45 results on '"Butian Zhang"'
Search Results
2. Retraction notice to 'LINC01116 targets miR-520a-3p and affects IL6R to promote the proliferation and migration of osteosarcoma cells through the Jak-stat signaling pathway' [Biomed. Pharmacother. 107 (2018) 270–282]
- Author
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Butian Zhang, Lili Yu, Ning Han, Zhenzhen Hu, Shuang Wang, Lei Ding, and Jinlan Jiang
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Published
- 2024
- Full Text
- View/download PDF
3. Carbon dot-based nanomaterials: a promising future nano-platform for targeting tumor-associated macrophages
- Author
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Yingying Miao, Shuang Wang, Butian Zhang, and Lin Liu
- Subjects
TAMs ,carbon dots (CDs) ,bioimaging ,theranostics ,macrophage modulation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The tumor microenvironment (TME) is the internal environment that tumors depend on for survival and development. Tumor-associated macrophages (TAMs), as an important part of the tumor microenvironment, which plays a crucial role in the occurrence, development, invasion and metastasis of various malignant tumors and has immunosuppressant ability. With the development of immunotherapy, eradicating cancer cells by activating the innate immune system has yielded encouraging results, however only a minority of patients show a lasting response. Therefore, in vivo imaging of dynamic TAMs is crucial in patient-tailored immunotherapy to identify patients who will benefit from immunotherapy, monitor efficacy after treatment, and identify alternative strategies for non-responders. Meanwhile, developing nanomedicines based on TAMs-related antitumor mechanisms to effectively inhibit tumor growth is expected to become a promising research field. Carbon dots (CDs), as an emerging member of the carbon material family, exhibit unexpected superiority in fluorescence imaging/sensing, such as near infrared imaging, photostability, biocompatibility and low toxicity. Their characteristics naturally integrate therapy and diagnosis, and when CDs are combined with targeted chemical/genetic/photodynamic/photothermal therapeutic moieties, they are good candidates for targeting TAMs. We concentrate our discussion on the current learn of TAMs and describe recent examples of macrophage modulation based on carbon dot-associated nanoparticles, emphasizing the advantages of their multifunctional platform and their potential for TAMs theranostics.
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- 2023
- Full Text
- View/download PDF
4. LncRNA MEG3 suppressed the progression of ovarian cancer via sponging miR-30e-3p and regulating LAMA4 expression
- Author
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Yang Liu, Yangchun Xu, Lei Ding, Lili Yu, Butian Zhang, and Dan Wei
- Subjects
LncRNA MEG3 ,LAMA4 ,miR-30e-3p ,Ovarian cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Background Ovarian cancer (OC) is a common female reproductive malignancy with a high mortality rate. Although LAMA4 was observed to be downregulated in OC cells, its mechanism in regulating OC metastasis is still unknown. This study aimed to investigate the effect of LAMA4 and its mechanism on OC. Methods To achieve this aim, a microarray analysis was performed to screen out the key genes involved in OC pathogenesis. Western-blot and qRT-PCR assays were also carried out to detect protein and mRNA expressions, respectively. A luciferase reporter assay was further used to confirm the direct interaction of miR-30e-3p with MEG3, and the direct interaction of miR-30e-3p with LAMA4 mRNA. Cytological experiments (CCK8, colony formation assay, wound-healing assay etc.) were then performed to explore the roles of miR-30e-3p, MEG3, and LAMA4 in OC cells. Results After carrying out microarray analysis, LAMA4 was confirmed as a key gene associated with OC pathogenesis. Research results proved that miR-30e-3p was markedly upregulated, while MEG3 and LAMA4 were noticeably downregulated in OC tissues and cells. The overexpression of LAMA4 significantly impaired the proliferation, migration, and invasion of OC cells. However, the upregulation of MEG3 increased the expression of LAMA4 by sponging miR-30e-3p, which alleviated the malignancy of OC cells. Conclusions Observations showed that forced LAMA4 overexpression could inhibit OC progression, which was regulated by MEG3 via sponging miR-30e-3p. The findings of this research could provide new insights into the mechanism by which MEG3 and LAMA4 exert their anti-oncogenic roles in OC progression. Trial registration Not applicable
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- 2020
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5. Airline Point-of-Care System on Seat Belt for Hybrid Physiological Signal Monitoring
- Author
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Xiaoqiang Ji, Zhi Rao, Wei Zhang, Chang Liu, Zimo Wang, Shuo Zhang, Butian Zhang, Menglei Hu, Peyman Servati, and Xiao Xiao
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point-of-care system ,wearable biosensors ,machine-learning ,airline industry security ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
With a focus on disease prevention and health promotion, a reactive and disease-centric healthcare system is revolutionized to a point-of-care model by the application of wearable devices. The convenience and low cost made it possible for long-term monitoring of health problems in long-distance traveling such as flights. While most of the existing health monitoring systems on aircrafts are limited for pilots, point-of-care systems provide choices for passengers to enjoy healthcare at the same level. Here in this paper, an airline point-of-care system containing hybrid electrocardiogram (ECG), breathing, and motion signals detection is proposed. At the same time, we propose the diagnosis of sleep apnea-hypopnea syndrome (SAHS) on flights as an application of this system to satisfy the inevitable demands for sleeping on long-haul flights. The hardware design includes ECG electrodes, flexible piezoelectric belts, and a control box, which enables the system to detect the original data of ECG, breathing, and motion signals. By processing these data with interval extraction-based feature selection method, the signals would be characterized and then provided for the long short-term memory recurrent neural network (LSTM-RNN) to classify the SAHS. Compared with other machine learning methods, our model shows high accuracy up to 84–85% with the lowest overfit problem, which proves its potential application in other related fields.
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- 2022
- Full Text
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6. LINC01116 Promotes Doxorubicin Resistance in Osteosarcoma by Epigenetically Silencing miR-424-5p and Inducing Epithelial-Mesenchymal Transition
- Author
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Ran Li, Qing Ruan, Jia Zheng, Butian Zhang, and Hongliang Yang
- Subjects
LINC01116 ,miR-424-5p ,HMGA2 ,drug resistance ,osteosarcoma ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Development of resistance to doxorubicin-based chemotherapy limits its curative effect in osteosarcoma. In the current study, we focused on investigating the mechanisms underlying the development of doxorubicin resistance in osteosarcoma.Methods: The human osteosarcoma cell line MG-63 and doxorubicin-resistant MG-63/Dox cells were used in this study. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of the long non-coding RNA LINC01116 in the two cell lines. Then, the specific shRNA for LINC01116 was employed to suppress LINC01116 expression in MG-63/Dox cells. Cell viability was assessed by the CCK-8 and colony formation assays. Cell migration and invasion were evaluated by the transwell assay. Moreover, the epithelial-mesenchymal transition (EMT)-related proteins, E-cadherin, vimentin, and N-cadherin were evaluated by Western blotting. The regulation of LINC01116 on miR-424-5p expression was examined using methylation-specific PCR, RNA immunoprecipitation, and Western blotting assay. The potential targeting of HMGA2 by miR-424-5p was predicted using the bioinformatics databases TargetScan and miRanda and verified by a dual-luciferase reporter assay.Results: LINC01116 was more highly expressed in MG-63/Dox cells than in MG-63 cells. Inhibition of LINC01116 suppressed cell viability, migration, and invasion, along with upregulating the expression of E-cadherin, downregulating vimentin, and attenuating doxorubicin resistance in MG-63/Dox cells. Further mechanism-related investigations indicated that LINC01116 regulated HMGA2 expression via the EZH2-associated silencing of miR-424-5p.Conclusion: LINC01116 exerts regulatory effects on doxorubicin resistance through the miR-424-5p axis, providing a potential approach to overcoming chemoresistance in osteosarcoma.
