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The aim of the experiment was to investigate the effect of VC and bacterial enzyme complex combined with glucose on serum biochemical indexes, immune and antioxidant function of cows with antibiotic poisoning. Twenty cows with antibiotic poisoning, weighing about (250.00±10.23) kg were randomly divided into a control group and an experimental group, with five replicates in each group and two cows in each replicate. The cows in the control group received an intravenous injection of glucose and VC, and the cows in the experimental group received an oral administration of glucose and bacterial enzyme complex for seven days. The results showed that compared with the control group, on the 8th day, the leukocytes, neutrophils, erythrocytes and hemoglobin of cows in the experimental group were significantly decreased ( P<0.05), and the platelets were significantly increased ( P<0.05). The activitiy of ALT and CK, and the concentrations of TBIL, Na, P and BUN in serum significantly decreased ( P<0.05), while the concentrations of ALB and Ca significantly increased ( P<0.05). The concentrations of IgM, IgG, IL-6, IFN-γ, SOD activity and T-AOC in the serum of the cows in the experimental group significantly increased ( P<0.05), while the concentrations of MDA significantly decreased ( P<0.05). On the 8th day of the experiment, there were no significant differences in blood routine, serum biochemistry, antioxidant indexes and immune between the experimental group and healthy cows ( P>0.05). The study indicates that glucose and bacteriase complex treatment can effectively improve the blood routine, serum biochemical, immunity and antioxidant function. [ABSTRACT FROM AUTHOR]