714 results on '"Brucelosis"'
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2. Pancitopenia secundaria a infección por Brucella abortus: Una presentación poco frecuente.
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ARIAS-MARIÑO, DANIELA, GARCÍA-AGUDELO, LORENA, ROJAS-URREA, ALEJANDRO, and JULIÁN CUBILLOS-VEGA, HÉCTOR
- Abstract
Copyright of Acta Medica Colombiana is the property of Acta Medica Colombiana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
- Full Text
- View/download PDF
3. PREVALENCE AND RISK FACTORS ASSOCIATED WITH BOVINE BRUCELLOSIS IN DAIRY FARMS IN THE PROVINCE OF AZUAY-ECUADOR.
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Andrade Guzmán, Omar Santiago, Vintimilla Rojas, Andrea Elizabeth, López Espinoza, Mateo Damián, Guevara Riera, Guillermo Emilio, and Rivera Pirela, Sergio Emiro
- Subjects
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DAIRY farm management , *DAIRY farms , *BRUCELLOSIS , *ANIMAL herds , *ROSE bengal , *LOGISTIC regression analysis , *BOS - Abstract
The health of herds that are not within the official Brucellosis control program in the province of Azuay is unknown, and there may be areas with a higher frequency of seropositive herds. This paper aims to determine the prevalence and risk factors associated with bovine brucellosis in dairy farms. An epidemiological study was carried out in 436 farms, for which milk samples were taken from producers in collection centers, collecting trucks and herds. A georeferenced survey was used to collect information on the management of the herds. The milk was analyzed by indirect-ELISA, and thirty-seven farms were seropositive, obtaining a prevalence of 8,5%. The percentages of seropositivity were: Cuenca (14.84%), Girón (23.07%), Nabón (8.21%), Oña (11.53%), San Fernando (33.33%), Sevilla de Oro (7.14%), Sigsig (4.16%). The Rose Bengal and competitive ELISA tests were performed on bovines that contributed to the milk pool in 34 herds, establishing a 100%concordance of indirect ELISA to detect seronegative farms. In the logistic regression analysis, a significant association (P < 0;05) was determined between seropositivity and factors such as: geographic location, extension of the farm, exploitation system, presence of other domestic species, elimination of placental remains, reproduction system, having a higher probability of seropositivity in herds that presented abortions (OR = 2;71), estrus problems (OR = 2;09), birth of weak calves (OR = 3;24) and extensive management (OR = 3;67). These findings constitute serological evidence that Brucella spp. circulates in farms in the area. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
4. Seroprevalencia y factores de riesgo de brucelosis y leptospirosis en cerdos en comunidades rurales de Argentina.
- Author
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Analía Silva, Julia, Alejandro Scialfa, Exequiel, Elena Gutiérrez, Silvina, Tisnés, Adela, Gastón Rodríguez, Marcelo, Marcela Estein, Silvia, and Alejandra Rivero, Mariana
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ENDEMIC diseases , *ZOONOSES , *AGRICULTURE , *LEPTOSPIROSIS , *AGGLUTINATION tests , *SWINE breeding , *HORSE breeding - Abstract
Objectives. To determine the seroprevalence of brucellosis and leptospirosis in pigs reared on smallscale farms in rural areas of the district of Tandil, Buenos Aires Province, Argentina; to analyse the associated risk factors; to describe the prevalent Leptospira spp. serogroups; and to determine the spatial distribution of both diseases. Materials and methods. A total of 340 serum samples were obtained. Buffer Plate Agglutination Test, Rose Bengal Test, and Fluorescence Polarisation Assay were used to detect antibodies against Brucella spp. Microscopic Agglutination Test was applied for serological diagnosis of leptospirosis. Statistical methods were used to evaluate the factors associated with the infections. Results. Seroprevalence of 0% and 22.6% was observed for brucellosis and leptospirosis, respectively. The most prevalent Leptospira serogroups identified were Canicola, Ballum, Icterohaemorrhagiae and Pomona. The main risk factors associated with leptospirosis infection were farms with a higher number of pigs and the presence of horses and wild boars near or within the farms. The use of milling by-products of cereal grains as animal feed and the presence of weaning cages were also associated. A relevant spatial cluster of seropositivity to Leptospira spp. was identified in a low-altitude area. Conclusions. Our results suggest that brucellosis is probably controlled, but Leptospira spp. are present in the farming systems under study. Knowledge of brucellosis and leptospirosis seroprevalence, spatial distribution and associated risk factors can be useful for the prevention and control of endemic zoonotic diseases in the region. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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- View/download PDF
5. Evaluation of tumor necrosis Factor Alpha, Interferon Gamma, Procalcitonin and Neopterin levels in Brucella seropositive cattle.
- Author
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Tuzcu, Nevin, Tuzcu, Mehmet, and Akcakavak, Gokhan
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TUMOR necrosis factors ,CALCITONIN - Abstract
Copyright of Revista Cientifica de la Facultade de Veterinaria is the property of Universidad del Zulia, Facultad de Ciencias Veterinarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
6. Prevalencia y factores de riesgo asociados a brucelosis bovina en ganaderías lecheras de la provincia del Azuay-Ecuador
- Author
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Omar Santiago Andrade Guzmán, Andrea Elizabeth Vintimilla Rojas, Mateo Damián López Espinoza, Guillermo Emilio Guevara Viera, and Sergio Emiro Rivera Pirela
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Prevalencia ,brucelosis ,ensayo inmunoadsorvente ligado a enzimas ,factores de riesgo ,Agriculture ,Agriculture (General) ,S1-972 ,Science (General) ,Q1-390 - Abstract
Se desconoce el estatus sanitario de ganaderías que no están dentro del programa oficial de control de Brucelosis en la provincia del Azuay, pudiendo existir zonas con mayor frecuencia de rebaños seropositivos. Este trabajo pretende determinar la prevalencia y factores de riesgo asociados a brucelosis bovina en predios lecheros. Se llevó a cabo un estudio epidemiológico en 436 fincas, para lo cual se tomaron muestras de leche de productores en centros de acopio, camiones recolectores y hatos. Se usó una encuesta georeferenciada a fin de recopilar información del manejo de las ganaderías. La leche se analizó mediante ELISA-indirecto, 37 fincas resultaron seropositivas, obteniendo una prevalencia de 8,5%. Los porcentajes de seropositividad fueron: Cuenca (14,84%), Girón (23,07%), Nabón (8,21%), Oña (11,53%), San Fernando (33,33%), Sevilla de Oro (7,14%), Sigsig (4,16%). Se realizaron las pruebas Rosa de Bengala y ELISA-competitivo a bovinos que aportaron al pool de leche en 34 ganaderías, estableciéndose una concordancia del 100% de ELISA-indirecto para detectar fincas seronegativas. En el análisis de regresión logística se determinó una asociación significativa (P < 0;05) entre la seropositividad y factores como: ubicación geográfica, extensión de la finca, sistema de explotación, presencia de otras especies domésticas, eliminación de restos placentarios, sistema de reproducción, teniendo una mayor probabilidad de seropositividad las ganaderías que presentaron abortos (OR = 2;71), problemas de celo (OR = 2;09), nacimiento de terneros débiles (OR=3,24) y manejo extensivo (OR = 3;67). Estos hallazgos constituyen evidencia serológica que Brucella spp. circula en ganaderías de la zona.
- Published
- 2023
7. UTILIZAÇÃO DE SISTEMA DE DIGITALIZAÇÃO E PROCESSAMENTO DE INFORMAÇÕES PARA O DIAGNÓSTICO DA BRUCELOSE EQUINA.
- Author
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de Nardi Junior, Geraldo, de Oliveira, Paulo André, Aparecido Martins, Edson, Franco, José Rafael, and Martins, Luiza Christophano
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INFORMATION storage & retrieval systems ,IDENTIFICATION of animals ,HORSE health ,BRUCELLOSIS ,ANIMAL experimentation - Abstract
Copyright of Revista Foco (Interdisciplinary Studies Journal) is the property of Revista Foco and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
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8. Evaluation of polymorphisms at the 3´ UTR SLC11A1 gene microsatellites and their associations with the outcome of Brucella abortus infection in Bos taurus cattle
- Author
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F.C. Hasenauer, M.E. Caffaro, M.A. Poli, and Carlos A. Rossetti
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bovinos ,resistencia ,brucelosis ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
Los polimorfismos presentes en los microsatélites de la región 3’UTR del gen SLC11A1 de los rumiantes fue asociada a la resistencia natural a la infección por Brucella spp. y Mycobacterium spp., aunque su relevancia en la prevención de la brucelosis bovina es controversial. El objetivo de este estudio fue reevaluar el rol de esos polimorfismos frente a una infección por B. abortus en bovinos de razas europeas. Inicialmente se utilizó la presencia o ausencia de anticuerpos específicos anti B. abortus en bovinos de carne (n=74) o leche (n=69) con alto riesgo de infección natural para identificar animales susceptibles (casos, infectados) o resistentes (controles, no infectados) a la infección. Posteriormente, la resistencia innata a la infección por B. abortus fue evaluada en macrófagos derivados de monocitos sanguíneos (MDMs) desafiados con la bacteria. Finalmente, se realizó un análisis bioinformático de la porción 3’UTR del gen SLC11A1 para evaluar el impacto funcional en la regulación del gen. Se genotiparon por electroforesis capilar – PCR multiplex para ambos microsatélites, 54 animales serológicamente positivos y 89 negativos a brucelosis. Nuestros resultados mostraron que los genotipos 159 y 175 para los Ms1 y Ms2 respectivamente, previamente definidos como “resistentes”, fueron los más frecuentes entre la población estudiada. Independientemente de esto, no se detectó asociación entre estos u otros polimorfismos con la ausencia o presencia de respuesta inmune humoral a Brucella. Tampoco se observó asociación entre los genotipos resistentes y el fenotipo de crecimiento de B. abortus en MDMs. El análisis in silico de la secuencia 3’ UTR predijo dos sitios de unión canónicos para elementos reguladores transcripcionales pertenecientes a las familias TEF-1 y SMAD, además de indicar que la estructura secundaria de esa porción génica permanecía inalterable independientemente de la extensión de los microsatélites. En conjunto, estos resultados indican una falta de asociación entre los polimorfismos en la porción 3’UTR del gen SLC11A1 y la resistencia natural a la brucelosis en los bovinos de origen europeos.