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- 2021
- Full Text
- View/download PDF
7. Corrigendum to 'LINC01116 targets miR-520a-3p and affects IL6R to promote the proliferation and migration of osteosarcoma cells through the Jak-stat signaling pathway' [Biomed. Pharmacother. 107 (2018) 270–282]
- Author
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Butian Zhang, Lili Yu, Ning Han, Zhenzhen Hu, Shuang Wang, Lei Ding, and Jinlan Jiang
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Published
- 2021
- Full Text
- View/download PDF
8. Corrigendum to 'LINC01116 targets miR-520a-3p and affects IL6R to promote the proliferation and migration of osteosarcoma cells through the Jak-stat signaling pathway' [Biomed. Pharmacother. 107 (2018) 270–282]
- Author
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Butian Zhang, Lili Yu, Ning Han, Zhenzhen Hu, Shuang Wang, Lei Ding, and Jinlan Jiang
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Published
- 2019
- Full Text
- View/download PDF
9. Translating History of Science Books into Chinese : Why? Which Ones? How?
- Author
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Butian, Zhang
- Published
- 2018
10. Supercooled water induced hysteretic transition in H2SO4-treated PEDOT:PSS
- Author
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Xinxin Song, Yanting Liu, Youwei Zhang, Butian Zhang, and Shun Wang
- Subjects
Materials Chemistry ,General Chemistry - Abstract
A temperature hysteretic transition, induced by supercooled water, is observed in both the electrical conductivity and Raman spectra signals of H2SO4-treated PEDOT:PSS.
- Published
- 2023
11. MiRNA-520a-3p Combined with Folic Acid Conjugated Fe2O3@PDA Multifunctional Nanoagents for MR Imagine and Antitumor Gene-Photothermal Therapy
- Author
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Xue Li, Shuang Wang, Qinzhe Gao, Na Li, Shanshan Dong, Yuwei Gao, Zuobin Wang, Butian Zhang, and Xiuxia He
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Bioengineering ,General Chemistry ,Electrical and Electronic Engineering - Abstract
Osteosarcoma (OS) is a primary malignant bone tumor that occurs mainly in adolescents. Researchers are devoting to develop combination therapy methods in a multifunctional nanoplatform for the treatment of osteosarcoma. The results of previous research have shown that up-regulation of miR- 520a-3p could induce anticancer effects in osteosarcoma. In order to improve the effect of gene therapy (GT), we attempted to carry miR-520a-3p in a multifunctional vector for comprehensive therapy. Fe 2 O 3 is a type of magnetic resonance imaging contrast that is widely used as a drug delivery agent. When coated with polydopamine (PDA), it can also be used as a photothermal therapy (PTT) agent (Fe 2 O 3 @ PDA). To deliver nanoagents targeted to a tumor site, folic acid (FA) conjugated with Fe 2 O 3 @PDA was manufactured as FA-Fe 2 O 3 @PDA. FA was chosen as the target molecule to enhance utilization and reduce toxicity of nanoparticles. However, the therapeutic efficacy of FA-Fe 2 O 3 -PDA combined with miR-520a-3p has not yet been studied. In this study, we synthesized FA-Fe 2 O 3 @PDA-miRNA and investigated the potential of combining PDA regulated PTT and miR-520a-3p regulated GT to kill osteosarcoma cells. The results indicated that down-regulation of interleukin 6 receptor (IL6R) by miR- 520a-3p and the photothermal ability of PDA could induce satisfactory anticancer effects in osteosarcoma, and the curative ratio was better than that used alone PTT or GT. Moreover, as a kind of T 2 magnetic contrast, miRNA-Fe 2 O 3 @PDA-FA can be used for magnetic resonance imaging (MRI). These findings indicated that miRNA-Fe 2 O 3 @PDA-FA is an effective anti-tumor nanovector for PTT combined with GT.
- Published
- 2023
12. Controllable Switching between Highly Rectifying Schottky and p–n Junctions in an Ionic MoS 2 Device
- Author
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Ruiheng Chang, Qiao Chen, Wang Shen, Youwei Zhang, Butian Zhang, and Shun Wang
- Subjects
Biomaterials ,Electrochemistry ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2023
13. Tunable Performance of Quantum Dot-MoS2 Hybrid Photodetectors via Interface Engineering
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Kexin Wang, Butian Zhang, Tianzi Ma, Xue-Wen Chen, Youwei Zhang, Shun Wang, Ruiheng Chang, and Jianwei Tang
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Responsivity ,Electron mobility ,Materials science ,business.industry ,Quantum dot ,Photodetector ,Optoelectronics ,General Materials Science ,Heterojunction ,Photodetection ,Electronic band structure ,business ,Absorption (electromagnetic radiation) - Abstract
Heterostructures of quantum dots (QDs) and two-dimensional (2D) materials show promising potential for photodetection applications owing to their combination of high optical absorption and good in-plane carrier mobility. In this work, the performance of QD-2D photodetectors is tuned by band engineering. Devices are fabricated by coating MoS2 nanosheets with InP QDs, type-I core-shell InP/ZnS QDs, and type-II core-shell InP/CdS QDs. Comparative spectroscopic and photoelectric studies of different hybrids show that the energy band alignment and shell thickness can influence the efficiency of charge transfer (CT), energy transfer (ET), and defect-related processes between QDs and MoS2. Benefiting from efficient CT between the QDs and MoS2, a significant enhancement of responsivity and detectivity is observed in thick-shell InP/CdS QD-MoS2 devices. Our results demonstrate the feasibility of using core-shell QDs for regulating the ET and CT efficiency in heterostructures and highlight the importance of interface band design in QD-2D and other low-dimensional photodetectors.
- Published
- 2021
14. Tailoring the Fluorescent and Electronic Properties of 2H-MoS2 by Step-by-Step Functionalization
- Author
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Changjiang Nie, Xin Yi, Shun Wang, Mingming Yin, Chuanwen Zhao, Ye Zhao, Liang Luo, Butian Zhang, Xinxin Song, and Youwei Zhang
- Subjects
General Energy ,Materials science ,Surface modification ,Nanotechnology ,Physical and Theoretical Chemistry ,Fluorescence ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Electronic properties - Abstract
Two-dimensional layered MoS2 has attracted tremendous attention because of its unique physical and chemical properties and promising application prospects. To further expand its applications to are...
- Published
- 2021
15. Tunable resonance Raman scattering of quantum dots in a nonlinear excitation regime
- Author
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Butian Zhang, Kexin Wang, Xinxin Song, Youwei Zhang, Xue-Wen Chen, and Shun Wang
- Subjects
General Physics and Astronomy ,Physical and Theoretical Chemistry - Abstract
Controllable tuning of electron–phonon coupling strength and excited state dynamics is important for the understanding of resonance Raman scattering in low-dimensional semiconductors. Here, we report a significant and reversible field-induced modulation in absolute resonance Raman intensity of quantum dots using ionic liquid gating. Meanwhile, a potential-dependent nonlinear relationship is present between Raman intensity and excitation power density. By exploring the parameter space within a time domain model, we find that the Raman intensity variation is mainly determined by the homogeneous linewidth. We further propose that the Fermi level positions and exciton species play key roles in the excited state decay rates.
- Published
- 2023
16. Thickness-Dependent Enhancement of Electronic Mobility of MoS2 Transistors via Surface Functionalization
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Changjiang Nie, Yuting Gao, Mingming Yin, Youwei Zhang, Xin Yi, Chuanwen Zhao, Shun Wang, Liang Luo, and Butian Zhang
- Subjects
Thickness dependent ,Materials science ,business.industry ,Transistor ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,law.invention ,General Energy ,chemistry ,Molybdenum ,law ,Surface modification ,Optoelectronics ,Physical and Theoretical Chemistry ,0210 nano-technology ,business - Abstract
The thickness dependence of the chemical and physical properties is one fundamental characteristic shared by many two-dimensional layered transition-metal dichalcogenides, including molybdenum disu...
