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Abstract A one year active surveillance program for influenza A viruses among avian species in a live-bird market (LBM) in Bangkok, Thailand was conducted in 2009. Out of 970 samples collected, influenza A virus subtypes H4N6 (n = 2) and H4N9 (n = 1) were isolated from healthy Muscovy ducks. All three viruses were characterized by whole genome sequencing with subsequent phylogenetic analysis and genetic comparison. Phylogenetic analysis of all eight viral genes showed that the viruses clustered in the Eurasian lineage of influenza A viruses. Genetic analysis showed that H4N6 and H4N9 viruses display low pathogenic avian influenza characteristics. The HA cleavage site and receptor binding sites were conserved and resembled to LPAI viruses. This study is the first to report isolation of H4N6 and H4N9 viruses from birds in LBM in Thailand and shows the genetic diversity of the viruses circulating in the LBM. In addition, co-infection of H4N6 and H4N9 in the same Muscovy duck was observed.

Abstract In January and November 2008, outbreaks of avian influenza have been reported in 4 provinces of Thailand. Eight Influenza A H5N1 viruses were recovered from these 2008 AI outbreaks and comprehensively characterized and analyzed for nucleotide identity, genetic relatedness, virulence determinants, and possible sites of reassortment. The results show that the 2008 H5N1 viruses displayed genetic drift characteristics (less than 3% genetic differences), as commonly found in influenza A viruses. Based on phylogenetic analysis, clade 1 viruses in Thailand were divided into 3 distinct branches (subclades 1, 1.1 and 1.2). Six out of 8 H5N1 isolates have been identified as reassorted H5N1 viruses, while other isolates belong to an original H5N1 clade. These viruses have undergone inter-lineage reassortment between subclades 1.1 and 1.2 and thus represent new reassorted 2008 H5N1 viruses. The reassorted viruses have acquired gene segments from H5N1, subclade 1.1 (PA, HA, NP and M) and subclade 1.2 (PB2, PB1, NA and NS) in Thailand. Bootscan analysis of concatenated whole genome sequences of the 2008 H5N1 viruses supported the reassortment sites between subclade 1.1 and 1.2 viruses. Based on estimating of the time of the most recent common ancestors of the 2008 H5N1 viruses, the potential point of genetic reassortment of the viruses could be traced back to 2006. Genetic analysis of the 2008 H5N1 viruses has shown that most virulence determinants in all 8 genes of the viruses have remained unchanged. In summary, two predominant H5N1 lineages were circulating in 2008. The original CUK2-like lineage mainly circulated in central Thailand and the reassorted lineage (subclades 1.1 and 1.2) predominantly circulated in lower-north Thailand. To prevent new reassortment, emphasis should be put on prevention of H5N1 viruses circulating in high risk areas. In addition, surveillance and whole genome sequencing of H5N1 viruses should be routinely performed for monitoring the genetic drift of the virus and new reassorted strains, especially in light of potential reassortment between avian and mammalian H5N1 viruses.

To investigate an outbreak of African horse sickness (AHS) on a horse farm in northeastern Thailand, we used whole-genome sequencing to detect and characterize the virus. The viruses belonged to [...]

Norovirus infection is a major cause of endemic and epidemic acute gastroenteritis. These viruses have been classified into 7 genogroups on the basis of the major capsid protein, VP1. Noroviruses [...]

Influenza A(H9N2) virus has been found in several avian species. Despite low pathogenicity in poultry, H9N2 viruses are important to public health because of their high adaptability and frequent infection [...]

In April 2009, a novel swine origin influenza A (H1N1) virus, now referred to as pandemic (H1N1) 2009 virus, emerged in humans in Mexico and the United States and spread [...]

