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Background The efficacy of bariatric surgery may be in part attributed to altered metabolism via new gut microbiome. Milkfat may promote the growth of microbes that are beneficial in long-term weight loss. Understanding the specific gut microbiome changes after surgery and their relationship to milkfat consumption may yield important strategies for managing obesity after bariatric procedures. Methods In this pilot study, stool samples were collected from nine patients before and at the time of surgery, and at 1, 3, and 6 months post-surgery. At each time-point, dairy consumption was determined from dietary surveys. 16 s rRNA gene sequencing was performed followed by alpha diversity analysis. Comparisons of relative abundances of microbial taxa and analyses of fatty acids changes were performed. Results Bariatric surgery led to enrichment of (i) Roseburia, associated with weight loss and (ii) Christensenellaceae, inversely related to body mass index. High milk-fat consumption correlated with enrichment of Blautia, inversely associated with visceral fat accumulation. Faecalibacterium, possibly associated with obesity, increased in patients with low milk-fat consumption. Butter was associated with decreased alpha diversity in all subjects (p-value = 0.038) and the frequency of its use was associated with decreased alpha diversity in patients (correlation = − 0.68, p-value = 0.042). Low-milk-fat consumers showed higher concentration of saturated fatty acids. Conclusions Our results suggest that incorporating dairy products in post-bariatric-surgery dietary plans may help cultivate a gut microbiome that is effective in regulating fat storage as well as digesting beneficial metabolites. These observations will be helpful for the management of obesity in general population as well. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s11695-021-05805-z.

En las ovejas de pelo existe muy poca información respecto a las características de la producción y calidad de la leche a lo largo de la lactancia, pese a sus implicaciones tanto en la sobrevivencia y mantenimiento de los corderos, como en la alimentación humana. Con el propósito de caracterizar la curva de lactancia y la calidad de la leche en ovejas Santa Cruz, se utilizaron 18 ovejas multíparas que parieron en un lapso de cuatro días. La producción láctea se registró cada 72 h en el periodo comprendido de 6 a 60 días postparto (dpp), mientras que la calidad de la leche se determinó una vez por semana de una muestra de la producción total del día. La producción de leche 6 dpp fue de 1.95 L, alcanzando un máximo de 2.31 L, 12 dpp y disminuyendo hasta 1.01, a los 57 dpp. El porcentaje de sólidos totales fue 18 %, incrementando hasta 20.5 % en la octava semana. El porcentaje de grasa durante la segunda semana fue 8 % incrementándose hasta 9.8 % al final del periodo evaluado, mientras que los porcentajes de proteína y lactosa se mantuvieron relativamente uniformes durante todo el periodo experimental en niveles entre 4.86 y 5.18 % para proteína, y 4.68 a 4.74 % para lactosa. Se concluye que la producción de leche en ovejas Santa Cruz, alcanza su máximo alrededor de la segunda semana de lactancia, disminuyendo a partir de ese momento de manera constante y paulatina, incrementando los porcentajes de sólidos totales y grasa a través del tiempo, mientras que los de proteína y lactosa se mantienen constantes.

The lipolytic behaviors and postprandial effects of butterfat and its fractions (30L and 30S) procured by dry fractionation at 30 °C were investigated using in vivo digestion. A total of 142 triacylglycerols (TAGs), 64 diacylglycerols (DAGs), 14 monoacylglycerols (MAGs), and 7 free fatty acids (FFAs) in the butterfat fractions and their hydrolysates were identified by combining high-performance liquid chromatography coupled with electrospray ionization-tandem quadrupole time-of-flight mass spectrometry with solid-phase extraction. The first-step hydrolysis from TAGs to sn-1,2 DAGs occurred slower in the high-melting-temperature solid fat (30S) fraction, which is rich in long-chain FAs compared to that of the low-melting-temperature liquid oil (30L) fraction, which is rich in short-chain unsaturated FAs (the hydrolysis rates were 39.22% vs 60.11%, respectively, in the 30 min gastric phase), and these differences were also reflected in the delayed and relatively flat postprandial lipemia levels in rats force-fed with 30S fraction. This study revealed the importance of TAG composition and lipid physical state in regulating digestion and absorption, which is related to nutrition science and the dairy or pharmaceutical industry.

High-melting-temperature solid triacylglycerol (TAG) is the main source of controversy with regard to the nutritional assessment of milk fat. This study investigated the microscopic changes and hydrolysis kinetics of milk fat globules (MFGs) reconstituted with butterfat and its primary fractions (30S, 20S, and 20L) during in vitro digestion. The 30S, 20S, and 20L on behalf of high-, medium- and low-melting-temperature fractions, respectively, had well-distinguished melting temperatures (42.1, 38.9, and 22.0 °C) and long-chain saturated TAG contents (19.3, 3.2, and 1.8%). The results revealed that the gastrointestinal fate of these butterfat fractions varied greatly with their TAG composition, and the gastric phase was a sensitive target in terms of the physiological site. The 20S- and 30S-reconstituted MFG emulsions during gastric digestion compared to that of 20L had higher extensive aggregation, lower hydrolysis extent (29.8, 28.0, and 57.3%, respectively), and slower apparent hydrolysis rate constants k (2.4, 2.1, and 6.1 min-1, respectively).