- Published
- 2022
9. Desarrollo de una vacuna veterinaria en contexto semiperiférico: DeltaPgM contra la brucelosis bovina.
- Author
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Zubeldía Brenner, Lautaro and Surtayeva, Sofya
- Subjects
TECHNOLOGICAL innovations ,VACCINE development ,BRUCELLOSIS ,EXPORT marketing ,BOS - Abstract
Copyright of Revista Iberoamericana de Ciencia, Tecnologia y Sociedad is the property of Centro de Estudios sobre Ciencia, Desarrollo y Educacion Superior and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
10. Prevalencia y factores de riesgo asociados a brucelosis bovina en ganaderías lecheras de la provincia del Azuay-Ecuador
- Author
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Andrade, Omar S., Vintimilla, Andrea E., López Espinoza, Mateo Damián, Guevara Viera, Guillermo, Rivera Pirela, Sergio, Andrade, Omar S., Vintimilla, Andrea E., López Espinoza, Mateo Damián, Guevara Viera, Guillermo, and Rivera Pirela, Sergio
- Abstract
The health of herds that are not within the official Brucellosis control program in the province of Azuay is unknown, and there may be areas with a higher frequency of seropositive herds. This paper aims to determine the prevalence and risk factors associated with bovine brucellosis in dairy farms. An epidemiological study was carried out in 436 farms, for which milk samples were taken from producers in collection centers, collecting trucks and herds. A georeferenced survey was used to collect information on the management of the herds. The milk was analyzed by indirect-ELISA, and thirty-seven farms were seropositive, obtaining a prevalence of 8,5%. The percentages of seropositivity were: Cuenca (14.84%), Girón (23.07%), Nabón (8.21%), Oña (11.53%), San Fernando (33.33%), Sevilla de Oro (7.14%), Sigsig (4.16%). The Rose Bengal and competitive ELISA tests were performed on bovines that contributed to the milk pool in 34 herds, establishing a 100%concordance of indirect ELISA to detect seronegative farms. In the logistic regression analysis, a significant association (P < 0;05) was determined between seropositivity and factors such as: geographic location, extension of the farm, exploitation system, presence of other domestic species, elimination of placental remains, reproduction system, having a higher probability of seropositivity in herds that presented abortions (OR = 2;71), estrus problems (OR = 2;09), birth of weak calves (OR = 3;24) and extensive management (OR = 3;67). These findings constitute serological evidence that Brucella spp. circulates in farms in the area., Se desconoce el estatus sanitario de ganaderías que no están dentro del programa oficial de control de Brucelosis en la provincia del Azuay, pudiendo existir zonas con mayor frecuencia de rebaños seropositivos. Este trabajo pretende determinar la prevalencia y factores de riesgo asociados a brucelosis bovina en predios lecheros. Se llevó a cabo un estudio epidemiológico en 436 fincas, para lo cual se tomaron muestras de leche de productores en centros de acopio, camiones recolectores y hatos. Se usó una encuesta georeferenciada a fin de recopilar información del manejo de las ganaderías. La leche se analizó mediante ELISA-indirecto, 37 fincas resultaron seropositivas, obteniendo una prevalencia de 8,5%. Los porcentajes de seropositividad fueron: Cuenca (14,84%), Girón (23,07%), Nabón (8,21%), Oña (11,53%), San Fernando (33,33%), Sevilla de Oro (7,14%), Sigsig (4,16%). Se realizaron las pruebas Rosa de Bengala y ELISA-competitivo a bovinos que aportaron al pool de leche en 34 ganaderías, estableciéndose una concordancia del 100% de ELISA-indirecto para detectar fincas seronegativas. En el análisis de regresión logística se determinó una asociación significativa (P < 0;05) entre la seropositividad y factores como: ubicación geográfica, extensión de la finca, sistema de explotación, presencia de otras especies domésticas, eliminación de restos placentarios, sistema de reproducción, teniendo una mayor probabilidad de seropositividad las ganaderías que presentaron abortos (OR = 2;71), problemas de celo (OR = 2;09), nacimiento de terneros débiles (OR=3,24) y manejo extensivo (OR = 3;67). Estos hallazgos constituyen evidencia serológica que Brucella spp. circula en ganaderías de la zona.
- Published
- 2024
11. [Risk factors associated with the presence of brucellosis in mixed establishments (caprine and bovine) in the province of Formosa, Argentina].
- Author
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Carolina Elías I, Viola MN, Russo AM, and Signorini Porchietto M
- Abstract
Brucellosis is a zoonotic disease with a worldwide distribution that causes significant productive losses and negative impacts on public health. The aim of this work was to identify the risk factors associated with the presence of brucellosis in mixed farms (cattle/goats) in the central-western region of the province of Formosa. Sixty seven mixed farms with 7,855 animals (2,943 cattle and 4,912 goats) were randomly sampled during 2022/2023. The samples were serologically analyzed (BPA + FPA) to determine which animals were positive. Likewise, in each farm, a confidential survey was carried out to evaluate potential associated variables. The statistical analysis was performed using Generalized Linear Mixed Models (GLMM) with binomial distribution, logarithmic link function, and farm as a random variable. The estimated prevalence of brucellosis in mixed farms was 9%, while the joint prevalence per animal (cattle and goats) was 2.75%. The prevalence of brucellosis in cattle and goats was explained by the presence of animals with reproductive signs (odds ratio, OR=40), the occurrence of abortions in the last term of gestation and the birth of weak offspring (OR=5.3), incorrect treatment of abortions (OR=8), the introduction of animals from other farms (OR=5.9), and not having a negative brucellosis certificate (OR=9.6). The management practices conducted in each farm such as preventive measures, sanitation practices for the pens, and prevention measures for the workers, are important for the control of brucellosis., (Copyright © 2024 The Author(s). Publicado por Elsevier España, S.L.U. All rights reserved.)
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- 2024
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- View/download PDF
12. Las políticas públicas en salud para el cuidado especial a la Brucelosis.
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Vidal del Río, Mildre Mercedes, González Salas, Raúl, and Monsalve Guamán, Aldemar Alejandro
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HEALTH policy ,HUMAN-to-human transmission ,BIBLIOGRAPHIC databases ,BRUCELLOSIS ,BREAST milk ,ZOONOSES ,BRUCELLA - Abstract
Copyright of Dilemas Contemporáneos: Educación, Política y Valores is the property of Dilemas Contemporaneos: Educacion, Politica y Valores and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
13. Seroprevalencia de Brucella canis en perros de un refugio para animales de compañía en Bogotá, Colombia
- Author
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Astrid-Jullieth Laverde, Daniela Restrepo-Botero, Diego Hernández-Pulido, José Luis Rodríguez-Bautista, and Isabel-Sofía Sandoval
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brucelosis ,perros ,zoonosis ,cromatografía de afinidad ,salud pública ,Medicine ,Arctic medicine. Tropical medicine ,RC955-962 - Abstract
Introducción. El riesgo de infección con Brucella canis en humanos y perros aumenta con la exposición constante a perros portadores asintomáticos. En Colombia hay evidencia de infección con B. canis en personas que conviven con perros. Una preocupación adicional en Bogotá es la falta de información actualizada sobre la prevalencia de la infección en perros destinados a programas de adopción. Objetivo. Establecer la seroprevalencia de la infección por B. canis en perros de un refugio para animales de compañía destinados a la adopción en Bogotá. Materiales y métodos. Se hizo un estudio descriptivo de corte transversal en un refugio ara animales de Bogotá. Se detectaron anticuerpos contra B. canis en el suero de 51 perros (28 hembras y 23 machos) mediante una prueba inmunocromatográfica de flujo lateral. Asimismo, los individuos positivos se analizaron con PCR para la detección del ADN de Brucella spp. Resultado. La seroprevalencia de B. canis fue del 1,96 % (1/51). El perro seropositivo correspondió a una hembra asintomática de tres años de edad en la cual no se detectó ADN bacteriano en sangre mediante la PCR. Conclusiones. La seroprevalencia representada por un solo perro con IgG anti-B. canis puede considerarse un riesgo potencial para las poblaciones de perros y humanos, ya que podría tratarse de un animal con infección persistente capaz de diseminar la bacteria.
- Published
- 2021
- Full Text
- View/download PDF
14. Seroprevalencia de anticuerpos contra la Brucella sp. en ganaderos del distrito San José de Lourdes, Cajamarca
- Author
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Escolana Julcahuanca, Edit Pérez, Cinthya Santa Cruz-López, and Marcela Saldaña
- Subjects
brucelosis ,estudios seroepidemiológicos ,pruebas serológicas ,comunidad rural ,Medicine (General) ,R5-920 - Abstract
Objetivo. Determinar la seroprevalencia de anticuerpos contra la Brucella sp. e identificar los factores predisponentes y la sintomatología asociada en los ganaderos del distrito San José de Lourdes (San Ignacio) en el departamento de Cajamarca, durante los meses de setiembre a diciembre del 2019. Métodos. El estudio fue de tipo observacional, transversal, prospectivo con un diseño exploratorio-descriptivo. Se realizó un muestreo probabilístico, donde se analizaron 147 sueros sanguíneos pertenecientes a ganaderos de ambos géneros. La seroprevalencia de anticuerpos se determinó mediante las pruebas Rosa de Bengala y antígeno de Huddleson y se aplicó una encuesta estructurada para identificar los factores predisponentes y la sintomatología asociada a la enfermedad. Resultados. Se encontró que, 17 % de ganaderos seropositivos a Brucella sp. mantuvo contacto directo prolongado con el ganado bovino (p < 0,05). Además, el 23,8 % de ganaderos presentó anticuerpos contra la Brucella sp, siendo principalmente mujeres (15,6 %). Los ganaderos con anticuerpos contra la Brucella sp. consumían queso (22,4 %), leche (19,7 %) y sangre de animales sacrificados (17 %) (p > 0,05). Los síntomas más recurrentes fueron el dolor de cabeza (19 %), fatiga (14,3 %), dolor articular (13,6 %), fiebre e insomnio (10,2 %). Conclusiones. La seroprevalencia de anticuerpos contra la Brucella sp. es alta y está asociada al contacto directo prolongado con el ganado bovino y a síntomas como el dolor de cabeza y la fiebre, presentes en la población evaluada. Los resultados obtenidos permiten evidenciar la preocupante situación que viven los ganaderos de San José de Lourdes, además de la escasa cultura preventiva existente.