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has caused the coronavirus disease 2019 (COVID‐19) pandemic in humans since late 2019. Here, we investigated SARS‐CoV‐2 infection in dogs and cats during COVID‐19 quarantine at private veterinary hospitals in Thailand. From April to May 2021, we detected SARS‐CoV‐2 in three out of 35 dogs and one out of nine cats from four out of 17 households with confirmed COVID‐19 patients. SARS‐CoV‐2 RNA was detected from one of the nasal, oral, rectal and environmental swabs of dog‐A (15 years old, mixed breed, male dog), cat‐B (1 year old, domestic shorthair, male cat), dog‐C (2 years old, mixed breed, female dog) and dog‐D (4 years old, Pomeranian, female dog). The animals tested positive for SARS‐CoV‐2 RNA from 4 to 30 days after pet owners were confirmed to be COVID‐19 positive. The animals consecutively tested positive for SARS‐CoV‐2 RNA for 4 to 10 days. One dog (dog‐A) showed mild clinical signs, while the other dogs and a cat remained asymptomatic during quarantine at the hospitals. SARS‐CoV‐2 specific neutralizing antibodies were detected in both the dogs and cat by surrogate virus neutralization tests. Phylogenetic and genomic mutation analyses of whole genome sequences of three SARS‐CoV‐2 strains from the dogs and cat revealed SARS‐CoV‐2 of the Alpha variant (B.1.1.7 lineage). Our findings are suggestive of human‐to‐animal transmission of SARS‐CoV‐2 in COVID‐19‐positive households and contamination of viral RNA in the environment. Public awareness of SARS‐CoV‐2 infection in pet dogs and cats in close contact with COVID‐19 patients should be raised. [ABSTRACT FROM AUTHOR]

Coronavirus disease of 2019 (COVID‐19) caused by severe acute respiratory syndrome virus type 2 (SARS‐CoV‐2) is an emerging severe acute respiratory disease affecting global human health. In this study, a large‐scale serological survey of antibodies against SARS‐CoV‐2 in dogs and cats was conducted during the first and second waves of COVID‐19 outbreaks in Thailand, from April to December 2020. A total of 3215 serum samples were collected from dogs (n = 2102) and cats (n = 1113) living in Bangkok and in the vicinities. Serum samples were tested for SARS‐CoV‐2 antibodies by using an indirect multispecies enzyme‐linked immunosorbent assay (ELISA). Positive and suspected samples were additionally tested for neutralizing antibodies by the surrogate virus neutralization test (sVNT). The indirect ELISA results showed that 1.66% (35 out of 2103) of dogs and 0.36% (four out of 1112) of cats were positive for SARS‐CoV‐2 antibodies. The sVNT results showed that all ELISA‐positive and suspected samples were negative for neutralizing antibodies. Positive serum samples (35 dogs and four cats) were obtained from clinically healthy animals and animals with mild respiratory signs aged <1–13 years living in Bangkok and Samutprakarn Provinces. In summary, a serological survey revealed evidence of anti‐N‐IgG antibodies suggesting SARS‐CoV‐2 exposure in both dogs and cats during the first and second COVID‐19 outbreaks in Thailand. [ABSTRACT FROM AUTHOR]

From September 2016 to January 2019, we collected 710 rectal swabs from both healthy and sick dogs from small animal hospitals in 5 provinces of Thailand. The samples were tested for canine rotavirus group A (CRV) by using one‐step RT‐PCR specific to the VP6 gene. Our results showed that 0.70% (5/710) were positive for CRV. The five CRVs were then characterized by whole‐genome sequencing. Our results showed that the genotype of Thai CRVs is G3P[3], which is the predominant genotype reported in dogs. The Thai CRVs posed a novel genetic constellation 'G3‐P[3]‐I3‐R3‐C3‐M3‐A9‐N2‐T3‐E3‐H6', which has never been reported in CRVs from dogs but has been reported in rotaviruses from humans. Based on phylogenetic analysis, the Thai CRVs are the result of multiple reassortments in which gene segments might have originated from human and bat rotaviruses and suggests the zoonotic potential of the virus. [ABSTRACT FROM AUTHOR]

