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1. Xenotransplantation von Organen

Author

Schmoeckel, Michael, Längin, Matthias, Reichart, Bruno, Abicht, Jan-Michael, Bender, Martin, Denner, Joachim, Marckmann, Georg, Brenner, Paolo, Wolf, Eckhard, and Hagl, Christian

Published
2024
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3. Xenografts Show Signs of Concentric Hypertrophy and Dynamic Left Ventricular Outflow Tract Obstruction After Orthotopic Pig-to-baboon Heart Transplantation

Author

Längin, Matthias, Buttgereit, Ines, Reichart, Bruno, Panelli, Alessandro, Radan, Julia, Mokelke, Maren, Neumann, Elisabeth, Bender, Martin, Michel, Sebastian, Ellgass, Reinhard, Ying, Jiawei, Fresch, Ann Kathrin, Mayr, Tanja, Steen, Stig, Paskevicius, Audrius, Egerer, Stefanie, Bähr, Andrea, Kessler, Barbara, Klymiuk, Nikolai, Binder, Uli, Skerra, Arne, Ledderose, Stephan, Müller, Susanna, Walz, Christoph, Hagl, Christian, Wolf, Eckhard, Ayares, David, Brenner, Paolo, and Abicht, Jan-Michael

Published
2023
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7. 414.7: The Perioperative Cardiac Xenograft Dysfunction (PCXD) Has A Major Impact in (Life-Supporting) Orthotopic (oXTx) Cardiac Xenotransplantation, but Not in the Heterotopic Thoracic (htXTx) Xenotransplantation

Author

Brenner, Paolo, Reichart, Bruno, Längin, Matthias, Bender, Martin, Mayr, Tanja, Güthoff, Sonja, Michel, Sebastian, Buchholz, Stefan, Radan, Julia, Mokelke, Maren, Buttgereit, Ines, Neumann, Elisabeth, Bauer, Andreas, Klymiuk, Nikolai, Wolf, Eckhard, Walz, Christoph, Reimann, Keith, Ayares, David, Hagl, Christian, Steen, Stig, and Abicht, Jan-Michael

Published
2022
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8. Current Status of Cardiac Xenotransplantation: Report of a Workshop of the German Heart Transplant Centers, Martinsried, March 3, 2023.

Author

Schmoeckel, Michael, Längin, Matthias, Reichart, Bruno, Abicht, Jan-Michael, Bender, Martin, Michel, Sebastian, Kamla, Christine-Elena, Denner, Joachim, Tönjes, Ralf Reinhard, Schwinzer, Reinhard, Marckmann, Georg, Wolf, Eckhard, Brenner, Paolo, and Hagl, Christian

Subjects
HEART transplantation, XENOTRANSPLANTATION, CLINICAL medicine, COMPETENT authority, CARDIAC patients
Abstract

This report comprises the contents of the presentations and following discussions of a workshop of the German Heart Transplant Centers in Martinsried, Germany on cardiac xenotransplantation. The production and current availability of genetically modified donor pigs, preservation techniques during organ harvesting, and immunosuppressive regimens in the recipient are described. Selection criteria for suitable patients and possible solutions to the problem of overgrowth of the xenotransplant are discussed. Obviously microbiological safety for the recipient and close contacts is essential, and ethical considerations to gain public acceptance for clinical applications are addressed. The first clinical trial will be regulated and supervised by the Paul-Ehrlich-Institute as the National Competent Authority for Germany, and the German Heart Transplant Centers agreed to cooperatively select the first patients for cardiac xenotransplantation. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Transthoracic echocardiography is a simple tool for size matching in cardiac xenotransplantation.