In this research, Sanliurfa butterfat, collected from local small-sized dairy plants located in Karacadağ Region of Sanliurfa, were stored at two different temperatures (4 and 20 °C) for 12 months. The butterfat samples have been analysed to determine some biochemical parameters (free fatty acids, acid value, peroxide value, induction time, and pH) and fatty acid composition on the 1st, 30th, 90th, 120th, 180th, 270th and 360th days of storage. The mean value of free fatty acids, acid value and peroxide value of the butterfat stored in refrigerator conditions (4 °C) were lower, and the induction time was higher than the butterfat stored at room temperature (20 °C) (P

Twelve lactating sahelian goals aged between 2 and 3 years and averaging 25kg body weight were selected from the herd of the University of Maiduguri Teaching and Research Farm, for a 19-week lactation study. The does were classified into early mid and late lactation stages and maintained daily on 1kg concentrate mixture per animal in addition to grazing on Gambia Grass. Milk collected were analysed for total solids (TS), butterfat, protein, solids-non-fat (SNF), Ash and energy. The mean percentage values (M+SE) of colostrum were TS 14.96 ± 0.41; butterfat 8.58±1.0; protein 5.64±0.31; ash 0.93±0.07; SNF 6.37±0.51; and energy 3.21 ± 0.39 MJ/kg. The overall mean values for mature milk were 13.18±0.27, 7.52±0.18, 4.81±0.14, 0.76±0.03, 5.66±0.16% and 2.79±0.12 MJ/KG respectively for TS. fat, protein, ash SNF and energy. There was a gradual decline in the values of all the components of colostrum from day 1-6 after kidding. Generally, colostrum constituents were higher in concentration than those of mature milk on the 7th day after parturition. The mature milk constituents tended to increase with advancing lactation. Milk constituents (especially Fat, Protein and SNF), as evident from this study could be manipulated through feeding.

Milk was once obtained daily for 98 days from Muturu cows in their 3r lactation and analysed for yield and composition. Results showed that milk yield was generally low but there were highly significant (P < 0.01) individual variations in milk output. The butter fat content of the milk appeared relatively high (4.72 +0.08%). However, all the milk constituents were within the values reported for other tropical breeds. There was a significant inverse relationship (r = -0.87; P

An analysis of the productive qualities of mothers cows and their offspring, obtained from different fathers, showed that the contribution of parents to the formation of milk productivity is unequal. Intensive selection of bulls-producers leads to fixation of homozygotes at the loci associated with traits of milk production, which increases their influence on offspring. The strength of the influence of the father’s factor on milk yield, milk protein and live weight of daughters was 6, respectively; 14 and 16%. The productivity level of mothers did not significantly affect the productivity of their daughters. High-yielding offspring can be obtained from low-yielding cows. Daughters surpassed their mothers in milk yield, but inferior in fat milk. The proportion of deviation from the theoretically expected productivity of daughters (milk yield per 305 days), which is caused by the influence of the maternal genotype, in the groups of Auckland No. 426436885, Showman No. 831842 and Euclid No. 831491, respectively, was 15.8; 27.5 and 30.4%. The correlation coefficient between the milk yield of mothers and daughters was + 0.25 (p (from +0.34 to +0.67). For mothers, the relationship between these indicators was lower. It is possible that half-siblings have stronger genetic links between traits than unrelated mothers. Genetic diversity in milk yield (h2 = 0.6) and live weight (h2 = 0.5) was established. Repeatability coefficients for fat and milk protein were at the level of 0.35. The productive potential of the breed depends on the combinational compatibility of the parents, their contribution to the formation of complex quantitative characteristics of productivity.

The milk yield and compositions of twelve lactating does comprising six each of West African Dwarf (Fouta djallon) and Red Sokoto (Maradi) were compared in a 21-week study. The does in their second parity were monitored over a full lactation. The goats received daily basal grass and browse fodder in addition to a concentrate supplement in a 2 × 3 factorial experiment. The does were hand milked and milk yield (g/d) was quantified daily. Milk samples were analyzed weekly for total solids (TS %), butter fat (BF %), solids-not-fat (SNF %), crude protein (CP %) and total ash (TA %). Lactose concentration (LC %) was analyzed daily immediately after sampling. Macro-minerals in milk samples were determined by dry ash extraction method specific for each mineral element. Milk yield was influenced significantly (p0.05) by breed. The TA was significantly (P

Animal milk has been an outstanding balanced food for man, and a very good source of protein to man. The bulk of milk consumed in Nigeria is imported. An experiment was conducted with twelve lactating West African dwarf does that were at their second parity using graded levels (0%, 10%, 20% and 30%) of boiled rubber seed meal to investigate their colostrum and normal milk yield and proximate compositions. The rubber seeds were boiled for 30 minutes, dehulled and dried for three days before being milled and used alongside cassava peels, brewer dried grain, palm kernel cake, bone meal and salt to formulate boiled rubber seed meal based diet. The twelve does were individually housed in a well ventilated cement floored pen with wood shaving as bedding material. 1 kg of the concentrate diet designated as A, B, C and D were randomly assigned to the four treatment groups. The diets were supplied to the does in wooden feeding trough at 0800 hour in the morning while 2 kg of Panicum maximum was fed to the does by 1400 hours daily. The does were fed with the concentrate diet at the beginning of their last month of pregnancy and through to the 55th day of post-partum. The kids were weighed on their first day of parturition. Colostrum was collected, weighed and samples stored at -5oC in a refrigerator for constituent analysis, but certain sample of colostrum was also used for lactose analysis daily. The lactating does were hand milked once in the morning and a constant 0.6596 was used as a weighing factor on the morning yield using S=M+0.6596M as correcting factor. Prior to each day milking, kids were separated from the dams from 1800 hour and fed with the aid of feeding bottle. Colostrum and normal milk samples were analysed for crude protein, (Nx6.38), lactose, total solids, solid-not-fat, butterfat, ash and energy. Lactose was analysed using Marier and Boulet procedures. Butterfat was determined by Kjeldahl and Markhaps apparatus while solid-not-fat was obtained from the differences between butterfat and total solids. Energy was computed using multiple regression equation. AOAC method was used to determine the proximate composition of the experimental diets. There was no significant differences (P0.05) in their colostrum with treatment D having superior value (6.87%) compared to A (6.58%), B (6.50%) and C (6.44%). The CP value increases as the BRSM increases in diet. All parameters in normal milk were statistically similar in their values, but treatment B (10% BRSM) expressed superior value among the treatment groups. In this study, 30%BRSM promoted least milk yield while 10%BRSM promoted highest milk yield. Therefore, 10% inclusion level of BRSM is recommended for lactating WAD does for high milk yield.

