158 results on '"Burrello, J."'
Search Results
2. Machine learning applied to ambulatory blood pressure monitoring: a new tool to diagnose autonomic failure?
- Author
-
Vallelonga, Fabrizio, Sobrero, G., Merola, A., Valente, M., Giudici, M., Di Stefano, C., Milazzo, V., Burrello, J., Burrello, A., Veglio, F., Romagnolo, A., and Maule, S.
- Published
- 2022
- Full Text
- View/download PDF
3. Sphingolipid composition of circulating extracellular vesicles after myocardial ischemia
- Author
-
Burrello, J., Biemmi, V., Dei Cas, M., Amongero, M., Bolis, S., Lazzarini, E., Bollini, S., Vassalli, G., Paroni, R., and Barile, L.
- Published
- 2020
- Full Text
- View/download PDF
4. Familial hyperaldosteronism type III
- Author
-
Monticone, S, Tetti, M, Burrello, J, Buffolo, F, De Giovanni, R, Veglio, F, Williams, T A, and Mulatero, P
- Published
- 2017
- Full Text
- View/download PDF
5. A dynamic clamping approach using in silico IK1 current for discrimination of chamber-specific hiPSC-derived cardiomyocytes
- Author
-
Altomare, C, Bartolucci, C, Sala, L, Balbi, C, Burrello, J, Pietrogiovanna, N, Burrello, A, Bolis, S, Panella, S, Arici, M, Krause, R, Rocchetti, M, Severi, S, Barile, L, Altomare, Claudia, Bartolucci, Chiara, Sala, Luca, Balbi, Carolina, Burrello, Jacopo, Pietrogiovanna, Nicole, Burrello, Alessio, Bolis, Sara, Panella, Stefano, Arici, Martina, Krause, Rolf, Rocchetti, Marcella, Severi, Stefano, Barile, Lucio, Altomare, C, Bartolucci, C, Sala, L, Balbi, C, Burrello, J, Pietrogiovanna, N, Burrello, A, Bolis, S, Panella, S, Arici, M, Krause, R, Rocchetti, M, Severi, S, Barile, L, Altomare, Claudia, Bartolucci, Chiara, Sala, Luca, Balbi, Carolina, Burrello, Jacopo, Pietrogiovanna, Nicole, Burrello, Alessio, Bolis, Sara, Panella, Stefano, Arici, Martina, Krause, Rolf, Rocchetti, Marcella, Severi, Stefano, and Barile, Lucio
- Abstract
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CM) constitute a mixed population of ventricular-, atrial-, nodal-like cells, limiting the reliability for studying chamber-specific disease mechanisms. Previous studies characterised CM phenotype based on action potential (AP) morphology, but the classification criteria were still undefined. Our aim was to use in silico models to develop an automated approach for discriminating the electrophysiological differences between hiPSC-CM. We propose the dynamic clamp (DC) technique with the injection of a specific IK1 current as a tool for deriving nine electrical biomarkers and blindly classifying differentiated CM. An unsupervised learning algorithm was applied to discriminate CM phenotypes and principal component analysis was used to visualise cell clustering. Pharmacological validation was performed by specific ion channel blocker and receptor agonist. The proposed approach improves the translational relevance of the hiPSC-CM model for studying mechanisms underlying inherited or acquired atrial arrhythmias in human CM, and for screening anti-arrhythmic agents.
- Published
- 2023
6. COMPARISON OF AUTOMATED OFFICE BLOOD PRESSURE WITH OFFICE AND OUT-OF-OFFICE MEASUREMENT TECHNIQUES: A SYSTEMATIC REVIEW AND META-ANALYSIS
- Author
-
Pappaccogli, M., Di Monaco, S., Perlo, E., Burrello, J., D’Ascenzo, F., Veglio, F., Monticone, S., and Rabbia, F.
- Published
- 2019
- Full Text
- View/download PDF
7. CHARACTERIZATION AND GENE EXPRESSION ANALYSIS OF SERUM-DERIVED EXTRACELLULAR VESICLES IN PRIMARY ALDOSTERONISM
- Author
-
Burrello, J., Gai, C., Tetti, M., Lopatina, T., Deregibus, M.C., Veglio, F., Mulatero, P., Camussi, G., and Monticone, S.
- Published
- 2019
- Full Text
- View/download PDF
8. C25 PREDICTION OF ALL–CAUSE MORTALITY FOLLOWING PERCUTANEOUS CORONARY INTERVENTION IN BIFURCATION LESIONS USING MACHINE LEARNING ALGORITHMS – THE RAIN–ML PREDICTION MODEL
- Author
-
Gallone, G, primary, Burrello, J, additional, Burrello, A, additional, Iannaccone, M, additional, De Luca, L, additional, Patti, G, additional, Cerrato, E, additional, Venuti, G, additional, De Filippo, O, additional, Mattesini, A, additional, Muscoli, S, additional, Trabattoni, D, additional, Giammaria, M, additional, Truffa, A, additional, Cortese, B, additional, Conrotto, F, additional, Mulatero, P, additional, Monticone, S, additional, Escaned, J, additional, Usmiani, T, additional, D‘ascenzo, F, additional, De Ferrari, G, additional, and Breviario, S, additional
- Published
- 2022
- Full Text
- View/download PDF
9. Inflammatory extracellular vesicles prompt heart dysfunction via TRL4-dependent NF-κB activation
- Author
-
Biemmi, V., Milano, G., Ciullo, A., Cervio, E., Burrello, J., Dei Cas, M., Paroni, R., Tallone, T., Moccetti, T., Pedrazzini, G., Longnus, S., Vassalli, G., Barile, L., University of Zurich, and Barile, Lucio
- Subjects
Inflammation ,Male ,TLR4 axis ,inflammatory extracellular vesicles ,macrophages ,myocardial infarction ,Inflammatory extracellular vesicles ,Macrophages ,Myocardial infarction ,Myocardium ,Troponin I ,NF-kappa B ,2701 Medicine (miscellaneous) ,610 Medicine & health ,11171 Cardiocentro Ticino ,Rats ,Toll-Like Receptor 4 ,Extracellular Vesicles ,Animals, Newborn ,Animals ,Cytokines ,Myocytes, Cardiac ,3001 Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Rats, Wistar ,Cells, Cultured ,Research Paper - Abstract
Background: After myocardial infarction, necrotic cardiomyocytes release damage-associated proteins that stimulate innate immune pathways and macrophage tissue infiltration, which drives inflammation and myocardial remodeling. Circulating inflammatory extracellular vesicles play a crucial role in the acute and chronic phases of ischemia, in terms of inflammatory progression. In this study, we hypothesize that the paracrine effect mediated by these vesicles induces direct cytotoxicity in cardiomyocytes. Thus, we examined whether reducing the generation of inflammatory vesicles within the first few hours after the ischemic event ameliorates cardiac outcome at short and long time points. Methods: Myocardial infarction was induced in rats that were previously injected intraperitoneally with a chemical inhibitor of extracellular-vesicle biogenesis. Heart global function was assessed by echocardiography performed at 7, 14 and 28 days after MI. Cardiac outcome was also evaluated by hemodynamic analysis at sacrifice. Cytotoxic effects of circulating EV were evaluated ex-vivo in a Langendorff, system by measuring the level of cardiac troponin I (cTnI) in the perfusate. Mechanisms undergoing cytotoxic effects of EV derived from pro-inflammatory macrophages (M1) were studied in-vitro in primary rat neonatal cardiomyocytes. Results: Inflammatory response following myocardial infarction dramatically increased the number of circulating extracellular vesicles carrying alarmins such as IL-1α, IL-1β and Rantes. Reducing the boost in inflammatory vesicles during the acute phase of ischemia resulted in preserved left ventricular ejection fraction in vivo. Hemodynamic analysis confirmed functional recovery by displaying higher velocity of left ventricular relaxation and improved contractility. When added to the perfusate of isolated hearts, post-infarction circulating vesicles induced significantly more cell death in adult cardiomyocytes, as assessed by cTnI release, comparing to circulating vesicles isolated from healthy (non-infarcted) rats. In vitro inflammatory extracellular vesicles induce cell death by driving nuclear translocation of NF-κB into nuclei of cardiomyocytes. Conclusion: Our data suggest that targeting circulating extracellular vesicles during the acute phase of myocardial infarction may offer an effective therapeutic approach to preserve function of ischemic heart.
