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9. Short-term safety outcomes of mastectomy and immediate implant-based breast reconstruction with and without mesh (iBRA): a multicentre, prospective cohort study

Author

Achuthan, R, Adwan, I, Aggarwal, S, Ahmed, M, Akelund, M, Akolekar, D, Al-Jibury, O, Amanita, M, Appleton, D, Archampong, D, Asgiersson, K, Athwal, R, Augusti, A, Ayaani, S, Bains, S, Baker, B, Baker, E, Baldota, S, Banerjee, D, Barker, S, Barr, L, Barry, P, Basu, N, Bathla, S, Bishop, N, Boland, G, Branford, O A, Bright-Thomas, R, Brindle, R, Brock, L, Brown, V, Bux, F, Byrne, G, Cain, H, Caldon, L, Callaghan, M, Carbone, A, Carpenter, R, Cawthorn, S, Chagla, L, Challoner, T, Chalmers, C, Chalmers, R, Chambers, S, Chana, M, Chand, N, Chandran, V, Chandrashekar, M, Charfare, H, Chatterjee, J, Chatterjee, S, Chattopadhyay, R, Chaudry, A, Chin, K, Chong, K, Chouhan, A, Choy, C, Christopoulos, P, Clarke, D, Clarke, S, Clayton, E, Clifford, R, Cocker, D, Collin, T, Collis, N, Conroy, F, Constantinou, C, Conway, A, Cook, J, Coombs, N, Cox, K, Critchley, A, Dakka, M, Dani, M, Daoud, R, Darragh, L, Darvesh, S, Dash, I, Datta, S, Davies, E, Dawson, S, De Sousa, E, Debnath, D, Deol, H, Devalia, H, Di Micco, R, Dicks, JR, Dickson, J, Dobner, N, Dobson, G, Dunne, N, Egbeare, D, El Sharief, D, Elfadl, D, Eltigani, E, Enver, D, Erel, E, Evans, A, Exarchos, G, Fage, E, Fatayer, H, Fenn, C, Ferguson, D, Foulkes, R, Franks, J, Fung, V, Galea, M, Gandamihardja, T, Gandhi, A, Garnsey, C, Gateley, C, Gattuso, J, Gawne, S, Geerthan, N, Ghattura, A, Giaramadze, A, Gill, J, Godden, AR, Goh, S, Govindarajulu, S, Goyal, S, Graja, T, Granger, S, Green, M, Grover, K, Gui, G, Gurung, R, Gutteridge, E, Hakim, A, Halka, A, Hamilton-Burke, W, Hamo, I, Harding-Mackean, C, Hargreaves, A, Harries, S, Harris, K, Harris, P, Harrison, S, Harvey, J, Hashem, M, Hassan, U, Henderson, J, Henton, J, Hignett, S, Hodgkins, K, Horgan, K, Horn, S, Hu, J, Hussain, A, Iddon, J, Iqbal, A, Irri, R, Irvine, T, Irwin, G, Iskender, A, Ismail, A, Ives, C, James, K, James, R, Jiwa, N, Jobson, M, Joglekar, S, Johnson, L, Johnson, R, Jones, L, Ju