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14 results on '"Brown, HannahSofia"'

5. 1194. Efficacy of shortened high-level disinfection (HLD) protocols for GI duodenoscopes with disposable tips used for endoscopic retrograde cholangiopancreatography (ERCP)

Author

Warren, Bobby G, primary, Muralidharan, Sriya, additional, Brown, HannahSofia, additional, Barrett, Aaron, additional, Graves, Amanda M, additional, King, Carly, additional, Mohammed, Fahad S, additional, Triplin, Carlos, additional, Anderson, Deverick J, additional, Seidelman, Jessica, additional, Smith, Becky A, additional, and Dufault, Darin, additional

Published
2022
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6. S1081 Evaluation of Abbreviated HLD Protocols for Duodenoscopes With Disposable Tips

Author

Muralidharan, Sriya, primary, Parish, Alice, additional, Niedzwiecki, Donna, additional, Warren, Bobby G., additional, Barrett, Aaron, additional, Brown, HannahSofia, additional, Mohammed, Fahad S., additional, Graves, Amanda, additional, King, Carly, additional, Triplin, Carlos, additional, Seidelman, Jessica, additional, Smith, Becky, additional, and Dufault, Darin, additional

Published
2022
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7. S1071 Assessment of Pathogenic Enteric Flora Contaminating Novel Duodenoscopes With Disposable Tips

Author

Muralidharan, Sriya, primary, Parish, Alice, additional, Niedzwiecki, Donna, additional, Warren, Bobby G., additional, Brown, HannahSofia, additional, Mohammed, Fahad S., additional, Barrett, Aaron, additional, Graves, Amanda, additional, King, Carly, additional, Triplin, Carlos, additional, Seidelman, Jessica, additional, Smith, Becky, additional, and Dufault, Darin, additional

Published
2022
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9. Fission yeast Opy1 is an endogenous PI(4,5)P(2) sensor that binds to the phosphatidylinositol 4-phosphate 5-kinase Its3

Author

Snider, Chloe E., Willet, Alaina H., Brown, HannahSofia T., Chen, Jun-Song, Evers, Joshua M., and Gould, Kathleen L.

Subjects
Phosphatidylinositol 4,5-Diphosphate, Phosphatidylinositol Phosphates, Cell Membrane, Schizosaccharomyces, Short Report, Phosphatidylinositols
Abstract

Phosphoinositides (PIPs) are a dynamic family of lipids that execute diverse roles in cell biology. PIP levels are regulated by numerous enzymes, but our understanding of how these enzymes are controlled in space and time is incomplete. One role of the PIP phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] is to anchor the cytokinetic ring (CR) to the plasma membrane (PM) in Schizosaccharomyces pombe. While examining potential PI(4,5)P(2)-binding proteins for roles in CR anchoring, we identified the dual pleckstrin homology (PH) domain-containing protein Opy1. Although related proteins are implicated in PIP regulation, we found no role for S. pombe Opy1 in CR anchoring, which would be expected if it modulated PM PI(4,5)P(2) levels. Our data indicate that although Opy1 senses PM PI(4,5)P(2) levels and binds to the phosphatidylinositol 4-phosphate 5-kinase (PI5-kinase) Its3, Opy1 does not regulate Its3 kinase activity or PM PI(4,5)P(2) levels, a striking difference from its Saccharomyces cerevisiae homolog. However, overexpression of Opy1 resulted in cytokinesis defects, as might be expected if it sequestered PI(4,5)P(2). Our results highlight the evolutionary divergence of dual PH domain-containing proteins and the need for caution when interpreting results based on their overexpression. This article has an associated First Person interview with the first author of the paper.

Published
2020

14. Analysis of the contribution of phosphoinositides to medial septation in fission yeast highlights the importance of PI(4,5)P 2 for medial contractile ring anchoring.

Author

Snider CE, Willet AH, Brown HT, and Gould KL

Subjects
Actins, Anaphase physiology, Cell Cycle Proteins, Cell Division physiology, Cell Nucleus, Cytoplasm, Mitosis physiology, Phosphatidylinositols metabolism, Phosphatidylinositols physiology, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins metabolism, Cytokinesis physiology, Phosphatidylinositol 4,5-Diphosphate metabolism, Phosphatidylinositol 4,5-Diphosphate physiology
Abstract

In Schizosaccharomyces pombe, loss of the plasma membrane PI4-kinase scaffold Efr3 leads to sliding of the cytokinetic ring (CR) away from the cell center during anaphase, implicating phosphoinositides (PIPs) in CR anchoring. However, whether other PIP regulators contribute to CR anchoring has not been investigated. Here we report that mutants of other PIP kinases and their regulators divide with off-center septa, similar to efr3∆. Using new biosensors for S. pombe PIPs, we confirm that these mutants have disrupted PIP composition. We extend a previous finding that a mutant known to decrease PI(3,5)P 2 levels indirectly affects CR positioning by increasing vacuole size which disrupts nuclear position at the onset of mitosis. Indeed, we found that other mutants with increased vacuole size also disrupt medial division via this mechanism. Although elevated plasma membrane PI(4,5)P 2 levels do not affect medial cytokinesis, mutants with decreased levels display CR sliding events indicating a specific role for PI(4,5)P 2 in CR anchoring.

Published
2018
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