This study aimed to determine the effect of diet formulated to provide prepartum (DCAD= -749.16 mEq/head/day) and postpartum (DCAD = 1473.56 mEq/head/day) by feeding 90 g of an anionic and 120 g cationic salt respectively, on blood mineral concentration, health, postpartum milk production and composition in buffaloes. Twenty multiparous buffaloes were enrolled 21 days before expected calving date and divided into two groups. The treatment group with 10 cows received anionic rations for - 21 day to parturition and cationic ration from parturition to +21 days, while the control group was fed a usual ration. Postpartum incidences of milk fever, dystocia, retention of placenta, mastitis as well as weekly data of milk production, and milk composition were recorded. Plasma samples obtained at days -21, -10, -1, +1, +10 and +21 relative to calving were analyzed for calcium and magnesium. Magnesium concentration was increased with reduced prepartum Dietary Cation-Anion Difference (DCAD) in the diet value being 2.77±0.13 mg/dl and 3.15±0.12 mg/dl for the control and treatment group respectively, one day before calving. Calcium concentration was significantly higher (P

Use of conserved forages and proper ration formulation has great potential to bridge the gap in dairy nutrition and reduce seasonal variations in milk yield. This study determined the effects of various fodder and ration formulation interventions on dairy farm performance in North Rift, Eastern, and Central regions of Kenya. Seventy-two farms were purposively selected as participating farms and assigned into six groups of twelve as follows: two groups on silage production, two groups on ration formulation, and two control groups having similar production systems and in the same geographical locations as the other groups. Data on daily dry matter feed intake and milk yield were recorded while laboratory analysis was done to determine milk butterfat and protein content. The data was analyzed using a multi-linear regression model to assess the relationship between independent and dependent variables. The results showed that farmers using feed rationing with advisory services had the highest average daily milk yield (19.7 kg/cow) compared to maize train silage (16.8 kg/cow) and those with silage support from Service Provider Enterprises (SPE) (13.3 kg/cow) (P0.05) across the interventions. In conclusion, use of maize train silage and feed rationing with advisory services increased milk yield and reduced seasonal milk fluctuation. Int. J. Agril. Res. Innov. Tech. 10(2): 76-83, December 2020

Modification of dairy products’ fat composition or replacement by other fats are common practices in the industry. Products in which milkfat is partially or wholly substituted by vegetable fats are defined as dairy analogues. This study delivers gap-filling information about sour cream analogues in nutritional aspects, focusing on the fat-related attributes. Analogue sour creams contained 29.6% less saturated fatty acids, 88.4% less cholesterol and 88.7% more mono- and poly-unsaturated fatty acids than the sour creams. Trans fatty acids were present in both products, which could not be linked to artificial hydrogenation in either case. Digestibility of the examined samples might be different, as sour creams had more short and medium chain fatty acids than the analogues. Overall, sour cream analogues, besides serving as an alternative to sour cream, have particular advantages based on their nutritional values.

In this study, the composition, fat indices, color, biochemical and textural parameters of Şanlıurfa butterfat and Leather bag fat, which are produced in small dairy farms in the Karacadağ region of Şanlıurfa, were investigated. As a result of the evaluation, the average value of fat ratio, iodine number, Polenske number, free acidity, acid value and peroxide value of Leather bag fat were higher, whereas the average value of moisture content, cholesterol amount, water activity, L * value and induction time of the fat were lower than Şanlıurfa butterfat (P, Bu çalışmada, Şanlıurfa ili Karacadağ bölgesinde küçük süt işletmelerinde üretilen Şanlıurfa sadeyağı ile Tulum yağının (Runı hiz) bileşimi, bazı yağ indisleri, renk, biyokimyasal ve tekstürel parametreleri araştırılmıştır. Yapılan istatistiksel değerlendirmede, Şanlıurfa sadeyağına oranla, Tulum yağının ortalama yağ oranı, iyot sayısı, serbest asitlik, asit değeri ve peroksit değeri yüksek, nem oranı, kolesterol miktarı, su aktivitesi, L* değeri ve indüksiyon süresi ise daha düşük bulunmuştur (P