- Published
- 2021
- Full Text
- View/download PDF
15. Evaluación de indicadores productivos en rebaños caprinos vacunados con cepas RB51–SOD, RB51 (Brucella abortus) y Rev-1 (Brucella melitensis)
- Author
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Baldomero Molina-Sánchez, David Itzcóatl Martínez-Herrera, Violeta Trinidad Pardío-Sedas, Ricardo Flores-Castro, José A. Villagómez-Cortés, and José F. Morales-Álvarez
- Subjects
vacunación ,abortos ,brucelosis ,cabras ,rb51- sod ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Se determinaron tasas de pariciones, abortos y nacimiento de crías débiles en rebaños vacunados con la cepa RB51–SOD (B. abortus) para evaluar la mejora productiva y compararla con las vacunas Rev–1 (B. melitensis) y RB51 (B. abortus). Se vacunaron tres subgrupos de 36 cabras cada uno con cepas Rev–1 (1–2x109 UFC), RB51 (3x108-3x109 UFC) y RB51–SOD (3x108-3x109 UFC), cada cepa tuvo un subgrupo control. Se establecieron registros individuales para calcular las tasas posvacunación en dos épocas de pariciones. En la primera, la tasa de partos para Rev-1 fue 66.6 % (IC95%: 48.9–80.9), RB51 50.0 % (IC95% :33.2–66.7) y RB51-SOD 69.4 % (IC95% :51.7–83.0). Los abortos se presentaron en los tres subgrupos vacunados, Rev-1 y RB51-SOD 5.5 % (IC95% :0.9–20.0) y RB51 2.7 % (IC95%: 0.1–16.2). Los nacimientos de crías débiles se presentaron solo en animales vacunados con Rev-1 5.5 % (IC95%: 0.9–20.0). Durante la segunda época, la tasa de partos en hembras vacunadas con Rev-1 fue de 91.6 % (IC95% :76.4–97.8), RB51 94.4 % (IC95% :79.9–99.0) y RB51-SOD 94.4 % (IC95% :79.9–99.0). Tuvieron abortos animales vacunados con cepas Rev-1 y RB51, 5.5 % (IC95%: 0.9–20.0) y 2.7 % (IC95%: 0.1–16.2), respectivamente; en subgrupos vacunados no hubo nacimiento de crías débiles. Los subgrupos controles tuvieron un comportamiento similar a los vacunados. Los animales vacunados con la cepa RB51–SOD no mostraron diferencia significativa con los que recibieron las cepas Rev-1 y RB51, ni con los subgrupos control (P>0.01); por lo anterior, la vacuna RB51-SOD puede generar protección contra la brucelosis y beneficios en la producción de rebaños caprinos.
- Published
- 2021
- Full Text
- View/download PDF
16. Tres zoonosis erróneamente subestimadas pueden incrementar su impacto global bajo el manto de la covid-19.
- Author
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Barreto Argilagos, Guillermo, Rodríguez Torrens, Herlinda, and Barreto Rodríguez, Herlinda
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ZOONOSES , *COVID-19 pandemic , *MEDICAL care , *TUBERCULOSIS , *TWENTY-first century , *PREVENTIVE medicine , *TWENTIETH century - Abstract
Since ancient times, both humans and animals have suffered from three diseases: tuberculosis, leptospirosis and brucellosis. During the 80's of the 20th century, it was assumed that they were totally under control, but there was an illadvised decision that made possible their underestimation and persistence up to the present. The purpose of this review was to warn about erroneous criteria that sustain the underestimation of three lethal zoonoses whose effects will be exacerbated in the midst of COVID-19. Although there are multiple factors involved, it is emphasized that: a) there has never been total control of these diseases; b) they are not an exclusive problem of the less socially and economically favored geographic areas; c) the deficit of resources for diagnosis and health services in the latter limits the real statistics of their worldwide impact; and d) most research is not focused from the One Health approach, being a bias that minimizes or obviates the effects of the zoonotic component and the environment. The above limitations, despite the fact that tuberculosis has been recognized as the most lethal pandemic to humanity, have delayed the decision for its global eradication until 2018. The unquestionable attention demanded by COVID-19, as a pandemic since 2020, will further affect the care of these diseases and their control, thus increasing their unnoticed lethal impact. Addressing these mistakes would be a first step towards the adoption of global measures aimed at a more effective control of these three ancestral diseases in force in the 21st century. [ABSTRACT FROM AUTHOR]
- Published
- 2022
17. Aislamiento de Brucella abortus en equinos con bursitis supurativa supraespinosa, dedicados al arreo de bovinos.
- Author
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Juan, López-Toledo, Fernando, Hidalgo-y-Terán-Serralde, Erika, Palomares-Resendiz, José, Morales-Álvarez, Guadalupe, Martínez-Serrano, and Efrén, Diaz-Aparicio
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ANIMAL herds , *BRUCELLOSIS , *BRUCELLA abortus , *CATTLE , *EQUIDAE , *SYMPTOMS , *DONKEYS , *CATTLE herding - Abstract
In Mexico there are no reports of Brucella abortus isolation in equines. The objective of this study was to isolate B. abortus in equines with clinical signs of chronic suppurative bursitis on the withers and nape of the neck, dedicated to herding cattle in a dual-purpose herd located in Tabasco, Mexico. In the 48 working equids of this farm, horses, donkeys and mules, a serological card test was performed, resulting 25% positive, of them three horses and one mule, which presented abscesses and fistulas of the withers and nape, samples of the purulent material were collected by aspiration, from the four exudate samples B. abortus was isolated and identified by conventional methods and PCR. We report the first isolation in Mexico of B. abortus from working horses and mules from a herd of cattle with brucellosis. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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18. Brucella suis y virus de Aujeszky en producciones familiares porcinas de la Provincia La Pampa, Argentina.
- Author
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Murcia, V. N., Beneitez, A., Giménez, H., Lorda, H., and Fort, M.
- Subjects
FAMILY farms ,SWINE farms ,HEALTH literacy ,BRUCELLA ,BLOOD sampling - Abstract
Copyright of Revista Veterinaria is the property of Universidad Nacional del Nordeste and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
- Full Text
- View/download PDF
19. Effects of vaccination with Brucella melitensis, strains Rev 1 ΔeryCD and Rev 1, on the reproductive system of young male goats.
- Author
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López Vásquez IA, Palomares Resendiz EG, Gutiérrez Hernández JL, Garrido Fariña GI, Tórtora Pérez JL, de Jesús Olmos Espejel J, Hernández Castro R, Sangari F, Suárez Güemes F, and Díaz Aparicio E
- Subjects
- Animals, Male, Vaccination, Genitalia, Male microbiology, Bacterial Vaccines, Goats, Brucella melitensis immunology, Brucellosis prevention & control, Brucellosis veterinary, Brucellosis microbiology, Brucella Vaccine immunology, Brucella Vaccine administration & dosage
- Abstract
The present study evaluates the effects of vaccination with Brucella melitensis strains Rev 1 ΔeryCD and Rev 1 on the reproductive system of male goats. Three groups, each of them consisting of 15 six-month-old brucellosis-free male goats, were studied. The first group was vaccinated with the Rev 1 ΔeryCD strain, the second group received Rev 1 and the third group was inoculated with sterile physiological saline solution. The dose of both strains was of 1×10
9 CFU/ml. Over the course of the five months of this study, three males from each group were euthanized every month. Their reproductive tracts, spleens, and lymph nodes were collected to analyze serology, bacteriology PCR, histology, and immunohistochemistry. Results show that vaccination with B. melitensis strains Rev 1 ΔeryCD and Rev 1 does not harm the reproductive system of male goats. Strain B. melitensis Rev 1 ΔeryCD displayed a lower capacity to colonize the reproductive tract than strain Rev 1, which was attributed to its limited catabolic action toward erythritol., (Copyright © 2024 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)- Published
- 2024
- Full Text
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20. Interactive web-based tool for evaluating the spread of bovine tuberculosis and brucellosis in Colombia.
- Author
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Arenas, Nelson E., Ávila, Edier F., Correa, Edwin D., Rueda, Wendy N., López, Gina M., and Soto, Carlos Y.
- Subjects
- *
TUBERCULOSIS in cattle , *BRUCELLOSIS , *TUBERCULOSIS , *DISEASE outbreaks , *LIVESTOCK losses , *COMMUNICABLE diseases , *DISEASE mapping - Abstract
Background: Bovine tuberculosis (BTB) and brucellosis are associated with devastating losses in the livestock sector in Colombia and even in developed countries. Real-time disease surveillance is a key strategy to control and eradicate infectious disease outbreaks. Objective: To design an epidemiological tool for monitoring BTB and brucellosis in Colombia. Methods: An interactive platform for disease mapping of BTB and brucellosis during an observation period between years 2004 and 2019 was designed. Results: Our analysis showed that the provinces of Cundinamarca and Valle del Cauca are regions affected by BTB and brucellosis epidemics, respectively (p<0.001). Furthermore, increased case detection of BTB was reported in 2012 and brucellosis in 2019 (p<0.001). Conclusions: This epidemiological platform allows tracking BTB and tuberculosis hotspots, identifying trends over time, and provides useful information to animal health authorities for designing new strategies in control programs. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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21. Brucelosis como causa de fiebre persistente en un trabajador de la salud de Cali, Colombia. Reporte de caso.
- Author
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Araque-Villaquirán, F., Peña-Guevara, A. A., Carvajal-Mazuera, A., and Loaiza Giraldo, J.