- Published
- 2020
17. Facile synthesis of a porous polynorbornene with an azobenzene subunit: selective adsorption of 4-nitrophenol over 4-aminophenol in water
- Author
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Mu-Hua Huang, Shuifeng Wang, Wenhao Fan, Zhi-Hao Zhang, Yan Liu, Shi-Lu Chen, Butian Zhang, and Shan-Qing Peng
- Subjects
Polymers and Plastics ,Chemistry ,Organic Chemistry ,Diol ,Bioengineering ,ROMP ,Biochemistry ,chemistry.chemical_compound ,Adsorption ,Polymerization ,Azobenzene ,Selective adsorption ,Polymer chemistry ,Selectivity ,Norbornene - Abstract
This paper reports the design and facile synthesis of porous polynorbornenes containing a 4,4′-dialkoxy-azobenzene subunit (named Azo-POP-13 and Azo-POP-14). They were synthesized by the robust reductive azo-coupling polymerization of the Ring-Opening-Metathesis-Polymerization (ROMP) product on bis-para-nitrophenylether of the norbornene diol. The polymerization rate of exo-NBNPE (kapp = 18.7 × 10−2 s−1) is faster than that of endo-NBNPE (kapp = 3.64 × 10−2 s−1). The as-synthesized Azo-POP-13 was found to selectively adsorb 4-nitrophenol (4-NP) over 4-aminophenol (4-AP) in water, with a selectivity up to 14.6 and good recyclability.
- Published
- 2020
18. Microfluidic chip enabled one-step synthesis of biofunctionalized CuInS2/ZnS quantum dots
- Author
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Ken-Tye Yong, Yuguo Tang, Shuwen Zeng, Hanbin Ma, Liwei Liu, Butian Zhang, and Siyi Hu
- Subjects
Materials science ,biology ,Microfluidics ,Biomedical Engineering ,Bioengineering ,One-Step ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Biochemistry ,Fluorescence ,0104 chemical sciences ,Microfluidic chip ,Nanocrystal ,Folic acid ,Quantum dot ,biology.protein ,Bovine serum albumin ,0210 nano-technology - Abstract
Biofunctionalized quantum dots (QDs) are effective target fluorescent labels for bioimaging. However, conventional synthesis of biofunctionalized I-III-VI core-shell CuInS2/ZnS QDs requires complex bench-top operations, resulting in limited product performance and variety, and is not amenable to a 'one-step' approach. In this work, we have successfully demonstrated a fully automated method for preparing denatured bovine serum albumin (dBSA)-CuInS2/ZnS QDs by introducing microfluidic (MF) chips to synthesize biofunctionalized QDs, hence establishing a 'one-step' procedure. We have also studied and optimized the reaction synthesis parameters. The emission wavelength of the dBSA-CuInS2/ZnS QDs is located in the near-infrared range and can be tuned from 650 to 750 nm by simply varying the reaction parameters. In addition, the 'one-step'-synthesized dBSA-CuInS2/ZnS QDs have a long average fluorescence lifetime of 153.76 ns and a small particle size of 5 ± 2 nm. To demonstrate the applicability of the 'one-step'-synthesized dBSA-CuInS2/ZnS QDs in bioimaging studies, we modified the QDs with folic acid and hyaluronic acid, and then performed target bioimaging and cytotoxicity tests on macrophages, liver cancer cells and pancreatic cancer cells. The cell images show that the red emission signals originate from the QDs, which indicates that the dBSA-CuInS2/ZnS QDs prepared by the MF approach are suitable optical contrast agents for target bioimaging. This 'one-step' MF-based QD synthesis approach could serve as a rapid, cost-effective, and small-scale nanocrystal production platform for complex QD formulations for a wide range of bioapplications.
- Published
- 2020
19. Tunable Performance of Quantum Dot-MoS
- Author
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Ruiheng, Chang, Kexin, Wang, Youwei, Zhang, Tianzi, Ma, Jianwei, Tang, Xue-Wen, Chen, Butian, Zhang, and Shun, Wang
- Abstract
Heterostructures of quantum dots (QDs) and two-dimensional (2D) materials show promising potential for photodetection applications owing to their combination of high optical absorption and good in-plane carrier mobility. In this work, the performance of QD-2D photodetectors is tuned by band engineering. Devices are fabricated by coating MoS
- Published
- 2021
20. Study of Charge Transfer at the Quantum Dot–Graphene Interface by Raman Spectroscopy
- Author
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Kexin Wang, Ruiheng Chang, Youwei Zhang, Xin Yi, Butian Zhang, and Shun Wang
- Subjects
Materials science ,business.industry ,Graphene ,Interface (computing) ,Charge (physics) ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,law.invention ,symbols.namesake ,General Energy ,Quantum dot ,law ,symbols ,Optoelectronics ,Physical and Theoretical Chemistry ,0210 nano-technology ,business ,Raman spectroscopy ,Quantum - Abstract
Charge transfer at the interface is important for the optoelectronic and photochemical applications of quantum dot-two-dimensional material (QD-2D) hybrids. In this work, Raman spectroscopy was exp...
- Published
- 2019
21. Response Monitoring to Neoadjuvant Chemotherapy in Osteosarcoma Using Dynamic Contrast-Enhanced MR Imaging
- Author
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Yi-meng Dai, Kai-ming Xue, Jin-lan Jiang, Lin Liu, Yan-Ni Zeng, Butian Zhang, and Qian Zheng
- Subjects
Chemotherapy ,Receiver operating characteristic ,business.industry ,medicine.medical_treatment ,Diagnostic accuracy ,medicine.disease ,Mr imaging ,Primary tumor ,Dynamic contrast ,Mann–Whitney U test ,medicine ,Osteosarcoma ,Nuclear medicine ,business - Abstract
To evaluate the dynamic contrast-enhanced MRI parameters for monitoring the neoadjuvant chemotherapy (NAC) response in osteosarcoma prospectively. A total of 19 patients with osteosarcoma were recruited and underwent two dynamic contrast-enhanced MRI (DCE-MRI) examinations before and after chemotherapy. Patients with ≥ 90% tumor necrosis were defined as responders (10 patients), and those
- Published
- 2019
22. LINC01116 targets miR-520a-3p and affects IL6R to promote the proliferation and migration of osteosarcoma cells through the Jak-stat signaling pathway
- Author
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Butian Zhang, Lili Yu, Zhenzhen Hu, Shuang Wang, Ning Han, Lei Ding, and Jin-lan Jiang
- Subjects
0301 basic medicine ,Cell Survival ,Down-Regulation ,Mice, Nude ,Biology ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,Cell Movement ,Cell Line, Tumor ,microRNA ,medicine ,Animals ,Humans ,MTT assay ,RNA, Messenger ,Viability assay ,3' Untranslated Regions ,Cell Proliferation ,Janus Kinases ,Pharmacology ,Mice, Inbred BALB C ,Osteosarcoma ,Base Sequence ,medicine.diagnostic_test ,Microarray analysis techniques ,JAK-STAT signaling pathway ,General Medicine ,Receptors, Interleukin-6 ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,STAT Transcription Factors ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,Female ,RNA, Long Noncoding ,Signal transduction ,Signal Transduction - Abstract
Objective The purpose of this study was to find out the important lncRNA-miRNA-mRNA axis and pathway in osteosarcoma (OS) through bioinformatics analysis and verify the biological functions of lncRNA/miRNA/mRNA in OS through in vitro and in vivo assays. Methods The differential expression mRNAs and lncRNAs were identified through microarray analysis, and the altered pathways were identified by GSEA. The Pearson Coefficient was used to analyze the correlations between mRNAs and lncRNAs. Kaplan-Meier survival analysis was preformed using patient information in GEO database. Their target miRNAs were predicted by Targetscan and miRanda database and confirmed by dual luciferase reporter assay. QRT-PCR were utilized to detected the relative expressions of mRNAs, miRNAs and lncRNAs. The expressions of IL6R protein and pathway related proteins were detected by western blot. OS cell viability, migration and apoptosis were determined through MTT assay, Transwell assay and flow cytometry. Tumor formation in nude mice verified the influence of LINC01116 in vivo. Results The Jak-stat signaling pathway was activated in OS tissues. LINC01116 expression was positively correlated with IL6R expression. MiR-520a-3p targeted the 3’-UTR of LINC01116 and IL6R. Lower expression levels of miR-520a-3p significantly correlated with shorter survival of patients. LINC01116 and IL6R were up-regulated while miR-520a-3p was down-regulated in OS. LINC01116 and IL6R promoted the viability and migration of OS cells, while miR-520a-3p acted as a tumor suppressor. MiR-520a-3p inhibitor could rescue the suppressive effects of si-LINC011116 and si-IL6R on OS development. The Jak-stat signaling pathway related proteins were also down-regulated by miR-520a-3p. Down-regulation of LINC01116 inhibited the tumor growth in nude mice. Conclusion LINC01116 up-regulated IL6R in OS through targeting miR-520a-3p, thus activating the Jak-stat signaling pathway and promoting the progression of OS.
