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26. List of Contributors

Author

Abia, Rocio, primary, Aguila, Marcia Barbosa, additional, Alcaraz, Miguel, additional, Aldini, Giancarlo, additional, Alonso, Alvaro, additional, de Cienfuegos, Gerardo Álvarez, additional, Amaral, Joana S., additional, Amirante, Paolo, additional, Amores, Victoria, additional, Amoruso, Angela, additional, Andrade, Paula B., additional, Andreadou, I., additional, Angioni, Alberto, additional, Ankan, Aysun, additional, Antunes, Lusânia Maria Greggi, additional, Apetrei, C., additional, Aramendía, María A., additional, Arbones-Mainar, Jose Miguel, additional, Arias, José L., additional, Arienti, Giuseppe, additional, Armenta, Sergio, additional, Assy, Nimmer, additional, Audi, Nama’a, additional, Azadmard-Damirchi, Sodeif, additional, Azqueta, Amaia, additional, Badiu, Diana, additional, Ballabio, Davide, additional, Balesteros, Manoela Ruchiga, additional, Ballesteros, Evaristo, additional, Barbosa, Décio Sabbatini, additional, Barbouti, Alexandra, additional, Bardelli, Claudio, additional, Batanero, Eva, additional, Baumann, Leslie, additional, Benavente-García, Obdulio, additional, Becker, Peter, additional, Bendini, Alessandra, additional, Benedetti, Simona, additional, Beretta, Giangiacomo, additional, Bermudez, Beatriz, additional, Bes-Rastrollo, Maira, additional, Bianchi, Maria de Lourdes Pires, additional, Bisignano, Giuseppe, additional, Blanco, Santos, additional, Boskou, George, additional, Bracci, Tania, additional, Bravo, Laura, additional, Brenes, Manuel, additional, Brescia, Maria Antonietta, additional, Briasoulis, Evangelos, additional, Brunelleschi, Sandra, additional, Bucci, Remo, additional, Buckland, Genevieve, additional, Buratti, Susanna, additional, Buróníó, María I., additional, Busconi, Matteo, additional, Cabello-Moruno, Rosana, additional, Caboni, Pierluigi, additional, Cabras, Paolo, additional, Caggia, Cinzia, additional, Camuesco, Desiree, additional, Cancelas, Jesús, additional, Capasso, Giovambattista, additional, Capote, F. Priego, additional, Cárdenas, Soledad, additional, Carluccio, Maria Annunziata, additional, Carrasco-Pancorbo, Alegria, additional, Carnuccio, Rosa, additional, Carrera, María Pilar, additional, Carretero, Antonio Segura, additional, Casado, Francisco Javier, additional, Castillo, Julián, additional, Caturla, Nuria, additional, Cerretani, Lorenzo, additional, Chillemi, Rosa, additional, Chiou, Antonia, additional, Choi, Shin-Geon, additional, Christian, Mildred S., additional, Chondrogianni, Niki, additional, Churruca, Itziar, additional, Cirugeda, Miguel de la Guardia, additional, de Castro, Antonio, additional, De Caterina, Raffaele, additional, Cavaliere, Chiara, additional, Chinou, Ioanna, additional, Clodoveo, Maria Lisa, additional, Coimbra, Manuel A., additional, Conte, Lanfranco S., additional, Cosio, M. Stella, additional, Covas, Maria Isabel, additional, Coxam, Véronique, additional, Cunha, Sara C., additional, Curiel, Jose Antonio, additional, D’Angelo, Stefania, additional, Daniel, Hannelore, additional, Davicco, Marie-Jeanne, additional, De Bonis, Maria Luigia, additional, De Castro, M.D. Luque, additional, de la Higuera, Manuel, additional, de la Puerta, Cristina, additional, de las Rivas, Blanca, additional, de la Torre, Rafael, additional, Delcuratolo, Debora, additional, De Leonardis, Antonella, additional, De Lucia, Maria, additional, de Oliveira, Marcone Augusto Leal, additional, de Pablo, Manuel A., additional, De Roos, Baukje, additional, De Saja, Jose A., additional, De Stefano, Daniela, additional, Di Bella, Giuseppa, additional, Dichi, Isaias, additional, Diener, Robert M., additional, Dirsch, Verena M., additional, d’Ischia, Marco, additional, Driss, Fathi, additional, Dugo, Giacomo, additional, Dutta, Paresh C., additional, Ebizuk, Yutaka, additional, El-Benna, Jamel, additional, Esteban, Francisco J., additional, Estepa, Amparo, additional, da Silva, Joaquim C.G. Esteves, additional, Fabiani, Roberto, additional, Facino, Roberto Maffei, additional, Faria, M.A., additional, Faria, Miguel, additional, Fernandes, José O., additional, Fernandez-Gutierrez, Alberto, additional, Fernández-Montesinos, Rafael, additional, Fernández-Navarro, Mónica, additional, Fernández-Quintela, Alfredo, additional, Faria, Adriana