1. In vitro phannacodynaniics of enrofloxacin against an Escherichia coli gyrA mutant
- Author
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Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı., Cengiz, Murat, Sorucu, Ali, Arslan, Erdem, and ABE-5935-2020
- Subjects
Veterinary sciences ,Turkey ,Streptococcus-pneumoniae ,Escherichia Coli ,Plasmids ,Enterobacteriaceae ,C-T mutation ,Fluoroquinolone resistance ,Pharmacodynamic of enrofloxacin ,Quinolone resistance ,Microdilution ,Colony-variant phenotype ,Significant ,Escherichia coli ,Mechanisms ,Animals ,Staphylococcus-aureus ,Selection ,Mutations ,Model - Abstract
The aim of this study was to investigate in vitro the pharmacodynamics of Enrofloxacin (ENR) against an Escherichia coli gyrA mutant (E. coli MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) and multi-step resistance selection was performed until reaching 1 mu g ML(-1) MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for E. coli MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations >= 8 times the MIC with no significant regrowth by 24 h. European Cooperation in Science and Technology (COST) BM0701
- Published
- 2011