- Published
- 2018
23. LINC01116 Promotes Doxorubicin Resistance in Osteosarcoma by Epigenetically Silencing miR-424-5p and Inducing Epithelial-Mesenchymal Transition
- Author
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Butian Zhang, Ran Li, Hongliang Yang, Jia Zheng, and Qing Ruan
- Subjects
Pharmacology ,HMGA2 ,drug resistance ,biology ,Chemistry ,lcsh:RM1-950 ,Cell migration ,Vimentin ,LINC01116 ,medicine.disease ,miR-424-5p ,Small hairpin RNA ,lcsh:Therapeutics. Pharmacology ,Cell culture ,osteosarcoma ,biology.protein ,Cancer research ,medicine ,Gene silencing ,Osteosarcoma ,Pharmacology (medical) ,Epithelial–mesenchymal transition ,Viability assay ,Original Research - Abstract
Background: Development of resistance to doxorubicin-based chemotherapy limits its curative effect in osteosarcoma. In the current study, we focused on investigating the mechanisms underlying the development of doxorubicin resistance in osteosarcoma.Methods: The human osteosarcoma cell line MG-63 and doxorubicin-resistant MG-63/Dox cells were used in this study. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of the long non-coding RNA LINC01116 in the two cell lines. Then, the specific shRNA for LINC01116 was employed to suppress LINC01116 expression in MG-63/Dox cells. Cell viability was assessed by the CCK-8 and colony formation assays. Cell migration and invasion were evaluated by the transwell assay. Moreover, the epithelial-mesenchymal transition (EMT)-related proteins, E-cadherin, vimentin, and N-cadherin were evaluated by Western blotting. The regulation of LINC01116 on miR-424-5p expression was examined using methylation-specific PCR, RNA immunoprecipitation, and Western blotting assay. The potential targeting of HMGA2 by miR-424-5p was predicted using the bioinformatics databases TargetScan and miRanda and verified by a dual-luciferase reporter assay.Results: LINC01116 was more highly expressed in MG-63/Dox cells than in MG-63 cells. Inhibition of LINC01116 suppressed cell viability, migration, and invasion, along with upregulating the expression of E-cadherin, downregulating vimentin, and attenuating doxorubicin resistance in MG-63/Dox cells. Further mechanism-related investigations indicated that LINC01116 regulated HMGA2 expression via the EZH2-associated silencing of miR-424-5p.Conclusion: LINC01116 exerts regulatory effects on doxorubicin resistance through the miR-424-5p axis, providing a potential approach to overcoming chemoresistance in osteosarcoma.
- Published
- 2021
24. Synthesis and surface modification of quantum dots for biophotonics applications
- Author
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Butian Zhang, Yong Ken Tye, School of Electrical and Electronic Engineering, and Centre for OptoElectronics and Biophotonics
- Subjects
Biophotonics ,Materials science ,business.industry ,Quantum dot ,Engineering::Electrical and electronic engineering [DRNTU] ,Optoelectronics ,Surface modification ,Nanotechnology ,business - Abstract
In the last two decades, quantum dots (QDs) have demonstrated great potential for biophotonics applications such as optical imaging and analytes sensing. For these purposes, cadmium-based (Cd-based) QDs are most commonly used because of their excellent optical properties, while cadmium-free (Cd-free) QDs are attracting increasing attention due to the low toxicity of their components. Generally, for use in biological fluids, QDs are either prepared using the aqueous synthesis method or the hot colloidal synthesis method followed by a phase transfer process. This thesis studies the preparation of water-dispersible Cd-based and Cd-free QD by using these two methods and explores their potential for biophotonics applications. First, this thesis presents a facile aqueous synthesis method for preparing the biofunctionalized Cd-based QDs. The CdTe QD nanocrystals were fabricated in a microfluidic chip by using protein molecules as coordination ligands. Compared to the conventional bench-top method, the microfluidic synthesis approach produced the CdTe QDs with enhanced stability and improved biofunctionalization efficiency. Another method of preparing the aqueous QDs, phase transfer of the QDs synthesized in organic solvents, was also examined. Hydrophilic ligands with different chain lengths and functional groups were applied to render the QDs water-dispersible by using the ligand exchange method. Each set of QDs capped with specific surface ligands displayed strengths and limitations in certain aspects of their physicochemical properties, and induced different cellular responses. To mitigate toxicity concerns in biophotonics applications, Zn-Cu-In-S QDs, which are quaternary and Cd-free QDs, were prepared using a facile aqueous synthesis method. The composition effect on their optical properties is demonstrated and correlated with the evolution of their structure and energy band gap. Using the same strategy of composition control, Zn-Ag-In-S QDs with different emission wavelengths were synthesized in the organic phase and then transferred into the aqueous phase via the encapsulation method. The resulting water-dispersible ZAIS QDs exhibited bright photoluminescence and long fluorescence lifetime and were applied in temporally multiplexed imaging of cells. Finally, ZnO QDs, a type of Cd-free QDs made from wide-band-gap oxides, were prepared using the sol-gel method. Their optical properties were modified via chemical doping and photodoping techniques. Their redox-sensitive photoluminescence and plasmonic properties make them promising candidates for biosensing applications. In summary, this thesis covers the manipulation of optical properties, surface chemistry and biocompatibility of Cd-based and Cd-free QDs using versatile strategies in synthesis and surface modification processes, and demonstrates the potential of using these QDs in biophotonics applications. Doctor of Philosophy (EEE)
- Published
- 2020
25. Microfluidic chip enabled one-step synthesis of biofunctionalized CuInS
- Author
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Siyi, Hu, Butian, Zhang, Shuwen, Zeng, Liwei, Liu, Ken-Tye, Yong, Hanbin, Ma, and Yuguo, Tang
- Subjects
Zinc Compounds ,Microfluidics ,Quantum Dots ,Sulfides - Abstract
Biofunctionalized quantum dots (QDs) are effective target fluorescent labels for bioimaging. However, conventional synthesis of biofunctionalized I-III-VI core-shell CuInS2/ZnS QDs requires complex bench-top operations, resulting in limited product performance and variety, and is not amenable to a 'one-step' approach. In this work, we have successfully demonstrated a fully automated method for preparing denatured bovine serum albumin (dBSA)-CuInS2/ZnS QDs by introducing microfluidic (MF) chips to synthesize biofunctionalized QDs, hence establishing a 'one-step' procedure. We have also studied and optimized the reaction synthesis parameters. The emission wavelength of the dBSA-CuInS2/ZnS QDs is located in the near-infrared range and can be tuned from 650 to 750 nm by simply varying the reaction parameters. In addition, the 'one-step'-synthesized dBSA-CuInS2/ZnS QDs have a long average fluorescence lifetime of 153.76 ns and a small particle size of 5 ± 2 nm. To demonstrate the applicability of the 'one-step'-synthesized dBSA-CuInS2/ZnS QDs in bioimaging studies, we modified the QDs with folic acid and hyaluronic acid, and then performed target bioimaging and cytotoxicity tests on macrophages, liver cancer cells and pancreatic cancer cells. The cell images show that the red emission signals originate from the QDs, which indicates that the dBSA-CuInS2/ZnS QDs prepared by the MF approach are suitable optical contrast agents for target bioimaging. This 'one-step' MF-based QD synthesis approach could serve as a rapid, cost-effective, and small-scale nanocrystal production platform for complex QD formulations for a wide range of bioapplications.