- Subjects
ENZYME-linked immunosorbent assay ,BRUCELLOSIS ,AGGLUTINATION tests ,OCCUPATIONAL exposure ,MEDICAL personnel ,BRUCELLA ,Q fever - Abstract
Copyright of Revista Cuarzo (REC) is the property of Fundacion Universitaria Juan N. Corpas and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
22. Lateral flow biosensor combined with loop-mediated isothermal amplification for simple, rapid, sensitive, and reliable detection of Brucella spp
- Author
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Li S, Liu Y, Wang Y, Chen H, and Liu C
- Subjects
Brucella spp. ,Brucelosis ,Loop-mediated isothermal amplification ,Lateral flow biosensor ,Limit of detection ,Infectious and parasitic diseases ,RC109-216 - Abstract
Shijun Li,1 Ying Liu,1 Yue Wang,1 Hong Chen,2 Chunting Liu,1 Yi Wang3,41Laboratory of Bacterial Infectious Disease of Experimental Center, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, People’s Republic of China; 2Laboratory of Guiyang Center for Animal Disease Control and Prevention, Guiyang 550081, People’s Republic of China; 3Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 10045, People’s Republic of China; 4Ministry of Education, National Key Discipline of Pediatrics (Capital Medial University), Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 10045, People’s Republic of ChinaAbstract: Brucella species is responsible for brucellosis in human and animals, which is still of public health, veterinarian, and economic concern in many regions of the world. Here, a novel molecular diagnosis assay, termed loop-mediated isothermal amplification coupled with nanoparticles-based lateral flow biosensor (LAMP-LFB), was developed and validated for simply, rapidly, and reliably detecting all Brucella spp. strains. A set of six primers was designed based on the Brucella-specific gene Bscp31. The Brucella-LAMP results were visually reported by biosensor within 2 mins. A variety of bacterial strains representing several Brucella species, as well as several Gram-negative and Gram-positive bacterial species were used to determine the analytical sensitivity and specificity of the assay. Optimal LAMP conditions were 63°C for 40 mins, and the assay’s sensitivity was found to be 100 fg of genomic DNA in the pure cultures. No cross-reactions to non-Brucella strains were obtained; thus, analytical specificity of LAMP-LFB assay is of 100%. Using the protocol, 20 mins for rapid DNA preparation followed by isothermal amplification (40 mins) combined with biosensor detection (2 mins) resulted in a total assay time of approximately 65 mins. In the case of 117 whole blood samples, 13 (11.11%) samples were Brucella-positive by LAMP-LFB, and the diagnostic accuracy was 100% when compared to the culture-biotechnical method. In conclusion, Brucella-LAMP-LFB technique developed in this study is a sensitive and specific method to rapidly identify all Brucella spp. strains, and can be applied as a potential diagnostic tool for brucellosis in basic, clinical, and field laboratories.Keywords: Brucella spp., brucellosis, loop-mediated isothermal amplification, lateral flow biosensor, limit of detection
- Published
- 2019
23. Estudio de 14 casos de brucelosis en trabajadores de un frigorífico como enfermedad profesional
- Author
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Adriana Pisani, Mariela Vacarezza, and Fernando Tomasina
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brucelosis ,condiciones de trabajo ,Medicine ,Medicine (General) ,R5-920 - Abstract
El presente estudio se realizó en 14 trabajadores de una misma industria frigorífica que contrajeron brucelosis, diagnosticados y asistidos en el marco del seguro de accidentes y enfermedades profesionales de nuestro país. Los trabajadores concurrieron a la policlínica de medicina laboral del Departamento de Salud Ocupacional, Hospital de Clínicas, durante los años 2009 y 2010 para asesoramiento en salud laboral. El objetivo del presente trabajo fue describir y caracterizar las condiciones laborales y las manifestaciones clínicas de 14 trabajadores de una misma empresa con diagnóstico de brucelosis que concurrieron a la policlínica del departamento en el período comprendido de la revisión. Para ello se realizó el estudio descriptivo en base a fuente preexistente aportada por los trabajadores diagnosticados con brucelosis. Las entrevistas se llevaron a cabo en la consulta ambulatoria de Departamento de Salud Ocupacional, en el período comprendido entre enero de 2009 a julio de 2010. Los datos obtenidos a partir de las historias clínicas cedidas por los involucrados y de la historia laboral se ingresaron a una base estadística (EPI info 2000). De los 14 individuos estudiados, dos correspondieron a mujeres y doce a hombres, que presentaron como síntomas más frecuentes: astenia, mialgias, fiebre, artralgias, sudoración y cefaleas. Ocho de los trabajadores pertenecían al sector faena, tres a mondonguería, vísceras y corrales, dos a limpieza y uno a mantenimiento. La época del año donde se objetivó un mayor número de casos fue enero, febrero y marzo de 2009, lo que coincide con un aumento en la faena de animales. El tiempo de asistencia fue prolongado y coincide con otros trabajos consultados. Conclusiones: las características del proceso laboral y la presencia del agente biológico en el ambiente son determinantes en la aparición de la enfermedad en los trabajadores expuestos. Los pilares fundamentales en la prevención de esta enfermedad se basan en la capacitación de trabajadores y empleadores. Son imprescindibles mejoras en el ambiente general de trabajo y en las condiciones en las que se realiza la tarea como nexo vincular entre el proceso y el probable daño a la salud.
- Published
- 2019
24. Seroprevalencia de enfermedades abortivas que comprometen la eficiencia reproductiva de los bovinos en dos zonas lecheras de Chiapas
- Author
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José Bernardo Sánchez Muñoz, Miriam Liseth Jiménez Jiménez, Jose Luis Gutierrez Hernandez, José Luis Cruz López, and José Nahet Toral
- Subjects
Rumiantes ,Brucelosis ,Leptospirosis ,Neosporosis ,Social sciences (General) ,H1-99 - Abstract
El objetivo del presente estudio fue determinar la seroprevalencia de anticuerpos contra Brucella abortus, Leptospira spp y Neospora caninum en bovinos en dos zonas lecheras del estado de Chiapas. Se colectó el suero sanguíneo de 76 y 103 bovinos hembra de los municipios de Tecpatán y Juárez respectivamente. La detección de anticuerpos contra Brucella abortus se realizó mediante la prueba de Tarjeta y su confirmación con Rivanol, micro aglutinación en placa contra siete serovariedades de Leptospira, e Inmunoensayo enzimático contra Neospora caninum. Los resultados encontrados muestran una seroprevalencia de anticuerpos para Brucelosis del 0 y 6.8%; 29 y 63% para leptospirosis para el municipio de Tecpatán y Juárez respectivamente, siendo los serovares de mayor frecuencia de seropositividad Icteroahemorrhagiae y Tarassovi, en ambos municipios, mientras que la seropositividad contra Neospora caninum fue de un 46 y 21% respectivamente. Se concluye que la presencia de anticuerpos contra Brucella, Leptospira y Neospora caninum pueden estar relacionados con la eficiencia reproductiva de los bovinos en los municipios de Tecpatán y Juárez, en el estado de Chiapas.
- Published
- 2021
- Full Text
- View/download PDF
25. Detección de anticuerpos de Brucella spp. en mataderos del estado Bolívar y Soledad, municipio Independencia, estado Anzoátegui, Venezuela, 2006
- Author
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Julman Rosiris Cermeño and Ricardo De Grazia
- Subjects
Bovinos ,brucelosis ,seroprevalencia ,Cattle ,SF191-275 ,Veterinary medicine ,SF600-1100 - Abstract
El objetivo de este estudio fue determinar la seroprevalencia humana y bovina de anticuerpos contra Brucella spp. en mataderos del estado Bolívar y Soledad, municipio Independencia, estado Anzoátegui, Venezuela. Para ello, se realizó un estudio prospectivo y transversal. Previo permiso por escrito de autoridades competentes y consentimiento informado de los trabajadores, se seleccionaron 7 mataderos. Se registraron datos de interés epidemiológico y clínico. Se extrajo sangre por venopunción de personas y bovinos. La demostración de anticuerpos contra Brucella spp. se realizó mediante la prueba DRG® Brucella IgM ELISA en humanos y ELISA competitiva, Brucella-Ab C-ELISA de SVANOVIR®, en animales. Se evaluaron 462 sueros: 159 de trabajadores y 303 de bovinos. La prevalencia de infección en humanos fue 5,6 % y de bovinos 11,2 %; el mayor porcentaje se obtuvo en el grupo de los trabajadores con edades entre 21 y 30 años (a) (P = 0,15) y de sexo masculino (P = 0,34). Siendo más frecuente en el personal que tenía menos de 10 a con riesgo ocupacional (66,6 %); correspondiendo a la ocupación de matarife y despostador el mayor número de casos. El 55,6 % de los trabajadores consumían lácteos sin pasteurizar. Las manifestaciones clínicas fueron inespecíficas: artralgias, cefalea y sudoración nocturna (P > 0,05). Se demuestra que la mayoría de trabajadores de los mataderos evaluados del estado Bolívar y Soledad, municipio Independencia del estado Anzoátegui, están expuestos a múltiples factores de riesgo para contraer brucelosis, sin embargo, la seroprevalencia de anticuerpos de Brucella spp. en ellos fue baja (5,6 %) aunque elevada entre los bovinos estudiados (11,2 %).
- Published
- 2021
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26. Seroprevalencia de Brucella canis en perros de un refugio para animales de compañía en Bogotá, Colombia.
