13 results on '"Broketa, Matteo"'
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2. High-affinity autoreactive plasma cells disseminate through multiple organs in patients with immune thrombocytopenic purpura
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Canales-Herrerias, Pablo, Crickx, Etienne, Broketa, Matteo, Sokal, Aurelien, Chenon, Guilhem, Azzaoui, Imane, Vandenberghe, Alexis, Perima, Angga, Iannascoli, Bruno, Goff, Odile Richard-Le, Castrillon, Carlos, Motte, Guillaume, Sterlin, Delphine, Robbins, Ailsa, Michel, Marc, England, Patrick, Eyer, Klaus, Millo, Gael A., Baudry, Jean, Mahevas, Matthieu, and Bruhns, Pierre
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Autoimmunity -- Health aspects ,Plasma cells -- Health aspects ,Thrombocytopenic purpura -- Complications and side effects -- Drug therapy ,Health care industry - Abstract
The major therapeutic goal for immune thrombocytopenic purpura (ITP) is to restore normal platelet counts using drugs to promote platelet production or by interfering with mechanisms responsible for platelet destruction. Eighty percent of patients with ITP possess anti-integrin [alpha]IIb[beta]3 IgG autoantibodies that cause platelet opsonization and phagocytosis. The spleen is considered the primary site of autoantibody production by autoreactive B cells and platelet destruction. The immediate failure in approximately 50% of patients to recover a normal platelet count after anti-CD20 rituximab-mediated B cell depletion and splenectomy suggests that autoreactive, rituximab-resistant, IgG-secreting B cells (IgG-SCs) reside in other anatomical compartments. We analyzed more than 3,300 single IgG-SCs from spleen, bone marrow, and/or blood of 27 patients with ITP, revealing high interindividual variability in affinity for [alpha]IIb[beta]3, with variations over 3 logs. IgG-SC dissemination and range of affinities were, however, similar for each patient. Longitudinal analysis of autoreactive IgG-SCs upon treatment with the anti-CD38 mAb daratumumab demonstrated variable outcomes, from complete remission to failure with persistence of high-affinity anti-[alpha]IIb[beta]3 IgG-SCs in the bone marrow. This study demonstrates the existence and dissemination of high-affinity autoreactive plasma cells in multiple anatomical compartments of patients with ITP that may cause the failure of current therapies., Introduction Immune thrombocytopenic purpura (ITP) is an immune disorder characterized by a strong autoimmune response against platelet autoantigens that causes platelet destruction (1). Clinical manifestations often present as mild bleeding [...]
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- 2022
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3. Qualitative monitoring of SARS-CoV-2 mRNA vaccination in humans using droplet microfluidics
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Broketa, Matteo, primary, Sokal, Aurélien, additional, Mor, Michael, additional, Canales-Herrerias, Pablo, additional, Perima, Angga, additional, Meola, Annalisa, additional, Fernández, Ignacio, additional, Iannascoli, Bruno, additional, Chenon, Guilhem, additional, Vandenberghe, Alexis, additional, Languille, Laetitia, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Backovic, Marija, additional, Rey, Felix A., additional, Baudry, Jean, additional, Freund, Natalia T., additional, Mahévas, Matthieu, additional, and Bruhns, Pierre, additional
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- 2023
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4. Omicron BA.1 breakthrough infection drives long-term remodeling of the memory B cell repertoire in vaccinated individuals
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Sokal, Aurélien, primary, Barba-Spaeth, Giovanna, additional, Hunault, Lise, additional, Fernández, Ignacio, additional, Broketa, Matteo, additional, Meola, Annalisa, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, Vandenberghe, Alexis, additional, Lagouge-Roussey, Pauline, additional, Broutin, Manon, additional, Roeser, Anais, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Fournier, Morgane, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Reynaud, Claude-Agnès, additional, Weill, Jean-Claude, additional, Rey, Félix A., additional, Bruhns, Pierre, additional, Mahévas, Matthieu, additional, and Chappert, Pascal, additional
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- 2023
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5. Omicron BA.1 breakthrough infection drives long-term remodeling of the memory B cell repertoire in vaccinated individuals
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Sokal, Aurélien, Barba-Spaeth, Giovanna, Hunault, Lise, Fernández, Ignacio, Broketa, Matteo, Meola, Annalisa, Fourati, Slim, Azzaoui, Imane, Vandenberghe, Alexis, Lagouge-Roussey, Pauline, Broutin, Manon, Roeser, Anais, Bouvier-Alias, Magali, Crickx, Etienne, Languille, Laetitia, Fournier, Morgane, Michel, Marc, Godeau, Bertrand, Gallien, Sébastien, Melica, Giovanna, Nguyen, Yann, Canoui-Poitrine, Florence, Noizat-Pirenne, France, Megret, Jérôme, Pawlotsky, Jean-Michel, Fillatreau, Simon, Reynaud, Claude-Agnès, Weill, Jean-Claude, Rey, Félix, Bruhns, Pierre, Mahévas, Matthieu, Chappert, Pascal, Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CIC - CHU Henri Mondor, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Physiologie, physiopathologie et thérapeutique (ED 394), Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Henri Mondor [Créteil], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Cochin [AP-HP], IMRB - CEPIA/'Clinical Epidemiology And Ageing : Geriatrics, Primary Care and Public Health' [Créteil] (U955 Inserm - UPEC), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Etablissement Français du Sang [Créteil], Structure Fédérative de Recherche Necker (SFR Necker - UMS 3633 / US24), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and This work was funded by the CAPNET (Comité ad-hoc de pilotage national des essaisthérapeutiques et autres recherches, French government) and by the Fondation PrincesseGrace. Assistance Publique – Hôpitaux de Paris (AP-HP, Département de la Recherche Cli-nique et du Développement) was the promotor and sponsor of MEMO-COV-2. Work in theUnit of Structural Virology was funded by Institut Pasteur, Urgence COVID-19 FundraisingCampaign of Institut Pasteur. PB acknowledges funding from the Institut Pasteur and InsitutNational de la Santé et de la Recherche Médicale (INSERM). AS was supported by a Posted’accueil from INSERM.
