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The purpose of this study is to reveal whether the excess workload plays a moderator role in the relationship between the role ambiguity of the employees and their perceptions of work alienation. For this purpose, the data obtained through questionnaires from 312 people working in universities, banks, health institutions and different institutions that produce security services operating in Ankara were analyzed. The scales used in the first works in the analyzes are exact analytic factor analysis as AMOS and can be simply redesigned to be able to play a simple analysis game to predict the choice among the choices and test the possible outcomes to be solved. Regression analysis based on Bootstrap was performed with the software. As a result of the findings, it was revealed that the role ambiguity perceptions of the employees affected the level of work alienation significantly and positively, and the perception of overwork had a moderating effect on the relationship between role ambiguity and work alienation. [ABSTRACT FROM AUTHOR]