1. A blue-light photoreceptor mediates the feedback regulation of photosynthesis
- Author
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Leonardo Magneschi, Shinichiro Maruyama, Jun Minagawa, Maria Mittag, Andre Greiner, Peter Hegemann, Tilman Kottke, Serena Flori, Loic Cusant, Dimitris Petroutsos, Ryutaro Tokutsu, Giovanni Finazzi, Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Division of Environmental Photobiology, National Institute for Basic Biology [Okazaki], Tohoku University [Sendai], Humboldt State University (HSU), Institute for Plant Biochemistry and Biotechnology, Westfälische Wilhelms-Universität Münster (WWU), Physical and Biophysical Chemistry, Institut für Allgemeine Botanik und Pflanzenphysiologie, Friedrich-Schiller-Universität Jena, Marie Curie Initial Training Network Accliphot (FP7-PEOPLE-2012-ITN), CNRS Défi (ENRS 2013), CEA Bioénergies program, HFSP (HFSP0052), NIBB Cooperative Research Program for the Okazaki Large Spectrograph, JSPS KAKENHI (JP15H05599, JP26251033 and JP16H06553), NEDO (P07015), MEXT (through the Network of Centres of Carbon Dioxide Resource Studies in Plants, German Research Foundation, DFG, grants FOR1261, ANR-12-BIME-0005,DiaDomOil,Domestication des diatomées pour la production de biocarburants(2012), ANR-10- LABX-49-01,Labex GRAL,Labex GRAL, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Westfälische Wilhelms-Universität Münster = University of Münster (WWU), Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], ANR-10-LABX-0049,GRAL,Grenoble Alliance for Integrated Structural Cell Biology(2010), and Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG)
- Subjects
0106 biological sciences ,0301 basic medicine ,MESH: Chlamydomonas reinhardtii ,Phototropins ,Light Signal Transduction ,Phototropin ,MESH: Light-Harvesting Protein Complexes ,Light ,Algae ,Cell Survival ,MESH: Feedback, Physiological ,Acclimatization ,Light-Harvesting Protein Complexes ,Color ,Phot ,MESH: Acclimatization ,Biology ,Photochemistry ,01 natural sciences ,03 medical and health sciences ,Cryptochrome ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Photosynthesis ,MESH: Protein Kinases ,MESH: Photosynthesis ,MESH: Color ,Feedback, Physiological ,Multidisciplinary ,Photoreceptor ,MESH: Phototropins ,Phytochrome ,Photoprotection ,Non-photochemical quenching ,MESH: Photosystem II Protein Complex ,Photosystem II Protein Complex ,MESH: Light Signal Transduction ,MESH: Light ,Light intensity ,030104 developmental biology ,MESH: Cell Survival ,Light acclimation ,Biophysics ,Energy source ,Protein Kinases ,Chlamydomonas reinhardtii ,010606 plant biology & botany - Abstract
International audience; In plants and algae, light serves both as the energy source for photosynthesis and a biological signal that triggers cellular responses via specific sensory photoreceptors. Red light is perceived by bilin-containing phytochromes and blue light by the flavin-containing cryptochromes and/or phototropins (PHOTs), the latter containing two photosensory light, oxygen, or voltage (LOV) domains. Photoperception spans several orders of light intensity, ranging from far below the threshold for photosynthesis to values beyond the capacity of photosynthetic CO2 assimilation. Excess light may cause oxidative damage and cell death, processes prevented by enhanced thermal dissipation via high-energy quenching (qE), a key photoprotective response. Here we show the existence of a molecular link between photoreception, photosynthesis, and photoprotection in the green alga Chlamydomonas reinhardtii. We show that PHOT controls qE by inducing the expression of the qE effector protein LHCSR3 (light-harvesting complex stress-related protein 3) in high light intensities. This control requires blue-light perception by LOV domains on PHOT, LHCSR3 induction through PHOT kinase, and light dissipation in photosystem II via LHCSR3. Mutants deficient in the PHOT gene display severely reduced fitness under excessive light conditions, indicating that the sensing, utilization, and dissipation of light is a concerted process that plays a vital role in microalgal acclimation to environments of variable light intensities.
- Published
- 2016