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PurposeRemote ischemic preconditioning (RIPC) is a method of protection against induced ischemia reperfusion injury, and an increasing number of studies showed some of its inconclusive ergogenic effects in sports. RIPC involves short cycles of cuff inflation followed by its deflation which may affect many body systems. While most of the studies focus on single RIPC effects, there is insufficient data regarding training-like repeated RIPC interventions. Thus, in this study, we analyzed the effect of a single- and consecutive 10-day RIPC procedure on a single leg, focusing on the exerkine levels and changes in inflammation markers following the Wingate Anaerobic Test (WAnT).MethodsTwo single-blinded, sham-controlled protocols were designed to evaluate the 1) single (crossover study) and 2) consecutive 10-day (parallel study) RIPC effects on the WAnT performance and exercise-induced lactate, glucose, exerkine, and inflammation markers (BDNF; IL-6; IL-10; IL-15; LIF; oncostatin M). In each protocol, 37 physically active men (19.98 ± 1.17 years) were randomly assigned into two groups according to a particular study design.ResultsAn increase in participants’ mean (4.81%, p < 0.05) and peak power (6.25%, p < 0.05) during the WAnT was observed only after the consecutive 10-day RIPC. Similarly, a significant 15.5% (p < 0.05) decrease in the IL-6 concentration 120 min after the WAnT was observed only in the consecutive 10-day RIPC protocol, as well as a 12.2% (p < 0.01) increase in oncostatin M 60 min after the WAnT.ConclusionThe results demonstrate the efficacy of the consecutive 10-day RIPC procedure in modulating exercise performance and post-exercise inflammation markers.

Background Technosoils in Inowrocław, central Poland, are impacted by human activities and exhibit high salinity (ECe up to 70 dS/m) due to a soda lime repository. These saline environments pose challenges to plant growth and soil health. However, they also offer an opportunity for the evolution of microorganisms adapted to such conditions, including plant growth-promoting rhizospheric (PGPR) bacteria. The hypothesis tested here was that introducing PGPR bacteria from these environments could boost degraded soil performance, leading to better plant biomass and improved pathogen defense. Methods Azotobacter chroococcum W4ii was isolated from the rhizosphere of wheat (Triticum aestivum L.) for its plant growth properties on wheat plants under salt stress. Results Wheat seeds co-inoculated with A. chroococcum W4ii under 200 mM salt stress showed significant improvement in various growth parameters such as seeds germination (by 130%), shoot biomass (15%), chlorophyll b content (40%) compared to un-inoculated ones. Bacterial inoculation decreased the level of malondialdehyde (MDA) by 55.5% (P

Abstract In ovarian cancer, there is no current method to accurately predict recurrence after a complete response to chemotherapy. Here, we develop a machine learning risk score using serum proteomics for the prediction of early recurrence of ovarian cancer after initial treatment. The developed risk score was validated in an independent cohort with serum collected prospectively during the remission period. In the discovery cohort, patients scored as low-risk had a median time to recurrence (TTR) that was not reached at 10 years compared to 10.5 months (HR 4.66, p

Background Technosoils in Inowrocław, central Poland, are impacted by human activities and exhibit high salinity (ECe up to 70 dS/m) due to a soda lime repository. These saline environments pose challenges to plant growth and soil health. However, they also offer an opportunity for the evolution of microorganisms adapted to such conditions, including plant growth-promoting rhizospheric (PGPR) bacteria. The hypothesis tested here was that introducing PGPR bacteria from these environments could boost degraded soil performance, leading to better plant biomass and improved pathogen defense. Methods Azotobacter chroococcum W4ii was isolated from the rhizosphere of wheat (Triticum aestivum L.) for its plant growth properties on wheat plants under salt stress. Results Wheat seeds co-inoculated with A. chroococcum W4ii under 200 mM salt stress showed significant improvement in various growth parameters such as seeds germination (by 130%), shoot biomass (15%), chlorophyll b content (40%) compared to un-inoculated ones. Bacterial inoculation decreased the level of malondialdehyde (MDA), whereas it elevated the antioxidative enzymatic activities of peroxidase (POD). The test isolate also enhanced the level of defense enzymes like β-1,3-glucanase, which can protect plants from infection by pathogens. The bacterium could also successfully colonize the wheat plants. Conclusions These results indicate that A. chroococcum isolated from the technosoil has the potential to promote wheat growth under salt stress and can be further used as a bioinoculant in the salt affected agricultural fields.