Ferreira, additional, Fitó, Montserrat, additional, Fogher, Corrado, additional, Foglia, Patrizia, additional, Franconi, Flavia, additional, Fregapane, Giuseppe, additional, Fujimoto, Kazuma, additional, Fujise, Takehiro, additional, Furneri, Pio Maria, additional, Gallardo, Visitación, additional, Galletti, Patrizia, additional, Galvez, Julio, additional, Garcia, Aranzazu, additional, García, I.M., additional, García-Luna, Pedro P., additional, Garrido-Fernández, Antonio, additional, Garrigues, Salvador, additional, Geelen, Math J.H., additional, Geladopoulos, Taxiarchis, additional, Giamarellos-Bourboulis, Evangelos J., additional, Frenich, Antonia Garrido, additional, Galaris, Dimitrios, additional, Gilani, Anwarul Hassan, additional, Gill, Chris I.R., additional, Giovannini, Claudio, additional, Glatzle, Jörg, additional, Gnoni, Gabriele V., additional, Gomes, Tommaso, additional, Gómez-Rico, Aurora, additional, Gonos, Efstathios S., additional, Gonzalez, Carlos A., additional, Goya, Luis, additional, Grosovski, Maria, additional, Gutiérrez, Guillermo Rodriguez, additional, Hartmann, Marie-Andrée, additional, Hashim, Yumi Z., additional, Hu, Xiuli, additional, Ieri, Francesca, additional, Iliodromitis, Efstathios K., additional, Iwakiri, Ryuichi, additional, Juan, M. Emilia, additional, Kalogeropoulos, Nick, additional, Kalua, Curtis, additional, Khan, Arif-ullah, additional, Khymenets, Olha, additional, Kosti, Rena, additional, Kotsiou, Antonia, additional, Kremastinos, Dimitrios Th., additional, Lafont, Fernando, additional, Laganà, Aldo, additional, Landete, Jose Maria, additional, La Pera, Lara, additional, Lapucci, Chiara, additional, Lavermicocca, Paola, additional, Léger, Claude Louis, additional, Lehotay, Steven J., additional, Leone, Alessandro, additional, Liu, Jun, additional, Turco, Vincenzo Lo, additional, Lopez, Sergio, additional, de Cerain, Adela Lopez, additional, de Felipe, Felix Lopez, additional, López-Feria, Silvia, additional, de la Serrana, Herminia López-Garcia, additional, López-López, Antonio, additional, López-Martinez, Maria Carmen, additional, Lopez-Miranda, Jose, additional, La Torre, Giovanna Loredana, additional, Lucas, Ricardo, additional, Lupiáñez, José A., additional, Luque, Rafael, additional, Macciola, Vincenzo, additional, Madar, Zecharia, additional, Mafra, Isabel, additional, Magrì, Andrea D., additional, Magrì, Antonio L., additional, Maiuri, Maria Chiara, additional, Malaisse, Willy J., additional, Mañas, Mariano, additional, Mandarim-de-Lacerda, Carlos Alberto, additional, Manna, Caterina, additional, Mannina, Luisa, additional, Mannino, Saverio, additional, Marinas, Alberto, additional, Marinas, Jose M., additional, Marini, Federico, additional, Markaki, Panagiota, additional, Martín, M. Angeles, additional, Martínez, María Alba, additional, Martinez-Gonzalez, Miguel A., additional, Martínez-Gracia, María Victoria, additional, Martinez-Martos, José Manuel, additional, Martínez-Victoria, Emilio, additional, Masella, Roberta, additional, Massaro, Marika, additional, Mastovska, Katerina, additional, Mateos, Raquel, additional, Mazzoni, Michela, additional, Menéndez-Menéndez, Javier, additional, Medina, Eduardo, additional, Medina, Jose M., additional, Micol, Vicente, additional, Mikros, Emmanuel, additional, Mitjavila, Maria Teresa, additional, Montaño, Alfredo, additional, Morales, Juan Carlos, additional, Morcillo, Sonsoles, additional, Moreno, Juan José, additional, Moreno, Paola, additional, Moret, Sabrina, additional, Moros, Javier, additional, Morozzi, Guido, additional, Motilva, Maria-Jose, additional, Muñoz, Rosario, additional, Muriana, Francisco J.G., additional, Napoli, Daniela, additional, Napolitano, Alessandra, additional, Nassar, Faris, additional, Nasti, Raffaella, additional, Natali, Francesco, additional, Natalini, Paolo, additional, Nunes, Cláudia S., additional, Nychas, George, additional, Oliveira, M. Beatriz P.P., additional, Orihara, Yutaka, additional, Ortega, Almudena, additional, Ortega-García, Francisca, additional, Osada, Jesús, additional, Özdemir, Durmuş, additional, Öztürk, Betül, additional, Pacheco, Yolanda M., additional, Paice, Alistair