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The use of agricultural waste to produce value-added products has aided in managing waste management difficulties while somehow addressing cost-effectiveness concerns. The current research addresses environmental issues and helps to promote ecologically friendly agricultural practices in Punjab, Pakistan, by using banana and grapefruit peel biomass, converting them to biochar by pyrolysis, and further studying their efficacy as sustainable fertilizer. The return of biochar obtained from agricultural waste to the agricultural field is a sustainable approach for increasing the yield of crop output while reducing the environmental impact of traditional fertilizers. Furthermore, it improves soil condition by regulating pH, water holding capacity, soil organic carbon, and soil ion exchange potential. Zinc oxide loaded biochar was synthesized from banana and grapefruit peel biochar. Pyrolysis was done at 400-500°C as part of the process. Co-precipitation method was used to impregnate ZnO nanoparticles in biochar and proved an efficient and dependable approach. In-situ loading was done. XRD, elemental analysis of carbon, hydrogen, nitrogen, and sulfur (CHNS), FT-IR, and TGA methods were used to characterize synthesized materials. The adsorption ability of ZnO loaded biochar, soil, and raw biochar was investigated using several physical tests such as swelling ratio, water retention, absorbance, and equilibrium water content percentage. As a result, the adsorption capacity of ZnO loaded biochar was shown to be greater than that of raw biochar. Zinc loaded biochar was used as a fertilizer in Cicer arietinum (gram). Synthesized nano-fertilizers exhibit enhanced prolonged nutrient release, plant growth, and improved soil fertility, providing an environmentally friendly alternative to traditional fertilizers by reducing nutrient loss. [ABSTRACT FROM AUTHOR]

Chickpeas are a vital source of protein and starch for a large portion of the world's population and are known to be impacted by heat stress at every life stage. Previously known as an "Orphan Legume", little is known of the genetic control of heat stress tolerance, and most previous research has focused on heat avoidance rather than tolerance. This study utilised a population of 148 chickpea genotypes, primarily Kabulis, in 12 field trials conducted at 2 locations, two sowing periods, and across 3 years. Physiology was examined, and data was paired with Diversity Arrays Technology (DArT) sequencing to perform a Genome Wide Association Study to connect phenotypic and genotypic regions. Fourteen QTL related to yield, seed size, time to flowering, time to maturity, and final canopy closure were found. Among these, are the first Quantitative Trait Loci (QTL) ever identified for canopy closure in chickpea, along with a QTL that is likely linked to early flowering under heat stress. Early flowering in this case refers to a cultivar flowering significantly earlier than the others in the genotype set. Additionally, several other QTL provide validation of previous research. These QTL hotspots that can be targeted for selective breeding of several traits concurrently. Overall, new targets for genome assisted breeding for heat tolerance in chickpea were identified and can be utilised by the breeder community to improve the status of selective breeding for heat tolerance in this crop. [ABSTRACT FROM AUTHOR]

Cicer arietinum or chickpea is an important and highly nutritious pulse, a source of complex carbohydrates, proteins, vitamins, and minerals, considered non-allergenic, and non-GMO crop. Processing technologies play an important role in modifying some chickpea properties and thus increasing its nutritional and health benefits. Herein is summarized and compared the available data on nutritional and functional aspects caused by thermal, nonthermal, and combinations of treatments for chickpea processing. The study focuses on describing the processing conditions necessary to change chickpea matrices aiming to enhance compound bioavailability, reduce anti-nutritional factors and modify functional characteristics for industrial application in product development. Thermal and nonthermal treatments can modify nutrient composition and bioavailability in chickpea matrices. Thermal treatments, moist or dry, prevent microbial spoilage, increase product palatability and increase protein quality. Nonthermal treatments aim to shorten the processing time and use less energy and water sources. Compared to thermal treatments, they usually preserve organoleptic attributes and bioactive compounds in chickpea matrices. Some treatment combinations can increase the efficacy of single treatments. Combined treatments increase antioxidant concentration, protein digestibility and available starch contents. Finally, despite differences among their effects, single and combined treatments can improve the nutritional and physicochemical properties of chickpea matrices. [ABSTRACT FROM AUTHOR]

Summary: Chickpea is the world's fourth largest grown legume crop, which significantly contributes to food security by providing calories and dietary protein globally. However, the increased frequency of drought stress has significantly reduced chickpea production in recent years. Here, we have performed a field experiment with 36 diverse chickpea genotypes to evaluate grain yield, photosynthetic activities and molecular traits related to drought stress. For metabolomics analysis, leaf tissue was collected at three time points representing different pod‐filling stages. We identified L‐threonic acid, fructose and sugar alcohols involved in chickpea adaptive drought response within the mid‐pod‐filling stage. A stress susceptibility index for each genotype was calculated to identify tolerance capacity under drought, distributing the 36 genotypes into four categories from best to worst performance. To understand how biochemical mechanisms control different traits for genetic improvement, we performed a differential Jacobian analysis, which unveiled the interplay between various metabolic pathways across three time points, including higher flux towards inositol interconversions, glycolysis for high‐performing genotypes, fumarate to malate conversion, and carbon and nitrogen metabolism perturbations. Metabolic GWAS (mGWAS) analysis uncovered gene candidates involved in glycolysis and MEP pathway corroborating with the differential biochemical Jacobian results. Accordingly, this proposed data analysis strategy bridges the gap from pure statistical association to causal biochemical relations by exploiting natural variation. Our study offers new perspectives on the genetic and metabolic understanding of drought tolerance‐associated diversity in the chickpea metabolome and led to the identification of metabolic control points that can be also tested in other legume crops. [ABSTRACT FROM AUTHOR]

The root-knot nematode, Meloidogyne javanica, is one of the most damaging plant-parasitic nematodes, affecting chickpea and causing substantial yield losses worldwide. The damage potential and population dynamics of this nematode in chickpea in Ethiopia have yet to be investigated. In this study, six chickpea cultivars were tested using 12 ranges of initial population densities (Pi) of M. javanica second-stage juveniles (J2): 0, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64 and 128 J2 (g dry soil)−1 in a controlled glasshouse pot experiment. The Seinhorst yield loss and population dynamics models were fitted to describe population development and the effect on different measured growth variables. The tolerance limit (TTFW) for total fresh weight ranged from 0.05 to 1.22 J2 (g dry soil)−1, with corresponding yield losses ranging from 31 to 64%. The minimum yield for seed weight (mSW) ranged from 0.29 to 0.61, with estimated yield losses of 71 and 39%. The ‘Haberu’ and ‘Geletu’ cultivars were considered good hosts, with maximum population densities (M) of 16.27 and 5.64 J2 (g dry soil)−1 and maximum multiplication rate (a) values of 6.25 and 9.23, respectively. All other cultivars are moderate hosts for M. javanica; therefore, it is crucial to initiate chickpea-breeding strategies to manage the tropical root-knot nematode M. javanica in Ethiopia. [ABSTRACT FROM AUTHOR]