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[SDV]Life Sciences [q-bio] ,[SDV.IMM]Life Sciences [q-bio]/Immunology - Abstract
SummaryHow infection by a viral variant showing antigenic drift impacts a preformed mature human memory B cell (MBC) repertoire remains an open question. Here, we studied the MBC response up to 6 months after Omicron BA.1 breakthrough infection in individuals previously vaccinated with three doses of mRNA vaccine. Longitudinal analysis, using single-cell multi-omics and functional analysis of monoclonal antibodies from RBD-specific MBCs, revealed that a BA.1 breakthrough infection mostly recruited pre-existing cross-reactive MBCs with limitedde novoresponse against BA.1-restricted epitopes. Reorganization of clonal hierarchy and new rounds of germinal center reaction, however, combined to maintain diversity and induce progressive maturation of the MBC repertoire against common Hu-1 and BA.1, but not BA.5-restricted, SARS-CoV-2 Spike RBD epitopes. Such remodeling was further associated with marked improvement in overall neutralizing breadth and potency. These findings have fundamental implications for the design of future vaccination booster strategies.
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- 2023
6. Antibodies as drugs—a Keystone Symposia report
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Cable, Jennifer, primary, Saphire, Erica Ollmann, additional, Hayday, Adrian C., additional, Wiltshire, Timothy D., additional, Mousa, Jarrod J., additional, Humphreys, David P., additional, Breij, Esther C. W., additional, Bruhns, Pierre, additional, Broketa, Matteo, additional, Furuya, Genta, additional, Hauser, Blake M., additional, Mahévas, Matthieu, additional, Carfi, Andrea, additional, Cantaert, Tineke, additional, Kwong, Peter D., additional, Tripathi, Prabhanshu, additional, Davis, Jonathan H., additional, Brewis, Neil, additional, Keyt, Bruce A., additional, Fennemann, Felix L., additional, Dussupt, Vincent, additional, Sivasubramanian, Arvind, additional, Kim, Philip M., additional, Rawi, Reda, additional, Richardson, Eve, additional, Leventhal, Daniel, additional, Wolters, Rachael M., additional, Geuijen, Cecile A. W., additional, Sleeman, Matthew A., additional, Pengo, Niccolo, additional, and Donnellan, Francesca Rose, additional
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- 2022
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7. Analysis of mRNA vaccination-elicited RBD-specific memory B cells reveals strong but incomplete immune escape of the SARS-CoV-2 Omicron variant
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Sokal, Aurélien, primary, Broketa, Matteo, additional, Barba-Spaeth, Giovanna, additional, Meola, Annalisa, additional, Fernández, Ignacio, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, de La Selle, Andrea, additional, Vandenberghe, Alexis, additional, Roeser, Anais, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Zarrouk, Virginie, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Simon-Lorière, Etienne, additional, Weill, Jean-Claude, additional, Reynaud, Claude-Agnès, additional, Rey, Félix A., additional, Bruhns, Pierre, additional, Chappert, Pascal, additional, and Mahévas, Matthieu, additional
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- 2022
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8. Antibodies as drugs—a Keystone Symposia report.
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Cable, Jennifer, Saphire, Erica Ollmann, Hayday, Adrian C., Wiltshire, Timothy D., Mousa, Jarrod J., Humphreys, David P., Breij, Esther C. W., Bruhns, Pierre, Broketa, Matteo, Furuya, Genta, Hauser, Blake M., Mahévas, Matthieu, Carfi, Andrea, Cantaert, Tineke, Kwong, Peter D., Tripathi, Prabhanshu, Davis, Jonathan H., Brewis, Neil, Keyt, Bruce A., and Fennemann, Felix L.