- Published
- 2020
10. The Primary Aldosteronism Surgical Outcome Score for the Prediction of Clinical Outcomes After Adrenalectomy for Unilateral Primary Aldosteronism
- Author
-
Burrello, J., Burrello, A., Stowasser, M., Nishikawa, T., Quinkler, M., Prejbisz, A., Lenders, J.W.M., Satoh, F., Mulatero, P., Reincke, M., Williams, T.A., Burrello, J., Burrello, A., Stowasser, M., Nishikawa, T., Quinkler, M., Prejbisz, A., Lenders, J.W.M., Satoh, F., Mulatero, P., Reincke, M., and Williams, T.A.
- Abstract
Contains fulltext : 229517.pdf (Publisher’s version ) (Closed access), OBJECTIVE: To develop a prediction model for clinical outcomes after unilateral adrenalectomy for unilateral primary aldosteronism. SUMMARY BACKGROUND DATA: Unilateral primary aldosteronism is the most common surgically curable form of endocrine hypertension. Surgical resection of the dominant overactive adrenal in unilateral primary aldosteronism results in complete clinical success with resolution of hypertension without antihypertensive medication in less than half of patients with a wide between-center variability. METHODS: A linear discriminant analysis model was built using data of 380 patients treated by adrenalectomy for unilateral primary aldosteronism to classify postsurgical clinical outcomes. The total cohort was then randomly divided into training (280 patients) and test (100 patients) datasets to create and validate a score system to predict clinical outcomes. An online tool (Primary Aldosteronism Surgical Outcome predictor) was developed to facilitate the use of the predictive score. RESULTS: Six presurgical factors associated with complete clinical success (known duration of hypertension, sex, antihypertensive medication dosage, body mass index, target organ damage, and size of largest nodule at imaging) were selected based on classification performance in the linear discriminant analysis model. A 25-point predictive score was built with an optimal cut-off of greater than 16 points (accuracy of prediction = 79.2%; specificity = 84.4%; sensitivity = 71.3%) with an area under the curve of 0.839. CONCLUSIONS: The predictive score and the primary aldosteronism surgical outcome predictor can be used in a clinical setting to differentiate patients who are likely to be clinically cured after surgery from those who will need continuous surveillance after surgery due to persistent hypertension.
- Published
- 2020
11. Use of Steroid Profiling Combined With Machine Learning for Identification and Subtype Classification in Primary Aldosteronism
- Author
-
Eisenhofer, G., Durán, C., Cannistraci, C.V., Peitzsch, M., Williams, T.A., Riester, A., Burrello, J., Buffolo, F., Prejbisz, A., Beuschlein, F., Januszewicz, A., Mulatero, P., Lenders, J.W.M., Reincke, M., Eisenhofer, G., Durán, C., Cannistraci, C.V., Peitzsch, M., Williams, T.A., Riester, A., Burrello, J., Buffolo, F., Prejbisz, A., Beuschlein, F., Januszewicz, A., Mulatero, P., Lenders, J.W.M., and Reincke, M.
- Abstract
Contains fulltext : 225820.pdf (publisher's version ) (Open Access), IMPORTANCE: Most patients with primary aldosteronism, a major cause of secondary hypertension, are not identified or appropriately treated because of difficulties in diagnosis and subtype classification. Applications of artificial intelligence combined with mass spectrometry-based steroid profiling could address this problem. OBJECTIVE: To assess whether plasma steroid profiling combined with machine learning might facilitate diagnosis and treatment stratification of primary aldosteronism, particularly for patients with unilateral adenomas due to pathogenic KCNJ5 sequence variants. DESIGN, SETTING, AND PARTICIPANTS: This diagnostic study was conducted at multiple tertiary care referral centers. Steroid profiles were measured from June 2013 to March 2017 in 462 patients tested for primary aldosteronism and 201 patients with hypertension. Data analyses were performed from September 2018 to August 2019. MAIN OUTCOMES AND MEASURES: The aldosterone to renin ratio and saline infusion tests were used to diagnose primary aldosteronism. Subtyping was done by adrenal venous sampling and follow-up of patients who underwent adrenalectomy. Statistical tests and machine-learning algorithms were applied to plasma steroid profiles. Areas under receiver operating characteristic curves, sensitivity, specificity, and other diagnostic performance measures were calculated. RESULTS: Primary aldosteronism was confirmed in 273 patients (165 men [60%]; mean [SD] age, 51 [10] years), including 134 with bilateral disease and 139 with unilateral adenomas (58 with and 81 without somatic KCNJ5 sequence variants). Plasma steroid profiles varied according to disease subtype and were particularly distinctive in patients with adenomas due to KCNJ5 variants, who showed better rates of biochemical cure after adrenalectomy than other patients. Among patients tested for primary aldosteronism, a selection of 8 steroids in combination with the aldosterone to renin ratio showed improved effectiveness for d
- Published
- 2020
12. Sphingolipid composition of circulating extracellular vesicles after myocardial ischemia
- Author
-
Burrello, J, Biemmi, V, Dei Cas, M, Amongero, M, Bolis, S, Lazzarini, E, Bollini, S, Vassalli, G, Paroni, R, Barile, L, Burrello, J, Biemmi, V, Dei Cas, M, Amongero, M, Bolis, S, Lazzarini, E, Bollini, S, Vassalli, G, Paroni, R, and Barile, L
- Abstract
Sphingolipids are structural components of cell membrane, displaying several functions in cell signalling. Extracellular vesicles (EV) are lipid bilayer membrane nanoparticle and their lipid composition may be different from parental cells, with a significant enrichment in sphingolipid species, especially in pathological conditions. We aimed at optimizing EV isolation from plasma and describing the differential lipid content of EV, as compared to whole plasma. As pilot study, we evaluated the diagnostic potential of lipidomic signature of circulating EV in patients with a diagnosis of ST-segment-elevation myocardial infarction (STEMI). STEMI patients were evaluated before reperfusion and 24-h after primary percutaneous coronary intervention. Twenty sphingolipid species were quantified by liquid-chromatography tandem-mass-spectrometry. EV-ceramides, -dihydroceramides, and -sphingomyelins increased in STEMI vs. matched controls and decreased after reperfusion. Their levels correlated to hs-troponin, leucocyte count, and ejection fraction. Plasma sphingolipids levels were 500-to-700-fold higher as compared to EV content; nevertheless, only sphingomyelins differed in STEMI vs. control patients. Different sphingolipid species were enriched in EV and their linear combination by machine learning algorithms accurately classified STEMI patients at pre-PCI evaluation. In conclusion, EV lipid signature discriminates STEMI patients. These findings may contribute to the identification of novel biomarkers and signaling mechanisms related to cardiac ischemia.
