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1. Local ablation disrupts immune evasion in pancreatic cancer

Author

Musiu, Chiara, Adamo, Annalisa, Caligola, Simone, Agostini, Antonio, Frusteri, Cristina, Lupo, Francesca, Boschi, Federico, Busato, Alice, Poffe, Ornella, Anselmi, Cristina, Vella, Antonio, Wang, Tian, Dusi, Silvia, Piro, Geny, Carbone, Carmine, Tortora, Giampaolo, Marzola, Pasquina, D'Onofrio, Mirko, Crinò, Stefano Francesco, Corbo, Vincenzo, Scarpa, Aldo, Salvia, Roberto, Malleo, Giuseppe, Lionetto, Gabriella, Sartoris, Silvia, Ugel, Stefano, Bassi, Claudio, Bronte, Vincenzo, Paiella, Salvatore, and De Sanctis, Francesco

Published
2025
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5. Gluteal femoral subcutaneous and dermal adipose tissue in female.

Author

Conti, Giamaica, Zingaretti, Nicola, Busato, Alice, Quintero Sierra, Lindsey, Amuso, Domenico, Scarano, Antonio, Iorio, Eugenio Luigi, Amore, Roberto, Ossanna, Riccardo, Negri, Alessandro, Conti, Anita, Veronese, Sheila, De Francesco, Francesco, Riccio, Michele, Parodi, Pier Camillo, and Sbarbati, Andrea

Subjects
ADIPOSE tissues, HIGH resolution imaging, BUTTOCKS, SWEAT glands, CELLULITE
Abstract

Background: During the sexual maturation, gluteal femoral adipose tissue is subjected to numerous modifications, not observable in other regions, in particular in women and less in men. Other authors described this region, but they used imaging techniques having lower resolution, than MRI proposed in this study. High resolution imaging techniques might provide important and more detailed information about the anatomy of gluteal femoral region. Methods: This study has been performed using 7 T‐magnetic resonance imaging and ultrastructural analysis in order to provide accurate description of the subcutaneous adipose tissue and dermis of gluteal femoral region. In this study specimens harvested from cadavers and form living patients have been analyzed. Results: The results showed the presence of three layers: superficial, middle, and deep, characterized by different organization of fat lobules. High resolution imaging showed the adipose papilla that originates from dermis and protrude in subcutaneous adipose tissue. Adipose papilla is characterized by a peculiar morphology with a basement, a neck and a head and these elements represent the functional subunits of adipose papilla. Moreover, ultrastructural study evidenced the relationship between adipocytes and sweat glands, regulated by lipid vesicles. Conclusions: This study provides important information about subcutaneous and dermal fat anatomy of gluteal femoral region, improving the past knowledge, and move toward a better understanding of the cellulite physiopathology. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Resting‐state functional magnetic resonance imaging reveals functional connectivity alteration in the experimental autoimmune encephalomyelitis model of multiple sclerosis.

Author

Bontempi, Pietro, Piccolantonio, Giusi, Busato, Alice, Conti, Anita, Angelini, Gabriele, Lopez, Nicola, Bani, Alessandro, Constantin, Gabriela, and Marzola, Pasquina

Subjects
FUNCTIONAL magnetic resonance imaging, FUNCTIONAL connectivity, MULTIPLE sclerosis, MAGNETIC resonance imaging, MYELIN oligodendrocyte glycoprotein
Abstract

Multiple sclerosis (MS) is an autoimmune degenerative disease targeting white matter in the central nervous system. The most common animal model that mimics MS is experimental autoimmune encephalomyelitis (EAE) and it plays a crucial role in pharmacological research, from the identification of a therapeutic target to the in vivo validation of efficacy. Magnetic resonance imaging (MRI) is largely used to detect MS lesions, and resting‐state functional MRI (rsfMRI) to investigate alterations in the brain functional connectivity (FC). MRI was mainly used in EAE studies to detect lesions in the spinal cord and brain. The current longitudinal MRI study aims to validate rsfMRI as a biomarker of the disease progression in the myelin oligodendrocyte glycoprotein 35–55 induced EAE animal model of MS. MR images were acquired 14, 25, and 50 days postimmunization. Seed‐based analysis was used to investigate the whole‐brain FC with some predefined areas, such as the thalamic regions, cerebellum, motor and somatosensory cortex. When compared with the control group, the EAE group exhibited a slightly altered FC and a decreasing trend in the total number of activated voxels along the disease progression. The most interesting result regards the whole‐brain FC with the cerebellum. A hyperconnectivity behavior was found at an early phase and a significant reduced connectivity at a late phase. Moreover, we found a negative correlation between the total number of activated voxels during the late phase and the cumulative disease index. The results obtained provide a clinically relevant experimental platform that may be pivotal for the elucidation of the key mechanisms of accumulation of irreversible disability, as well as the development of innovative therapies for MS. Moreover, the negative correlation between the disease severity and the size of the activated area suggests a possible research pathway to follow for the resolution of the clinico‐radiological paradox. [ABSTRACT FROM AUTHOR]

