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208 results on '"Caccia, Silvia"'

1. Valorization of organic waste through black soldier fly: On the way of a real circular bioeconomy process

Author

Bruno, Daniele, Orlando, Marco, Testa, Edoardo, Carnevale Miino, Marco, Pesaro, Giulia, Miceli, Matteo, Pollegioni, Loredano, Barbera, Vincenzina, Fasoli, Elisa, Draghi, Lorenza, Baltrocchi, Alberto Pietro Damiano, Ferronato, Navarro, Seri, Raffaello, Maggi, Elena, Caccia, Silvia, Casartelli, Morena, Molla, Gianluca, Galimberti, Maurizio Stefano, Torretta, Vincenzo, Vezzulli, Andrea, and Tettamanti, Gianluca

Published
2025
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6. MosChito rafts as a promising biocontrol tool against larvae of the common house mosquito, Culex pipiens

Author

Negri, Agata, primary, Pezzali, Giulia, additional, Pitton, Simone, additional, Piazzoni, Marco, additional, Gabrieli, Paolo, additional, Lazzaro, Federico, additional, Mastrantonio, Valentina, additional, Porretta, Daniele, additional, Lenardi, Cristina, additional, Caccia, Silvia, additional, Bandi, Claudio, additional, and Epis, Sara, additional

Published
2023
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13. Urbanization-driven environmental shifts cause reduction in aminopeptidase N activity in the honeybee.

Author

Ferrari, Andrea, Caccia, Silvia, and Polidori, Carlo

Subjects
ALANINE aminopeptidase, URBAN heat islands, DIGESTIVE enzymes, METROPOLITAN areas, HONEYBEES
Abstract

Honeybees (Apis mellifera Linnaeus, 1758) are managed pollinators in anthropized landscapes but suffer adverse physiological effects from urbanization due to increased pollution, higher temperatures and a loss of habitat quality. Previous studies in various animal taxa have shown how responses of digestive enzymes, such as Aminopeptidase N (APN), can indicate stress conditions and thus be used to measure the harmfulness of anthropogenic disturbance. However, no studies have focused on bees. Here, we sampled honeybee foragers along an urbanization gradient in the Metropolitan City of Milan (Italy) and measured the APN activity. After briefly characterizing the midgut APN activity under different pH and temperature conditions, we found that APN activity was lower at urban sites with higher temperatures (Urban Heat Island (UHI) effect). Furthermore, an increasing proportion of meadows (semi-natural flowered areas) and a decreasing proportion of urban parks (managed urban green areas)—both higher in less urbanized sites—were associated with higher APN activity. Our results suggest that severe urban conditions may cause a reduction in APN activity, but that the UHI effect alone is not directly involved. Although the actual urbanization-related factors driving our results remain unclear, we suggest that impoverishment of food sources may play a role. As aminopeptidases are involved in pollen digestion, our results may indicate a possible impairment of the digestive capacity of honeybees in highly urbanized areas. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Cuticle Modifications and Over-Expression of the Chitin-Synthase Gene in Diflubenzuron-Resistant Phenotype

Author

Lucchesi, Valentina, primary, Grimaldi, Lorenzo, additional, Mastrantonio, Valentina, additional, Porretta, Daniele, additional, Di Bella, Letizia, additional, Ruspandini, Tania, additional, Di Salvo, Martino Luigi, additional, Vontas, John, additional, Bellini, Romeo, additional, Negri, Agata, additional, Epis, Sara, additional, Caccia, Silvia, additional, Bandi, Claudio, additional, and Urbanelli, Sandra, additional

Published
2022
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28. MOESM7 of Venomics of the ectoparasitoid wasp Bracon nigricans

Author

Becchimanzi, Andrea, Avolio, Maddalena, Bostan, Hamed, Colantuono, Chiara, Cozzolino, Flora, Mancini, Donato, Chiusano, Maria, Pucci, Pietro, Caccia, Silvia, and Pennacchio, Francesco

Abstract

Additional file 7: Figures S10-S17. Phylogenetic trees of most representative B. nigricans venom proteins

Published
2020
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29. A First Attempt to Produce Proteins from Insects by Means of a Circular Economy

Author

Cappellozza, Silvia, primary, Leonardi, Maria Giovanna, additional, Savoldelli, Sara, additional, Carminati, Domenico, additional, Rizzolo, Anna, additional, Cortellino, Giovanna, additional, Terova, Genciana, additional, Moretto, Enzo, additional, Badaile, Andrea, additional, Concheri, Giuseppe, additional, Saviane, Alessio, additional, Bruno, Daniele, additional, Bonelli, Marco, additional, Caccia, Silvia, additional, Casartelli, Morena, additional, and Tettamanti, Gianluca, additional

