Search

Macrophage infectivity potentiator (MIP) proteins are widespread in human pathogens includingLegionella pneumophila, the causative agent of Legionnaires’ disease and protozoans such asTrypanosoma cruzi. All MIP proteins contain a FKBP (FK506 binding protein)-like prolyl-cis/trans-isomerase domain that hence presents an attractive drug target. Some MIPs such as theLegionellaprotein (LpMIP) have additional appendage domains of mostly unknown function. In full-length, homodimericLpMIP, the N-terminal dimerization domain is linked to the FKBP-like domain via a long, free-standing stalk helix. Combining X-ray crystallography, NMR and EPR spectroscopy and SAXS, we elucidated the importance of the stalk helix for protein dynamics and inhibitor binding to the FKBP-like domain and bidirectional crosstalk between the different protein regions. The first comparison of a microbial MIP and a human FKBP in complex with the same synthetic inhibitor was made possible by high-resolution structures ofLpMIP with a [4.3.1]-aza-bicyclic sulfonamide and provides a basis for designing pathogen-selective inhibitors. Through stereospecific methylation, the affinity of inhibitors to toL. pneumophilaandT. cruziMIP was greatly improved. The resulting X-ray inhibitor-complex structures ofLpMIP and TcMIP at 1.49 and 1.34 Å, respectively, provide a starting point for developing potent inhibitors against MIPs from multiple pathogenic microorganisms.

The aims of this study were to 1) determine if continuous eruption occurs in the maxillary teeth, 2) assess the magnitude of the continuous eruption, and 3) evaluate the effects of continuous eruption on the different periodontal parameters by using data from the population-based cohort of the Study of Health in Pomerania (SHIP). The jaw casts of 140 participants from the baseline (SHIP-0) and 16-y follow-up (SHIP-3) were digitized as 3-dimensional models. Robust reference points were set to match the tooth eruption stage at SHIP-0 and SHIP-3. Reference points were set on the occlusal surface of the contralateral premolar and molar teeth, the palatal fossa of an incisor, and the rugae of the hard palate. Reference points were combined to represent 3 virtual occlusal planes. Continuous eruption was measured as the mean height difference between the 3 planes and rugae fix points at SHIP-0 and SHIP-3. Probing depth, clinical attachment levels, gingiva above the cementoenamel junction (gingival height), and number of missing teeth were clinically assessed in the maxilla. Changes in periodontal variables were regressed onto changes in continuous eruption after adjustment for age, sex, number of filled teeth, and education or tooth wear. Continuous tooth eruption >1 mm over the 16 y was found in 4 of 140 adults and averaged to 0.33 mm, equaling 0.021 mm/y. In the total sample, an increase in continuous eruption was significantly associated with decreases in mean gingival height ( B = −0.34; 95% CI, −0.65 to −0.03). In a subsample of participants without tooth loss, continuous eruption was negatively associated with PD. This study confirmed that continuous eruption is clearly detectable and may contribute to lower gingival heights in the maxilla.

Supplemental material, sj-pdf-1-jdr-10.1177_0022034521999363 for Is Continuous Eruption Related to Periodontal Changes? A 16-Year Follow-up by C. Wiedemann, C. Pink, A. Daboul, S. Samietz, H. Völzke, E. Schulz-Kornas, K.F. Krey, B. Holtfreter and T. Kocher in Journal of Dental Research