Author

Mokelke, Maren, Bender, Martin, Reichart, Bruno, Neumann, Elisabeth, Radan, Julia, Buttgereit, Ines, Ayares, David, Wolf, Eckhard, Brenner, Paolo, Abicht, Jan‐Michael, and Längin, Matthias

Subjects
HEART size, XENOTRANSPLANTATION, ECHOCARDIOGRAPHY, HEART transplantation, BODY weight
Abstract

Background: Preoperative size matching is essential for both allogeneic and xenogeneic heart transplantation. In preclinical pig‐to‐baboon xenotransplantation experiments, porcine donor organs are usually matched to recipients by using indirect parameters, such as age and total body weight. For clinical use of xenotransplantation, a more precise method of size measurement would be desirable to guarantee a "perfect match." Here, we investigated the use of transthoracic echocardiography (TTE) and described a new method to estimate organ size prior to xenotransplantation. Methods: Hearts from n = 17 genetically modified piglets were analyzed by TTE and total heart weight (THW) was measured prior to xenotransplantation into baboons between March 2018 and April 2022. Left ventricular (LV) mass was calculated according to the previously published method by Devereux et al. and a newly adapted formula. Hearts from n = 5 sibling piglets served as controls for the determination of relative LV and right ventricular (RV) mass. After explantation, THW and LV and RV mass were measured. Results: THW correlated significantly with donor age and total body weight. The strongest correlation was found between THW and LV mass calculated by TTE. Compared to necropsy data of the control piglets, the Devereux formula underestimated both absolute and relative LV mass, whereas the adapted formula yielded better results. Combining the adapted formula and the relative LV mass data, THW can be predicted with TTE. Conclusions: We demonstrate reliable LV mass estimation by TTE for size matching prior to xenotransplantation. An adapted formula provides more accurate results of LV mass estimation than the generally used Devereux formula in the xenotransplantation setting. TTE measurement of LV mass is superior for the prediction of porcine heart sizes compared to conventional parameters such as age and total body weight. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Pig-to-non-human primate heart transplantation: The final step toward clinical xenotransplantation?

Author

Reichart, Bruno, Längin, Matthias, Radan, Julia, Mokelke, Maren, Buttgereit, Ines, Ying, Jiawei, Fresch, Ann Kathrin, Mayr, Tanja, Issl, Lara, Buchholz, Stefan, Michel, Sebastian, Ellgass, Reinhard, Mihalj, Maks, Egerer, Stefanie, Baehr, Andrea, Kessler, Barbara, Kemter, Elisabeth, Kurome, Mayuko, Zakhartchenko, Valeri, Steen, Stig, Sjöberg, Trygve, Paskevicius, Audrius, Krüger, Luise, Fiebig, Uwe, Denner, Joachim, Godehardt, Antonia W., Tönjes, Ralf R., Milusev, Anastasia, Rieben, Robert, Sfriso, Riccardo, Walz, Christoph, Kirchner, Thomas, Ayares, David, Lampe, Karen, Schönmann, Uwe, Hagl, Christian, Wolf, Eckhard, Klymiuk, Nikolai, Abicht, Jan-Michael, and Brenner, Paolo

Published
2020
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11. Cardiac xenotransplantation in Germany

Author

Kamla, Christine-Elena, primary, Längin, Matthias, additional, Reichart, Bruno, additional, Wolf, Eckhard, additional, Denner, Joachim, additional, Abicht, Jan-Michael, additional, Marckmann, Georg, additional, Brenner, Paolo, additional, Blesse, Stefan, additional, Michel, Sebastian, additional, and Hagl, Christian, additional

Published
2023
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12. Consistent success in life-supporting porcine cardiac xenotransplantation

Author

Längin, Matthias, Mayr, Tanja, Reichart, Bruno, Michel, Sebastian, Buchholz, Stefan, Guethoff, Sonja, Dashkevich, Alexey, Baehr, Andrea, Egerer, Stefanie, Bauer, Andreas, Mihalj, Maks, Panelli, Alessandro, Issl, Lara, Ying, Jiawei, Fresch, Ann Kathrin, Buttgereit, Ines, Mokelke, Maren, Radan, Julia, Werner, Fabian, Lutzmann, Isabelle, Steen, Stig, Sjöberg, Trygve, Paskevicius, Audrius, Qiuming, Liao, Sfriso, Riccardo, Rieben, Robert, Dahlhoff, Maik, Kessler, Barbara, Kemter, Elisabeth, Kurome, Mayuko, Zakhartchenko, Valeri, Klett, Katharina, Hinkel, Rabea, Kupatt, Christian, Falkenau, Almuth, Reu, Simone, Ellgass, Reinhard, Herzog, Rudolf, Binder, Uli, Wich, Günter, Skerra, Arne, Ayares, David, Kind, Alexander, Schönmann, Uwe, Kaup, Franz-Josef, Hagl, Christian, Wolf, Eckhard, Klymiuk, Nikolai, Brenner, Paolo, and Abicht, Jan-Michael