G., additional, Palmerini, Carlo A., additional, Panagiotakos, Demosthenes, additional, Panagou, E.Z., additional, Panzella, Lucia, additional, Papoti, V.T., additional, Paradiso, Vito Michele, additional, Pariente, José Antonio, additional, Patel, Vinood B., additional, Peinado, M. Ángeles, additional, Pellicanò, Teresa Maria, additional, Peragon, Juan, additional, Perez-Jimenez, Francisco, additional, Perez-Martinez, Pablo, additional, Perona, Javier Sanchez, additional, Piperno, Anna, additional, Planas, Joana M., additional, Pollicino, Donatella, additional, Polzonetti, Valeria, additional, Populin, Tiziana, additional, Portillo, Maria P., additional, Pozo, David, additional, Prenzler, Paul, additional, Prieto, Pablo G., additional, Pucciarelli, Stefania, additional, Puel, Caroline, additional, Puertollano, Elena, additional, Puertollano, María A., additional, Quesada-Granados, Jose Javier, additional, Rajendram, Rajkumar, additional, Ramírez-Expósito, María Jesús, additional, Ramos, Sonia, additional, Ramos-Martos, Natividad, additional, Randazzo, Cinzia L., additional, Rando, Rossana, additional, Reiter, Russel J., additional, Rejano, Luis, additional, Rhee, Hae-Ik, additional, Robards, Kevin, additional, Rodriguez, Hector, additional, Rodríguez-Cabezas, Maria Elena, additional, Rodríguez-Manero, Moises, additional, Rodríguez-Méndez, María L., additional, Rodríguez, Rosalía, additional, Rodriguez-Rodriguez, Rosalia, additional, Romani, Annalisa, additional, Romero, Concepción, additional, Romero, Maria-Paz, additional, Romero-Gonzalez, Roberto, additional, Rose, Martin, additional, Rossi, Mauro, additional, Rotondi, Annalisa, additional, Rowland, Ian R., additional, Ruiz, Adolfina, additional, Ruiz-Gutiérrez, Valentina, additional, Russo, Francesco, additional, Ryan, Danielle, additional, Sabatini, Nadia, additional, Sacco, Antonio, additional, Sahan, Yasemin, additional, Saitta, Marcello, additional, Salvador, Maria Desamparados, additional, Samperi, Roberto, additional, Samaniego-Sánchez, Cristina, additional, Sánchez, Antonio Higinio, additional, Sánchez-Navarro, Maria Rosa, additional, Sancho, Verónica, additional, Santos-González, Mónica, additional, Saraiva, Jorge A., additional, Scampicchio, Matteo, additional, Scazzocchio, Beatrice, additional, Schmitt, Christoph A., additional, Schwartz, Betty, additional, Sciuto, Sebastiano, additional, Scoditti, Egeria, additional, Seabra, Rosa M., additional, Sebastiani, Luca, additional, Segre, Anna Laura, additional, Segura, Antonio, additional, Servili, Maurizio, additional, Shiraishi, Ryosuke, additional, Siculella, Luisa, additional, Simão, Andréa Colado, additional, Sindona, Giovanni, additional, Sivakumar, Ganapathy, additional, Skaltsounis, Alexios-Leandros, additional, Smith, Amos B., additional, Spatafora, Carmela, additional, Sperry, Jeffrey B., additional, Soares, Mario J., additional, Soriguer, Federico, additional, Srirajaskanthan, Rajaventhan, additional, Stitia, Naïm, additional, Tabernero, Arantxa, additional, Tagarelli, Antonio, additional, Tai, Fuju, additional, Tamborrino, Antonia, additional, Tassou, C.C., additional, Tesseromatis, Christine, additional, Todeschini, Roberto, additional, Torrecilla, Jose S., additional, Trikib, Saïda, additional, Tringali, Corrado, additional, Tsakiris, Ioannis N., additional, Tsatsakis, Aristidis M., additional, Tsimidou, M.Z., additional, Uccella, Nicola A., additional, Urbano, Francisco J., additional, Valcarcel, Miguel, additional, Valencia-Serrano, Félix, additional, Valentão, Patrícia, additional, Valverde, Isabel, additional, Varela, Lourdes M., additional, Varì, Rosaria, additional, Simas Vaz, Fernando Antonio, additional, Vettorazzi, Ariane, additional, Vichi, Stefania, additional, Vidal, José Luis Martínez, additional, Villalba, José M., additional, Villalba, Mayte, additional, Villanueva-Peñacarrillo, María L., additional, Vincenzetti, Silvia, additional, Vita, Alberto, additional, Vlahov, Giovanna, additional, Wang, Wei, additional, Weisberg, Edmund, additional, Wenzel, Uwe, additional, Won, Se-Ra, additional, Wu, Bin, additional, Yago, María Dolores, additional, Zabaras, Dimitrios, additional, Zappia, Vincenzo, additional, Zarzuelo, Antonio, additional, and Zhang, Luyong, additional

