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8 results on '"Budziar, Wiktoria"'

2. Immunogenic epitope scanning in bacteriolytic enzymes Pal and Cpl-1 and engineering Pal to escape antibody responses

Author

Harhala, Marek Adam, primary, Gembara, Katarzyna, additional, Rybicka, Izabela, additional, Kaźmierczak, Zuzanna Maria, additional, Miernikiewicz, Paulina, additional, Majewska, Joanna Marta, additional, Budziar, Wiktoria, additional, Nasulewicz-Goldeman, Anna, additional, Nelson, Daniel C., additional, Owczarek, Barbara, additional, and Dąbrowska, Krystyna, additional

Published
2023
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3. Escaping antibody responses to bacteriolytic enzymes Pal and Cpl-1 by epitope scanning and engineering

Author

Harhala, Marek, primary, Gembara, Katarzyna, additional, Rybicka, Izabela, additional, Kaźmierczak, Zuzanna, additional, Miernikiewicz, Paulina, additional, Majewska, Joanna, additional, Budziar, Wiktoria, additional, Nasulewicz-Goldeman, Anna, additional, Nelson, Daniel C., additional, Owczarek, Barbara, additional, and Dąbrowska, Krystyna, additional

Published
2022
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4. Immunogenic epitope scanning in bacteriolytic enzymes Pal and Cpl-1 and engineering Pal to escape antibody responses.

Author

Adam Harhala, Marek, Gembara, Katarzyna, Rybicka, Izabela, Kaźamierczak, Zuzanna Maria, Miernikiewicz, Paulina, Majewska, Joanna Marta, Budziar, Wiktoria, Nasulewicz-Goldeman, Anna, Nelson, Daniel C., Owczarek, Barbara, and Dabrowska, Krystyna

Subjects
STARTLE reaction, ANTIBODY formation, NEUTRALIZATION tests, ENZYMES, IMMUNE response, ANTIBACTERIAL agents
Abstract

Bacteriolytic enzymes are promising antibacterial agents, but they can cause a typical immune response in vivo. In this study, we used a targeted modification method for two antibacterial endolysins, Pal and Cpl-1. We identified the key immunogenic amino acids, and designed and tested new, bacteriolytic variants with altered immunogenicity. One new variant of Pal (257-259 MKS → TFG) demonstrated decreased immunogenicity while a similar mutant (257-259 MKS → TFK) demonstrated increased immunogenicity. A third variant (280-282 DKP →GGA) demonstrated significantly increased antibacterial activity and it was not cross-neutralized by antibodies induced by the wild-type enzyme. We propose this variant as a new engineered endolysin with increased antibacterial activity that is capable of escaping cross-neutralization by antibodies induced by wild-type Pal. We show that efficient antibacterial enzymes that avoid cross-neutralization by IgG can be developed by epitope scanning, in silico design, and substitutions of identified key amino acids with a high rate of success. Importantly, this universal approach can be applied to many proteins beyond endolysins and has the potential for design of numerous biological drugs. [ABSTRACT FROM AUTHOR]

Published
2023
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5. Dynamics of anti-SARS-CoV-2 seroconversion in individual patients and at the population level

Author

Szewczyk-Dąbrowska, Alina, primary, Budziar, Wiktoria, additional, Baniecki, Krzysztof, additional, Pikies, Aleksandra, additional, Harhala, Marek, additional, Jędruchniewicz, Natalia, additional, Kaźmierczak, Zuzanna, additional, Gembara, Katarzyna, additional, Klimek, Tomasz, additional, Witkiewicz, Wojciech, additional, Nahorecki, Artur, additional, Barczyk, Kamil, additional, Grata-Borkowska, Urszula, additional, and Dąbrowska, Krystyna, additional

Published
2022
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6. Correlation between COVID-19 severity and previous exposure of patients to Borrelia spp.

Author

Szewczyk-Dabrowska, Alina, primary, Budziar, Wiktoria, additional, Harhala, Marek, additional, Baniecki, Krzysztof, additional, Pikies, Aleksandra, additional, Jedruchniewicz, Natalia, additional, Kazmierczak, Zuzanna, additional, Gembara, Katarzyna, additional, Klimek, Tomasz, additional, Witkiewicz, Wojciech, additional, Nahorecki, Artur, additional, Barczyk, Kamil, additional, Klak, Marlena, additional, Grata-Borkowska, Urszula, additional, and Dabrowska, Krystyna, additional

Published
2022
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7. Hidden fraction of Polish population immune to SARS-CoV-2 in May 2021

Author

Budziar, Wiktoria, primary, Gembara, Katarzyna, additional, Harhala, Marek, additional, Szymczak, Aleksander, additional, Jędruchniewicz, Natalia, additional, Baniecki, Krzysztof, additional, Pikies, Aleksandra, additional, Nahorecki, Artur, additional, Hoffmann, Agnieszka, additional, Kardaś, Amelia, additional, Szewczyk-Dąbrowska, Alina, additional, Klimek, Tomasz, additional, Kaźmierczak, Zuzanna, additional, Witkiewicz, Wojciech, additional, Barczyk, Kamil, additional, and Dąbrowska, Krystyna, additional

Published
2022
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8. Hidden fraction of Polish population immune to SARS-CoV-2 in May 2021

Author

Budziar, Wiktoria, primary, Gembara, Katarzyna, additional, Szymczak, Aleksander, additional, Jędruchniewicz, Natalia, additional, Baniecki, Krzysztof, additional, Pikies, Aleksandra, additional, Nahorecki, Artur, additional, Hoffmann, Agnieszka, additional, Kardaś, Amelia, additional, Klimek, Tomasz, additional, Kaźmierczak, Zuzanna, additional, Witkiewicz, Wojciech, additional, Barczyk, Kamil, additional, and Dąbrowska, Krystyna, additional

Published
2021
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