Published
2018
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15. Hemodynamics in pig‐to‐baboon heterotopic thoracic cardiac xenotransplantation: Recovery from perioperative cardiac xenograft dysfunction and impairment by cardiac overgrowth.

Author

Bender, Martin, Panelli, Alessandro, Reichart, Bruno, Radan, Julia, Mokelke, Maren, Neumann, Elisabeth, Buttgereit, Ines, Michel, Sebastian, Bauer, Andreas, Fresch, Ann Kathrin, Mayr, Tanja, Werner, Fabian, Egerer, Stefanie, Bähr, Andrea, Kessler, Barbara, Klymiuk, Nikolai, Ayares, David, Wolf, Eckhard, Hagl, Christian, and Brenner, Paolo

Subjects
HEART diseases, XENOTRANSPLANTATION, HEMODYNAMICS, PRESERVATION of organs, tissues, etc., CARDIAC output
Abstract

Introduction: Orthotopic cardiac xenotransplantation has seen notable improvement, leading to the first compassionate use in 2022. However, it remains challenging to define the clinical application of cardiac xenotransplantation, including the back‐up strategy in case of xenograft failure. In this regard, the heterotopic thoracic technique could be an alternative to the orthotopic procedure. We present hemodynamic data of heterotopic thoracic pig‐to‐baboon transplantation experiments, focusing on perioperative xenograft dysfunction and xenograft overgrowth. Methods: We used 17 genetically modified piglets as donors for heterotopic thoracic xenogeneic cardiac transplantation into captive‐bred baboons. In all animals, pressure probes were implanted in the graft's left ventricle and the recipient's ascending aorta and hemodynamic data (graft pressure, aortic pressure and recipient's heart rate) were recorded continuously. Results: Aortic pressures and heart rates of the recipients' hearts were postoperatively stable in all experiments. After reperfusion, three grafts presented with low left ventricular pressure indicating perioperative cardiac dysfunction (PCXD). These animals recovered from PCXD within 48 h under support of the recipient's heart and there was no difference in survival compared to the other 14 ones. After 48 h, graft pressure increased up to 200 mmHg in all 17 animals with two different time‐patterns. This led to a progressive gradient between graft and aortic pressure. With increasing gradient, the grafts stopped contributing to cardiac output. Grafts showed a marked weight increase from implantation to explantation. Conclusion: The heterotopic thoracic cardiac xenotransplantation technique is a possible method to overcome PCXD in early clinical trials and an experimental tool to get a better understanding of PCXD. The peculiar hemodynamic situation of increasing graft pressure but missing graft's output indicates outflow tract obstruction due to cardiac overgrowth. The heterotopic thoracic technique should be successful when using current strategies of immunosuppression, organ preservation and donor pigs with smaller body and organ size. [ABSTRACT FROM AUTHOR]

Published
2024
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16. WORLDWIDE FIRST FINALIZED STUDY OF PRECLINICAL LIFE-SUPPORTING ORTHOTOPIC PIG-TO-BABOON CARDIAC XENOTRANSPLANTATION (XT): CONSTANT REPRODUCIBLE 3-MONTHS-SURVIVAL UP TO HALF A YEAR MEETS THE ISHLT GUIDELINES FOR FIRST CLINICAL TRIALS