- Published
- 2020
13. Poster session: Dobutamine stress echo
- Author
-
Tosello, F, Milan, A, Magnino, C, Leone, D, Chiarlo, M, Bruno, G, Losano, I, Burrello, J, Fulcheri, C, and Veglio, F
- Published
- 2012
14. Classification of microadenomas in patients with primary aldosteronism by steroid profiling
- Author
-
Yang, Yuhong, Burrello, J., Burrello, Alessio, Eisenhofer, G., Peitzsch, M., Tetti, Martina, Lenders, J.W.M., Reincke, M., Williams, T.A., Yang, Yuhong, Burrello, J., Burrello, Alessio, Eisenhofer, G., Peitzsch, M., Tetti, Martina, Lenders, J.W.M., Reincke, M., and Williams, T.A.
- Abstract
Contains fulltext : 203987.pdf (publisher's version ) (Open Access)
- Published
- 2019
15. PHARMACOLOGICAL TREATMENT OF ARTERIAL HYPERTENSION IN CHILDREN AND ADOLESCENTS
- Author
-
Burrello, J., primary, Erhardt, E.M., additional, Saint-Hilary, G., additional, Forestiero, V., additional, Veglio, F., additional, Rabbia, F., additional, Mulatero, P., additional, Monticone, S., additional, and D’Ascenzo, F., additional
- Published
- 2019
- Full Text
- View/download PDF
16. IMPROVING HYPERTENSION CONTROL BY MOLECULAR STRATIFICATION OF FIRST-LINE NON-RESPONDERS USING RAAS TRIPLE-A TESTING
- Author
-
Poglitsch, M., primary, Ahmed, A.H., additional, Guo, Z., additional, Burrello, J., additional, Stoller, A., additional, Burkard, T., additional, Domenig, O., additional, Haschke, M., additional, Mulatero, P., additional, and Stowasser, M., additional
- Published
- 2019
- Full Text
- View/download PDF
17. Immunohistopathology and Steroid Profiles Associated With Biochemical Outcomes After Adrenalectomy for Unilateral Primary Aldosteronism
- Author
-
Meyer, Lucie S., Wang, Xiao, Susnik, Eva, Burrello, J., Burrello, Alessio, Castellano, Isabella, Lenders, J.W.M., Reincke, M., Williams, T.A., Meyer, Lucie S., Wang, Xiao, Susnik, Eva, Burrello, J., Burrello, Alessio, Castellano, Isabella, Lenders, J.W.M., Reincke, M., and Williams, T.A.
- Abstract
Item does not contain fulltext
- Published
- 2018
18. Outcomes after adrenalectomy for unilateral primary aldosteronism: an international consensus on outcome measures and analysis of remission rates in an international cohort
- Author
-
Williams, T.A., Lenders, J.W.M., Mulatero, P., Burrello, J., Rottenkolber, M., Adolf, C., Satoh, F., Amar, L., Quinkler, M., Deinum, J., Beuschlein, F., Kitamoto, K.K., Pham, U., Morimoto, R., Umakoshi, H., Prejbisz, A., Kocjan, T., Naruse, M., Stowasser, M., Nishikawa, T., Young, W.F., Jr., Gomez-Sanchez, C.E., Funder, J.W., Reincke, M., Williams, T.A., Lenders, J.W.M., Mulatero, P., Burrello, J., Rottenkolber, M., Adolf, C., Satoh, F., Amar, L., Quinkler, M., Deinum, J., Beuschlein, F., Kitamoto, K.K., Pham, U., Morimoto, R., Umakoshi, H., Prejbisz, A., Kocjan, T., Naruse, M., Stowasser, M., Nishikawa, T., Young, W.F., Jr., Gomez-Sanchez, C.E., Funder, J.W., and Reincke, M.
- Abstract
Item does not contain fulltext, BACKGROUND: Although unilateral primary aldosteronism is the most common surgically correctable cause of hypertension, no standard criteria exist to classify surgical outcomes. We aimed to create consensus criteria for clinical and biochemical outcomes and follow-up of adrenalectomy for unilateral primary aldosteronism and apply these criteria to an international cohort to analyse the frequency of remission and identify preoperative determinants of successful outcome. METHODS: The Primary Aldosteronism Surgical Outcome (PASO) study was an international project to develop consensus criteria for outcomes and follow-up of adrenalectomy for unilateral primary aldosteronism. An international panel of 31 experts from 28 centres, including six endocrine surgeons, used the Delphi method to reach consensus. We then retrospectively analysed follow-up data from prospective cohorts for outcome assessment of patients diagnosed with unilateral primary aldosteronism by adrenal venous sampling who had undergone a total adrenalectomy, consecutively included from 12 referral centres in nine countries. On the basis of standardised criteria, we determined the proportions of patients achieving complete, partial, or absent clinical and biochemical success in accordance with the consensus. We then used logistic regression analyses to identify preoperative factors associated with clinical and biochemical outcomes. FINDINGS: Consensus was reached for criteria for six outcomes (complete, partial, and absent success of clinical and biochemical outcomes) based on blood pressure, use of antihypertensive drugs, plasma potassium and aldosterone concentrations, and plasma renin concentrations or activities. Consensus was also reached for two recommendations for the timing of follow-up assessment. For the international cohort analysis, we analysed clinical data from 705 patients recruited between 1994 and 2015, of whom 699 also had biochemical data. Complete clinical success was achieved in 259 (37
- Published
- 2017
19. [PP.27.23] GUIDELINES FOR PRIMARY ALDOSTERONISM
- Author
-
Monticone, S., primary, Mulatero, P., additional, Burrello, J., additional, Veglio, F., additional, Williams, T. A., additional, and Funder, J. W., additional
- Published
- 2017
- Full Text
- View/download PDF
20. [BP.04.05] PREVALENCE AND CLINICAL PHENOTYPE OF PRIMARY ALDOSTERONISM IN PRIMARY CARE HYPERTENSIVES
- Author
-
Monticone, S., primary, Burrello, J., additional, Tizzani, D., additional, Bertello, C., additional, Viola, A., additional, Buffolo, F., additional, Williams, T.A., additional, Rabbia, F., additional, Veglio, F., additional, and Mulatero, P., additional
- Published
- 2017
- Full Text
- View/download PDF
21. [PP.25.07] EVOLUTION OF COMPUTED TOMOGRAPHY-DETECTABLE ADRENAL NODULES IN PATIENTS WITH BILATERAL PRIMARY ALDOSTERONISM
- Author
-
Mulatero, P., primary, Burrello, J., additional, Lucatello, B., additional, Monticone, S., additional, Giacchetti, G., additional, Ronconi, V., additional, Battocchio, M., additional, and Fallo, F., additional
- Published
- 2016
- Full Text
- View/download PDF
22. KCNJ5 Mutations: Sex, Salt and Selection
- Author
-
Williams, T.A., Lenders, J.W.M., Burrello, J., Beuschlein, F., Reincke, M., Williams, T.A., Lenders, J.W.M., Burrello, J., Beuschlein, F., and Reincke, M.