- Published
- 2020
26. LncRNA MEG3 suppressed the progression of ovarian cancer via sponging miR-30e-3p and regulating LAMA4 expression
- Author
-
Dan Wei, Yang Liu, Yangchun Xu, Butian Zhang, Lei Ding, and Lili Yu
- Subjects
Cancer Research ,Biology ,lcsh:RC254-282 ,LncRNA MEG3 ,Metastasis ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Ovarian cancer ,Genetics ,medicine ,LAMA4 ,lcsh:QH573-671 ,Gene ,030304 developmental biology ,MEG3 ,0303 health sciences ,Messenger RNA ,lcsh:Cytology ,Microarray analysis techniques ,miR-30e-3p ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Primary Research - Abstract
Background Ovarian cancer (OC) is a common female reproductive malignancy with a high mortality rate. Although LAMA4 was observed to be downregulated in OC cells, its mechanism in regulating OC metastasis is still unknown. This study aimed to investigate the effect of LAMA4 and its mechanism on OC. Methods To achieve this aim, a microarray analysis was performed to screen out the key genes involved in OC pathogenesis. Western-blot and qRT-PCR assays were also carried out to detect protein and mRNA expressions, respectively. A luciferase reporter assay was further used to confirm the direct interaction of miR-30e-3p with MEG3, and the direct interaction of miR-30e-3p with LAMA4 mRNA. Cytological experiments (CCK8, colony formation assay, wound-healing assay etc.) were then performed to explore the roles of miR-30e-3p, MEG3, and LAMA4 in OC cells. Results After carrying out microarray analysis, LAMA4 was confirmed as a key gene associated with OC pathogenesis. Research results proved that miR-30e-3p was markedly upregulated, while MEG3 and LAMA4 were noticeably downregulated in OC tissues and cells. The overexpression of LAMA4 significantly impaired the proliferation, migration, and invasion of OC cells. However, the upregulation of MEG3 increased the expression of LAMA4 by sponging miR-30e-3p, which alleviated the malignancy of OC cells. Conclusions Observations showed that forced LAMA4 overexpression could inhibit OC progression, which was regulated by MEG3 via sponging miR-30e-3p. The findings of this research could provide new insights into the mechanism by which MEG3 and LAMA4 exert their anti-oncogenic roles in OC progression. Trial registration Not applicable
- Published
- 2020
27. The effects of platelet-rich plasma injection in knee and hip osteoarthritis: a meta-analysis of randomized controlled trials
- Author
-
Qi Yang, Jiajing Zhu, Butian Zhang, Xiaojie Sun, and Yujie Dong
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,WOMAC ,Visual analogue scale ,Osteoarthritis ,Osteoarthritis, Hip ,law.invention ,Injections, Intra-Articular ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Randomized controlled trial ,law ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Hyaluronic Acid ,Randomized Controlled Trials as Topic ,030203 arthritis & rheumatology ,Ontario ,business.industry ,Platelet-Rich Plasma ,General Medicine ,Osteoarthritis, Knee ,medicine.disease ,Treatment Outcome ,Harris Hip Score ,Platelet-rich plasma ,Meta-analysis ,Physical therapy ,business - Abstract
We conducted this updated meta-analysis to evaluate the effects of PRP in patients with knee or hip OA.PubMed, Embase, and Web of Science were searched to identify randomized controlled trials (RCTs) that compared the efficacy of PRP with other intra-articular injections. The outcomes of interest included Western Ontario and McMaster (WOMAC), Knee Injury and Osteoarthritis Outcome Score (KOOS), Visual Analog Scale (VAS), Harris Hip Score (HHS), and International Knee Documentation Committee (IKDC).Twenty-four RCTs with 21 at knee OA and three at hip OA were included in this meta-analysis. The PRP injections significantly improved the WOMAC score, VAS score, IKDC score, and HHS score as compared with comparators. The WOMAC pain, stiffness, and physical function scores were also significantly better in the PRP group than in the control group. Most of the evaluated parameters that favored PRP were observed in knee OA but not in hip OA, at short-term (at 1, 2, 3, 6, 12 months) but not long-term follow-up (at 18 months), in RCTs with low risk of bias.Intra-articular PRP injection provided better effects than other injections for OA patients, especially in knee OA patients, in terms of pain reduction and function improvement at short-term follow-up. Key Points • This updated meta-analysis, based on great sample size and high-quality studies, evaluates the effects of PRP in patients with knee or hip OA. • Intra-articular PRP injection provided better effects than other injections for OA patients. • Most of the evaluated parameters that favored PRP were observed in knee OA at short term (at 1, 2, 3, 6, 12 months).
- Published
- 2020
28. Optical Phonon Behaviors of Photocharged Nanocrystals: Effects of Free Charge Carriers
- Author
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Shun Wang, Kexin Wang, Ruiheng Chang, Jing-Tao Lü, and Butian Zhang
- Subjects
Materials science ,business.industry ,Phonon ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Free carrier ,Condensed Matter::Materials Science ,symbols.namesake ,Nanocrystal ,Condensed Matter::Superconductivity ,0103 physical sciences ,symbols ,Optoelectronics ,Semiconductor nanocrystals ,General Materials Science ,Charge carrier ,Longitudinal optical ,Physical and Theoretical Chemistry ,Photonics ,010306 general physics ,0210 nano-technology ,business ,Raman spectroscopy - Abstract
For semiconductor nanocrystals (NCs), the precise knowledge of phonons in the presence of free carriers is important for understanding their electronic and photonic properties in device applications. With Raman spectroscopy, this study investigates the effects of free charge carriers on optical phonon behaviors of NCs. The adoption of the photocharging method allows us to introduce free charge carriers into NCs without inducing other side effects. In the photocharged ZnO NCs, lower longitudinal optical (LO) phonon frequencies and weaker LO overtones relative to the fundamentals were found, which was explained by the screening and band-filling effects caused by the induced free carriers. The free carrier effects on optical phonon behaviors of NCs, usually neglected in previous studies, should be taken into consideration when discussing the electronic and photonic properties of NC-based devices.
- Published
- 2018
29. Lectin inspired polymers based on the dipeptide Ser-Asp for glycopeptide enrichment
- Author
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Cao Peng, R. Z. Yu, Yi Zhang, Yuanhang Yu, Butian Zhang, Jianying Chen, and Rong Xie
- Subjects
0301 basic medicine ,Disaccharides ,Biochemistry ,Analytical Chemistry ,Microsphere ,03 medical and health sciences ,chemistry.chemical_compound ,Adsorption ,Biomimetic Materials ,Lectins ,Electrochemistry ,Animals ,Environmental Chemistry ,Fetuins ,Spectroscopy ,chemistry.chemical_classification ,Dipeptide ,biology ,Glycopeptides ,Lectin ,Serum Albumin, Bovine ,Polymer ,Silicon Dioxide ,Combinatorial chemistry ,Microspheres ,Peptide Fragments ,Glycopeptide ,030104 developmental biology ,chemistry ,biology.protein ,Cattle ,Peptides - Abstract
Lectin inspired polymers were prepared through modification of silica microspheres with Ser-Asp (SD). This functional polymer showed distinct adsorption and retention towards different disaccharides and demonstrated high-efficiency enrichment of glycopeptides.
