Search

Your search keyword '"Céline BOBY"' showing total 31 results
Start Over Author "Céline BOBY"
31 results on '"Céline BOBY"'

1. Transcriptome profiling reveals stress-responsive gene networks in cattle muscles

Author

Isabelle Cassar-Malek, Lise Pomiès, Anne de la Foye, Jérémy Tournayre, Céline Boby, and Jean-François Hocquette

Subjects
Pre slaugther stress, Cattle, Transcriptomics, Muscle response, Transcription factors, Medicine, Biology (General), QH301-705.5
Abstract

In meat-producing animals, preslaughter operations (e.g., transportation, mixing unfamiliar animals, food and water deprivation) may be a source of stress with detrimental effects on meat quality. The objective of this work was to study the effect of emotional and physical stress by comparing the transcriptomes of two muscles (M. longissimus thoracis, LT and M. semitendinosus, ST) in Normand cows exposed to stress (n = 16) vs. cows handled with limited stress (n = 16). Using a microarray, we showed that exposure to stress resulted in differentially expressed genes (DEGs) in both muscles (62 DEGs in LT and 32 DEGs in ST, of which eight were common transcription factors (TFs)). Promoter analysis of the DEGs showed that 25 cis transcriptional modules were overrepresented, of which nine were detected in both muscles. Molecular interaction networks of the DEGs targeted by the most represented cis modules helped identify common regulators and common targets involved in the response to stress. They provided elements showing that the transcriptional response to stress is likely to (i) be controlled by regulators of energy metabolism, factors involved in the response to hypoxia, and inflammatory cytokines; and (ii) initiate metabolic processes, angiogenesis, corticosteroid response, immune system processes, and satellite cell activation/quiescence. The results of this study demonstrate that exposure to stress induced a core response to stress in both muscles, including changes in the expression of TFs. These factors could relay the physiological adaptive response of cattle muscles to cope with emotional and physical stress. The study provides information to further understand the consequences of these molecular processes on meat quality and find strategies to attenuate them.

Published
2022
Full Text
View/download PDF

2. Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle foetuses

Author

Isabelle Cassar-Malek, Céline Boby, Brigitte Picard, Antonio Reverter, and Nicholas J. Hudson

Subjects
Skeletal muscle, Myostatin, Mitochondria, Genomics, Myogenesis, Science, Biology (General), QH301-705.5
Abstract

The Blonde d'Aquitaine (BA) is a French cattle breed with enhanced muscularity, partly attributable to a MSTN mutation. The BA m. Semitendinosus has a faster muscle fibre isoform phenotype comprising a higher proportion of fast type IIX fibres compared to age-matched Charolais (CH). To better understand the molecular network of modifications in BA compared to CH muscle, we assayed the transcriptomes of the m. Semitendinosus at 110, 180, 210 and 260 days postconception (dpc). We used a combination of differential expression (DE) and regulatory impact factors (RIF) to compare and contrast muscle gene expression between the breeds. Prominently developmentally regulated genes in both breeds reflected the replacement of embryonic myosin isoforms (MYL4, MYH3) with adult isoforms (MYH1) and the upregulation of mitochondrial metabolism (CKMT2, AGXT2L1) in preparation for birth. However, the transition to a fast, glycolytic muscle phenotype in the MSTN mutant BA is detectable through downregulation of various slow twitch subunits (TNNC1, MYH7, TPM3, CSRP3) beyond 210 dpc, and a small but consistent genome-wide reduction in mRNA encoding the mitoproteome. Across the breeds, NRIP2 is the regulatory gene possessing a network change most similar to that of MSTN.

Published
2017
Full Text
View/download PDF

3. Adipocyte/breast cancer cell crosstalk in obesity interferes with the anti-proliferative efficacy of tamoxifen.

Author

Lauriane Bougaret, Laetitia Delort, Hermine Billard, Camille Le Huede, Céline Boby, Anne De la Foye, Adrien Rossary, Ali Mojallal, Odile Damour, Céline Auxenfans, Marie Paule Vasson, and Florence Caldefie-Chezet

Subjects
Medicine, Science
Abstract

Obesity is a well-known risk factor of breast cancer in post-menopausal women that also correlates with a diminished therapeutic response. The influence of adipocytes and their secretome, i.e. adipokines, on the efficacy of hormone therapy has yet to be elucidated.We investigated, ex vivo, whether mature adipocytes, differentiated from adipose stem cells of normal-weight (MA20) or obese (MA30) women, and their secretions, were able to counteract the effects of tamoxifen (Tx) which is known to decrease neoplastic cell proliferation.In a tridimensional model and in a model of co-culture, the anti-proliferative effect of Tx on MCF-7 cancer cells was counteracted by MA30. These two models highlighted two different specific gene expression profiles for genes encoding cytokines or involved in angiogenesis based on the adipocyte microenvironment and the treatment. Thus it notably showed altered expression of genes such as TNFα that correlated with IL-6. In addition, leptin, IL-6 and TNFα, at concentrations reflecting plasma concentrations in obese patients, decreased the anti-proliferative efficacy of 4-hydroxytamoxifen (a major active metabolite of Tx).These findings bring insights on adipocytes and mammary cancer cell interactions in Tx therapy, particularly in overweight/obese people. Indeed, patient' adipokine status would give valuable information for developing individual strategies and avoid resistance to treatment.

Published
2018
Full Text
View/download PDF

4. Dietary flavanols modulate the transcription of genes associated with cardiovascular pathology without changes in their DNA methylation state.

Author

Dragan Milenkovic, Wim Vanden Berghe, Céline Boby, Christine Leroux, Ken Declerck, Katarzyna Szarc vel Szic, Karen Heyninck, Kris Laukens, Martin Bizet, Matthieu Defrance, Sarah Dedeurwaerder, Emilie Calonne, Francois Fuks, Guy Haegeman, Guido R M M Haenen, Aalt Bast, and Antje R Weseler

Subjects
Medicine, Science
Abstract

BackgroundIn a recent intervention study, the daily supplementation with 200 mg monomeric and oligomeric flavanols (MOF) from grape seeds for 8 weeks revealed a vascular health benefit in male smokers. The objective of the present study was to determine the impact of MOF consumption on the gene expression profile of leukocytes and to assess changes in DNA methylation.Methodology/principal findingsGene expression profiles were determined using whole genome microarrays (Agilent) and DNA methylation was assessed using HumanMethylation450 BeadChips (Illumina). MOF significantly modulated the expression of 864 genes. The majority of the affected genes are involved in chemotaxis, cell adhesion, cell infiltration or cytoskeleton organisation, suggesting lower immune cell adhesion to endothelial cells. This was corroborated by in vitro experiments showing that MOF exposure of monocytes attenuates their adhesion to TNF-α-stimulated endothelial cells. Nuclear factor kappa B (NF-κB) reporter gene assays confirmed that MOF decrease the activity of NF-κB. Strong inter-individual variability in the leukocytes' DNA methylation was observed. As a consequence, on group level, changes due to MOF supplementation could not be found.ConclusionOur study revealed that an 8 week daily supplementation with 200 mg MOF modulates the expression of genes associated with cardiovascular disease pathways without major changes of their DNA methylation state. However, strong inter-individual variation in leukocyte DNA methylation may obscure the subtle epigenetic response to dietary flavanols. Despite the lack of significant changes in DNA methylation, the modulation of gene expression appears to contribute to the observed vascular health effect of MOF in humans.

