957 results on '"Brown adipocytes"'
Search Results
2. Neuronostatin regulates proliferation and differentiation of rat brown primary preadipocytes.
- Author
-
Krążek, Małgorzata, Wojciechowicz, Tatiana, Fiedorowicz, Joanna, Strowski, Mathias Z., Nowak, Krzysztof W., and Skrzypski, Marek
- Subjects
- *
BROWN adipose tissue , *RATTUS norvegicus , *SOMATOSTATIN , *FAT cells , *CELLULAR signal transduction - Abstract
Neuronostatin suppresses the differentiation of white preadipocytes. However, the role of neuronostatin in brown adipose tissue remains elusive. Therefore, we investigated the impact of neuronostatin on the proliferation and differentiation of isolated rat brown preadipocytes. We report that neuronostatin and its receptor (GPR107) are synthesized in brown preadipocytes and brown adipose tissue. Furthermore, neuronostatin promotes the replication of brown preadipocytes via the AKT pathway. Notably, neuronostatin suppresses the expression of markers associated with brown adipogenesis (PGC‐1α, PPARγ, PRDM16, and UCP1) and reduces cellular mitochondria content. Moreover, neuronostatin impedes the differentiation of preadipocytes by activating the JNK signaling pathway. These effects were not mimicked by somatostatin. Our results suggest that neuronostatin is involved in regulating brown adipogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Exploring the anti-obesity effects of kimchi through enhanced thermogenesis in differentiated T37i brown adipocytes
- Author
-
Ye-Rang Yun, Ji-Eun Lee, Seongsoo Lee, and Sung Wook Hong
- Subjects
kimchi ,obesity ,thermogenesis ,brown adipocytes ,gene expression ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background: Previous research has demonstrated the anti-obesity effects of kimchi in 3T3-L1 adipocytes and mice with diet-induced obesity by assessing the expression of obesity-associated genes. Additionally, recent studies have identified mechanisms involving thermogenesis that support these effects. Objective: This study aims to further investigate the anti-obesity properties of kimchi, focusing on its impact on thermogenic activity in differentiated T37i brown adipocytes. Design: The study first evaluated the antioxidant potential of kimchi using total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) assays. Optimal differentiation conditions for T37i adipocytes were established before proceeding with evaluations of cell viability, intracellular triglyceride (TG) content, lipid accumulation, and the expression of genes and proteins related to obesity and thermogenesis. Results: Kimchi maintained over 90% cell viability in T37i adipocytes at concentrations up to 1,000 μg/mL. Efficient differentiation of T37i preadipocytes was achieved using a medium containing 10% calf serum, 2 nM 3,3’,5-triiodo-L-thyronin (T3), and 100 nM insulin. Kimchi significantly reduced intracellular TG levels and lipid accumulation, compared to the control group, and enhanced the expression of genes and proteins related to thermogenesis while reducing the expression of obesity-related genes. Discussion: The findings suggest that kimchi exerts its anti-obesity effects by modulating thermogenic and obesity-related pathways in brown adipocytes, which may be partially attributed to its antioxidant properties. Conclusions: Kimchi shows promise as a preventive measure against obesity by influencing metabolic pathways associated with both obesity and thermogenesis in T37i brown adipocytes.
- Published
- 2024
- Full Text
- View/download PDF
4. Functional roles of pantothenic acid, riboflavin, thiamine, and choline in adipocyte browning in chemically induced human brown adipocytes
- Author
-
Yukimasa Takeda and Ping Dai
- Subjects
Brown adipocytes ,Vitamin B ,Choline ,Pantothenic acid ,Riboflavin ,Thiamine ,Medicine ,Science - Abstract
Abstract Brown fat is a therapeutic target for the treatment of obesity-associated metabolic diseases. However, nutritional intervention strategies for increasing the mass and activity of human brown adipocytes have not yet been established. To identify vitamins required for brown adipogenesis and adipocyte browning, chemical compound-induced brown adipocytes (ciBAs) were converted from human dermal fibroblasts under serum-free and vitamin-free conditions. Choline was found to be essential for adipogenesis. Additional treatment with pantothenic acid (PA) provided choline-induced immature adipocytes with browning properties and metabolic maturation, including uncoupling protein 1 (UCP1) expression, lipolysis, and mitochondrial respiration. However, treatment with high PA concentrations attenuated these effects along with decreased glycolysis. Transcriptome analysis showed that a low PA concentration activated metabolic genes, including the futile creatine cycle-related thermogenic genes, which was reversed by a high PA concentration. Riboflavin treatment suppressed thermogenic gene expression and increased lipolysis, implying a metabolic pathway different from that of PA. Thiamine treatment slightly activated thermogenic genes along with decreased glycolysis. In summary, our results suggest that specific B vitamins and choline are uniquely involved in the regulation of adipocyte browning via cellular energy metabolism in a concentration-dependent manner.
- Published
- 2024
- Full Text
- View/download PDF
5. Possible Applications of Cold Stimulus in Obesity and Diabetes
- Author
-
Cinti, Saverio and Capodaglio, Paolo, editor
- Published
- 2024
- Full Text
- View/download PDF
6. Angiotensin II participates in mitochondrial thermogenic functions via the activation of glycolysis in chemically induced human brown adipocytes
- Author
-
Yukimasa Takeda, Toshikazu Yoshikawa, and Ping Dai
- Subjects
Brown adipocytes ,Angiotensin II ,UCP1 ,Mitochondria ,Adaptive thermogenesis ,Glycolysis ,Medicine ,Science - Abstract
Abstract Brown adipocytes are potential therapeutic targets for the prevention of obesity-associated metabolic diseases because they consume circulating glucose and fatty acids for heat production. Angiotensin II (Ang II) peptide is involved in the pathogenesis of obesity- and cold-induced hypertension; however, the mechanism underlying the direct effects of Ang II on human brown adipocytes remains unclear. Our transcriptome analysis of chemical compound-induced brown adipocytes (ciBAs) showed that the Ang II type 1 receptor (AGTR1), but not AGTR2 and MAS1 receptors, was expressed. The Ang II/AGTR1 axis downregulated the expression of mitochondrial uncoupling protein 1 (UCP1). The simultaneous treatment with β-adrenergic receptor agonists and Ang II attenuated UCP1 expression, triglyceride lipolysis, and cAMP levels, although cAMP response element-binding protein (CREB) phosphorylation was enhanced by Ang II mainly through the protein kinase C pathway. Despite reduced lipolysis, both coupled and uncoupled mitochondrial respiration was enhanced in Ang II-treated ciBAs. Instead, glycolysis and glucose uptake were robustly activated upon treatment with Ang II without a comprehensive transcriptional change in glucose metabolic genes. Elevated mitochondrial energy status induced by Ang II was likely associated with UCP1 repression. Our findings suggest that the Ang II/AGTR1 axis participates in mitochondrial thermogenic functions via glycolysis.
- Published
- 2024
- Full Text
- View/download PDF
7. Ginsenoside Rg3, enriched in red ginseng extract, improves lipopolysaccharides-induced suppression of brown and beige adipose thermogenesis with mitochondrial activation
- Author
-
Fang Feng, Hyun-A Ko, Thi My Tien Truong, Woo-Jin Song, Eun-Ju Ko, and Inhae Kang
- Subjects
Ginsenoside Rg3 ,Beige adipocytes ,Brown adipocytes ,Adaptive thermogenesis ,Inflammation ,Mitochondrial activation ,Medicine ,Science - Abstract
Abstract Brown adipose tissue (BAT) which is a critical regulator of energy homeostasis, and its activity is inhibited by obesity and low-grade chronic inflammation. Ginsenoside Rg3, the primary constituent of Korean red ginseng (steamed Panax ginseng CA Meyer), has shown therapeutic potential in combating inflammatory and metabolic diseases. However, it remains unclear whether Rg3 can protect against the suppression of browning or activation of BAT induced by inflammation. In this study, we conducted a screening of ginsenoside composition in red ginseng extract (RGE) and explored the anti-adipogenic effects of both RGE and Rg3. We observed that RGE (exist 0.25 mg/mL of Rg3) exhibited significant lipid-lowering effects in adipocytes during adipogenesis. Moreover, treatment with Rg3 (60 μM) led to the inhibition of triglyceride accumulation, subsequently promoting enhanced fatty acid oxidation, as evidenced by the conversion of radiolabeled 3H-fatty acids into 3H-H2O with mitochondrial activation. Rg3 alleviated the attenuation of browning in lipopolysaccharide (LPS)-treated beige adipocytes and primary brown adipocytes by recovered by uncoupling protein 1 (UCP1) and the oxygen consumption rate compared to the LPS-treated group. These protective effects of Rg3 on inflammation-induced inhibition of beige and BAT-derived thermogenesis were confirmed in vivo by treating with CL316,243 (a beta-adrenergic receptor agonist) and LPS to induce browning and inflammation, respectively. Consistent with the in vitro data, treatment with Rg3 (2.5 mg/kg, 8 weeks) effectively reversed the LPS-induced inhibition of brown adipocyte features in C57BL/6 mice. Our findings confirm that Rg3-rich foods are potential browning agents that counteract chronic inflammation and metabolic complications.
- Published
- 2024
- Full Text
- View/download PDF
8. A modified system using macrophage-conditioned medium revealed that the indirect effects of anti-inflammatory food-derived compounds improve inflammation-induced suppression of UCP-1 mRNA expression in 10T1/2 adipocytes.
