Search

Under semiarid and warm climates, field practices for climate change adaptation have to be defined in order to modulate grape composition according to the desired wine styles. Under this context, the present study investigated several viticulture practices in cv. Macabeo for Cava production. The experiment was carried out over 3 years in a commercial vineyard located in the province of Valencia (eastern Spain). The techniques tested were (i) vine shading, (ii) double pruning (bud forcing), and (iii) the combined application of soil organic mulching and shading, all of them tested against a control. Double pruning significantly modified phenology and grape composition, improving the wine alcohol-to-acidity ratio and reducing the pH. Similar results were also achieved by shading. However, the shading strategy did not significantly affect yield, unlike double pruning, which reduced vine yield even in the year following its application. Shading alone or in combination with mulching significantly improved the vine water status, suggesting that these techniques can also be used to alleviate water stress. Particularly, we found that the effect of soil organic mulching and canopy shading on stem water potential was additive. Indeed, all the techniques tested were useful for improving wine composition for cava production, but double pruning is only recommended for premium Cava production.

Because of climate change and the scarce availability of natural resources there is a need to develop sustainable intensification strategies intended for optimizing water use in vineyards. In this study, water regime, fertilization and soil management practices were assessed in terms of vineyard water use, by evaluating the inter-row and crop line evapotranspiration (ET) components using the Mapping EvapoTranspiration at high Resolution with Internalized Calibration (METRIC) model in combination with unmanned aerial vehicle multispectral and thermal images taken on five dates throughout the growing season. The application of the METRIC-UAV using high-resolution imagery was proven as a useful tool for evaluating the effects of sustainable intensification strategies on water use of crops where vegetation does not completely cover the soil, identifying the most efficient site-specific strategies for water conservation purposes. Moreover, METRIC-UAV allowed evaluating separately their effects on the inter-row and the crop line. Among the assessed sustainable intensification strategies, the application of mulching provided the highest water savings (- 28%) when compared to traditional soil tillage management, reducing inter-row soil evaporation by 63%, while increasing crop-line ET by 14%. In spite of this, the mulching application did not affect yield, but significantly enhanced water use efficiency (WUE) in terms of grape yield compared to tillage. The adoption of deficit irrigation (DI) strategies did not result in vine water stress that was severe enough to significantly affect crop line ET when compared with fully irrigated (FI) vines. Both DI and FI strategies increased vine water use by 18% and 27%, respectively, as compared to the rainfed regime, with no differences found in the inter-row water consumption. DI and FI, in turn, significantly increased yield as compared to rainfed crops, leading to significant improvements in WUE. In the short term, the application

The use of more salt stress-tolerant vine rootstocks can be a sustainable strategy for adapting traditional grapevine cultivars to future conditions. However, how the new M1 and M4 rootstocks perform against salinity compared to conventional ones, such as the 1103-Paulsen, had not been previously assessed under real field conditions. Therefore, a field trial was carried out in a young ‘Tempranillo’ (Vitis vinifera L.) vineyard grafted onto all three rootstocks under a semi-arid and hot-summer Mediterranean climate. The vines were irrigated with two kinds of water: a non-saline Control with EC of 0.8 dS m–1 and a Saline treatment with 3.5 dS m–1. Then, various physiological parameters were assessed in the scion, and, additionally, gene expression was studied by high throughput sequencing in leaf and berry tissues. Plant water relations evidenced the osmotic effect of water quality, but not that of the rootstock. Accordingly, leaf-level gas exchange rates were also reduced in all three rootstocks, with M1 inducing significantly lower net photosynthesis rates than 1103-Paulsen. Nevertheless, the expression of groups of genes involved in photosynthesis and amino acid metabolism pathways were not significantly and differentially expressed. The irrigation with saline water significantly increased leaf chloride contents in the scion onto the M-rootstocks, but not onto the 1103P. The limitation for leaf Cl– and Na+ accumulation on the scion was conferred by rootstock. Few processes were differentially regulated in the scion in response to the saline treatment, mainly, in the groups of genes involved in the flavonoids and phenylpropanoids metabolic pathways. However, these transcriptomic effects were not fully reflected in grape phenolic ripeness, with M4 being the only one that did not cause reductions in these compounds in response to salinity, and 1103-Paulsen having the highest overall concentrations. These results suggest that all three rootstocks confer short-term sali

