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Parental downward support plays an important role in urban and rural sustainable development. It is of great significance to study parental downward transfers and their motivation. However, there is no consensus on the motivation behind parental downward transfers in China. This study examines the timing and monetary impacts of social pensions on parental downward transfers and assesses the motivations behind them. We found that pension insurance encouraged rural parents to provide time and monetary support to their children. Unlike rural parents, pension insurance increased the monetary support of urban parents but inhibited their time support. Because of the higher income level of urban parents and the better organization of the domestic service market, parents have the motivation and conditions to reduce their time support and increase monetary support. Our findings highlight the importance of parental downward transfers in urban and rural sustainable development.

Objective To investigate the effect of the SMAC gene on paclitaxel sensitivity and cellular activity in lung adenocarcinoma cells based on the caspase-3/Bcl-2/Bax signaling pathway. Methods A paclitaxel-resistant cell line A549/Taxol was established for lung adenocarcinoma, and the cells were divided into four following groups: pcDNA-NC (transfected with pcDNA-NC blank vector), pcDNA-SMAC (transfected with pcDNA-SMAC vector), siRNA-NC (transfected with siRNA-NC empty virus vector), and siRNA-SMAC groups (transfected with siRNA-SMAC lentiviral vector). The SMAC mRNA expression in cells was detected by qRT-PCR; cell sensitivity was detected by MTT; cell proliferation ability was detected by cloning assay; cell invasion ability was detected by Transwell; apoptosis ability was detected by flow cytometry assay; and caspase-3, Bcl-2 and Bax protein expression in cells were detected by Western blot analysis. Results The SMAC mRNA expression was significantly lower in A549 cells compared with BEAS-2B cells (P < 0.05). The SMAC mRNA expression was significantly higher in the pcDNA-SMAC group than that in the pcDNA-NC group cells (P < 0.05). The SMAC mRNA expression was significantly lower in the cells of the siRNA-SMAC group (P < 0.05) than that in the siRNA-NC group. The SMAC mRNA expression was significantly lower in the cells of the siRNA-SMAC group (P < 0.05) than in the siRNA-NC group. Compared with the pcDNA-NC group, the cell IC50, cell clone number, cell invasion ability, and Bcl-2 protein and Bcl-2/Bax ratio were significantly lower in the pcDNA-SMAC group, the cell resistance index reversal was 2.51-fold, and the apoptosis ability and caspase-3, as well as Bax protein expression, were significantly higher (P < 0.05). Compared with the siRNA-NC group, cell IC50, cell clone number, cell invasion ability, and Bcl-2 protein and Bcl-2/Bax ratio were significantly higher in the siRNA-SMAC group, and apoptosis ability and caspase-3 and Bax protein expression were significantly lower (P < 0.05). Conclusion High expression of SMAC increases paclitaxel sensitivity, inhibits cell growth and invasion, promotes apoptosis in lung adenocarcinoma cells, and has a regulatory effect on the caspase-3/Bcl-2/Bax signaling pathway.

Transcription factor AP-2γ regulates not only the genetic procedures that control the proliferation and differentiation of trophoblast cells during early embryonic development, but also is the key target in the development of breast cancer, lung cancer, testicular cancer, melanoma and pancreatic cancer, thus affecting the occurrence and development of tumors. Therefore, paying attention to the regulatory mechanisms of AP-2γ in the occurrence and development of malignant tumors may provide new ideas for the diagnosis and treatment of tumors.