- Author
-
Takahashi, Hisako, Morikawa, Miori, Ozaki, Emi, Numasaki, Minami, Morimoto, Hiromu, Tanaka, Miori, Inoue, Hirofumi, Goto, Tsuyoshi, Kawada, Teruo, Eguchi, Fumio, Uehara, Mariko, and Takahashi, Nobuyuki
- Subjects
- *
GENE expression , *FAT cells , *EDIBLE mushrooms , *GINGER , *ADIPOGENESIS - Abstract
Recently, it has been suggested that brown and beige adipocytes may ameliorate obesity because these adipocytes express uncoupling protein-1 (UCP-1), which generates heat by consuming lipid. However, obesity-induced inflammation suppresses the expression of UCP-1. To improve such conditions, food components with anti-inflammatory properties are attracting attention. In this study, we developed a modified system to evaluate only the indirect effects of anti-inflammatory food-derived compounds by optimizing the conventional experimental system using conditioned medium. We validated this new system using 6-shogaol and 6-gingerol, which have been reported to show the anti-inflammatory effects and to increase the basal expression of UCP-1 mRNA. In addition, we found that the acetone extract of Sarcodon aspratus , an edible mushroom, showed anti-inflammatory effects and rescued the inflammation-induced suppression of UCP-1 mRNA expression. These findings indicate that the system with conditioned medium is valuable for evaluation of food-derived compounds with anti-inflammatory effects on the inflammation-induced thermogenic adipocyte dysfunction. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
9. Angiotensin II participates in mitochondrial thermogenic functions via the activation of glycolysis in chemically induced human brown adipocytes.
- Author
-
Takeda, Yukimasa, Yoshikawa, Toshikazu, and Dai, Ping
- Subjects
- *
LIPOLYSIS , *ANALYTICAL chemistry , *FAT cells , *PROTEIN kinase C , *ANGIOTENSIN II , *GLYCOLYSIS , *MITOCHONDRIAL proteins - Abstract
Brown adipocytes are potential therapeutic targets for the prevention of obesity-associated metabolic diseases because they consume circulating glucose and fatty acids for heat production. Angiotensin II (Ang II) peptide is involved in the pathogenesis of obesity- and cold-induced hypertension; however, the mechanism underlying the direct effects of Ang II on human brown adipocytes remains unclear. Our transcriptome analysis of chemical compound-induced brown adipocytes (ciBAs) showed that the Ang II type 1 receptor (AGTR1), but not AGTR2 and MAS1 receptors, was expressed. The Ang II/AGTR1 axis downregulated the expression of mitochondrial uncoupling protein 1 (UCP1). The simultaneous treatment with β-adrenergic receptor agonists and Ang II attenuated UCP1 expression, triglyceride lipolysis, and cAMP levels, although cAMP response element-binding protein (CREB) phosphorylation was enhanced by Ang II mainly through the protein kinase C pathway. Despite reduced lipolysis, both coupled and uncoupled mitochondrial respiration was enhanced in Ang II-treated ciBAs. Instead, glycolysis and glucose uptake were robustly activated upon treatment with Ang II without a comprehensive transcriptional change in glucose metabolic genes. Elevated mitochondrial energy status induced by Ang II was likely associated with UCP1 repression. Our findings suggest that the Ang II/AGTR1 axis participates in mitochondrial thermogenic functions via glycolysis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. mitoNEET 过表达抑制铁死亡引起的棕色脂肪 细胞线粒体代谢功能障碍.
- Author
-
郑红玉, 田 震, 王燕霞, 周 坤, 任 重, 周支香, 熊文昊, 郑 核, and 姜志胜
- Abstract
AIM: To investigate the potential impact of mitoNEET [mitochondrial protein containing Asn-GluGlu-Thr (NEET) sequence] on mitochondrial metabolism in brown adipocytes, and to elucidate its underlying mechanism. METHODS: An in vitro model of primary mouse brown adipocytes was established. Western blot were utilized to detect relevant proteins, and iron ion and ATP content was measured using kits. Mitochondrial membrane potential and reactive oxygen species (ROS) were assessed by fluorescence microscopy and flow cytometry. RESULTS: The expression of the ferroptosis-related protein ACSL4 increased by 1. 13 times in ferroptosis inducer erastin treatment group, whereas the expression of SLC7A11 and GPX4 decreased by 27. 33% and 25. 33%, respectively, compared with control group (P< 0. 05). The expression of Nrf1, PGC-1α, MFN2 and UCP1 proteins, related to mitochondrial energy metabolism, decreased by 20. 98%, 15. 17%, 15. 03% and 34. 22%, respectively (P<0. 05). Additionally, the mitoNEET protein content was significantly reduced by 42. 14% (P<0. 05). The iron ion content in erastin group was substantially increased by 1. 80 times compared with control group. However, a notable decrease in ATP content of 14. 95% was seen (P<0. 05). The results obtained from fluorescence microscopy and flow cytometry demonstrated a significant decrease in the mitochondrial membrane potential of brown adipocytes in erastin group, with reductions of 52. 18% and 61. 31% (P<0. 05), respectively. A substantial increase in mitochondrial ROS content of 80. 97% was seen (P<0. 05). Western blot analysis of overexpressed stable strains revealed a significant elevation in mitoNEET levels in brown adipocytes following lentivirus transfection, exhibiting an increase of 11. 19 times (P<0. 05), thus confirming successful transfection. The LV-mitoNEET group exhibited a significant decrease of 37. 95% in the expression of ferroptosis-related protein ACSL4 in brown adipose cells compared with control group. Additionally, there was a notable increase of 77. 82% and 66. 3% in the expression of SLC7A11 and GPX4, respectively (P<0. 05). Up-regulation was observed in the expression of MFN2 (79. 06%), PGC1α (72. 89%), Nrf1 (40. 14%), and UCP1 (31. 68%) (P<0. 05). The test results demonstrated that the LV-mitoNEET group experienced a reduction of 43. 5% in iron ion content compared with control group while exhibiting an increase of 33. 5% in ATP content (P<0. 05). The results obtained from fluorescence microscopy and flow cytometry demonstrated that mitoNEET overexpression led to a significant increase in the mitochondrial membrane potential of erastin-induced brown adipocytes, with increments of 17. 61% and 96. 05%, respectively. Additionally, mitoNEET overexpression effectively reduced the production of mitochondrial ROS by 24. 48% (P<0. 05). CONCLUSION: Our findings suggest that mitoNEET overexpression can effectively inhibit the disruption of mitochondrial energy metabolism caused by ferroptosisinduced death of brown adipocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
11. Ginsenoside Rg3, enriched in red ginseng extract, improves lipopolysaccharides-induced suppression of brown and beige adipose thermogenesis with mitochondrial activation.
- Author
-
Feng, Fang, Ko, Hyun-A, Truong, Thi My Tien, Song, Woo-Jin, Ko, Eun-Ju, and Kang, Inhae
- Subjects
- *
GINSENG , *GINSENOSIDES , *BROWN adipose tissue , *BODY temperature regulation , *BETA adrenoceptors , *FATTY acid oxidation - Abstract
Brown adipose tissue (BAT) which is a critical regulator of energy homeostasis, and its activity is inhibited by obesity and low-grade chronic inflammation. Ginsenoside Rg3, the primary constituent of Korean red ginseng (steamed Panax ginseng CA Meyer), has shown therapeutic potential in combating inflammatory and metabolic diseases. However, it remains unclear whether Rg3 can protect against the suppression of browning or activation of BAT induced by inflammation. In this study, we conducted a screening of ginsenoside composition in red ginseng extract (RGE) and explored the anti-adipogenic effects of both RGE and Rg3. We observed that RGE (exist 0.25 mg/mL of Rg3) exhibited significant lipid-lowering effects in adipocytes during adipogenesis. Moreover, treatment with Rg3 (60 μM) led to the inhibition of triglyceride accumulation, subsequently promoting enhanced fatty acid oxidation, as evidenced by the conversion of radiolabeled 3H-fatty acids into 3H-H2O with mitochondrial activation. Rg3 alleviated the attenuation of browning in lipopolysaccharide (LPS)-treated beige adipocytes and primary brown adipocytes by recovered by uncoupling protein 1 (UCP1) and the oxygen consumption rate compared to the LPS-treated group. These protective effects of Rg3 on inflammation-induced inhibition of beige and BAT-derived thermogenesis were confirmed in vivo by treating with CL316,243 (a beta-adrenergic receptor agonist) and LPS to induce browning and inflammation, respectively. Consistent with the in vitro data, treatment with Rg3 (2.5 mg/kg, 8 weeks) effectively reversed the LPS-induced inhibition of brown adipocyte features in C57BL/6 mice. Our findings confirm that Rg3-rich foods are potential browning agents that counteract chronic inflammation and metabolic complications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
12. Molecular challenges of adipocytes biomarkers related obesity updates.
- Author
-
Ellethy, Abousree T. and Hagag, Mohamed E.
- Subjects
- *
FAT cells , *ADIPOSE tissues , *BIOMARKERS , *ADIPOKINES , *OBESITY , *METABOLIC disorders , *ADIPOSE tissue diseases - Abstract
Overweight is an urgent concern of majority of health organizations where it is rising incidence of metabolic syndromes and diseases. Molecular genetics studies on obesity investigated several adipocyte biomarkers with clinical significance. Increased non-blocked synthetic pathways in adipose tissues caused by excess calories consequence overweight and obese populations. Visceral fat adipocytes are strictly associated with metabolic dysfunctions, insulin resistance, heart conditions and others than subcutaneous fats. Adipocytes are the key endocrine like cells that release verities of biological protein adipokines derivatives causing different anti-inflammatory events. Disbalance in the adipokines synthesis and excretions will affect different fat tissues causing complications and pathogenesis related obesity. To combat obesity and its progression, the current study is focusing on the benefits of adipokines as a new trend biomarker for detecting obesity updates. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
13. A comparative assessment of reference genes in mouse brown adipocyte differentiation and thermogenesis in vitro
- Author
-
Trang Huyen Lai, Jin Seok Hwang, Quang Nhat Ngo, Dong-Kun Lee, Hyun Joon Kim, and Deok Ryong Kim
- Subjects
Reference gene ,brown adipocytes ,Ucp1 ,thermogenesis ,adipocyte differentiation ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Cytology ,QH573-671 ,Physiology ,QP1-981 - Abstract
ABSTRACTAdipogenic differentiation and thermogenesis in brown adipose tissue (BAT) undergo dynamic processes, altering phenotypes and gene expressions. Proper reference genes in gene expression analysis are crucial to mitigate experimental variances and ensure PCR efficacy. Unreliable reference genes can lead to erroneous gene expression quantification, resulting in data misinterpretation. This study focused on identifying suitable reference genes for mouse brown adipocyte research, utilizing brown adipocytes from the Ucp1-luciferase ThermoMouse model. Comparative analysis of gene expression data under adipogenesis and thermogenesis conditions was conducted, validating 13 housekeeping genes through various algorithms, including DeltaCq, BestKeeper, geNorm, Normfinder, and RefFinder. Tbp and Rer1 emerged as optimal references for Ucp1 and Pparg expression in brown adipogenesis, while Tbp and Ubc were ideal for the expression analysis of these target genes in thermogenesis. Conversely, certain conventional references, including Actb, Tubb5, and Gapdh, proved unstable as reference genes under both conditions. These findings stress the critical consideration of reference gene selection in gene expression analysis within specific biological systems to ensure accurate conclusions.