Hwang, M, Kalles, V, Kanesalingam, K, Karat, I, Kaushik, M, Kennedy, K, Khalifa, E, Khan, H, Khanbhai, M, Khawaja, S, Khout, H, Kiernan, T, Kim, B, Kirkpatrick, K, Kiruparan, P, Kirwan, C, Kishore, M, Kneeshaw, P, Knight, A, Kohlhardt, S, Krupa, J, Krupa, K, Kuruvilla, R, Laban, C, Lai, LM, Laidlaw, I, Lambert, K, Langlands, F, Lansdown, M, Laurence, N, Laws, S, Ledwidge, S, Lefemine, V, Lennon, H, Linforth, R, Little, K, Luangsomboon, A, Lund, J, Maalo, J, MacLennan, L, Macmillan, RD, MacNeil, F, Mahapatra, TK, Mallidis, E, Mallon, P, Manoloudakis, N, Maraqa, L, Marla, S, Masood, S, Massey, J, Masudi, T, Matey, P, Mazari, F, McCulley, S, McEvoy, K, Mcintosh, J, McIntosh, S, McKenzie, S, McManus, P, McNicholas, J, Michalakis, I, Mills, N, Mitchell, G, Monib, S, Mullan, M, Murphy, C, Murphy, G, Murphy, J, Murthy, B, Musa, S, Nagra, G, Nangalia, R, Narayanan, S, Nasr, R, Navin, C, Newton, R, Nicholson, S, Nuru, N, O'Connell, R, O'Donoghue, J, Ogedegbe, A, Olayinka, OS, Olsen, S, Osborn, G, Osborne, C, Osman, H, Otieno, C, Pakzad, F, Park, A, Parker, S, Partlett, P, Parvaiz, A, Parvanta, L, Patel, G, Peel, A, Peiris, L, Pennick, M, Peppe, A, Perry, D, Pilgrim, S, Piper, J, Poonawalla, S, Popa, E, Pope, V, Pugh, P, Rainsbury, D, Ramsey, K, Rasheed, T, Rathinaezhil, R, Rattay, T, Ravichandran, D, Reed, M, Refsum, S, Remoundos, D, Rigby, K, Robertson, S, Robinson, A, Robinson, J, Roche, N, Roy, PJ, Runkel, M, Rusby, J, Saha, S, Saidan, Z, Salab, M, Saleh, M, Salem, F, Sami, A, Samlalsingh, S, Sarfraz, N, Shah, R, Shaheed, S, Sharaiha, Y, Shetty, G, Shotton, R, Sircar, T, Skene, E, Sloan, S, Smith, B, Smith, J, Soldanova, L, Soliman, F, Soumian, S, Stevens, J, Steventon, C, Stewart-Parker, E, Stringfellow, T, Sutaria, R, Sutton, R, Sweetland, H, Swiech, B, Tadiparthi, S, Tafazal, H, Taheri, N, Tait, C, Tan, M, Tang, S, Tansley, A, Tate, S, Tayeh, S, Taylor, A, Taylor, J, Thawdar, P, Thomas, C, Thomas, S, Thomson, S, Thorne, A, Tillett, R, Tolkien, Z, Tomlins, A, Topps, A, Tsang, F, Turner, EJ, Turton, P, Udayasankar, S, Ugolini, F, Vaughan Williams, E, Vidya, R, Vijaynagar, B, Vinayagam, R, Volleamere, A, Voynov, V, Waheed, S, Walker, T, Walsh, U, Warner, R, Waters, R, Wilkins, A, Williams, K, Wilson, G, Wiltsher, M, Wooler, B, Wright, C, Wright, M, Wyld, L, Youssef, M, Zabkiewicz, C, Zammit, C, Zeidan, B, Zheng, D, Potter, Shelley, Conroy, Elizabeth J, Cutress, Ramsey I, Williamson, Paula R, Whisker, Lisa, Thrush, Steven, Skillman, Joanna, Barnes, Nicola L P, Mylvaganam, Senthurun, Teasdale, Elisabeth, Jain, Abhilash, Gardiner, Matthew D, Blazeby, Jane M, and Holcombe, Chris