Author

Brenner, Paolo, Reichart, Bruno, Längin, Matthias, Mayr, Tanja, Guethoff, Sonja, Buchholz, Stefan, Michel, Sebastian, Lutzmann, Isabelle, Werner, Fabian, Issl, Lara, Ying, Jiawei, Fresch, Ann Kathrin, Mokelke, Maren, Radan, Julia, Buttgereit, Ines, Bauer, Andreas, Klymiuk, Nikolai, Hermanns, Walter, Reimann, Keith, Ayares, David, Hagl, Christian, Steen, Stig, Wolf, Eckhard, and Abicht, Jan-Michael

Published
2020
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18. Study for Comparison of CD40-mAb and CD40L-Ab Costimulation Blockade after Life-Supporting Orthotopic Cardiac Xenotransplantation of Multi-Transgenic Pig Hearts into Baboons with a Worldwide First Successful Long-Term Survival

Author

Brenner, Paolo, Reichart, Bruno, Mayr, Tanja, Längin, Matthias, Güthoff, Sonja, Buchholz, Stefan, Michel, Sebastian, Lutzmann, Isabelle, Werner, Fabian, Bauer, Andreas, Klymiuk, Nikolai, Wolf, Eckhard, Reimann, Keith, Hermanns, Walter, Ayares, David, Hagl, Christian, Steen, Stig, and Abicht, Jan-Michael

Published
2018
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19. Overcoming perioperative inflammation as a hurdle for successful preclinical orthotopic cardiac xenogeneic transplantations – particular in regard of the mandatory use of heart‐lung machines

Author

Bender, Martin, primary, Längin, Matthias, additional, Reichart, Bruno, additional, Mokelke, Maren, additional, Radan, Julia, additional, Neumann, Elisabeth, additional, Michel, Sebastian, additional, Ellgass, Reinhard, additional, Cowan, Peter J., additional, Wolf, Eckhard, additional, Abicht, Jan‐Michael, additional, and Brenner, Paolo, additional

Published
2022
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20. Long-Term Follow-Up of Mechanical Circulatory Support in Peripartum Cardiomyopathy (PPCM) Refractory to Medical Management: A Multicenter Study

Author

Jawad, Khalil, Koziarz, Alex, Dieterlen, Maja-Theresa, Garbade, Jens, Etz, Christian D., Saeed, Diyar, Langer, Elena, Stepan, Holger, Scholz, Ute, Krause, Michael, Brenner, Paolo, Schulz, Uwe, Borger, Michael A., Eifert, Sandra, Jawad, Khalil, Koziarz, Alex, Dieterlen, Maja-Theresa, Garbade, Jens, Etz, Christian D., Saeed, Diyar, Langer, Elena, Stepan, Holger, Scholz, Ute, Krause, Michael, Brenner, Paolo, Schulz, Uwe, Borger, Michael A., and Eifert, Sandra

Abstract

Background: Peripartum cardiomyopathy (PPCM) is a rare, life-threatening form of heart disease, frequently associated with gene alterations and, in some cases, presenting with advanced heart failure. Little is known about ventricular assist device (VAD) implantation in severe PPCM cases. We describe long-term follow-up of PPCM patients who were resistant to medical therapy and received mechanical circulatory support or heart transplant. Methods and results: A total of 13 patients were included with mean follow-up of eight years. Mean age of PPCM onset was 33.7 ± 7.7 years. All patients were initially treated with angiotensin-converting enzyme inhibitors and beta-blockers, and four received bromocriptine. Overall, five patients received VADs (three biventricular, two isolated left ventricular) at median 27 days (range: 3 to 150) following childbirth. Two patients developed drive line infection. Due to the short support time, none of those patients had a stroke or VAD thrombosis. In total, five patients underwent heart transplantation, of which four previously had implanted VADs. Median time to transplantation from PPCM onset was 140 days (range: 43 to 776), and time to transplantation from VAD implantation were 7, 40, 132, and 735 days, respectively. All patients survived until most recent follow up, with the exception of one patient who died following unrelated abdominal surgery two years after PPCM recovery. Conclusions: In patients with severe, life-threatening PPCM refractory to medical management, mechanical circulatory support with or without heart transplantation is a safe therapeutic option.