- Author
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Laverde, Astrid-Jullieth, Restrepo-Botero, Daniela, Hernández-Pulido, Diego, Rodríguez-Bautista, José Luis, and Sandoval, Isabel-Sofía
- Abstract
Introduction: The risk of Brucella canis infection in humans and dogs has increased due to the permanent exposure to asymptomatic carrier dogs. In Colombia, there is evidence of B. canis infection in humans living with dogs. In the case of Bogotá, an additional concern is the lack of updated information related to the prevalence of the infection in dogs. Objective: To determine the seroprevalence of infection by B. canis in dogs intended for adoption programs in Bogotá. Materials and methods: By means of a descriptive cross-sectional study carried out in a dog shelter in Bogotá, anti-B. canis IgG antibodies were detected in the serum from 51 dogs (28 females and 23 males) using a lateral-flow immunochromatographic test. Additionally, seropositive animals were analyzed with PCR to detect Brucella spp DNA. Results: Brucella canis seroprevalence was 1.96% (1/51). The seropositive dog was an asymptomatic three-year-old she-dog in which no bacteria DNA was detected in the blood through PCR. Conclusions: The seroprevalence determined in this study represented by a single dog with anti-B. canis IgG can be considered a potential risk both for canine and human populations since this single dog could have a persistent infection capable of spreading the bacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
27. Detección de anticuerpos de Brucella spp. en mataderos del estado Bolívar y Soledad, municipio Independencia, estado Anzoátegui, Venezuela, 2006.
- Author
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Rosiris Cermeño, Julman and De Grazia, Ricardo
- Abstract
Copyright of Revista Cientifica de la Facultade de Veterinaria is the property of Universidad del Zulia, Facultad de Ciencias Veterinarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
28. Evaluación de indicadores productivos en rebaños caprinos vacunados con cepas RB51-SOD, RB51 (Brucella abortus) y Rev-1 (Brucella melitensis).
- Author
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Molina-Sánchez, Baldomero, Izcoatl Martínez-Herrera, David, Trinidad Pardío-Sedas, Violeta, Flores-Castro, Ricardo, Villagómez-Cortés, José A., and Morales-Álvarez, José F.
- Subjects
- *
VACCINATION , *BRUCELLOSIS , *MISCARRIAGE , *GOATS , *ANIMAL herds - Abstract
Kidding rates, miscarriages and births of weak offspring were determined in herds vaccinated with the RB51-SOD (B. abortus) strain in order to evaluate the productive improvement and compare it with Rev-1 (B. melitensis) and RB51 (B. abortus) vaccines. Three subgroups of 36 goats each were vaccinated with Rev-1 (1-2x109 CFU), RB51 (3x108-3x109 CFU) and RB51-SOD (3x108-3x109 CFU) strains, with each strain having a control subgroup. Individual records were established for calculating post-vaccination rates in two kidding seasons. In the first, the kidding rate for Rev-1 was 66.6 % (95%CI: 48.9-80.9), RB51 50.0 % (95%CI:33.2-66.7), and RB51-SOD 69.4 % (95%CI:51.7-83.0). Miscarriages occurred in all three vaccinated subgroups, Rev-1 and RB51-SOD 5.5 % (95%CI: 0.9-20.0) and RB51 2.7 % (95%CI: 0.1-16.2). Weak offspring births occurred only in animals vaccinated with Rev-1 5.5 % (95%CI: 0.9-20.0). During the second epoch, the kidding rate in Rev-1 vaccinated females was 91.6 % (95% CI:76.4-97.8), RB51 94.4 % (95% CI:79.9-99.0), and RB51-SOD 94.4 % (95% CI:79.9-99.0). Animals vaccinated with Rev-1 and RB51 strains had 5.5 % (95%CI: 0.9-20.0) and 2.7 % (95%CI: 0.1-16.2) miscarriages, respectively; in vaccinated subgroups there were no births of weak offspring. The control subgroups behaved similarly to the vaccinated subgroups. Animals vaccinated with the RB51-SOD strain showed no significant difference from those that received the Rev-1 and RB51 strains, nor from the control subgroups (P>0.01); therefore, the RB51-SOD vaccine can generate protection against brucellosis and benefits in the production of goat herds. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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29. Brucelosis, aspectos que limitan su justa valoración.
- Author
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Barreto Argilagos, Guillermo, Rodríguez Torrens, Herlinda, and Barreto Rodríguez, Herlinda
- Subjects
- *
BRUCELLOSIS , *ZOONOSES , *RESEARCH bias , *BIOFILMS - Abstract
Brucellosis, one of the ancient zoonoses, is still among the most widespread in the world today. This persistence is due in part to the misunderstanding behavior at a certain point in time, when the disease was considered to have been eradicated. Its current underestimation may be due to research biases that distort its real impact since almost all research over 133 years has focused on the B. melitensis planktonic phenotype and not in its behavior as biofilm; and because its prevention and control measures respond to these results. The purpose of this review was to provide elements, which were not previously published, for a closer understanding of the disease known as brucellosis. [ABSTRACT FROM AUTHOR]
- Published
- 2021
30. Seroprevalence of Brucella canis and Leptospira spp. in canines in the city of Medellín, Colombia.
- Author
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López-Diez, Laura, Ortiz-Román, Luisa, Sanchez-Nodarse, Raúl, Sanabria-Gonzalez, William, Henao-Correa, Enrique, and Olivera-Angel, Martha
- Subjects
- *
LEPTOSPIRA , *CANIS , *BRUCELLA , *ZOONOSES , *LEPTOSPIROSIS , *SEROPREVALENCE - Abstract
Introduction: The agents that cause diseases of zoonotic importance in canines, such as Canine brucellosis and Leptospirosis, have gained importance in human clinical practice. Objective: To discover the prevalence and behavior of both diseases in the canine population in the city of Medellín to develop measures of prevention and control in this area. Methods: A total of 1,300 canines were sampled to test for Brucella canis and Leptospira spp. using the PARP-2ME and MAT techniques, respectively, to establish the statistical significance of the different variables analyzed (P = 0.05; OR = 1; 95% CI). Results: Seroprevalence was determined to be 7.32% for B. canis and 9.08%, for Leptospira spp. with a 0.77% co-infection rate of both diseases. The most prevalent serovars for Leptospira spp. were Canicola (3.38%), Icterohaemorrhagiae (2.62%), and Pomona (0.92%). A statistical association was reported for B. canis with the commune variable (San Javier P = 0.002; OR = 2.724 / Guayabal P = 0.000; OR = 3.862 / Belén P = 0.002; OR = 2.953), and for Leptospira spp. with the commune variable (Buenos Aires P = 0.011; OR = 2.220) and age (37-48 months P = 0.005; OR = 4.272). Conclusions: This study shows that both agents are in circulation among the canine population in the city and in all the communes analyzed, representing a possible risk of infection to owners and other animals entering into contact with them. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
31. Seroprevalencia de brucelosis y leptospirosis en pobladores urbanos con crianza traspatio en el distrito de José Leonardo Ortiz de Chiclayo.
- Author
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Yamunaqué-Castro, Luis A., Aguilar-Gamboa, Franklin R., Quenema-Diaz, Eyner A., Becerra-Gutiérrez, Lizzie K., and Silva-Díaz, Heber
- Subjects
- *
BRUCELLOSIS , *ECOLOGY , *ENZYME-linked immunosorbent assay , *HUMAN reproductive technology , *IMMUNOGLOBULINS , *LEPTOSPIROSIS , *RESEARCH , *RESTROOMS , *RODENTS , *SURVEYS , *URBAN health , *CITY dwellers , *PSYCHOSOCIAL factors , *CROSS-sectional method , *SEROPREVALENCE , *DESCRIPTIVE statistics , *DISEASE risk factors - Abstract
Objective: To describe the seroprevalence of and risk factors for brucellosis and leptospirosis in urban citizens with backyard breeding in the district of José Leonardo Ortiz, Chiclayo. Methods: Cross-sectional study including 90 citizens during the month of October and December of 2016. IgM and IgG specific antibodies for brucellosis and leptospirosis were determined using the indirect ELISA method. Factors associated with these diseases were gathered using a structural survey. Results: The seroprevalence of brucellosis and leptospirosis was 2.2% and 28.9%, respectively. The most common environmental features of the population were that 73.3% use latrines 83.3% had contact with stagnant water and 62.2% had contact with rodents. No association between the variables looked for and these two diseases was found. Conclusions: We found a high seroprevalence of leptospirosis but a low prevalence of brucellosis in this setting. We suggest performing longitudinal studies that may identify risk factors to prevent these diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
32. Frecuencia de enfermedades de impacto reproductivo en bovinos de doble propósito ubicados en Oaxaca, México.
- Author
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José, Gutiérrez-Hernández, Gabriela, Palomares-Resendiz, Erik, Hernández-Badillo, José, Leyva-Corona, Efrén, Díaz-Aparicio, and Enrique, Herrera-López
- Subjects
- *
BOVINE viral diarrhea , *AGGLUTINATION tests , *LEPTOSPIROSIS , *CATTLE herding , *BRUCELLOSIS - Abstract
Brucellosis, leptospirosis, bovine viral diarrhea (BVD) and bovine infectious rhinotracheitis (IBR) are abortive diseases that compromise productive efficiency in cattle. In different municipalities of Oaxaca, Mexico, reproductive problems suggestive of these diseases have been observed. This study determined the frequency of DVB, IBR, brucellosis and leptospirosis in dual-purpose cattle herds in different regions of Oaxaca Mexico. A total of 2,691 blood samples were collected from 127 bovine herds to diagnose brucellosis serologically using the rose bengal and Rivanol tests; microscopic agglutination test (MAT) with a battery of six Leptospira serovars; ELISA by blocking and indirect for BVD and IBR respectively. The apparent overall frequency of herd was brucellosis: 2.3%, leptospirosis: 86.6%, IBR: 65.4% and BVD: 56.7%. The real frequency for brucellosis, leptospirosis, IBR and BVD was -9.1% (CI95 = -10.1, -7.9%), 64.3% (CI95 = 62.5, 66.1%), 40.1% (IC95 = 38.2, 41.9%) and 33.2 % (IC95 = 31.4, 35.0%) respectively. In the Costa region, the highest percentage of animals with antibodies against Leptospira, IBR and BVD was observed. The present study showed serologically the presence of antibodies against brucellosis, IBR, DVB and six serovars of Leptospira in bovine herds double purpose of the state of Oaxaca, Mexico. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
33. Evaluación de un antígeno de Brucella abortus para aglutinación en placa como prueba tamiz en el diagnóstico de la brucelosis bovina
- Author
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David Rajme-Manzur, Marian Hernández-Reyes, and Meilyn Cruz-Soca
- Subjects
Brucella abortus ,aglutinación en placa ,brucelosis ,Medicine - Abstract
Este trabajo tuvo como objetivo obtener y validar un antígeno buferado de Brucella abortus para la prueba de aglutinación en placa como prueba diagnóstica de base de la brucelosis bovina. Se formularon tres lotes de antígeno a partir de la multiplicación de la cepa 99 de Brucella abortus. Se realizaron los controles de calidad correspondientes (determinación de pH, volumen celular, esterilidad, capacidad buferante) y las pruebas serológicas para la evaluación del desempeño. Se emplearon 1070 muestras de suero bovino (350 positivas y 720 negativas) previamente controladas con las pruebas de diagnóstico establecidas. Se determinó la sensibilidad y especificidad diagnóstica y relativa, los valores predictivos positivos y negativos, la eficacia y la concordancia. En los tres lotes todas las características evaluadas resultaron estar dentro de los parámetros establecidos para este tipo de producto. La especificidad y sensibilidad diagnósticas fueron de 99,5% y 100% respectivamente. El valor predictivo positivo fue de 99,1%, el valor predictivo negativo fue de 100% y la eficacia de un 99,7%. El antígeno mostró una sensibilidad y especificidad relativas de un 100% y la concordancia resultó ser clasificada como muy buena. La evaluación del desempeño arrojó resultados satisfactorios, demostrando que el método de producción empleado es factible para la obtención de un producto con adecuada eficacia.