Self-thinning dynamics are often considered when managing stand density in forests and are used to constrain forest growth models. However, self-thinning relationships are often quantified using only data at a conceptualised self-thinning line, even though self-thinning can begin before the stand actually reaches a self-thinning line. Also, few self-thinning relationships account for the effects of species composition in mixed-species forests, and stand structure such as relative height of species (in mixtures), and/or size or age cohorts in uneven-aged forests. Such considerations may be important given the effects of global climate change and interest in mixed-species and uneven-aged forests. The objective of this study was to develop self-thinning relationships based on changes in the tree density relative to mean tree diameter, instead of focusing only on data for state variables (e.g. tree density) at the self-thinning line. This was done while also considering how the change in tree density is influenced by site quality and stand structure (species composition and relative height). The relationships were modelled using data from temperate Australian Eucalyptus plantations (436 plots), subtropical forests in China (88 plots), and temperate forests in Switzerland (1055 plots). Zero-inflated and hurdle generalized linear models with Poisson and negative binomial distributions were fit for several species, as well as for all-species equations. The intercepts and slopes of the self-thinning lines were higher than many published studies which may have resulted from both the less restrictive equation form and data selection. The rates of self-thinning often decreased as the proportion of the object species increased, as relative height increased (species or size cohort became more dominant), and as site (quality) index increased. The effects of aridity varied between species, with self-thinning increasing with aridity index for Abies alba, Pinus sylvestris, Quercus petraea and Quercus robur, but decreasing with aridity index for Eucalyptus nitens, Fagus sylvatica and Picea abies as sites became wetter and cooler. Self-thinning model parameters were not correlated with species traits, including specific leaf area, wood basic density or crown diameter – stem diameter allometry. All-species self-thinning relationships based on all data could be adjusted using a correction factor for rarer species where there were insufficient data to develop species-specific equations. The approach and equations developed could be used in forest growth models to calculate how the tree density declines as mean tree size increases, as height changes relative to other cohorts or species, as species proportions change, and as climatic and edaphic conditions change.

The cost of harvesting short-rotation plantation eucalypts can be in excess of AU$2500·ha−1. Despite this high cost, the extent to which harvesting productivity is affected by tree genetics is not well understood. We address this issue in a study of two 10-year-old genetic field trials of Eucalyptus globulus Labill. in Australia. Standing-tree traits analysed were survival, diameter at breast height, basal area, and stem straightness and forking. Harvest traits analysed were volume, time, and productivity. Genetic group and within-group genetic variation (additive and dominance), stand-level family variation, phenotypic and genetic correlations, and the effects of inbreeding were estimated for these traits. The different scenarios studied showed that plantation harvest productivity was affected by tree genetics to some degree but was mainly affected through positive covariation with stem diameter. Harvest productivity is thus unlikely to have been adversely affected by past selection. Although no significant additive or dominance genetic variation in stem forking or straightness was detected, weak phenotypic correlations were consistent with harvest productivity being higher in straighter trees with no forking. High inbreeding depression was evident for growth and survival; however, in open-pollinated progeny, this resulted in only a slight reduction in harvest productivity (5.5%) compared with out-crossed progeny.

The impact of the manipulation of plantation stocking density on individual tree size can affect final harvest costs and machine productivity. This paper investigated the impact of four early-age thinning treatments applied to a Eucalyptus globulus Labill. pulplog plantation in south-west Western Australia on economic profitability and harvester productivity. Eighteen sample plots were randomly laid out in the study area. The nominal 700, 500, and 400 stems per hectare (sph) plots were thinned to waste 3.2 years after establishment while the nominal 1000 sph (UTH) plots were left unthinned. The economic analysis showed that all thinning treatments resulted in a lower Land Expectation Value (LEV) and net financial loss over the full rotation at their theoretical optimal rotation age when compared with the unthinned control treatment. Tree growth and form were positively impacted by thinning. However, associated reductions in harvesting costs were less than the value losses resulting from reduced per hectare yield.

The choice of planting density is a primary silvicultural decision in plantation management which considers the trade-off between individual tree size and total stand production, affecting the type, quantity and quality of products throughout the rotation. Trends in size and production with planting density are generally well known, however, less so is the interacting effect of site quality. Consequently, a case study in which basal area and basal area growth of Eucalyptus globulus Labill. plantations on five site qualities (122–435 m3·ha−1) planted at six densities (625 trees·ha−1, 4 m × 4 m; 833 trees·ha−1, 3 m × 4 m; 1000 trees·ha−1, 4 m × 2.5 m; 1250 trees·ha−1, 4 m × 2 m; 1667 trees·ha−1, 3 m × 2 m; and 2000 trees·ha−1, 3 m × 1.75 m) were used to investigate this interaction. As expected, both mean tree diameter of the whole stand and the basal area of the largest diameter 200 trees·ha−1 (D200 trees) were higher at lower planting densities, whereas whole stand basal area was greater at higher planting densities. However, there were no significant (P > 0.32) interactions between planting density and site quality for D200 or stand basal area, which contrasts with thinning responses in similar stands. This simplifies management considerations and suggests that trials at a given site quality may provide useful information about responses to planting density at other site qualities for the studied species.