Published
2022

21. Long-Term Follow-Up of Mechanical Circulatory Support in Peripartum Cardiomyopathy (PPCM) Refractory to Medical Management:A Multicenter Study

Author

Jawad, Khalil, Koziarz, Alex, Dieterlen, Maja-Theresa, Garbade, Jens, Etz, Christian D., Saeed, Diyar, Langer, Elena, Stepan, Holger, Scholz, Ute, Krause, Michael, Brenner, Paolo, Schulz, Uwe, Borger, Michael A., Eifert, Sandra, Jawad, Khalil, Koziarz, Alex, Dieterlen, Maja-Theresa, Garbade, Jens, Etz, Christian D., Saeed, Diyar, Langer, Elena, Stepan, Holger, Scholz, Ute, Krause, Michael, Brenner, Paolo, Schulz, Uwe, Borger, Michael A., and Eifert, Sandra

Abstract

Background: Peripartum cardiomyopathy (PPCM) is a rare, life-threatening form of heart disease, frequently associated with gene alterations and, in some cases, presenting with advanced heart failure. Little is known about ventricular assist device (VAD) implantation in severe PPCM cases. We describe long-term follow-up of PPCM patients who were resistant to medical therapy and received mechanical circulatory support or heart transplant. Methods and results: A total of 13 patients were included with mean follow-up of eight years. Mean age of PPCM onset was 33.7 +/- 7.7 years. All patients were initially treated with angiotensin-converting enzyme inhibitors and beta-blockers, and four received bromocriptine. Overall, five patients received VADs (three biventricular, two isolated left ventricular) at median 27 days (range: 3 to 150) following childbirth. Two patients developed drive line infection. Due to the short support time, none of those patients had a stroke or VAD thrombosis. In total, five patients underwent heart transplantation, of which four previously had implanted VADs. Median time to transplantation from PPCM onset was 140 days (range: 43 to 776), and time to transplantation from VAD implantation were 7, 40, 132, and 735 days, respectively. All patients survived until most recent follow up, with the exception of one patient who died following unrelated abdominal surgery two years after PPCM recovery. Conclusions: In patients with severe, life-threatening PPCM refractory to medical management, mechanical circulatory support with or without heart transplantation is a safe therapeutic option.

Published
2022

22. Author Correction: Consistent success in life-supporting porcine cardiac xenotransplantation

Author

Längin, Matthias, Mayr, Tanja, Reichart, Bruno, Michel, Sebastian, Buchholz, Stefan, Guethoff, Sonja, Dashkevich, Alexey, Baehr, Andrea, Egerer, Stefanie, Bauer, Andreas, Mihalj, Maks, Panelli, Alessandro, Issl, Lara, Ying, Jiawei, Fresch, Ann Kathrin, Buttgereit, Ines, Mokelke, Maren, Radan, Julia, Werner, Fabian, Lutzmann, Isabelle, Steen, Stig, Sjöberg, Trygve, Paskevicius, Audrius, Qiuming, Liao, Sfriso, Riccardo, Rieben, Robert, Dahlhoff, Maik, Kessler, Barbara, Kemter, Elisabeth, Kurome, Mayuko, Zakhartchenko, Valeri, Klett, Katharina, Hinkel, Rabea, Kupatt, Christian, Falkenau, Almuth, Reu, Simone, Ellgass, Reinhard, Herzog, Rudolf, Binder, Uli, Wich, Günter, Skerra, Arne, Ayares, David, Kind, Alexander, Schönmann, Uwe, Kaup, Franz-Josef, Hagl, Christian, Wolf, Eckhard, Klymiuk, Nikolai, Brenner, Paolo, and Abicht, Jan-Michael

Published
2019
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23. Long-Term Follow-Up of Mechanical Circulatory Support in Peripartum Cardiomyopathy (PPCM) Refractory to Medical Management: A Multicenter Study

Author

Jawad, Khalil, primary, Koziarz, Alex, additional, Dieterlen, Maja-Theresa, additional, Garbade, Jens, additional, Etz, Christian D., additional, Saeed, Diyar, additional, Langer, Elena, additional, Stepan, Holger, additional, Scholz, Ute, additional, Krause, Michael, additional, Brenner, Paolo, additional, Schulz, Uwe, additional, Borger, Michael A., additional, and Eifert, Sandra, additional