The use of more salt stress-tolerant vine rootstocks can be a sustainable strategy for adapting traditional grapevine cultivars to future conditions. However, how the new M1 and M4 rootstocks perform against salinity compared to conventional ones, such as the 1103-Paulsen, had not been previously assessed under real field conditions. Therefore, a field trial was carried out in a young ‘Tempranillo’ (Vitis vinifera L.) vineyard grafted onto all three rootstocks under a semi-arid and hot-summer Mediterranean climate. The vines were irrigated with two kinds of water: a non-saline Control with EC of 0.8 dS m–1 and a Saline treatment with 3.5 dS m–1. Then, various physiological parameters were assessed in the scion, and, additionally, gene expression was studied by high throughput sequencing in leaf and berry tissues. Plant water relations evidenced the osmotic effect of water quality, but not that of the rootstock. Accordingly, leaf-level gas exchange rates were also reduced in all three rootstocks, with M1 inducing significantly lower net photosynthesis rates than 1103-Paulsen. Nevertheless, the expression of groups of genes involved in photosynthesis and amino acid metabolism pathways were not significantly and differentially expressed. The irrigation with saline water significantly increased leaf chloride contents in the scion onto the M-rootstocks, but not onto the 1103P. The limitation for leaf Cl– and Na+ accumulation on the scion was conferred by rootstock. Few processes were differentially regulated in the scion in response to the saline treatment, mainly, in the groups of genes involved in the flavonoids and phenylpropanoids metabolic pathways. However, these transcriptomic effects were not fully reflected in grape phenolic ripeness, with M4 being the only one that did not cause reductions in these compounds in response to salinity, and 1103-Paulsen having the highest overall concentrations. These results suggest that all three rootstocks confer short-term sali

Warm and semi-arid climates are characterized by rainfall scarcity, resulting in the frequent use of low-quality water for irrigation. This work was undertaken to study the effects of water stress and saline irrigation on yield and grape composition of Monastrell grapevines grafted onto 1103P rootstock. The experiment was carried out during three consecutive seasons in a commercial vineyard located in Jumilla (SE Spain) with a loamy-sandy soil. Rainfed vines were compared with five watering regimes including a Control, irrigated with standard water, and four treatments that combined two different schedules for irrigation initiation (pre- and post-veraison) with saline water obtained by adding two types of salts (sulphates and chlorides). Vines from treatments with more severe water stress (i.e., rainfed) showed lower yields and vegetative growth. Moreover, the Rainfed treatment clearly modified grape composition when compared with the Control treatment by increasing berry phenolic content. The application of saline water slightly affected vine performance and grape composition regardless of the type of salts added to the irrigation water. Indeed, the watering regime had a greater effect on yield, vegetative growth and grape composition than the use of different saline waters. Our results suggest that, in the mid-term (3 years), and with a vineyard soil with good drainage, the use of saline waters is not detrimental to vine performance, but does not improve grape composition. Further research is required to assess the long-term effects of saline water application, particularly in view of the important accumulation of chlorides and sodium in leaf tissues observed in vines watered with salty water at the last season of this experiment.

The use of more salt stress-tolerant vine rootstocks can be a sustainable strategy for adapting traditional grapevine cultivars to future conditions. However, how the new M1 and M4 rootstocks perform against salinity compared to conventional ones, such as the 1103-Paulsen, had not been previously assessed under real field conditions. Therefore, a field trial was carried out in a young ‘Tempranillo’ (Vitis vinifera L.) vineyard grafted onto all three rootstocks under a semi-arid and hot-summer Mediterranean climate. The vines were irrigated with two kinds of water: a non-saline Control with EC of 0.8 dS m–1 and a Saline treatment with 3.5 dS m–1. Then, various physiological parameters were assessed in the scion, and, additionally, gene expression was studied by high throughput sequencing in leaf and berry tissues. Plant water relations evidenced the osmotic effect of water quality, but not that of the rootstock. Accordingly, leaf-level gas exchange rates were also reduced in all three rootstocks, with M1 inducing significantly lower net photosynthesis rates than 1103-Paulsen. Nevertheless, the expression of groups of genes involved in photosynthesis and amino acid metabolism pathways were not significantly and differentially expressed. The irrigation with saline water significantly increased leaf chloride contents in the scion onto the M-rootstocks, but not onto the 1103P. The limitation for leaf Cl– and Na+ accumulation on the scion was conferred by rootstock. Few processes were differentially regulated in the scion in response to the saline treatment, mainly, in the groups of genes involved in the flavonoids and phenylpropanoids metabolic pathways. However, these transcriptomic effects were not fully reflected in grape phenolic ripeness, with M4 being the only one that did not cause reductions in these compounds in response to salinity, and 1103-Paulsen having the highest overall concentrations. These results suggest that all three rootstocks confer short-term sali