- Published
- 2018
30. Diffusion tensor imaging of spinal microstructure in healthy adults: improved resolution with the readout segmentation of long variable echo-trains
- Author
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Shao-nan Yu, Lili Yu, Jin-lan Jiang, Meng Li, Butian Zhang, and Yi-meng Dai
- Subjects
microstructure ,Long Variable ,lcsh:RC346-429 ,cervical spinal cord ,030218 nuclear medicine & medical imaging ,White matter ,03 medical and health sciences ,0302 clinical medicine ,Developmental Neuroscience ,nerve regeneration ,diffusion tensor imaging ,gray matter ,white matter ,readout segmentation of long variable echo-train sequence ,signal-to-noise ratio ,fractional anisotropy ,neural regeneration ,Fractional anisotropy ,medicine ,Segmentation ,lcsh:Neurology. Diseases of the nervous system ,Physics ,Resolution (electron density) ,Spinal cord ,medicine.anatomical_structure ,Signal-to-noise ratio (imaging) ,030217 neurology & neurosurgery ,Research Article ,Diffusion MRI ,Biomedical engineering - Abstract
Diffusion tensor imaging plays an important role in the accurate diagnosis and prognosis of spinal cord diseases. However, because of technical limitations, the imaging sequences used in this technique cannot reveal the fine structure of the spinal cord with precision. We used the readout segmentation of long variable echo-trains (RESOLVE) sequence in this cross-sectional study of 45 healthy volunteers aged 20 to 63 years. We found that the RESOLVE sequence significantly increased the resolution of the diffusion images and improved the median signal-to-noise ratio of the middle (C4–6) and lower (C7–T1) cervical segments to the level of the upper cervical segment. In addition, the values of fractional anisotropy and radial diffusivity were significantly higher in white matter than in gray matter. Our study verified that the RESOLVE sequence could improve resolution of diffusion tensor imaging in clinical applications and provide accurate baseline data for the diagnosis and treatment of cervical spinal cord diseases.
- Published
- 2017
31. Tailoring the Fluorescent and Electronic Properties of 2H-MoS2 by Step-by-Step Functionalization.
- Author
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Changjiang Nie, Mingming Yin, Ye Zhao, Chuanwen Zhao, Butian Zhang, Xinxin Song, Xin Yi, Youwei Zhang, Liang Luo, and Shun Wang
- Published
- 2021
- Full Text
- View/download PDF
32. The inhibitory function of icariin in cell model of benign prostatic hyperplasia by upregulation of miR-7
- Author
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Ning, Han, Butian, Zhang, Xin, Wei, and Lili, Yu
- Abstract
In spite of a considerable breakthrough in antitumor effects of icariin (ICA), the studies regarding other functions of ICA still have not explored widely. This study aimed to report the function of ICA about the growth and migration of benign prostatic hyperplasia epithelial cell line (BPH-1) and human prostate epithelial cell line RWPE-1. Both BPH-1 and RWPE-1 cells were treated with ICA for 12 hr. The cells proliferation, migration, apoptosis were tested by CCK-8 assay, transwell assay, flow cytometry, and western blot. The miR-7 expression was silenced by transfection in both BPH-1 and RWPE-1 cells. The activation of PI3K/AKT and Raf1/ERK1/2 signaling pathways was detected by western blot. ICA inhibited both BPH-1 and RWPE-1 cells proliferation, migration, and promoted apoptosis. Meanwhile, ICA promoted miR-7 expression. Other than this, the changes of cells proliferation inhibition, migration inhibition and apoptosis promotion in both BPH-1 and RWPE-1 cells induced by ICA were reversed partially or completely by transfecting specific miR-7 inhibitor. Apart from this, the activation of PI3K/AKT and Raf1/ERK1/2 signaling pathways was inhibited by ICA through upregulating miR-7 expression. The study released that ICA inhibited both BPH-1 and RWPE-1 cells proliferation, migration and promoted apoptosis by upregulating the levels of miR-7 expression, which was related to PI3K/AKT and Raf1/ERK1/2 signaling pathways.
- Published
- 2019
33. The lncRNA SNHG3 accelerates papillary thyroid carcinoma progression via the miR-214-3p/PSMD10 axis
- Author
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Guoqing Sui, Dan Fei, Feng Guo, Butian Zhang, Qiang Luo, and Hui Wang
- Subjects
0301 basic medicine ,Male ,Proteasome Endopeptidase Complex ,endocrine system diseases ,Physiology ,Carcinogenesis ,Clinical Biochemistry ,Mice, Nude ,medicine.disease_cause ,Cell Line ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Cell Movement ,Proto-Oncogene Proteins ,microRNA ,medicine ,Animals ,Humans ,Thyroid Neoplasms ,Small nucleolar RNA ,RNA, Small Interfering ,miR-214 ,Cell Proliferation ,Mice, Inbred BALB C ,Competing endogenous RNA ,Chemistry ,Cell Biology ,Middle Aged ,Long non-coding RNA ,Reverse transcription polymerase chain reaction ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Thyroid Cancer, Papillary ,030220 oncology & carcinogenesis ,Cancer research ,Disease Progression ,PSMD10 ,Female ,RNA, Long Noncoding - Abstract
Small nucleolar RNA host gene 3 (SNHG3) is a long noncoding RNA (lncRNA), which is known to promote oncogenesis in many cancers but its role in human papillary thyroid carcinoma (PTC) remains poorly understood. We therefore assessed SNHG3 expression in PTC tissues via quantitative reverse transcription polymerase chain reaction. We additionally knocked down SNHG3 in PTC cells using short-hairpin RNAs (shRNAs) to explore its functional roles in PTC. The ability of SNHG3 to bind to specific microRNAs (miRNAs) was predicted using a bioinformatics tool, and this binding was confirmed via dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. We then used a tumor xenograft model to assess the relevance of SNHG3 in vivo. We determined SNHG3 expression to be elevated in PTC tissues relative to controls, with advanced tumor-node-metastasis stage and lymph node metastasis being associated with this expression. Knocking down SNHG3 significantly reduced in vitro PTC cell migration, invasion, proliferation, and colony formation, and it further slowed the growth of tumors in vivo. We found that SNHG3 could bind to miR-214-3p as a competing endogenous RNA (ceRNA) for this miRNA, thereby regulating proteasome 26S subunit non-ATPase 10 (PSMD10) expression, a miR-214-3p target. These results thus indicate that SNHG3 is an oncogenic lncRNA in PTC, acting at least in part via the miR-214-3p/PSMD10 axis.
- Published
- 2019
34. Corrigendum to 'LINC01116 targets miR-520a-3p and affects IL6R to promote the proliferation and migration of osteosarcoma cells through the Jak-stat signaling pathway' [Biomed. Pharmacother. 107 (2018) 270–282]
- Author
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Shuang Wang, Lili Yu, Butian Zhang, Ning Han, Jin-lan Jiang, Lei Ding, and Zhenzhen Hu
- Subjects
Pharmacology ,medicine ,Cancer research ,Osteosarcoma ,JAK-STAT signaling pathway ,General Medicine ,Therapeutics. Pharmacology ,RM1-950 ,Biology ,medicine.disease - Published
- 2019
35. New Generation Cadmium-Free Quantum Dots for Biophotonics and Nanomedicine
- Author
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Butian Zhang, Shuwen Zeng, Paras N. Prasad, Gaixia Xu, Ken-Tye Yong, Mark T. Swihart, School of Electrical and Electronic Engineering, and CNRS International NTU THALES Research Alliances
- Subjects
Optics and Photonics ,Photoluminescence ,cadmium-free quantum dots ,Photoacoustic imaging in biomedicine ,Biocompatible Materials ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Optical imaging ,Metals, Heavy ,Quantum Dots ,Animals ,Particle Size ,Metalloids ,Chemistry ,biophotonics ,General Chemistry ,Photothermal therapy ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Biophotonics ,Optical tracking ,Nanomedicine ,Quantum dot ,Graphite ,0210 nano-technology - Abstract
This review summarizes recent progress in the design and applications of cadmium-free quantum dots (Cd-free QDs), with an emphasis on their role in biophotonics and nanomedicine. We first present the features of Cd-free QDs and describe the physics and emergent optical properties of various types of Cd-free QDs whose applications are discussed in subsequent sections. Selected specific QD systems are introduced, followed by the preparation of these Cd-free QDs in a form useful for biological applications, including recent advances in achieving high photoluminescence quantum yield (PL QY) and tunability of emission color. Next, we summarize biophotonic applications of Cd-free QDs in optical imaging, photoacoustic imaging, sensing, optical tracking, and photothermal therapy. Research advances in the use of Cd-free QDs for nanomedicine applications are discussed, including drug/gene delivery, protein/peptide delivery, image-guided surgery, diagnostics, and medical devices. The review then considers the pharmacokinetics and biodistribution of Cd-free QDs and summarizes current studies on the in vitro and in vivo toxicity of Cd-free QDs. Finally, we provide perspectives on the overall current status, challenges, and future directions in this field. MOE (Min. of Education, S’pore) Accepted version