Published
2010
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37. Simvastatin inhibits the expression of stemness-related genes and the metastatic invasion of human cancer cells via destruction of the cytoskeleton

Author

Tatè, Rosarita, primary, Zona, Enrica, additional, De Cicco, Rosanna, additional, Trotta, Veronica, additional, Urciuoli, Maria, additional, Morelli, Alessandro, additional, Baiano, Salvatore, additional, Carnuccio, Rosa, additional, Fuggetta, Maria Pia, additional, and Morelli, Franco, additional

Published
2017
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39. BIOACTIVE PHYSICAL CHITOSAN-BASED GELS FOR WOUND HEALING

Author

CAMPANI, VIRGINIA, MAYOL, LAURA, CARNUCCIO, ROSA, MAIURI, MARIA CHIARA, DE ROSA, GIUSEPPE, Daniela De Stefano, Ilaria Bressani, Eleonora Ferrari, Claudia Cencetti, Giuseppina Acerra, Luigi Maiuri, Pietro Matricardi, Campani, Virginia, Mayol, Laura, Daniela De, Stefano, Ilaria, Bressani, Eleonora, Ferrari, Claudia, Cencetti, Carnuccio, Rosa, Giuseppina, Acerra, Luigi, Maiuri, Pietro, Matricardi, Maiuri, MARIA CHIARA, and DE ROSA, Giuseppe

Published
2013

41. A decoy oligonucleotide to NF-κB delivered through inhalable particles inhibits the lung inflammation induced by LPS in rat