Abstract Green synthesis leverages biological resources such as plant extracts to produce cost-effectively and environmentally friendly NPs. In our study, silver nanoparticles (AgNPs) are biosynthesized using blank roasted grams (Cicer arietinum) as reducing agents. CA-AgNPs were characterized by a characteristic surface plasmon resonance (SPR) peak at 224 nm in the UV–Vis spectrum. FTIR analysis revealed functional groups with O–H stretching at 3410 cm−1, C–H stretching at 2922 cm−1, and C=O stretching at 1635 cm−1. XRD patterns exhibited sharp peaks at 33.2°, 38.4°, 55.7°, and 66.6°, confirming high crystallinity. Morphological analysis through FESEM indicated spherical CA-AgNPs averaging 500 nm in size, with EDS revealing Ag at 97.51% by weight. Antimicrobial assays showed zones of inhibition of 14 mm against Candida albicans, 18 mm against Escherichia coli., and 12 mm against Propionibacterium acnes. The total phenolic content of CA-AgNPs was 26.17 ± 13.54 mg GAE/g, significantly higher than the 11.85 ± 9.57 mg GAE/g in CA extract. The ABTS assay confirmed the antioxidant potential with a lower IC50 value of 1.73 ± 0.41 µg/mL, indicating enhanced radical scavenging activity. Anti-melanogenesis was validated through tyrosinase, showing inhibition rates of 97.97% at the highest concentrations. The anti-inflammatory was evaluated by western blot, which showed decreased expression of iNOS and COX-2. This study demonstrates the green synthesis of CA-AgNPs and its potential biomedical applications. The results of this study demonstrate that biosynthesized CA-AgNPs have key biological applications.

AbstractChickpea wilt, caused by a complex of soilborne fungi (Fusarium spp. Macrophomina phaseolina, Rhizoctonia solani, Sclerotium rolfsii, and Sclerotinia sclerotiorum), is the most important disease of chickpeas (Cicer arietinum) in Mexico. The aims of this study were to characterize Trichoderma isolates using a combination of phenotypic and molecular approaches and to evaluate their antagonistic activity against soilborne fungi associated with chickpea wilt. A total of 30 Trichoderma isolates were obtained from rhizospheric soil samples collected from chickpea fields in different locations of Sinaloa, Mexico. Dual confrontation assays showed the potential antagonistic effect of Trichoderma isolates against F. languescens, M. phaseolina, R. solani, S. rolfsii, and S. sclerotiorum. Five Trichoderma isolates (FAVF335, FAVF340, FAVF345, FAVF349, and FAVF351) exhibited mycelial growth inhibition of the five pathogens that ranged from 56 to 71%. These isolates were characterized using cultural, morphological, and molecular studies and tested in vivo for their ability to control soilborne pathogens in two chickpea cultivars (Blanco Sinaloa-92 and P2245) under greenhouse conditions. Phylogenetic analysis based on a combined ITS, EF-1α, and rpb2 sequence dataset identified T. afroharzianum (FAVF345, FAVF349, and FAVF351) and T. longibrachiatum (FAVF335 and FAVF340). Coating of chickpea seeds with T. longibrachiatum (FAVF335 and FAVF340) significantly reduced the disease severity and improved the plant growth-promoting attributes. [ABSTRACT FROM AUTHOR]

Green synthesis leverages biological resources such as plant extracts to produce cost-effectively and environmentally friendly NPs. In our study, silver nanoparticles (AgNPs) are biosynthesized using blank roasted grams (Cicer arietinum) as reducing agents. CA-AgNPs were characterized by a characteristic surface plasmon resonance (SPR) peak at 224 nm in the UV–Vis spectrum. FTIR analysis revealed functional groups with O–H stretching at 3410 cm−1, C–H stretching at 2922 cm−1, and C=O stretching at 1635 cm−1. XRD patterns exhibited sharp peaks at 33.2°, 38.4°, 55.7°, and 66.6°, confirming high crystallinity. Morphological analysis through FESEM indicated spherical CA-AgNPs averaging 500 nm in size, with EDS revealing Ag at 97.51% by weight. Antimicrobial assays showed zones of inhibition of 14 mm against Candida albicans, 18 mm against Escherichia coli., and 12 mm against Propionibacterium acnes. The total phenolic content of CA-AgNPs was 26.17 ± 13.54 mg GAE/g, significantly higher than the 11.85 ± 9.57 mg GAE/g in CA extract. The ABTS assay confirmed the antioxidant potential with a lower IC50 value of 1.73 ± 0.41 µg/mL, indicating enhanced radical scavenging activity. Anti-melanogenesis was validated through tyrosinase, showing inhibition rates of 97.97% at the highest concentrations. The anti-inflammatory was evaluated by western blot, which showed decreased expression of iNOS and COX-2. This study demonstrates the green synthesis of CA-AgNPs and its potential biomedical applications. The results of this study demonstrate that biosynthesized CA-AgNPs have key biological applications. [ABSTRACT FROM AUTHOR]

Chickpea is a crucial pulse crop worldwide, serving as a significant source of protein. It is predominantly cultivated in the Indian subcontinent, where diverse consumption patterns have driven increased production demand. An efficient regeneration protocol is required for genetic manipulation of chickpea. We have improvised the regeneration protocol of three chickpea cultivars viz. JG62, BG212 and C-104. Twelve media combinations for shoot induction were utilized to regenerate shoots. These regenerated shoots were successfully grafted onto the rootstocks of their respective cultivars, achieving a grafting success rate of 60–65%. Highest grafting efficiency was observed with 10-day old rootstocks and at a height of 4 cm above the base. To get insight into the grafting process, we performed High-performance liquid chromatography (HPLC) for auxin hormone estimation and expression studies involving a set of genes from auxin pathway including efflux transporters like CaPIN1 (for tissue polarity), CaPIN3 (exhibiting gravity-sensing in tissues), CaHB8 (procambial activity marker), CaTIR1 (transport inhibitor response1), CaARF12 and CaARF14 (auxin-responsive factor), CaH4 (cell division marker) and CaALF4 (aberrant lateral root formation 4) and also CaWIND1 (wound-induced dedifferentiation1) from cytokinin hormone pathway. Semi-quantitative and real time PCR showed relative abundance of these transcripts at graft union site revealing involvement of auxin pathway. An increase in endogenous auxin was also observed at 5 DAG. Thus, our finding supports the canalization hypothesis for successful graft union by regeneration of continuous vascular cambium wherein, high grafting efficiency is achieved by polar auxin transport that regulates vascular reconnection.Key message: Our study shows in vitro regeneration of chickpea shoots and its successful grafting to enhance the rate of shoot to plantlet conversion, also activity of auxin-related genes in vascular reconnection during graft formation. [ABSTRACT FROM AUTHOR]