Published
2024
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7. In Vitro Study of a Novel Vibrio alginolyticus-Based Collagenase for Future Medical Application

Author

Quintero Sierra, Lindsey Alejandra, primary, Biswas, Reetuparna, additional, Busato, Alice, additional, Conti, Anita, additional, Ossanna, Riccardo, additional, Conti, Giamaica, additional, Zingaretti, Nicola, additional, Caputo, Michele, additional, Cuppari, Christian, additional, Parodi, Pier Camillo, additional, Sbarbati, Andrea, additional, Riccio, Michele, additional, and De Francesco, Francesco, additional

Published
2023
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8. Highly Pluripotent Adipose-Derived Stem Cell–Enriched Nanofat: A Novel Translational System in Stem Cell Therapy

Author

Quintero Sierra, Lindsey Alejandra, primary, Biswas, Reetuparna, additional, Conti, Anita, additional, Busato, Alice, additional, Ossanna, Riccardo, additional, Zingaretti, Nicola, additional, Parodi, Pier Camillo, additional, Conti, Giamaica, additional, Riccio, Michele, additional, Sbarbati, Andrea, additional, and De Francesco, Francesco, additional

Published
2023
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9. Doped Ferrite Nanoparticles Exhibiting Self-Regulating Temperature as Magnetic Fluid Hyperthermia Antitumoral Agents, with Diagnostic Capability in Magnetic Resonance Imaging and Magnetic Particle Imaging

Author

Vurro, Federica, primary, Gerosa, Marco, additional, Busato, Alice, additional, Muccilli, Matilde, additional, Milan, Emil, additional, Gaudet, Jeff, additional, Goodwill, Patrick, additional, Mansfield, James, additional, Forlin, Enrico, additional, Negri, Alessandro, additional, Gherlinzoni, Filippo, additional, Morana, Giovanni, additional, Gottardi, Michele, additional, Matteazzi, Paolo, additional, Wintermark, Max, additional, Speghini, Adolfo, additional, and Marzola, Pasquina, additional

Published
2022
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11. An iron-oxide nanoparticle with therapeutic capability in Magnetic Fluid Hyperthermia and diagnostic capability in MRI and MPI

Author

Vurro, Federica, Gerosa, Marco, Busato, Alice, Muccilli, Matilde, Milan, Emil, Gaudet, Jeff, Mansfield, James, Goodwill, Patrick, Forlin, Enrico, Gherlinzoni, Filippo, Morana, Gianni, Gottardi, Michele, Matteazzi, Paolo, Wintermark, Max, Speghini, Adolfo, and Marzola, Pasquina

Abstract

M55 belongs to a class of innovative nanomaterials, constituted by doped ferrite, with potential application in tumor therapy, as agents for Magnetic Fluid Hyperthermia (MFH), and in diagnosis, as contrast agents for MRI and MPI. Interestingly, such nanomaterials are characterized by a self-limiting temperature that can be modulated by adjusting the composition of the nanomaterial itself. M55 was coated with a double shell of citrate and glucose. We demonstrated that it has good capability as contrast agent for MRI and MPI. Moreover we tested its biocompatibility in a triple negative human breast cancer line and its efficacy as MFH agent in the same cell line. Despite relatively low SAR values in water solution, this agents was highly efficient in decreasing cell viability after two MFH treatments. Finally, we demonstrated that it can be useful to label cells for cell tracking in MPI. Although preliminary, these results are encouraging and push toward in vivo tests of such material., International Journal on Magnetic Particle Imaging, Vol 8 No 1 Suppl 1 (2022)

Published
2022
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12. Correction: De Francesco et al. In Vitro Characterization of Canine Microfragmented Adipose Tissue Non-Enzymatically Extracted from the Thigh and Lumbar Regions. Animals 2021, 11, 3231