Author

Daniela De Stefano, Ciro Coletta, Lucia Falcone, Ivana d'Angelo, D'EMMANUELE DI VILLA BIANCA, ROBERTA, UNGARO, FRANCESCA, QUAGLIA, FABIANA, CARNUCCIO, ROSA, SORRENTINO, RAFFAELLA, SIF, Daniela De, Stefano, Ciro, Coletta, D'EMMANUELE DI VILLA BIANCA, Roberta, Lucia, Falcone, Ivana, D'Angelo, Ungaro, Francesca, Quaglia, Fabiana, Carnuccio, Rosa, and Sorrentino, Raffaella

Abstract

NF-kB plays a prominent role in orchestrating the airway inflammatory response in several diseases, including cystic fibrosis [1]. The specific inhibition of NF-kB by decoy oligonucleotides delivered in the lung may limit the progression of inflammation [2], although rationally designed systems are needed to control drug release and optimize pharmacological response. In this regard, here we have developed and tested in vivo an inhalable dry powder for prolonged delivery of a decoy oligodeoxynucleotide to NF-κB (dec-ODN), consisting of large porous particles (LPP) based on poly(lactic-coglycolic) acid (PLGA). First, LPP containing dec-ODN (dec-ODN LPP) have been engineered to meet aerodynamic criteria crucial for pulmonary delivery, to gain an effective loading of dec-ODN, to sustain its release and to preserve its structural integrity in lung lining fluids. Then, we have investigated the effects of dec-ODN LPP in a rat model of lung inflammation induced by intra-tracheal aerosolization of LSP from P. aeruginosa. The results show that a single intratracheal insufflation of dec-ODN LPP significantly prevented the neutrophil infiltration induced by LPS up to 72 hours, whereas naked dec-ODN was able to inhibit it only at 6 hours. The persistent inhibition of neutrophil infiltrate by dec-ODN LPP was associated with a significant reduction of NF-κB/DNA binding activity as well as interleukin-6, interleukin-8 and mucin-2 mRNA expression in lung homogenates. Taken together, our findings show that dec-ODN LPP may provide a new strategy for local treatment of inflammation associated with lung diseases. 1.Park GY and Christman JW. Nuclear factor kappa B is a promising therapeutic target in inflammatory lung disease. Curr Drug Targets. 2006 Jun;7(6):661-8. 2.Cabrini G et al., Targeting transcription factor activity as a strategy to inhibit pro-inflammatory genes involved in cystic fibrosis: decoy oligonucleotides and low-molecular weight compounds. Curr Med Chem. 2010;17(35):4392-404.

Published
2013

42. The role of NF-kB in chronic inflammation

Author

CARNUCCIO, ROSA, MAIURI, MARIA CHIARA, De Stefano D., Wei-Chiao Chang, Wen-Chang Chang, Carnuccio, Rosa, Maiuri, MARIA CHIARA, and De Stefano, D.

Published
2013

44. Development of new physical gels based on chitosan for wound healing

Author

V. Campani, I. Bressani, E. Ferrari, G. Acerra, L. Maiuri, MAYOL, LAURA, DE STEFANO, DANIELA, CARNUCCIO, ROSA, MAIURI, MARIA CHIARA, DE ROSA, GIUSEPPE, APGI, V., Campani, Mayol, Laura, DE STEFANO, Daniela, I., Bressani, E., Ferrari, Carnuccio, Rosa, G., Acerra, L., Maiuri, Maiuri, MARIA CHIARA, and DE ROSA, Giuseppe