Published
2022
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24. Clinical cardiac xenotransplantation first in the clinical arena

Author

Bender, Martin, primary, Längin, Matthias, additional, Reichart, Bruno, additional, Mokelke, Maren, additional, Radan, Julia, additional, Neumann, Elisabeth, additional, Michel, Sebastian, additional, Ellgass, Reinhard, additional, Cowan, Peter J., additional, Wolf, Eckhard, additional, Abicht, Jan‐Michael, additional, and Brenner, Paolo, additional

Published
2022
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27. Pre-clinical heterotopic intrathoracic heart xenotransplantation: a possibly useful clinical technique

Author

Abicht, Jan-Michael, Mayr, Tanja, Reichart, Bruno, Buchholz, Stefan, Werner, Fabian, Lutzmann, Isabelle, Schmoeckel, Michael, Bauer, Andreas, Thormann, Michael, Langenmayer, Martin, Herbach, Nadja, Pohla, Heike, Herzog, Rudolf, McGregor, Christopher G. A., Ayares, David, Wolf, Eckhard, Klymiuk, Nikolai, Baehr, Andrea, Kind, Alexander, Hagl, Christian, Ganswindt, Ute, Belka, Claus, Guethoff, Sonja, and Brenner, Paolo

Published
2015
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34. Cold non‐ischemic heart preservation with continuous perfusion prevents early graft failure in orthotopic pig‐to‐baboon xenotransplantation

Author

Längin, Matthias, primary, Reichart, Bruno, additional, Steen, Stig, additional, Sjöberg, Trygve, additional, Paskevicius, Audrius, additional, Liao, Qiuming, additional, Qin, Guangqi, additional, Mokelke, Maren, additional, Mayr, Tanja, additional, Radan, Julia, additional, Issl, Lara, additional, Buttgereit, Ines, additional, Ying, Jiawei, additional, Fresch, Ann Kathrin, additional, Panelli, Alessandro, additional, Egerer, Stefanie, additional, Bähr, Andrea, additional, Kessler, Barbara, additional, Milusev, Anastasia, additional, Sfriso, Riccardo, additional, Rieben, Robert, additional, Ayares, David, additional, Murray, Peter J., additional, Ellgass, Reinhard, additional, Walz, Christoph, additional, Klymiuk, Nikolai, additional, Wolf, Eckhard, additional, Abicht, Jan‐Michael, additional, and Brenner, Paolo, additional

Published
2020
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36. Impact of porcine cytomegalovirus on long-term orthotopic cardiac xenotransplant survival

Author

Denner, Joachim, primary, Längin, Matthias, additional, Reichart, Bruno, additional, Krüger, Luise, additional, Fiebig, Uwe, additional, Mokelke, Maren, additional, Radan, Julia, additional, Mayr, Tanja, additional, Milusev, Anastasia, additional, Luther, Fabian, additional, Sorvillo, Nicoletta, additional, Rieben, Robert, additional, Brenner, Paolo, additional, Walz, Christoph, additional, Wolf, Eckhard, additional, Roshani, Berit, additional, Stahl-Hennig, Christiane, additional, and Abicht, Jan-Michael, additional

Published
2020
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37. Hemodynamic evaluation of anesthetized baboons and piglets by transpulmonary thermodilution: Normal values and interspecies differences with respect to xenotransplantation

Author

Längin, Matthias, primary, Konrad, Mark, additional, Reichart, Bruno, additional, Mayr, Tanja, additional, Vandewiele, Stephanie, additional, Postrach, Johannes, additional, Mokelke, Maren, additional, Radan, Julia, additional, Brenner, Paolo, additional, Bauer, Andreas, additional, and Abicht, Jan‐Michael, additional

Published
2019
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44. Hemodynamic evaluation of anesthetized baboons and piglets by transpulmonary thermodilution: Normal values and interspecies differences with respect to xenotransplantation.