Published
2019
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21. P229 IMPROVED CLINICAL–INSTRUMENTAL OUTCOMES IN PATIENTS WITH CHRONIC DILATED HYPERTENSIVE / ISCHEMIC HEART DISEASE, UNDERGOING CCM, CARRIER OF CRTD WITH PERSISTENT SEVERE LEFT VENTRICULAR DYSFUNCTION

Author

Incampo, G, primary, De Luca, V, additional, Rizzo, D, additional, Bux, F, additional, Moramarco, M, additional, Valecce, R, additional, Robles, G, additional, Pirozzi, M, additional, Candida, T, additional, Marino, F, additional, Campagna, M, additional, Fiorentino, A, additional, and Bonfantino, M, additional

Published
2022
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22. MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants

Author

Dueholm, M. K. D., Nierychlo, M., Andersen, K. S., Rudkjøbing, V., Knutsson, S., Arriaga, S., Bakke, R., Boon, N., Bux, F., Christensson, M., Chua, A. S. M., Curtis, T. P., Cytryn, E., Erijman, L., Etchebehere, C., Fatta-Kassinos, D., Frigon, D., Garcia-Chaves, M. C., Gu, A. Z., Horn, H., Jenkins, D., Kreuzinger, N., Kumari, S., Lanham, A., Law, Y., Leiknes, T. O., Morgenroth, E., Muszyński, A., Petrovski, S., Pijuan, M., Pillai, S. B., Reis, M. A. M., Rong, Q., Rossetti, S., Seviour, R., Tooker, N., Vainio, P., Loosdrecht, M. Van, Vikraman, R., Wanner, J., Weissbrodt, D., Wen, X., Zhang, T., Nielsen, P. H., Albertsen, M., and Consortium, MiDAS Global

Subjects
Chemical engineering, ddc:660
Abstract

Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’ database. MiDAS 4 is an amplicon sequence variant resolved, full-length 16S rRNA gene reference database with a comprehensive taxonomy from domain to species level for all sequences. We use an independent dataset (269 WWTPs) to show that MiDAS 4, compared to commonly used universal reference databases, provides a better coverage for WWTP bacteria and an improved rate of genus and species level classification. Taking advantage of MiDAS 4, we carry out an amplicon-based, global-scale microbial community profiling of activated sludge plants using two common sets of primers targeting regions of the 16S rRNA gene, revealing how environmental conditions and biogeography shape the activated sludge microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 966 genera and 1530 species that represent approximately 80% and 50% of the accumulated read abundance, respectively. Finally, we show that for well-studied functional guilds, such as nitrifiers or polyphosphate-accumulating organisms, the same genera are prevalent worldwide, with only a few abundant species in each genus.

Published
2022

23. MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants

Author

Dueholm, MKD, Nierychlo, M, Andersen, KS, Rudkjøbing, V, Knutsson, S, Arriaga, S, Bakke, R, Boon, N, Bux, F, Christensson, M, Chua, ASM, Curtis, TP, Cytryn, E, Erijman, L, Etchebehere, C, Fatta-Kassinos, D, Frigon, D, Garcia-Chaves, MC, Gu, AZ, Horn, H, Jenkins, D, Kreuzinger, N, Kumari, S, Lanham, A, Law, Y, Leiknes, TO, Morgenroth, E, Muszyński, A, Petrovski, Steve, Pijuan, M, Pillai, SB, Reis, MAM, Rong, Q, Rossetti, S, Seviour, Robert, Tooker, N, Vainio, P, van Loosdrecht, M, Vikraman, R, Wanner, J, Weissbrodt, D, Wen, X, Zhang, T, Nielsen, PH, and Albertsen, M

Subjects
FOS: Biological sciences, 90409 Wastewater Treatment Processes, Microbiology, FOS: Chemical engineering
Abstract

Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’ database. MiDAS 4 is an amplicon sequence variant resolved, full-length 16S rRNA gene reference database with a comprehensive taxonomy from domain to species level for all sequences. We use an independent dataset (269 WWTPs) to show that MiDAS 4, compared to commonly used universal reference databases, provides a better coverage for WWTP bacteria and an improved rate of genus and species level classification. Taking advantage of MiDAS 4, we carry out an amplicon-based, global-scale microbial community profiling of activated sludge plants using two common sets of primers targeting regions of the 16S rRNA gene, revealing how environmental conditions and biogeography shape the activated sludge microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 966 genera and 1530 species that represent approximately 80% and 50% of the accumulated read abundance, respectively. Finally, we show that for well-studied functional guilds, such as nitrifiers or polyphosphate-accumulating organisms, the same genera are prevalent worldwide, with only a few abundant species in each genus.