Facial mask plays an important role in skincare routines due to its immediate effect on the skin and ease of use. Consumer awareness of the adverse effects of synthetic ingredients encourages the development of green cosmetics to meet consumer demand and achieve sustainability. Hence, facial masks without synthetic ingredients would gain more public preference. This study aimed to formulate and evaluate a new polyherbal powdered facial mask for anti-ageing effects. The polyherbal powdered facial mask was formulated using tomato powder, turmeric powder, and chickpea flour. The polyherbal powdered facial mask and the marketed mask were evaluated for organoleptic characteristics, particle size, pH, spreadability, flowability, and loss on drying. Heavy metal tests and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay were also conducted for the polyherbal mask. The polyherbal mask was cosmetically acceptable and skinfriendly due to its optimal pH (5.31 ± 0.13), spreadability (100 g: 3.93 ± 0.05 cm; 200 g: 4.37 ± 0.17 cm), flowability (angle of repose: 35.47 ± 1.90°; bulk density: 0.36 ± 0.03 g/cm3; Hausner's ratio: 1.00 ± 0.0001; Carr's index: 0.25 ± 0.01%) and water content (LOD: 0.90 ± 0.08%). Heavy metal tests demonstrated that the polyherbal mask was free from cadmium and lead. In addition, turmeric contributes to the antioxidant activity of the polyherbal mask. In conclusion, the polyherbal powdered facial mask is a safe and effective alternative to the marketed mask for antiageing effects. [ABSTRACT FROM AUTHOR]

Background: Commercial/chemical pesticides are available to control Fusarium wilt of chickpea, but these antifungals have numerous environmental and human health hazards. Amongst various organic alternatives, use of antagonistic fungi like Trichoderma, is the most promising option. Although, Trichoderma spp. are known to control Fusarium wilt in chickpea but there are no reports that indicate the biocontrol efficacy of indigenous Trichoderma spp. against the local pathogen, in relation to environmental conditions. Methods: In the present study, biological control activity of Trichoderma species formulations viz., Trichoderma asperellum, Trichoderma harzianum (strain 1), and Trichoderma harzianum (strain 2), either singly or in the form of consortia, was investigated against Fusarium oxysporum f. sp. ciceris, the cause of Fusarium wilt in chickpea, in multiyear pot trials under open field conditions. The antagonistic effect of Trichoderma spp. was first evaluated in in vitro dual culture experiments. Then the effects of Trichoderma as well as F. oxysporum, were investigated on the morphological parameters, disease incidence (DI), and disease severity (DS) of chickpea plants grown in pots. Results: In dual culture experiments, all the Trichoderma species effectively reduced the mycelial growth of F. oxysporum. T. asperellum, T. harzianum (strain 1), and T. harzianum(strain 2) declined the mycelial growth of F. oxysporumby 37.6%, 40%, and 42%. In open field pot trials, the infestation of F. oxysporum in chickpea plants significantly reduced the morphological growth of chickpea. However, the application of T. asperellum, T. harzianum (strain 1), and T. harzianum (strain 2), either singly or in the form of consortia, significantly overcome the deleterious effects of the pathogen, thereby resulted in lower DI (22.2% and 11.1%) and DS (86% and 92%), and ultimately improved the shoot length, shoot fresh weight and shoot dry weight by 69% and 72%, 67% and 73%, 68% and 75%, during the years 1 and 2, respectively, in comparison with infested control. The present study concludes the usefulness and efficacy of Trichoderma species in controlling wilt disease of chickpea plants under variable weather conditions. [ABSTRACT FROM AUTHOR]

Chickpeas are a vital source of protein and starch for a large portion of the world’s population and are known to be impacted by heat stress at every life stage. Previously known as an “Orphan Legume”, little is known of the genetic control of heat stress tolerance, and most previous research has focused on heat avoidance rather than tolerance. This study utilised a population of 148 chickpea genotypes, primarily Kabulis, in 12 field trials conducted at 2 locations, two sowing periods, and across 3 years. Physiology was examined, and data was paired with Diversity Arrays Technology (DArT) sequencing to perform a Genome Wide Association Study to connect phenotypic and genotypic regions. Fourteen QTL related to yield, seed size, time to flowering, time to maturity, and final canopy closure were found. Among these, are the first Quantitative Trait Loci (QTL) ever identified for canopy closure in chickpea, along with a QTL that is likely linked to early flowering under heat stress. Early flowering in this case refers to a cultivar flowering significantly earlier than the others in the genotype set. Additionally, several other QTL provide validation of previous research. These QTL hotspots that can be targeted for selective breeding of several traits concurrently. Overall, new targets for genome assisted breeding for heat tolerance in chickpea were identified and can be utilised by the breeder community to improve the status of selective breeding for heat tolerance in this crop.

Heterogeneity among experimental units can introduce experimental errors, necessitating the use of techniques that enhance statistical inferences to address this issue. One effective approach is determining the optimal plot size, which can reduce experimental error. While frequentist methods are commonly employed for this purpose, Bayesian approaches offer distinct advantages. Therefore, our objective was to estimate the optimal plot size for chickpea experiments using the Bayesian approach and compare the results with those from the frequentist approach. We conducted two control experiments (with no treatments) involving eight cultivation rows, each spanning seven meters in length, with 50 cm spacing between rows and 10 cm spacing between plants. We evaluated the central six rows, totaling 60 plants per cultivation row. At the end of the growth cycle, we assessed seed count, seed weight, harvest index, and shoot dry mass. Data collection was conducted at the individual plant level. We determined the optimal number of plots using both the frequentist approach (modified maximum curvature method) and Bayesian approach, employing informative and uninformative prior distributions. The optimal plot size varied depending on the specific experiments and the variables under analysis. However, there was consensus in the estimation of the optimal experimental plot size between the two approaches. We recommend using 15 plants as the optimal plot size for chickpea cultivation.