Author

De Francesco, Francesco, primary, Riccio, Valentina, additional, Biswas, Reetuparna, additional, Busato, Alice, additional, Di Bella, Caterina, additional, Serri, Evelina, additional, Sbarbati, Andrea, additional, Zavan, Barbara, additional, Riccio, Michele, additional, and Palumbo Piccionello, Angela, additional

Published
2022
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13. Biocompatible, photo-responsive layer-by-layer polymer nanocapsules with an oil core: in vitro and in vivo study

Author

Di Cicco, Chiara, primary, Vecchione, Raffaele, additional, Quagliariello, Vincenzo, additional, Busato, Alice, additional, Tufano, Immacolata, additional, Bedini, Emiliano, additional, Gerosa, Marco, additional, Sbarbati, Andrea, additional, Boschi, Federico, additional, Marzola, Pasquina, additional, Maurea, Nicola, additional, and Netti, Paolo A., additional

Published
2022
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17. Nanoparticles exhibiting self-regulating temperature as innovative agents for Magnetic Fluid Hyperthermia

Author

Gerosa, Marco, primary, Grande, Marco Dal, additional, Busato, Alice, additional, Vurro, Federica, additional, Cisterna, Barbara, additional, Forlin, Enrico, additional, Gherlinzoni, Filippo, additional, Morana, Giovanni, additional, Gottardi, Michele, additional, Matteazzi, Paolo, additional, Speghini, Adolfo, additional, and Marzola, Pasquina, additional

Published
2021
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19. Biocompatible Iron–Boron Nanoparticles Designed for Neutron Capture Therapy Guided by Magnetic Resonance Imaging

Author

Torresan, Veronica, primary, Guadagnini, Andrea, additional, Badocco, Denis, additional, Pastore, Paolo, additional, Muñoz Medina, Guillermo Arturo, additional, Fernàndez van Raap, Marcela B., additional, Postuma, Ian, additional, Bortolussi, Silva, additional, Bekić, Marina, additional, Čolić, Miodrag, additional, Gerosa, Marco, additional, Busato, Alice, additional, Marzola, Pasquina, additional, and Amendola, Vincenzo, additional

Published
2020
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20. 4D Multimodal Nanomedicines Made of Nonequilibrium Au–Fe Alloy Nanoparticles

Author

Torresan, Veronica, primary, Forrer, Daniel, additional, Guadagnini, Andrea, additional, Badocco, Denis, additional, Pastore, Paolo, additional, Casarin, Maurizio, additional, Selloni, Annabella, additional, Coral, Diego, additional, Ceolin, Marcelo, additional, Fernández van Raap, Marcela B., additional, Busato, Alice, additional, Marzola, Pasquina, additional, Spinelli, Antonello E., additional, and Amendola, Vincenzo, additional

Published
2020
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22. Artificial dermal substitutes for tissue regeneration: comparison of the clinical outcomes and histological findings of two templates

Author

De Francesco, Francesco, primary, Busato, Alice, additional, Mannucci, Silvia, additional, Zingaretti, Nicola, additional, Cottone, Giuseppe, additional, Amendola, Francesco, additional, De Francesco, Marialuisa, additional, Merigo, Flavia, additional, Riccio, Valentina, additional, Vaienti, Luca, additional, Parodi, Pier Camillo, additional, Sbarbati, Andrea, additional, and Riccio, Michele, additional

Published
2020
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25. Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic

Author

Conti, Giamaica, primary, Zingaretti, Nicola, additional, Amuso, Domenico, additional, Dai Prè, Elena, additional, Brandi, Jessica, additional, Cecconi, Daniela, additional, Manfredi, Marcello, additional, Marengo, Emilio, additional, Boschi, Federico, additional, Riccio, Michele, additional, Amore, Roberto, additional, Iorio, Eugenio Luigi, additional, Busato, Alice, additional, De Francesco, Francesco, additional, Riccio, Valentina, additional, Parodi, Pier Camillo, additional, Vaienti, Luca, additional, and Sbarbati, Andrea, additional

Published
2020
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26. Hyperthermic nanocarriers for biomedical applications

Author

Vurro, Federica, Mannucci, Silvia, Davide, Prosperi, Perduca, Massimiliano, Concepción Jiménez López, Busato, Alice, Gerosa, Marco, Tambalo, Stefano, Marinozzi, Maria Rosaria, Boschi, Federico, Malatesta, Manuela, and Calderan, Laura

