1. Molecular and fluorescence in situ hybridization analysis of a 10;11 rearrangement in a case of infant acute monocytic leukemia.
- Author
-
Roll P, Zattara-Cannoni H, Bustos-Bernard MC, Curtillet C, Michel G, and Vagner-Capodano AM
- Subjects
- Bone Marrow Transplantation, Chromosomes, Human, Pair 10 genetics, Chromosomes, Human, Pair 11 genetics, Histone-Lysine N-Methyltransferase, Humans, In Situ Hybridization, Fluorescence, Infant, Male, Myeloid-Lymphoid Leukemia Protein, Chromosome Inversion, Chromosomes, Human, Pair 10 ultrastructure, Chromosomes, Human, Pair 11 ultrastructure, DNA-Binding Proteins genetics, Leukemia, Monocytic, Acute genetics, Oncogene Proteins, Fusion genetics, Proto-Oncogenes, Transcription Factors genetics, Translocation, Genetic genetics
- Abstract
Fluorescence in situ hybridization (FISH) analysis in a case of infant acute monocytic leukemia M5 revealed a complex rearrangement between chromosomes 10 and 11, leading to the disruption of the MLL gene. Using two painting probes for chromosomes 10 and 11 and a specific probe for the MLL gene localized on 11q23, we observed a paracentric inversion of the 11q13-q23 fragment translocated to 10p12. Molecular analysis showed that AF10 localized on 10p12 was the fusion partner gene of MLL in this rearrangement (10;11). This report underlined the usefulness of FISH and molecular techniques in identifying complex rearrangements.
- Published
- 2002
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