Assisted reproductive technology (ART) is considered an important tool in the conservation of endangered species, but often the most limiting factor of ART is the availability of mature oocytes. The aim of the present study was to investigate the feasibility of preserving female germ cells from ovaries of female lions (Panthera leo). Good quality cumulus-oocyte complexes (COCs) were isolated and subjected to in vitro maturation (IVM). In addition, ovarian cortex was obtained and cut into pieces for culture and cryopreservation by slow freezing. The survival of ovarian follicles was assessed by histology. Frozen-thawed samples of ovarian cortex samples were xenotransplanted under the skin of ovariectomized immunodeficient mouse for 28 days. Overall, 178 intact COCs were obtained from 13 lions, but only 28.1% were matured in vitro indicating insufficient IVM conditions. In contrast, almost all follicles within the ovarian cortex survived culture when the original sample was from a young healthy lion collected immediately after euthanasia. Within the xenotransplants, the number of primordial follicles decreased after 28 days by 20%, but the relation between primordial and growing follicles changed in favour of follicular growth. Female gamete rescue from valuable felids may be performed by slow freeze cryopreservation of ovarian cortex. Although the IVM protocol for lions is not yet optimized, mature oocytes may be obtained after long-term xenotransplantation and IVM and could potentially represent one way of salvage of endangered felid species in the future.

There is little published information on the variability of pulp yield within eucalypt stems. This is primarily due to the difficulty in measuring pulp yield in small samples, especially those obtained nondestructively. Thus, information of the effect of site, climate, and silviculture on pulp yield variability is lacking, minimizing its consideration in the economic optimization of pulpwood production. Near infrared spectroscopic measurement of pulp yield directly from intact wood surfaces at a small, radial sampling interval provides forest managers with opportunities to better understand and manage its sources of variability. Calibrations based on solid wood samples were constructed to describe radial variation in Kraft pulp yield and cellulose content using intact wood samples from Eucalyptus globulus Labill. Plantations at three sites with contrasting annual rainfall were studied. Pulp yield and cellulose content were higher at the more productive, wetter sites. Outer wood near the cambium had pulp yield values up to 8% higher than those at the pith. There was no clear intra-annual cycle of variation. The more productive sites exhibited significantly steeper pith-to-bark increases in pulp yield than the driest site. This has implications for optimizing pulp productivity by considering rotation length and site quality.

Australia is at an early stage of exploring the use of forest biomass to generate energy. This study evaluated the biomass yield and the productivity rates of equipment for harvesting biomass in a poor-quality eucalypt plantation. The operation consisted of a tracked feller-buncher, grapple skidder and mobile chipper. Time study methods were used to measure the harvesting operation. A multiple regression model was constructed to predict skidding productivity. Biomass production was 63.9 t ha−1 based on the recorded load weight of eight trucks. Delays were documented and analysed. The average delay for all equipment was about 30% of working time. The study results will help guide biomass harvesting managers to estimate productivity and cost of similar operation sites.

The ability to freeze unfertilized oocytes and the ovarian cortex is valuable for genetic resource banking, especially in the case of genetically important individuals that undergo castration or euthanasia for medical reasons. Although offspring have been produced from frozen–thawed oocytes in mice, humans, cattle and rabbits, the results have been variable and not sufficiently successful for routine use. This review provides an overview on the recent knowledge of female germ cell cryopreservation, with a focus on wildlife species, and introduces the Felid Gametes Rescue Project within European Association of Zoos and Aquaria zoos.