Subjects
nanoparticles, hyperthermia, hyperthermia, nanoparticles
Published
2019

29. Oil Core-PEG Shell Nanocarriers for In Vivo MRI Imaging

Author

Calcagno, Vincenzo, primary, Vecchione, Raffaele, additional, Quagliariello, Vincenzo, additional, Marzola, Pasquina, additional, Busato, Alice, additional, Giustetto, Pierangela, additional, Profeta, Martina, additional, Gargiulo, Sara, additional, Cicco, Chiara Di, additional, Yu, Hui, additional, Cassani, Marco, additional, Maurea, Nicola, additional, Mancini, Marcello, additional, Pellegrino, Teresa, additional, and Netti, Paolo A., additional

Published
2019
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30. MRI reveals therapeutical efficacy of stem cells: An experimental study on the SOD1(G93A) animal model

Author

Bontempi, Pietro, Busato, Alice, Bonafede, Roberta, Schiaffino, Lorenzo, Scambi, Ilaria, Sbarbati, Andrea, Mariotti, Raffaella, and Marzola, Pasquina

Subjects
Male, amyotrophic lateral sclerosis, MRI, SOD1(G93A), mesenchymal stem cells, voxel-based morphometry, Mice, Superoxide Dismutase-1, Image Processing, Computer-Assisted, Animals, Humans, Transgenes, Promoter Regions, Genetic, Behavior, Animal, Superoxide Dismutase, Stem Cells, Brain, Magnetic Resonance Imaging, Disease Models, Animal, Diffusion Tensor Imaging, Phenotype, Disease Progression, Anisotropy, Female, Biomarkers, Brain Stem, Stem Cell Transplantation
Abstract

The first part of the experiment identifies and validates MRI biomarkers distinctive of the disease progression in the transgenic superoxide dismutase gene (SOD1(G93A)) animal model. The second part assesses the efficacy of a mesenchymal stem cell-based therapy through the MRI biomarkers previously defined.The first part identifies MRI differences between SOD1(G93A) and healthy mice. The second part of the experiment follows the disease evolution of stem cell-treated and non-stem-cell treated SOD1(G93A) mice. The analysis focused on voxel-based morphometry and T2 mapping on the brain tissues, and T2-weighted imaging and diffusion tensor imaging (DTI) on the hind limbs.Comparing diseased mice to healthy control revealed gray matter alterations in the brainstem area, accompanied by increased T2 relaxation time. Differences in muscle volume, muscle signal intensity, fractional anisotropy, axial diffusivity, and radial diffusivity were measured in the hind limbs. In the comparison between stem cell-treated mice and nontreated ones, differences in muscle volume, muscle signal intensity, and DTI-derived maps were found.MRI-derived biomarkers can be used to identify differences between stem cell-treated and nontreated SOD1(G93A) mice. Magn Reson Med 79:459-469, 2018. © 2017 International Society for Magnetic Resonance in Medicine.

Published
2016

32. Characterization of magnetic nanoparticles from Magnetospirillum Gryphiswaldense as potential theranostics tools

Author

Orlando, T, Mannucci, Silvia, Fantechi, E, Conti, Giamaica, Tambalo, Stefano, Busato, Alice, Innocenti, C, Ghin, Leonardo, Bassi, Roberto, Arosio, P, Orsini, F, Sangregorio, C, Corti, M, Casula, M. F, Marzola, Pasquina, Lascialfari, A, and Sbarbati, Andrea

Subjects
Contrast Media, MRI, hyperthermia, contrast agent, magnetosomes, relaxometry, theranostics, hyperthermia, Theranostics, Magnetic Resonance Imaging, Xenograft Model Antitumor Assays, Theranostic Nanomedicine, Disease Models, Animal, Mice, Contrast agent, Neoplasms, Animals, Humans, Magnetosomes, Magnetospirillum, Magnetite Nanoparticles, Relaxometry, MRI
Abstract

We investigated the theranostic properties of magnetosomes (MNs) extracted from magnetotactic bacteria, promising for nanomedicine applications. Besides a physico-chemical characterization, their potentiality as mediators for magnetic fluid hyperthermia and contrast agents for magnetic resonance imaging, both in vitro and in vivo, are here singled out. The MNs, constituted by magnetite nanocrystals arranged in chains, show a superparamagnetic behaviour and a clear evidence of Verwey transition, as signature of magnetite presence. The phospholipid membrane provides a good protection against oxidation and the MNs oxidation state is stable over months. Using an alternate magnetic field, the specific absorption rate was measured, resulting among the highest reported in literature. The MRI contrast efficiency was evaluated by means of the acquisition of complete NMRD profiles. The transverse relaxivity resulted as high as the one of a former commercial contrast agent. The MNs were inoculated into an animal model of tumour and their presence was detected by magnetic resonance images two weeks after the injection in the tumour mass.