- Published
- 2017
34. Posibles casos de tuberculosis y brucelosis en poblados argáricos de Galera (Granada)
- Author
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Ángel Rubio, Sylvia A. Jiménez-Brobeil, Lydia P. Sánchez-Barba, Zita Laffranchi, and Fernando Molina
- Subjects
península ibérica ,edad del bronce ,zoonosis ,tuberculosis ,brucelosis ,Archaeology ,CC1-960 - Abstract
Una de las características principales de las poblaciones argáricas de la Edad del Bronce de Granada es la existencia de una economía agropecuaria con la presencia de animales dentro de los núcleos poblacionales, lo que supone un riesgo de contagio. En este estudio se presentan unos casos atribuibles a dos enfermedades infecciosas de esta índole hallados en los poblados de Castellón Alto y Fuente Amarga, ambos en Galera (provincia de Granada). En estos yacimientos se han detectado cuatro sujetos que muestran formaciones de hueso nuevo en el tórax (escápulas y costillas) que pueden ser indicativas de tuberculosis y además, en Fuente Amarga, un individuo presenta una lesión característica en la columna vertebral atribuible a brucelosis (epifisitis vertebral). Estos procesos no son de extrañar en poblaciones que tuvieron un contacto estrecho con animales.
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- 2017
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35. Características epidemiológicas y la tendencia de incidencia de la brucelosis en el condado de Kohgiluyeh, Irán, periodo 2011-2015.
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Naimi, Ebrahim, Nasirian, Maryam, Piraee, Elahe, and Faghihi, Sayed Hassan
- Abstract
Copyright of Dilemas Contemporáneos: Educación, Política y Valores is the property of Dilemas Contemporaneos: Educacion, Politica y Valores and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
36. ORQUIEPIDIDIMITIS AGUDA POR BRUCELLA. PRESENTACIÓN DE UN CASO.
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Matheu, A. Ramos
- Abstract
Copyright of Revista Chilena de Urologia is the property of Sociedad Chilena de Urologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
37. Brucelosis en ovinos y caprinos en explotaciones de manejo intensivas y extensivas del estado Lara, Venezuela
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Francisco Vargas, Dr., Caribay Chirinos, Msc., Raúl Rojas, Msc, Yvenioski Gamarra, Msc, and Ortelio Mosquera, Msc
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Brucelosis ,caprinos ,ovinos ,ELISAc ,Rosa de Bengala ,Social Sciences - Abstract
El objetivo del estudio que dio origen a este artículo fue determinar el porcentaje de seropositividad a brucelosis, en ovinos y caprinos de explotaciones intensivas y extensivas del Estado Lara. Se evaluó un total de 400 caprinos mayores de 6 meses de edad, pertenecientes a 21 explotaciones y todos los ovinos (169) de la misma edad, que convivían con los caprinos en dichas explotaciones. Del total de la muestra de caprinos analizadas, 297 pertenecían a explotaciones intensivas y 103 a extensivas. En relación a los ovinos analizados, 118 pertenecían a explotaciones intensivas y 51 a extensivas. Se obtuvieron muestras de suero de estos animales, las cuales fueron procesadas en laboratorio mediante la prueba de Rosa de Bengala (Card Test) convencional y ELISA Competitivo para brucelosis (ELISAc). Como resultado de la prueba de ELISAc, 40 caprinos (13,4%) fueron positivos en explotaciones intensivas y 3 caprinos (2,9%) fueron positivos en explotaciones extensivas. En relación a los ovinos, 23 animales (19%) fueron positivos en explotaciones intensivas y 8 animales (15,7%), fueron positivos en explotaciones extensivas. Ninguno de los sueros analizados resultó positivo a la prueba de Rosa de Bengala convencional, esta prueba no fue útil para el diagnóstico de brucelosis en estas especies a diferencia del ELISAc. Los caprinos positivos mostraron signos de infertilidad con menor porcentaje de preñez y menor número de partos al año, y no abortos. Se concluye: la infección por Brucella está presente en los ovinos y caprinos de esta localidad.
- Published
- 2016
38. Detección molecular de Brucella sp, Leptospira spp y Toxoplasma gondii en felinos domésticos y silvestres y el impacto zoonótico en el personal encargado de su manejo en Pereira, Colombia
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Caballero Méndez, Lyda C., Franco Montoya, Luz Natalia, Mazo, Margarita María, Sepúlveda-Arias, Juan Carlos, Cardona Tabares, Andrés, and Caro, Juan José
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PCR ,brucellosis ,felinos ,brucelosis ,leptospirosis ,feline ,toxoplasmosis - Abstract
The study aimed to determine the presence of the infectious agents that cause toxoplasmosis, brucellosis and leptospirosis using the conventional PCR molecular technique in domestic and wild cats, as well as in the personnel related to the care and management of these animals in the city of Pereira, Risaralda. (Colombia). Blood samples were taken from domestic cats (n=99) and wild cats in captivity (n=4) and from the personnel responsible for their care and management (veterinarians, zootechnicians, and operators; n=65). A prevalence of 37% for toxoplasmosis and 1% for leptospirosis in domestic felines was estimated. The presence of these pathogens was not found in the personnel., El estudio buscó determinar la presencia de los agentes infecciosos causantes de la toxoplasmosis, brucelosis y leptospirosis empleando la técnica molecular PCR convencional en felinos domésticos y silvestres, así como en el personal relacionado con el cuidado y manejo de estos animales en la ciudad de Pereira, Risaralda (Colombia). Se tomaron muestras de sangre de felinos domésticos (n=99) y silvestres en cautiverio (n=4) y del personal responsable de su cuidado y manejo (médicos veterinarios, zootecnistas, y operarios; n=65). Se estimó una prevalencia de 37% para toxoplasmosis y de 1% para leptospirosis en felinos domésticos. No se encontró la presencia de los patógenos en el personal.
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- 2023
39. Seroprevalence of antibodies to Brucella sp in goats Malargüe department , Mendoza
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Daniel Alberto Baruta, Pablo Mauricio Lowinger, and Ezequiel Mariani
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brucelosis ,caprinos ,Veterinary medicine ,SF600-1100 - Abstract
Se realizó un relevamiento en 1.200 caprinos pertenecientes a 16 establecimientos del Departamento Malargüe en la Provincia de Mendoza mediante la prueba de Antígeno Bufferado en Placa (BPA). A los positivos se los expuso a seroaglutinación en tubo (SAT) y 2 Mercaptoetanol para confirmar la presencia de anticuerpos resistentes. Se obtuvo 3,83% de animales serológicamente positivo
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- 2017
40. PREDOMINO DE LA Brucella abortus EN CANES EN UN MUNICIPIO DE LA REGIÓN TROPICAL AMERICANA
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Francisco Feliciano da Silva Junior, José Andreey Almeida Teles, Rael Lucas Fonseca de Almeida, Gil Dutra Furtado, Aleudson dos Santos Silva, and Breno Stefano Carvalho Tabosa
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Brucelosis ,Canes ,Test Serológicos ,Zoonosis. ,Environmental sciences ,GE1-350 - Abstract
La brucelosis canina es una enfermedad infecciosa de potencial zoonótico que afecta humanos, mamíferos domésticos y silvestres. La presencia de esta enfermedad en las hembras caninas, puede presentar muerte embrionaria precoz, abortamiento en el último tercio de gestación, altos índices de fetos expulsados muertos en el momento del parto; mientras que en los machos pueden presentar infertilidad, como consecuencia de alteraciones en los gametos masculinos, epididimitis, orquitis y dermatitis escrotal. También existen relatos de señales clínicas de inflamación intraocular, alteraciones en las vértebras, inflamación en las meninges, infección en los riñones y dermatitis pio granulomatosa. Generalmente es causada por la Brucella canis, y eventualmente, Brucella abortus, puede estar involucrada. Los métodos serológicos han sido los más utilizados para el diagnóstico de la brucelosis. El objetivo de este trabajo fue evaluar la seropredominancia de la Brucella abortus en canes del municipio de Sao Miguel dos Campos, Alagoas. La investigación utilizó 203 muestras de sangre, para obtención de suero. Estas fueron sometidas a las pruebas serológicas del Antígeno Acidificado Tamponado (AAT) en temperatura ambiente. De las 203 muestras, 31 fueron positivas al teste del AAT. La brucelosis, causada por B. abortus, es una enfermedad que está presente en canes del municipio de Sao Miguel dos Campos, Alagoas, siendo necesario que el poder público municipal tome providencias cuanto a la salud pública, teniendo en vista que se trata de una potencial zoonosis.