On January 20, 2007, the Transparenzrichtlinie-Umsetzungsgesetz (Transparency Directive Implementation Act or TUG) came into effect implementing the European Transparency Directive. It entails some significant changes to the disclosure requirements of German listed corporations, such as a newly introduced Bilanzeid (confirmation of the balance sheet). In addition, material alterations to the share ownership notification rules in the Wertpapierhandelsgesetz (German Securities Trading Act or WpHG) have been made. The latter changes should be carefully observed by investors and are analyzed in this article.

The mass composition of high-energy cosmic rays at energies above 1015 eV can provide crucial information for the understanding of their origin. Air showers were measured simultaneously with the SPASE-2 air shower array and the AMANDA-B10 Cherenkov telescope at the South Pole. This combination has the advantage to sample almost all high-energy shower muons and is thus a new approach to the determination of the cosmic ray composition. The change in the cosmic ray mass composition was measured versus existing data from direct measurements at low energies. Our data show an increase of the mean log atomic mass 〈lnA〉 by about 0.8 between 500 TeV and 5 PeV. This trend of an increasing mass through the "knee" region is robust against a variety of systematic effects. © 2004 Elsevier B.V. All rights reserved.

This paper presents the use of inductively coupled plasma MS (ICP-MS) for direct elemental analysis in oleochemicals (long-chain fatty acid derivative). It focuses on Ca, P, and Fe, which are key elements for many industrial applications and which are known to be challenging for MS technology. The instrument is operated with a “shield torch” interface under “high-power cool plasma.” In addition, oxygen is blended with argon to prevent buildup of carbon on both torch and cones. The extra sensitivity of the ICP-MS technology permits high dilution factors, thereby minimizing any potential matrix effects. The contents of Ca, P, and Fe are quantified by standard addition. There is no need to resort to interference corrections for P, because it is measured as its oxide PO+. Detection limits lie in the sub-ppm for Ca and P and in the low-ppb for Fe. Accuracy of the method has been demonstrated for our products by a recovery test.

The Antarctic Muon And Neutrino Detector Array (AMANDA) is a high-energy neutrino telescope operating at the geographic South Pole. It is a lattice of photo-multiplier tubes buried deep in the polar ice between 1500m and 2000m. The primary goal of this detector is to discover astrophysical sources of high energy neutrinos. A high-energy muon neutrino coming through the earth from the Northern Hemisphere can be identified by the secondary muon moving upward through the detector. The muon tracks are reconstructed with a maximum likelihood method. It models the arrival times and amplitudes of Cherenkov photons registered by the photo-multipliers. This paper describes the different methods of reconstruction, which have been successfully implemented within AMANDA. Strategies for optimizing the reconstruction performance and rejecting background are presented. For a typical analysis procedure the direction of tracks are reconstructed with about 2 degree accuracy., 40 pages, 16 Postscript figures, uses elsart.sty

We report on the analysis of air showers observed in coincidence by the Antarctic Muon and Neutrino detector array (AMANDA-B10) and the South Pole Air Shower Experiment (SPASE-1 and SPASE-2). We discuss the use of coincident events for calibration and survey of the deep AMANDA detector as well as the response of AMANDA to muon bundles. This analysis uses data taken during 1997 when both SPASE-1 and SPASE-2 were in operation to provide a stereo view of AMANDA. © 2003 Elsevier B.V. All rights reserved.

The IceCube neutrino telescope, to be constructed near the Antarctic South Pole, represents the next generation of neutrino telescope. Its large 1 km3 size will make it uniquely sensitive to the detection of neutrinos from astrophysical sources. The current design of the detector is presented. The basic performance of the detector and its ability to search for neutrinos from various astrophysical sources has been studied using detailed simulations and is discussed.

We present results from the AMANDA high energy neutrino telescope located at the South Pole. They include measurements of the atmospheric neutrino flux, search for UHE point sources, and diffuse sources producing electromagnetic/hadronic showers at the detector or close to it.

We show new results from both the older and newer incarnations of AMANDA (AMANDA-B10 and AMANDA-II, respectively). These results demonstrate that AMANDA is a functioning, multipurpose detector with significant physics and astrophysics reach. They include a new higher-statistics measurement of the atmospheric muon neutrino flux and preliminary results from searches for a variety of sources of ultrahigh energy neutrinos: generic point sources, gamma-ray bursters and diffuse sources producing muons in the detector, and diffuse sources producing electromagnetic or hadronic showers in or near the detector.