Abstract Background Cicer arietinum is a significant legume crop cultivated mainly in short-season environments, where early-flowering is a desirable trait to overcome terminal constraints. Despite its agricultural significance, the genetic control of flowering time in chickpea is not fully understood. In this study, we developed, phenotyped, re-sequenced and genetically characterized a pair of near-isogenic lines (NILs) with contrasting days to flowering to identify candidate gene variants potentially associated with flowering time. Results In addition to days to flowering, noticeable differences in multiple shoot architecture traits were observed between the NILs. The resequencing data confirms that the NILs developed in this study serve as appropriate plant materials, effectively constraining genetic variation to specific regions and thereby establishing a valuable resource for future genetic and functional investigations in chickpea research. Leveraging bioinformatics tools and public genomic datasets, we identified homologs of flowering-related genes from Arabidopsis thaliana, including ELF3 and, for the first time in chickpea, MED16 and STO/BBX24, with variants among the NILs. Analysis of the allelic distribution of these genes revealed their preservation within chickpea diversity and their potential association with flowering time. Variants were also identified in members of the ERF and ARF gene families. Furthermore, in silico expression analysis was conducted elucidating their putative roles in flowering. Conclusions While the gene CaELF3a is identified as a prominent candidate, this study also exposes new targets in chickpea, such as CaMED16b and LOC101499101 (BBX24-like), homologs of flowering-related genes in Arabidopsis, as well as ERF12 and ARF2. The in silico expression characterization and genetic variability analysis performed could contribute to their use as specific markers for chickpea breeding programs. This study lays the groundwork for future investigations utilizing this plant material, promising further insights into the complex mechanisms governing flowering time in chickpea.

Background: Cicer arietinum is a significant legume crop cultivated mainly in short-season environments, where early-flowering is a desirable trait to overcome terminal constraints. Despite its agricultural significance, the genetic control of flowering time in chickpea is not fully understood. In this study, we developed, phenotyped, re-sequenced and genetically characterized a pair of near-isogenic lines (NILs) with contrasting days to flowering to identify candidate gene variants potentially associated with flowering time. Results: In addition to days to flowering, noticeable differences in multiple shoot architecture traits were observed between the NILs. The resequencing data confirms that the NILs developed in this study serve as appropriate plant materials, effectively constraining genetic variation to specific regions and thereby establishing a valuable resource for future genetic and functional investigations in chickpea research. Leveraging bioinformatics tools and public genomic datasets, we identified homologs of flowering-related genes from Arabidopsis thaliana, including ELF3 and, for the first time in chickpea, MED16 and STO/BBX24, with variants among the NILs. Analysis of the allelic distribution of these genes revealed their preservation within chickpea diversity and their potential association with flowering time. Variants were also identified in members of the ERF and ARF gene families. Furthermore, in silico expression analysis was conducted elucidating their putative roles in flowering. Conclusions: While the gene CaELF3a is identified as a prominent candidate, this study also exposes new targets in chickpea, such as CaMED16b and LOC101499101 (BBX24-like), homologs of flowering-related genes in Arabidopsis, as well as ERF12 and ARF2. The in silico expression characterization and genetic variability analysis performed could contribute to their use as specific markers for chickpea breeding programs. This study lays the groundwork for future investigations utilizing this plant material, promising further insights into the complex mechanisms governing flowering time in chickpea. [ABSTRACT FROM AUTHOR]

A transcription factor in plants encodes SQUAMOSA promoter binding protein-like (SPL) serves a broad spectrum of important roles for the plant, like, growth, flowering, and signal transduction. A gene family that encodes SPL proteins is documented in various model plant species, including Arabidopsis thaliana and Oryza sativa. Chickpea (Cicer arietinum), a leguminous crop, has not been thoroughly explored with regard to the SPL protein-encoding gene family. Chickpea SPL family genes were located and characterized computationally using a genomic database. Gene data of chickpea were obtained from the phytozome repository and was examined using bioinformatics methods. For investigating the possible roles of SPLs in chickpea, genome-wide characterization, expression, as well as structural analyses of this SPL gene family were performed. Cicer arietinum genome had 19 SPL genes, whereas, according to phylogenetic analysis, the SPLs in chickpea are segregated among four categories: Group-I has 2 introns, Group-II and IV have 1–2 introns (except CaSPL13 and CaSPL15 having 3 introns), and Group-III has 9 introns (except CaSPL1 and CaSPL11 with 1 and 8 introns, respectively). The SBP domain revealed that SPL proteins featured two zinc-binding sites, i.e., C3H and C2HC and one nuclear localization signal. All CaSPL proteins are found to contain highly conserved motifs, i.e., Motifs 1, 2, and 4, except CaSPL10 in which Motifs 1 and 4 were absent. Following analysis, it was found that Motifs 1 and 2 of the chickpea SBP domain are Zinc finger motifs, and Motif 4 includes a nuclear localization signal. All pairs of CaSPL paralogs developed by purifying selection. The CaSPL promoter investigation discovered cis-elements that are responsive to stress, light, and phytohormones. Examination of their expression patterns highlighted major CaSPLs to be evinced primarily among younger pods and flowers. Indicating their involvement in the plant's growth as well as development, along with their capacity to react as per different situations by handling the regulation of target gene's expression, several CaSPL genes are also expressed under certain stress conditions, namely, cold, salt, and drought. The majority of the CaSPL genes are widely expressed and play crucial roles in terms of the plant's growth, development, and responses to the environmental-stress conditions. Our work provides extensive insight into the gene family CaSPL, which might facilitate further studies related to the evolution and functions of the SPL genes for chickpea and other plant species. [ABSTRACT FROM AUTHOR]

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The efficiency of soil phosphorus (P) mobilization and uptake by plants depends on a complex combination of factors, including plant P acquisition strategies and soil P availability. The objective of this study was to assess and compare the capabilities of three legume species (blue lupin (Lupinus angustifolius L.), faba bean (Vicia faba L.), and chickpea (Cicer arietinum L.)), which may be used as green manures in temperate crop systems to acquire P from a soil with different levels of plant‐available P. Three cycles of each legume were grown in a glasshouse over a 6 month period in the same soil type with high (Olsen P: 47 mg kg−1) and low (Olsen P: 9 mg kg−1) levels of plant‐available P. Measurements included above—and below—ground plant biomass and P uptake, in addition to determination of acid and alkaline phosphomonoesterase activities, microbial P, and P fractions in soil at the end of the experiment. In both soils, plant biomass, P uptake, and microbial P were all higher under faba bean compared to blue lupin and chickpea (p <.05). In the low‐P soil, faba bean increased alkaline phosphomonoesterase activity (p <.05). Significant depletion of inorganic P in the soluble (46%–69%), labile (29%–42%), and moderately labile (15%–16%) pools and increase of organic P in the labile (13%–18%) and total (7%–13%) pools occurred under faba bean compared with blue lupin, while changes under chickpea were between those determined for faba bean and blue lupin (p <.05). The findings of this study indicated that inclusion of faba bean green manure may have the potential to improve overall P use efficiency by enhancing mobilization of labile soil inorganic P, although further research is required to investigate mobilization of more stable forms of soil legacy P and quantify the potential of faba bean as a green manure crop under field conditions. [ABSTRACT FROM AUTHOR]

