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1. Contributors

Author

Amy P. Abernethy, Douglas G. Adler, Yesne Alici, Eugene Balagula, Ani Balmanoukian, Nikhil Banerjee, Gerhild Becker, Virginia Boquiren, Julie R. Brahmer, William Breitbart, Michael T. Brennan, Eduardo Bruera, Marianne Brydøy, Robert Buckman, Amanda Caissie, Joseph R. Carver, Harvey M. Chochinov, Edward Chow, Ai-Ping Chua, Maureen E. Clark, Raimundo Correa, Kerry S. Courneya, David C. Currow, Shalini Dalal, Mellar P. Davis, Maike de Wit, Haryana Dhillon, Mario Dicato, Ingo J. Diel, Jason E. Dodge, Matthew Doolittle, Wolfgang Dörr, Geoffrey P. Dunn, Alexandra M. Easson, Edzard K. Ernst, Petra Ch. Feyer, David R. Fogelman, Sophie D. Fosså, Orit Freedman, Debra L. Friedman, Surafel Gebreselassie, Thomas R. Gildea, Marc Giovannini, Paul A. Glare, Arin K. Greene, Janet R. Hardy, Daniel B. Hinshaw, Ulrike Hoeller, Juliet Hou, Lynn Jedlicka, Siri Beier Jensen, Katherine T. Johnston, Jason M. Jones, Karin Jordan, Karunakaravel Karuppasamy, Raghid Kikano, Kenneth L. Kirsh, Cecilie Kiserud, David W. Kissane, Małgorzata Krajnik, Christof Kramm, Sheldon Kwok, Mario E. Lacouture, Abraham Levitin, Madeline Li, S. Lawrence Librach, Wendy G. Lichtenthal, Isador Lieberman, Vernon W.H. Lin, Hartmut Link, Christopher Lo, César V. Lopes, Charles L. Loprinzi, Amy E. Lowery, Robert Mader, Henriette Magelssen, Vincent Maida, H.A. Marsman, Susan E. McClement, Erin L. McGowan, Daniel J. Moskovic, Marissa Newman, Tanya Nikolova, Jan Oldenburg, Petra Ortner, Dierdre R. Pachman, Jocelyn Pang, Steven D. Passik, Timothy M. Pawlik, Júlio C. Pereira-Lima, Douglas E. Peterson, Barbara F. Piper, Laurent Plawny, Kathy Pope, Jennifer Potter, Holly G. Prigerson, Carla I. Ripamonti, Lizbeth Robles, Gary Rodin, Lisa Ruppert, Brenda M. Sabo, Nadia Salvo, Jose Fernando Santacruz, Josée Savard, Carolyn C. Schook, Dale R. Shepard, Heather L. Shepherd, Sumner A. Slavin, Martin L. Smith, Fred K.L. Spijkervet, Glen H.J. Stevens, Michael D. Stubblefield, Nigel P. Sykes, Matthew Tam, Martin H.N. Tattersall, Mary L.S. Vachon, A.E. van der Pool, T.M. van Gulik, Janette Vardy, Cornelis Verhoef, Arjan Vissink, Hans-Heinrich Wolf, Rebecca K.S. Wong, Camilla Zimmermann, and Zbigniew Zylicz

Published
2011
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2. Expression of mucin-2 and correlation with PAS-alcian blue stain in Barrett's esophagus

Author

César V, Lopes, Júlio, Pereira-Lima, and Antônio, Hartmann

Subjects
Adult, Aged, 80 and over, Male, Mucin-2, Staining and Labeling, Mucins, Middle Aged, Periodic Acid-Schiff Reaction, Immunohistochemistry, Barrett Esophagus, Humans, Female, Alcian Blue, Coloring Agents, Aged
Abstract

Hematoxylin-eosin stain may fail to show intestinal metaplasia in Barrett's esophagus, and PAS-alcian blue may present difficulties of interpretation due to its more heterogeneous staining. We investigated whether Mucin-2 is a good substitute for PAS-alcian blue in the detection of goblet cells.Biopsy specimens from 47 Barrett's esophagus patients were stained with hematoxylineosin and PAS-alcian blue. Mucin-2 expression was evaluated with monoclonal antibody. The intra- and interobserver agreements about the expression of Mucin-2 and PAS-alcian blue stain were determined by kappa statistics.PAS-alcian blue and Mucin-2 were positive in all cases. Mucin expression was positive in goblet cells and few columnar cells. PAS-alcian blue showed heterogeneous staining. The columnar epithelium and the submucosal glands were also stained. Intra- and interobserver agreement in the identification of intestinal metaplasia was 100%.Mucin-2 agreed fully with PAS-alcian blue. The intra- and interobserver agreement was perfect, justifying its use in the diagnosis of Barrett's esophagus.

Published
2006

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