Aged cheese is an increasingly popular dairy product. One approach to reduce Cheddar cheese maturation time is by utilizing elevated temperature, despite potential problems including development of imbalanced or off-flavors and negative changes in texture. Therefore, the objective of this study was to evaluate the influence of elevated ripening temperature on chemical and sensory properties of aged white Cheddar cheese. White Cheddar cheese was aged at 7.2, 10, or 12.8 °C for 12 months, with samples evaluated at 2, 5, 8, 10, 11, and 12 months by a trained sensory panel (n = 10). Two consumer sensory panels (n = 120) assessed 8- and 12-month aged cheese for comparison to a commercially available reference sample of the same cheese, aged for 12 months. An electronic tongue methodology was developed for analysis of nonvolatile compounds. Trained panel results showed that 2-month cheeses were described by milkfat flavor and sweet taste, 5-month cheeses were described by nutty aroma and white color, and 8-, 10-, 11-, and 12-month cheeses developed aged characteristics, such as umami and bitter tastes, brothy aroma, and aged flavor. Consumer panel results showed similar overall liking scores for the reference cheese and cheeses aged at 10 or 12.8 °C for both evaluations. The electronic tongue could classify samples according to aging month with a validity value of 92.59%. In conclusion, the electronic tongue served as a valid method of instrumental analysis for Cheddar cheese samples throughout maturation. This study demonstrated that aging white Cheddar cheese for 8 months at an elevated storage temperature of 10 °C produced cheese similar in consumer acceptance to that aged at 7 °C for 12 months. PRACTICAL APPLICATION: This study showed that aged white Cheddar storage at a higher temperature was perceived similarly by consumers as one stored for 1 year at a slightly lower temperature. This may be useful to those in the dairy industry exploring ways to accelerate aging, reducing devoted resources, while still producing an acceptable product. Also, the electronic tongue was effective at distinguishing among aged white Cheddar cheese samples showing another application for this technology.

Enzymatic interesterification gives the possibility to obtain a wide range of lipids with a modified structure. In the present study, model meat batters were produced from chicken breast muscles and enzymatically interesterified fats: milkfat:rapeseed oil (3:2 w/w). Fatty acids composition and their positional distribution in triacylglycerol, melting profile and oxidative stability have been determined in fats used for interesterification, after interesterification and extracted from meat batters. In meat batters, the physiochemical parameters were determined. Interesterified fats were characterized by significantly lower induction times than noninteresterified fat (85.29 and 18.21 min, respectively). Meat batters were also characterized by a lower oxidative stability of lipid fraction (24.90 and 13.67 min) than lipids used to their production. A higher content of unsaturated fatty acids was found in meat batters with noninteresterified and interesterified fats (69.40% and 70.03%, respectively) than in the control meat batter with a pork jowl (58.63%). In comparison to the control product, meat batter prepared with interesterified mixture was characterized by significantly lower apparent viscosity. In the analyzed meat batters, there were no differences in thermal drip and penetration force. The incorporation of interesterified milkfat with rapeseed oil in model meat batters can be a strategy to improve the nutritional quality without adversely affecting the quality characteristics.

Gestational high butterfat (HFB) and/or endocrine disruptor exposure was previously found to disrupt spermatogenesis in adulthood. This study addresses the data gap in our knowledge regarding transgenerational transmission of the disruptive interaction between a high-fat diet and endocrine disruptor bisphenol A (BPA). F0 generation Sprague-Dawley rats were fed diets containing butterfat (10 kcal%) and high in butterfat (39 kcal%, HFB) with or without BPA (25 µg/kg body weight/day) during mating and pregnancy. Gestationally exposed F1-generation offspring from different litters were mated to produce F2 offspring, and similarly, F2-generation animals produced F3-generation offspring. One group of F3 male offspring was administered either testosterone plus estradiol-17β (T + E2) or sham via capsule implants from postnatal days 70 to 210. Another group was naturally aged to 18 months. Combination diets of HFB + BPA in F0 dams, but not single exposure to either, disrupted spermatogenesis in F3-generation adult males in both the T + E2-implanted group and the naturally aged group. CYP19A1 localization to the acrosome and estrogen receptor beta (ERbeta) localization to the nucleus were associated with impaired spermatogenesis. Finally, expression of methyl-CpG-binding domain-3 (MBD3) was consistently decreased in the HFB and HFB + BPA exposed F1 and F3 testes, suggesting an epigenetic component to this inheritance. However, the severe atrophy within testes present in F1 males was absent in F3 males. In conclusion, the HFB + BPA group demonstrated transgenerational inheritance of the impaired spermatogenesis phenotype, but severity was reduced in the F3 generation.

The effects of wall composition and heat treatment on the formation and properties of core-in-wall emulsions (CIWEs) consisting of whey protein-coated milkfat (AMF) droplets and a dispersion of non-fat milk solids (MSNF) were investigated. Microcapsules were prepared by spray drying these CIWEs. The d3.2 of the CIWEs ranged from 0.36 to 0.54 μm. Surface excess of the CIWEs ranged from 1.39 to 6.57 mg/m2, and was influenced by concentration of whey proteins and heat treatment (30 min at 90 °C). Results indicated a preferential adsorption of β-lg at the O/W interface. Whey proteins accounted for up to 90% of the proteins adsorbed at the O/W interface. The core retention during spray drying ranged from 90.3% to 97.6% and microencapsulation efficiency ranged from 77.9% to 93.3%. The microcapsules exhibited an excellent long-term oxidative stability at 20 and 30 °C that was superior to that of microcapsules consisting of milkfat and MSNF, where the O/W interface was populated mainly by caseins. The superior oxidative stability could be attributed to the formation of dense whey-proteins-based films at the O/W interfaces of the CIWEs that isolated the core domains from the environment. The results open new opportunities in developing highly stable lipids-containing microcapsules and dairy powders.