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- 2018
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41. Presence of IgG antibodies against reproductive infections in breeding bulls of Magdalena Medio, Colombia
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Rodolfo Camacho M, Lenny Y Carvajal, Yeny Z Castellanos-Dominguez, Wilson F Díaz, and María C Vásquez
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brucelosis ,diarrea viral bovina ,ensayo de inmunoabsorción enzimática ,leptospirosis ,Neospora caninum ,rinotraqueitis infecciosa bovina ,Animal culture ,SF1-1100 - Abstract
Background: cattle farming is an important economic activity in several regions of Colombia. However, farmers are unaware of their cattle health status, and particularly of bacterial, parasitic, and viral diseases, which affect reproduction and profitability. Objective: to assess the presence of IgG antibodies against infectious bovine rhinotracheitis (IBR), bovine viral diarrhea (BVD), bovine leukosis, leptospirosis, brucellosis, and Neospora caninum in breeding bulls in the Magdalena Medio of Colombia, according to age, breed, and origin. Methods: a descriptive, cross-sectional study was conducted with 174 male breeding cattle from several subspecies. Animals were above 18 months of age and belonged to dual-purpose farms in the Magdalena Medio region. Presence of IgG anti-IBR, BVD, brucellosis, neosporosis, and leukosis antibodies were determined with an enzyme-linked immunosorbent assay (ELISA). A sample of 156 males was assessed for leptospirosis using a microscopic agglutination test (MAT) in a dark field. Results: IgG anti-IBR antibodies were found in 92.5% of the animals, anti-BVD in 62.6%, anti-Neospora caninum in 79.3%, anti-Brucella abortus in 4.02%, anti-Leptospira pomona in 8.33%, and anti-L. hardjo in 15.38%, with >1:100 titres. Besides, 64.94% prevalence was observed for bovine leukosis. Conclusions: a high prevalence of IgG anti-IBR, IgG anti-BVD, IgG anti- Neospora caninum, IgG anti-leukosis, and IgG anti-lepstospirosis antibodies were observed in Magdalena Medio bulls. With respect to B. abortus, confirmatory and competitive ELISA test should be performed and sanitary standards need to be established. Detection of IgG antibodies among bulls reveals poor sanitary conditions, absent of protective-vaccination antibodies and an urgent need for health control measurements.
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- 2015
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42. Brucelosis, una zoonosis presente en la población: estudio de series de tiempo en México
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Meztli Méndez-Lozano and Luisa María Sánchez-Zamorano
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brucelosis ,zoonosis ,rumiantes ,México ,Public aspects of medicine ,RA1-1270 - Abstract
Objetivo. Determinar el comportamiento de la incidencia de brucelosis humana en México durante el periodo 2000-2011 y su relación con la brucelosis en rumiantes domésticos. Material y métodos. En estudio ecológico de series de tiempo se analizaron, mediante regresión Poisson multinivel múltiple, la incidencia de brucelosis humanas durante 2000- 2011 y la incidencia en rumiantes, y su relación con índice de desarrollo humano. Resultados. La alta incidencia de brucelosis bovina aumenta 15% la incidencia de brucelosis humana. La alta incidencia en caprinos aumenta 33% la incidencia de brucelosis humana. La presencia de casos nuevos de brucelosis ovina aumenta 13% la incidencia de brucelosis humana. Conclusiones. La brucelosis es un problema de salud pública; la presencia de la brucelosis en los rumiantes domésticos y el nivel de control de la enfermedad en ellos afecta la presencia de la enfermedad en humanos.
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- 2015
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43. Prevalence of Brucella spp in humans
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Catharina de Paula Oliveira Cavalcanti Soares, José Andreey Almeida Teles, Aldenir Feitosa dos Santos, Stemberg Oliveira Firmino Silva, Maria Vilma Rocha Andrade Cruz, and Francisco Feliciano da Silva-Júnior
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Brucelosis ,Prevalencia ,Humano ,Zoonosis ,Nursing ,RT1-120 - Abstract
Objective: to determine the seroprevalence of Brucella spp in humans.Method: this is an observational study, developed with 455 individuals between 18 and 64 years old, who use the Estratégia de Saúde da Família (Brazil's family health strategy). The serum samples of volunteers underwent buffered acid antigen tests, such as screening, agar gel immunodiffusion and slow seroagglutination test in tubes and 2-Mercaptoethanol.Results: among the samples, 1.98% has responded to buffered-acid antigen, 2.85% to agar gel immunodiffusion test and 1.54% to the slow seroagglutination tests on tubes/2-Mercaptoethanol. The prevalence of Brucella spp was 4.4%, represented by the last two tests.Conclusion: the results of this research suggest that the studied population is exposed to Brucella spp infection.
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- 2015
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44. Risk factors associated with the presentation of brucellosis in the spine
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Alberto Aceves Pérez
- Subjects
Brucelosis ,Columna vertebral ,Terapéutica ,Pronóstico ,Factores de riesgo ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
OBJECTIVE: The aim of this study is to describe the risk factors associated with the presentation of brucellosis on the spine, and determine the strength of association between these factors. METHODS: The medical records of patients with brucellosis on the spine were analyzed and a spreadsheet was created to compile the following data: age, sex, place of origin and residence, risk factors (exposure at work, consumption of unpasteurized products and comorbidities), clinical presentation (lumbar pain, anorexia, headache, myalgia, fatigue, paresthesia, dysesthesia, muscle weakness, weight loss, fever), affected spine level, presence of abscesses, disease duration (acute, subacute, chronic), laboratory studies (erythrocyte sedimentation rate, C-reactive protein, serology, blood culture, Rose Bengal test, histopathological reports), imaging studies (x-rays, MRI, bone gammagraphy), established treatment (medical and/or surgical), therapeutic failure and sequelae. RESULTS: A total of 17 patients, 10 women (58.8%) and seven men (41.2%), were reviewed from January 2007 to January 2011. The group had a mean age of 57.8 years with a standard deviation of +13.91 and age range between 16-74 years. CONCLUSIONS: There is no significant difference between the groups compared with respect to age and sex, however, improvement of the neurological deficit was observed in eight patients who underwent surgical procedure.
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- 2015
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45. Evaluation of polymorphisms at the 3´ UTR SLC11A1 gene microsatellites and their associations with the outcome of Brucella abortus infection in Bos taurus cattle
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Hasenauer, Flavia Carolina, Caffaro, María Eugenia, Poli, Mario Andres, and Rossetti, Carlos Alberto
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Microsatélites ,Ganado Bovino ,Brucella abortus ,Cattle ,Brucelosis ,Enfermedades de los Animales ,Polymorphism ,Microsatellites ,Bos taurus ,Brucellosis ,Polimorfismo ,Animal Diseases - Abstract
In ruminants, polymorphisms in microsatellites at 3´untranslated region (3´ UTR) of the SLC11A1 gene were associated with natural resistance to Brucella spp. and Mycobacterium spp. infection, but its relevance to prevent brucellosis is controversial in cattle. The aim of this study was to re-evaluate the role of these polymorphisms in the outcome of Brucella abortus infection in European bovine breeds. Initially, the presence or absence of specific antibodies against Brucella abortus in beef (n=74) or dairy (n=69) Bos taurus cattle at high risk of natural Brucella infection was used to identify susceptible (cases, infected) or resistant (control, non-infected) animals. Then, innate resistance to Brucella infection was evaluated in B. taurus peripheral blood monocyte-derived macrophages (MDMs) challenged with the pathogen. Finally, a bioinformatics analysis of the 3′ UTR of the SLC11A1 gene was performed to evaluate its putative functional impact on gene regulation. Fifty four (54) brucellosis positive and 89 brucellosis negative animals were genotyped for both microsatellites by multiplex PCR-capillary electrophoresis. Our results showed that the homozygous genotypes 159 and 175 for Ms1 and Ms2 respectively, previously defined as “resistant” genotypes, were the most frequent among the animal population. Independently, no association was detected between these or other polymorphisms and the absence or presence of humoral immune response against brucellosis. Moreover, no association was observed between the resistant genotype with the restricted B. abortus-intracellular growth phenotype in MDMs. In silico analysis of 3′ UTR sequence predicted two canonical binding sites for transcriptional regulatory elements belonging to TEF-1 and SMAD families, but most importantly, the secondary structure of the 3’UTR remains unchanged regardless of the length of the microsatellites. Taken together, these results show no evidence of an association between the 3’UTR SLC11A1 polymorphisms and natural resistance against brucellosis in cattle. Los polimorfismos presentes en los microsatélites de la región 3’UTR del gen SLC11A1 de los rumiantes fue asociada a la resistencia natural a la infección por Brucella spp. y Mycobacterium spp., aunque su relevancia en la prevención de la brucelosis bovina es controversial. El objetivo de este estudio fue reevaluar el rol de esos polimorfismos frente a una infección por B. abortus en bovinos de razas europeas. Inicialmente se utilizó la presencia o ausencia de anticuerpos específicos anti B. abortus en bovinos de carne (n=74) o leche (n=69) con alto riesgo de infección natural para identificar animales susceptibles (casos, infectados) o resistentes (controles, no infectados) a la infección. Posteriormente, la resistencia innata a la infección por B. abortus fue evaluada en macrófagos derivados de monocitos sanguíneos (MDMs) desafiados con la bacteria. Finalmente, se realizó un análisis bioinformático de la porción 3’UTR del gen SLC11A1 para evaluar el impacto funcional en la regulación del gen. Se genotiparon por electroforesis capilar– PCR multiplex para ambos microsatélites, 54 animales serológicamente positivos y 89 negativos a brucelosis. Nuestros resultados mostraron que los genotipos 159 y 175 para los Ms1 y Ms2 respectivamente, previamente definidos como “resistentes”, fueron los más frecuentes entre la población estudiada. Independientemente de esto, no se detectó asociación entre estos u otros polimorfismos con la ausencia o presencia de respuesta inmune humoral a Brucella. Tampoco se observó asociación entre los genotipos resistentes y el fenotipo de crecimiento de B. abortus en MDMs. El análisis in silico de la secuencia 3’ UTR predijo dos sitios de unión canónicos para elementos reguladores transcripcionales pertenecientes a las familias TEF-1 y SMAD, además de indicar que la estructura secundaria de esa porción génica permanecía inalterable independientemente de la extensión de los microsatélites. En conjunto, estos resultados indican una falta de asociación entre los polimorfismos en la porción 3’UTR del gen SLC11A1 y la resistencia natural a la brucelosis en los bovinos de origen europeos. Instituto de Patobiología Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Hasenauer, Flavia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hasenauer, Flavia Carolina. Université Paris Cité, CNRS, INSERM; Francia Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
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- 2022
46. IDENTIFICACIÓN DE Brucella abortus A PARTIR DE QUESO FRESCO ARTESANAL DE VACA EN VERACRUZ, MÉXICO.
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Hernández-Carbajal, Gabriela R., Martínez-Herrera, David I., Pardío-Sedas, Violeta T., Quintana-Castro, Rodolfo, Morales-Álvarez, J. Francisco, López-Hernández, Karla M., Olliart-Ros, Rosa M., Villagómez-Cortés, José A., and Huerta-Peña, Javier C.