- Published
- 2024
- Full Text
- View/download PDF
14. Ca2+ Depletion in the ER Causes Store-Operated Ca2+ Entry via the TRPC6 Channel in Mouse Brown Adipocytes.
- Author
-
Ryotaro HAYATO, Takaya MATSUMOTO, and Yoko HIGURE
- Subjects
SYMPATHOMIMETIC agents ,FAT cells ,ENDOPLASMIC reticulum ,INTRACELLULAR membranes ,FLUORIMETRY - Abstract
ß3-adrenergic activation causes Ca2+ release from the mitochondria and subsequent Ca2+ release from the endoplasmic reticulum (ER), evoking store-operated Ca2+ entry (SOCE) due to Ca2+ depletion from the ER in mouse brown adipocytes. In this study, we investigated how Ca2+ depletion from the ER elicits SOCE in mouse brown adipocytes using fluorometry of intracellular Ca2+ concentration ([Ca2+]i). The administration of cyclopiazonic acid (CPA), a reversible sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) pump blocker in the ER, caused an increase in [Ca2+]i. Moreover, CPA-induced SOCE was suppressed by the administration of a Ca2+-free Krebs solution and the transient receptor potential canonical 6 (TRPC6) selective blockers 2-APB, ML-9 and GsMTx-4 but not Pico145, which blocks TRPC1/4/5. Administration of TRPC6 channel agonist 1-oleoyl-2-acetyl-sn-glycerol (OAG) and flufenamic acid elicited Ca2+ entry. Moreover, our RT-PCR analyses detected mRNAs for TRPC6 in brown adipose tissues. In addition, western blot analyses showed the expression of the TRPC6 protein. Thus, TRPC6 is one of the Ca2+ pathways involved in SOCE. These modes of Ca2+ entry provide the basis for heat production via activation of Ca2+-dependent dehydrogenase and the expression of uncoupling protein 1 (UCP1). Enhancing thermogenic metabolism in brown adipocytes may serve as broad therapeutic utility to reduce obesity and metabolic syndrome. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
15. 双硫仑治疗小鼠肥胖的安全性和有效性评价.
- Author
-
武晓慧, 马子怡, 张钊华, 张琳贻, 党怡雄, and 廉 婷
- Abstract
Objective: To observe the safety and efficacy of disulfiram in the treatment of obesity in mice. Methods: Ten 6-week-old C57BL/6J male mice were randomly divided into disulfiram group (disulfiram dissolved in corn oil, 300 mg/ (kg·d) and control group (equivalent amount of corn oil), with 5 mice in each group. The mice were administered once a day by intragastric administration for 2 weeks, during which high-fat diet was still given. Food consumption and body weight of the mice were monitored during the disulfiram administration. Serum, white adipose pad of epididymis, brown adipose tissue in the scapular area, and liver were taken after administration. White and brown adipose tissue, and liver were stained with HE, and the cell morphology was observed. Lipid droplets and mitochondria in brown adipocytes were observed under electron microscopy. Realtime-qPCR was used to detect the relative m RNA expression levels of Ucp1, Fabp4, Prdml6, and Cidea in brown adipose tissue, while Western blot was used to detect the protein expression level of Ucp1. Levels of aminotransferase ALT and AST in serum was also detected. Ten C57BL/6J male mice aged 8 weeks were randomly divided into disulfiram group (disulfiram 300 mg/ (kg·d) and control group (equivalent amount of corn oil). They were intragastrically administered once a day for two weeks. After administration, HE staining of the brown adipose tissue and liver were performed, and ALT and AST contents in serum were detected. Ten C57BL/6J male mice aged 8 weeks were randomly divided into disulfiram group (disulfiram 300 mg/ (kg·d) and control group (equivalent amount of corn oil). They were intragastically administered once a day for four weeks, and HE staining of the liver was performed. Serum levels of ALT and AST were measured. C57BL/6J embryonic mice with a gestational age of 13.5 days were selected for primary fibroblast culture. They were divided into disulfiram group (5 mg/L disulfiram) and control group (equivalent amount of DMSO) and induced to differentiate into brown adipocytes. After 8 days of differentiation, oil red O staining was performed to observe the formation of lipid droplets, and the relative m RNA and protein expression levels of Ucp1, Fabp4, Prdml6, and Cidea were detected. Results: During the administration, there was no significant difference in food intake and weight changes between the disulfiram group and the control group (P>0.05). After administration, there was no significant difference in the size of white adipocytes between the two groups. The diameter of brown adipocytes and intracellular lipid droplets in the disulfiram group of mice were significantly increased (P<0.05), but there was no significant difference in the number of lipid droplets, mitochondrial morphology and mitochondrial number (P>0.05). The m RNA expression of Cidea and Prdm16 in brown adipose tissue of mice in the disulfiram group were decreased (P<0.05). For mice with normal weight, after 2 weeks of administration of disulfiram, the lipid droplets of brown adipocytes were also enlarged. The results of cell experiments showed that the formation of lipid droplets was significantly reduced in the disulfiram group, and the m RNA expression of Ucp1, Cidea, and Prdm16 was significantly reduced (P<0.05); The protein expression of Ucp1 was significantly reduced (P<0.05). Both obese and normal weight mice showed significant liver cell edema after 2 weeks of administration of disulfiram, with elevated levels of ALT and AST in serum (P<0.05). Normal weight mice also showed significant liver cell edema after 4 weeks of disulfiram administration, with elevated levels of ALT and AST (P<0.05). Conclusion: Short term use of disulfiram has no significant effect on weight loss of diet-induced obese mice; disulfiram can inhibit the differentiation of mice’s brown adipocytes both in vivo and in vitro. Short-term use of disulfiram can cause liver damage. The safety and effectiveness of disulfiram in weight loss treatment are not ideal enough. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
16. Inosine: novel activator of brown adipose tissue and energy homeostasis.
- Author
-
Pfeifer, Alexander, Mikhael, Mickel, and Niemann, Birte
- Subjects
- *
BROWN adipose tissue , *PURINERGIC receptors , *INOSINE , *NUCLEOSIDE transport proteins , *BODY mass index , *FAT cells , *HOMEOSTASIS - Abstract
Inosine is released from brown adipocytes (BAs) upon stress. Inosine activates purinergic P1 receptors, triggering BA activation and white adipocyte browning. Consequently, inosine increases whole-body energy consumption and alleviates diet-induced obesity in mice. The equilibrative nucleoside transporter 1 (ENT1) transports extracellular inosine into cells. By inhibiting ENT1 using dipyridamole, extracellular inosine levels are elevated. A loss-of-function mutation of ENT1 in humans is associated with a reduced body mass index in the carriers. Enhancing inosine concentrations might have therapeutic potential to tackle obesity. Extracellular purinergic molecules act as signaling molecules that bind to cellular receptors and regulate signaling pathways. Growing evidence suggests that purines regulate adipocyte function and whole-body metabolism. Here, we focus on one specific purine: inosine. Brown adipocytes, which are important regulators of whole-body energy expenditure (EE), release inosine when they are stressed or become apoptotic. Unexpectedly, inosine activates EE in neighboring brown adipocytes and enhances differentiation of brown preadipocytes. Increasing extracellular inosine, either directly by increasing inosine intake or indirectly via pharmacological inhibition of cellular inosine transporters, increases whole-body EE and counteracts obesity. Thus, inosine and other closely related purines might be a novel approach to tackle obesity and associated metabolic disorders by enhancing EE. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. Platelet-derived thrombospondin 1 promotes immune cell liver infiltration and exacerbates diet-induced steatohepatitis
- Author
-
Taesik Gwag, Sangderk Lee, Zhenyu Li, Alana Newcomb, Josephine Otuagomah, Steven A. Weinman, Ying Liang, Changcheng Zhou, and Shuxia Wang
- Subjects
Platelet α granule ,TSP1 ,NASH ,brown adipocytes ,organ crosstalk ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background & Aims: Recent studies have implicated platelets, particularly α-granules, in the development of non-alcoholic steatohepatitis (NASH). However, the specific mechanisms involved have yet to be determined. Notably, thrombospondin 1 (TSP1) is a major component of the platelet α-granules released during platelet activation. Hence, we aimed to determine the role of platelet-derived TSP1 in NASH. Methods: Platelet-specific Tsp1 knockout mice (TSP1Δpf4) and their wild-type littermates (TSP1F/F) were used. NASH was induced by feeding the mice with a diet enriched in fat, sucrose, fructose, and cholesterol (AMLN diet). A human liver NASH organoid model was also employed. Results: Although TSP1 deletion in platelets did not affect diet-induced steatosis, TSP1Δpf4 mice exhibited attenuated NASH and liver fibrosis, accompanied by improvements in plasma glucose and lipid homeostasis. Furthermore, TSP1Δpf4 mice showed reduced intrahepatic platelet accumulation, activation, and chemokine production, correlating with decreased immune cell infiltration into the liver. Consequently, this diminished proinflammatory signaling in the liver, thereby mitigating the progression of NAFLD. Moreover, in vitro data revealed that co-culturing TSP1-deficient platelets in a human liver NASH organoid model attenuated hepatic stellate cell activation and NASH progression. Additionally, TSP1-deficient platelets play a role in regulating brown fat endocrine function, specifically affecting Nrg4 (neuregulin 4) production. Crosstalk between brown fat and the liver may also influence the progression of NAFLD. Conclusions: These data suggest that platelet α-granule-derived TSP1 is a significant contributor to diet-induced NASH and fibrosis, potentially serving as a new therapeutic target for this severe liver disease. Impact and implications: Recent studies have implicated platelets, specifically α-granules, in the development of non-alcoholic steatohepatitis, yet the precise mechanisms remain unknown. In this study, through the utilization of a tissue-specific knockout mouse model and human 3D liver organoid, we demonstrated that platelet α-granule-derived TSP1 significantly contributes to diet-induced non-alcoholic steatohepatitis and fibrosis. This contribution is, in part, attributed to the regulation of intrahepatic immune cell infiltration and potential crosstalk between fat and the liver. These findings suggest that platelet-derived TSP1 may represent a novel therapeutic target in non-alcoholic fatty liver disease.