Horses are late-ripening animals with low fecundity and therefore should be used in dairy horsebreeding for a long time to ensure the profitability of the industry. In Central Russia, mare's milk is produced fromdraft horse breeds, including Lithuanian heavy-draft horses. They are highly productive animals. With intensiveincreasing the milk yield for the first lactation, the lifetime milk yield and the period of productive use may be declined. Studies have shown that the optimal level of increased milk yield for Lithuanian draft mares is 3001-4000 kgof milk. At this level, the mares give the highest possible lifetime yield of 24,403.66 and the highest amount of milkfat and milk protein. If the increase in the milk yield for the first lactation reaches 6000 kg and above, then in maresof the studied breed the period of productive use and the lifetime yield reduce.

Our goal was to determine the effect of systematically controlled variation in milk fat, true protein, casein, and serum protein concentrations on the sensory color, flavor and texture properties, instrumental color and viscosity, and milk fat globule size distribution of milk-based beverages. Beverage formulations were based on a complete balanced 3-factor (fat, true protein, and casein as a percentage of true protein) design with 3 fat levels (0.2, 1.0, and 2.0%), 4 true protein (TP) levels (3.00, 3.67, 4.34, and 5.00%) within each fat level, and 5 casein as a percentage of true protein (CN%TP) levels (5, 25, 50, 75, and 80%) within each protein level (for a total of 60 formulations within each of 2 replicates). Instrumental measures of Hunter L and a values and Commission Internationale de l'Eclairage (CIE) b* values, instrumental viscosity, particle size, flavor, sensory texture and sensory appearance evaluations were done on each pasteurized/homogenized beverage formulation. Within each of the 3 fat levels, higher serum protein concentration drove higher aroma intensity, sweet aromatic, cooked/sulfur, cardboard/doughy flavors, and sensory yellowness scores, whereas higher casein concentration drove higher instrumental viscosity in milk protein beverages. Increasing serum protein concentration increased yellowness, sweet aromatic, aroma intensity, cooked/sulfur, and cardboard/doughy flavors across all fat levels and also had the largest effect on L, a, and b* values, sensory whiteness, and opacity within each fat level. Increases in true protein increased throat cling and astringency intensities. Increases in fat concentration were correlated with higher L, a, and b* values, larger particle size, and increased sensory whiteness, mouth coating, cooked/milky, and milkfat flavors. Multiple linear regression of L, a, and b* values produced better predictions of sensory whiteness and yellowness of pasteurized milk protein beverages than simple linear regression of L or b* values, respectively. Formulating milk protein beverages to a higher true protein level increased astringency regardless of fat level. When formulating milk protein beverages, a product developer has a wide range of milk-based protein ingredient choices that differ in price and change price relationship across time. Understanding the expected relative effect of different milk protein ingredients on the textural and flavor characteristics of milk-based beverages could be used to help guide product reformulation decisions and ingredient choices to achieve a specific sensory profile while controlling total beverage ingredient cost.

Acid whey resulting from the production of soft cheeses is a disposal problem for the dairy industry. Few uses have been found for acid whey because of its high ash content, low pH, and high organic acid content. The objective of this study was to explore the potential of recovery of whey protein from cottage cheese acid whey for use in yogurt. Cottage cheese acid whey and Cheddar cheese whey were produced from standard cottage cheese and Cheddar cheese-making procedures, respectively. The whey was separated and pasteurized by high temperature, short time pasteurization and stored at 4°C. Food-grade ammonium hydroxide was used to neutralize the acid whey to a pH of 6.4. The whey was heated to 50°C and concentrated using ultrafiltration and diafiltration with 11 polyethersulfone cartridge membrane filters (10,000-kDa cutoff) to 25% total solids and 80% protein. Skim milk was concentrated to 6% total protein. Nonfat, unflavored set-style yogurts (6.0 ± 0.1% protein, 15 ± 1.0% solids) were made from skim milk with added acid whey protein concentrate, skim milk with added sweet whey protein concentrate, or skim milk concentrate. Yogurt mixes were standardized to lactose and fat of 6.50% and 0.10%, respectively. Yogurt was fermented at 43°C to pH 4.6 and stored at 4°C. The experiment was replicated in triplicate. Titratable acidity, pH, whey separation, color, and gel strength were measured weekly in yogurts through 8 wk. Trained panel profiling was conducted on 0, 14, 28, and 56 d. Fat-free yogurts produced with added neutralized fresh liquid acid whey protein concentrate had flavor attributes similar those with added fresh liquid sweet whey protein but had lower gel strength attributes, which translated to differences in trained panel texture attributes and lower consumer liking scores for fat-free yogurt made with added acid whey protein ingredient. Difference in pH was the main contributor to texture differences, as higher pH in acid whey protein yogurts changed gel structure formation and water-holding capacity of the yogurt gel. In a second part of the study, the yogurt mix was reformulated to address texture differences. The reformulated yogurt mix at 2% milkfat and using a lower level of sweet and acid whey ingredient performed at parity with control yogurts in consumer sensory trials. Fresh liquid acid whey protein concentrates from cottage cheese manufacture can be used as a liquid protein ingredient source for manufacture of yogurt in the same factory.