Root rot caused by Fusarium spp. is a significant issue in the chickpea-growing regions of Montana. The specific Fusarium species responsible for the disease and their prevalence remain uncertain. A survey was conducted in 2020 and 2021 to identify Montana's Fusarium species associated with chickpea. Four hundred and twenty-six Fusarium isolates were recovered from symptomatic chickpea roots across ten counties in the state. Isolates were identified by comparing translation elongation factor 1-α (TEF1-α) sequences in the FUSARIUM-ID database. Among the recovered isolates, Fusarium oxysporum was the most prevalent species (33%), followed by F. acuminatum (21%), F. avenaceum (15%), F. redolens (14%), F. culmorum (6%), F. sporotrichioides (6%), Neocosmospora solani (6%), F. equiseti (2%), F. torulosum (0.9%), F. gamsii (0.8%), F. proliferatum (0.2%), F. pseudograminearum (0.2%), and F. brachygibbosum (0.1%). The aggressiveness of a subset of 51 isolates representing various Fusarium spp. was tested on chickpea cv. 'CDC Frontier'. A non-parametric variance analysis conducted on disease severity ranks indicated that F. avenaceum isolates were highly aggressive. This study reports for the first time that F. gamsii, F. proliferatum and F. brachygibbosum are causal agents of root rot in chickpea in the United States. This knowledge is invaluable for making informed decisions regarding crop rotation, disease management, and developing resistant chickpea varieties against economically significant Fusarium pathogens. [ABSTRACT FROM AUTHOR]

Cold response in plants is mediated by metabolic adjustments of hormones and defense systems which support survival, growth, and crop productivity. Hence, a comparative analysis of metaboliteschangewas conducted in conjunction with oxidative damages in two chickpea (Cicer arietinum L.) genotypes differing in cold-tolerance (Sel96th11439 and ILC533) during coldstress (4°C). In sensitive genotype, cold stress increased H2O2 and MDA contents by 47 and 57%, respectively, without any significant changes in tolerant genotype. During stress, unlike the tolerant genotype, the growth of sensitive genotype was markedly inhibited (by 11%) compared to control conditions. During the initial stages of cold responses, ABA content in tolerant genotype reached its peak, showing 77% increase 3 days post stress (dps), whereas the sensitive genotype showed 20% raise 6 dps. Gibberellin (GA) content in the tolerant genotype was 16% higher than the sensitive genotype 1 dps. Compared to control conditions, indole acetic acid (IAA) content attained its maximum level in tolerant and sensitive genotypes at 1 and 6 dps, respectively. 15% increase in phenol compoundsin tolerant genotype was concomitant with heightened antioxidant capacity, as well as increased in flavonoid and anthocyanin contents by 46, 75 and 200% respectively. At 6 dps, a significant increase in transcript levels of chalcone synthase (15.3-fold), phenylalanine ammonia-lyase (3.5-fold), and DELLA (4.2-fold) genes were observed in tolerant genotype at 6 dps. It can be concluded that ability to develop defense responses towards cold stress was related to integrating time-dependent co-regulation patterns of hormone-metabolites with effective stability of plant pigments and growth. [ABSTRACT FROM AUTHOR]

In Ethiopia the Adzuki bean beetle, Callosobruchus chinensis causes 8% weight losses in chickpea stored for six to eight months. But biological control agents associated with stored chickpea are not known. In this study, the identity of ectoparasitoid species that parasitize C. chinensis and the effect of cultivars on the extent of C. chinensis parasitism by ectoparasitoids in stored chickpea are reported for the first time in Ethiopia. There were 21 grain-filled bags for each cultivar Arerti and Natoli. From each bag, 25 to 30 seeds were taken at random from the top surface. Then each grain was examined and categorized in to four classes as sound grain, with Adzuki bean beetle exit hole, natural enemy exit hole, and both Adzuki bean beetle and natural enemy exit holes. From these data percent damaged grains and percent parasitized Adzuki bean beetle larvae were estimated. Natural enemies were collected using aspirator for identification. Dinarmus basalis (Rondani) and Anisopteromalus calandrae (Howard) were the ectoparasitoids that attack Adzuki bean beetle larvae in stored chickpea. In all sampling dates, chickpea cultivar significantly affected both the proportion of grains infested by Adzuki bean beetle and the degree of parasitism of Adzuki bean larvae by the ectoparasitoids. On Arerti and Natoli, 5 to 15% and 17 to 55% of the grains, respectively, were infested. The proportion of Adzuki bean beetle larvae parasitized by the two ectoparasitoids on cultivar Arerti ranged from 66 to 98%; while on Natoli only 17 to 47% of the larvae were parasitized. [ABSTRACT FROM AUTHOR]

The increase in the global human population and the accompanying challenges in meeting nutritional needs amidst climate change are a worldwide concern. Widespread protein and micronutrient deficiencies contribute to a significant number of individuals experiencing malnutrition, leading to severe health repercussions. This issue can be addressed through genomics-assisted breeding, particularly in enhancing the nutritional profile of vital staple crops like chickpea (Cicer arietinum L.). Chickpea, beyond being a rich source of protein, provides a diverse nutritional spectrum encompassing carbohydrates, fats, and minerals. To explore and improve the genetic basis of nutritional traits in chickpea, a study was conducted using 93 kabuli-type single plant derived lines and five cultivars in 2018, 2022, and 2023. Genotyping by sequencing revealed a total of 165K single nucleotide polymorphisms (SNPs) within this kabuli chickpea mini-core collection. After filtering for a minor allele frequency greater than 5%, 113,512 SNPs were utilized, distributed across eight chromosomes of the chickpea genome. Marker-trait associations were analyzed using genome wide association study, leading to the identification of 27 significantly associated SNPs from across all eight chromosomes linked to three seed nutritional concentrations and 100-seed weight. To unravel the molecular mechanisms governing seed protein, fiber, fat concentrations, and 100-seed weight, 31 candidate genes were determined within a 30 kb window size. This comprehensive approach holds promise for advancing crop breeding strategies to combat malnutrition and improve global food security. [ABSTRACT FROM AUTHOR]

Background: Rabeprazole (RPZ), a widely used proton pump inhibitor, is known to have toxic effects on human beings. Objective: To evaluate the toxic effects of RPZ sodium (RPZ-Na) using plant-based eukaryotic test systems. Methods: The toxic effect of RPZ-Na (0.025-0.4 mM) was evaluated on Allium cepa, Allium sativum, and Cicer arietinum at different exposure times using CuSO4 as a reference standard. Results: RPZ-Na concentration-dependently reduced the root length of A. cepa and A. sativum, as well as the shoot and root lengths of C. arietinum. RPZ-Na at 0.1 to 0.4 µg/mL and at 48 h exposure time exerted toxic effects on the tested systems. Conclusions: RPZ-Na exerted a concentration- and time-dependent toxic effect on A. cepa, A. sativum, and C. arietinum. Therefore, it is important to take adequate precautions during its long-term use. [ABSTRACT FROM AUTHOR]