Objective To explore the influence of negative lymph node count (NLNC) on the prognosis of patients with gastric signet ring cell carcinoma (GSRC) and develop a prognostic nomogram based on NLNC. Methods On the basis of the SEER database, 2 101 patients diagnosed with GSRC were collected and randomly divided into the modeling group and validation group to test the relationship between clinicopathological characteristics and the prognosis of GSRC. The multivariate Cox proportional hazard regression model was used to analyze the independent risk factors affecting overall survival and establish a prognostic prediction model. The consistency index (C-index), calibration curve, net reclassification index (NRI), integrated discrimination improvement (IDI), and decision curve analysis (DCA) were used to evaluate the accuracy and clinical applicability of the nomogram. Results All patients were divided according to the ratio of 7:3, with 1 473 in the modeling group and 628 in the validation group. NLNC > 10 (HR=0.578, 95%CI: 0.504-0.662, P < 0.001) was a protective factor for the prognosis of patients with GSRC, and the nomogram model was established based on multivariate Cox proportional hazards model. The C-index values of the nomogram were 0.737 (95%CI: 0.720-0.753) and 0.724 (95%CI: 0.699-0.749) in the modeling and validation groups, respectively, showing good discrimination. The calibration curves showed high consistency of the model. NRI=17.77%, continuous NRI=36.34%, and IDI=4.2% indicated that the model had positive returns compared with the traditional model. The DCA was far from the baseline, indicating that the model had good clinical applicability. Conclusion The increase in NLNC is a favorable factor for the prognosis of patients with GSRC, and a relatively accurate nomogram was established to predict the prognosis of patients with GSRC and help clinicians conduct individualized prognostic evaluations.

BackgroundIt is reported that insulin resistance widely exists in non-diabetic patients with a recent history of transient ischemic attack (TIA) or ischemic stroke. There is currently strong evidence to prove the bidirectional effect of glucose metabolism disorders and stroke events. Therefore, it is necessary to retrospectively tease out the current status, hotspots, and frontiers of insulin resistance and ischemic cerebrovascular disease through CiteSpace.Materials and methodsWe searched the Web of Science (WOS) for studies related to insulin resistance and ischemic cerebrovascular disease from 1999 to April 2022, then downloaded the data into CiteSpace to generate a knowledge visualization map.ResultsA total of 1,500 publications relevant to insulin resistance and ischemic cerebrovascular disease were retrieved. The USA had the most articles on this topic, followed by PEOPLES R CHINA and JAPAN. WALTER N KERNAN was the most prolific author, whose research mainly focused on insulin resistance intervention after stroke (IRIS) trial. The most common keywords were myocardial ischemia, metabolic syndrome, ischemic stroke, cerebral ischemia, association, oxidative stress, inflammation, and adipose tissue. Major ongoing research trends include three aspects: (1) the association between insulin resistance and ischemic cerebrovascular disease in non-diabetic patients, (2) the intrinsic pathological mechanism between insulin resistance and ischemic cerebrovascular disease, and (3) early intervention of insulin resistance to improve the prognosis of stroke.ConclusionThe results of this bibliometric study provide the current status and trends of clinical research publications in the field of insulin resistance and ischemic cerebrovascular disease. Insulin resistance is strongly associated with the occurrence of ischemic stroke, early neurological deterioration in stroke patients, post-stroke depression, and cerebral small vessel disease. Early treatment of insulin resistance can be an effective way to prevent the onset of ischemic stroke and improve stroke prognosis. This study may help researchers to identify hot topics and explore new research directions.

Type 2 diabetes is associated with insulin resistance, impaired insulin secretion and liver steatosis. Here the authors report a proof-of-concept study for small molecule SWELL1 modulators as a therapeutic approach to treat diabetes and associated liver steatosis by enhancing systemic insulin-sensitivity and insulin secretion in mice.