- Abstract
Brucellosis is a zoonosis whose main source of infection is contaminated milk and unpasteurized products. The difficulty of isolating the agent limits the definitive diagnosis, so the joint use of methods such as the polymerase chain reaction (PCR) and the inoculation of susceptible animals may represent an alternative to isolate and identify Brucella spp. The objective was to identify the presence of Brucella abortus from samples of artisanal fresh cow milk cheese collected in five collection centers in the central area of the Mexican state of Veracruz using a murine infection model and PCR. The design of the study was cross-sectional epidemiological and 100 samples of cheese were collected during May and June of 2017 that were analyzed by conventional microbiology. In one sample, the presence of a S19 vaccine strain of B. abortus was identified by multiplex PCR. To try their isolation, from said sample of cheese, ten mice were inoculated intraperitoneally according to the provisions of NOM-062-ZOO-1999. The female mice were sacrificed by cervical dislocation, samples of liver, kidney, spleen and bone marrow were extracted and plated with Agar-Farell plates, which were maintained in aerobiosis for 10 d. None of the female mice were seroconverted nor colonies of Brucella spp. developed from extracted organs, but PCR diagnosis was useful to identify Brucella spp. in fresh cheeses. [ABSTRACT FROM AUTHOR]
- Published
- 2018
47. Brucelosis: prevalencia y factores de riesgo asociados en bovinos, bubalinos, caprinos y ovinos de Formosa, Argentina.
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Martinez, D. E., Cipolini, M. F., Storani, C. A., Russo, A. M., and Martinez, E. I.
- Abstract
Copyright of Revista Veterinaria is the property of Universidad Nacional del Nordeste and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2018
- Full Text
- View/download PDF
48. Desarrollo de una vacuna veterinaria en contexto semiperiférico: DeltaPgM contra la brucelosis bovina
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Zubeldía, Lautaro, Surtayeva, Sofya, Zubeldía, Lautaro, and Surtayeva, Sofya
- Abstract
The formulation of technology policies is one of the main challenges faced by semi-peripheral countries that are struggling to drive technological change. In this context, vaccines make an attractive market in Latin America, presenting a window of opportunity to generate local developments. As a example, we examine the eventful trajectory of the development of a vaccine for bovine brucellosis in Argentina, showing the institutional limitations and regulatory barriers faced by a semi-peripheral economy in its attempt to develop and position technological and industrial tools in the regional and global market., A formulação de políticas de tecnologia é um dos principais desafios enfrentados pelos países semiperiféricos para impulsionar a mudança tecnológica. Nesse contexto, as vacinas constituem um mercado atraente na América Latina, apresentando uma janela de oportunidade para gerar desenvolvimentos locais. Como exemplo específico, examinamos o caso da movimentada trajetória de desenvolvimento de uma vacina para a brucelose bovina na Argentina, mostrando as limitações institucionais e barreiras regulatórias enfrentadas por uma economia semiperiférica em sua tentativa de desenvolvimento e posição no mercado regional e global de ferramentas tecnológicas e industriais., La formulación de políticas tecnológicas es uno de los principales desafíos que enfrentan los países semiperiféricos para impulsar el cambio tecnológico. En este contexto, las vacunas conforman un mercado atractivo en América Latina, presentando una ventana de oportunidad para generar desarrollos locales. Como ejemplo específico, examinamos el caso de la accidentada trayectoria del desarrollo de una vacuna para la brucelosis bovina en Argentina, mostrando las limitaciones institucionales y las barreras regulatorias que enfrenta una economía semiperiférica en su intento de desarrollar y posicionar, en el mercado regional y global, herramientas tecnológicas e industriales.
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- 2022
49. La tuberculosis y la brucelosis: una aproximación arqueológica a los cuidados humanos prehistóricos
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León Cristóbal, Alejandro and León Cristóbal, Alejandro
- Abstract
Testu honek ikuspegi sozial eta humanistkoa eman nahi dio historiaurreko gaixotasun infekziosoen gaiari. Nahiz eta literatura zientfkoak ez duen bereziki ikertu giza zainketen gaia hainbat arrazoirengatk, bistan da iraganeko patologia infekziosoei dagokienez kontrakoa gertatu dela. Hala ere, mota horretako gaixotasunak adierazten dituzten hezur-aztarnarik ia ez dagoenez, hurrengo lerroetan, historiaurreko giza-taldeek birusen, bakterioen eta parasitoen infekzioerasoen aurrean beren hurkoei eman zizkieten zainketei eta gizarte-portaerei buruzko hausnarketa egiten da. Hain zuzen, gizarte-jokaera mota horiek, portaera sinbolikoekin batera, historiaurreko arkeologian ezezagun handienetakoak dira. Iraganeko populazioetan ondo ezagutzen diren bi patologia infekzioso (tuberkulosia eta bruzelosia) abiapuntu hartuta, bereziki jokabide sozialak eta giza zainketak azalduko dira. Gainera, gaixotasun horien hedapenak berrikuntza neolitkoak Europako lurraldeetara iristearekin zerikusi handia duela aztertuko da, eta baita gaixotasun hauek denboran zehar erakutsi duten iraunkortasuna ziurrenik gizakiongan duen prebalentziagatk dela, This text aims to provide a social and humanistc point of view of infectous diseases in Prehistory. Although the scientfc literature has not partcularly investgated the subject of human caring for various reasons, it is clear that, respectng to infectous diseases in the past, it has been the opposite case. However, given the partcular absence of skeletal remains afected by this type of disease, it is proposed, in the following lines, to provide a refecton on caring and social behaviour in the prehistoric groups from the infectous atack of viruses, bacteria and parasites. Precisely, this type of social behaviour, together with symbolic behaviour, is one of the greatest unknowns in prehistoric archaeology. Based on two well-recognised infectous pathologies in the past, such as tuberculosis and brucellosis, it will be made special reference to the social behaviour and human caring analysis. It can be observed that the spread of these diseases has much in common with the arrival of Neolithic innovatons in Europe, when several of these diseases had a startng point of persistence over the tme thanks to their human’s prevalence, El presente texto busca aportar una visión social y humanístca a la cuestón de las enfermedades infecciosas durante la Prehistoria. Pese a que la literatura cientfca no ha indagado especialmente en la temátca de los cuidados humanos por causas diversas, resulta evidente que en lo que respecta a las patologías infecciosas del pasado sí ha sucedido todo lo contrario. No obstante, dada la especial ausencia de restos óseos afectados por este tpo de enfermedades, se propone, en las próximas líneas, aportar una refexión al respecto de los cuidados y comportamientos sociales que los grupos prehistóricos brindaron a sus semejantes ante el ataque infeccioso de virus, bacterias y parásitos. Precisamente, este tpo de comportamientos sociales son, junto a los simbólicos, una de las mayores incógnitas todavía de la arqueología prehistórica. A partr de dos patologías infecciosas bien reconocidas en las poblaciones del pasado, como son la tuberculosis y la brucelosis, se hará especial referencia al análisis de los comportamientos sociales y cuidados humanos. Se observará que la propagación de dichas enfermedades tene mucho que ver con la llegada de las innovaciones neolítcas al territorio europeo, suponiendo para diversas de estas afecciones un punto de partda y de persistencia en el tempo gracias a su prevalencia en el ser humano.
- Published
- 2022
50. Eficacia de la prueba Elisa en muestras de leche para la vigilancia epidemiológica de la brucelosis bovina
- Author
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Andrade Guzman, Omar Santiago, Vintimilla Rojas, Andrea Elizabeth, Guevara Viera, Guillermo Null, Dutan Sanango, Jorge Bolivar, Lupercio Novillo, Rosa Lucia, Bustamante Ordoñez, Jorge Gualberto, Rivera Pirela, Sergio Emiro, Lopez Espinoza, Mateo Damian, Andrade Guzman, Omar Santiago, Vintimilla Rojas, Andrea Elizabeth, Guevara Viera, Guillermo Null, Dutan Sanango, Jorge Bolivar, Lupercio Novillo, Rosa Lucia, Bustamante Ordoñez, Jorge Gualberto, Rivera Pirela, Sergio Emiro, and Lopez Espinoza, Mateo Damian
- Abstract
Bovine brucellosis is a worldwide zoonotic disease capable of infecting cattle for long periods of time. Some animals are asymptomatic, thus maintaining the disease in the herd. Cattle become infected after ingestion of contaminated milk, feed, water; also by contact with infected animals, uterine secretions or aborted fetuses and by vertical transmission. Little is known about the epidemiological situation of brucellosis in dairy herds in the province of Azuay. An initial step for the formulation of control and eradication programs would be the implementation of a surveillance system that allows characterizing the state of infection in dairy areas, and thus early detection of the entry of the disease into free herds. The purpose of this study was to evaluate the efficacy of the indirect ELISA test (IDEXX, Brucella tank milk) in milk pools as a tool to identify herds that have not been in contact with the disease, allowing the characterization of areas free of brucellosis.
- Published
- 2022
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