Author

Längin, Matthias, Konrad, Mark, Reichart, Bruno, Mayr, Tanja, Vandewiele, Stephanie, Postrach, Johannes, Mokelke, Maren, Radan, Julia, Brenner, Paolo, Bauer, Andreas, and Abicht, Jan‐Michael

Subjects
BODY surface area, BABOONS, HEMODYNAMICS, PIGLETS, XENOTRANSPLANTATION, ANIMAL welfare
Abstract

Background: Transpulmonary thermodilution is well established as a tool for in‐depth hemodynamic monitoring of critically ill patients during surgical procedures and intensive care. It permits easy assessment of graft function following cardiac transplantation and guides post‐operative volume and catecholamine therapy. Since no pulmonary catheter is needed, transpulmonary thermodilution could be useful in experimental cardiac pig‐to‐baboon xenotransplantation. However, normal values for healthy animals have not yet been reported. Here, we present data from piglets and baboons before xenotransplantation experiments and highlight differences between the two species and human reference values. Methods: Transpulmonary thermodilution from baboons (body weight 10‐34 kg) and piglets (body weight 10‐38kg) were analyzed. Measurements were taken in steady state after induction of general anesthesia before surgical procedures commenced. Cardiac index (CI), mean arterial pressure (MAP), systemic vascular resistance index (SVRI), parameters quantifying cardiac filling (global end‐diastolic volume index, GEDI), and pulmonary edema (extravascular lung water, ELWI) were assessed. Results: Preload, afterload, and contractility parameters clearly correlated with total body weight or body surface area. Baboons had lower CI values than weight‐matched piglets (4.2 ± 0.9l/min/m2 vs 5.3 ± 1.0/min/m2, P <.01). MAP and SVRI were higher in baboons than piglets (MAP: 99 ± 22 mm Hg vs 62 ± 11 mm Hg, P <.01; SVRI: 1823 ± 581 dyn*s/cm5*m2 vs 827 ± 204 dyn*s/cm5*m2, P <.01). GEDI and ELWI did differ significantly between both species, but measurements were within similar ranges (GEDI: 523 ± 103 mL/m2 vs 433 ± 78 mL/m2, P <.01; ELWI: 10 ± 3 mL/kg vs 11 ± 2 mL/kg, P <.01). Regarding adult human reference values, CI was similar to both baboons and piglets, but all other parameters were different. Conclusions: Parameters of preload, afterload, and contractility differ between baboons and piglets. In particular, baboons have a much higher afterload than piglets, which might be instrumental in causing perioperative xenograft dysfunction and post‐operative myocardial hypertrophy after orthotopic pig‐to‐baboon cardiac xenotransplantation. Most transpulmonary thermodilution‐derived parameters obtained from healthy piglets and baboons lie outside the reference ranges for humans, so human normal values should not be used to guide treatment in those animals. Our data provide reference values as a basis for developing algorithms for perioperative hemodynamic management in pig‐to‐baboon cardiac xenotransplantation. [ABSTRACT FROM AUTHOR]

Published
2020
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47. 40 Days Survival after Orthotopic Cardiac Xenotransplantation of Multi-Transgenic Pig Hearts in a Pig-to-Baboon Model with CD40mAb or CD40L Costimulation Blockade and Xenograft Preservation using “Steens” Cold Blood Cardioplegia Perfusion

Author

Brenner, Paolo, primary, Mayr, Tanja, additional, Reichart, Bruno, additional, Guethoff, Sonja, additional, Buchholz, Stefan, additional, Dashkevich, Alexander, additional, Michel, Sebastian, additional, Lutzmann, Isabelle, additional, Werner, Fabian, additional, Bauer, Andreas, additional, Klymiuk, Nikolai, additional, Wolf, Eckhard, additional, Mohiuddin, Muhammad, additional, Reimann, Keith, additional, Hermanns, Walter, additional, Ayares, David, additional, McGregor, Christopher, additional, Hagl, Christian, additional, Steen, Stig, additional, and Abicht, Jan-Michael, additional

Published
2017
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