Spatially resolved transcriptomics (SRT) is capable of comprehensively characterizing gene expression patterns and providing an unbiased image of spatial composition. To fully understand the organizational complexity and tumor immune escape mechanism, we propose stMGATF, a multiview graph attention fusion model that integrates gene expression, histological images, spatial location, and gene association. To better extract information, stMGATF exploits SimCLRv2 for visual feature exaction and employs edge feature enhanced graph attention networks for the learning potential embedding of each view. A global attention mechanism is used to adaptively integrate 3 views to obtain low-dimensional representation. Applied to diverse SRT datasets, stMGATF is robust and outperforms other methods in detecting spatial domains and denoising data even with different resolutions and platforms. In particular, stMGATF contributes to the elucidation of tissue heterogeneity and extraction of 3-dimensional expression domains. Importantly, considering the associations between genes in tumors, stMGATF can identify the spatial dark genes ignored by traditional methods, which can be used to predict tumor-driving transcription factors and reveal tumor immune escape mechanisms, providing theoretical evidence for the development of new immunotherapeutic strategies.

Non-small cell lung cancer (NSCLC) is a malignant tumor with the highest mortality rate in our country. It has been found in many studies that microRNA-4521 (miR-4521) is abnormally expressed and plays a role in clear cell renal cell carcinoma and other cancers.

Osimertinib is a third-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) and a star medication used to treat non-small-cell lung carcinomas (NSCLCs). It has caused broad public concern that osimertinib has relatively low stability in plasma. We explored why osimertinib and its primary metabolites AZ-5104 and AZ-7550 are unstable in rat plasma. Our results suggested that it is the main reason inducing their unstable phenomenon that the Michael addition reaction was putatively produced between the Michael acceptor of osimertinib and the cysteine in the plasma matrix. Consequently, we identified a method to stabilize osimertinib and its metabolite contents in plasma. The assay was observed to enhance the stability of osimertinib, AZ-5104, and AZ-7550 significantly. The validated method was subsequently applied to perform the pharmacokinetic study for osimertinib in rats with the newly established, elegant, and optimized ultra-performance liquid chromatography–tandem mass spectrometer (UPLC-MS/MS) strategy. The assay was assessed for accuracy, precision, matrix effects, recovery, and stability. This study can help understand the pharmacological effects of osimertinib and promote a solution for the similar problem of other Michael acceptor-contained third-generation EGFR-TKI.

Juice sac granulation is a common internal physiological disorder of citrus fruit. In the present study, we compared the physiochemical characteristics and transcriptome profiles of juice sacs in different granulation levels from Huyou fruit (Citrus changshanensis). The accumulation of cell wall components, including the water-soluble pectin, protopectin, cellulose, and lignin, were significantly correlated with the granulation process, resulting in the firmness increase of the juice sac. The in situ labeling of the cell wall components indicated the early accumulation of cellulose and high-methylesterified pectin in the outer layer cells, as well as the late accumulation of lignin in the inner layer cells of the juice sac. Several phytohormones, including auxins, abscisic acids, cytokinins, jasmonic acid, salicylic acid, and/or their metabolites, were positively correlated to the granulation level, indicating an active and complex phytohormones metabolism in the granulation process. Combining the trend analysis by the Mfuzz method and the module-trait correlation analysis by the Weighted Gene Co-expression Network Analysis method, a total of 2940 differentially expressed genes (DEGs) were found to be positively correlated with the granulation level. Gene Ontology (GO) enrichment indicated that the selected DEGs were mainly involved in the cell wall organization and biogenesis, cell wall macromolecule metabolic process, carbohydrate metabolic process, and polysaccharide metabolic process. Among these selected genes, those encoding β-1,4-xylosyltransferase IRX9, cellulose synthase, xyloglucan: xyloglucosyl transferase, xyloglucan galactosyltransferase MUR3, α-1,4-galacturonosyltransferase, expansin, polygalacturonase, pectinesterase, β-glucosidase, β-galactosidase, endo-1,3(4)-β-glucanase, endoglucanase and pectate lyase that required for the biosynthesis or structural modification of cell wall were identified. In addition, NAC, MYB, bHLH, and MADS were the top abundant transcription factors (TFs) families positively correlated with the granulation level, while the LOB was the top abundant TFs family negatively correlated with the granulation level.