- Abstract
Item does not contain fulltext, Somatic mutations have been identified in the KCNJ5 gene (encoding the potassium channel GIRK4) in aldosterone-producing adenomas (APA). Most of these mutations are located in or near the selectivity filter of the GIRK4 channel pore and several have been shown to lead to the constitutive overproduction of aldosterone. KCNJ5 mutations in APA are more frequent in women; however, this gender dimorphism is a reported phenomenon of Western but not East Asian populations. In this review we discuss some of the issues that could potentially underlie this observation.
- Published
- 2015
23. Subtype Diagnosis of Primary Aldosteronism: Approach to Different Clinical Scenarios
- Author
-
Burrello, J., additional, Monticone, S., additional, Tetti, M., additional, Rossato, D., additional, Versace, K., additional, Castellano, I., additional, Williams, T., additional, Veglio, F., additional, and Mulatero, P., additional
- Published
- 2015
- Full Text
- View/download PDF
24. KCNJ5 Mutations: Sex, Salt and Selection
- Author
-
Lenders, J. W. M., primary, Burrello, J., primary, Beuschlein, F., primary, Reincke, M., primary, and Williams, T. A., additional
- Published
- 2015
- Full Text
- View/download PDF
25. 9B.06
- Author
-
Burrello, J., primary, Buffolo, F., additional, Monticone, S., additional, Viola, A., additional, Falcetta, A., additional, Lucchiari, M., additional, Mengozzi, G., additional, Rabbia, F., additional, Veglio, F., additional, and Mulatero, P., additional
- Published
- 2015
- Full Text
- View/download PDF
26. LB02.08
- Author
-
Caprino, M. Parasiliti, primary, Lucatello, B., additional, Bima, C., additional, D’Angelo, V., additional, Burrello, J., additional, Piovesan, A., additional, La Grotta, A., additional, Giordano, R., additional, Mulatero, P., additional, Veglio, F., additional, Arvat, E., additional, Ghigo, E., additional, and Maccario, M., additional
- Published
- 2015
- Full Text
- View/download PDF
27. PP.09.26
- Author
-
Grillo, A., primary, Bernardi, S., additional, Rebellato, A., additional, Fabris, B., additional, Bardelli, M., additional, Burrello, J., additional, Rabbia, F., additional, Veglio, F., additional, Fallo, F., additional, and Carretta, R., additional
- Published
- 2015
- Full Text
- View/download PDF
28. Renin and Aldosterone Measurements in the Management of Arterial Hypertension
- Author
-
Viola, A., additional, Monticone, S., additional, Burrello, J., additional, Buffolo, F., additional, Lucchiari, M., additional, Rabbia, F., additional, Williams, T., additional, Veglio, F., additional, Mengozzi, G., additional, and Mulatero, P., additional
- Published
- 2015
- Full Text
- View/download PDF
29. KCNJ5 Mutations: Sex, Salt and Selection.
- Author
-
Williams, T. A., Lenders, J. W. M., Burrello, J., Beuschlein, F., and Reincke, M.
- Subjects
SOMATIC cells ,GENETIC mutation ,ALDOSTERONE ,ADENOMA ,SEXUAL dimorphism - Abstract
Somatic mutations have been identified in the KCNJ5 gene (encoding the potassium channel GIRK4) in aldosterone-producing adenomas (APA). Most of these mutations are located in or near the selectivity filter of the GIRK4 channel pore and several have been shown to lead to the constitutive overproduction of aldosterone. KCNJ5 mutations in APA are more frequent in women; however, this gender dimorphism is a reported phenomenon of Western but not East Asian populations. In this review we discuss some of the issues that could potentially underlie this observation. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
30. Primary Aldosteronism: Who Should be Screened?
- Author
-
Monticone, S., additional, Viola, A., additional, Tizzani, D., additional, Crudo, V., additional, Burrello, J., additional, Galmozzi, M., additional, Veglio, F., additional, and Mulatero, P., additional
- Published
- 2011
- Full Text
- View/download PDF
31. CONCURRENT PRIMARY ALDOSTERONISM AND SUBCLINICAL CORTISOL HYPERSECRETION. A PERSPECTIVE STUDY
- Author
-
Fallo, F., primary, Bertello, C., additional, Tizzani, D., additional, Sonino, N., additional, Fassina, A., additional, Zennaro, M. C., additional, Covella, M., additional, Berra, E., additional, Crudo, V., additional, Burrello, J., additional, Leone, D., additional, Veglio, F., additional, and Mulatero, P., additional
- Published
- 2011
- Full Text
- View/download PDF
32. ALDOSTERONE DOES NOT MODIFY GENE EXPRESSION IN HUMAN ENDOTHELIAL CELLS
- Author
-
Verhovez, A., primary, Williams, T. A., additional, Crudo, V., additional, Burrello, J., additional, Covella, M., additional, Leone, D., additional, Berra, E., additional, Fallo, F., additional, Fabris, B., additional, Amenta, F., additional, Gomez-Sanchez, C. E., additional, Veglio, F., additional, and Mulatero, P., additional
- Published
- 2011
- Full Text
- View/download PDF
33. PSYCHOLOGICAL ASSESSMENT OF PRIMARY ALDOSTERONISM. A CONTROLLED STUDY
- Author
-
Sonino, N., primary, Tomba, E., additional, Genesia, M. L., additional, Bertello, C., additional, Crudo, V., additional, Burrello, J., additional, Covella, M., additional, Berra, E., additional, Leone, D., additional, Mulatero, P., additional, Veglio, F., additional, Fava, G. A., additional, and Fallo, F., additional
- Published
- 2011
- Full Text
- View/download PDF
34. Primary Aldosteronism: Who Should be Screened?
- Author
-
Monticone, S., Viola, A., Tizzani, D., Crudo, V., Burrello, J., Galmozzi, M., Veglio, F., and Mulatero, P.
