1. An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse
- Author
-
Philippe Monget, Aurore Thélie, Floriane Picolo, Sébastien Elis, Marina Govoroun, Sophie Fouchécourt, Charlotte Lécureuil, Pascal Papillier, Jean-Jacques Lareyre, Mégane Bregeon, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), FertilMale, Conseil Régional du Centre-Val de Loire (), 677353 (IMAGE), European Union's Horizon 2020 Research and Innovation Programme, CRB Anim, Investissements d'avenir, PHASE, institut national de la recherche agronomique, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Male ,0106 biological sciences ,0301 basic medicine ,[SDV]Life Sciences [q-bio] ,génomique fonctionnelle ,poisson zèbre ,souris ,01 natural sciences ,Genome ,poisson ,cyprinidae ,flies ,mouche ,Zebrafish ,Phylogeny ,Genetics ,biology ,conservation ,Vertebrate ,mus musculus ,Phenotype ,medicine.anatomical_structure ,danio rerio ,testicule ,Drosophila melanogaster ,danio devario ,Germ cell ,Research Article ,expression des gènes ,animal structures ,mice ,Evolution ,animal fertility ,In silico ,chicken ,gene evolution ,testis ,spermatogenesis ,spermatogenèse ,poulet ,analyse phylogénétique ,010603 evolutionary biology ,drosophila melanogaster ,Evolution, Molecular ,reproduction ,03 medical and health sciences ,biology.animal ,QH359-425 ,medicine ,Animals ,gène orthologue ,Gene ,Ecology, Evolution, Behavior and Systematics ,fish ,fungi ,fertilité animale ,biology.organism_classification ,vertébré ,030104 developmental biology ,évolution génétique des populations ,fonction des gènes ,vertebrates ,Chickens - Abstract
Background Previously, we have demonstrated that genes involved in ovarian function are highly conserved throughout evolution. In this study, we aimed to document the conservation of genes involved in spermatogenesis from flies to vertebrates and their expression profiles in vertebrates. Results We retrieved 379 Drosophila melanogaster genes that are functionally involved in male reproduction according to their mutant phenotypes and listed their vertebrate orthologs. 83% of the fly genes have at least one vertebrate ortholog for a total of 625 mouse orthologs. This conservation percentage is almost twice as high as the 42% rate for the whole fly genome and is similar to that previously found for genes preferentially expressed in ovaries. Of the 625 mouse orthologs, we selected 68 mouse genes of interest, 42 of which exhibited a predominant relative expression in testes and 26 were their paralogs. These 68 mouse genes exhibited 144 and 60 orthologs in chicken and zebrafish, respectively, gathered in 28 groups of paralogs. Almost two thirds of the chicken orthologs and half of the zebrafish orthologs exhibited a relative expression ≥50% in testis. Finally, our focus on functional in silico data demonstrated that most of these genes were involved in the germ cell process, primarily in structure elaboration/maintenance and in acid nucleic metabolism. Conclusion Our work confirms that the genes involved in germ cell development are highly conserved across evolution in vertebrates and invertebrates and display a high rate of conservation of preferential testicular expression among vertebrates. Among the genes highlighted in this study, three mouse genes (Lrrc46, Pabpc6 and Pkd2l1) have not previously been described in the testes, neither their zebrafish nor chicken orthologs. The phylogenetic approach developed in this study finally allows considering new testicular genes for further fundamental studies in vertebrates, including model species (mouse and zebrafish). Electronic supplementary material The online version of this article (10.1186/s12862-019-1462-8) contains supplementary material, which is available to authorized users.
- Published
- 2019
- Full Text
- View/download PDF