Published
2022
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36. Concentration and reduction of antibiotic residues in selected wastewater treatment plants and receiving waterbodies in Durban, South Africa

Author

Faleye, A. C., Adegoke, A. A., Ramluckan, K., Fick, Jerker, Bux, F., Stenstrom, T. A., Faleye, A. C., Adegoke, A. A., Ramluckan, K., Fick, Jerker, Bux, F., and Stenstrom, T. A.

Abstract

In the province of KwaZulu-Natal, South Africa the incidence of resistant tuberculosis, upper respiratory tract diseases as well as diarrhoeal and parasitic infections is high. Treatment of these diseases with antibiotics is partly reflected by the excretion of the respective antibiotics and their subsequent occurrence in wastewater. Their quantitative reduction in wastewater treatment reflects their potential environmental as well as human impact, the latter due to the use of the recipient water for domestic purposes and for irrigation. Information of the occurrence and reduction of different classes of antibiotics in wastewater treatment is sparse, especially the particle bound fraction of these. Due to this, analyses of aqueous and particle bound antibiotics in untreated wastewater of four selected wastewater treatment plants (WWTPs) and their receiving water bodies was carried out in Durban, South Africa. The treatment step especially considered was the biological one, represented by activated sludge and trickling filters. The treatment further included secondary clarifiers and final chlorine disinfection. Composite samples were collected during the period February 2017 to May 2017 and analysed with online solid phase extraction - high performance liquid chromatography mass spectrometry (SPE-HPLC-MS). For the 13 assessed antibiotics, the limit of detection (LOD) and the limit of quantification (LOQ) ranged from 0.07 to 0.33 ng L-1 and 0.23 to 1.09 ng L-1 respectively, while the total percentage recovery was in the range of 51 to 111%. The percentage of individual antibiotics bound to the particulate fraction normally lost by sample (influent) filtration, if not analysed in parallel, was in the range of 2.6%-97.3% (n = 32). In this fraction (sludge from centrifuge sample), the concentration of bound antibiotics of all the target antibiotics were detected in the influent of all WWTP in concentration ranges between 1.3 ng L-1 (Azithromycin; AZI) to 81,748 ng(-1) (C

Published
2019
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38. Short-term safety outcomes of mastectomy and immediate implant-based breast reconstruction with and without mesh (iBRA): a multicentre, prospective cohort study