Published
2014
Full Text
View/download PDF

6. Effects of the apple matrix on the postprandial bioavailability of flavan-3-ols and nutrigenomic response of apple polyphenols in minipigs challenged with a high fat meal

Author

Christine Morand, Geoffrey Istas, Claire Dufour, Didier Rémond, Dominique Bayle, Caroline Buffière, Sylvie Mercier, Dragan Milenkovic, Laurent-Emmanuel Monfoulet, Céline Boby, Ana Rodriguez-Mateos, Carine Le Bourvellec, Patrick Borel, Monfoulet, Laurent-Emmanuel, Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), King‘s College London, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Nutrition & Dietetics, King's College London's Faculty of Life Sciences & Medicine, Institut National de la Recherche Agronomique (INRA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and University of Reading (UOR)

Subjects
Male, Swine, 030309 nutrition & dietetics, Biological Availability, Diet, High-Fat, Peripheral blood mononuclear cell, Random Allocation, 03 medical and health sciences, chemistry.chemical_compound, Nutrigenomics, Flavan, Gene expression, Animals, Food science, 030304 developmental biology, Flavonoids, 2. Zero hunger, 0303 health sciences, Meal, Microarray analysis techniques, Polyphenols, General Medicine, Postprandial Period, Bioavailability, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Postprandial, chemistry, Polyphenol, Malus, bacteria, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract

International audience; Food matrix interactions with polyphenols can affect their bioavailability and as a consequence may modulate their biological effects. The aim of this study was to determine if the matrix and its processing would modulate the bioavailability and the postprandial nutrigenomic response to a dietary inflammatory stress of apple flavan-3-ol monomers. We carried out an acute randomized controlled study in minipigs challenged with a high fat meal (HFM) supplemented with raw fruit, puree, or apple phenolic extract with matched content of flavan-3-ol monomers. Fasting and postprandial blood samples were collected over 3 h to quantify flavan-3-ol monomers in sera by UPLC-Q-TOF/MS and to isolate peripheral blood mononuclear cells (PBMCs) for assessing the changes in the gene expression profile using a microarray analysis. When compared to the extract-supplemented meal, the peak of the total flavan-3-ol concentration was reduced by half with both raw apple and puree supplements. The apple matrices also affected the gene expression profile as revealed by the Principal Component Analysis of the microarray data from PBMCs which discriminated the supplementation of HFM with the polyphenol extract from those with raw apples or puree. A total of 309 genes were identified as differentially expressed by the apple-derived products compared to HFM, with 63% modulated only in the presence of the food matrix (apple and puree). The number of differentially modulated genes was higher with the puree (246) than with the unprocessed apple (182). Pathway enrichment analyses revealed that genes affected by the apple-derived products control inflammation and leukocyte transendothelial migration both involved in the onset of atherosclerotic processes. Overall, this study showed that the two apple matrices reduce the postprandial serum concentration of flavon-3-ols whereas they increase the nutrigenomic response of PBMCs. The biological processes identified as modulated by the apple products suggest an attenuation of the transient proinflammatory response induced by a HFM. The differences observed between the nutrigenomic responses support that the apple matrix and its processing affect the nutrigenomic response, probably by increasing the bioavailability of other apple phytochemicals. To conclude, this study raises awareness for considering the impact of the food matrix and its processing on the biological response of polyphenols in nutritional studies.

Published
2020
Full Text
View/download PDF

7. Talkmine, a workflow for the prediction of the interactions between secretome and surfaceome in the dialogue between cellular types

Author

Manon Connault, Jérémy Tournayre, Céline BOBY, Muriel Bonnet, Nadia Goué, Plateforme Auvergne Bioinformatique (AuBi), Mésocentre Clermont Auvergne, Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BOBY, Céline, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
[SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

8. Comparative transcriptome analysis of goat (Capra hircus) adipose tissue reveals physiological regulation of body reserve recovery after the peak of lactation

Author

Yannick Faulconnier, Céline Boby, Fanny Coulpier, Sophie Lemoine, Patrice Martin, and Christine Leroux

Subjects
Mammary Glands, Animal, Adipose Tissue, Physiology, Gene Expression Profiling, Goats, Genetics, Animals, Lactation, Female, Transcriptome, Lipids, Molecular Biology, Biochemistry
Abstract

Adipose tissue is the energy storage organ providing energy to other tissues, including mammary gland, that supports the achievement of successive lactation cycles. Our objective was to investigate the ability of goats to restore body fat reserves by comparing lipogenic enzyme activities and by transcriptomic RNA-Seq data at two different physiological stages, mid- and post-lactation. Key lipogenic enzyme activities were higher in goat omental adipose tissue during mid-lactation (74 days in milk) than during the post-lactation period (300 days postpartum). RNA-Sequencing analysis revealed 19,271 expressed genes in the omental adipose tissue. The comparison between adipose transcriptome analysis from mid- and post-lactation goats highlighted 252 differentially expressed genes (p

Published
2022
Full Text
View/download PDF

9. Different miRNA contents between mammary epithelial cells and milk fat globules: a random or a targeted process?

Author

Nicolas Crapart, Christine Leroux, Gleidson Giordano Pinto de Carvalho, Claudia Bevilacqua, Céline Boby, Patrice Martin, Karol Pawlowski, Lenha Mobuchon, Yannick Faulconnier, Daiane Lago-Novais, Warsaw University of Life Sciences (SGGW), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universidade Federal da Bahia (UFBA), Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Excilone [Elancourt], PHASE (Physiologie Animale & Systemes d'Elevage) Department of INRA Brazilian Ministry of Science and Technology 'Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior' (CAPES) GISA meta-program of INRA (Ruminflame project), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), Warsaw University of Life Sciences, and AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0301 basic medicine, Cell type, Mammary gland, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Lactating cow, Biology, Extracellular vesicles, 03 medical and health sciences, Mammary Glands, Animal, 0302 clinical medicine, microRNA, Genetics, medicine, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Glycoproteins, Milk fat, Mammary tissue, Epithelial Cells, MicroRNA, Lipid Droplets, General Medicine, Cell biology, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Nucleic acid, Cattle, Female, Glycolipids, Mammary epithelial cells
Abstract

International audience; MicroRNAs (miRNAs) are small noncoding RNAs present in milk-derived extracellular vesicles and milk fat globules(MFG). Nucleic acid content between the lactating mammary tissue (MT) and MFG are quite similar but discrepanciesexist in their miRNA content. Our objective was to identify the origin of these discrepancies, and to evaluate the existenceof a possible mechanism sorting miRNAs that will or will not be exported from the mammary epithelial cells (MECs) inbovine MFG. miR-125b-5p, miR-126-3p, miR-141-3p, and miR-204-5p, chosen on the basis of their abundance in the MT,were quantified using RT-qPCR in lactating cow MT, MFG, and laser capture-microdissected MECs. Two miRNAs (miR-125b-5p and miR-141-3p) were detected in the MT as well as in MFG and MECs. miR-204-5p was detected only in the MT,suggesting that it is very likely expressed in a cell type other than MECs. miR-126-3p was detected both in the MT and inMECs but not in MFG, suggesting a targeting mechanism for miRNAs in MECs. These results highlights differences inmiRNA content between MECs and MFG may be due to a possibly not random mechanism for loading MFG with miRNAcargos that could involve a variable distribution in MECs or a sorting mechanism.