Measurement of lipoprotein lipase activity (as free fatty acid change or accumulation) is accepted as indicative of milkfat globule membrane (MFGM) disruption. However, measurement is confounded by variables unrelated to the MFGM, e.g., microbial quality. To resolve this, a modified approach to using lipase activity to probe MFGM was developed. Methanol (1%, w/w) and lipoprotein lipase (2%, w/w, raw milk) were added to pasteurised milk samples resulting in formation of methyl esters that therefore served as a new and unique signal for lipase gaining access to interfacial fat. Analysis conditions minimised product formation to minimise sample perturbation. The method could detect 1% (w/w) of homogenised milkfat globules blended with native globules, with linear response to 10% (w/w) and saturation above 20% (w/w). The approach was applied to assess MFGM in samples subjected to disruptive treatments (shear, aeration) under different conditions; findings were consistent with previous research, but with greater sensitivity.

Ghee, the clarified butter fat being the costliest fat among other edible oils and fats in India, prone to adulteration with highly manipulated cheaper oils/fats, especially during lean season. The present investigation carried on triglycerides profile of one of the latest components of the adulterant fat i.e. RM (Reichert–Meissl)-adjuster, has been exploited to check the adulteration of ghee with a newly emerged highly manipulated fat. Using standardized (S)-limits specified by the ISO/IDF for cow milk fat, the minimum level of detection of the adulterant fat was observed as 7.5%. However, in case of buffalo ghee, due to non-availability of the ISO/IDF limits, the detection of this adulterant fat in buffalo ghee was not possible. Gas chromatograms showed specific signature peaks of large size in the retention time region of 4.5 to 6.5 min for RM-adjuster and adulterant fat, whereas no such peaks were observed in pure cow and buffalo ghee samples. The new approach of zooming in and superimposing of selected peaks in the chromatograms of triglycerides of suspected ghee has been used as a strategy to find adulterant fat’s presence. Through this approach, the addition of RM-adjusted highly manipulated foreign fat (adulterant fat) to the tune of even 0.5% could be achieved in both cow as well as buffalo ghee.

Enzymatic reetherification of fats has numerous technological and economic advantages, which makes its large-scale implementation highly efficient. Unlike chemical modification, enzymatic reetherification demonstrates a greater specificity, typical of the catalytic action of lipase, and a higher controllability. Lipases with positional specificity cause redistribution of fatty acids to occur only in extreme provisions of triglycerides. In addition, this method is 1.5 times lower than hydrogenation of fats. The authors used the facilities of an innovative laboratory provided by JSC Eurasian Foods Corporation to conduct practical research on reetherification of fatty mixes. The main objective was to study the effect of the fats obtained by fermental reetherification on the quality indicators of butterfat substitutes. The research featured the input products to be used in the formula of reetherified fat and prepared fat mixes for butterfat substitutes. The paper describes the process of enzymatic reetherification of mixes of oils and fats, prepared reesterified fats, and buttermilk substitutes obtained from reetherified fats. The process involved a sequence of reactors filled with Lipozyme TL IM, a granulated substance of a microbic 1.3-specific lipase. The lipase was obtained from Thermomyces Lanuginosus, which had been immobilized with silica gel. The obtained products conformed to the butterfat standards in that they contained 16–2% of polynonsaturated fatty acids, no transisomers of fatty acids, ≤ 38% of palmitiny acid, and ≤ 5% of solid triglycerides at 35 of °C. The melting temperature was under body heat. The resulting characteristics of butterfat substitutes make them high-quality dairy products.

Long-term intake of high-fat food disrupts lipid metabolism, resulting in fat accumulation, obesity, and hyperlipidemia. Curcumin, active ingredients of turmeric plants, has been shown to possess potent antioxidant and hypolipidemic properties. The study was conducted to observe the effects of curcumin on butter induced hyperlipidemia, hematological values and physio-pathological alterations in liver and aorta in mice. A total of 40 Swiss Albino mice (Mus musculus), aged 28-30 days with an average body weight of 27g were randomly divided into 2 groups. Group A was considered as non-treated control (NT) (n=8) and fed on standard mice pellet and fresh drinking water. Another group was considered as butter group (n=32), fed on standard mice pellet enriched with 10% butter and after 5 weeks, mice of butter group were subdivided into 4 equal groups (n=8). Group B was considered as butter treated (BT) group fed on standard mice pellet enriched with 10% butter. Group C (C1%), group D (C2%) and group E (C3%) fed on standard mice pellet enriched with 10% butter plus curcumin 1%, 2% and 3% in drinking water respectively. The experiment was carried out for period of another 10 weeks. Body weight gain of all mice was gradually increased and maximum weight gain was recorded in mice of butter fed group. Supplementation of curcumin to butter treated mice (group C, D and E) prevented weight gain. Mice treated with butter fat had significantly (p

Objective The objective was to determine the DE of various lipids and compare these values with predicted values based on published equations. Materials and Methods Pigs (480) weaned at 24 d of age were fed a common diet for 7 d after which they were fed a basal or lipid-based diet for 28 d. Lipids (7.13%) were added in place of the basal diet, and all diets were pelleted and offered ad libitum. Fecal samples were collected on d 25 from 2 pigs per pen and pooled for subsequent analysis. Apparent total-tract digestibility of GE of the diet was determined by the indirect method and the DE content of each lipid source using the difference procedure. Results and Discussion The DE for butter fat, canola oil, coconut oil, fish oil, flaxseed oil, lard, olive oil, palm oil, soybean oil, and tallow was 8,911, 9,474, 9,380, 9,464, 8,584, 8,648, 9,606, 8,304, 9,979, and 8,071 kcal/kg, respectively. Using these values and the chemical composition of each lipid, the best fit prediction equation was DE, kcal/kg = 10,267 − (110.3 × FFA, %) − (41.8 × C16:0, %) − (39.7 × C18:0, %) − (98.0 × unsaturated:saturated ratio) + (6.4 × iodine value), SE = 164, R2 = 0.97, P = 0.01. Implications and Applications The determined DE herein were approximately 12% greater than predicted based on published equations, indicating the refining of published prediction equations is needed. These results indicate that DE of lipids can vary depending on their fatty acid composition, which consequently affects pig performance.