Author

Potter, Shelley, primary, Conroy, Elizabeth J, additional, Cutress, Ramsey I, additional, Williamson, Paula R, additional, Whisker, Lisa, additional, Thrush, Steven, additional, Skillman, Joanna, additional, Barnes, Nicola L P, additional, Mylvaganam, Senthurun, additional, Teasdale, Elisabeth, additional, Jain, Abhilash, additional, Gardiner, Matthew D, additional, Blazeby, Jane M, additional, Holcombe, Chris, additional, Achuthan, R, additional, Adwan, I, additional, Aggarwal, S, additional, Ahmed, M, additional, Akelund, M, additional, Akolekar, D, additional, Al-Jibury, O, additional, Amanita, M, additional, Appleton, D, additional, Archampong, D, additional, Asgiersson, K, additional, Athwal, R, additional, Augusti, A, additional, Ayaani, S, additional, Bains, S, additional, Baker, B, additional, Baker, E, additional, Baldota, S, additional, Banerjee, D, additional, Barker, S, additional, Barr, L, additional, Barry, P, additional, Basu, N, additional, Bathla, S, additional, Bishop, N, additional, Boland, G, additional, Branford, O A, additional, Bright-Thomas, R, additional, Brindle, R, additional, Brock, L, additional, Brown, V, additional, Bux, F, additional, Byrne, G, additional, Cain, H, additional, Caldon, L, additional, Callaghan, M, additional, Carbone, A, additional, Carpenter, R, additional, Cawthorn, S, additional, Chagla, L, additional, Challoner, T, additional, Chalmers, C, additional, Chalmers, R, additional, Chambers, S, additional, Chana, M, additional, Chand, N, additional, Chandran, V, additional, Chandrashekar, M, additional, Charfare, H, additional, Chatterjee, J, additional, Chatterjee, S, additional, Chattopadhyay, R, additional, Chaudry, A, additional, Chin, K, additional, Chong, K, additional, Chouhan, A, additional, Choy, C, additional, Christopoulos, P, additional, Clarke, D, additional, Clarke, S, additional, Clayton, E, additional, Clifford, R, additional, Cocker, D, additional, Collin, T, additional, Collis, N, additional, Conroy, F, additional, Constantinou, C, additional, Conway, A, additional, Cook, J, additional, Coombs, N, additional, Cox, K, additional, Critchley, A, additional, Dakka, M, additional, Dani, M, additional, Daoud, R, additional, Darragh, L, additional, Darvesh, S, additional, Dash, I, additional, Datta, S, additional, Davies, E, additional, Dawson, S, additional, De Sousa, E, additional, Debnath, D, additional, Deol, H, additional, Devalia, H, additional, Di Micco, R, additional, Dicks, JR, additional, Dickson, J, additional, Dobner, N, additional, Dobson, G, additional, Dunne, N, additional, Egbeare, D, additional, El Sharief, D, additional, Elfadl, D, additional, Eltigani, E, additional, Enver, D, additional, Erel, E, additional, Evans, A, additional, Exarchos, G, additional, Fage, E, additional, Fatayer, H, additional, Fenn, C, additional, Ferguson, D, additional, Foulkes, R, additional, Franks, J, additional, Fung, V, additional, Galea, M, additional, Gandamihardja, T, additional, Gandhi, A, additional, Garnsey, C, additional, Gateley, C, additional, Gattuso, J, additional, Gawne, S, additional, Geerthan, N, additional, Ghattura, A, additional, Giaramadze, A, additional, Gill, J, additional, Godden, AR, additional, Goh, S, additional, Govindarajulu, S, additional, Goyal, S, additional, Graja, T, additional, Granger, S, additional, Green, M, additional, Grover, K, additional, Gui, G, additional, Gurung, R, additional, Gutteridge, E, additional, Hakim, A, additional, Halka, A, additional, Hamilton-Burke, W, additional, Hamo, I, additional, Harding-Mackean, C, additional, Hargreaves, A, additional, Harries, S, additional, Harris, K, additional, Harris, P, additional, Harrison, S, additional, Harvey, J, additional, Hashem, M, additional, Hassan, U, additional, Henderson, J, additional, Henton, J, additional, Hignett, S, additional, Hodgkins, K, additional, Horgan, K, additional, Horn, S, additional, Hu, J, additional, Hussain, A, additional, Iddon, J, additional, Iqbal, A, additional, Irri, R, additional, Irvine, T, additional, Irwin, G, additional, Iskender, A, additional, Ismail, A, additional, Ives, C, additional, James, K, additional, James, R, additional, Jiwa, N, additional, Jobson, M, additional, Joglekar, S, additional, Johnson, L, additional, Johnson, R, additional, Jones, L, additional, Ju