This paper briefly describes the principle of operation and science goals of the AMANDA high energy neutrino telescope located at the South Pole, Antarctica. Results from an earlier phase of the telescope, called AMANDA-BIO, demonstrate both reliable operation and the broad astrophysical reach of this device, which includes searches for a variety of sources of ultrahigh energy neutrinos: generic point sources, Gamma-Ray Bursts and diffuse sources. The predicted sensitivity and angular resolution of the telescope were confirmed by studies of atmospheric muon and neutrino backgrounds. We also report on the status of the analysis from AMANDA-II, a larger version with far greater capabilities. At this stage of analysis, details of the ice properties and other systematic uncertainties of the AMANDA-II telescope are under study, but we have made progress toward critical science objectives. In particular, we present the first preliminary flux limits from AMANDA-II on the search for continuous emission from astrophysical point sources, and report on the search for correlated neutrino emission from Gamma Ray Bursts detected by BATSE before decommissioning in May 2000. During the next two years, we expect to exploit the full potential of AMANDA-II with the installation of a new data acquisition system that records full waveforms from the in-ice optical sensors.

Background Diffusion weighted MRI (MR-DWI) is frequently used in oncologic therapy monitoring as it is known to predict therapy response much earlier, than measurements of tumour size alone. In several studies the outcome of chemotherapeutic treatment of brain, soft tissue, bone, breast and prostate tumours was predicted by changes in MR-DWI. Usually, follow-up studies 2 to 4 weeks after induction of chemotherapy show an increase in the apparent diffusion coefficient (ADC), predicting size changes at the end the treatment cycle. DWI measurements in patients with NSCLC ultra early after starting chemotherapy are missing.

Transmission (400 to 2500 nm) and reflectance spectra (400 to 2200 nm) were recorded from polyethylene films (low-density PE, cryst. 60%, additive free), with a thickness ranging from 0.2 to 8.46 mm. The information depth, defined as the thickness of material that results in the loss of 50% of the light measured as reference, was calculated for both reflectance and transmission geometries. The resulting curves clearly show PE characteristics. For transmission, the information depth varies between ∼100 μm (at 2300 nm) and 830 μm (at 1600 nm). For reflectance, the information depth varies between 0 and 2.5 mm. The reflectance geometry proves to be the better way of measuring through a PE film. The calculated information curve also shows that at PE bands of low to medium strength the contribution of the bulk is very influential. The greater the strength of the PE band, the lower the contribution of the bulk to the spectrum. In case of an inhomogeneous sample, the information depth will reflect the effect of the inhomogeneity on repeated measurements; the weaker the band, the smaller the effect of inhomogeneity will be.

We report on the analysis of air showers observed in coincidence by the Antarctic Muon and Neutrino detector array (AMANDA-B10) and the South Pole Air Shower Experiment (SPASE-1 and SPASE-2). We discuss the use of coincident events for calibration and survey of the deep AMANDA detector as well as the response of AMANDA to muon bundles. This analysis uses data taken during 1997 when both SPASE-1 and SPASE-2 were in operation to provide a stereo view of AMANDA.

Permeabilized bovine adrenal chromaffin cells have been used to characterize the MgATP requirement of processes preceding exocytosis. Incubation of primary cultures with the membrane-permeable phenylarsine oxide (PAO) at 20 microM inhibited the phosphorylation of phosphatidylinositol (PtdIns) and completely blocked secretion. This block could be reversed by addition of 2,3-dimercaptopropanol to the permeabilized cells. Simultaneous addition of [gamma32P]ATP and 2,3-dimercaptopropanol permitted a comparison between recovery of secretion and phosphorylation of intracellular components. Recovery of secretion closely correlated with phosphorylation of PtdIns and PtdIns4P. Subcellular fractionation of permeabilized cells after recovery of secretion revealed that the majority of newly phosphorylated PtdIns4P was localized on the chromaffin granules. In accordance with these results, PtdIns 4-kinase activity was found in protein extracts of permeabilized cells as well as associated with purified chromaffin granules, sensitive in both cases to PAO. Additionally, PtdIns 4-kinase activity in these two assays was inhibited by quercetin. In permeabilized cells, quercetin decreased the levels of labeled PtdIns4P and Ptdlns(4,5)P2 and inhibited secretion. Our data suggest that a chromaffin granule-associated PtdIns 4-kinase acts in the priming of exocytosis.

