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Your search keyword '"Brydon, Lena"' showing total 43 results
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43 results on '"Brydon, Lena"'

42. The PDZ Protein Mupp1 Promotes Gi Coupling and Signaling of the Mt1 Melatonin Receptor.

Author

Guillaume, Jean-Luc, Daulat, Avais M., Maurice, Pascal, Angélique Levoye, Migaud, Martine, Brydon, Lena, Malpaux, Benolt, Borg-Capra, Catherine, and Jockers, Ralf

Subjects
*G proteins, *MEMBRANE proteins, *MICROBIAL genetics, *BACTERIOPHAGES, *GENETIC transduction
Abstract

Intracellular signaling events are often organized around PDZ (PSD-95lDrosophila Disc large/ZO-1 homology) domain-containing scaffolding proteins. The ubiquitously expressed multi-PDZ protein MUPP1, which is composed of 13 PDZ domains, has been shown to interact with multiple viral and cellular proteins and to play important roles in receptor targeting and trafficking. In this study, we show that MUPP1 binds to the G protein-coupled MT1 melatonin receptor and directly regulates its G1-dependent signal transduction. Structural determinants involved in this interaction are the PDZ10 domain of MUPP1 and the valine of the canonical class III PDZ domain binding motif DSV of the MT1 carboxyl terminus. This high affinity interaction (Kd ∼- 4 nM), which is independent of MT1 activation, occurs in the ovine pars tuberalis of the pituitary expressing both proteins endogenously. Although the disruption of the MT1/MUPP1 interaction has no effect on the subcellular localization, trafficking, or degradation of MT1, it destabilizes the interaction between MT1 and Gi and abolishes Grmediated signaling of MT1. Our findings highlight a previously unappreciated role of PDZ proteins in promoting G protein coupling to receptors. [ABSTRACT FROM AUTHOR]

Published
2008
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43. Self-esteem levels and cardiovascular and inflammatory responses to acute stress.

Author

O'Donnell K, Brydon L, Wright CE, and Steptoe A

Subjects
Adiposity, Analysis of Variance, Body Mass Index, Enzyme-Linked Immunosorbent Assay, Female, Hematocrit, Humans, Interleukin 1 Receptor Antagonist Protein blood, Interleukin-6 blood, Male, Neuroimmunomodulation, Neuropsychological Tests, Photic Stimulation, Regression Analysis, Speech, Time Factors, Tumor Necrosis Factor-alpha blood, Young Adult, Heart Rate physiology, Self Concept, Stress, Psychological immunology, Stress, Psychological physiopathology
Abstract

Acute mental stress tests have helped to clarify the pathways through which psychosocial factors are linked to disease risk. This methodology is now being used to investigate potentially protective psychosocial factors. We investigated whether global self-esteem might buffer cardiovascular and inflammatory responses to acute stress. One hundred and one students completed the Rosenberg Self-Esteem Scale. Heart rate and heart rate variability (HRV) were recorded for 5 min periods at baseline, during two mental stress tasks, (a speech and a color-word task) and 10, 25 and 40 min into a recovery period. Plasma levels of tumor-necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 receptor antagonist (IL-1Ra) were assessed at baseline, immediately post-stress and after 45 min recovery. Repeated measures analysis of variance demonstrated that heart rate levels were lower across all time points in those with high self-esteem, although heart rate reactivity to stress was not related to self-esteem. There were no differences in baseline HRV, TNF-alpha, IL-6 or IL-1Ra. Multiple linear regressions revealed that greater self-esteem was associated with a smaller reduction in heart rate variability during the speech task, but not the color-word task. Greater self-esteem was associated with smaller TNF-alpha and IL-1Ra responses immediately following acute stress and smaller IL-1Ra responses at 45 min post-stress. In conclusion, global self-esteem is associated with lower heart rate and attenuated HRV and inflammatory responses to acute stress. These responses could be processes through which self-esteem protects against the development of disease.

Published
2008
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