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A study was conducted to examine the role of single alanine substitution at the Bacillus subtilis transcription factor SpoOA on the activity of spoIIG promoters. The amino acid substitution in SpoOA which suppressed the phenotype of the H359R substitution in sigmaA subunit of the RNA polymerase showed that H359R substitution on sigmaA prevented its interaction with SpoOA. Results suggested the relevance of the region of SpoOA near position 229 in sigmaA-dependent promoter activation.

The significance of the interaction of Spo0A and the sigma subunit of RNA polymerase in the activation of the Bacillus subtilis spoIIG promoter was analyzed. Results of the study revealed that B. subtilis Spo0A activates form both sigmaA-and-sigmaH dependent promoters. Alanine substitutions at these positions were discovered to be significantly reduced from the sigmaA-dependent, Spo0A-dependent promoters, spoIIG and spoIIE in vivo.

Cites a study which examined the effects of single alanine substitutions, in an attempt to determine SpoOA activities in the bacterium Bacillus subtilis. What SpoOA is; Isolation of the derivatives of sigH mutants of SpoOA; Results of the study.

Examines the transcription from both o-A and O-H dependent promoters activated by Bacillus subtilis SpoOA. Identification of two amino acid substitutions in the carboxyl terminus of o-A, K356E and H359R; Effect of alanine substitutions in o-A and on o-A's ability to direct transcription in vivo and in vitro.