- Published
- 2024
- Full Text
- View/download PDF
18. Transcriptomics profiling reveal the heterogeneity of white and brown adipocyte.
- Author
-
Zhang, Zhongxiao, Xu, Liling, Zhang, Ling, Lu, Jingxian, Peng, Zhou, Guo, Xirong, and Gao, Jianfang
- Subjects
- *
ADIPOGENESIS , *FAT cells , *FOCAL adhesions , *OXIDATIVE phosphorylation , *HETEROGENEITY , *SOX transcription factors - Abstract
The marker genes associated with white adipocytes and brown adipocytes have been previously identified; however, these markers have not been updated in several years, and the differentiation process of preadipocytes remains relatively fixed. Consequently, there has been a lack of exploration into alternative differentiation schemes. In this particular study, we present a transcriptional signature specific to brown adipocytes and white adipocytes. Notably, our findings reveal that ZNF497, ZIC1, ZFY, UTY, USP9Y, TXLNGY, TTTY14, TNNT3, TNNT2, TNNT1, TNNI1, TNNC1, TDRD15, SOX11, SLN, SFRP2, PRKY, PAX3KLHL40, PAX3, INKA2-AS1, SOX11, and TDRD15 exhibit high expression levels in brown adipocytes. XIST, HOXA10, PCAT19, HOXA7, PLSCR3, and AVPR1A exhibited high expression levels in white adipocytes, suggesting their potential as novel marker genes for the transition from white to brown adipocytes. Furthermore, our analysis revealed the coordinated activation of several pathways, including the PPAR signaling pathway, focal adhesion, retrograde endocannabinoid signaling, oxidative phosphorylation, PI3K-Akt signaling pathway, and thermogenesis pathways, in brown adipocytes. Moreover, in contrast to prevailing culture techniques, we conducted a comparative analysis of the differentiation protocols for white preadipocytes and brown preadipocytes, revealing that the differentiation outcome remained unaffected by the diverse culture schemes employed. However, the expression levels of certain marker genes in both adipocyte types were found to be altered. This investigation not only identified potential novel marker genes for adipocytes but also examined the impact of different differentiation methods on preadipocyte maturation. Consequently, these findings offer significant insights for further research on the differentiation processes of diverse adipocyte subtypes. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
19. CTRP9 Promotes Brown Adipose Tissue Lipolysis in Mice Fed a High-Fat Diet
- Author
-
Hua Guan, Le Wang, Zhanyi Geng, Bowen Duan, Yang Gao, Zheyong Liang, Xinglong Zheng, Tao Shi, and Fengwei Guo
- Subjects
ctrp9 ,ucp-1 ,atgl ,lipolysis ,brown adipocytes ,Biochemistry ,QD415-436 ,Biology (General) ,QH301-705.5 - Abstract
Background: This study aimed to elucidate the molecular mechanism through which C1q/tumor necrosis factor (TNF)-related protein 9 (CTRP9) acts in the formation and differentiation of brown adipose tissue (BAT). Methods: Adenovirus particles encoding CTRP9 and green fluorescent protein were inoculated into the scapula of C57BL/6J mice and fed a high-fat diet for 8 weeks; the body weight, lipid droplet morphology, glucose tolerance, insulin tolerance, and protein expression levels were analyzed. In addition, CTRP9 adenovirus was transfected into brown preadipocytes, and differentiation was induced to identify the effect of CTRP9 overexpression on adipocyte differentiation. Results: CTRP9 overexpression significantly increased the weight gain of mice. Additionally, the CTRP9 overexpression group exhibited significantly increased adipose tissue weight and glucose clearance rates and decreased insulin sensitivity and serum triglyceride levels compared to the control group. Furthermore, CTRP9 overexpression significantly upregulated the adipose triglyceride lipase (ATGL) and perilipin 1 protein expression levels in BAT. The cell experiment results confirmed that CTRP9 overexpression significantly inhibited the adipogenesis of brown adipocytes as evidenced by the downregulation of uncoupling protein 1, beta-3 adrenergic receptor, ATGL, and hormone-sensitive lipase mRNA levels and the significant suppression of uncoupling protein 1, ATGL, and perilipin 1 protein levels in brown adipocytes. Conclusions: The finding of this study demonstrated that CTRP9 promotes lipolysis by upregulating ATGL expression in vivo and inhibits the differentiation of brown preadipocytes in vitro.
- Published
- 2024
- Full Text
- View/download PDF
20. Silicon dioxide nanoparticles inhibit the effects of cold exposure on metabolism and inflammatory responses in brown adipocytes
- Author
-
Zhang Yongqiang, Zhang Li, Wu Shuai, Zhang Guanyu, Wei Xiaodie, Li Xi, and Yang Danfeng
- Subjects
sio2 nanoparticles ,cold exposure ,metabolic ,inflammatory response ,brown adipocytes ,Special situations and conditions ,RC952-1245 - Abstract
Nanoparticles (NPs) in haze are potentially hazardous to health, which is more severe in the winter. Brown adipose tissue (BAT) plays important roles in obesity, insulin resistance, and diabetes. Though the toxicology of NPs has been intensively studied, few studies have been reported on the antagonistic effects between Silicon dioxide(SiO2) NPs and cold exposure in brown adipocytes.
- Published
- 2023
- Full Text
- View/download PDF
21. Functional roles of pantothenic acid, riboflavin, thiamine, and choline in adipocyte browning in chemically induced human brown adipocytes
- Author
-
Takeda, Yukimasa and Dai, Ping
- Published
- 2024
- Full Text
- View/download PDF
22. Mitochondrial respiratory complex I deficiency inhibits brown adipogenesis by limiting heme regulation of histone demethylation.
- Author
-
Liu, Jingjing, Lu, Wen, Yan, Dongyue, Guo, Junyuan, Zhou, Li, Shi, Bimin, and Su, Xiong
- Subjects
- *
ADIPOGENESIS , *HEME , *KREBS cycle , *DEMETHYLATION , *MITOCHONDRIA , *HISTONE methylation , *FAT cells - Abstract
[Display omitted] • OXPHOS deficiency limits TCA cycle intermediates and reduces de novo heme synthesis. • Hemin partially rescues the inhibited brown adipogenesis by OXPHOS deficiency. • Hemin regulates adipogenic transcription potentially via histone methylation. Mitochondrial functions play a crucial role in determining the metabolic and thermogenic status of brown adipocytes. Increasing evidence reveals that the mitochondrial oxidative phosphorylation (OXPHOS) system plays an important role in brown adipogenesis, but the mechanistic insights are limited. Herein, we explored the potential metabolic mechanisms leading to OXPHOS regulation of brown adipogenesis in pharmacological and genetic models of mitochondrial respiratory complex I deficiency. OXPHOS deficiency inhibits brown adipogenesis through disruption of the brown adipogenic transcription circuit without affecting ATP levels. Neither blockage of calcium signaling nor antioxidant treatment can rescue the suppressed brown adipogenesis. Metabolomics analysis revealed a decrease in levels of tricarboxylic acid cycle intermediates and heme. Heme supplementation specifically enhances respiratory complex I activity without affecting complex II and partially reverses the inhibited brown adipogenesis by OXPHOS deficiency. Moreover, the regulation of brown adipogenesis by the OXPHOS-heme axis may be due to the suppressed histone methylation status by increasing histone demethylation. In summary, our findings identified a heme-sensing retrograde signaling pathway that connects mitochondrial OXPHOS to the regulation of brown adipocyte differentiation and metabolic functions. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