Published
2020
Full Text
View/download PDF

10. Effect of apple food matrix on plasma flavn-3-ols distribution and nutrigenomic profile in response to a nutritional challenge in minipigs

Author

Dragan Milenkovic, Céline Boby, Sylvie Mercier, Claire Dufour, Christine Morand, Geoffrey Istas, Caroline Buffière, Dominique Bayle, Carine le Bourvelec, Ana Rodriguez-Mateos, Didier Rémond, Laurent-Emmanuel Monfoulet, Patrick Borel, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Department of Nutrition & Dietetics, King's College London's Faculty of Life Sciences & Medicine, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
2. Zero hunger, 0303 health sciences, Nutrition and Dietetics, Distribution (number theory), 030309 nutrition & dietetics, Chemistry, [SDV]Life Sciences [q-bio], Medicine (miscellaneous), 030209 endocrinology & metabolism, Plasma, 03 medical and health sciences, Matrix (mathematics), chemistry.chemical_compound, 0302 clinical medicine, Flavan, Food science, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

Food matrix is known to interact with some dietary constituents and microconstituents during digestion. These interactions may potentially affect the metabolism and bioavailability of some compounds, and as a consequence modulate their biological effects. In this context, the aim of this study was to determine the effect of apple food matrix on the bioavailability of flavan-3-ols and on the ability of these compounds to modulate the nutrigenomic response to a high fat challenge in minipigs.Adult male Yucatan minipigs (n = 5) were assigned to a random treatment sequence of high-fat meals non supplemented or supplemented with 250 g of raw apple, 250 g of apple puree or 1.4 g of apple polyphenols extract, with a 7-days washout period between each treatment. Each supplementation provided 155 mg flavan-3-ol monomers. At each treatment period, fasting- and 1h-, 2h-, 3h-postprandial blood samples were collected, and the concentration in flavan-3-ol monomers was measured on hydrolyzed serum, using UPLC-Q-TOF MS. The ability of apple-derived products to modulate the postprandial gene expression profile was assessed and compared in circulating PBMCs collected at 3 h after consumption of the four tested meals using a microarray analysis.Results show that the apple matrix did not affect the kinetic of the postprandial absorption of flavan-3-ol monomers. The total flavan-3-ols concentrations measured at peak were significantly higher in the extract (x1.75), suggesting an impact of the apple matrix on flavan-3-ols absorption. However, no significant difference in total flavanols was observed between raw apple and apple puree.Principal Component Analysis of the microarray data from PBMCs identified three distinct clusters of gene expression patterns: one corresponding to gene expression profiles after the high-fat meal, one for meal supplemented with raw apples or apple puree, and a third cluster for meal supplemented with polyphenol extract. A set of 309 genes was identified as differentially expressed by apple-derived products compared to high-fat meal alone, including 93 modulated with the three apple products. The variations in gene expression were similar for only 75% of the 93 genes, suggesting that the apple matrix affects the nutrigenomic response to flavan-3-ols. A bioinformatics analysis revealed that genes affected by apple-derived products are involved in inflammation and leukocyte transendothelial migration, suggesting a beneficial impact of apple-derived products.In conclusion, these results raise awareness for considering the impact of food matrix on the biological responsiveness of polyphenols in future nutritional studies.

Published
2019

11. La comparaison des profils miARN des globules gras du lait et des cellules épithéliales mammaires suggèrent une distribution différente selon les compartiments cellulaires

Author

Christine Leroux, Karol Pawlowski, Daiane Lago-Novais, Sébastien Bes, Claudia Bevilacqua, Yannick Faulconnier, Lenha Mobuchon, Nicolas Crapart, José Pires, Céline BOBY, Carvalho, G., Martin, P., Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade Federal da Bahia (UFBA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Excilone [Elancourt], and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, cellules épithéliales mammaires, [SDV]Life Sciences [q-bio], Tri des miARNs, glande mammaire, globules gras, lait, cellule épithéliale mammaire, globule lipidique, [INFO]Computer Science [cs], [SHS]Humanities and Social Sciences
Abstract

National audience; Les microARN (miARNs) sont de petits ARNs non codants régulant l'expression génique au niveau posttranscriptionnel. Ils sont synthétisés par les cellules et sont aussi présents dans les fluides physiologiques tels que le lait, dans les vésicules extracellulaires ainsi que dans les globules gras qui sont sécrétés en grande quantité dans le lait. Si l’on considère que les miARNs contenus dans les globules gras du lait sont majoritairement le reflet de ceux du tissu mammaire, le profil de certains diffère. Ceci pose la question de l’origine des miARNs présents dans les globules gras ainsi que de leur mode de sécrétion. L'objectif de la présente étude pilote était d'évaluer l'existence d'un possible mécanisme de tri des miARNs produits par la cellule épithéliale mammaire (CEM) et de leur export ou non via les globules gras du lait. Pour cela, 12 vaches en lactation ont permis la collecte de glande mammaire (n=6) et de lait (n=6). Trois préparations de CEM, capturées par microdissection laser, ont été effectuées. Quatre miARNs (miR-125b-5p, miR-126-3p, miR-141-3p et miR-204-5p) ont été choisis sur la base de leur abondance différente dans le lait et dans le tissu mammaire. Après extraction des ARN totaux (kit miRVana), les miARNs ont été quantifiés par RTqPCR (kits TaqMan® MicroRNA Reverse Transcription et TaqMan® Advanced miRNA cDNA synthesis + TaqMan® Fast Advanced Master Mix) dans le tissu mammaire (parenchyme sécrétoire profond), dans la matière grasse du lait, et les CEM microdisséquées. Deux miARNs (miR-125b-5p et miR-141-3p) ont été détectés dans le tissu mammaire, la matière grasse et les CEM, tandis que miR-204-5p n'a été détecté que dans le tissu mammaire. Cela suggère que ce miARN pourrait être exprimé dans un type cellulaire mammaire autre que les CEM, par exemple les cellules myoépithéliales, immunitaires ou les adipocytes mammaires. Le résultat le plus intéressant concerne miR-126-3p qui a été détecté dans le tissu mammaire, les CEM, mais pas dans la matière grasse du lait, ce qui suggère une localisation différente ou un possible mécanisme d'adressage des miARNs vers les voies de sécrétion ou non dans les CEM. En conclusion, cette étude pilote met en évidence des différences qualitatives de contenu en miARNs entre les CEM et la matière grasse laitière. Ces résultats laissent penser qu’il existerait un mécanisme non aléatoire de chargement des miARNs dans les globules gras, à l'image des processus de tri décrits pour les vésicules extracellulaires. Ce mécanisme reste à élucider pour ce qui concerne les globules gras.

Published
2019

12. Circulating anthocyanin metabolites mediate vascular benefits of blueberries: insights from randomized controlled trials, metabolomics, and nutrigenomics

Author

Geoffrey Istas, Ana Rodriguez-Mateos, Christian Heiss, Charlotte E. Mills, Sergio Gómez-Alonso, Dragan Milenkovic, Céline Boby, Rodrigo P. Feliciano, Lisa Boschek, Division of Cardiology, Pulmonology, and Vascular Medicine, University Hospital Düsseldorf, Department of Food and Nutritional Sciences, University College Cork (UCC), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Instituto Regional de Investigación Científica Aplicada (IRICA), Universidad de Castilla-La Mancha (UCLM), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Department of Internal Medicine, Division of Cardiovascular Medicine, University of California [Davis] (UC Davis), University of California-University of California, University of Surrey (UNIS), Surrey and Sussex Healthcare NHS Trust, Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM), and University of California (UC)-University of California (UC)

Subjects
0301 basic medicine, Aging, Endothelium, Cellular differentiation, Blueberry Plants, Omics, 030204 cardiovascular system & hematology, Pharmacology, Peripheral blood mononuclear cell, Anthocyanins, 03 medical and health sciences, chemistry.chemical_compound, Nutrigenomics, 0302 clinical medicine, Immune system, Metabolomics, endothelial function, medicine, Animals, Humans, polyphenols, Randomized Controlled Trials as Topic, 2. Zero hunger, blueberries, business.industry, Polyphenols, Endothelial function, Blueberries, metabolomics, 3. Good health, omics, 030104 developmental biology, medicine.anatomical_structure, nutrition, chemistry, Cardiovascular Diseases, Anthocyanin, Models, Animal, Endothelium, Vascular, Geriatrics and Gerontology, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Phytotherapy
Abstract