The sustainability of woody crop agroecosystems requires continued improvements to water, nutrient and soil management. In this work, the combination of resource availability or fertigation dose with soil management practices was tested in a 2 × 2 factorial design in a drip irrigated young almond orchard. The fertigation doses were: the farmer supply at 60% of crop evapotranspiration and full nutrient requirements and the half-farmer supply at 30% of crop evapotranspiration and half nutrient requirements. The soil management practices were: bare soil and cover crops in the inter-row (mixture of grasses and legumes). Tree growth, yield, water and nutrient status, and cover crop biomass and nutrient status were determined, as well as inter-row soil C and N concentration. Results showed that the effect of resource availability was independent of soil management and vice versa. The half farmer treatment reduced tree vegetative growth and yield compared to farmer treatment, due to a negative effect on the water status, without observing a decrease in the concentration of nutrients in leaves or fruit. Trees with cover crop also reduced growth and yield compared to bare soil management. This was due to a nutritional competition, mainly of Ca, Fe, Mn and Zn, rather than to a decline in tree water status. Cover crops sequester up to 1 t/ha/year of carbon but do not increase soil organic carbon, nor soil total nitrogen. Cover crops proved to be efficient in reducing soil nitrate concentration in the topsoil and therefore has potential to prevent its leaching. Deficit fertigation and the use of cover crops can be effective practices to preserve and save water and nutrient resources in Mediterranean agroecosystems, but should be established with caution so as not to compromise the profitability of the orchard.

Optimizing water use in vineyards is crucial for ensuring the sustainability of viticulture in semi-arid regions, and this may be achieved by minimizing direct water evaporation from the soil through the use of mulching. In this context, the current study aimed at assessing the combined effects of the vine-row application of an organic mulch (vine prunings) and no-tillage under two water regimes on soil properties, plant water and nutritional status, yield and must composition of grapevine (Vitis vinifera L.) cv. Bobal grown under semi-arid conditions. For this purpose, a field experiment in a split-plot design was carried out for three years (2016–2018) in a mature Bobal vineyard located in Eastern Spain. Two soil management strategies (tillage and organic mulching with no-tillage) were assessed under two water regimes (rainfed and deficit drip irrigation) with four replications per combination. Vine responses were determined by measuring midday stem water potential, leaf nutrient concentrations, pruning weight, yield components and grape composition. Soil properties were assessed at the end of the experiment. Mulching and no-tillage positively affected vine water status under both water regimes, resulting in reductions in grape phenolic composition. Interactive effects of both water regime and soil management on water use efficiency were found. Regardless of soil management practice, irrigation increased yield and pruning weight when compared to rainfed conditions. Soil management had slight effects on vine nutritional status. At the end of the experiment, soil compaction increased and infiltration decreased as a consequence of mulching and no-tillage. Organic mulch and no-tillage improved vine water status, however, considering the final soil surface compaction and low water infiltration rate, longer-term studies are necessary to assess the sustainability of combining both practices.

Irrigation effects in relation to the environmental conditions on grape aromas are still unknown. This study aims to clarify the effects of water regime on the aromatic composition of “Muscat of Alexandria” grapes under the semiarid climate conditions of eastern Spain and over three seasons. The relationships between total volatile composition in free and glycosidically-bound fractions, vine performance, and grape composition were also assessed. The watering treatments studied were: sustained deficit irrigation (SDI) at 50% of the estimated crop evapotranspiration (ET); early deficit (EDI), where pre-veraison water deficit was imposed; late deficit (LDI), in which a water shortage was applied during post-veraison; and control (C), irrigated at 100% of ET during the entire season. The effects of water regimes on volatile and glycosidically-bound composition were different between seasons due to the predominant effect of the environmental conditions on aromatic composition. The seasons with the greater yield were associated to a lower grape aromatic composition, however, this effect at the crop level was less pronounced in the most irrigated treatment. In drier seasons, irrigation delayed ripening and increased the terpene concentration of “Muscat of Alexandria” grapes, showing a high treatment and season interaction. Specific water regimes have the potential to buffer the effects of environmental conditions on the aromatic composition of “Muscat of Alexandria” grapes in a semi-arid climate, as the result of the complex relationships between climatic factors, vine performance and grape maturity.

