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2 results on '"CELPHEDIA infrastructure"'

1. Improving laboratory animal genetic reporting: LAG-R guidelines

Author

Lydia Teboul, James Amos-Landgraf, Fernando J. Benavides, Marie-Christine Birling, Steve D. M. Brown, Elizabeth Bryda, Rosie Bunton-Stasyshyn, Hsian-Jean Chin, Martina Crispo, Fabien Delerue, Michael Dobbie, Craig L. Franklin, Ernst-Martin Fuchtbauer, Xiang Gao, Christelle Golzio, Rebecca Haffner, Yann Hérault, Martin Hrabe de Angelis, Kevin C. Kent Lloyd, Terry R. Magnuson, Lluis Montoliu, Stephen A. Murray, Ki-Hoan Nam, Lauryl M. J. Nutter, Eric Pailhoux, Fernando Pardo Manuel de Villena, Kevin Peterson, Laura Reinholdt, Radislav Sedlacek, Je Kyung Seong, Toshihiko Shiroishi, Cynthia Smith, Toru Takeo, Louise Tinsley, Jean-Luc Vilotte, Søren Warming, Sara Wells, C. Bruce Whitelaw, Atsushi Yoshiki, Asian Mouse Mutagenesis Resource Association, CELPHEDIA infrastructure, INFRAFRONTIER consortium, International Mammalian Genome Society, International Mouse Phenotyping Consortium, International Society for Transgenic Technologies, Mutant Mouse Resource and Research Centers, Phenomics Australia, RRRC- Rat Resource and Research Center, and Guillaume Pavlovic

Subjects
Science
Abstract

Abstract The biomedical research community addresses reproducibility challenges in animal studies through standardized nomenclature, improved experimental design, transparent reporting, data sharing, and centralized repositories. The ARRIVE guidelines outline documentation standards for laboratory animals in experiments, but genetic information is often incomplete. To remedy this, we propose the Laboratory Animal Genetic Reporting (LAG-R) framework. LAG-R aims to document animals’ genetic makeup in scientific publications, providing essential details for replication and appropriate model use. While verifying complete genetic compositions may be impractical, better reporting and validation efforts enhance reliability of research. LAG-R standardization will bolster reproducibility, peer review, and overall scientific rigor.

Published
2024
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2. Fetal estrogens are not involved in sex determination but critical for early ovarian differentiation in rabbits

Author

Eloïse Airaud, Aurélie Dewaele, Laurent Boulanger, Nathalie Daniel-Carlier, Frank Giton, Beatrice Mandon-Pepin, Maëlle Pannetier, Geneviève Jolivet, Cloé Pierson, Eric Pailhoux, Erwana Harscoët, Nathalie Daniel, Biologie de la Reproduction, Environnement, Epigénétique & Développement (BREED), École nationale vétérinaire d'Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École nationale vétérinaire - Alfort (ENVA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), ANR-09-GENM-0009,GENIDOV,Réseaux géniques impliqués dans la différenciation ovarienne chez la chèvre et la souris(2009), ANR-13-BSV2-0017,ARGONADS,Acide rétinoique et devenir des cellules germinales(2013), IMRB - 'Biologie du système neuromusculaire' [Créteil] (U955 Inserm - UPEC), École nationale vétérinaire - Alfort (ENVA)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, This study was supported by ANR grants (GENIDOV:ANR-09-GENM-009, ARGONADS: ANR-13-BSV2-0017, ARDIGERM: ANR-2020-CE14), and the CELPHEDIA infrastructure (CELPHEDIA France, 2017)., ANR-20-CE14-0022,ARDIGERM,Acide rétinoïque dans la différenciation des cellules germinales et la méiose(2020), Jolivet, Geneviève, Génomique et biotechnologies végétales - Réseaux géniques impliqués dans la différenciation ovarienne chez la chèvre et la souris - - GENIDOV2009 - ANR-09-GENM-0009 - GENOM-BTV - VALID, Blanc 2013 - Acide rétinoique et devenir des cellules germinales - - ARGONADS2013 - ANR-13-BSV2-0017 - Blanc 2013 - VALID, and Acide rétinoïque dans la différenciation des cellules germinales et la méiose - - ARDIGERM2020 - ANR-20-CE14-0022 - AAPG2020 - VALID

Subjects
Anti-Mullerian Hormone, Sex Differentiation, Somatic cell, [SDV]Life Sciences [q-bio], sex determination, AROMATASE, 0302 clinical medicine, INDEL Mutation, Ovarian Follicle, TALEN, Testosterone, Aromatase, Antrum, [SDV.BDD]Life Sciences [q-bio]/Development Biology, media_common, 0303 health sciences, 030219 obstetrics & reproductive medicine, Estradiol, biology, Gene Expression Regulation, Developmental, Cell Differentiation, Phenotype, medicine.anatomical_structure, Female, Cytochrome P450 Family 19, Rabbits, AcademicSubjects/MED00250, Research Article, Ovulation, endocrine system, Gonad, media_common.quotation_subject, rabbit, Andrology, 03 medical and health sciences, [SDV.BDD] Life Sciences [q-bio]/Development Biology, medicine, Animals, CYP19A1, Gonads, Ovarian reserve, Cell Proliferation, 030304 developmental biology, Fetus, Sexual differentiation, Gene Expression Profiling, Estrogens, Sex Determination Processes, CYP26A1, biology.protein, ovary
Abstract

AROMATASE, encoded by the CYP19A1 gene, is the cytochrome enzyme responsible for the synthesis of estrogens in vertebrates. In most mammals a peak of expression of the CYP19A1 gene occurs in the fetal XX gonad when sexual differentiation starts up. To elucidate the role of this peak, we produced 3 lines of TALEN genetically edited CYP19A1 KO rabbits, that were void of any production of estradiol. All KO XX rabbits developed as females, with aberrantly small sized ovaries at adulthood, an almost empty reserve of primordial follicles and very few large antrum follicles. Ovulation never occurred. Our histological, immunohistological and transcriptomic analyses showed that the surge of estradiol in the XX fetal rabbit gonad is dispensable for its determination as an ovary, or for meiosis. However, it is mandatory for the high proliferation and differentiation of both somatic and germ cells, and consequently for the establishment of the ovarian reserve.

Published
2021
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