Potential health benefits of blueberries may be due to vascular effects of anthocyanins that predominantly circulate in blood as phenolic acid metabolites. We investigated which role blueberry anthocyanins and circulating metabolites play in mediating improvements in vascular function and explore potential mechanisms using metabolomics and nutrigenomics. Purified anthocyanins exerted a dose-dependent improvement of endothelial function in healthy humans, as measured by flow-mediated dilation. The effects were similar to those of wild blueberries containing similar amounts of anthocyanins, whereas control drinks containing fiber, minerals, or vitamins had no significant effect. Daily 1-month wild blueberry consumption increased flow-mediated dilation and lowered 24-hour ambulatory systolic blood pressure. Of the 63 anthocyanin plasma metabolites quantified, 14 and 21 correlated with acute and chronic flow-mediated dilation improvements, respectively. Injection of these metabolites improved flow-mediated dilation in mice. Daily wild blueberry consumption led to differential expression (>1.2-fold) of 608 genes and 3 microRNAs, with Mir-181c showing a 13-fold increase in peripheral blood mononuclear cells. Patterns of 13 metabolites were independent predictors of gene expression changes and pathway enrichment analysis revealed significantly modulated biological processes involved in cell adhesion, migration, immune response, and cell differentiation. Our results identify anthocyanin metabolites as major mediators of vascular bioactivities of blueberries and changes of cellular gene programs. Trial registration: NCT025208.

Published
2019
Full Text
View/download PDF

13. Effects of Azgp1

Author

Yannick, Faulconnier, Céline, Boby, José, Pires, Cyril, Labonne, and Christine, Leroux

Subjects
Blood Glucose, Mice, Knockout, Body Weight, Fatty Acids, Gene Expression, Lipid Metabolism, Lipids, Mammary Glands, Animal, Milk, Adipokines, Adipose Tissue, Liver, Animals, Lactation, Female, Carrier Proteins, Glycoproteins
Abstract

The expression of Azgp1 gene, an adipokine involved in the mobilization of body reserves, was observed in mammary gland of ruminants. Its regulation by different dietary conditions suggests a potential role in the mechanisms controlling the composition of milk fat. The aim of this study was to evaluate the role of Azgp1 during lactation. Azgp1

Published
2018

14. Extruded linseed alone or in combination with fish oil modifies mammary gene expression profiles in lactating goats

Author

Christine Leroux, Céline Boby, Laurence Bernard, Jordann Domagalski, Yves Chilliard, Yannick Faulconnier, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
0301 basic medicine, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, medicine.medical_specialty, Peroxisome proliferator-activated receptor gamma, mammary gland, Protein metabolism, Biology, SF1-1100, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, Fish Oils, Mammary Glands, Animal, nutrigenomics, Flax, Internal medicine, Gene expression, medicine, caprine, Animals, Lactation, lipid supplementation, 2. Zero hunger, chemistry.chemical_classification, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Goats, Fatty Acids, 0402 animal and dairy science, Fatty acid, Lipid metabolism, AZGP1, 04 agricultural and veterinary sciences, Fish oil, 040201 dairy & animal science, Animal culture, Diet, 030104 developmental biology, Endocrinology, Gene Expression Regulation, chemistry, Cattle, Female, Animal Science and Zoology, transcriptome
Abstract

International audience; Nutrition is a major factor that regulates ruminant milk components, particularly its fatty acid (FA) composition, which is an important determinant of milk nutritional quality. In the mammary gland, milk component biosynthesis involves a large number of genes under nutritional regulation that are not well understood. Thus, the objective of the present study was to evaluate the effects of extruded linseeds (EL) alone or in combination with fish oil (ELFO) on goat mammary gene expression. In total, 14 goats were fed one of the following three diets: a natural grassland hay basal diet (CTRL) alone, CTRL supplemented with 530 g/day of EL, or 340 g/day of EL plus 39 g/day of fish oil (ELFO). Mammary secretory tissues were collected after slaughter on day 28, to determine the expression of 14 lipogenic genes and five lipogenic enzyme activities and transcriptomic profiles. The mRNA abundance decreased for SCD1 ( P

Published
2018
Full Text
View/download PDF

15. Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability

Author

Laurent-Emmanuel Monfoulet, Dragan Milenkovic, Céline Boby, Irene Krga, Sylvie Mercier, Radu Tamaian, Christine Morand, Marija Glibetić, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Centre of Research Excellence in Nutrition and Metabolism, University of Belgrade [Belgrade], R&D, National Institute of Research and Development for Cryogenic and Isotopic Technologies ICSI Rm. Valcea, Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Department of Internal Medicine, Division of Cardiovascular Mededicine, University of California [Davis] (UC Davis), University of California-University of California, University of California, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), University of California (UC)-University of California (UC), University of California (UC), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
0301 basic medicine, Cell signaling, fonction endothéliale, Gut metabolites, Biochemistry, Monocytes, MiRNA expression, mécanisme moléculaire, Capillary Permeability, Focal adhesion, Anthocyanins, 03 medical and health sciences, Nutrigenomics, Western blot, anthocyanine, Physiology (medical), Gene expression, Cell Adhesion, Human Umbilical Vein Endothelial Cells, medicine, Humans, Mode of action, Transcription factor, Transendothelial migration, medicine.diagnostic_test, Monocyte adhesion, Chemistry, Microarray analysis techniques, fungi, Transendothelial and Transepithelial Migration, food and beverages, Endothelial function, approche omique, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Cell biology, Endothelial stem cell, carbohydrates (lipids), 030104 developmental biology, Cell signalling, rôle protecteur
Abstract

Cardioprotective effects of dietary anthocyanins are partly attributed to their ability to maintain endothelial function. However, the underlying cellular and molecular mechanisms of action are not fully understood. This study aimed to evaluate the effect of anthocyanins and their gut metabolites, at physiologically-relevant conditions, on endothelial cell (EC) function and decipher the underlying molecular mechanisms of action using integrated omics approaches. Primary EC were treated with a mixture of 0.1 mu M cyanidin-3-arabinoside, 0.1 mu M cyanidin-3-galactoside, 0.1 mu M cyanidin-3-glucoside, 0.1 mu M delphinidin-3-glucoside, 0.1 mu M peonidin-3-glucoside and 0.5 mu M 4-hydroxybenzaldehyde for 3 h or a mixture of gut metabolites: 0.2 mu M protocatechuic, 2 mu M vanillic, 1 mu M ferulic and 2 mu M hippuric acids for 18 h. Also, successive exposure of EC to both mixtures was performed to mimic anthocyanin pharmacokinetics following their intake. Inflammatory stress was induced using TNF alpha and monocytes added to assess adhesion and transmigration. Effects of these mixtures on gene, miRNA expression and their potential interaction with cell signalling were investigated. Anthocyanins and their gut metabolites significantly reduced monocyte adhesion and transendothelial migration. Gene expression analysis, using macroarrays, showed that tested compounds modulated the expression of genes involved in cell-cell adhesion, cytoskeleton organisation or focal adhesion. Bioinformatics analyses of gene expression data identified potential transcription factors involved in the observed nutrigenomic effects and signalling proteins regulating their activity. Molecular docking revealed cell signalling proteins to which these bioactives may bind to and potentially affect their activity and the activation of downstream signalling, effects that were in agreement with the results of Western blot analyses. Microarray analysis showed that anthocyanins and their gut metabolites affected miRNA expression in EC, especially those involved in regulation of EC permeability, contributing to the observed changes in EC function. Integration of these results revealed endothelial-protective properties of anthocyanins and their gut metabolites and deciphered new underlying multi-target and multi-layered mode of action.