The activation of Mitogen-Activated Protein Kinase (MAPK) pathway is recognized as a key regulator of the plant defense mechanisms in response to insect attacks. The MAPK pathway comprises three main components: MAP Kinase Kinase Kinase (MAPKKK), MAP Kinase Kinase (MAPKK) and MAP Kinase (MAPK). The MAPKKK is the first kinase in the MAPK cascade, which typically responds to external stimuli and activates downstream component, MAPKK, which, in turn, activates the final component, MAPK, which further plays a key role in regulating gene expression of defense-associated transcription factors. Elucidation of complete MAPK pathway and its components has been investigated in the model plants in the past, however, it has not been attempted in leguminous plant, chickpea, which is attacked vigorously by Helicoverpa armigera, causing huge loss in crop production. Therefore, the study aims to decipher and elucidate MAP kinase pathway activated during chickpea-H. armigera interaction. MAPKKK, MAPKK, MAPK are a family of genes and specific genes from each components, were identified and selected for further analysis based on their expression patterns in response to H. armigera-infestation. Further, three dimensional structure prediction, validation, docking and simulation of the selected proteins from each component of the pathway, were executed and specific components from chickpea were predicted. The probable pathway forecasted is MEKK4/Raf22 (MAPKKK)-MAPKK(4)10-MAPK12/6). This article provides an overview of the molecular mechanisms involved in plant's response to pest attacks, unravelling the components of MAPK pathway in chickpea induced during herbivory. Further validation of this pathway by in vitro/in vivo approaches is highly recommended.

ABSTRACT Ascochyta blight is the most devastating worldwide disease of chickpea (Cicer arietinum L.). It is caused by Ascochyta rabiei (teleomorph: Didymella rabiei) an airborne pathogenic fungus which is common in rainy climates and warm temperatures. Despite the efforts deployed by genetic improvement to develop highly resistant varieties, a total resistance to Ascochyta blight is lacking in chickpea. From 2015 to 2019 season, a study was carried out to develop highly resistant lines at the experimental station of the National Agronomical Institute of Tunis (INAT). In this genetic program, four crosses were realized: Nour x ILC154986, Nour x ILC154445, Béja1x ILC155064, and Béja1x ILC154449. Nour and Béja1 varieties were respectively tolerant and susceptible to Ascochyta blight and were used as female parents. The genetic study showed a total of six genes controlling the resistance to Ascochyta blight. The disease progress was analyzed under inoculation in the field on seven dates, and the plant infection rate r was recorded on the parental cultivars and the lines developed from crosses. The temporal progress of the disease was studied by using statistical and mathematical tools. A logistic model was tested and then applied to describe the Ascochyta rabiei progress over time in the field. The fitting adequacy of the logistic model was estimated by the determination coefficient R2 which value exceeded 0.98 and justified this selection. The logistic curves analysis showed a slow blighting and an infection progress decrease of lines issued from Béja1 x desi1 and Nour x Kabuli1 crosses. These lines obtained by transgressive segregation in the Nour x Kabuli1 and Beja1 x Desi2 populations can develop a stable resistance and prevent its overcoming.

Ascochyta blight is a disease that causes significant yield losses in chickpea crops in Turkey under favorable environmental conditions. The fungal pathogen Ascochyta rabiei is the causative agent of this disease. The antifungal activity of previous fungicides against A. rabiei was not effective due to the heterothallic nature of the fungus. The aim of this study was to determine the sensitivity of A. rabiei to fungicides (25.2 g kgˉ1 boscalid + 12.8 g kgˉ1 pyraclostrobin; 50 % tebuconazole + 25 % trifloxystrobin; 62.5 g Lˉ1 propiconazole + 37.5 g Lˉ1 azoxystrobin; 80 % thiram; 80 % kükürt (sulphur); 80 % mancozeb; 80 % maneb) under in vitro and field conditions. Pure cultures of A. rabiei were isolated from infected chickpea plants collected in Boğazlayan, Sarıkaya, Sorgun, Merkez and Yerköy. A total of 14 A. rabiei isolates and 4 references were evaluated. The field test was conducted at Yozgat Bozok University, Yerköy Agricultural Application and Research Center Station. The trials began on March 14, 2021. The experimental area was divided into plots and the susceptible chickpea variety Sarı98 was used for the study. Two artificial inoculations were carried out approximately on the 40th and 80th days after sowing. Twenty-four hours after inoculation, the chickpea plants were sprayed with the fungicides Nativo® WG 75, Bellis®, Dikotan® M45 and Thiovit Jet® using a handheld sprayer. In vitro testing revealed that A. rabiei was resistant to kükürt (sulphur), thiram, maneb, and mancozeb. A field study showed that the percentage of A. rabiei isolates treated with the mancozeb fungicide was between 14 and 21 % of the control. Therefore, effective disease management strategies should include not only the use of fungicides, but also alternative approaches such as the use of resistant varieties. Moreover, the study focused on phenotypic resistance and suggests that future research should investigate the genetic and molecular mechanisms underlying A. rabiei resistance to enable better resistance management.

Background Commercial/chemical pesticides are available to control Fusarium wilt of chickpea, but these antifungals have numerous environmental and human health hazards. Amongst various organic alternatives, use of antagonistic fungi like Trichoderma, is the most promising option. Although, Trichoderma spp. are known to control Fusarium wilt in chickpea but there are no reports that indicate the biocontrol efficacy of indigenous Trichoderma spp. against the local pathogen, in relation to environmental conditions. Methods In the present study, biological control activity of Trichoderma species formulations viz., Trichoderma asperellum, Trichoderma harzianum (strain 1), and Trichoderma harzianum (strain 2), either singly or in the form of consortia, was investigated against Fusarium oxysporum f. sp. ciceris, the cause of Fusarium wilt in chickpea, in multiyear pot trials under open field conditions. The antagonistic effect of Trichoderma spp. was first evaluated in in vitro dual culture experiments. Then the effects of Trichoderma as well as F. oxysporum, were investigated on the morphological parameters, disease incidence (DI), and disease severity (DS) of chickpea plants grown in pots. Results In dual culture experiments, all the Trichoderma species effectively reduced the mycelial growth of F. oxysporum. T. asperellum, T. harzianum (strain 1), and T. harzianum(strain 2) declined the mycelial growth of F. oxysporumby 37.6%, 40%, and 42%. In open field pot trials, the infestation of F. oxysporum in chickpea plants significantly reduced the morphological growth of chickpea. However, the application of T. asperellum, T. harzianum (strain 1), and T. harzianum (strain 2), either singly or in the form of consortia, significantly overcome the deleterious effects of the pathogen, thereby resulted in lower DI (22.2% and 11.1%) and DS (86% and 92%), and ultimately improved the shoot length, shoot fresh weight and shoot dry weight by 69% and 72%, 67% and 73%, 68% and 75%, during the years 1 and 2, respectively, in comparison with infested control. The present study concludes the usefulness and efficacy of Trichoderma species in controlling wilt disease of chickpea plants under variable weather conditions.