- Subjects
HYPERALDOSTERONISM ,PATIENTS ,HYPERTENSION ,ALDOSTERONE ,ADENOMA ,DRUG resistance ,DIURETICS ,METABOLIC syndrome ,THERAPEUTICS - Abstract
Primary aldosteronism (PA) has a prevalence in the general hypertensive population from 5 to 10%, and is widely recognized as the most frequent form of secondary hypertension. The 2 main PA subtypes are aldosterone producing adenoma (APA) and bilateral adrenal hyperplasia (BAH) that account for 95% of all PA cases. The diagnosis of PA is a 3-step process that comprises screening, confirmatory testing, and subtype differentiation. The different categories of patients at an increased risk of PA who should thus undergo a screening test were described in the first Endocrine Society (ES) Practice Guidelines for diagnosis and treatment of PA published in 2008. These categories include patients with Joint National Committee Stage 2, Stage 3, or drug-resistant hypertension; hypertension, and spontaneous or diuretic-induced hypokalemia; hypertension with adrenal incidentaloma; hypertension and a family history of early-onset hypertension or cerebrovascular accident at a young age and all hypertensive first degree relatives of patients with PA. Recently, a growing number of studies have linked PA with the metabolic syndrome, diabetes, and obstructive sleep apnea that may be partly responsible for the higher rate of cardio and cerobrovascular accidents in PA patients. The aim of this review is to discuss, which patients should be screened for PA, focusing not only on the well-established categories of the ES Guidelines, but also on additional other group of patients with a potentially high prevalence of PA that has emerged from recent research. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
35. A dynamic clamping approach using in silico IK1 current for discrimination of chamber-specific hiPSC-derived cardiomyocytes
- Author
-
Claudia Altomare, Chiara Bartolucci, Luca Sala, Carolina Balbi, Jacopo Burrello, Nicole Pietrogiovanna, Alessio Burrello, Sara Bolis, Stefano Panella, Martina Arici, Rolf Krause, Marcella Rocchetti, Stefano Severi, Lucio Barile, Altomare, C, Bartolucci, C, Sala, L, Balbi, C, Burrello, J, Pietrogiovanna, N, Burrello, A, Bolis, S, Panella, S, Arici, M, Krause, R, Rocchetti, M, Severi, S, Barile, L, Altomare, Claudia, Bartolucci, Chiara, Sala, Luca, Balbi, Carolina, Burrello, Jacopo, Pietrogiovanna, Nicole, Burrello, Alessio, Bolis, Sara, Panella, Stefano, Arici, Martina, Krause, Rolf, Rocchetti, Marcella, Severi, Stefano, and Barile, Lucio
- Subjects
dynamic clamp, IK1, atrial myocytes, modeling, hiPSC ,Medicine (miscellaneous) ,dynamic clamping, hiPSC-derived cardiomyocytes, in silico IK1 current ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology - Abstract
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CM) constitute a mixed population of ventricular-, atrial-, nodal-like cells, limiting the reliability for studying chamber-specific disease mechanisms. Previous studies characterised CM phenotype based on action potential (AP) morphology, but the classification criteria were still undefined. Our aim was to use in silico models to develop an automated approach for discriminating the electrophysiological differences between hiPSC-CM. We propose the dynamic clamp (DC) technique with the injection of a specific IK1 current as a tool for deriving nine electrical biomarkers and blindly classifying differentiated CM. An unsupervised learning algorithm was applied to discriminate CM phenotypes and principal component analysis was used to visualise cell clustering. Pharmacological validation was performed by specific ion channel blocker and receptor agonist. The proposed approach improves the translational relevance of the hiPSC-CM model for studying mechanisms underlying inherited or acquired atrial arrhythmias in human CM, and for screening anti-arrhythmic agents.
- Published
- 2023
36. Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology.
- Author
-
Bonifay A, Mackman N, Hisada Y, Sachetto ATA, Hau C, Gray E, Hogwood J, Aharon A, Badimon L, Barile L, Baudar J, Beckmann L, Benedikter B, Bolis S, Bouriche T, Brambilla M, Burrello J, Camera M, Campello E, Ettelaie C, Faille D, Featherby S, Franco C, Guldenpfennig M, Hansen JB, Judicone C, Kim Y, Kristensen SR, Laakmann K, Langer F, Latysheva N, Lucien F, de Menezes EM, Mullier F, Norris P, Nybo J, Orbe J, Osterud B, Paramo JA, Radu CM, Roncal C, Samadi N, Snir O, Suades R, Wahlund C, Chareyre C, Abdili E, Martinod K, Thaler J, Dignat-George F, Nieuwland R, and Lacroix R
- Subjects
- Humans, Reproducibility of Results, Blood Coagulation, COVID-19 blood, COVID-19 diagnosis, COVID-19 immunology, Predictive Value of Tests, Thromboplastin metabolism, Extracellular Vesicles metabolism
- Abstract
Background: Scientific and clinical interest in extracellular vesicles (EVs) is growing. EVs that expose tissue factor (TF) bind factor VII/VIIa and can trigger coagulation. Highly procoagulant TF-exposing EVs are detectable in the circulation in various diseases, such as sepsis, COVID-19, or cancer. Many in-house and commercially available assays have been developed to measure EV-TF activity and antigen, but only a few studies have compared some of these assays., Objectives: The International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Subcommittee on Vascular Biology initiated a multicenter study to compare the sensitivity, specificity, and reproducibility of these assays., Methods: Platelet-depleted plasma samples were prepared from blood of healthy donors. The plasma samples were spiked either with EVs from human milk or EVs from TF-positive and TF-negative cell lines. Plasma was also prepared from whole human blood with or without lipopolysaccharide stimulation. Twenty-one laboratories measured EV-TF activity and antigen in the prepared samples using their own assays representing 18 functional and 9 antigenic assays., Results: There was a large variability in the absolute values for the different EV-TF activity and antigen assays. Activity assays had higher specificity and sensitivity compared with antigen assays. In addition, there was a large intra-assay and interassay variability. Functional assays that used a blocking anti-TF antibody or immunocapture were the most specific and sensitive. Activity assays that used immunocapture had a lower coefficient of variation compared with assays that isolated EVs by high-speed centrifugation., Conclusion: Based on this multicenter study, we recommend measuring EV-TF using a functional assay in the presence of an anti-TF antibody., Competing Interests: Declaration of competing interests F.D.-G. and R.L. filed a patent on microvesicle fibrinolytic activity licensed to Stago and obtained a common grant within the framework of the excellence program innovative tests to customize antiplatelet therapy in chronic kidney disease with acute coronary syndrome. The remaining authors declare no competing financial interests., (Copyright © 2024. Published by Elsevier Inc.)
- Published
- 2024
- Full Text
- View/download PDF
37. Management of Renovascular Hypertension and Renal Denervation in Patients with Hypertension: An Italian Nationwide Survey.
- Author
-
Pappaccogli M, Ponsa L, Goi J, Burrello J, Di Dalmazi G, Cicero AFG, Mancusi C, Moia EC, Iaccarino G, Borghi C, Muiesan ML, Ferri C, Rabbia F, and Mulatero P
- Abstract
Introduction: Renovascular hypertension (RVH) remains underdiagnosed despite its significant cardiovascular and renal morbidity., Aim: This survey investigated screening and management practices for RVH among hypertensive patients in Italian hypertension centres in a real-life setting. Secondary, we analysed the current spread of renal denervation (RDN) and the criteria used for its eligibility., Methods: A 12 item-questionnaire was sent to hypertension centres belonging to the European Society of Hypertension and to the Italian Society of Hypertension (SIIA) in Italy. Data concerning the screening and management of RVH and of RDN were analysed according to the type of centre (excellence vs non-excellence centres), geographical area and medical specialty., Results: Eighty-two centres participated to the survey. The number of patients diagnosed in each centre with RVH and fibromuscular dysplasia during the last five years was 3 [1;6] and 1 [0;2], respectively. Despite higher rates of RVH diagnosis in excellence centres (p = 0.017), overall numbers remained unacceptably low, when compared to expected prevalence estimates. Screening rates were inadequate, particularly among young hypertensive patients, with only 28% of the centres screening for RVH in such population. Renal duplex ultrasound was underused, with computed tomographic angiography or magnetic resonance angiography reserved for confirming a RVH diagnosis (76.8%) rather than for screening (1.9-32.7%, according to patients' characteristics). Scepticism and logistical challenges limited RDN widespread adoption., Conclusions: These findings underscore the need for improving RVH screening strategies and for a wider use of related diagnostic tools. Enhanced awareness and adherence to guidelines are crucial to identifying renovascular hypertension and mitigating associated cardiovascular and renal risks., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
38. Prevalence of Hyperkalemia and Familial Hyperkalemic Hypertension in 5100 Patients Referred to a Tertiary Hypertension Unit.