Hwang, M, additional, Kalles, V, additional, Kanesalingam, K, additional, Karat, I, additional, Kaushik, M, additional, Kennedy, K, additional, Khalifa, E, additional, Khan, H, additional, Khanbhai, M, additional, Khawaja, S, additional, Khout, H, additional, Kiernan, T, additional, Kim, B, additional, Kirkpatrick, K, additional, Kiruparan, P, additional, Kirwan, C, additional, Kishore, M, additional, Kneeshaw, P, additional, Knight, A, additional, Kohlhardt, S, additional, Krupa, J, additional, Krupa, K, additional, Kuruvilla, R, additional, Laban, C, additional, Lai, LM, additional, Laidlaw, I, additional, Lambert, K, additional, Langlands, F, additional, Lansdown, M, additional, Laurence, N, additional, Laws, S, additional, Ledwidge, S, additional, Lefemine, V, additional, Lennon, H, additional, Linforth, R, additional, Little, K, additional, Luangsomboon, A, additional, Lund, J, additional, Maalo, J, additional, MacLennan, L, additional, Macmillan, RD, additional, MacNeil, F, additional, Mahapatra, TK, additional, Mallidis, E, additional, Mallon, P, additional, Manoloudakis, N, additional, Maraqa, L, additional, Marla, S, additional, Masood, S, additional, Massey, J, additional, Masudi, T, additional, Matey, P, additional, Mazari, F, additional, McCulley, S, additional, McEvoy, K, additional, Mcintosh, J, additional, McIntosh, S, additional, McKenzie, S, additional, McManus, P, additional, McNicholas, J, additional, Michalakis, I, additional, Mills, N, additional, Mitchell, G, additional, Monib, S, additional, Mullan, M, additional, Murphy, C, additional, Murphy, G, additional, Murphy, J, additional, Murthy, B, additional, Musa, S, additional, Nagra, G, additional, Nangalia, R, additional, Narayanan, S, additional, Nasr, R, additional, Navin, C, additional, Newton, R, additional, Nicholson, S, additional, Nuru, N, additional, O'Connell, R, additional, O'Donoghue, J, additional, Ogedegbe, A, additional, Olayinka, OS, additional, Olsen, S, additional, Osborn, G, additional, Osborne, C, additional, Osman, H, additional, Otieno, C, additional, Pakzad, F, additional, Park, A, additional, Parker, S, additional, Partlett, P, additional, Parvaiz, A, additional, Parvanta, L, additional, Patel, G, additional, Peel, A, additional, Peiris, L, additional, Pennick, M, additional, Peppe, A, additional, Perry, D, additional, Pilgrim, S, additional, Piper, J, additional, Poonawalla, S, additional, Popa, E, additional, Pope, V, additional, Pugh, P, additional, Rainsbury, D, additional, Ramsey, K, additional, Rasheed, T, additional, Rathinaezhil, R, additional, Rattay, T, additional, Ravichandran, D, additional, Reed, M, additional, Refsum, S, additional, Remoundos, D, additional, Rigby, K, additional, Robertson, S, additional, Robinson, A, additional, Robinson, J, additional, Roche, N, additional, Roy, PJ, additional, Runkel, M, additional, Rusby, J, additional, Saha, S, additional, Saidan, Z, additional, Salab, M, additional, Saleh, M, additional, Salem, F, additional, Sami, A, additional, Samlalsingh, S, additional, Sarfraz, N, additional, Shah, R, additional, Shaheed, S, additional, Sharaiha, Y, additional, Shetty, G, additional, Shotton, R, additional, Sircar, T, additional, Skene, E, additional, Sloan, S, additional, Smith, B, additional, Smith, J, additional, Soldanova, L, additional, Soliman, F, additional, Soumian, S, additional, Stevens, J, additional, Steventon, C, additional, Stewart-Parker, E, additional, Stringfellow, T, additional, Sutaria, R, additional, Sutton, R, additional, Sweetland, H, additional, Swiech, B, additional, Tadiparthi, S, additional, Tafazal, H, additional, Taheri, N, additional, Tait, C, additional, Tan, M, additional, Tang, S, additional, Tansley, A, additional, Tate, S, additional, Tayeh, S, additional, Taylor, A, additional, Taylor, J, additional, Thawdar, P, additional, Thomas, C, additional, Thomas, S, additional, Thomson, S, additional, Thorne, A, additional, Tillett, R, additional, Tolkien, Z, additional, Tomlins, A, additional, Topps, A, additional, Tsang, F, additional, Turner, EJ, additional, Turton, P, additional, Udayasankar, S, additional, Ugolini, F, additional, Vaughan Williams, E, additional, Vidya, R, additional, Vijaynagar, B, additional, Vinayagam, R, additional, Volleamere, A, additional, Voynov, V, additional, Waheed, S, additional, Walker, T, additional, Walsh, U, additional, Warner, R, additional, Waters, R, additional, Wilkins, A, additional, Williams, K, additional, Wilson, G, additional, Wiltsher, M, additional, Wooler, B, additional, Wright, C, additional, Wright, M, additional, Wyld, L, additional, Youssef, M, additional, Zabkiewicz, C, additional, Zammit, C, additional, Zeidan, B, additional, and Zheng, D, additional