23. The Role of Proton-Coupled Amino Acid Transporter 2 (SLC36A2) in Cold-Induced Thermogenesis of Mice.
- Author
-
Shu, Hui, Zhang, Jie, Cheng, Dawei, Zhao, Xiaorui, Ma, Yue, Zhang, Chi, Zhang, Yong, Jia, Zhihao, and Liu, Zhiwei
- Abstract
Brown adipocytes mainly utilize glucose and fatty acids to produce energy, which play key roles in thermogenesis. Furthermore, brown adipocytes also utilize other substrates, such as amino acids, for energy expenditure in various conditions. Here, we report the new physiological roles of proton-coupled amino acid transporters, SLC36A2 and SLC36A3, on global energy metabolism. The relative mRNA expression levels of both Slc36a2 and Slc36a3 were all highest in brown adipose tissue. We then generated global Slc36a2 and Slc36a3 knockout mice to investigate their functions in metabolism. Neither loss of Slc36a2 nor Slc36a3 affected the body weight and body composition of the mice. Slc36a2 knockout mice exhibited increased oxygen consumption during the daytime. After cold treatment, inhibition of Slc36a2 significantly decreased the mass of brown adipose tissue compared to wildtype mice, while it lowered the expression level of Cpt1a. Moreover, the serum lipid levels and liver mass were also decreased in Slc36a2 knockout mice after cold treatment. On the contrary, Slc36a3 knockout impaired glucose tolerance and up-regulated serum LDL-cholesterol concentration. Thus, SLC36A2 and SLC36A3 play central and different roles in the energy metabolism of the mice. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
24. Editorial: Novel regulatory mechanisms behind thermogenesis of brown and beige adipocytes
- Author
-
Abhirup Shaw, Endre Kristóf, and Rubén Cereijo
- Subjects
obesity ,brown adipocytes ,beige adipocytes ,UCP1 ,thermogenesis ,beiging ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Published
- 2023
- Full Text
- View/download PDF
25. The role of proteasome activators PA28αβ and PA200 in brown adipocyte differentiation and function.
- Author
-
Koçberber, Zeynep, Willemsen, Nienke, and Bartelt, Alexander
- Subjects
FAT cells ,BROWN adipose tissue ,GENE expression - Abstract
Introduction: Brown adipocytes produce heat through non shivering thermogenesis (NST). To adapt to temperature cues, they possess a remarkably dynamic metabolism and undergo substantial cellular remodeling. The proteasome plays a central role in proteostasis and adaptive proteasome activity is required for sustained NST. Proteasome activators (PAs) are a class of proteasome regulators but the role of PAs in brown adipocytes is unknown. Here, we studied the roles of PA28α (encoded by Psme1) and PA200 (encoded by Psme4) in brown adipocyte differentiation and function. Methods: We measured gene expression in mouse brown adipose tissue. In cultured brown adipocytes, we silenced Psme1 and/or Psme4 expression through siRNA transfection. We then assessed impact on the ubiquitin proteasome system, brown adipocyte differentiation and function. Results: We found that Psme1 and Psme4 are expressed in brown adipocytes in vivo and in vitro. Through silencing of Psme1 and/or Psme4 expression in cultured brown adipocytes, we found that loss of PAs did not impair proteasome assembly or activity, and that PAs were not required for proteostasis in this model. Loss of Psme1 and/or Psme4 did not impair brown adipocyte development or activation, suggesting that PAs are neither required for brown adipogenesis nor NST. Discussion: In summary, we found no role for Psme1 and Psme4 in brown adipocyte proteostasis, differentiation, or function. These findings contribute to our basic understanding of proteasome biology and the roles of proteasome activators in brown adipocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
26. Berberine Ameliorates Obesity by Inducing GDF15 Secretion by Brown Adipocytes.
- Author
-
Li, Chang, Leng, Qingyang, Li, Lihua, Hu, Fan, Xu, Yuejie, Gong, Sa, Yang, Ying, Zhang, Hongli, and Li, Xiaohua
- Subjects
BERBERINE ,OBESITY ,FAT cells - Abstract
Berberine (BBR), which is a compound derived from the Chinese medicinal plant Coptis chinensis , promotes weight loss, but the molecular mechanisms are not well understood. Here, we show that BBR increases the serum level of growth differentiation factor 15 (GDF15), which is a stress response cytokine that can reduce food intake and lower body weight in diet-induced obese (DIO) mice. The body weight and food intake of DIO mice were decreased after BBR treatment, and the weight change was negatively correlated with the serum GDF15 level. Further studies show that BBR induced GDF15 mRNA expression and secretion in the brown adipose tissue (BAT) of DIO mice and primary mouse brown adipocytes. In addition, we found that BBR upregulates GDF15 mRNA expression and secretion by activating the integrated stress response (ISR) in primary mouse brown adipocytes. Overall, our findings show that BBR lowers body weight by inducing GDF15 secretion via the activation of the ISR in BAT. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
27. Brown adipocytes promote epithelial mesenchymal transition of neuroblastoma cells by inducing PPAR-γ/UCP2 expression
- Author
-
Zhijuan Ge, Yue Shang, Wendie Wang, Jigang Yang, and Shu-Zhen Chen
- Subjects
Brown adipocytes ,neuroblastoma ,genipin ,uncoupling protein ,PPAR-γ ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 ,Cytology ,QH573-671 ,Physiology ,QP1-981 - Abstract
Neuroblastoma (NB) is an embryonic malignant tumour of the sympathetic nervous system, and current research shows that activation of brown adipose tissue accelerates cachexia in cancer patients. However, the interaction between brown adipose tissues and NB remains unclear. The study aimed to investigate the effect of brown adipocytes in the co-culture system on the proliferation and migration of NB cells. Brown adipocytes promoted the proliferation and migration of Neuro-2a, BE(2)-M17, and SH-SY5Y cells under the co-culture system, with an increase of the mRNA and protein levels of UCP2 and PPAR-γ in NB cells. The UCP2 inhibitor genipin or PPAR-γ inhibitor T0090709 inhibited the migration of NB cells induced by brown adipocytes. Genipin or siUCP2 upregulated the expression of E-cadherin, and downregulated the expression of N-cadherin and vimentin in NB cells. We suggest that under co-cultivation conditions, NB cells can activate brown adipocytes, which triggers changes in various genes and promotes the proliferation and migration of NB cells. The PPAR-γ/UCP2 pathway is involved in the migration of NB cells caused by brown adipocytes.
- Published
- 2022
- Full Text
- View/download PDF
28. The Role of Thermogenic Fat Tissue in Energy Consumption
- Author
-
Masato Horino, Kenji Ikeda, and Tetsuya Yamada
- Subjects
non-shivering thermogenesis ,thermogenic fat tissue ,uncoupling protein 1 ,beige adipocytes ,brown adipocytes ,glucose metabolism ,Biology (General) ,QH301-705.5 - Abstract
Mammalian adipose tissues are broadly divided into white adipose tissue (WAT) and thermogenic fat tissue (brown adipose tissue and beige adipose tissue). Uncoupling protein 1 (UCP1) is the central protein in thermogenesis, and cells that exhibit induced UCP1 expression and appear scattered throughout WAT are called beige adipocytes, and their induction in WAT is referred to as “beiging”. Beige adipocytes can differentiate from preadipocytes or convert from mature adipocytes. UCP1 was thought to contribute to non-shivering thermogenesis; however, recent studies demonstrated the presence of UCP1-independent thermogenic mechanisms. There is evidence that thermogenic fat tissue contributes to systemic energy expenditure even in human beings. This review discusses the roles that thermogenic fat tissue plays in energy consumption and offers insight into the possibility and challenges associated with its application in the treatment of obesity and type 2 diabetes.
- Published
- 2022
- Full Text
- View/download PDF
29. Novel Therapeutic Potentials of Taxifolin for Obesity-Induced Hepatic Steatosis, Fibrogenesis, and Tumorigenesis.
- Author
-
Inoue, Takayuki, Fu, Bin, Nishio, Miwako, Tanaka, Miyako, Kato, Hisashi, Tanaka, Masashi, Itoh, Michiko, Yamakage, Hajime, Ochi, Kozue, Ito, Ayaka, Shiraki, Yukihiro, Saito, Satoshi, Ihara, Masafumi, Nishimura, Hideo, Kawamoto, Atsuhiko, Inoue, Shian, Saeki, Kumiko, Enomoto, Atsushi, Suganami, Takayoshi, and Satoh-Asahara, Noriko
- Abstract
The molecular pathogenesis of nonalcoholic steatohepatitis (NASH) includes a complex interaction of metabolic stress and inflammatory stimuli. Considering the therapeutic goals of NASH, it is important to determine whether the treatment can prevent the progression from NASH to hepatocellular carcinoma. Taxifolin, also known as dihydroquercetin, is a natural bioactive flavonoid with antioxidant and anti-inflammatory properties commonly found in various foods and health supplement products. In this study, we demonstrated that Taxifolin treatment markedly prevented the development of hepatic steatosis, chronic inflammation, and liver fibrosis in a murine model of NASH. Its mechanisms include a direct action on hepatocytes to inhibit lipid accumulation. Taxifolin also increased brown adipose tissue activity and suppressed body weight gain through at least two distinct pathways: direct action on brown adipocytes and indirect action via fibroblast growth factor 21 production in the liver. Notably, the Taxifolin treatment after NASH development could effectively prevent the development of liver tumors. Collectively, this study provides evidence that Taxifolin shows pleiotropic effects for the treatment of the NASH continuum. Our data also provide insight into the novel mechanisms of action of Taxifolin, which has been widely used as a health supplement with high safety. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
30. Regulatory role of atrial natriuretic peptide in brown adipose tissue: A narrative review.
- Author
-
Lu, Guanhua, Hu, Ruixiang, Tao, Tian, Hu, Min, Dong, Zhiyong, and Wang, Cunchuan
- Subjects
- *
ATRIAL natriuretic peptides , *BROWN adipose tissue , *HORMONE regulation , *PEPTIDE receptors , *HEART metabolism , *BRAIN natriuretic factor - Abstract
Summary: Atrial natriuretic peptide (ANP) has been considered to exert an essential role as a cardiac secretory hormone in the regulation of hemodynamic homeostasis. As the research progresses, the role of ANP in the crosstalk between heart and lipid metabolism has become an interesting topic that is attracting the interest of researchers. The regulation of ANP in lipid metabolism shows favorable effects, particularly the activation of brown adipose tissue (BAT). The complex regulatory network of ANP on BAT has not been fully outlined. This narrative review critically evaluated the existing literature on the regulatory effects of ANP on BAT. In general, we have summarized the expression of ANP and its receptors in various human tissues, analyzed the progress of research on the relationship between the ANP and BAT, and described several potential pathways of ANP to BAT. Exogenous ANP, natriuretic peptide receptor C (NPRC) deficiency, cold exposure, bariatric surgery, and cardiac or renal insufficiency could all contribute to BAT expression by increasing circulating ANP levels. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
31. ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats
- Author
-
Marija Aleksic, Igor Golic, Aleksandra Jankovic, Aleksandra Cvoro, and Aleksandra Korac
- Subjects
hypothyroidism ,brown adipocytes ,peroxisomes ,ACOX1 ,ACOX3 ,Science - Abstract
We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.