The adulteration of butter has become a major problem in food industries. Butter has the similar characteristic to lard which makes lard a desirable adulterant in butter due to economic advantages. Therefore, the method of detection to analyse the adulteration practice must be developed. This study used NMR spectroscopy in combination with chemometrics for the authentication of butter from lard. The presence of lard as an adulterant in butter has been analysed using Gas Chromatography–Mass Spectrometry (GC-MS) and Nuclear Magnetic Resonance (NMR) spectroscopy with the aid of chemometric of Principal Component Analysis (PCA) and Discriminant Analysis (DA). PC1 described 82% of the variation while PC2 accounted for 15% of the variation resulted in a model that described 80% of the total variance in the data. With 82% of the peak variation along the first PC, it was clear that all seventeen samples of butter in the market and pure lard sample were formed according to their own group and showed two well-defined and well-separated group. DA model classified 100% of all samples accurately according to its group (butter and butter adulterated with animal fats), meaning that no samples were misclassified into the wrong group. Lard was successfully determined at 2.63 ppm. In this study, NMR and PCA analysis has successfully discriminated between the market sample and lard and the results established that there is no lard being adulterated in all commercial butter samples. This could be a potential identification approach to determine if the product has been deceived in market.

Twelve samples of butter were purchased from the local markets and compared with control butter sample made in the lab.. The samples were kept under cooling till analysis. The samples were analyzed by gas chromatography for the fatty acids content, and also chemically analyzed for cholesterol levels and fat content. The chromatographic analysis revealed that only three samples were identical to the control sample, while the other samples varied from the control regarding fatty acid composition. The results revealed that five samples showed a marked decrease in the total short chain acids when compared to the control sample. It could also observed that four samples had higher content of lauric acid (C12), while three samples possessed high content of palmetic acid (C16:0). On the other hand,, two samples were characterized with higher levels of oleic acid (C18:1 ), and stearic acid (C18:0 ). These findings show that an adulteration with palm kerenal oil, palim oil and tallow, was done, respectively. The results obtained from chromatographic analysis enabled to detect the adulteration by using the fatty acids ratios between certain fatty acids. The ratio between C12/C10, C14/C12, C18:1/C14, C18:0/C18:2, C18:1/C18 and the total saturated fatty acids/total unsaturated fatty acids, were used to detect the adulteration. The ratios between C12/C10, C18:0/C18:2, C18:1/C18:0, and total saturated fatty acids /total unsaturated fatty acids were useful in detecting the adulteration of butter fat with vegetable oils or tallow. Cholesterol content of the samples was carried out. The results obtained revealed that the addition of adulterants to the butter decreased the cholesterol level of the adulterated samples when compared to the control sample. The decrease of the cholesterol level seems to be proportional to the adulteration ratios. Also, calculating the cholesterol level of the suspected samples regarding the cholesterol level of the control samples helped to detect the ratios of adulterants in butter samples. Fat content of all samples did not differ.

In Federal Milk Marketing Orders (FMMO), which use multiple component pricing schemas, farmers are paid for delivered quantity of butterfat, protein, and other solids, plus a producer price differential (PPD). The PPD captures the difference between the total handler obligations to the pool and the total component value of milk. In 2020, record negative PPD caused widespread frustration among dairy farmers. The primary objective of this research was to provide a comprehensive analysis of factors that affect PPD and to quantify their relative importance. We examined FMMO data from the past 10 yr including prices, utilizations, and tests. By decomposing the PPD, we were able to isolate the drivers of negative values. We found that long-term trends in usage, most importantly declining beverage milk and rising component tests, including both butterfat and protein, have substantially reduced PPD over the past decade. Class I milk pricing reform of 2018 exacerbated negative PPD in 2020, but we found that the PPD would have been negative even without that reform. The greatest contributor to recent negative PPD was the spread between cheese and milk powder prices, caused by shifts in demand due to the COVID-19 pandemic and USDA intervention cheese purchases. A range of policy modifications proposed to address negative PPD was evaluated.

The textural properties of butter are influenced by its fat content and implicitly by the fatty acids composition. The impact of butter’s chemical composition variation was studied in accordance with texture and color properties. From 37 fatty acids examined, only 18 were quantified in the analyzed butter fat samples, and approximately 69.120% were saturated, 25.482% were monounsaturated, and 5.301% were polyunsaturated. The butter samples’ viscosity ranged between 0.24 and 2.12 N, while the adhesiveness ranged between 0.286 to 18.19 N·mm. The principal component analysis (PCA) separated the butter samples based on texture parameters, fatty acids concentration, and fat content, which were in contrast with water content. Of the measured color parameters, the yellowness b* color parameter is a relevant indicator that differentiated the analyzed sample into seven statistical groups, the ANOVA statistics highlighted this difference at a level of p &lt, 0.001.