Although progress has been achieved in the pharmacological activity and toxicity of Radix Polygoni Multiflori (RPM), the chemical basis of its toxicity is still unclear. Here, we performed a multicompound pharmacokinetic analysis and investigated the tissue distribution and excretion characteristics of RPM components after oral administration in rats. The findings demonstrated that the active ingredients of the RPM extract were quickly absorbed after oral administration, with high exposure levels of emodin, 2,3,5,4′-teterahydroxystilbene-2-O-β-D-glucoside (TSG), citreorosein, torachrysone-8-O-glucoside (TG), emodin-8-O-β-D-glucoside (EG), and physcion-8-O-β-D-glucoside (PG). The tissue distributions of emodin, TSG, TG, EG, and PG were high in the liver and kidney. These components were the key contributors to the effectiveness and toxicity of RPM on the liver and kidney. Most of the active ingredients were mainly excreted through feces and bile, while a few were converted into other products in the body and excreted through urine and feces.

Dyslexia is the most prevalent disorder in the world that causes difficulties with reading, writing, and spelling. Pupils with dyslexia show trouble with their cognitive skills. Various interventions were already introduced for their treatment but dyslexia is still a trending disorder. The available interventions utilized for these pupils' learning open the research area for the current state-of-art of learning interventions for pupils with dyslexia. The results of this Systematic Literature Review show the trending interventions, sensory approaches utilized, and difficulties for pupils with dyslexia learning. Papers published over a period of 5 years were reviewed and their data was collected using a rigid systematic process. Based on the gathered data, several analyses were conducted. The search shows that nowadays, technological-based interventions are trending specifically apps and games, in parallel haptics technology is in its very initial stage. The most predominant sensory approaches were visual and auditory, followed by kinesthetic and tactile, mainly intervening with non-technological and technological interventions. There are still many open issues and research opportunities in the field of learning interventions for pupils with dyslexia, as most researchers utilized the visual and auditory approaches for the feedback and guidance of these pupils, while they lack to utilize the kinesthetic and tactile.

BACKGROUND The altered wall rocks of copper deposits and associated ore bodies have a close genetic and spatial relationship. By analyzing the alteration characteristics of copper deposits, the physical and chemical conditions of the mineralization, the migration, enrichment and evolution of ore-forming elements in the hydrothermal fluid can be obtained. The alteration features ultimately indicate the mineralization degree of the copper deposit and the location of the ore body. The hyperspectral core scanning system is a new type of technical testing method, developed in recent years. This article documents the first time of studying the representative cores from the Chengmenshan Tielukan copper deposit by the hyperspectral core scanning system. At the same time, altered minerals were analyzed by electron probe microanalysis (EMPA). Thus, further revealing the metallogenic mechanism of the Chengmenshan copper deposit. OBJECTIVES To investigate the alteration features of the Chengmenshan Tielukan copper deposit and to understand the ore genesis. METHODS Samples were analyzed by a hyperspectral core scanning system and electron probe microanalyzer. RESULTS The results showed that in the typical borehole ZKJ9-7, the typical spectral curves of montmorillonite and carbonate were dominated at 0-350m, whereas the typical spectral curves of kaolin and muscovite were dominated at 350-578m. The mineral composition variation from the surface to the depth of the Tielukan region in the Chengmenshan copper deposit periphery was montmorillonite+kaolinite →carbonate+montmorillonite →carbonate →muscovite+kaolinite+montmorillonite →muscovite+kaolinite+chlorite. CONCLUSIONS In the Tielukan region, the contact zone between the granodiorite porphyry and the carbonate wall rocks controls the superficial part, but in the deep part it has alteration processes such as skarnization, silicification and chloritization. These alteration processes will conduce to the formation and enrichment of the copper mine. The deep part of the drilling core also shows that rock component exchange occurred on both sides of the contact zone, resulting in the formation of the skarn in the deep part. When the ascending mineralized solution flows along the carbonate interface, CaO in carbonate diffuse to the ferrosilicon rock and aluminosilicon rock along with the intergranular solution. In contrast, FeO, Al2O3 and SiO2 in the ferrosilicon rock and aluminosilicon rock diffuse to limestone, and thus rock components exchange on both sides of the contact zone generating the skarn in the deep part. The formation of copper-rich skarn deposit is closely related to the components exchange between solution and rock. The diffusion effect caused by the concentration difference of the components plays an important role.