- Author
-
Tetti M, Burrello J, Hureaux M, Billon C, Clauser E, Veglio F, Rabbia F, Pasini B, Crisetti A, Jeunemaitre X, Mulatero P, and Monticone S
- Abstract
Background: Hyperkalemia is a frequent electrolyte alteration whose prevalence varies widely, depending on the adopted cutoff, the setting (inpatients versus outpatients), and the characteristics of the study population. Familial hyperkalemic hypertension (FHH) is a rare cause of hypertension, hyperkalemia, and hyperchloremic metabolic acidosis., Methods: In this retrospective observational study, we investigated the prevalence of hyperkalemia (serum K
+ >5.2 mmol/L on 2 repeated measurements) in 5100 referred patients affected by arterial hypertension, the potential causes, and the associated cardiovascular risk profile., Results: Overall, 374 (7.3%) patients had hyperkalemia. This was associated with drugs known to increase K+ levels (74.6%), chronic kidney disease (33.7%), or both (24.3%). Among the 60 patients with unexplained hyperkalemia, 3 displayed a clinical and biochemical phenotype suggestive of FHH that was genetically confirmed in 2 of them (0.04% in the entire cohort). FHH prevalence rose to 3.3% in patients with unexplained hyperkalemia and up to 29% (2/7) if they had serum K+ >5.8 mmol/L. The genetic cause of FHH was a missense variant affecting the acidic motif of WNK1 in 1 family and a rare CUL3 splicing variant, whose functional significance was confirmed by a minigene assay in another. Finally, we observed a significant association between hyperkalemia and the occurrence of cardiovascular events, metabolic syndrome, and organ damage, independent of potential confounding factors., Conclusions: The identification of hyperkalemia in patients with hypertensive has prognostic implications. A timely diagnosis of FHH is important for effective management of hypertension, electrolyte imbalance correction with tailored treatment, and genetic counseling.- Published
- 2024
- Full Text
- View/download PDF
39. Diagnostic Accuracy of Aldosterone and Renin Measurement by Chemiluminescence for Screening of Patients with Primary Aldosteronism.
- Author
-
Tetti M, Burrello J, Goi J, Parasiliti-Caprino M, Gioiello G, Settanni F, Monticone S, Mulatero P, and Mengozzi G
- Subjects
- Humans, Female, Middle Aged, Male, Adult, ROC Curve, Prospective Studies, Hypertension blood, Hypertension diagnosis, Aged, Reproducibility of Results, Hyperaldosteronism diagnosis, Hyperaldosteronism blood, Aldosterone blood, Renin blood, Luminescent Measurements methods
- Abstract
Primary aldosteronism (PA) is the most common cause of endocrine arterial hypertension, and the suggested screening test for case detection is the aldosterone-to-renin ratio (ARR) or aldosterone-to-direct renin ratio (ADRR) based on radio-immunoassay (RIA) and chemiluminescence assay (CLIA), respectively. The objective of our study was to evaluate the reliability of CLIA for aldosterone and renin measurement and the diagnostic performance of ADRR. A prospective cohort of 1110 patients referred to a single laboratory medicine center underwent measurement of aldosterone and direct renin concentration (DRC) by CLIA and measurement of aldosterone and plasma renin activity (PRA) by RIA. Of 1110 patients, 640 obtained a final diagnosis of hypertension, and 90 of these patients were diagnosed with PA. Overall, between-method correlation was highly significant for aldosterone concentrations ( R = 0.945, p < 0.001) and less strong but significant for DRC/PRA ( R = 0.422, p < 0.001). Among hypertensive patients, in PA cases, the areas under the receiver operator characteristics (ROC) curves were 0.928 (95% confidence interval 0.904-0.954) for ADRR and 0.943 (95% confidence interval 0.920-0.966) for ARR and were comparable and not significantly different. The highest accuracy was obtained with an ADRR cut-off of 25 (ng/L)/(mIU/L), displaying a sensitivity of 91% and a specificity of 85%. The chemiluminescence assay for aldosterone and DRC is a reliable method for PA diagnosis compared to the classical RIA method.
- Published
- 2024
- Full Text
- View/download PDF
40. Addressing Heterogeneity in Direct Analysis of Extracellular Vesicles and Their Analogs by Membrane Sensing Peptides as Pan-Vesicular Affinity Probes.
- Author
-
Gori A, Frigerio R, Gagni P, Burrello J, Panella S, Raimondi A, Bergamaschi G, Lodigiani G, Romano M, Zendrini A, Radeghieri A, Barile L, and Cretich M
- Subjects
- Humans, Extracellular Vesicles metabolism, Peptides metabolism, Biomarkers metabolism
- Abstract
Extracellular vesicles (EVs), crucial mediators of cell-to-cell communication, hold significant diagnostic potential due to their ability to concentrate protein biomarkers in bodily fluids. However, challenges in isolating EVs from biological specimens hinder their widespread use. The preferred strategy involves direct analysis, integrating isolation and analysis solutions, with immunoaffinity methods currently dominating. Yet, the heterogeneous nature of EVs poses challenges, as proposed markers may not be as universally present as thought, raising concerns about biomarker screening reliability. This issue extends to EV-mimics, where conventional methods may lack applicability. Addressing these challenges, the study reports on Membrane Sensing Peptides (MSP) as pan-vesicular affinity ligands for both EVs and their non-canonical analogs, streamlining capture and phenotyping through Single Molecule Array (SiMoA). MSP ligands enable direct analysis of circulating EVs, eliminating the need for prior isolation. Demonstrating clinical translation, MSP technology detects an EV-associated epitope signature in serum and plasma, distinguishing myocardial infarction from stable angina. Additionally, MSP allow analysis of tetraspanin-lacking Red Blood Cell-derived EVs, overcoming limitations associated with antibody-based methods. Overall, the work underlines the value of MSP as complementary tools to antibodies, advancing EV analysis for clinical diagnostics and beyond, and marking the first-ever peptide-based application in SiMoA technology., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)
- Published
- 2024
- Full Text
- View/download PDF
41. Transcriptomic signature of stress-induced premature senescence in cardiomyocytes.
- Author
-
Rendon-Angel A, Lazzarini E, Cascione L, Burrello J, Goshovska Y, Biemmi V, Panella S, Bolis S, Colucci M, Altomare C, Rinaldi A, Torre T, Alimonti A, and Barile L
- Subjects
- Animals, Gene Expression Profiling, Cells, Cultured, Humans, Stress, Physiological genetics, Gene Expression Regulation, Myocytes, Cardiac metabolism, Myocytes, Cardiac pathology, Transcriptome, Cellular Senescence genetics
