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Additional file 3: Figure S3. Mean and standard error of the mean of anti-estrogenic activities in water samples from the river Horloff in March 2017 (a) and the river Nidda in May 2017 (b). White: reference sites, with H3R and N2R as statistical reference sites, light grey: restoration sites, dark grey: transect sites. Significant differences in anti-estrogenic activity compared to the reference site H3R (shaded) were determined using Kruskal–Wallis test with Dunn’s post hoc test. Significant differences in anti-estrogenic activity compared to the reference site N2R (shaded) were detected via one-way ANOVA and Bonferroni’s post hoc test. If no bar is illustrated, the activity was below the corresponding LOQ. *p

Additional file 3: Figure 7S6. Map of the Nidda catchment with derived ecotoxicological status classes and ecological status classes for fish and macrozoobenthos (MZB) according to HLNUG [47, 48]. Green: good status (class 2), yellow: moderate status (class 3), orange: poor status (class 4), red: bad status (class 5), white: no HLNUG data for fish or MZB available. Red spots mark dischargers from WWTPs, public baths and a paper mill, green lines restoration areas. The map was modified with Adobe® Photoshop CC (Version 20.0.0, Adobe Systems Incorporated, San José, California, US) and is based on WRRL-Viewer [24].