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Immunity to infectious diseases is predominantly studied by measuring immune responses towards a single pathogen, although co-infections are common. In-depth mechanisms on how co-infections impact anti-viral immunity are lacking, but are highly relevant to treatment and prevention. We established a mouse model of co-infection with unrelated viruses, influenza A (IAV) and Semliki Forest virus (SFV), causing disease in different organ systems. SFV infection eight days before IAV infection results in prolonged IAV replication, elevated cytokine/chemokine levels and exacerbated lung pathology. This is associated with impaired lung IAV-specific CD8+ T cell responses, stemming from suboptimal CD8+ T cell activation and proliferation in draining lymph nodes, and dendritic cell paralysis. Prior SFV infection leads to increased blood brain barrier permeability and presence of IAV RNA in brain, associated with increased trafficking of IAV-specific CD8+ T cells and establishment of long-term tissue-resident memory. Relative to lung IAV-specific CD8+ T cells, brain memory IAV-specific CD8+ T cells have increased TCR repertoire diversity within immunodominant DbNP366+CD8+ and DbPA224+CD8+ responses, featuring suboptimal TCR clonotypes. Overall, our study demonstrates that infection with an unrelated neurotropic virus perturbs IAV-specific immune responses and exacerbates IAV disease. Our work provides key insights into therapy and vaccine regimens directed against unrelated pathogens.

The Gran Fresco Hotel and Restaurant Management System is HRMS and includes the Core II Transaction subsystem. Users must complete this set of actions to exchange value on the platform. Core II has sub-modules: Front Office, Room Facility, Billing, Housekeeping and Laundry Management, and Supply Management. Gran Fresco Hotel and Restaurant Management is a constituent of the Core Department's Front Office process system, which is used to make transactions for room bookings, check-ins, checkouts, and billing payments. Room Facilities is the system process used to monitor and manage rooms and their equipment. The Billing process keeps a record of credit/debit card information for guests to view, and guest orders come from Order W/POS, with receipts added to their bills. Housekeeping & Laundry Management provides facilities to manage and monitor housekeeping activities in a hotel. The Supplier Management process includes having an inventory of supply requests, with some supplies in the warehouse for existing core II transactions in the hotel.   The Agile Methodology was used to form a project team. The team collected data for requirement analysis and held formal conversations to design an idea for a developed project system. The Design Document and Prototype Design phase was used to plan a solution for the difficulties indicated by the requirements of the developing system's documenting, designing, and prototyping. Problems were identified and corrected at this stage to assess whether or not the function met the criteria. Support is critical, both with project team members and project stakeholders.

Immunocompromised hematology patients are vulnerable to severe COVID-19 and respond poorly to vaccination. Relative deficits in immunity are, however, unclear, especially after 3 vaccine doses. We evaluated immune responses in hematology patients across three COVID-19 vaccination doses. Seropositivity was low after a first dose of BNT162b2 and ChAdOx1 (∼26%), increased to 59%-75% after a second dose, and increased to 85% after a third dose. While prototypical antibody-secreting cells (ASCs) and T follicular helper (Tfh) cell responses were elicited in healthy participants, hematology patients showed prolonged ASCs and skewed Tfh2/17 responses. Importantly, vaccine-induced expansions of spike-specific and peptide-HLA tetramer-specific CD4+/CD8+ T cells, together with their T cell receptor (TCR) repertoires, were robust in hematology patients, irrespective of B cell numbers, and comparable to healthy participants. Vaccinated patients with breakthrough infections developed higher antibody responses, while T cell responses were comparable to healthy groups. COVID-19 vaccination induces robust T cell immunity in hematology patients of varying diseases and treatments irrespective of B cell numbers and antibody response.

Barbershop on Wheels is a barbershop inside a mobile truck that offers not only haircuts but also massage, manicure and pedicure, hair color and highlights, waxing and foot spa, all of these in a great interior design to give relaxation and satisfaction to the customers. The business logo is made in order for the customers to easily recognize them. Barbershop on Wheels will be parked at Phase 5, Bagong Silang, where the main office is also located. Every week, the mobile truck will change its location within Bagong Silang. According to the survey in different areas of Bagong Silang, around fifty-one (51%) percent usually visit a barbershop. The survey results revealed that thirty-four (34%) percent of the respondents consider the price in choosing a barbershop. Seventy-four (74%) percent of the respondents visit a barbershop to fr a haircut. Sixty-one (61%) percent said that their preferred location is stationary or shop and this is considered a problem of the business. The business is classified as General partnership where each partner agrees to contribute money in the amount of Five Hundred Fifty Thousand (₱550,000) to raise a capital of Two Million Seven Hundred Fifty Thousand (₱2,750,000.) The said business is viable in terms of return on investment at 105% percent on the fifth year of the business operation. Although the favored location is stationary which is opposite to the concept being proposed, the business is still possible because of its uniqueness and because of the variety of services offered. The researchers therefore conclude that the business should be pursued and established.

Street children are the children who live on the street. Most of them are victims of physical and psychological abuse that caused them to separate from their own families. In the Philippines, the approximate number of children who live in the street are too highly visible. In the city of Manila, the approximate number of street children is 3,266, while in Caloocan City, 1,530 children live in the street. Street children nowadays commit a crime because of poverty or sometimes trauma. The government needs to create an organization to protect the children’s rights. The UNICEF (United Nations International Children Emergency Fund) has given their full effort to help the street children. This organization aimed to protect the civil, political, economic, social and health and cultural rights of the street children. The study aims to determine the perception of selected residents from Barangay 178 Camarin Caloocan City on the common factors influencing the behavior of street children and its implication to criminal tendencies. The study can be used by the Barangay to resolve some issues related to their problem. In this research, the researcher's used the descriptive research method to determine the common factors influencing the behavior of street children and its implication to criminal tendencies in the Brgy. Camarin, Caloocan City. This study was conducted to identify, define, present, interpret, and analyze the data to determine how street children influence the community and why they commit petty crimes. The respondents are described according to different demographic profiles. The majority of the respondents both came from the age bracket 24-29 years old and 30-35 years old; 60% are female, 50% are married, and 30% are college graduates. Common factors influencing the behavior of street children and its implication to criminal tendencies in Barangay 178 Camarin Caloocan City were identified. The problems encountered by the respondents are theft and riot. In the prop