- Published
- 2023
- Full Text
- View/download PDF
32. The role of proteasome activators PA28αβ and PA200 in brown adipocyte differentiation and function
- Author
-
Zeynep Koçberber, Nienke Willemsen, and Alexander Bartelt
- Subjects
BAT ,brown adipocytes ,proteostasis ,ubiquitin-proteasome-system ,PA28αβ ,PA200 ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
IntroductionBrown adipocytes produce heat through non shivering thermogenesis (NST). To adapt to temperature cues, they possess a remarkably dynamic metabolism and undergo substantial cellular remodeling. The proteasome plays a central role in proteostasis and adaptive proteasome activity is required for sustained NST. Proteasome activators (PAs) are a class of proteasome regulators but the role of PAs in brown adipocytes is unknown. Here, we studied the roles of PA28α (encoded by Psme1) and PA200 (encoded by Psme4) in brown adipocyte differentiation and function.MethodsWe measured gene expression in mouse brown adipose tissue. In cultured brown adipocytes, we silenced Psme1 and/or Psme4 expression through siRNA transfection. We then assessed impact on the ubiquitin proteasome system, brown adipocyte differentiation and function.ResultsWe found that Psme1 and Psme4 are expressed in brown adipocytes in vivo and in vitro. Through silencing of Psme1 and/or Psme4 expression in cultured brown adipocytes, we found that loss of PAs did not impair proteasome assembly or activity, and that PAs were not required for proteostasis in this model. Loss of Psme1 and/or Psme4 did not impair brown adipocyte development or activation, suggesting that PAs are neither required for brown adipogenesis nor NST.DiscussionIn summary, we found no role for Psme1 and Psme4 in brown adipocyte proteostasis, differentiation, or function. These findings contribute to our basic understanding of proteasome biology and the roles of proteasome activators in brown adipocytes.
- Published
- 2023
- Full Text
- View/download PDF
33. Stress-inducible IL-6 is regulated by KLF7 in brown adipocytes
- Author
-
Yihan Tang, Dingling Ma, Maodi Liang, Yanting Hou, Meixiu Zhang, Jingzhou Wang, Chenggang Yuan, Menghuan Li, Chaoyue Sun, Jianxin Xie, Cuizhe Wang, and Jun Zhang
- Subjects
ADRB3 ,Brown adipocytes ,KLF7 ,IL-6 ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Stress-inducible interleukin 6 (IL-6) is generated in brown adipocytes via beta-3 adrenergic receptor (ADRB3) signaling, which is necessary in stress hyperglycemia, the kind of metabolic adaptation enabling “fight or flight” response by means of liver gluconeogenesis. Nevertheless, the mechanism of ADRB3 signaling mediates IL-6 in brown adipocytes remains unclear. As a result, it is critical to understand how brown adipocytes produce IL-6 via ADRB3 signaling. We found that the ADRB3 agonist and cold stimulation promoted the expression of KLF7 and IL-6 in brown adipocytes of mice. In parallel to these results in vivo, treatment with ADRB3 agonist promoted the expression of KLF7 and the release of IL-6 in primary brown adipocytes of mice. Notably, we discovered that KLF7 positively controls the expression of IL-6 and downregulated KLF7 largely blunted ADRB3 agonist induced IL-6 expressions in brown adipocytes. Our findings suggest that KLF7 is required for the generation of IL-6 when ADRB3 signaling is activated in brown adipocytes.
- Published
- 2023
- Full Text
- View/download PDF
34. Editorial: Novel regulatory mechanisms behind thermogenesis of brown and beige adipocytes.
- Author
-
Shaw, Abhirup, Kristóf, Endre, and Cereijo, Rubén
- Subjects
BODY temperature regulation ,FAT cells - Published
- 2023
- Full Text
- View/download PDF
35. The role of DNA methylation in thermogenic adipose biology
- Author
-
Xiao, Han and Kang, Sona
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Obesity ,Diabetes ,Nutrition ,Genetics ,Underpinning research ,1.1 Normal biological development and functioning ,Adipose Tissue ,Beige ,Adipose Tissue ,Brown ,Animals ,DNA Methylation ,Epigenesis ,Genetic ,Gene Expression Regulation ,Gene Regulatory Networks ,Humans ,Thermogenesis ,Epigenetics ,DNA methylation ,brown adipocytes ,beige adipocytes ,obesity ,type 2 diabetes ,Medical Biochemistry and Metabolomics ,Developmental Biology ,Biochemistry and cell biology - Abstract
The two types of thermogenic fat cells, beige and brown adipocytes, play a significant role in regulating energy homeostasis. Their development and thermogenesis are tightly regulated by dynamic epigenetic mechanisms, which could potentially be targeted to treat metabolic disorders such as obesity. However, we are just beginning to catalog and understand these dynamic changes. In this review, we will discuss the current understanding of the role of DNA (de)methylation events in beige and brown adipose biology in order to highlight the holes in our knowledge and to point the way forward for future studies.
- Published
- 2019
36. Recent insights into the molecular mechanisms of simultaneous fatty acid oxidation and synthesis in brown adipocytes
- Author
-
Ji Suk Chang
- Subjects
brown adipocytes ,fatty acid oxidation ,de novo fatty acid synthesis ,mitochondrial substrate utilization ,uncoupled respiration ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Brown adipocytes is a specialized fat cell that dissipates nutrient-derived chemical energy in the form of heat, instead of ATP synthesis. This unique feature provides a marked capacity for brown adipocyte mitochondria to oxidize substrates independent of ADP availability. Upon cold exposure, brown adipocytes preferentially oxidize free fatty acids (FFA) liberated from triacylglycerol (TAG) in lipid droplets to support thermogenesis. In addition, brown adipocytes take up large amounts of circulating glucose, concurrently increasing glycolysis and de novo FA synthesis from glucose. Given that FA oxidation and glucose-derived FA synthesis are two antagonistic mitochondrial processes in the same cell, it has long been questioned how brown adipocytes run FA oxidation and FA synthesis simultaneously. In this review, I summarize mechanisms regulating mitochondrial substrate selection and describe recent findings of two distinct populations of brown adipocyte mitochondria with different substrate preferences. I further discuss how these mechanisms may permit a concurrent increase in glycolysis, FA synthesis, and FA oxidation in brown adipocytes.
- Published
- 2023
- Full Text
- View/download PDF
37. Brown adipocytes promote epithelial mesenchymal transition of neuroblastoma cells by inducing PPAR-γ/UCP2 expression.
- Author
-
Ge, Zhijuan, Shang, Yue, Wang, Wendie, Yang, Jigang, and Chen, Shu-Zhen
- Subjects
- *
EPITHELIAL-mesenchymal transition , *FAT cells , *BROWN adipose tissue , *CELL migration , *SYMPATHETIC nervous system - Abstract
Neuroblastoma (NB) is an embryonic malignant tumour of the sympathetic nervous system, and current research shows that activation of brown adipose tissue accelerates cachexia in cancer patients. However, the interaction between brown adipose tissues and NB remains unclear. The study aimed to investigate the effect of brown adipocytes in the co-culture system on the proliferation and migration of NB cells. Brown adipocytes promoted the proliferation and migration of Neuro-2a, BE(2)-M17, and SH-SY5Y cells under the co-culture system, with an increase of the mRNA and protein levels of UCP2 and PPAR-γ in NB cells. The UCP2 inhibitor genipin or PPAR-γ inhibitor T0090709 inhibited the migration of NB cells induced by brown adipocytes. Genipin or siUCP2 upregulated the expression of E-cadherin, and downregulated the expression of N-cadherin and vimentin in NB cells. We suggest that under co-cultivation conditions, NB cells can activate brown adipocytes, which triggers changes in various genes and promotes the proliferation and migration of NB cells. The PPAR-γ/UCP2 pathway is involved in the migration of NB cells caused by brown adipocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
38. Transcriptomic profiling of the telomerase transformed Mesenchymal stromal cells derived adipocytes in response to rosiglitazone.
- Author
-
Al-Ali, Moza Mohamed, Khan, Amir Ali, Fayyad, Abeer Maher, Abdallah, Sallam Hasan, and Khattak, Muhammad Nasir Khan
- Abstract
Background: Differentiation of Immortalized Human Bone Marrow Mesenchymal Stromal Cells - hTERT (iMSC3) into adipocytes is in vitro model of obesity. In our earlier study, rosiglitazone enhanced adipogenesis particularly the brown adipogenesis of iMSC3. In this study, the transcriptomic profiles of iMSC3 derived adipocytes with and without rosiglitazone were analyzed through mRNA sequencing. Results: A total of 1508 genes were differentially expressed between iMSC3 and the derived adipocytes without rosiglitazone treatment. GO and KEGG enrichment analyses revealed that rosiglitazone regulates PPAR and PI3K-Akt pathways. The constant rosiglitazone treatment enhanced the expression of Fatty Acid Binding Protein 4 (FABP4) which enriched GO terms such as fatty acid binding, lipid droplet, as well as white and brown fat cell differentiation. Moreover, the constant treatment upregulated several lipid droplets (LDs) associated proteins such as PLIN1. Rosiglitazone also activated the receptor complex PTK2B that has essential roles in beige adipocytes thermogenic program. Several uniquely expressed novel regulators of brown adipogenesis were also expressed in adipocytes derived with rosiglitazone: PRDM16, ZBTB16, HOXA4, and KLF15 in addition to other uniquely expressed genes. Conclusions: Rosiglitazone regulated several differentially regulated genes and non-coding RNAs that warrant further investigation about their roles in adipogenesis particularly brown adipogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