Using organic solvent in traditional polymer filtration membrane is easy to cause environ-mental pollution.The silk was uniformly grafted with polyacrylonitrile using zinc chloride aqueous sol-ution as the solvent. The nano-TiO2 was blended into the mixture and the casting solution was pre-pared. The TiO2 blended silk fibroin grafted PAN (SF-g-PAN/TiO2) filtration membrane was pre-pared using water as the coagulation bath. The membrane was characterized by scanning electron micr-oscopy and infrared spectroscopy. The hydrophilic, anti-fouling and mechanical properties of SF-g-PAN membranes with different TiO2contents were studied. The rejection performance of dyes with different molecular weight by the SF-g-PAN/TiO2 membrane was investigated. The results show that the mechanical properties of the SF-g-PAN/TiO2 filtration membranes increase firstly and then dec-rease with the increase of TiO2 content. When the content of TiO2 is 1%(mass fraction), the mech-anical and hydrophilic properties of the SF-g-PAN/TiO2 membrane are the best; the rejection of the dyes whose molecular weight is more than 660 is greater than 96%, with the flux of 80 L/(m2·h) at 0.10 MPa.

Objective To express the cashew nut allergen Ana o 2 and identify its immunological activity. Methods Ana o 2 gene was synthesized and inserted into the pET-28a (+) expression vector. The recombinant plasmid pET-28a (+) - Ana o 2 was constructed and sequenced. The correct recombinant plasmid was transformed into Rosetta (DE3), and the recombinant Ana o 2 protein was expressed and purified. The purified recombinant protein was identified by mass spectrometry. The immunological activity of the recombinant Ana o 2 protein was evaluated by enzyme-linked immunosorbent assay (ELISA) and protein immunoblot (Western blot). Results As determined by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight of the recombinant protein was approximately 54 kD, which was consistent with the theoretical value. The recombinant protein was identified as Ana o 2 by mass spectrometry. ELISA result showed that the level of specific immunoglobulin E (sIgE) in cashew-allergic sera was significantly different from that in negative sera (t=2.44, P

In view of problems that existing mine positioning equipment embedded program upgrade method needs to establish physical connection and there’s no programmable wireless upgrade protocol for small embedded devices, a wireless software upgrade method for mine positioning equipments was proposed. The method adopts wireless broadcast transmission mode and one-to-many network mode to implement batch wireless IAP (In-Application Programming) upgrade of mine positioning device, thereby improving convenience and efficiency of upgrade. The experiment results show that the overall upgrade success rate of the method is positively correlated with distance, and the success rate is high within 10 m. The method provides an effective way for upgrading of a large number of mine positioning equipments.

[Objectives]Establish a method capable of quickly calculating the flow velocity of an artesian circulating system,in order to optimize and compare multiple design schemes for key components of the sea water artesian circulating system.[Methods]Numerical simulation of the sea water artesian circulating system of a ship is done by using the RANS method and the SST k-ω turbulence model. Body force is used instead of main components to do numerical analysis. A method for finding the intersection point by using system characteristic curve and component performance curve is proposed,to identify the flow velocity points of sea water artesian circulating system.[Results]By comparing the operating condition points obtained by the body force method with those obtained by the component-hull combined calculation method,the calculation accuracy of body force is verified,so as to determine the flow velocity of the sea water artesian circulating system. The body force method is more convenient and less computationally intensive than the combined calculation method,therefore the body force method is more suitable for multi-scheme simulation analysis on system components.[Conclusions]The body force method can simplify the numerical simulation calculation and provide a reference for predicting the flow velocity and flow of the artesian circulating system of the ship.