- Published
- 2016
36. Biodegradable charged polyester-based vectors (BCPVs) as an efficient non-viral transfection nanoagent for gene knockdown of the BCR–ABL hybrid oncogene in a human chronic myeloid leukemia cell line
- Author
-
Ken-Tye Yong, Butian Zhang, Peter Han Joo Chong, Nishtha Panwar, Yucheng Wang, Chih-Kuang Chen, Chengbin Yang, Hui Ting Toh, Maixian Liu, Ho Sup Yoon, Wing Cheung Law, and Swee Chuan Tjin
- Subjects
0301 basic medicine ,Small interfering RNA ,Polyesters ,Genetic Vectors ,Fusion Proteins, bcr-abl ,Apoptosis ,02 engineering and technology ,Biology ,Transfection ,Philadelphia chromosome ,03 medical and health sciences ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,hemic and lymphatic diseases ,Gene Knockdown Techniques ,medicine ,Humans ,General Materials Science ,RNA, Small Interfering ,Gene knockdown ,021001 nanoscience & nanotechnology ,medicine.disease ,Molecular biology ,Cell biology ,030104 developmental biology ,Cell culture ,K562 Cells ,0210 nano-technology ,K562 cells ,Chronic myelogenous leukemia - Abstract
First-line therapy of chronic myelogenous leukemia (CML) has always involved the use of BCR-ABL tyrosine-kinase inhibitors which is associated with an abnormal chromosome called Philadelphia chromosome. Although the overall survival rate has been improved by the current therapeutic regime, the presence of resistance has resulted in limited efficacy. In this study, an RNA interference (RNAi)-based therapeutic regime is proposed with the aim to knockdown the BCR-ABL hybrid oncogene using small interfering RNA (siRNA). The siRNA transfection rates have usually been limited due to the declining contact probability among polyplexes and the non-adherent nature of leukemic cells. Our work aims at addressing this limitation by using a biodegradable charged polyester-based vector (BCPV) as a nanocarrier for the delivery of BCR-ABL-specific siRNA to the suspension culture of a K562 CML cell line. BCR-ABL siRNAs were encapsulated in the BCPVs by electrostatic force. Cell internalization was facilitated by the BCPV and assessed by confocal microscopy and flow cytometry. The regulation of the BCR-ABL level in K562 cells as a result of RNAi was analyzed by real-time polymerase chain reaction (RT-PCR). We observed that BCPV was able to form stable nanoplexes with siRNA molecules, even in the presence of fetal bovine serum (FBS), and successfully assisted in vitro siRNA transfection in the non-adherent K562 cells. As a consequence of downregulation of BCR-ABL, BCPV-siRNA nanoplexes inhibited cell proliferation and promoted cell apoptosis. All results were compared with a commercial transfection reagent, Lipofectamine2000™, which served as a positive control. More importantly, this class of non-viral vector exhibits biodegradable features and negligible cytotoxicity, thus providing a versatile platform to deliver siRNA to non-adherent leukemia cells with high transfection efficiency by effectively overcoming extra- and intra-cellular barriers. Due to the excellent in vitro transfection results from BCPV-siRNA, a newly developed biodegradable transfection agent, BCPV, is being probed for transfection performance in an animal model.
- Published
- 2016
37. Retraction
- Author
-
Ning Han, Butian Zhang, Xin Wei, and Lili Yu
- Subjects
0301 basic medicine ,medicine.diagnostic_test ,Chemistry ,Clinical Biochemistry ,General Medicine ,Transfection ,Hyperplasia ,urologic and male genital diseases ,medicine.disease ,Biochemistry ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Western blot ,Downregulation and upregulation ,Apoptosis ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,Molecular Medicine ,Signal transduction ,Protein kinase B ,PI3K/AKT/mTOR pathway - Abstract
In spite of a considerable breakthrough in antitumor effects of icariin (ICA), the studies regarding other functions of ICA still have not explored widely. This study aimed to report the function of ICA about the growth and migration of benign prostatic hyperplasia epithelial cell line (BPH-1) and human prostate epithelial cell line RWPE-1. Both BPH-1 and RWPE-1 cells were treated with ICA for 12 hr. The cells proliferation, migration, apoptosis were tested by CCK-8 assay, transwell assay, flow cytometry, and western blot. The miR-7 expression was silenced by transfection in both BPH-1 and RWPE-1 cells. The activation of PI3K/AKT and Raf1/ERK1/2 signaling pathways was detected by western blot. ICA inhibited both BPH-1 and RWPE-1 cells proliferation, migration, and promoted apoptosis. Meanwhile, ICA promoted miR-7 expression. Other than this, the changes of cells proliferation inhibition, migration inhibition and apoptosis promotion in both BPH-1 and RWPE-1 cells induced by ICA were reversed partially or completely by transfecting specific miR-7 inhibitor. Apart from this, the activation of PI3K/AKT and Raf1/ERK1/2 signaling pathways was inhibited by ICA through upregulating miR-7 expression. The study released that ICA inhibited both BPH-1 and RWPE-1 cells proliferation, migration and promoted apoptosis by upregulating the levels of miR-7 expression, which was related to PI3K/AKT and Raf1/ERK1/2 signaling pathways.
- Published
- 2019
38. Intracerebral schwannoma: A case report and literature review
- Author
-
Dongyuan Li, Weidong Yu, Yufei Gao, Daju Sun, Yu Hu, Liu Naijie, Libo Sun, Xingyi Jin, Conghai Zhao, Butian Zhang, and Zhigang Qin
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,Oncogene ,business.industry ,Cancer ,Articles ,Schwannoma ,Cell cycle ,medicine.disease ,Molecular medicine ,Lesion ,03 medical and health sciences ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,otorhinolaryngologic diseases ,medicine ,medicine.symptom ,business ,neoplasms ,030217 neurology & neurosurgery ,Rare disease - Abstract
Intracranial schwannoma accounts for between 5 and 8% of intracranial tumors, whereas intracerebral schwannoma, a rare disease, accounts for 40. The prognosis was classified as good (patient can live independently) for the majority of patients if surgery was able to completely resect the lesion.
- Published
- 2018
39. The composition effect on the optical properties of aqueous synthesized Cu–In–S and Zn–Cu–In–S quantum dot nanocrystals
- Author
-
Liwei Liu, Ken-Tye Yong, Butian Zhang, Yucheng Wang, Siyi Hu, Yue Wang, Yuan Gao, Hilmi Volkan Demir, Yiping Zhang, and Chengbin Yang
- Subjects
Photoluminescence ,Nanostructure ,Materials science ,Cell Survival ,Band gap ,Analytical chemistry ,Water ,General Physics and Astronomy ,Indium ,Cell Line ,Blueshift ,Mice ,Zinc ,Microscopy, Electron, Transmission ,Microscopy, Fluorescence ,Nanocrystal ,Quantum dot ,Absorption band ,Quantum Dots ,Animals ,Physical and Theoretical Chemistry ,Ternary operation ,Copper ,Sulfur - Abstract
Multiternary quantum dots (QDs), because of the large degree of freedom in their structure and composition, have a wide tunability in their bandgap but also exhibit an increased uncertainty and complexity in their optical properties. In this work, we synthesized the ternary Cu-In-S (CIS) and quaternary Zn-Cu-In-S (ZCIS) QDs with different composition ratios via a facile aqueous route. The CIS QDs show multi-peak photoluminescence with their peak intensity dependent on the Cu : In ratio, which was illustrated using a donor-acceptor pair recombination process. Upon incorporation of Zn into the CIS QDs under similar conditions, the acquired ZCIS QDs exhibit blue-shifted photoluminescence (PL) spectra with an enhanced emission intensity and a narrowed spectral width (∼100 nm). A comparative study reveals that, reducing the Cu : In ratio in the CIS QDs and increasing the Zn content in the alloyed ZCIS QDs are both feasible strategies for bandgap engineering, although the influences on optical properties of the QDs were different. The XRD and EDX spectra revealed that the widening of the bandgap of the ZCIS QDs was correlated with the alloyed nanostructures and the preferential substitution of Cu by Zn. Compared to the Cu : In ratio variation, incorporation of Zn into CIS QDs is an effective strategy to achieve a more homogeneous absorption band and a wide range of emission wavelength tunability. After ZnS shell coating, the ZCIS/ZnS QDs show a further enhanced PL intensity with a prolonged fluorescence lifetime. Unlike CIS QDs, the blue shift in PL upon the shell growth was not pronounced for ZCIS QDs, for which a surface reconstruction mechanism was proposed and discussed. Finally, the as-prepared ZCIS/ZnS QDs were employed for in vitro cell imaging and exhibited good biocompatibility to macrophage cells.