39. FAM96A is essential for maintaining organismal energy balance and adipose tissue homeostasis in mice.
- Author
-
Liu, Zhuanzhuan, Xu, Shihong, Zhang, Zhiwei, Wang, Hanying, Jing, Qiyue, Zhang, Shenghan, Liu, Mengnan, Han, Jinzhi, Kou, Yanbo, Wei, Yanxia, Wang, Lu, and Wang, Yugang
- Subjects
- *
HOMEOSTASIS , *WEIGHT gain , *ADIPOSE tissues , *FAT cells , *TISSUE metabolism , *METABOLISM , *THRESHOLD energy - Abstract
The iron (Fe) metabolism plays important role in regulating systemic metabolism and obesity development. The Fe inside cells can form iron-sulfur (Fe–S) clusters, which are usually assembled into target proteins with the help of a conserved cluster assembly machinery. Family with sequence similarity 96A (FAM96A; also designated CIAO2A) is a cytosolic Fe–S assembly protein involved in the regulation of cellular Fe homeostasis. However, the biological function of FAM96A in vivo is still incompletely defined. Here, we tested the role of FAM96A in regulating organismal Fe metabolism, which is relevant to obesity and adipose tissue homeostasis. We found that in mice genetically lacking FAM96A globally, intracellular Fe homeostasis was interrupted in both white and brown adipocytes, but the systemic Fe level was normal. FAM96A deficiency led to adipocyte hypertrophy and organismal energy expenditure reduction even under nonobesogenic normal chow diet-fed conditions. Mechanistically, FAM96A deficiency promoted mechanistic target of rapamycin (mTOR) signaling in adipocytes, leading to an elevation of de novo lipogenesis and, therefore, fat mass accumulation. Furthermore, it also caused mitochondrial defects, including defects in mitochondrial number, ultrastructure, redox activity, and metabolic function in brown adipocytes, which are known to be critical for the control of energy balance. Moreover, adipocyte-selective FAM96A knockout partially phenocopied global FAM96A deficiency with adipocyte hypertrophy and organismal energy expenditure defects but the mice were resistant to high-fat diet-induced weight gain. Thus, FAM96A in adipocytes may autonomously act as a critical gatekeeper of organismal energy balance by coupling Fe metabolism to adipose tissue homeostasis. [Display omitted] • FAM96A deficiency did not affect systemic Fe metabolism but interrupted intracellular Fe homeostasis in adipocytes. • FAM96A deficiency led to adipocyte hypertrophy and energy expenditure reduction. • FAM96A deficiency promoted mTOR signaling in adipocytes. • FAM96A deficiency caused mitochondrial defects in brown adipocytes. • Adipocyte intrinsic FAM96A controlled adipocyte hypertrophy and energy expenditure. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
40. 5-Aza-2′-Deoxycytidine Regulates White Adipocyte Browning by Modulating miRNA-133a/Prdm16.
- Author
-
Liang, Jia, Jia, Ying, Yu, Huixin, Yan, Haijing, Shen, Qingyu, Xu, Yong, Li, Yana, and Yang, Meizi
- Subjects
BROWN adipose tissue ,WHITE adipose tissue ,ADIPOSE tissues ,FAT cells ,DNA demethylation ,DNA methylation ,ENERGY metabolism - Abstract
The conversion of white adipocytes into brown adipocytes improves their thermogenesis and promotes energy consumption. Epigenetic modifications affect related genes and interfere with energy metabolism, and these are the basis of new ideas for obesity treatment. Neonatal mice show high levels of DNA hypermethylation in white adipose tissue early in life and low levels in brown adipose tissue. Thus, we considered that the regulation of DNA methylation may play a role in the conversion of white adipose to brown. We observed growth indicators, lipid droplets of adipocytes, brown fat specific protein, and miRNA-133a after treatment with 5-Aza-2′-deoxycytidine. The expression of Prdm16 and Ucp-1 in adipocytes was detected after inhibiting miRNA-133a. The results showed a decrease in total lipid droplet formation and an increased expression of the brown fat specific proteins Prdm16 and Ucp-1. This study indicated that 5-Aza-2′-deoxycytidine promotes white adipocyte browning following DNA demethylation, possibly via the modulation of miR-133a and Prdm16. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
41. Peptidoglycan inhibits beigeing of adipose tissue
- Author
-
Hong Chen, Lijun Sun, Lu Feng, Michael Mulholland, Weizhen Zhang, and Yue Yin
- Subjects
Beige adipocytes ,Brown adipocytes ,Obesity ,Metabolic inflammation ,Therapeutics. Pharmacology ,RM1-950 - Published
- 2022
- Full Text
- View/download PDF
42. ChREBP plays a pivotal role in the nutrient-mediated regulation of metabolic gene expression in brown adipose tissue.
- Author
-
Baek, Seungwoo, Seo, Dong Soo, Kang, Jaehyeon, Ahmad, Yusra, Park, Sungjun, Joo, Sungmin, Kim, KyeongJin, and Jang, Younghoon
- Subjects
- *
GENETIC regulation , *TRANSCRIPTION factors , *HIGH-carbohydrate diet , *ADIPOSE tissues , *GENE expression , *BROWN adipose tissue - Abstract
Carbohydrate-responsive element-binding protein (ChREBP) is a transcription factor that regulates several metabolic genes, including the lipogenic enzymes necessary for the metabolic conversion of carbohydrates into lipids. Although the crucial role of ChREBP in the liver, the primary site of de novo lipogenesis, has been studied, its functional role in adipose tissues, particularly brown adipose tissue (BAT), remains unclear. In this study, we investigated the role of ChREBP in BAT under conditions of a high-carbohydrate diet (HCD) and ketogenic diet (KD), represented by extremely low carbohydrate intake. Using an adeno-associated virus and Cas9 knock-in mice, we rapidly generated Chrebp brown adipocyte-specific knock-out (B-KO) mice, bypassing the necessity for prolonged breeding by using the Cre-Lox system. We demonstrated that ChREBP is essential for glucose metabolism and lipogenic gene expression in BAT under HCD conditions in Chrebp B-KO mice. After nutrient intake, Chrebp B-KO attenuated the KD-induced expression of several inflammatory genes in BAT. Our results indicated that ChREBP, a nutrient-sensing regulator, is indispensable for expressing a diverse range of metabolic genes in BAT. • Rapid generation of Chrebp B-KO mice using CRISPR-Cas9 and AAV • ChREBP is essential for glucose metabolism and lipogenic gene expression in BAT. • Chrebp B-KO attenuates KD-induced inflammatory gene expression in BAT. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. The Role of Proton-Coupled Amino Acid Transporter 2 (SLC36A2) in Cold-Induced Thermogenesis of Mice
- Author
-
Hui Shu, Jie Zhang, Dawei Cheng, Xiaorui Zhao, Yue Ma, Chi Zhang, Yong Zhang, Zhihao Jia, and Zhiwei Liu
- Subjects
proton-coupled amino acid transporter ,oxygen consumption ,brown adipocytes ,thermogenesis ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Brown adipocytes mainly utilize glucose and fatty acids to produce energy, which play key roles in thermogenesis. Furthermore, brown adipocytes also utilize other substrates, such as amino acids, for energy expenditure in various conditions. Here, we report the new physiological roles of proton-coupled amino acid transporters, SLC36A2 and SLC36A3, on global energy metabolism. The relative mRNA expression levels of both Slc36a2 and Slc36a3 were all highest in brown adipose tissue. We then generated global Slc36a2 and Slc36a3 knockout mice to investigate their functions in metabolism. Neither loss of Slc36a2 nor Slc36a3 affected the body weight and body composition of the mice. Slc36a2 knockout mice exhibited increased oxygen consumption during the daytime. After cold treatment, inhibition of Slc36a2 significantly decreased the mass of brown adipose tissue compared to wildtype mice, while it lowered the expression level of Cpt1a. Moreover, the serum lipid levels and liver mass were also decreased in Slc36a2 knockout mice after cold treatment. On the contrary, Slc36a3 knockout impaired glucose tolerance and up-regulated serum LDL-cholesterol concentration. Thus, SLC36A2 and SLC36A3 play central and different roles in the energy metabolism of the mice.
- Published
- 2023
- Full Text
- View/download PDF
44. A sensitive mitochondrial thermometry 2.0 and the availability of thermogenic capacity of brown adipocyte.
- Author
-
Xiao-Yan Meng, Dian-Dian Wang, Tao-Rong Xie, Run-Zhou Yang, Chun-Feng Liu, Dan-Hua Liu, Shu-Ang Li, Yi Luan, and Jian-Sheng Kang
- Subjects
THERMOMETRY ,MITOCHONDRIA ,FAT cells ,METHYL formate ,ENZYME metabolism - Abstract
The temperature of a living cell is a crucial parameter for cellular events, such as cell division, gene expressions, enzyme activities and metabolism. We previously developed a quantifiable mitochondrial thermometry 1.0 based on rhodamine B methyl ester (RhB-ME) and rhodamine 800 (Rh800), and the theory for mitochondrial thermogenesis. Given that the synthesized RhB-ME is not readily available, thus, a convenient mitochondrial thermometry 2.0 based on tetra-methyl rhodamine methyl ester (TMRM) and Rh800 for the thermogenic study of brown adipocyte was further evolved. The fluorescence of TMRM is more sensitive (~1.4 times) to temperature than that of RhB-ME, then the TMRM-based mito-thermometry 2.0 was validated and used for the qualitatively dynamic profiles for mitochondrial thermogenic responses and mitochondrial membrane potential in living cells simultaneously. Furthermore, our results demonstrated that the heterogenous thermogenesis evoked by ß3 adrenoceptor agonist only used overall up to ~46% of the thermogenic capacity evoked by CCCP stimulation. On the other hand, the results demonstrated that the maximum thermogenesis evoked by NE and oligomycin A used up to ~79% of the thermogenic capacity, which suggested the maximum thermogenic capacity under physiological conditions by inhibiting the proton-ATPase function of the mitochondrial complex V, such as under the cold activation of sympathetic nerve and the co-release of sympathetic transmitters. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