Published
2016
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35. Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Author

Busato,Alice, Bonafede,Roberta, Bontempi,Pietro, Scambi,Ilaria, Schiaffino,Lorenzo, Benati,Donatella, Malatesta,Manuela, Sbarbati,Andrea, Marzola,Pasquina, Mariotti,Raffaella, Busato,Alice, Bonafede,Roberta, Bontempi,Pietro, Scambi,Ilaria, Schiaffino,Lorenzo, Benati,Donatella, Malatesta,Manuela, Sbarbati,Andrea, Marzola,Pasquina, and Mariotti,Raffaella

Abstract

Alice Busato,1,* Roberta Bonafede,1,* Pietro Bontempi,2 Ilaria Scambi,1 Lorenzo Schiaffino,1 Donatella Benati,1 Manuela Malatesta,1 Andrea Sbarbati,1 Pasquina Marzola,3 Raffaella Mariotti1 1Department of Neurosciences, Biomedicine and Movement Sciences, School of Medicine, 2Department of Biotechnology, 3Department of Computer Science, University of Verona, Verona, Italy *These authors contributed equally to this work Purpose: Recent findings indicate that the beneficial effects of adipose stem cells (ASCs), reported in several neurodegenerative experimental models, could be due to their paracrine activity mediated by the release of exosomes. The aim of this study was the development and validation of an innovative exosome-labeling protocol that allows to visualize them with magnetic resonance imaging (MRI).Materials and methods: At first, ASCs were labeled using ultrasmall superparamagnetic iron oxide nanoparticles (USPIO, 4–6 nm), and optimal parameters to label ASCs in terms of cell viability, labeling efficiency, iron content, and magnetic resonance (MR) image contrast were investigated. Exosomes were then isolated from labeled ASCs using a standard isolation protocol. The efficiency of exosome labeling was assessed by acquiring MR images in vitro and in vivo as well as by determining their iron content. Transmission electron microscopy images and histological analysis were performed to validate the results obtained.Results: By using optimized experimental parameters for ASC labeling (200 µg Fe/mL of USPIO and 72 hours of incubation), it was possible to label 100% of the cells, while their viability remained comparable to unlabeled cells; the detection limit of MR images was of 102 and 2.5×103 ASCs in vitro and in vivo, respectively. Exosomes isolated from previously labeled ASCs retain nanoparticles, as demonstrated by transmission electron microscopy images. The detection limit by MRI was 3 &

Published
2016

36. Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Author

Marzola, Pasquina, primary, Busato, Alice, additional, Bonafede, Roberta, additional, Bontempi, Pietro, additional, Scambi, Ilaria, additional, Schiaffino, Lorenzo, additional, Benati, Donatella, additional, Malatesta, Manuela, additional, Sbarbati, Andrea, additional, and Mariotti, Raffaella, additional

Published
2016
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40. Biocompatible, photo-responsive layer-by-layer polymer nanocapsules with an oil core: in vitroand in vivostudy

Author

Di Cicco, Chiara, Vecchione, Raffaele, Quagliariello, Vincenzo, Busato, Alice, Tufano, Immacolata, Bedini, Emiliano, Gerosa, Marco, Sbarbati, Andrea, Boschi, Federico, Marzola, Pasquina, Maurea, Nicola, and Netti, Paolo A.

Abstract

In cancer therapy, stimulus-responsive drug delivery systems are of particular interest for reducing side effects in healthy tissues and improving drug selectivity in the tumoral ones. Here, a strategy for the preparation of a photo-responsive cross-linked trilayer deposited onto an oil-in-water nanoemulsion via a layer-by-layer technique is reported. The system is made of completely biocompatible materials such as soybean oil, egg lecithin and glycol chitosan, with heparin as the polymeric shell. The oil core is pre-loaded with curcumin as a model lipophilic active molecule with anti-tumoral properties. The trilayer cross-linkage is performed via a photoinitiator-free thiol-ene ‘click’ reaction. In particular, the system is implemented with an o-nitrobenzyl group functionalized with a thiol moiety which can perform both the thiol-ene ‘click’ reaction and the cleavage meant for controlled drug release at two different wavelengths, respectively. So the preparation and characterization of a photo-responsive natural nanocarrier (PNC) that is stable under physiological conditions owing to the thiol-ene cross-linkage are reported. PNC performance has been assessed in vitroon melanoma cells as well as in vivoon xenograft tumour-induced mice.