Published
2018
Full Text
View/download PDF

16. Substantial variability across individuals in the vascular and nutrigenomic response to an acute intake of curcumin: A randomizsed controlled trial

Author

Jean René Lusson, Bruno Pereira, Damien Richard, Andrzej Mazur, Claude Dubray, Céline Lambert, Nicolas Barber-Chamoux, Christine Morand, Véronique Habauzit, Marie-Anne Verny, Dragan Milenkovic, Céline Boby, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Délégation à la Recherche Clinique et à l'Innovation (DRCI), CHU Clermont-Ferrand, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre d’Investigation Clinique (CIC), Institut National de la Santé et de la Recherche Médicale (INSERM), Université d'Auvergne - Clermont-Ferrand I (UdA), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), service de Biostatistiques, DRCI, Centre Hospitalier Universitaire de Clermont-Ferrand, Unité Biostatistique, Institut de Recherche en Gestion et en Economie (IREGE), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Institut Pascal - Clermont Auvergne (IP), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Université d'Auvergne (Clermont Ferrand 1) (UdA), CIC 501, Université Clermont Auvergne (UCA), Biostatistics Unit (DRCI), Clinical pharmacology, Cardiologie, Hôpital Privé Jacques Cartier [Massy], UMR 766, Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité de Biostatistiques [CHU Clermont-Ferrand], Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), CHU Clermont-Ferrand-CHU Clermont-Ferrand, Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Délégation à la Recherche Clinique et à l'Innovation ( DRCI ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Centre d’Investigation Clinique ( CIC ), Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université d'Auvergne (Clermont Ferrand 1) ( UdA ), Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), ARCA (Association de Recherche en Cardiologie d'Auvergne), Unité de Nutrition Humaine ( UNH ), Institut National de la Recherche Agronomique ( INRA ) -Université d'Auvergne - Clermont-Ferrand I ( UdA ) -Clermont Université, Institut de Recherche en Gestion et en Economie ( IREGE ), Université Savoie Mont Blanc ( USMB [Université de Savoie] [Université de Chambéry] ), Institut Pascal - Clermont Auvergne ( IP ), Sigma CLERMONT ( Sigma CLERMONT ) -Université Clermont Auvergne ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Santé et de la Recherche Médicale, Centre de Recherche en Nutrition Humaine (CRNH). FRA., ProdInra, Archive Ouverte, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, 0301 basic medicine, Pathology, Physiology, fonction endothéliale, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, 030232 urology & nephrology, Individuality, vascular system, 030204 cardiovascular system & hematology, nutrigenomic, law.invention, essai clinique, chemistry.chemical_compound, Nutrigenomics, 0302 clinical medicine, Randomized controlled trial, endothelial function, law, curcumin, nutrigénomique, ComputingMilieux_MISCELLANEOUS, Cross-Over Studies, [ INFO.INFO-IM ] Computer Science [cs]/Medical Imaging, blood pressure, food and beverages, clinical trial, Middle Aged, 3. Good health, Vasodilation, Population study, Female, Cardiology and Cardiovascular Medicine, mode d'apport, Biotechnology, medicine.medical_specialty, Subgroup analysis, Placebo, nutragenomics, random selection, inter-individual variability, 03 medical and health sciences, Double-Blind Method, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Internal medicine, Internal Medicine, medicine, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Humans, randomisation, 030109 nutrition & dietetics, business.industry, système vasculaire, Crossover study, Clinical trial, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, curcumine, Blood pressure, chemistry, Curcumin, Endothelium, Vascular, Transcriptome, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract

cope Curcumin exerts biological activities of interest in cardiovascular prevention. However, its vascular protective effect is still poorly investigated in humans. The present study aims to assess vasculat effect of an acute intake of curcumin and its nutrigenomic impact in circulating immune cells. Methods and results In a randomised, double-blind, crossover design, eighteen healthy smokers consumed a placebo or a 5-gram of curcumin. Before and two hours after the intake, vascular function measurements were performed by using flow-mediated dilation (FMD). In addition, endothelial function in the microcirculation and blood pressure were evaluated. Plasma curcumin concentrations and changes in gene expression in peripheral blood mononuclear cells (PBMC) were analysed. No significant effect of curcumin on FMD was observed when considering the entire study population, mainly due to a high inter-individual variability. A subgroup analysis according to the gender or the cardiovascular-risk score revealed a significant effect of curcumin on FMD in women and in subjects presenting lower cardiovascular risk. No change in gene expression was observed when data were analysed for all volunteers but changes in expression were observed when analyzed according to gender. Conclusions This clinical trial highlights that a substantial variability in efficacy of curcumin exists across individuals. This article is protected by copyright. All rights reserved

Published
2017
Full Text
View/download PDF

17. Molecular regulation of high muscle mass in developing Blonde d'Aquitaine cattle fetuses

Author

Antonio Reverter, Brigitte Picard, Isabelle Cassar-Malek, Nicholas J. Hudson, Céline Boby, Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
0301 basic medicine, Gene isoform, medicine.medical_specialty, QH301-705.5, Science, [SDV]Life Sciences [q-bio], Myostatin, Biology, General Biochemistry, Genetics and Molecular Biology, genomic, race bovine blonde d'aquitaine, 03 medical and health sciences, Internal medicine, myostatine, Myosin, Gene expression, medicine, Biology (General), skeletal muscle, CSRP3, [ SDV ] Life Sciences [q-bio], Myogenesis, 0402 animal and dairy science, Skeletal muscle, muscle squelettique, 04 agricultural and veterinary sciences, Genomics, myogénèse, 040201 dairy & animal science, Molecular biology, mitochondria, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, voluntary muscle, mitochondrie, myostatin, fœtus amnion fetus yolk sac, biology.protein, MYH7, myogenesis, fœtus, General Agricultural and Biological Sciences, Research Article
Abstract

The Blonde d'Aquitaine (BA) is a French cattle breed with enhanced muscularity, partly attributable to a MSTN mutation. The BA m. Semitendinosus has a faster muscle fibre isoform phenotype comprising a higher proportion of fast type IIX fibres compared to age-matched Charolais (CH). To better understand the molecular network of modifications in BA compared to CH muscle, we assayed the transcriptomes of the m. Semitendinosus at 110, 180, 210 and 260 days postconception (dpc). We used a combination of differential expression (DE) and regulatory impact factors (RIF) to compare and contrast muscle gene expression between the breeds. Prominently developmentally regulated genes in both breeds reflected the replacement of embryonic myosin isoforms (MYL4, MYH3) with adult isoforms (MYH1) and the upregulation of mitochondrial metabolism (CKMT2, AGXT2L1) in preparation for birth. However, the transition to a fast, glycolytic muscle phenotype in the MSTN mutant BA is detectable through downregulation of various slow twitch subunits (TNNC1, MYH7, TPM3, CSRP3) beyond 210 dpc, and a small but consistent genome-wide reduction in mRNA encoding the mitoproteome. Across the breeds, NRIP2 is the regulatory gene possessing a network change most similar to that of MSTN., Summary: We explored the molecular basis of high muscle mass in Blonde d’Aquitaine cattle, and found that mRNA encoding slow fibres and mitochondrial proteins were downregulated in this breed.