In view of the lack of effective linkage between audio communication and video communication in current mine integrated dispatching communication system, it cannot support dispatcher to proceed efficient audio and video linkage communication, a mine-used audio and video linkage software was designed. The software middleware obtains status of terminal of scheduling communication host in real time through OCP protocol, the data processing module judges the phone status, and sends the linked video information to the client through the message queue RabbitMQ. The client receives the legitimate instructions sent by the data processing module and controls the opening and closing of the video terminal.The test results show that the software is stable and easy to install; through the Web configuration management platform, users can easily manage the telephone and video information, and configure the corresponding relationship between the two;for each scheduling phone bounded by client, when the phone is calling party or called, the camera information of phone of the other party can be displayed on the screen. The video information can be displayed smoothly and the response can be made within 1 s,which meets real-time requirements of users.

The understanding of the electrophysiological mechanisms that underlie mechanosensitivity of the sinoatrial node (SAN), the primary pacemaker of the heart, has been evolving over the past century. The heart is constantly exposed to a dynamic mechanical environment; as such, the SAN has numerous canonical and emerging mechanosensitive ion channels and signaling pathways that govern its ability to respond to both fast (within second or on beat-to-beat manner) and slow (minutes) timescales. This review summarizes the effects of mechanical loading on the SAN activity and reviews putative candidates, including fast mechanoactivated channels (Piezo, TREK, and BK) and slow mechanoresponsive ion channels [including volume-regulated chloride channels and transient receptor potential (TRP)], as well as the components of mechanochemical signal transduction, which may contribute to SAN mechanosensitivity. Furthermore, we examine the structural foundation for both mechano-electrical and mechanochemical signal transduction and discuss the role of specialized membrane nanodomains, namely, caveolae, in mechanical regulation of both membrane and calcium clock components of the so-called coupled-clock pacemaker system responsible for SAN automaticity. Finally, we emphasize how these mechanically activated changes contribute to the pathophysiology of SAN dysfunction and discuss controversial areas necessitating future investigations. Though the exact mechanisms of SAN mechanosensitivity are currently unknown, identification of such components, their impact into SAN pacemaking, and pathological remodeling may provide new therapeutic targets for the treatment of SAN dysfunction and associated rhythm abnormalities.

Perilipin 2 (PLIN2) is a lipid droplet (LD) protein in β cells that increases under nutritional stress. Downregulation of PLIN2 is often sufficient to reduce LD accumulation. To determine whether PLIN2 positively or negatively affects β cell function under nutritional stress, PLIN2 was downregulated in mouse β cells, INS1 cells, and human islet cells. β Cell–specific deletion of PLIN2 in mice on a high-fat diet reduced glucose-stimulated insulin secretion (GSIS) in vivo and in vitro. Downregulation of PLIN2 in INS1 cells blunted GSIS after 24-hour incubation with 0.2 mM palmitic acid. Downregulation of PLIN2 in human pseudoislets cultured at 5.6 mM glucose impaired both phases of GSIS, indicating that PLIN2 is critical for GSIS. Downregulation of PLIN2 decreased specific OXPHOS proteins in all 3 models and reduced oxygen consumption rates in INS1 cells and mouse islets. Moreover, we found that PLIN2-deficient INS1 cells increased the distribution of a fluorescent oleic acid analog to mitochondria and showed signs of mitochondrial stress, as indicated by susceptibility to fragmentation and alterations of acyl-carnitines and glucose metabolites. Collectively, PLIN2 in β cells has an important role in preserving insulin secretion, β cell metabolism, and mitochondrial function under nutritional stress.