We present new data taken with the AMANDA-II neutrino telescope array. The AMANDA-II upgrade was completed at the beginning of 2000. It significantly extends the sensitivity of the 10-string AMANDA-B10 detector to high- and ultrahigh-energy neutrino fluxes into regions of interest for probing current astrophysical models which remain unexplored by other experiments.

Extracellular matrix (ECM) molecules extracted from the leech central nervous system (CNS) provide substrates that induce extensive growth of processes of identified leech nerve cells in culture. Two ECM molecules, laminin and tenascin, have been identified. The laminin-like molecule has been purified and shown to be a cross-shaped molecule similar to vertebrate laminin with subunits of 340, 220, 180, and 160 kD. Purified laminin as a substrate induces rapid outgrowth of Retzius (R) and Anterior Pagoda (AP) cells in culture. The tenascin molecule has been partially purified. In electronmicrographs, leech tenascin, like vertebrate tenascin, has six arms of equal size joined in a central globule. Highly enriched fractions of leech tenascin induce rapid and extensive outgrowth of Retzius and AP cells in culture. Substrate molecules not only induce outgrowth of processes but also affect the growth patterns of individual nerve cells. Neurites are straight with few branches in laminin, but curved with profuse branches on tenascin. During regeneration of the CNS in the animal, laminin appears at new sites associated with growth cones. The appearance of laminin correlates with the accumulation of microglial cells. Thus, ECM molecules with growth-promoting activity for leech nerve cells in vitro appear to be involved in inducing regeneration and allowing the neurites to reconnect with former targets. © 1992 John Wiley & Sons, Inc.

IceCube is a km-scale neutrino observatory under construction at the South Pole with sensors both in the deep ice (InIce) and on the surface (IceTop). The sensors, called Digital Optical Modules (DOMs), detect, digitize and timestamp the signals from optical Cherenkov-radiation photons. The DOM Main Board (MB) data acquisition subsystem is connected to the central DAQ in the IceCube Laboratory (ICL) by a single twisted copper wire-pair and transmits packetized data on demand. Time calibration is maintained throughout the array by regular transmission to the DOMs of precisely timed analog signals, synchronized to a central GPS-disciplined clock. The design goals and consequent features, functional capabilities, and initial performance of the DOM MB, and the operation of a combined array of DOMs as a system, are described here. Experience with the first InIce strings and the IceTop stations indicates that the system design and performance goals have been achieved., 42 pages, 20 figures, submitted to Nuclear Instruments and Methods A

A search for TeV-PeV muon neutrinos with AMANDA-II data collected between 2000 and 2003 established an upper limit of E2Φ90%C:L

A search for TeV - PeV muon neutrinos from unresolved sources was performed on AMANDA-II data collected between 2000 and 2003 with an equivalent livetime of 807 days. This diffuse analysis sought to find an extraterrestrial neutrino flux from sources with non-thermal components. The signal is expected to have a harder spectrum than the atmospheric muon and neutrino backgrounds. Since no excess of events was seen in the data over the expected background, an upper limit of E^{2}\Phi_{90% C.L.} < 7.4 x 10^{-8} GeV cm^{-2} s^{-1} sr^{-1} is placed on the diffuse flux of muon neutrinos with a \Phi \propto E^{-2} spectrum in the energy range 16 TeV to 2.5 PeV. This is currently the most sensitive \Phi \propto E^{-2} diffuse astrophysical neutrino limit. We also set upper limits for astrophysical and prompt neutrino models, all of which have spectra different than \Phi \propto E^{-2}., Comment: 47 pages, 13 figures. This includes the original document and an erratum for the effective area tables