Legumes provide a major portion of protein and calories in the diet of many people around the world. Among different legumes, chickpeas have higher bioavailability and protein. Legumes are the second most important source of human food after the cereal family. Among them, chickpea with 15 to 25% of protein rich in essential amino acids such as arginine, leucine, isoleucine, lysine, valine, threonine, meotine and cysteine, phenylalanine and tyrosine, this plant causes fertility due to nitrogen fixation. The content chickpea is not only a source of protein, but also a source of dietary fiber, resistant starch, polyunsaturated fatty acids, vitamins and minerals, especially folate, calcium, magnesium and potassium. Regarding chickpea milk, plant milk consumers have accepted chickpea extract well. Due to the effective compounds, it is necessary to study the process of human health. In this research, the evaluation of published articles was used and the extracts of these studies were used to compile this article. Reading this article inspires the reader with a favorable view about planting peas in the field and their properties in the body. It is recommended that according to the properties of chickpeas, food industry researchers, plant breeding researchers and other related experts should provide more extensive research works to identify its useful aspects for the healthy and organic production of this valuable plant. Finally, it should be seriously included in the household basket to have a healthy human body.

Crop yield is a complex phenomenon, controlled by several genes, each contributing to the overall phenotypic expression; which is affected by environment and genotype x environment interactions. Identifying and mapping quantitative trait loci (QTLs), make tracing these traits during breeding possible and easy. The objective of this study was to identify QTLs associated with chickpea (Cicer arietinum L.) grain yield and its associated traits, under drought stress. The experiment was conducted using 188 F3:5 genotypes from ICCV 05107 x ICCV 94954 crosses. Genotypic data were from 49 polymorphic simple sequence repeat (SSR) markers; while phenotypic data were obtained from a field evaluation designed in a 19 x 10 alpha lattice. The study was replicated thrice on three sites, namely at Koibatek Agricultural Training Centre, Muserech; and at the Kenya Agricultural Research and Livestock Organisation, Marigat. Eight QTLs were mapped on a linkage map spanning a total length of 335.04 cM, with varying phenotypic variation expression (PVE%). These QTLs include, one each for days to maturity, 100-seed weight, and two each for above-ground biomass, harvest index, and grain yield. Five major QTLs having PVE ranging from 10.37 to 32.39%, were identified for days to maturity, 100-seed weight above-ground biomass, harvest index (HI), and grain yield. Four of the eight QTLs were mapped on linkage group 4 (LG4); days to maturity, 2 for above-ground biomass, and grain yield. The QTLs mapped are useful in genomic-assisted breeding for chickpea yield improvement. However, there is a need for marker saturation on LGs and specific genes identified for effective marker-assisted breeding. [ABSTRACT FROM AUTHOR]

Soil salinity is a serious environmental threat to agricultural crops causing a significant reduction in growth and yield. Two percent of dry land and twenty percent of irrigation land in the world are affected by salt problems, which are rising continuously. Chickpea is considered sensitive to salt stress. In saline soil, plant growth and tolerance to salt have been reported to be enhanced by arbuscular mycorrhizal fungus (AMF). Experiments were designed to study the effect of mycorrhiza on three desi varieties of chickpea (HC‑3, CSG-8962, and C‑235) under various levels of salinity stress. The genotypes were subjected to three increasing levels of salinity (2 dSm−1, 3 dSm−1, and 4 dSm−1) and compared with or without mycorrhizal inoculation. Significant genotypic variations were observed in salt tolerance. Morpho-physiological parameters studied were root-shoot length, dry weight of root and shoot, and the number of nodules per plant. Biochemical parameters included proline, glycine betaine (GB), flavonoids, chlorophyll, anthocyanin content and nitrogen balance index (NBI). Salinity had a negative impact on each parameter. C‑235 was found to be more sensitive than HC‑3 and CSG-8962. However, colonization with arbuscular mycorrhizal fungi—Rhizophagus fasciculatus (formerly called Glomus fasciculatum) enhanced all the parameters and was found to have a salinity-mitigating effect. [ABSTRACT FROM AUTHOR]

Cicer arietinum L. (chickpea, or garbanzo bean) is one of the most consumed legumes worldwide. It is a rich source of carbohydrates, proteins, fibers, minerals and vitamins with very low cholesterol. From a nutritional point of view, despite the low content of fats, the seeds contain various unsaturated acids, such as linoleic and oleic acids, as well as bioactive compounds, like antioxidants, with reactive oxygen species-scavenging activities. It is known that long periods of storage can drastically affect the preservation of these compounds in seeds. For this reason, in the last few years, different methods have been tested with the aim of increasing the shelf life of economically relevant beans, seeds and cereals. A promising and eco-friendly alternative to traditional storage is the use of a controlled atmosphere, represented by N2-pressurized silos. The present study aims at evaluating the content of different compounds, e.g., fatty acids, proteins, vitamins, and molecules of nutraceutical interest, in chickpeas stored at ambient temperature in N2-pressurized silos (98.5 ± 0.5% (v/v)) and control ones (standard storage) in long-term kinetics (1 year). The results show the stable content of most compounds during the kinetics. However, vitamin E decreased in samples stored under both standard and controlled atmosphere conditions, with a more pronounced and significant decrease under standard conditions as compared to the controlled atmosphere. Additionally, samples stored under a controlled atmosphere show a total higher content of quinic, indole butyric and benzoic acid, as well as their derivates. [ABSTRACT FROM AUTHOR]

Summary: The root-knot nematode, Meloidogyne enterolobii , is an important pathogen of numerous crops, including the so-called pulses. Hence, it is necessary to identify genetic resistance, as it is the most efficient, cost-effective, and environmentally sound way to manage nematodes in the field. The objective of this study was to screen a Brazilian germplasm collection of pulse crops (peas, chickpeas, and lentils) comprising accessions from the Embrapa Germplasm Bank and commercial cultivars against M. enterolobii under glasshouse conditions. The experiment was conducted with 23 treatments (genotypes), i.e., 14 pea, six chickpea and one lentil genotype, and two tomato cultivars, 'Rutgers' (susceptible) and 'Nemadoro' (resistant). Each plant (replication) was inoculated with 5000 eggs and second-stage juveniles (J2) of M. enterolobii and evaluated 65 days after inoculation, considering the following variables: gall index, egg mass index, number of eggs per g of roots, and reproduction factor. The experiments were conducted at two independent time points (summer and autumn/winter). Results showed that all 23 plant genotypes were susceptible to the nematode, with pea genotype 'Itapuã' being intolerant to infection. Cultivation of pulse crops has been steadily increasing both in Brazil and worldwide. Our research findings make a valuable contribution to the ongoing efforts to identify genetic resistance to nematode pathogens that can significantly affect the productivity of these crops. By identifying and developing resistant genotypes, pulse crop yields can be safeguarded, and sustainable agricultural practices can be supported. [ABSTRACT FROM AUTHOR]

