1. Development and validation of a TaqMan® PCR assay for the Australian abalone herpes-like virus
- Author
-
Pen H. Chang, Bronwyn Murdoch, Noel O. I. Cogan, Stephen Pyecroft, Simone Warner, Serge Corbeil, Ilhan Mohammad, Mark Fegan, Mark St. J. Crane, Judith Handlinger, A. Colling, Lynette M. Williams, Frank Y. K. Wong, Agus Sunarto, Marianne Douglas, Tim Sawbridge, and Keith W. Savin
- Subjects
medicine.medical_specialty ,Abalone ,Aquatic Science ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Genome ,Virus ,law.invention ,law ,Molecular genetics ,Herpesvirales ,medicine ,TaqMan ,Animals ,Herpesviridae ,Ecology, Evolution, Behavior and Systematics ,Whole genome sequencing ,biology ,Australia ,Reproducibility of Results ,biology.organism_classification ,Virology ,Molecular biology ,Mollusca ,DNA, Viral ,Recombinant DNA - Abstract
The recent emergence of a herpes-like virus in both farmed and wild populations of abalone in Victoria, Australia, has been associated with high mortality rates in animals of all ages. Based on viral genome sequence information, a virus-specific real-time TaqMan assay was devel- oped for detection and identification of the abalone herpes-like virus (AbHV). The assay was shown to be specific as it did not detect other viruses from either the Herpesvirales or the Iridovirales orders which have genome sequence similarities. However, the TaqMan assay was able to detect DNA from the Taiwanese abalone herpes-like virus, suggesting a relationship between the Taiwanese and Aus- tralian viruses. In addition, the assay detected
- Published
- 2010
- Full Text
- View/download PDF