45. Exploring the anti-obesity effects of kimchi through enhanced thermogenesis in differentiated T37i brown adipocytes.
- Author
-
Yun YR, Lee JE, Lee S, and Hong SW
- Abstract
Background: Previous research has demonstrated the anti-obesity effects of kimchi in 3T3-L1 adipocytes and mice with diet-induced obesity by assessing the expression of obesity-associated genes. Additionally, recent studies have identified mechanisms involving thermogenesis that support these effects., Objective: This study aims to further investigate the anti-obesity properties of kimchi, focusing on its impact on thermogenic activity in differentiated T37i brown adipocytes., Design: The study first evaluated the antioxidant potential of kimchi using total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) assays. Optimal differentiation conditions for T37i adipocytes were established before proceeding with evaluations of cell viability, intracellular triglyceride (TG) content, lipid accumulation, and the expression of genes and proteins related to obesity and thermogenesis., Results: Kimchi maintained over 90% cell viability in T37i adipocytes at concentrations up to 1,000 μg/mL. Efficient differentiation of T37i preadipocytes was achieved using a medium containing 10% calf serum, 2 nM 3,3',5-triiodo-L-thyronin (T3), and 100 nM insulin. Kimchi significantly reduced intracellular TG levels and lipid accumulation, compared to the control group, and enhanced the expression of genes and proteins related to thermogenesis while reducing the expression of obesity-related genes., Discussion: The findings suggest that kimchi exerts its anti-obesity effects by modulating thermogenic and obesity-related pathways in brown adipocytes, which may be partially attributed to its antioxidant properties., Conclusions: Kimchi shows promise as a preventive measure against obesity by influencing metabolic pathways associated with both obesity and thermogenesis in T37i brown adipocytes., Competing Interests: The authors declare no conflicts of interest., (© 2024 Ye-Rang Yun et al.)
- Published
- 2024
- Full Text
- View/download PDF
46. Disruption of mitochondria-associated ER membranes impairs insulin sensitivity and thermogenic function of adipocytes
- Author
-
Chih-Hao Wang, Chen-Hung Wang, Pen-Jung Hung, and Yau-Huei Wei
- Subjects
mitochondria-associated ER membranes ,insulin resistance ,type 2 diabetes ,reactive oxygen species ,white adipocytes ,brown adipocytes ,Biology (General) ,QH301-705.5 - Abstract
The prevalence and healthcare burden of obesity and its related metabolic disorders such as type 2 diabetes (T2D) are increasing rapidly. A better understanding of the pathogenesis of these diseases helps to find the therapeutic strategies. Mitochondria and endoplasmic reticulum (ER) are two important organelles involved in the maintenance of intracellular Ca2+ and ROS homeostasis. Their functional defects are thought to participate in the pathogenesis of insulin resistance or T2D. The proper structure and function of the mitochondria-associated ER membranes (MAMs) is required for efficient communication between the ER and mitochondria and defects in MAMs have been shown to play a role in metabolic syndrome and other diseases. However, the detailed mechanism to link MAMs dysfunction and pathogenesis of insulin resistance or T2D remains unclear. In the present study, we demonstrated that the proteins involved in .MAMs structure are upregulated and the formation of MAMs is increased during adipogenic differentiation of 3T3-L1 preadipocytes. Disruption of MAMs by knocking down GRP75, which is responsible for connecting ER and mitochondria, led to the impairment of differentiation and ROS accumulation in 3T3-L1 preadipocytes. Most importantly, the differentiated 3T3-L1 adipocytes with GRP75 knockdown displayed inactivation of insulin signaling pathway upon insulin stimulation. Moreover, GRP75 knockdown impaired thermogenesis and glucose utilization in brown adipocytes, the adipocytes with abundant mitochondria that regulate whole-body energy homeostasis. Taken together, our findings suggest that MAMs formation is essential for promoting mitochondrial function and maintaining a proper redox status to enable the differentiation of preadipocytes and normal functioning such as insulin signaling and thermogenesis in mature adipocytes.
- Published
- 2022
- Full Text
- View/download PDF
47. PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes
- Author
-
Jiefu Wang, Yasuhiro Onogi, Martin Krueger, Josef Oeckl, Ruth Karlina, Inderjeet Singh, Stefanie M. Hauck, Regina Feederle, Yongguo Li, and Siegfried Ussar
- Subjects
Brown adipocytes ,Lysosomal acidification ,Proton-coupled amino acid transporter ,Transporter translocation across membranes ,V-ATPase assembly ,Internal medicine ,RC31-1245 - Abstract
Objective: Brown adipocytes play a key role in maintaining body temperature as well as glucose and lipid homeostasis. However, brown adipocytes need to adapt their thermogenic activity and substrate utilization to changes in nutrient availability. Amongst the multiple factors influencing brown adipocyte activity, autophagy is an important regulatory element of thermogenic capacity and activity. Nevertheless, a specific sensing mechanism of extracellular amino acid availability linking autophagy to nutrient availability in brown adipocytes is unknown. Methods: To characterize the role of the amino acid transporter PAT2/SLC36A2 in brown adipocytes, loss or gain of function of PAT2 were studied with respect to differentiation, subcellular localization, lysosomal activity and autophagy. Activity of vATPase was evaluated by quenching of EGFP fused to LC3 or FITC-dextran loaded lysosomes in brown adipocytes upon amino acid starvation, whereas the effect of PAT2 on assembly of the vATPase was investigated by Native-PAGE. Results: We show that PAT2 translocates from the plasma membrane to the lysosome in response to amino acid withdrawal. Loss or overexpression of PAT2 impair lysosomal acidification and starvation-induced S6K re-phosphorylation, as PAT2 facilitates the assembly of the lysosomal vATPase, by recruitment of the cytoplasmic V1 subunit to the lysosome. Conclusions: PAT2 is an important sensor of extracellular amino acids and regulator of lysosomal acidification in brown adipocytes.
- Published
- 2022
- Full Text
- View/download PDF
48. Reports from World Institute of Kimchi Advance Knowledge in Obesity (Exploring the anti-obesity effects of kimchi through enhanced thermogenesis in differentiated T37i brown adipocytes).
- Published
- 2024
49. Perivascular brown adipocytes-derived kynurenic acid relaxes blood vessel via endothelium PI3K-Akt-eNOS pathway
- Author
-
Huan Wang, Jian Li, Zheng Wang, Yanfeng Tian, Chunlei Li, Feng Jin, Jia Li, and Lanfeng Wang
- Subjects
Kynurenic acid ,Kynurenine aminotransferase ,Vascular tone ,ENOS ,Brown adipocytes ,Perivascular adipose tissue ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Objective: Several metabolites from the kynurenine pathway of tryptophan metabolism play a critical role in vascular function and vascular wall remodeling. This study aimed to test whether metabolite kynurenic acid (KYNA) from the kynurenine pathway relaxes blood vessels. Approach and results: We employed histological staining, in vitro cell culture, Western blotting, real-time PCR, and nitric oxide detection to validate kynurenine aminotransferase (KAT) localization in the vasculature as well as KYNA action on endothelial cells. We also detected vascular reactivity by organ chamber and monitored blood pressure by telemetry to investigate the regulation effect of KYNA on vascular tone. The results presented that perivascular adipose tissue (PVAT) from mice thoracic aorta had robust staining of anti-KAT1 and KYNA than PVAT from the abdominal aorta and mesenteric artery, which is consistent with the expression profile of brown adipocyte marker uncoupling protein 1. KYNA, metabolized from kynurenine by KAT, relaxed pre-contracted both aortic ring and mesenteric artery. In addition, KYNA derived from KAT in PVAT participates in the cross-talk between PVAT and vessel by mediating PVAT inhibition on agonist-induced thoracic aorta contraction. Furthermore, intraperitoneal injection of KYNA in mice reduced blood pressure. The vessel relaxation effect of KYNA was through the endothelium-dependent PI3K-Akt-eNOS pathway. Finally, the high-fat diet decreased KAT1 expression in perithoracic aortic fat and led to KYNA reduction in blood. Conclusions: Our research identified KYNA generated by KAT as a novel perivascular brown adipocyte-derived vascular relaxation factor and suggests that KYNA reduction is a critical event in vascular dysfunction under obese condition.
- Published
- 2022
- Full Text
- View/download PDF
50. Fractionation of Averrhoa bilimbi hexane extract corresponding to brown adipocytes stimulation [version 3; peer review: 1 approved, 1 approved with reservations]
- Author
-
Mohamad Faiz Hamzah, Azimah Amanah, and Wai Kwan Lau
- Subjects
Brief Report ,Articles ,Averrhoa bilimbi ,obesity ,brown adipocytes ,white adipocytes ,adipomyocytes - Abstract
Averrhoa bilimbi is a fast-growing tree widely found in countries of tropical Asia. Due to easy accessibility and traditional knowledge, various parts of this plant are adopted as folk medicine and a natural health remedy. Recently, beneficial effects of bilimbi in combating obesity including its potential antihyperlipidemic and hypoglycemic activities have been discovered. This paper reports the successive extraction, partitioning and fractionation of bioactive compounds from the leaf of bilimbi that corresponds to brown adipocyte activation. In this study, the bilimbi crude ethanolic extract underwent bioassay-guided partitioning with increading polarity namely n-hexane ( n-Hex), ethyl acetate (EtOAc), n-butanol ( n-BuOH) and aqueous (H 2O). The n-hexane partition extract exhibited highest brown adipogenesis potential via adipomyocytes differentiation. Further fractionation of this active partition extract yielded 10 fractions. Gas chromatography-mass spectrometry (GC/MS was used to analyse the chemical constituents of active fractions.
- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.