Published
2017
Full Text
View/download PDF

18. Mammary gland transcriptome and proteome modifications by nutrient restriction in early lactation Holstein cows challenged with intramammary lipopolysaccharide

Author

Karol Pawłowski, José A.A. Pires, Yannick Faulconnier, Christophe Chambon, Pierre Germon, Céline Boby, Christine Leroux, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), American Society of Animal Science (ASAS). USA., American Dairy Science Association (ADSA). USA., Canadian Society of Animal Science (CSAS). CAN., Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), GISA meta-program of INRA (Ruminflame project), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
Lipopolysaccharides, Proteomics, mammary gland, [SDV]Life Sciences [q-bio], approche protéomique, plateforme exploration du métabolisme Clermont-Ferrand, Article, [SHS]Humanities and Social Sciences, lcsh:Chemistry, cows, Mammary Glands, Animal, Nutrigenomics, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Animals, Lactation, [INFO]Computer Science [cs], [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, analyse du transcriptome, lcsh:QH301-705.5, transcriptomic analysis, ComputingMilieux_MISCELLANEOUS, Caloric Restriction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Nutritional Requirements, race holstein, lipopolisaccharide, proteomic analysis, lcsh:Biology (General), lcsh:QD1-999, Gene Expression Regulation, inflammation, mammary omics, undernutrition, inflammation, glande mammaire, restriction alimentaire, Cattle, Female
Abstract

The objective is to study the effects of nutrient restrictions, which induce a metabolic imbalance on the inflammatory response of the mammary gland in early lactation cows. The aim is to decipher the molecular mechanisms involved, by comparing a control, with a restriction group, a transcriptome and proteome, after an intra-mammary lipopolysaccharide challenge. Multi-parous cows were either allowed ad libitum intake of a lactation diet (n = 8), or a ration containing low nutrient density (n = 8; 48% barley straw and dry matter basis) for four days starting at 24 ± 3 days in milk. Three days after the initiation of their treatments, one healthy rear mammary quarter of 12 lactating cows was challenged with 50 µg of lipopolysaccharide (LPS). Transcriptomic and proteomic analyses were performed on mammary biopsies obtained 24 h after the LPS challenge, using bovine 44K microarrays, and nano-LC-MS/MS, respectively. Restriction-induced deficits in energy, led to a marked negative energy balance (41 versus 97 ± 15% of Net Energy for Lactation (NEL) requirements) and metabolic imbalance. A microarray analyses identified 25 differentially expressed genes in response to restriction, suggesting that restriction had modified mammary metabolism, specifically β-oxidation process. Proteomic analyses identified 53 differentially expressed proteins, which suggests that the modification of protein synthesis from mRNA splicing to folding. Under-nutrition influenced mammary gland expression of the genes involved in metabolism, thereby increasing β-oxidation and altering protein synthesis, which may affect the response to inflammation.

Published
2016
Full Text
View/download PDF

19. TRF2/RAP1 and DNA–PK mediate a double protection against joining at telomeric ends

Author

Eric Gilson, Philippe Frit, Marie-Josèphe Giraud-Panis, Oriane Bombarde, Céline Boby, Bernard Salles, Dennis Gomez, and Patrick Calsou

Subjects
Genome instability, DNA Ligases, DNA Repair, DNA repair, Telomere-Binding Proteins, DNA-Activated Protein Kinase, LIG4, Biology, Article, Genomic Instability, Shelterin Complex, General Biochemistry, Genetics and Molecular Biology, DNA Ligase ATP, Humans, Telomeric Repeat Binding Protein 2, Molecular Biology, chemistry.chemical_classification, Telomere-binding protein, DNA ligase, General Immunology and Microbiology, General Neuroscience, fungi, DNA, Telomere, Molecular biology, Non-homologous end joining, enzymes and coenzymes (carbohydrates), chemistry, embryonic structures, Rap1, HeLa Cells
Abstract

DNA-dependent protein kinase (DNA-PK) is a double-strand breaks repair complex, the subunits of which (KU and DNA-PKcs) are paradoxically present at mammalian telomeres. Telomere fusion has been reported in cells lacking these proteins, raising two questions: how is DNA-PK prevented from initiating classical ligase IV (LIG4)-dependent non-homologous end-joining (C-NHEJ) at telomeres and how is the backup end-joining (EJ) activity (B-NHEJ) that operates at telomeres under conditions of C-NHEJ deficiency controlled? To address these questions, we have investigated EJ using plasmid substrates bearing double-stranded telomeric tracks and human cell extracts with variable C-NHEJ or B-NHEJ activity. We found that (1) TRF2/RAP1 prevents C-NHEJ-mediated end fusion at the initial DNA-PK end binding and activation step and (2) DNA-PK counteracts a potent LIG4-independent EJ mechanism. Thus, telomeres are protected against EJ by a lock with two bolts. These results account for observations with mammalian models and underline the importance of alternative non-classical EJ pathways for telomere fusions in cells.

Published
2010
Full Text
View/download PDF

20. 0129 Liver transcriptome modifications by nutrient restriction in early lactation Holstein cows challenged with intramammary lipopolysaccharide

Author

K. Pawlowski, Yannick Faulconnier, Denys Durand, J.A.A. Pires, A. de la Foye, Céline Boby, and Christine Leroux

Subjects
medicine.medical_specialty, Lipopolysaccharide, General Medicine, Biology, Andrology, Transcriptome, chemistry.chemical_compound, Nutrient, medicine.anatomical_structure, Endocrinology, chemistry, Internal medicine, Lactation, Genetics, medicine, Animal Science and Zoology, Food Science
Published
2016
Full Text
View/download PDF

21. 0131 Mammary gland transcriptome and proteome modifications by nutrient restriction in early lactation Holstein cows challenged with intramammary lipopolysaccharide

Author

Christine Leroux, K. Pawlowski, Yannick Faulconnier, A. de la Foye, J.A.A. Pires, Christophe Chambon, and Céline Boby

Subjects
medicine.medical_specialty, Lipopolysaccharide, Mammary gland, General Medicine, Biology, Transcriptome, Andrology, chemistry.chemical_compound, medicine.anatomical_structure, Endocrinology, Nutrient, chemistry, Internal medicine, Lactation, Proteome, Genetics, medicine, Animal Science and Zoology, Food Science
Published
2016
Full Text
View/download PDF

22. Molecular regulation of muscle mass in developing Blond Aquitaine foetuses compared to Charolais

Author

Isabelle Cassar-Malek, Anne de la Foye, Céline BOBY, Brigitte Picard, Antonio Reverter, Nick Hudson, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), ICMS Australasia. Sydney, AUS., and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
transcriptomics, cattle, myostatin, [SDV.BA]Life Sciences [q-bio]/Animal biology, development, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

23. Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways

Author

Christine Morand, Sylvain Claude, Jeremy P. E. Spencer, Dragan Milenkovic, Céline Boby, Nicolas Gérard, Ana Rodriguez-Mateos, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, University of Reading (UOR), European Project: 226588,EC:FP7:KBBE,FP7-KBBE-2008-2B,FLAVIOLA(2009), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
Flavonols, métabolite de flavanol, Sulfuric Acid Esters, Biology, p38 Mitogen-Activated Protein Kinases, Catechin, Monocytes, Focal adhesion, Transcriptome, Nutrigenomics, adhésion de monocyte, Cell Adhesion, Human Umbilical Vein Endothelial Cells, medicine, Humans, Phosphorylation, Cell adhesion, Tumor Necrosis Factor-alpha, Monocyte, Transcription Factor RelA, Endothelial Cells, Adhesion, Endothelial stem cell, medicine.anatomical_structure, Biochemistry, Tumor necrosis factor alpha, cellule endothéliale, Endothelium, Vascular, Signal transduction, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Signal Transduction, Transcription Factors, Food Science, Biotechnology, expression des gènes
Abstract