Published
2022
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41. Simple and Rapid Non-Enzymatic Procedure Allows the Isolation of Structurally Preserved Connective Tissue Micro-Fragments Enriched with SVF.

Author

Busato, Alice, De Francesco, Francesco, Biswas, Reetuparna, Mannucci, Silvia, Conti, Giamaica, Fracasso, Giulio, Conti, Anita, Riccio, Valentina, Riccio, Michele, and Sbarbati, Andrea

Subjects
*CONNECTIVE tissues, *MESENCHYMAL stem cells, *STEM cells, *PERICYTES, *ADIPOSE tissues, *CELL analysis
Abstract

The stromal vascular fraction (SVF) consists of a heterogeneous population of stem and stromal cells, generally obtained from adipose tissue by enzymatic digestion. For human cell-based therapies, mechanical process methods to obtain SVF represent an advantageous approach because they have fewer regulatory restrictions for their clinical use. The aim of this study was to characterize a novel commercial system for obtaining SVF from adipose tissue by a mechanical approach without substantial manipulations. Lipoaspirate samples collected from 27 informed patients were processed by a simple and fast mechanical system (by means of Hy-Tissue SVF). The Hy-Tissue SVF product contained a free cell fraction and micro-fragments of stromal connective tissue. The enzymatic digestion of the micro-fragments increased the yield of free cells (3.2 times) and CFU-F (2.4 times). Additionally, 10% of free cells from SVF were positive for CD34+, suggesting the presence of endothelial cells, pericytes, and potential adipose-derived stem cells (ADSC). Moreover, the SVF cells were able to proliferate and differentiate in vitro toward adipocytes, osteocytes, and chondrocytes. The immunophenotypic analysis of expanded cells showed positivity for typical mesenchymal stem cell markers. The Hy-Tissue SVF system allows the isolation of stromal vascular fraction, making this product of potential interest in regenerative medicine. [ABSTRACT FROM AUTHOR]

Published
2021
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42. Biocompatible, photo-responsive layer-by-layer polymer nanocapsules with an oil core: in vitro and in vivo study

Author

Chiara Di Cicco, Raffaele Vecchione, Vincenzo Quagliariello, Alice Busato, Immacolata Tufano, Emiliano Bedini, Marco Gerosa, Andrea Sbarbati, Federico Boschi, Pasquina Marzola, Nicola Maurea, Paolo A. Netti, Di Cicco, Chiara, Vecchione, Raffaele, Quagliariello, Vincenzo, Busato, Alice, Tufano, Immacolata, Bedini, Emiliano, Gerosa, Marco, Sbarbati, Andrea, Boschi, Federico, Marzola, Pasquina, Maurea, Nicola, and Netti, Paolo A

Subjects
nanoemulsions, Curcumin, photo-responsive nanocapsules, Polymers, Biomedical Engineering, Biophysics, nanoemulsion, Bioengineering, Biocompatible Materials, biodegradable polymer, Biochemistry, Biomaterials, Mice, Nanocapsules, Neoplasms, biodegradable polymers, drug delivery, Animals, Humans, Life Sciences–Engineering interface, Biotechnology
Abstract

In cancer therapy, stimulus-responsive drug delivery systems are of particular interest for reducing side effects in healthy tissues and improving drug selectivity in the tumoral ones. Here, a strategy for the preparation of a photo-responsive cross-linked trilayer deposited onto an oil-in-water nanoemulsion via a layer-by-layer technique is reported. The system is made of completely biocompatible materials such as soybean oil, egg lecithin and glycol chitosan, with heparin as the polymeric shell. The oil core is pre-loaded with curcumin as a model lipophilic active molecule with anti-tumoral properties. The trilayer cross-linkage is performed via a photoinitiator-free thiol-ene ‘click’ reaction. In particular, the system is implemented with an o -nitrobenzyl group functionalized with a thiol moiety which can perform both the thiol-ene ‘click’ reaction and the cleavage meant for controlled drug release at two different wavelengths, respectively. So the preparation and characterization of a photo-responsive natural nanocarrier (PNC) that is stable under physiological conditions owing to the thiol-ene cross-linkage are reported. PNC performance has been assessed in vitro on melanoma cells as well as in vivo on xenograft tumour-induced mice.

Published
2022

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