Abstract Background Neutrophils are non-dividing cells with poor survival after isolation. Consequently, exogenous gene expression in neutrophils is challenging. We report here the transfection of genes and expression of active proteins in human primary peripheral neutrophils using nucleofection. Results Exogenous gene expression in human neutrophils was achieved 2 h post-transfection. We show that neutrophils transfected by nucleofection are functional cells, able to respond to soluble and particulate stimuli. They conserved the ability to undergo physiological processes including phagocytosis. Using this technique, we were able to show that the phox homology (PX) domain of p47phox localizes to the plasma membrane in human neutrophils. We also show that RhoB, but not the PX domain of p47phox, is translocated to the membrane of mature phagosomes. Conclusion We demonstrated that cDNA transfer and expression of exogenous protein in human neutrophils is compatible with cell viability and is no longer a limitation for the study of protein function in human neutrophils.

Even though canola is one of the most important industrial crops worldwide, it has high nutrient requirements and is susceptible to pests and diseases. Therefore, natural methods are sought to support the development of these plants. One of those methods could be a plant growth–promoting rhizobacteria (PGPR) that have a beneficial effect on plant development. The aim of this study was a genomic comparison of two PGPR strains chosen based on their effect on canola growth: Peribacillus frigoritolerans 2RO30, which stimulated canola growth only in sterile conditions, and Pseudomonas sivasensis 2RO45, which promoted canola growth in both sterile and non-sterile conditions. First of all, six bacterial strains: RO33 (Pseudomonas sp.), RO37 (Pseudomonas poae), RO45 (Pseudomonas kairouanensis), 2RO30 (Peribacillus frigoritolerans), 2RO45 (Pseudomonas sivasensis), and 3RO30 (Pseudomonas migulae), demonstrating best PGP traits in vitro, were studied for their stimulating effect on canola growth under sterile conditions. P. frigoritolerans 2RO30 and P. sivasensis 2RO45 showed the best promoting effect, significantly improving chlorophyll content index (CCI) and roots length compared to the non-inoculated control and to other inoculated seedlings. Under non-sterile conditions, only P. sivasensis 2RO45 promoted the canola growth, significantly increasing CCI compared to the untreated control and to other inoculants. Genome comparison revealed that the genome of P. sivasensis 2RO45 was enriched with additional genes responsible for ACC deaminase (acdA), IAA (trpF, trpG), and siderophores production (fbpA, mbtH, and acrB) compared to 2RO30. Moreover, P. sivasensis 2RO45 showed antifungal effect against all the tested phytopathogens and harbored six more biosynthetic gene clusters (BGC), namely, syringomycin, pyoverdin, viscosin, arylpolyene, lankacidin C, and enterobactin, than P. frigoritolerans 2RO30. These BGCs are well known as antifungal agents; therefore, it can be assumed that these BGCs were responsible for the antifungal activity of P. sivasensis 2RO45 against all plant pathogens. This study is the first report describing P. sivasensis 2RO45 as a canola growth promoter, both under controlled and natural conditions, thus suggesting its application in improving canola yield, by improving nutrient availability, enhancing stress tolerance, and reducing environmental impact of farming practices.

BackgroundThe Occlutech Atrial Flow Regulator (AFR) is a self-expandable double-disc nitinol device with a central fenestration. Its use has been approved in the adult population with heart failure and described for pulmonary hypertension (PH). Only case reports and small series have been published about its use in the paediatric population and for congenital heart disease (CHD).ObjectivesThe authors sought to investigate the feasibility, safety, and short-term follow-up of AFR implantation in patients with CHD or children with PH or cardiomyopathy.MethodsThis is a multicenter retrospective study involving 10 centers worldwide. Patients of any age with CHD or patients aged