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BISPECIFIC antibodies ,MONOCLONAL antibodies ,DEEP learning ,CONFERENCES & conventions ,AUTOIMMUNE diseases ,DISEASE progression ,IMMUNOGLOBULINS - Abstract
Therapeutic antibodies have broad indications across diverse disease states, such as oncology, autoimmune diseases, and infectious diseases. New research continues to identify antibodies with therapeutic potential as well as methods to improve upon endogenous antibodies and to design antibodies de novo. On April 27–30, 2022, experts in antibody research across academia and industry met for the Keystone symposium "Antibodies as Drugs" to present the state‐of‐the‐art in antibody therapeutics, repertoires and deep learning, bispecific antibodies, and engineering. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Single-Cell Technologies for the Study of Antibody-Secreting Cells
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Broketa, Matteo, primary and Bruhns, Pierre, additional
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- 2022
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10. Immune escape of SARS-CoV-2 Omicron variant from mRNA vaccination-elicited RBD-specific memory B cells.
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Sokal, Aurelien, primary, Broketa, Matteo, additional, Meola, Annalisa, additional, Barba-Spaeth, Giovanna, additional, Fernandez, Ignacio, additional, Fourati, Slim, additional, Azzaoui, Imane, additional, de La Selle, Andrea, additional, Vandenberghe, Alexis, additional, Roeser, Anais, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sebastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Zarrouk, Virginie, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jerome, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Simon-Loriere, Etienne, additional, Weill, Jean-Claude, additional, Reynaud, Claude-Agnes, additional, Rey, Felix A., additional, Bruhns, Pierre, additional, Chappert, Pascal, additional, and Mahevas, Matthieu, additional
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- 2021
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11. mRNA vaccination of naive and COVID-19-recovered individuals elicits potent memory B cells that recognize SARS-CoV-2 variants
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Sokal, Aurélien, primary, Barba-Spaeth, Giovanna, additional, Fernández, Ignacio, additional, Broketa, Matteo, additional, Azzaoui, Imane, additional, de La Selle, Andréa, additional, Vandenberghe, Alexis, additional, Fourati, Slim, additional, Roeser, Anais, additional, Meola, Annalisa, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Zarrouk, Virginie, additional, Canoui-Poitrine, Florence, additional, Pirenne, France, additional, Mégret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Bruhns, Pierre, additional, Rey, Felix A., additional, Weill, Jean-Claude, additional, Reynaud, Claude-Agnès, additional, Chappert, Pascal, additional, and Mahévas, Matthieu, additional
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- 2021
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12. Single-cell analyses of immune thrombocytopenic patients reveal multiorgan dissemination of high-affinity autoreactive plasma cells
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Canales-Herrerias, Pablo, primary, Crickx, Etienne, additional, Broketa, Matteo, additional, Sokal, Aurélien, additional, Chenon, Guilhem, additional, Azzaoui, Imane, additional, Vandenberghe, Alexis, additional, Perima, Angga, additional, Iannascoli, Bruno, additional, Goff, Odile Richard-Le, additional, Castrillon, Carlos, additional, Mottet, Guillaume, additional, Sterlin, Delphine, additional, Robbins, Ailsa, additional, Michel, Marc, additional, England, Patrick, additional, Millot, Gael A., additional, Eyer, Klaus, additional, Baudry, Jean, additional, Mahevas, Matthieu, additional, and Bruhns, Pierre, additional
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- 2021
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13. Memory B cells control SARS-CoV-2 variants upon mRNA vaccination of naive and COVID-19 recovered individuals
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Sokal, Aurélien, primary, Barba-Spaeth, Giovanna, additional, Fernández, Ignacio, additional, Broketa, Matteo, additional, Azzaoui, Imane, additional, La Selle, Andrea de, additional, Vandenberghe, Alexis, additional, Fourati, Slim, additional, Roeser, Anais, additional, Meola, Annalisa, additional, Bouvier-Alias, Magali, additional, Crickx, Etienne, additional, Languille, Laetitia, additional, Michel, Marc, additional, Godeau, Bertrand, additional, Gallien, Sébastien, additional, Melica, Giovanna, additional, Nguyen, Yann, additional, Zarrouk, Virginie, additional, Canoui-Poitrine, Florence, additional, Noizat-Pirenne, France, additional, Megret, Jérôme, additional, Pawlotsky, Jean-Michel, additional, Fillatreau, Simon, additional, Bruhns, Pierre, additional, Rey, Felix A., additional, Weill, Jean-Claude, additional, Reynaud, Claude-Agnès, additional, Chappert, Pascal, additional, and Mahévas, Matthieu, additional
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- 2021
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