Scope Consumption of flavanol-rich foods is associated with an improvement in endothelial function. However, the specific biologically active flavanol metabolites involved in this benefit, as well as their molecular mechanisms of action have not been identified. The aim of this work was to examine the effect of plasma flavanol metabolites on adhesion of monocytes to TNF-α-activated endothelial cells and identify potential underlying mechanisms. Methods and results 4′-O-methyl(−)-epicatechin, 4′-O-methyl(−)-epicatechin-7-β-d-glucuronide, and (−)­epicatechin-4′-sulfate decreased the adhesion of monocytes to endothelial cells at physiologically relevant concentrations, from 0.2 to 1 μM. Transcriptomic studies showed that each of the flavanol metabolites affected the expression of different genes in endothelial cells. However, these genes are involved in the cellular processes that control adhesion and migration of monocytes to vascular endothelium, most notably those regulating cell adhesion, cell–cell junctions, focal adhesion, and cytoskeleton remodeling. Gene expression profiles obtained suggest lower monocyte recruitment, in agreement with results from cell adhesion assays. The nutrigenomic effect of metabolites seems to be mediated through their capacity to modulate phosphorylation of p65 and p38 cell-signaling proteins. Conclusion Our study provides findings into the molecular mechanisms by which plasma flavanol metabolites could be efficient to preserve vascular endothelium integrity in nutritionally relevant conditions.

Published
2014
Full Text
View/download PDF

24. Dietary flavanols modulate the transcription of genes associated with cardiovascular pathology without changes in their DNA methylation state

Author

François Fuks, Ken Declerck, Guido R.M.M. Haenen, Katarzyna Szarc vel Szic, Martin Bizet, Guy Haegeman, Matthieu Defrance, Antje R. Weseler, Aalt Bast, Christine Leroux, Dragan Milenkovic, Céline Boby, Emilie Calonne, Wim Vanden Berghe, Kris Laukens, Karen Heyninck, Sarah Dedeurwaerder, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Universiteit Gent = Ghent University (UGENT), PPES, Department of Biomedical Sciences, University of Antwerp (UA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Department of Mathematics and Computer Science, Laboratory of Cancer Epigenetics, ULB-Cancer Research Centre, Maastricht University [Maastricht], International Nutrition Company (INC) BV, Loosdrecht, The Netherlands, European Union [226588], University of Antwerp, Belgium, Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Universiteit Gent = Ghent University [Belgium] (UGENT), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Farmacologie en Toxicologie, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, and RS: CARIM - R3 - Vascular biology

Subjects
Male, Transcription, Genetic, Microarrays, BLOOD MONONUCLEAR-CELLS, DISEASE, 0302 clinical medicine, Animal Cells, Transcription (biology), Gene expression, mécanisme, lcsh:Science, Immune Response, Cells, Cultured, Oligonucleotide Array Sequence Analysis, 0303 health sciences, NF-kappa B, food and beverages, Genomics, Sciences bio-médicales et agricoles, 3. Good health, FACTOR-KAPPA-B, Bioassays and Physiological Analysis, EPIGENETIC MECHANISMS, Cardiovascular Diseases, 030220 oncology & carcinogenesis, Epigenetics, Flavonoids -- administration & dosage, Cellular Types, Grape Seed Extract -- administration & dosage, SMOKING, Immune Cells, Immunology, Cardiovascular Diseases -- genetics -- metabolism -- prevention & control, Médecine humaine et pathologie, maladie, 03 medical and health sciences, POLYPHENOLS, épigénétique, adhésion cellulaire, Human Umbilical Vein Endothelial Cells, Genetics, Humans, Biology, Reporter gene, lcsh:R, fungi, Computational Biology, Biology and Life Sciences, Human Umbilical Vein Endothelial Cells -- drug effects -- physiology, DNA Methylation, lcsh:Q, CpG Islands, Human health and pathology, Human medicine, lcsh:Medicine, Gene Expression, Leukocytes -- metabolism, EXPRESSION PROFILES, Molecular Cell Biology, Leukocytes, Medicine and Health Sciences, NF-kappa B -- metabolism, profil d'expression, cellule endotheliale vasculaire, Multidisciplinary, Middle Aged, Clinical Laboratory Sciences, DNA methylation, DNA microarray, Transcriptome Analysis, Engineering sciences. Technology, nf kappa b, Research Article, Adult, Transcription, Genetic -- drug effects, Cardiology, LEUKOCYTE ADHESION, Research and Analysis Methods, polyphénol, Diagnostic Medicine, échantillon sanguin, Cell Adhesion, leucocyte, Transcription factor, Gene, Nutrition, 030304 developmental biology, Flavonoids, Grape Seed Extract, Cell Biology, Sequence Analysis, DNA, Genome Analysis, Molecular biology, ENDOTHELIAL-CELLS, Coculture Techniques, Gene Expression Regulation, PATTERNS, Clinical Immunology, Genome Expression Analysis, Transcriptome, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, cellule mononuclee
Abstract

In a recent intervention study, the daily supplementation with 200 mg monomeric and oligomeric flavanols (MOF) from grape seeds for 8 weeks revealed a vascular health benefit in male smokers. The objective of the present study was to determine the impact of MOF consumption on the gene expression profile of leukocytes and to assess changes in DNA methylation., info:eu-repo/semantics/published

Published
2014
Full Text
View/download PDF

25. Cocoa flavanol metabolites modulate signaling pathways and expression of genes involved in monocyte adhesion and transendothelial migration

Author

Dragan Milenkovic, Céline Boby, Dominique Bayle, Christine Morand, Sylvain Claude, Nicolas Gérard, Unité de Nutrition Humaine (UNH), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)

Subjects
Monocyte adhesion, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Transendothelial migration, Chemistry, Physiology (medical), Immunology, Signal transduction, Biochemistry, Gene, [ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences, Cell biology
Abstract

Suppl. 2Suppl. 2; absent

Published
2012
Full Text
View/download PDF

26. TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends

Author

Oriane Bombarde, Céline BOBY, Dennis Gomez, Philippe Frit, Marie-Josèphe Giraud-Panis, Eric Gilson, Bernard Salles, Patrick Calsou, Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire Joliot Curie, École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Moléculaire de la Cellule (LBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Laboratory of Biology and Pathology of Genomes, Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biologie et pathologie des génomes, and Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
enzymes and coenzymes (carbohydrates), embryonic structures, fungi, DNA repair, telomeres Introduction, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, genome instability, non-homologous end-joining
Abstract

DNA-dependent protein kinase (DNA-PK) is a double-strand breaks repair complex, the subunits of which (KU and DNA-PKcs) are paradoxically present at mammalian telomeres. Telomere fusion has been reported in cells lacking these proteins, raising two questions: how is DNA-PK prevented from initiating classical ligase IV (LIG4)-dependent non-homologous end-joining (C-NHEJ) at telomeres and how is the backup end-joining (EJ) activity (B-NHEJ) that operates at telomeres under conditions of C-NHEJ deficiency controlled? To address these questions, we have investigated EJ using plasmid substrates bearing double-stranded telomeric tracks and human cell extracts with variable C-NHEJ or B-NHEJ activity. We found that (1) TRF2/RAP1 prevents C-NHEJ-mediated end fusion at the initial DNA-PK end binding and activation step and (2) DNA-PK counteracts a potent LIG4-independent EJ mechanism. Thus, telomeres are protected against EJ by a lock with two bolts. These results account for observations with mammalian models and underline the importance of alternative non-classical EJ pathways for telomere fusions in cells.