Contaminations frequently occur in bacterial cultures, which significantly affect the reproducibility and reliability of the results from whole-genome sequencing (WGS). Decontaminated WGS data with clean reads is the only desirable source for detecting possible variants correctly. Improvements in bioinformatics are essential to analyze the contaminated WGS dataset. Existing pipelines usually contain contamination detection, decontamination, and variant calling separately. The efficiency and results from existing pipelines fluctuate since distinctive computational models and parameters are applied. It is then promising to develop a bioinformatical tool containing functions to discriminate and remove contaminated reads and improve variant calling from clean reads. In this study, we established a Python-based pipeline named CleanSeq for automatic detection and removal of contaminating reads, analyzing possible genome variants with proper verifications via local re-alignments. The application and reproducibility are proven in either simulated, publicly available datasets or actual genome sequencing reads from our experimental evolution study in Escherichia coli. We successfully obtained decontaminated reads, called out all seven consistent mutations from the contaminated bacterial sample, and derived five colonies. Collectively, the results demonstrated that CleanSeq could effectively process the contaminated samples to achieve decontaminated reads, based on which reliable results (i.e., variant calling) could be obtained.

The endothelium responds to numerous chemical and mechanical factors in regulating vascular tone, blood pressure, and blood flow. The endothelial volume-regulated anion channel (VRAC) has been proposed to be mechanosensitive and thereby sense fluid flow and hydrostatic pressure to regulate vascular function. Here, we show that the leucine-rich repeat-containing protein 8a, LRRC8A (SWELL1), is required for VRAC in human umbilical vein endothelial cells (HUVECs). Endothelial LRRC8A regulates AKT-endothelial nitric oxide synthase (eNOS) signaling under basal, stretch, and shear-flow stimulation, forms a GRB2-Cav1-eNOS signaling complex, and is required for endothelial cell alignment to laminar shear flow. Endothelium-restricted Lrrc8a KO mice develop hypertension in response to chronic angiotensin-II infusion and exhibit impaired retinal blood flow with both diffuse and focal blood vessel narrowing in the setting of type 2 diabetes (T2D). These data demonstrate that LRRC8A regulates AKT-eNOS in endothelium and is required for maintaining vascular function, particularly in the setting of T2D.

In the present study the white-rot fungi BP was selected to study the degradation of nitrogen heterocyclic compounds in real and simulated coking wastewater. The study incorporated analysis of the degradation process, mechanism of action of white-rot fungi on nitrogen heterocyclic compounds, changes of enzyme activity and white-rot fungi biomass growth rate in coking wastewater (simulated and actual). The results showed that addition of ammonia nitrogen and phenol in simulated wastewater had insignificant effects on the degradation of indole. Moreover, the promoting effect of phenol on quinoline degradation was greater than the inhibition effects of ammonia nitrogen. Degradation rates of quinoline, indole and pyridine are consistent with the zero-order kinetics equation. It was also found that the addition of Mg (II) and Mn (II) promotes the degradation of quinoline by white-rot fungi in the actual wastewater, while degradation of pyridine was inhibited by three ions, out of which the inhibition of Cu (II) was the most obvious. Cu (II) was also found to have positive effects on the levels of activity of laccase enzyme secreted by the white-rot fungi as opposed to other metal ions tested. However, it was found to have inhibitory effects on the development of white-rot fungi, while Mg (II) and Mn (II) were found to promote the development of the white-rot fungi. The activity of the enzyme and the growth rate of biomass of white-rot fungi first reached the maximum levels followed by a significant decline.