The description of the space debris environment and its sources in Chapter 2 reflects a widely accepted, common understanding among space debris researchers. There are, however, different methods in existence for reproducing the observed environment by means of mathematical and physical models of release processes, for propagating orbits of release products, and for mapping the propagated environment onto spatial and temporal distributions of object densities, transient velocities, and impact fluxes. The subsequent chapters will focus on methods which have been developed at ESA, or under ESA contracts in context with ESA’s MASTER-2001 model (Meteoroid and Space Debris Terrestrial Environment Reference, (Bendisch et al., 2002)). At the end of this chapter a general overview of some of the most prominent space debris environment models will be provided.

This paper deals with automatic road extraction from airborne SAR imagery. Automatic extracted road networks are often incomplete. Even sophisticated road extraction approaches that are developed for optical imagery often fail to extract a complete road network. In SAR imagery in contrary to optical imagery, roads are more affected by high backscattering context objects. These local context objects like trees, bridges, or vehicles can disturb road extraction, but they can also support it. We show that an explicit modeling of context objects, leads to improved extraction results.

We present the results of a search for point sources of high energy neutrinos in the northern hemisphere using AMANDA-II data collected in the year 2000. Included are flux limits on several AGN blazars, microquasars, magnetars and other candidate neutrino sources. A search for excesses above a random background of cosmic-ray-induced atmospheric neutrinos and misreconstructed downgoing cosmic-ray muons reveals no statistically significant neutrino point sources. We show that AMANDA-II has achieved the sensitivity required to probe known TeV gamma-ray sources such as the blazar Markarian 501 in its 1997 flaring state at a level where neutrino and gamma-ray fluxes are equal., Comment: 5 pages, 5 figures, 1 table, submitted to PRL

We present the results of a Monte-Carlo study of the sensitivity of the planned IceCube detector to predicted fluxes of muon neutrinos at TeV to PeV energies. A complete simulation of the detector and data analysis is used to study the detector's capability to search for muon neutrinos from sources such as active galaxies and gamma-ray bursts. We study the effective area and the angular resolution of the detector as a function of muon energy and angle of incidence. We present detailed calculations of the sensitivity of the detector to both diffuse and pointlike neutrino emissions, including an assessment of the sensitivity to neutrinos detected in coincidence with gamma-ray burst observations. After three years of datataking, IceCube will have been able to detect a point source flux of E^2*dN/dE = 7*10^-9 cm^-2s^-1GeV at a 5-sigma significance, or, in the absence of a signal, place a 90% c.l. limit at a level E^2*dN/dE = 2*10^-9 cm^-2s^-1GeV. A diffuse E-2 flux would be detectable at a minimum strength of E^2*dN/dE = 1*10^-8 cm^-2s^-1sr^-1GeV. A gamma-ray burst model following the formulation of Waxman and Bahcall would result in a 5-sigma effect after the observation of 200 bursts in coincidence with satellite observations of the gamma-rays., Comment: 33 pages, 13 figures, 6 tables

This paper briefly describes the principle of operation and science goals of the AMANDA high energy neutrino telescope located at the South Pole, Antarctica. Results from an earlier phase of the telescope, called AMANDA-B10, demonstrate both reliable operation and the broad astrophysical reach of this device, which includes searches for a variety of sources of ultrahigh energy neutrinos: generic point sources, Gamma-Ray Bursts and diffuse sources. The predicted sensitivity and angular resolution of the telescope were confirmed by studies of atmospheric muon and neutrino backgrounds. We also report on the status of the analysis from AMANDA-II, a larger version with far greater capabilities. At this stage of analysis, details of the ice properties and other systematic uncertainties of the AMANDA-II telescope are under study, but we have made progress toward critical science objectives. In particular, we present the first preliminary flux limits from AMANDA-II on the search for continuous emission from astrophysical point sources, and report on the search for correlated neutrino emission from Gamma Ray Bursts detected by BATSE before decommissioning in May 2000. During the next two years, we expect to exploit the full potential of AMANDA-II with the installation of a new data acquisition system that records full waveforms from the in-ice optical sensors., 14 pages, 14 figures, Slightly updated from manuscript submitted to SPIE Conference (Hawaii, 2002). Sky plot and preliminary limits given for point sources using AMANDA-II. First report on GRB search using AMANDA-II. Updated acknowledgements, references and table entries