Published
2019
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39. OVERCOMING EXTREME MEDICAL–SURGICAL EVENTS, IN CARRIERS OF CRTD AND CCM. CLINICAL STABILIZATION

Author

Incampo, G, Rizzo, D, Mallardi, A, Bux, F, Campagna, M, Ruggeri, R, Cuonzo, M, Valecce, R, Moramarco, M, Montemurro, M, Giannoccaro, V, Marino, F, Bonfantino, M, and Pirozzi, M

Abstract

A 63–year–old male patient suffering from chronic dilated ischemic heart disease, with severe depression of contractility and estimated EF of 16%, carrier of CRT–D and CCM, in follow up at our center. Extreme events of medical/surgical interest have occurred in the last period: the patient underwent strangulated hernia surgery with ileostomy and subsequent stay in intensive care. Transferred spontaneously to cardiology where he was treated for heart failure also with norepinephrine and levosimendan. Once the CCM was reprogrammed with sensing recalibration and optimal delivery of therapy for 7 hours a day, interspersed with regular breaks, the patient showed a gradual improvement in vital parameters and in general clinical conditions, with more adequate blood pressure and diuresis regimes. The presence of the double device (CRT–D and CCM), together with medical therapy, allowed the patient‘s stabilization and clinical remission, with discharge and transfer to a cardiovascular rehabilitation center. The Cardiac Rhythm Management guaranteed by the CRTD, with optimization of medical therapy, together with the positive inotropic action of cardiac contractility modulation (CCM), allowed the patient to overcome high intensity surgical and medical events.

Published
2024
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40. Comparative assessment of DNA extraction procedures for Ascaris spp. eggs.

Author

Amoah, I.D., Singh, G., Troell, K., Reddy, P., Stenström, T.A., and Bux, F.

Subjects
DNA, ASCARIS, ASCARIS suum, SEWAGE sludge, POLYMERASE chain reaction, EGGS
Abstract

A central and critical step in the molecular detection of soil-transmitted helminths from environmental sources is the extraction of DNA from the eggs. In this study, we investigated the yield of DNA extracted from known quantities (500, 100, 50, 20, 10 and 5) of Ascaris suum eggs, as well as directly from wastewater and sludge samples containing Ascaris spp. eggs, using six commercial DNA extraction kits. The amount of DNA extracted was quantified with NanoDrop, Qubit and Ct values from quantitative polymerase chain reaction (qPCR) assay using CFX96 Touch™ real-time PCR equipment. The PowerLyzer Ultraclean Microbial DNA isolation kit and PowerSoil DNA isolation kit gave the highest yield of DNA based on the NanoDrop, Qubit and Ct values. However, the qPCR results indicate that in some of the kits, PCR inhibitors may have been carried over to the PCR reaction. DNA extraction kits that incorporate a bead-beating step as well as other mechanical eggshell disruption steps were superior in extracting DNA from Ascaris spp. eggs. Additionally, for the accurate quantification of extracted DNA, the use of Ct values from qPCR and Qubit readings gives better results compared to the NanoDrop readings. For efficient downstream applications, the use of DNA extraction kits with superior inhibitor removal technology is essential, in addition to a high yield of DNA. [ABSTRACT FROM AUTHOR]

Published
2020
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