- Published
- 2024
- Full Text
- View/download PDF
42. Unilateral Primary Aldosteronism: Long-Term Disease Recurrence After Adrenalectomy.
- Author
-
Tetti M, Brüdgam D, Burrello J, Udager AM, Riester A, Knösel T, Beuschlein F, Rainey WE, Reincke M, and Williams TA
- Subjects
- Humans, Adrenalectomy, Aldosterone, Neoplasm Recurrence, Local surgery, Retrospective Studies, Hyperaldosteronism diagnosis, Hyperaldosteronism genetics, Hyperaldosteronism surgery, Adrenocortical Adenoma genetics, Adrenocortical Adenoma surgery, Adenoma surgery
- Abstract
Background: Primary aldosteronism (PA) is frequently caused by a unilateral aldosterone-producing adenoma with a PA-driver mutation. Unilateral adrenalectomy has a high probability of short-term biochemical remission, but long-term postsurgical outcomes are relatively undefined. Our objective was to investigate the incidence of long-term recurrence of PA in individuals with postsurgical short-term biochemical remission., Methods: Adrenalectomized patients for unilateral PA were included from a single referral center. Histopathology and outcomes were assessed according to international histopathology of unilateral primary aldosteronism and PASO (Primary Aldosteronism Surgical Outcome) consensuses. Genotyping was performed using CYP11B2 (aldosterone synthase)-guided sequencing., Results: Classical adrenal histopathology, exemplified by a solitary aldosterone-producing adenoma, was observed in 78% of 90 adrenals, compared with 22% with nonclassical histopathology. The classical group displayed higher aldosterone-to-renin ratios ( P =0.013) and lower contralateral ratios ( P =0.008). Outcome assessments at both short (12 months [7; 12]) and long (89 months [48; 124]) terms were available for 57 patients. At short-term assessment, 53 (93%) displayed complete biochemical success (43 classical and 10 nonclassical), but long-term assessment demonstrated biochemical PA recurrence in 12 (23%) with an overrepresentation of the nonclassical histopathology (6 [60%] of 10 nonclassical histopathology versus 6 [14%] of 43 classical histopathology; P =0.005). PA-driver mutations were identified in 97% of 64 aldosterone-producing adenomas; there was no association of the aldosterone-producing adenoma genotype with PA recurrence., Conclusions: A substantial proportion of individuals display postsurgical biochemical recurrence of PA, which is related to the histopathology of the resected adrenal gland. These findings emphasize the role of histopathology and the requirement for continued outcome assessment in the management of surgically treated patients for PA., Competing Interests: None.
- Published
- 2024
- Full Text
- View/download PDF
43. Intracoronary delivery of extracellular vesicles from human cardiac progenitor cells reduces infarct size in porcine acute myocardial infarction.
- Author
-
Emmert MY, Burrello J, Wolint P, Hilbe M, Andriolo G, Balbi C, Provasi E, Turchetto L, Radrizzani M, Nazari-Shafti TZ, Cesarovic N, Neuber S, Falk V, Hoerstrup SP, Hemetsberger R, Gyöngyösi M, Barile L, and Vassalli G
- Subjects
- Humans, Swine, Animals, Coronary Vessels, Stem Cells, Myocardial Infarction therapy, Extracellular Vesicles
- Published
- 2024
- Full Text
- View/download PDF
44. Profile of matrix-entrapped extracellular vesicles of microenvironmental and infiltrating cell origin in decellularized colorectal cancer and adjacent mucosa.
- Author
-
Tassinari S, D'Angelo E, Caicci F, Grange C, Burrello J, Fassan M, Brossa A, Bao RQ, Spolverato G, Agostini M, Collino F, and Bussolati B
- Abstract
Cellular elements that infiltrate and surround tumours and pre-metastatic tissues have a prominent role in tumour invasion and growth. The extracellular vesicles specifically entrapped and stored within the extracellular matrix (ECM-EVs) may reflect the different populations of the tumour microenvironment and their change during tumour progression. However, their profile is at present unknown. To elucidate this aspect, we isolated and characterized EVs from decellularized surgical specimens of colorectal cancer and adjacent colon mucosa and analyzed their surface marker profile. ECM-EVs in tumours and surrounding mucosa mainly expressed markers of lymphocytes, natural killer cells, antigen-presenting cells, and platelets, as well as epithelial cells, representing a multicellular microenvironment. No difference in surface marker expression was observed between tumour and mucosa ECM-EVs in stage II-III tumours. At variance, in the colon mucosa adjacent to stage IV carcinomas, ECM-EV profile showed a significantly increased level of immune, epithelial and platelet markers in comparison to the matrix of the corresponding tumour. The increase of EVs from immune cells and platelets was not observed in the mucosa adjacent to low-stage tumours. In addition, CD25, a T-lymphocyte marker, resulted specifically overexpressed by ECM-EVs from stage IV carcinomas, possibly correlated with the pro-tolerogenic environment found in the corresponding tumour tissue. These results outline the tissue microenvironmental profile of EVs in colorectal carcinoma-derived ECM and unveil a profound change in the healthy mucosa adjacent to high-stage tumours., Competing Interests: The authors declare no conflicts of interest., (© 2024 The Authors. Journal of Extracellular Biology published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.)
- Published
- 2024
- Full Text
- View/download PDF
45. A dynamic clamping approach using in silico IK1 current for discrimination of chamber-specific hiPSC-derived cardiomyocytes.
- Author
-
Altomare C, Bartolucci C, Sala L, Balbi C, Burrello J, Pietrogiovanna N, Burrello A, Bolis S, Panella S, Arici M, Krause R, Rocchetti M, Severi S, and Barile L
- Subjects
- Humans, Myocytes, Cardiac, Constriction, Reproducibility of Results, Induced Pluripotent Stem Cells, Atrial Fibrillation
- Abstract
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CM) constitute a mixed population of ventricular-, atrial-, nodal-like cells, limiting the reliability for studying chamber-specific disease mechanisms. Previous studies characterised CM phenotype based on action potential (AP) morphology, but the classification criteria were still undefined. Our aim was to use in silico models to develop an automated approach for discriminating the electrophysiological differences between hiPSC-CM. We propose the dynamic clamp (DC) technique with the injection of a specific I
K1 current as a tool for deriving nine electrical biomarkers and blindly classifying differentiated CM. An unsupervised learning algorithm was applied to discriminate CM phenotypes and principal component analysis was used to visualise cell clustering. Pharmacological validation was performed by specific ion channel blocker and receptor agonist. The proposed approach improves the translational relevance of the hiPSC-CM model for studying mechanisms underlying inherited or acquired atrial arrhythmias in human CM, and for screening anti-arrhythmic agents., (© 2023. The Author(s).)- Published
- 2023
- Full Text
- View/download PDF
46. Identification of a serum and urine extracellular vesicle signature predicting renal outcome after kidney transplant.