We propose a scheme for road extraction in rural areas that integrates three different modules with specific strengths. The first module employs local grouping and uses multiple scales and contest to reliably extract most parts of the road network. To connect these parts, the second module exploits the network characteristics of roads for global grouping. The third module completes the network based on an analysis of path lengths within and between connected components of the network. An evaluation of the results shows that the system benefits from the integration of different types of knowledge within the road extraction scheme.

The species of flea infesting pets and hedgehogs in Germany were investigated through a survey of small animal practitioners throughout the country who were asked to collect specimens at their veterinary practices. A total of 625 veterinarians/veterinary practices responded and provided 2445 intact anti identifiable flea specimens. These fleas originated from 294 dogs (795 fleas), 334 cats (1152 fleas), 76 hedgehogs (481 fleas), five domestic rabbits (10 fleas), one golden hamster (four fleas) and one ferret (three fleas). Dogs were found to be infested with Archaeopsylla erinacei, Chaetopsylla globiceps, Ctenocephalides canis, Ctenocephalides felis, Hystrichopsylla talpae, Nosopsyllus fasciatus, Paraceras melis and Pulex irritans. From cats, Archaeopsylla erinacei, Ceratophyllus gallinae, Ceratophyllus garei, Ctenocephalides felis, Ctenophthalmus assimilis, Hystrichopsylla talpae, Monopsyllus sciurorum, Nosopsyllus fasciatus, Spilopsyllus cuniculi and Typhloceras poppei were collected. In both dogs and cats the most prevalent species were Ctenocephalides felis (78.9% and 91.6%, respectively) and Archaeopsylla erinacei (21.1% and 12.6%, respectively) followed by Ctenocephalides canis in dogs (5.8%) and Hystrichopsylla talpae in cats (1.2%). The fleas isolated from rabbits were Ctenocephalides felis, Hystrichopsylla talpae and Spilopsyllus cuniculi. Nosopsyllus fasciatus and Ctenocephalides felis were recovered from the golden hamster and the ferret, respectively. The hedgehogs were found to be infested with Archaeopsylla erinacei, Ceratophyllus gallinae and Ctenocephalides felis.

Consideration of the course of everyday actions during which real objects are manipulated would suggest that the sequence of individual activities should be represented in the memory not as a chain but as a network with specific characteristics. These characteristics would appear to indicate that activities which directly change parts of the environment are more memorable than shifts of attention during the performance of the action. The results of Experiment 1 support this view. Furthermore, it may be supposed that complex actions are not anticipated in their entirety before being performed, but only partially. Hypotheses concerning the temporal order of the parts of the anticipation process were tested in Experiment 2, the results supporting the hypotheses. Altogether, the results obtained confirm certain basic assumptions relating to the memory model Net of Recallable Action.

The efficacy and safety of a vaccination during anaesthesia was examined in 20 puppies from 8 litters of one kennel. The animals were vaccinated in a state of reflex-free anaesthesia (neuroleptanalgesia in combination with halothane, nitrous oxide and oxygen). 20 puppies from the same litters were used as controls. A parvovirus living vaccine (Canimed) and a rabies vaccine from inactivated viruses (Rabisin) were used for the vaccinations. The titers of neutralizing antibodies against rabies virus were significantly lower in the group of anaesthetized animals compared to the control group on the 10th and 20th day p.vacc. The average titers of antibodies against parvovirus of both groups, however, do not allow any statistically significant statements. It is nevertheless remarkable that 6 of the anaesthetized puppies (three different litters) showed no increase in antibodies. The results of the investigations lead to the recommendation not to vaccinate anaesthetized puppies. The risk of reducing the efficacy of vaccinations with vaccines from inactivated agents is greater than with living vaccines.