Influenza A virus causes respiratory disease in both humans and animals. In this study, a survey of influenza A antibodies in domestic dogs and cats was conducted in 47 animal shelters in 19 provinces of Thailand from September 2011 to September 2014. One thousand and eleven serum samples were collected from 932 dogs and 79 cats. Serum samples were tested for influenza A antibodies using a multi‐species competitive NP‐ELISA and haemagglutination inhibition (HI) assay. The NP‐ELISA results showed that 0.97% (9/932) of dogs were positive, but all cat samples were negative. The HI test against pandemic H1N1, human H3N2 and canine H3N2 showed that 0.64% (6/932) and 1.20% (1/79) of dogs and cats were positive, respectively. It is noted that all six serum samples (5 dogs and 1 cat) had antibodies against pandemic H1N1. In summary, a serological survey revealed the evidence of pandemic H1N1 influenza exposure in both dogs and cats in the shelters in Thailand. [ABSTRACT FROM AUTHOR]

Objectives: This study aimed to identify occupational risk factors for brucellosis among small scale goat farmers in Thailand. Methods: To better understand farmers' knowledge, attitudes and practices associated with brucellosis we interviewed 51 farmers and tested 314 goats for Brucella melitensis. Results: All serological samples tested negative for Brucella infection. Based on previous research and estimates provided from the Thai national brucellosis surveillance system, zero seropositivity was less than expected. Findings from interviews with farmers demonstrate that most respondents were relatively new to goat farming with just over half (53%) reporting owning goats for five or fewer years. The majority of respondents demonstrated important gaps in knowledge on disease transmission in animals and knowledge of human brucellosis was particularly limited with just over half (54%) reporting that humans could become infected. Participants had a very low perceived risk of infection with the majority (91.7%) reporting that they or a member of their household were not at risk of the disease. Conclusion: Overall findings from this study support that brucellosis poses an occupational risk to goat farmers with specific areas of concern including weak awareness of disease transmission to humans and lack of knowledge on specific safe farm practices such as quarantine practices. Findings from this study contribute to a more comprehensive understanding of brucellosis in Thailand by identifying specific occupational risk factors and describing areas where farmer education and training should be strengthened. [ABSTRACT FROM AUTHOR]

Routine surveillance of influenza A virus was conducted in Myanmar during 2014-2015. Influenza A(H9N2) virus was isolated in Shan State, upper Myanmar. Whole-genome sequencing showed that H9N2 virus from Myanmar was closely related to H9N2 virus of clade 4.2.5 from China. [ABSTRACT FROM AUTHOR]

Background and Aim: Free-grazing duck (FGD) raising is a unique domestic duck production system that is widely practiced in several Asian countries, including Thailand. FGD is a significant reservoir for influenza A viruses (IAVs). In this study, we genetically characterized IAV-H10N6 and IAV-H10N7 isolated from avian influenza surveillance in FGDs in Thailand. Materials and Methods: We collected 640 swab samples from 29 FGD flocks located in 6 provinces of Thailand. IAVs were isolated from swab samples using egg inoculation. Hemagglutination test-positive samples were then subjected to IAV detection. Viral RNA was subjected to IAV detection using real-time reverse-transcription polymerase chain reaction (rRT-PCR) specific to matrix (M) gene. IAV subtypes were identified using the RT-PCR assay specific to all hemagglutinin and neuraminidase subtypes. Whole-genome sequencing of IAVs was performed to genetically characterize IAV-H10N6 and IAV-H10N7. Results: Our results showed that 41 (6.41%) samples tested positive for IAV using rRT-PCR specific to the M gene. Among these, only two IAVs were subtypes as IAV-H10N6 and IAV-H10N7 and were subjected to whole-genome sequencing. IAV-H10N6 and IAV-H10N7 belonged to the Eurasian lineage and did not show any evidence of reassortment from the North American lineage. The viruses exhibited low-pathogenic characteristics and preferred binding to avian-type receptors. Genetic analysis revealed no mutations in PB2 and M genes, unlike human IAV-H10N3 and IAV-H10N8, which exhibited increased virulence in mammals. Conclusion: IAV-H10N6 and IAV-H10N7 viruses have less potential as zoonotic viruses. However, IAV in FGDs should be monitored for novel reassortant or zoonotic viruses. This study provides information on the genetic characteristics and diversity of IAV-H10N6 and IAV-H10N7 that are circulated in FGDs in Thailand. [ABSTRACT FROM AUTHOR]