- Author
-
Burrello J, Monticone S, Burrello A, Bolis S, Cristalli CP, Comai G, Corradetti V, Grange C, Orlando G, Bonafè M, La Manna G, Barile L, and Bussolati B
- Subjects
- Humans, Endothelial Cells, Kidney, Biomarkers urine, Glomerular Filtration Rate, Kidney Transplantation, Extracellular Vesicles, Body Fluids
- Abstract
Background: A long-standing effort is dedicated towards the identification of biomarkers allowing the prediction of graft outcome after kidney transplant. Extracellular vesicles (EVs) circulating in body fluids represent an attractive candidate, as their cargo mirrors the originating cell and its pathophysiological status. The aim of the study was to investigate EV surface antigens as potential predictors of renal outcome after kidney transplant., Methods: We characterized 37 surface antigens by flow cytometry, in serum and urine EVs from 58 patients who were evaluated before, and at 10-14 days, 3 months and 1 year after transplant, for a total of 426 analyzed samples. The outcome was defined according to estimated glomerular filtration rate (eGFR) at 1 year., Results: Endothelial cells and platelets markers (CD31, CD41b, CD42a and CD62P) in serum EVs were higher at baseline in patients with persistent kidney dysfunction at 1 year, and progressively decreased after kidney transplant. Conversely, mesenchymal progenitor cell marker (CD1c, CD105, CD133, SSEEA-4) in urine EVs progressively increased after transplant in patients displaying renal recovery at follow-up. These markers correlated with eGFR, creatinine and proteinuria, associated with patient outcome at univariate analysis and were able to predict patient outcome at receiver operating characteristics curves analysis. A specific EV molecular signature obtained by supervised learning correctly classified patients according to 1-year renal outcome., Conclusions: An EV-based signature, reflecting the cardiovascular profile of the recipient, and the repairing/regenerative features of the graft, could be introduced as a non-invasive tool for a tailored management of follow-up of patients undergoing kidney transplant., (© The Author(s) 2022. Published by Oxford University Press on behalf of the ERA.)
- Published
- 2023
- Full Text
- View/download PDF
47. Erratum: Inflammatory extracellular vesicles prompt heart dysfunction via TLR4-dependent NF-κB activation: Erratum.
- Author
-
Biemmi V, Milano G, Ciullo A, Cervio E, Burrello J, Dei Cas M, Paroni R, Tallone T, Moccetti T, Pedrazzini G, Longnus S, Vassalli G, and Barile L
- Abstract
[This corrects the article DOI: 10.7150/thno.39072.]., (© The author(s).)
- Published
- 2023
- Full Text
- View/download PDF
48. Adrenal surgery for bilateral primary aldosteronism: an international retrospective cohort study.
- Author
-
Williams TA, Gong S, Tsurutani Y, Tezuka Y, Thuzar M, Burrello J, Wu VC, Yamazaki Y, Mulatero P, Sasano H, Stowasser M, Nishikawa T, Satoh F, and Reincke M
- Subjects
- Humans, Retrospective Studies, Adrenal Glands surgery, Aldosterone, Adrenalectomy, Hyperaldosteronism surgery
- Abstract
Competing Interests: We declare no competing interests. TAW and SG contributed equally to this work. This study was funded by Deutsche Forschungsgemeinschaft and by Else Kröner-Fresenius Stiftung. Editorial note: The Lancet Group takes a neutral position with respect to territorial claims in published maps and institutional affiliations.
- Published
- 2022
- Full Text
- View/download PDF
49. Risk stratification of patients with SARS-CoV-2 by tissue factor expression in circulating extracellular vesicles.
- Author
-
Burrello J, Caporali E, Gauthier LG, Pianezzi E, Balbi C, Rigamonti E, Bolis S, Lazzarini E, Biemmi V, Burrello A, Frigerio R, Martinetti G, Fusi-Schmidhauser T, Vassalli G, Ferrari E, Moccetti T, Gori A, Cretich M, Melli G, Monticone S, and Barile L
- Subjects
- Biomarkers metabolism, Humans, Reproducibility of Results, Risk Assessment methods, SARS-CoV-2, Thromboplastin metabolism, COVID-19 diagnosis, Extracellular Vesicles metabolism
- Abstract
Inflammatory response following SARS-CoV-2 infection results in substantial increase of amounts of intravascular pro-coagulant extracellular vesicles (EVs) expressing tissue factor (CD142) on their surface. CD142-EV turned out to be useful as diagnostic biomarker in COVID-19 patients. Here we aimed at studying the prognostic capacity of CD142-EV in SARS-CoV-2 infection. Expression of CD142-EV was evaluated in 261 subjects admitted to hospital for pneumonia and with a positive molecular test for SARS-CoV-2. The study population consisted of a discovery cohort of selected patients (n = 60) and an independent validation cohort including unselected consecutive enrolled patients (n = 201). CD142-EV levels were correlated with post-hospitalization course of the disease and compared to the clinically available 4C Mortality Score as referral. CD142-EV showed a reliable performance to predict patient prognosis in the discovery cohort (AUC = 0.906) with an accuracy of 81.7%, that was confirmed in the validation cohort (AUC = 0.736). Kaplan-Meier curves highlighted a high discrimination power in unselected subjects with CD142-EV being able to stratify the majority of patients according to their prognosis. We obtained a comparable accuracy, being not inferior in terms of prediction of patients' prognosis and risk of mortality, with 4C Mortality Score. The expression of surface vesicular CD142 and its reliability as prognostic marker was technically validated using different immunocapture strategies and assays. The detection of CD142 on EV surface gains considerable interest as risk stratification tool to support clinical decision making in COVID-19., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
50. De novo DNA methylation induced by circulating extracellular vesicles from acute coronary syndrome patients.
- Author
-
Schiano C, Balbi C, Burrello J, Ruocco A, Infante T, Fiorito C, Panella S, Barile L, Mauro C, Vassalli G, and Napoli C
- Subjects
- DNA (Cytosine-5-)-Methyltransferases genetics, DNA Methylation, Epigenesis, Genetic, Humans, I-kappa B Kinase genetics, Leukocytes, Mononuclear metabolism, Acute Coronary Syndrome diagnosis, Acute Coronary Syndrome genetics, Extracellular Vesicles metabolism
- Abstract
Background and Aims: DNA methylation is associated with gene silencing, but its clinical role in cardiovascular diseases (CVDs) remains to be elucidated. We hypothesized that extracellular vesicles (EVs) may carry epigenetic changes, showing themselves as a potentially valuable non-invasive diagnostic liquid biopsy. We isolated and characterized circulating EVs of acute coronary syndrome (ACS) patients and assessed their role on DNA methylation in epigenetic modifications., Methods: EVs were recovered from plasma of 19 ACS patients and 50 healthy subjects (HS). Flow cytometry, qRT-PCR, and Western blot (WB) were performed to evaluate both intra-vesicular and intra-cellular signals. ShinyGO, PANTHER, and STRING tools were used to perform GO and PPI network analyses., Results: ACS-derived EVs showed increased levels of DNA methyltransferases (DNMTs) (p<0.001) and Ten-eleven translocation (TET) genes reduction. Specifically, de novo methylation transcripts, as DNMT3A and DNMT3B, were significantly increased in plasma ACS-EVs. DNA methylation analysis on PBMCs from healthy donors treated with HS- and ACS-derived EVs showed an important role of DNMTs carried by EVs. PPI network analysis evidenced that ACS-EVs induced changes in PBMC methylome. In the most enriched subnetwork, the hub gene SRC was connected to NOTCH1, FOXO3, CDC42, IKBKG, RXRA, DGKG, BAIAP2 genes that were showed to have many molecular effects on various cell types into onset of several CVDs. Modulation in gene expression after ACS-EVs treatment was confirmed for SRC, NOTCH1, FOXO3, RXRA, DGKG and BAIAP2 (p<0.05)., Conclusions: Our data showed an important role for ACS-derived EVs in gene expression modulation through de novo DNA methylation signals, and modulating signalling pathways in target cells., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.