Background and Aims: Climate change is advancing grapevine phenology, decoupling berry technological and phenolic maturity. The aim of this study was to assess the usefulness of late winter pruning for delaying grape ripening. Methods and Results: In two Bobal and Tempranillo vineyards located in eastern Spain, vines pruned at dormancy were compared with a delayed winter pruning (late pruning) carried out just before the basal buds burst (BBCH 05). In both cultivars, late pruning delayed grape ripening, resulting in grapes with higher anthocyanin concentration for similar TSS at harvest. In general, the resulting wines had a higher colour intensity and lower hue angle. These effects were more noticeable in Tempranillo, an earlier ripening cultivar, than in Bobal. Despite late pruning improving vine water status, the yield was steadily reduced by 10%. Conclusions: Late pruning is a useful strategy for delaying grape ripening, thus adapting grapevine production to climate change. The small detrimental effects on yield and compression of pruning dates within a few days may limit the application of this practice. Significance of the Study: Delaying winter pruning can be used to improve grape and wine composition when facing changes in the meteorological conditions experienced during berry ripening.

This study tested the possibility of improving whole-canopy water use efficiency (WUE) of grapevines (cv. Bobal) by maximizing radiation interception during the mornings and limiting this during the afternoons, when the vapour pressure deficit and the evaporative demand are higher. The three-year study consisted of two trials conducted in parallel on North-South row oriented potted- and field-grown grapevines. In both trials, performance in terms of vine water use, yield and WUE in a vertical shoot positioned (VSP) system was compared with that of vines leaned 30° towards West (WSP). Potted vines were fully irrigated, whereas field-grown vines were submitted to rain-fed and deficit irrigation conditions. In potted plants, there was no difference in daily transpiration between vines from the WSP and VSP treatments, but transpiration in the mornings was higher in WSP vines. Dry matter and berry size increased in WSP compared to VSP vines. In the field, watering regime had a greater effect than canopy inclination on vine performance. Nonetheless, the WSP system increased leaf area by 13%, yield by 12% and WUE by 11% compared to VSP, although differences in WUE were not statistically significant and the effect on yield was negligible under rain-fed conditions. In both trials, the WSP system did not have a major effect on grape composition (soluble solids, pH, total acidity, concentrations of anthocyanins and polyphenols). In conclusion, this pioneering three-year study proved that leaning vine canopies to the West increased grapevine performance despite the great effect that environmental conditions exerted each year on the data obtained. Further research is required to study the effects of different patterns of light interception on carbon balance and grape biochemical composition.

The relation between water-use and intercepted solar radiation depends on many factors involved in vine canopy architecture and physiology. In addition, vine productivity is related to the efficiency with which the intercepted photosynthetically active radiation (IPAR) is used, which in turn depends mainly on water availability and transport. In hedgerow-managed vines it exists the possibility to modulate IPAR by orienting their rows, influencing water-use efficiency (WUE), defined as dry matter produced by water used. Aiming to unravel the effects of row orientation on WUE, a three-year experiment was carried out in Valencia (Spain) on potted Vitis vinifera (L.) cv. Bobal and Verdejo with vine rows oriented either north-south (NS) or east-west (EW), under no-water restrictions. Simulated radiation interception over the growing seasons at the experimental plot showed an average 39% reduction in daily IPAR when EW was compared to NS. Vine transpiration was quantified by water balance, decreasing by 16% in Bobal and 8% in Verdejo when comparing EW against NS. In both cultivars, this reduction was 18% when considered relative to the total leaf area. Carbon assimilation was not markedly affected by row orientation. Therefore, since in both cultivars minor differences in vine performance occurred between orientations, WUE tended to increase by orienting the rows to the EW compared to NS. This resulted in most of the seasons an increase in water productivity calculated as grape yield/water-use ratio. Leaf gas exchange measurements partially agreed with the radiation interception simulations, suggesting a more complex regulatory mechanism and highlighting the importance of canopy microclimatic conditions in the physiological processes of hedgerow-managed crops. These findings encourage further research under field conditions and different soil water availabilities, aiming to optimize grapevine water productivity.