The influence of different multi-stage aging heat treatment on microstructure and corrosion resistance of 7056 alloy was investigated by the measurements of transmission electron microscope(TEM), scanning electron microscope(SEM),mechanical properties and electrical conductivity, exfoliation corrosion, slow strain rate testing(SSRT), Tafel cyclic polarization. The results show that after over-aging and re-aging heat treatment, the solute atoms precipitate again, and the volume fraction of the precipitate in the matrix increases, the grain boundary precipitates coarsen and become discontinuous, the precipitate-free zone becomes wider. Compared with 120℃/24h pre-aging,120℃/6h has advantage in terms of dissolution of finer precipitates and the growth of large coarse phase. Re-aging heat treatment can improve the strength and conductivity of over-aging alloys;compared with peak aging and retrogression and re-aging, the loss of tensile strength of the alloy is not significant, while the conductivity increases obviously. After over-aging and re-aging heat treatment, continuous anodic dissolution is avoided effectively, and the exfoliation corrosion resistance and stress corrosion cracking is enhanced.

Using organic solvent in traditional polymer filtration membrane is easy to cause environ-mental pollution.The silk was uniformly grafted with polyacrylonitrile using zinc chloride aqueous sol-ution as the solvent. The nano-TiO2 was blended into the mixture and the casting solution was pre-pared. The TiO2 blended silk fibroin grafted PAN (SF-g-PAN/TiO2) filtration membrane was pre-pared using water as the coagulation bath. The membrane was characterized by scanning electron micr-oscopy and infrared spectroscopy. The hydrophilic, anti-fouling and mechanical properties of SF-g-PAN membranes with different TiO2contents were studied. The rejection performance of dyes with different molecular weight by the SF-g-PAN/TiO2 membrane was investigated. The results show that the mechanical properties of the SF-g-PAN/TiO2 filtration membranes increase firstly and then dec-rease with the increase of TiO2 content. When the content of TiO2 is 1%(mass fraction), the mech-anical and hydrophilic properties of the SF-g-PAN/TiO2 membrane are the best; the rejection of the dyes whose molecular weight is more than 660 is greater than 96%, with the flux of 80 L/(m2·h) at 0.10 MPa.

The effect of trace Ge on the microstructure and quenching sensitivity of 7056 aluminum alloy was studied by mechanical tensile and end quenching experiments, combined with some analysis methods such as metallographic observation, hardness test, conductivity test, scanning electron microscopy (SEM) and transmission electron microscopy (TEM).The results show that the trace element can refine the alloy grain sizes,the grain size is reduced from 137μm to 114μm; the addition of trace Ge formes coarse Mg2Ge phase on the grain boundary and inhibits the formation of the T phase.In addition, compared with the Ge-free alloy, the width of the precipitation free zone of the Ge-bearing alloy becomes narrow, and is decreased from 190nm to 90nm, and the size of the precipitated phase at the grain boundary is reduced.The trace addition of Ge could reduce the quench sensitivity of alloy, increased the quenching depth of the alloy about 25%,which may be caused by the addition of Ge which changes the number and size of η equilibrium phase during slow cooling; the addition of Ge reduces the free vacancy concentration in the alloy, non-uniform nucleation and growth of the η equilibrium phase at the defect are suppressed, which increases the number of strengthening η' precipitates after aging.Thus the drop in the strength is decreased during slow quenching,which increases the hardenability of the alloy.

In view of problems that symmetric-double-sided two-way ranging (SDS-TWR) method had low ranging efficiency and poor expansibility and there was still room for improvement in the ranging efficiency and positioning system capacity of asymmetric-double-sided two-way ranging multi-ack(ADS-TWR-MA)method in current ultra-wideband TOF underground accurate positioning technology, a method of asymmetric-double-sided two-way ranging multi-tag multi-anchor (ADS-TWR-MTMA) was proposed. On the basis of ADS-TWR-MA method, the method further multiplexes ranging message in asymmetric-double-sided two-way ranging process, so that multiple tags and multiple anchor nodes can be used for TOF ranging simultaneously. The method greatly improves ranging efficiency and capacity of positioning system under premise of ensuring ranging accuracy. The application in locomotive proximity detection system and accurate positioning system verifies that the ADS-TWR-MTMA method is superior to SDS-TWR method and ADS-TWR-MA method in number of ranging messages, ranging time and system capacity.