- Published
- 2015
40. Interleukin-8 gene silencing on pancreatic cancer cells using biodegradable polymer nanoplexes
- Author
-
Ken-Tye Yong, Chih-Kuang Chen, Tommy Anderson, Chong Cheng, Guimiao Lin, Hui Ting Toh, Rui Hu, Ho Sup Yoon, Wing Cheung Law, Butian Zhang, Chengbin Yang, and Quoc Toan Nguyen
- Subjects
Small interfering RNA ,Tertiary amine ,Genetic enhancement ,Biomedical Engineering ,Transfection ,Biology ,medicine.disease ,Molecular biology ,RNA interference ,Pancreatic cancer ,Gene expression ,Cancer research ,medicine ,Gene silencing ,General Materials Science - Abstract
Pancreatic cancer is one of the deadliest cancers throughout the world with rarely efficient therapies currently available. Gene therapy on pancreatic cancer through small interfering RNA (siRNA)-based RNA interference (RNAi) has shown great potential and attracted much attention. However, due to the fragile nature of nucleic acid, the application of RNAi as a safe and efficient carrier faces great challenges. In this contribution, a self-assembly regime, which is based on well-defined cationic polylactides (CPLAs) with tertiary amine groups, has been used to encapsulate and protect siRNAs from fast degradation. CPLA is a safe and degradable formulation that allowed us to deliver siRNAs targeting the proangiogenic chemokine interleukin-8 (IL-8) to pancreatic cancer cells for gene therapy. Stable IL-8 siRNA-CPLA nanoplexes were successfully formed by electrostatic force and high gene transfection efficiencies were shown on two pancreatic cancer cell lines. We did not observe any cytotoxicity from these CPLAs over a large concentration range via cell viability evaluations. More importantly, the silencing of IL-8 gene expression significantly attenuated the proliferation of pancreatic cancer cells. Our preliminary results support the future development of gene therapy that might provide an effective and safe treatment approach towards pancreatic cancer.
- Published
- 2014
41. Thickness-Dependent Enhancement of Electronic Mobility of MoS2 Transistors via Surface Functionalization.
- Author
-
Changjiang Nie, Butian Zhang, Yuting Gao, Mingming Yin, Xin Yi, Chuanwen Zhao, Youwei Zhang, Liang Luo, and Shun Wang
- Published
- 2020
- Full Text
- View/download PDF
42. Cytotoxicity assessment of functionalized CdSe, CdTe and InP quantum dots in two human cancer cell models
- Author
-
Jing Liu, Liwei Liu, Jianwei Liu, Ling Ye, Rui Hu, Butian Zhang, Ken-Tye Yong, Yucheng Wang, Xin Liu, and Wing Cheung Law
- Subjects
Materials science ,Cell Survival ,Phosphines ,Cell ,Bioengineering ,Nanotechnology ,Apoptosis ,Indium ,Biomaterials ,Cell Line, Tumor ,Quantum Dots ,Toxicity Tests ,medicine ,Cadmium Compounds ,Humans ,Cytotoxicity ,Selenium Compounds ,Dose-Response Relationship, Drug ,Neoplasms, Experimental ,Poloxamer ,Cadmium telluride photovoltaics ,medicine.anatomical_structure ,Nanocrystal ,Mechanics of Materials ,Quantum dot ,Toxicity ,Tellurium ,Human cancer - Abstract
The toxicity of quantum dots (QDs) has been extensively studied over the past decade. Some common factors that originate the QD toxicity include releasing of heavy metal ions from degraded QDs and the generation of reactive oxygen species on the QD surface. In addition to these factors, we should also carefully examine other potential QD toxicity causes that will play crucial roles in impacting the overall biological system. In this contribution, we have performed cytotoxicity assessment of four types of QD formulations in two different human cancer cell models. The four types of QD formulations, namely, mercaptopropionic acid modified CdSe/CdS/ZnS QDs (CdSe-MPA), PEGylated phospholipid encapsulated CdSe/CdS/ZnS QDs (CdSe-Phos), PEGylated phospholipid encapsulated InP/ZnS QDs (InP-Phos) and Pluronic F127 encapsulated CdTe/ZnS QDs (CdTe-F127), are representatives for the commonly used QD formulations in biomedical applications. Both the core materials and the surface modifications have been taken into consideration as the key factors for the cytotoxicity assessment. Through side-by-side comparison and careful evaluations, we have found that the toxicity of QDs does not solely depend on a single factor in initiating the toxicity in biological system but rather it depends on a combination of elements from the particle formulations. More importantly, our toxicity assessment shows different cytotoxicity trend for all the prepared formulations tested on gastric adenocarcinoma (BGC-823) and neuroblastoma (SH-SY5Y) cell lines. We have further proposed that the cellular uptake of these nanocrystals plays an important role in determining the final faith of the toxicity impact of the formulation. The result here suggests that the toxicity of QDs is rather complex and it cannot be generalized under a few assumptions reported previously. We suggest that one have to evaluate the QD toxicity on a case to case basis and this indicates that standard procedures and comprehensive protocols are urgently needed to be developed and employed for fully assessing and understanding the origins of the toxicity arising from different QD formulations.
- Published
- 2014
43. Arsenic incorporation into InGaAsP grown by low-pressure metalorganic vapor phase epitaxy using tertiarybutylarsine and tertiarybutylphosphine in N[sub 2] ambient
- Author
-
Xiaohong Tang, Butian Zhang, G. S. Huang, and Swee Chuan Tjin
- Subjects
Alloy ,Kinetics ,Inorganic chemistry ,General Physics and Astronomy ,chemistry.chemical_element ,Chemical vapor deposition ,engineering.material ,Epitaxy ,Nitrogen ,Gallium arsenide ,chemistry.chemical_compound ,chemistry ,engineering ,Metalorganic vapour phase epitaxy ,Arsenic - Abstract
InxGa1−xAsyP1−y epilayers have been grown by low-pressure metalorganic vapor phase epitaxy (LPMOVPE) using tertiarybutylarsine (TBA) and tertiarybutylphosphine (TBP) as group V precursors and nitrogen as the carrier gas. Arsenic incorporation into InxGa1−xAsyP1−y films grown by LPMOVPE as a function of the gas phase composition ratio and V/III ratio has been systematically studied. With optimized growth conditions, the arsenic composition of the epilayers does not change linearly with the TBA source flow. It is observed that the arsenic incorporation becomes saturated when the gas phase composition TBA/(TBA+TBP) increases to 0.4. The incorporation kinetics in MOVPE growth of InxGa1−xAsyP1−y alloy has been analyzed by using an adsorption-trapping model. The As composition (y) of the InxGa1−xAsyP1−y films varies with the TBA gas phase composition ξ=TBA/(TBA+TBP) according to the expression y=2Ns*/aN0(1−e−θβξ). It is demonstrated that with the optimized growth conditions, TBA has a higher incorporation effic...
- Published
- 2003
44. Revisiting the principles of preparing aqueous quantum dots for biological applications: the effects of surface ligands on the physicochemical properties of quantum dots.
- Author
-
Butian Zhang, Rui Hu, Yucheng Wang, Chengbin Yang, Xin Liu, and Ken-Tye Yong
- Published
- 2014
- Full Text
- View/download PDF
45. Comments on the Translation Series on the Origins and Development of Science.
- Author
-
Butian, ZHANG
- Published
- 2013
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