Abstract

Purpose: To investigate the potential of new chitosan-based physical gels, alone or loaded with plant extract or growth factors, for wound healing. Methods: Chitosan (CHI) was autoclaved and then dissolved at 2% w/w in acetic acid 0.1 M; the resulting dispersion was sonicated and then placed under vacuum for 48h. CHI gels were differently loaded depending of the considered active. In particular, gels loaded with PDGF and FGF-?? were prepared by co-dissolution of the growth factors with CHI in acetic acid 0.1 M. Alternatively, Centella asiatica (CEN) or Echinacea angustifolia (ECH) were added to the preformed gel. Thermal and rheological analysis were carried out to highlight the differences due to the heat treatment on the viscoelastic properties of gels. In addition, the effects of the gels on human fetal foreskin fibroblasts (HFFF-2) viability, proliferation and migration were investigated. Finally, the effect of the different gels on wound healing was investigated on C57BL/6J mice using an experimental model of pressure ulcer. Results: The differential scanning calorimeter (DSC) analysis showed that, the thermal treatment modifies the chemical and physical characteristics of the CHI powder. Furthermore, the formulations with non-autoclaved CHI showed a rheological behavior typical of an entangled network, while gels prepared from the autoclaved CHI have a typical gel behavior. The loading of actives into the gel did not affect the rheological properties of the formulations. In vitro tests on HFFF-2 showed that CHI gels were not toxic and led to increased cell proliferation and migration. Finally, the presence of PDGF ,FGF-??, CEN or ECH in the gels shows also to enhance the migration, the proliferation without affecting the vitality of fibroblasts. In vivo, CHI gel enhanced wound healing compared to untreated mice. This effect was higher when using CHI gels containing PDGF, FGF-??, CEN or ECH. Studies are in progress to determine the gel with the highest effect on wound healing, as well as the mechanism driving the tissue regeneration. Conclusions: The rheological properties of the developed CHI gels as well as in vitro and in vivo results, demonstrated a high potential as new products for the medication of wound, i.e. pressure ulcers.

Published
2012

45. Sviluppo di nuovi gel fisici a base di chitosano per il trattamento di piaghe da decubito

Author

V. Campani, I. Bressani, E. Ferrari, G. Acerra, L. Maiuri, MAYOL, LAURA, DE STEFANO, DANIELA, CARNUCCIO, ROSA, MAIURI, MARIA CHIARA, DE ROSA, GIUSEPPE, ADRITELF, V., Campani, Mayol, Laura, DE STEFANO, Daniela, I., Bressani, E., Ferrari, Carnuccio, Rosa, G., Acerra, L., Maiuri, Maiuri, MARIA CHIARA, and DE ROSA, Giuseppe

Abstract

L???obiettivo di questo lavoro è stato lo sviluppo e la caratterizzazione di nuovi gel fisici sterili a base di chitosano, solo o in associazione a estratti vegetali, quali Centella asiatica (CEN) ed Echinacea angustifolia (ECH), e/o fattori di crescita, quali PDGF e FGF-????????? per il trattamento di piaghe da decubito. Per tutte le formulazioni sono stati determinate le proprietà viscoelastiche e termiche, mediante analisi reologica e Calorimetria a Scansione Differenziale (DSC). Infine è stato saggiato l???effetto delle diverse formulazioni su fibroblasti umani di prepuzio (HFFF-2) e sono stati effettuati studi in vivo su modelli sperimentali di piaghe da decubito indotte in topi C57BL/6J. L???analisi DSC ha evidenziato che la sterilizzazione in autoclave della polvere di CHI ne modifica le caratteristiche fisico-chimiche e ne cambia il comportamento reologico, che diventa quello tipico di un gel. Le sostanze attive caricate nei gel non influenzano in maniera significativa le proprietà reologiche delle formulazioni. Gli studi condotti su cellule HFFF-2 hanno evidenziato che i gel a base di CHI non sono tossici e inducono proliferazione e migrazione cellulare. L???impiego di sostanze attive caricate nei gel comporta un significativo aumento della migrazione e della proliferazione, soprattutto quando in miscela tra loro. Gli studi in vivo hanno confermato che i gel a base di CHI, soprattutto quando caricati con sostanze attive, aumentano la velocità di rigenerazione di tessuti lesi.

Published
2012

47. Transferosomes and ethosomes for the trans-resveratrol permeation through the skin: a comparative study

Author

SCOGNAMIGLIO, IMMACOLATA, DE STEFANO, DANIELA, CARNUCCIO, ROSA, LA ROTONDA, MARIA IMMACOLATA, DE ROSA, GIUSEPPE, Phospholipids Institute, Scognamiglio, Immacolata, DE STEFANO, Daniela, Carnuccio, Rosa, LA ROTONDA, MARIA IMMACOLATA, and DE ROSA, Giuseppe