Published
2010

27. RhoB protects human keratinocytes from UVB-induced apoptosis through epidermal growth factor receptor signaling

Author

Anne Pradines, Marie Charveron, Isabelle Lajoie-Mazenc, Gilles Favre, Bruno Canguilhem, Céline Boby, and Caroline Baudouin

Subjects
Keratinocytes, DNA, Complementary, Time Factors, Transcription, Genetic, Cell Survival, Ultraviolet Rays, RHOB, Blotting, Western, Immunoblotting, Apoptosis, Enzyme-Linked Immunosorbent Assay, Biology, Biochemistry, Glycogen Synthase Kinase 3, Epidermal growth factor, RhoB GTP-Binding Protein, medicine, Phosphorylation, RNA Processing, Post-Transcriptional, RNA, Small Interfering, skin and connective tissue diseases, Promoter Regions, Genetic, rhoB GTP-Binding Protein, Molecular Biology, Protein kinase B, Cytoskeleton, Glycogen Synthase Kinase 3 beta, integumentary system, Cell Death, Reverse Transcriptase Polymerase Chain Reaction, Cell Biology, Cell biology, ErbB Receptors, medicine.anatomical_structure, UVB-induced apoptosis, Cancer research, RNA, Tumor promotion, Electrophoresis, Polyacrylamide Gel, Guanosine Triphosphate, Signal transduction, Keratinocyte, DNA Damage, Signal Transduction
Abstract

Next Section Abstract Exposure of the skin to UVB light results in the formation of DNA photolesions that can give rise to cell death, mutations, and the onset of carcinogenic events. Specific proteins are activated by UVB and then trigger signal transduction pathways that lead to cellular responses. An alteration of these signaling molecules is thought to be a fundamental event in tumor promotion by UVB irradiation. RhoB, encoding a small GTPase has been identified as a DNA damage-inducible gene. RhoB is involved in epidermal growth factor (EGF) receptor trafficking, cytoskeletal organization, cell transformation, and survival. We have analyzed the regulation of RhoB and elucidated its role in the cellular response of HaCaT keratinocytes to relevant environmental UVB irradiation. We report here that the activated GTP-bound form of RhoB is increased rapidly within 5 min of exposure to UVB, and then RhoB protein levels increased concomitantly with EGF receptor (EGFR) activation. Inhibition of UVB-induced EGFR activation prevents RhoB protein expression and AKT phosphorylation but not the early activation of RhoB. Blocking UVB-induced RhoB expression with specific small interfering RNAs inhibits AKT and glycogen synthase kinase-3β phosphorylation through inhibition of EGFR expression. Moreover, down-regulation of RhoB potentiates UVB-induced cell apoptosis. In contrast, RhoB overexpression protects keratinocytes against UVB-induced apoptosis. These results indicated that RhoB is regulated upon UVB exposure by a two-step process consisting of an early EGFR-independent RhoB activation followed by an EGFR-dependent induction of RhoB expression. Moreover, we have demonstrated that RhoB is essential in regulating keratinocyte cell survival after UVB exposure, suggesting its potential role in photocarcinogenesis.

Published
2005

28. Update of ProteINSIDE: an online tool for proteome data mining

Author

Jérémy Tournayre, Céline BOBY, Matthieu Matthieu.Reichstadt@inrae.Fr Reichstadt, Muriel Bonnet, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

29. Une approche intégrée pour comprendre le dialogue entre tissus/organes chez les ruminants producteurs

Author

Isabelle Cassar-Malek, Mylène Delosière, Laurence Bernard, Céline BOBY, Carole Delavaud, Denis Durand, Yannick Faulconnier, Christine Leroux, Anne Listrat, Brigitte Picard, José Pires, Muriel Bonnet, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

30. Use of nutrigenomics to provide new insights in the molecular mechanisms involved in the effects of grapefruit polyphenols on vascular health in humans

Author

Dragan Milenkovic, Veronique Habauzit, Laurent-Emmanuel Monfoulet, Marie-Anne Verny, Dominique Bayle, Séverine Valero, Céline BOBY, Nicolas Barber Chamoux, André Mazur, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
polyphénol, protection vasculaire, [SDV]Life Sciences [q-bio], food and beverages, jus de pamplemousse, santé humaine, nutragenomics, human health, nutrigénomique, mécanisme moléculaire
Abstract

Use of nutrigenomics to provide new insights in the molecular mechanisms involved in the effects of grapefruit polyphenols on vascular health in humans. 3. Scientific Workshop

31. Exploitation bioinformatique de la comparaison des transcriptomes de tissus adipeux de chèvres lactantes et non lactantes: Rapport de stage DUT Génie Biologique -BioInformatique 2ème Année 2015/2016

Author

Gestin, Yannick, Faulconnier, Yannick, Boby, Céline, Leroux, Christine, Institut Universitaire de Technologie - Clermont-Ferrand (IUT Clermont-Ferrand), Université Clermont Auvergne (UCA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université d'Auvergne - Clermont-Ferrand I (UdA), Clermont-Ferrand, FRA., Yannick Faulconnier, Céline Boby, and ANR-12-BSV6-0013,MilkChEST,Approche intégrée des voies de sécrétion de la cellule épithéliale mammaire: adaptabilité de la matière grasse laitière(2012)

Subjects
[SDV]Life Sciences [q-bio], Tissu adipeux, Lactaction, RNA-SEQ, Bioanalyse, Chèvre
Abstract

Licence; The objective of this study was to compare the lipid storage mechanisms in adipose tissue of lactating goats (in phase of mobilization of fat reserves) and non-lactating (lipid storage phase). A high-throughput RNA sequencing (RNA-seq) extracted from adipose tissue showed that 252 genes were differentially expressed. We have exploited these results using several bioinformatics tools. A Gene Ontological (GO) analyzes allowed classifying these Differentially Expressed Genes (DEG) in 14 biological processes. Enrichment tests were then performed and revealed an enrichment of GO associated with lipid metabolism. Metabolic pathways and network construction enrichment tools have enabled us to identify the pathways of fatty acid and their degradation, specifically targeting two key regulators, leptin and HNF4A transcription factor. Thus, we were able to show that these DEG in the adipose tissue of dairy goats are mostly involved in oxidation and lipid synthesis. These results are just the first hypotheses and these analyses must be performed by confronting with zootechnic data and caprine annotations. The operating strategy of the data could be used for the analysis of new RNA-seqdata.; Exploitation bioinformatique de la comparaison des transcriptomes de tissus adipeux de chèvres lactantes et non lactantesL’objectif de cette étude a été de comparer les mécanismes de stockage lipidique dans les tissus adipeux de chèvres lactantes (en phase de mobilisation des réserves lipidiques) et non lactantes (en phase de stockage lipidique). Un séquençage haut débit des ARN (RNA-seq) extraits du tissu adipeux a montré que 252 gènes étaient différentiellement exprimés (GDE). Nous avons exploité ces résultats à l’aide de plusieurs outils bio-informatiques. Une analyse Gene Ontologique (GO) a permis de classer ces GDE dans 14 processus biologiques. Des tests d’enrichissement ont mis en évidence un enrichissement des GO associés au métabolisme lipidique. Des outils d’enrichissement de voies métaboliques et de construction de réseaux nous ont ensuite permis d’identifier les pathways des acides gras et de leur dégradation en ciblant notamment deux régulateurs clés, la leptine et le facteur de transcription HNF4A. Nous avons ainsi pu mettre en évidence que ces GDE dans les tissus adipeux de chèvres laitières sont surtout impliqués dans l’oxydation et la synthèse des lipides. Ces résultats ne sont que des premières hypothèses et ces analyses devront être poursuivies en les confrontant aux données zootechniques et aux annotations caprines. Cette stratégie d’exploitation des données pourra servir à l’analyse de nouvelles données RNA-seq

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results