Brassica crops such as kale and swede can be supplied to cow diets during winter, however little is known about the effects of feeding those forage brassicas to lactating cows on cheese nutritional characteristics of milk and cheese. This study evaluated the effect of including kale or swede in pasture-fed lactating dairy cow diets on chemical composition, fatty acid (FA) profile, and sensory characteristics of Chanco-style cheese. Twelve early-lactation cows were used in a replicated (n = 4) 3 &times, 3 square Latin square design. The control diet consisted of (DM basis) 10.0 kg of grass silage, 4.0 kg of fresh grass pasture, 1.5 kg soybean meal, 1.0 kg of canola meal, and 4.0 kg of cereal-based concentrate. The other treatments replaced 25% of the diet with swede or kale. Milk yield, milkfat, and milk protein were similar between treatments as were cheese moisture, fat, and protein. Swede and kale increased total saturated cheese FA while thrombogenic index was greater in swede, but color homogeneity and salty flavor were greater while ripe cheese aroma less than for kale. Kale or swede can be used in the diet of pasture-fed lactating dairy cows without negative effects on milk production, milk composition, or cheese composition. However, kale and swede increased total cheese saturated FA.

The addition of plant oils such as soybean oil (S) to a diet rich in saturated fatty acids is discussed as a possible route to prevent or diminish the development of metabolic disease. Here, we assessed whether a butterfat-rich diet fortified with S affects the development of early non-alcoholic steatohepatitis (NASH) and glucose intolerance. Female C57BL/6J mice were fed a standard-control diet (C), a fat-, fructose-, and cholesterol-rich diet (FFC, 25E% butterfat, 50% (wt./wt.) fructose, 0.16% (wt./wt.) cholesterol), or FFC supplemented with S (FFC + S, 21E% butterfat + 4E% S) for 13 weeks. Indicators of liver damage, inflammation, intestinal barrier function, and glucose metabolism were measured. Lipopolysaccharide (LPS)-challenged J774A.1 cells were incubated with linolenic and linoleic acids (ratio 1:7.1, equivalent to S). The development of early NASH and glucose intolerance was significantly attenuated in FFC + S–fed mice compared to FFC-fed mice associated with lower hepatic toll-like receptor-4 mRNA expression, while markers of intestinal barrier function were significantly higher than in C-fed mice. Linolenic and linoleic acid significantly attenuated LPS-induced formation of reactive nitrogen species and interleukin-1 beta mRNA expression in J774A.1 cells. Our results indicate that fortifying butterfat with S may attenuate the development of NASH and glucose intolerance in mice.

Bulk tank milk samples from 392 Northern Ireland dairy farms and individual milk from animals (n = 293) on 4 of these farms were tested by a novel phagomagnetic separation (PhMS)-quantitative (q)PCR assay able to detect and quantify viable Mycobacterium avium ssp. paratuberculosis (MAP), to demonstrate its potential utility as a milk surveillance tool. Viable MAP were detected in 26.5% of the bulk tank milks, with MAP contamination levels ranging from 1 to 8,432 MAP/50 mL of milk; less than 2% of farms had MAP contamination levels >100 MAP/50 mL in their bulk tank milk. Follow-up PhMS-qPCR testing of milk from individual animals on 4 farms that had the highest numbers of MAP in their bulk tank milks indicated that 17 to 24% of animals in each herd were shedding viable MAP in their milk. Mean MAP numbers detected ranged between 6.7 and 42.1 MAP/50 mL of milk. No significant correlation was observed between the detection of viable MAP in bulk or individual milks by PhMS-qPCR and parallel milk ELISA results, or between PhMS-qPCR results and any other milk recording results (somatic cell count, total bacterial count, % butterfat, or % protein). Viable MAP was detected by IS900 qPCR in 52 (85.2%) Pozzato broth cultures of 61 PhMS-qPCR-positive individual milks after 12 wk of incubation, suggesting few PhMS-qPCR results were false positives. The mean sensitivities of the PhMS-qPCR assay and milk ELISA applied to individual milks were estimated by Bayesian latent class analysis to be 0.7096 and 0.2665, respectively, and mean specificities were similar (0.9626 and 0.9509). Our findings clearly demonstrate that the novel PhMS-qPCR assay could be a useful milk surveillance tool for dairy processors, or a milk monitoring tool for Johne's disease control or milk quality assurance programs.

There is increasing industrial interest in the use of the milkfat globule membrane as a food ingredient. The objective of this research was to determine whether the aerosol whipping performance of cream separated into butter and buttermilk, and then recombined, would perform in a manner similar to untreated cream. Churning of cream tempered to different solid fat contents was used to separate butter from buttermilk, which were then recombined at the same ratios as the initial extraction yield, or with 25% extra buttermilk. Differences in milkfat globule size distributions among the recombined creams were apparent; however, their whipping behavior and overrun were similar. Importantly, all recombined creams did not yield properties similar to the original cream, indicating that the unique native milkfat globule membrane structure plays a role in cream performance well beyond its simple presence.

This chapter focuses upon the sources of chemicals and toxins that commonly cause violative residues in livestock, and recommended procedures for decontamination of exposed animals. Suggestions are presented for the prevention of such residues through feeding and management techniques. Case histories of two actual incidents of livestock contamination are presented, together with the methods employed for decontamination of the herds. Chronic exposures may be more difficult to diagnose than acute ones. Appropriate samplings of blood, urine and feces, and biopsies of fat and other tissues may be indicated. Animals that have been contaminated by lipid soluble compounds, such as chlorinated pesticides and some industrial chemicals, may have violative residues in their body fat. Decontamination of the animal can be accelerated by promoting the loss of body fat through limited feeding, or increased butterfat production in milk, depending upon the management circumstances.