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Sclerotinia is a significant disease that harms the production of cruciferous crops. S. sclerotiorum can induce host to produce excessive reactive oxygen species (ROS), resulting in host cell death, and Trx plays an important role in host antioxidant process. Screening and identifying the relationship between Trx and Sclerotinia resistance will provide genetic resources for plant resistance breeding. In this study, a key gene AtTrx5 was selected from A. thaliana through bioinformatics and transcriptome data analysis. AtTrx5 has the closest relationship with Brassica napus BnaA08g04320D with 85% amino acid homology. Trx5 showed up-regulated expression in the homologous gene in rape. The lesion area of the AtTrx5 overexpression lines was reduced by 30%-39% compared with the WT, while the lesion area of T-DNA mutant was increased by about 39.2% at 24 hpi with S. sclerotiorum. The expression level of AtTrx5 in the overexpression lines was higher than wide type, while AtTrx5 was lower in the T-DNA mutant during the infection. DAB staining, H2O2 content, • O2- content indicated that the accumulation of ROS in T-DNA mutants was significantly higher than that of wide type; while the accumulation of ROS was significantly less in overexpression lines. [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinical change of post-stroke dysphagia after the intervention of Liyan Tongqiao （relieving sore-throat and dredging orifices）acupuncture using cranial diffusion tensor imaging (DTI)., Methods: A total of 60 patients with post-stroke dysphagia were enrolled and randomly divided into Liyan Tongqiao acupuncture group and neurology treatment group, with 30 patients in each group. The patients in the neurology treatment group were given routine neurology treatment and swallowing rehabilitation training, and those in the Liyan Tongqiao acupuncture group received acupuncture at Sishencong (EX-HN1), Baihui (GV20), bilateral Tai-yang (EX-HN5), and bilateral Fengchi (GB20) and tongue triple acupuncture, with an electroacupuncture apparatus for EX-HN1, bilateral GB20, and tongue triple acupuncture, for a needle retaining time of 30 minutes each time, once a day and 5 times a week, in addition to the treatment in the neurology treatment group. Each course of treatment was 3 weeks, and both groups were treated for 2 courses. Swallowing function assessment and cranial DTI were performed after treatment., Results: After 6 weeks of treatment, both groups had a marked improvement in swallowing function, a significantly greater change in video fluoroscopic swallowing study (VFSS) score and a higher mean FA value ( P <0.05). Compared with the neurology treatment group, the Liyan Tongqiao acupuncture group had a marked improvement in swallowing function, a significantly greater change in VFSS score in the pharyngeal phase and a higher mean FA value ( P <0.05)., Conclusion: Liyan Tongqiao acupuncture can improve dysphagia and swallowing function in the pharyngeal phase in VFSS, possibly by promoting the remodeling of cerebral cortex and increasing the FA value of infarct zone through the stimulation of related acupoint signals.