stop flight under recent high SSTs compared to the long-term trend. Adults, by contrast, were highly capable of reaching wintering grounds in non-stop flight across years. Our findings suggest that juveniles were disproportionately impacted by apparent SST-related declines in critical prey, and illustrate a general mechanism by which climate change may shape migratory shorebird populations worldwide.

This article explores how sharing and listening to stories linked children and young people, educators, artists, civil society workers, and policymakers as part of a continuum of transitional justice processes in the aftermath of conflict Mobile Arts for Peace (MAP) initiative. We argue that sharing stories within a peace education context can potentially help to address everyday conflicts and personal and social wounds, often due to the residue of past conflicts. MAP facilitates a community of listening and/or a community of listeners through arts-based approaches that engages the individual to listen to oneself, the community to listen to the other, and the society to listen to inform everyday peacebuilding.

Scripture’s reference to believers as the ‘family of God’ may indicate that believers should mutually care for one another like members of a caring family do, and not merely that members are part of a ‘spiritual’ family. From this perspective, the article investigated the extent of mutual care within congregations. The increasing need for pastoral care, within and outside church congregations, reveals the need for mutual care between members. The necessity for empirical studies within church congregations were revealed by the differences in the intensity of the answers by the participants. Without knowledge about the experiences of church members, leaders of church congregations may overlook potential needs of their members. A quantitative empirical study was done through an anonymous online survey with church members, pastoral counsellors, and pastors and ministers from various denominations who completed the survey, according to their experiences within their individual church congregation. The concepts of fellowship (koinōnia), service (diakonia), care, and family were used in the survey to determine the experiences of mutual care between members of the church congregation. The deficiency of mutual care between members of the congregation may motivate leaders to intentionally develop relationships through fellowship (koinōnia) to foster mutual care between members, and intentionally develop members to serve (diakonia) one another and their community. Contribution: This article contributes to an interdisciplinary discussion between pastoral theology, practical theology, and systematic theology from a Reformed perspective with a focus on the practical participation of members of the church congregation in pastoral care as the ‘family of God’.

Abstract Butyrate‐producing bacteria are found in many outdoor ecosystems and host organisms, including humans, and are vital to ecosystem functionality and human health. These bacteria ferment organic matter, producing the short‐chain fatty acid butyrate. However, the macroecological influences on their biogeographical distribution remain poorly resolved. Here we aimed to characterise their global distribution together with key explanatory climatic, geographical and physicochemical variables. We developed new normalised butyrate production capacity (BPC) indices derived from global metagenomic (n = 13,078) and Australia‐wide soil 16S rRNA (n = 1331) data, using Geographic Information System (GIS) and modelling techniques to detail their ecological and biogeographical associations. The highest median BPC scores were found in anoxic and fermentative environments, including the human (BPC = 2.99) and non‐human animal gut (BPC = 2.91), and in some plant–soil systems (BPC = 2.33). Within plant–soil systems, roots (BPC = 2.50) and rhizospheres (BPC = 2.34) had the highest median BPC scores. Among soil samples, geographical and climatic variables had the strongest overall effects on BPC scores (variable importance score range = 0.30–0.03), with human population density also making a notable contribution (variable importance score = 0.20). Higher BPC scores were in soils from seasonally productive sandy rangelands, temperate rural residential areas and sites with moderate‐to‐high soil iron concentrations. Abundances of butyrate‐producing bacteria in outdoor soils followed complex ecological patterns influenced by geography, climate, soil chemistry and hydrological fluctuations. These new macroecological insights further our understanding of the ecological patterns of outdoor butyrate‐producing bacteria, with implications for emerging microbially focused ecological and human health policies.

Leading and equipping people for good deeds as a crucial part of parish ministry. The article examines the role that equipment for good works, as an integral part of church ministry, can play in revitalising and help congregations to grow. The investigation is conducted through an exegetical analysis of Ephesians 2, focusing on verse 10. The results of the study of Ephesians are then compared with other Scriptures where good works are mentioned. From this research, it becomes clear that good works do not play a role in the justification and salvation of believers, because it is solely the work of God’s undeserved grace. However, good works are an integral part of God’s purpose in his redemption of humanity. Humanity is not saved by good works but rather unto good works. According to Ephesians, good works serve to proclaim the greatness of God’s grace towards believers. God prepares the good works for believers. Believers are enabled to do good works through the gifts that Christ and the Spirit give them, and the equipment they receive through the special services. From the Gospels, it is evident that good works were a characteristic of Jesus’ mission, and therefore also the calling of his disciples. In the pastoral letters, good works are described as part of Christ’s purpose for which He acquired a people for Himself. The ultimate goal of equipping from the Word, is the ability to do good works. Therefore, good works testify to maturity in faith, reliability, and dedication. A lack of good works puts a question mark on someone’s faith. The conclusion from the research is that deliberate equipping of members to actively do good works, should be an integral part of church ministry. Each member of the congregation should be held responsible for using the opportunities that God creates for them, to do good works. The lack of a structured ministry of equipping members for distributing God’s grace by good works, can be a leading cause of stagnation and decline in congregations. Contribution: The article contributes to practical theological reflection on ministry in the church through in-depth exegetical analysis and comparison of Scripture with Scripture. The place of equipping for good works, which is often overlooked in reformed circles, is highlighted here.

Urban renewal is undertaken to respond to the physical deterioration of built forms in postcolonial Africa, with renewal works affecting current cities’ identities. Globalisation trends have cities striving to be unique, with a growing awareness of the importance of identity. Landscape identity is adopted in this study as the overall term that includes other identities and is interpreted as residents’ perception of the special features that help them differentiate between places. This study postulates that a city’s uniqueness lies in its landscape identity and that this should not be neglected. The article investigates the concept of landscape identity in the context of a case study of the city of Kisumu, Kenya, which has recently undergone urban renewal. The aim of the study was to identify the main aspects that constitute the formation of landscape identity in Kisumu. A mixed-methods approach was used, which included field investigations, a survey with 384 participants, and four key informant interviews. The survey results revealed that the city’s location along Lake Victoria, which represented an element of the natural environment, gave it the highest rating. The proximity to Lake Victoria and views of the hills were regarded as the most outstanding features of the city, while the lake was the highest ranked element with symbolic meaning that evoked individual and collective memories. These findings suggest that the urban landscape identity in Kisumu is strongly connected to features of the natural environment.

Abstract Step selection analysis (SSA) is a common framework for understanding animal movement and resource selection using telemetry data. Such data are, however, inherently autocorrelated in space, a complication that could impact SSA‐based inference if left unaddressed. Accounting for spatial correlation is standard statistical practice when analysing spatial data, and its importance is increasingly recognized in ecological models (e.g. species distribution models). Nonetheless, no framework yet exists to account for such correlation when analysing animal movement using SSA. Here, we extend the popular method integrated step selection analysis (iSSA) by including a Gaussian field (GF) in the linear predictor to account for spatial correlation. For this, we use the Bayesian framework R‐INLA and the stochastic partial differential equations (SPDE) technique. We show through a simulation study that our method provides accurate fixed effects estimates, quantifies their uncertainty well and improves the predictions. In addition, we demonstrate the practical utility of our method by applying it to three wolverine (Gulo gulo) tracks. Our method solves the problems of assuming spatially independent residuals in the SSA framework. In addition, it offers new possibilities for making long‐term predictions of habitat usage.