Ascochyta blight (AB), caused by a fungal pathogen Ascochyta rabiei is a serious threat to chickpea cultivation. It becomes, thus, imperative to evaluate the existing germplasm against this disease as a foundational step toward crop enhancement. We prepared a potent inoculum of the prevalent local races of the pathogen and assessed the disease resistance of eighty chickpea genotypes containing approved varieties, advance lines etc., against AB. Out of total eighty genotypes, six, fifty six and eleven genotypes correspondingly, were found resistant, tolerant and susceptible. Using the tool of association mapping, we were able to detect 43 marker trait associations (MTAs) for disease severity while 36 MTAs for disease score and many genes related to stress tolerance in both growing seasons. The six resistant genotypes identified in our germplasm can be an excellent source for breeding AB resistant chickpea cultivars. In addition, the significant MTAs linked with AB may assist in the molecular breeding program to develop high yielding disease resistant cultivars of chickpea. [ABSTRACT FROM AUTHOR]

Extracellular matrix (ECM) plays central roles in cell architecture, innate defense and cell wall integrity (CWI) signaling. During transition to multicellularity, modular domain structures of ECM proteins and proteoforms have evolved due to continuous adaptation across taxonomic clades under different ecological niche. Although this incredible diversity has to some extent been investigated at protein level, extracellular phosphorylation events and molecular evolution of ECM proteoform families remains unexplored. We developed matrisome proteoform atlas in a grain legume, chickpea and performed meta‐analyses of 74 plant matrisomes. MS/MS analysis identified 1,424 proteins and 315 phosphoproteins involved in diverse functions. Cross‐species ECM protein network identified proteoforms associated with CWI maintenance system. Phylogenetic characterization of eighteen matrix protein families highlighted the role of taxon‐specific paralogs and orthologs. Novel information was acquired on gene expansion and loss, co‐divergence, sub functionalization and neofunctionalization during evolution. Modular networks of matrix protein families and hub proteins showed higher diversity across taxonomic clades than among organs. Furthermore, protein families differ in nonsynonymous to synonymous substitution rates. Our study pointed towards the matrix proteoform functionality, sequence divergence variation, interactions between wall remodelers and molecular evolution using a phylogenetic framework. This is the first report on comprehensive matrisome proteoform network illustrating presence of CWI signaling proteins in land plants. [ABSTRACT FROM AUTHOR]

Aims: To assess the symbiotic effectiveness, tolerance to abiotic stress factors and phosphate solubilizing ability of new chickpea root-nodule bacteria. Methods: Symbiotic effectiveness, abiotic stress tolerance and phosphate solubilizing ability of ten new chickpea rhizobial strains collected from soils were evaluated using laboratory and glasshouse experiments. Results: Six of the ten strains were symbiotically efficient with diversity between those and the commercial chickpea strain CC1192. High temperatures significantly affected strain survival in liquid and peat carrier. Peat carrier offered greater protection. Above 37 °C, strain infectivity decreased with no correlation between strain origin and their infectivity after exposure. Three of the new strains and CC1192 produced optimum growth and survival at pH 6.8 and at two lower pH's of 4.4 and 5.4 were able to neutralize the growth medium while the other seven strains at high pH were able to either neutralize or acidify the growth medium. Strain survival was significantly higher at increased salt concentrations of NaCl compared to CaCl2. At 3% NaCl concentration, 8 strains survived while at 3% CaCl2 only 3 survived. Many strains were resistant to more than one antibiotic. All strains were able to solubilize phosphate. The ratio between the most efficient strain and the least was 3:1. Conclusions: Chickpea rhizobia strains sourced from soils in Narrabri New South Wales and Kununurra in Western Australia differed in expressed traits from the commercial strain CC1192. These unique traits could provide additional tools for rhizobial strain selection to benefit chickpea production in different soil environments. [ABSTRACT FROM AUTHOR]

This research investigated the reaction of chickpea genotypes to Fusarium oxysporum CML 2878, seed health quality, and seed treatment with Carbendazim + Thiram fungicide. The roots of 15-day-old chickpea seedlings were injured, immersed in fungal suspension (4.5 x 107 conidia/mL), and transplanted. Thirty days later, F. oxysporum incidence in the genotypes, pathogenicity, and fresh mass and length of the root and shoot were evaluated. The conducted seed health test followed the incubation methodology on a paper substrate. The seed health and sand emergence tests used a factorial arrangement with two seed treatments (treated with fungicide and untreated) and nine genotypes. Total emergence and the number of normal and abnormal plants were evaluated. All genotypes were susceptible to F. oxysporum CML 2878, thus yellowing and browning the leaves and reducing root development. The primary fungi associated with the seeds of the studied genotypes were Aspergillus sp., Fusarium sp., Rhizopus sp., and Penicillium sp. The fungicide was highly efficient in fungal control but reduced emergence, weight, seedling height, and the number of normal seedlings in the genotypes. This study demonstrated that the evaluated genotypes were susceptible to F. oxysporum CML 2878 and Fusarium sp., spread by seeds. Although fungicidal control was efficient for Fusarium, it may interfere with chickpea germination and vigor.

Cicer arietinum L. (chickpea, or garbanzo bean) is one of the most consumed legumes worldwide. It is a rich source of carbohydrates, proteins, fibers, minerals and vitamins with very low cholesterol. From a nutritional point of view, despite the low content of fats, the seeds contain various unsaturated acids, such as linoleic and oleic acids, as well as bioactive compounds, like antioxidants, with reactive oxygen species-scavenging activities. It is known that long periods of storage can drastically affect the preservation of these compounds in seeds. For this reason, in the last few years, different methods have been tested with the aim of increasing the shelf life of economically relevant beans, seeds and cereals. A promising and eco-friendly alternative to traditional storage is the use of a controlled atmosphere, represented by N2-pressurized silos. The present study aims at evaluating the content of different compounds, e.g., fatty acids, proteins, vitamins, and molecules of nutraceutical interest, in chickpeas stored at ambient temperature in N2-pressurized silos (98.5 ± 0.5% (v/v)) and control ones (standard storage) in long-term kinetics (1 year). The results show the stable content of most compounds during the kinetics. However, vitamin E decreased in samples stored under both standard and controlled atmosphere conditions, with a more pronounced and significant decrease under standard conditions as compared to the controlled atmosphere. Additionally, samples stored under a controlled atmosphere show a total higher content of quinic, indole butyric and benzoic acid, as well as their derivates.