Abstract

Introduction: The trans-resveratrol (t-res) is a non flavonoid polyphenol with promising properties for the treatment or prevention of different diseases. In particular, topical application of resveratrol could be advantageous in the prevention of skin cancer or in the treatment of inflammation-based diseases such as psoriasis. However, different studies demonstrated the importance of the formulation to enhance t-res delivery into or trough the skin. Nanocarriers, such as ethosomes and transferosomes, have been successfully used to deliver drugs with different characteristics into or through the skin. Purpose: The aim of the present work was to develop a nanocarrier-based formulations for skin delivery of t-res. Methods: Ethosomes or transferosomes containing t-res were prepared by a modified hand-shaking method followed by extrusion or sonication. All the formulations were characterised in terms of mean diameter, size distribution (I.P.), t-res loading, t-res stability upon encapsulation during storage at 4°C. The inhibition of reactive oxygen species (ROS) and lipids peroxidation in human keratinocyte (HaCaT) cell line stimulated with H2O2 for 24 h and then incubated with the t-res containing nanocarriers was investigated. Moreover, for all the formulations, ex vivo permeation studies on porcine skin was carried out by means of Franz diffusion cells. At appropriate intervals (0.5, 1, 2, 4, 6, 24 h), an aliquots of the receptor medium were withdrawn and the t-res concentration was determined by HPLC. Results: All the t-res containing carriers were characterised by a very high (between 70 to 100%) encapsulation efficiency, a limited t-res release at 4°C during the storage and stability of resveratrol in its trans form. Nanocarriers encapsulating t-res reduced, in a concentration-dependent manner, ROS production induced by H2O2, with the higher effect observed in the case of ethosomes. Permeation studies showed that only ethosomes were able to promote t-res permeation through porcine skin and this effect was affected by lipid composition of the carrier. Conclusions: In this work, transferosomes showed the highest encapsulation efficiency and the lowest t-res release. On the other hand, only ethosomes were able to promote t-res permeation through the skin.

Published
2011

48. Biodegradable particles for local and prolonged delivery of an oligonucleotide decoy to nuclear factor-kB in the lung

Author

UNGARO, FRANCESCA, DE STEFANO, DANIELA, GIOVINO, CONCETTA, D'EMMANUELE DI VILLA BIANCA, ROBERTA, SORRENTINO, RAFFAELLA, CARNUCCIO, ROSA, QUAGLIA, FABIANA, Coletta C., Polimeno A., Dalby R.N., Byron P.R., Peart J., Suman J.D., Young P.M., Ungaro, Francesca, DE STEFANO, Daniela, Giovino, Concetta, Coletta, C., Polimeno, A., D'EMMANUELE DI VILLA BIANCA, Roberta, Sorrentino, Raffaella, Carnuccio, Rosa, and Quaglia, Fabiana

Subjects
oligonucleotide, cystic fibrosis, respirable dry powder, PLGA
Abstract

NF-kB blockade by decoy oligonucleotides (ODNs) able to interfere with NF-kB transcriptional activity may be of great help in limiting the progression of CF lung chronic inflammation. Although data proving the validity of this approach are just emerging, early studies on CF airway epithelial cell lines are encouraging. Major concerns arise from the need of appropriate systems for ODN inhalation, that are able to efficiently target the desired region of the lungs, protect the ODN in the biological environment and release it in a sustained manner. The technological approaches currently adopted for lung delivery of ODNs reliy on the use of lipidic nanoparticles or cationic liposomes, providing new formulation options for dispersed liquid droplet dosage forms (i.e., bulky, expensive and more time-consuming nebulizers) with poor control over ODN release rate. Prompted by this, we are currently developing respirable dry powders, for prolonged pulmonary delivery of a decoy ODN to NF-kB (dec-ODN), consisting of biodegradable large porous particles (LPP) based on poly(lactic-co-glycolic) acid (PLGA). The effects of dec-ODN LPP on NF-kB/DNA binding activity as well as IL-6 and IL-8 mRNA levels were studied on DF508 CFTR-mutated human bronchial epithelial cells stimulated with LPS from P. aeruginosa for 24 and 72 h. Finally, the effects of intratracheally administered naked dec-ODN or dec-ODN LPP on neutrophil lung recruitment induced by LPS from P. aeruginosa at 6, 24 and 72 h were investigated in a rat model of airway inflammation.

Published
2011

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