Published
2019
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32. Effects of Trichoderma viride chitinases on the peritrophic matrix of Lepidoptera

Author

Berini, Francesca, Congiu, Terenzio, Marinelli, Flavia, Tettamanti, Gianluca, CACCIA, SILVIA, FRANZETTI, ELEONORA, CASARTELLI, MORENA, Berini, Francesca, Caccia, Silvia, Franzetti, Eleonora, Congiu, Terenzio, Marinelli, Flavia, Casartelli, Morena, and Tettamanti, Gianluca

Subjects
Trichoderma, Insecticides, Chitinases, insect midgut, Lepidoptera, fungal chitinases, peritrophic matrix, Bombyx, Fungal chitinase, Fungal Proteins, Insect Science, Larva, Animals, Agronomy and Crop Science
Abstract

The peritrophic matrix (PM) is formed by a network of chitin fibrils associated with proteins, glycoproteins and proteoglycans that lines the insect midgut. It is a physical barrier involved in digestion processes, and protects the midgut epithelium from food abrasion, pathogen infections and toxic materials. Given its fundamental role in insect physiology, the PM represents an excellent target for pest control strategies. Although a number of viral, bacterial and insect chitinolytic enzymes affecting PM integrity have already been tested, exploitation of fungal chitinases has been almost neglected. Fungal chitinases, already in use as fungal phytopathogen biocontrol agents, are known to attack the insect cuticle, but their action on the insect gut needs to be better investigated.In the present paper, we performed a biochemical characterisation of a commercial mixture of chitinolytic enzymes derived from Trichoderma viride and analysed its in vitro and in vivo effects on the PM of the silkworm Bombyx mori, a model system among Lepidoptera. We found that these enzymes have significant in vitro effects on the structure and permeability of the PM of this insect. A bioassay supported these results and showed that the oral administration of the mixture causes PM alterations, leading to adverse consequences on larval growth and development, negatively affecting pupal weight and even inducing mortality.This study provides an integrated experimental approach to evaluate the effects of fungal chitinases on Lepidoptera. The encouraging results obtained herein make us confident about the possible use of fungal chitinases to control lepidopteran pests.

Published
2016

34. Toxicity and mode of action of Bacillus thuringiensis Cry proteins in the Mediterranean corn borer, Sesamia nonagrioides (Lefebvre)

Author

Gonzalez-Cabrera, Joel, Leonardi, Maria Giovanna, Farinos, Gema P., Giordana, Barbara, Caccia, Silvia, Ferre, Juan, Diaz-Mendoza, Mercedes, and Castanera, Pedro

Subjects
Bacillus thuringiensis -- Genetic aspects, Bacillus thuringiensis -- Research, Protein binding -- Analysis, Biological sciences
Abstract

The mode of action of Cry protein on Sesamia nonagrioides and the modes of action of other Cry proteins that were found to be active in the work were studied. The complementation experiments indicated that these toxins bind specifically and that Cry1Aa, Cry1Ab, and Cry1Ac share a binding site while Cry1Ca and Cry1Ab bind to different sites and CryFa binds to Cry1A's binding site with low affinity and vice versa.

Published
2006

35. Caratterizzazione della funzione immunitaria del gene 102 di Spodoptera littoralis, mediante la tecnica dell'RNA interference

Author

DI LELIO, ILARIA, VARRICCHIO, PAOLA, DI PRISCO, GENNARO, MARINELLI, ADRIANA, LASCO, VALENTINA, CACCIA, SILVIA, RAO, ROSA, PENNACCHIO, FRANCESCO, M. Casartelli, B. Giordana, S. Gigliotti, E-book curato da R. Mannu con la supervisione del Comitato Organizzatore., DI LELIO, Ilaria, Varricchio, Paola, DI PRISCO, Gennaro, Marinelli, Adriana, Lasco, Valentina, Caccia, Silvia, M., Casartelli, B., Giordana, Rao, Rosa, S., Gigliotti, and Pennacchio, Francesco

Published
2014

36. Delivery of dsRNA for RNAi in insects: An overview and future directions

Author

Yu, Na, Christiaens, Olivier, Liu, Jisheng, Niu, Jinzhi, Cappelle, Kaat, Huvenne, Hanneke, Smagghe, Guy, CACCIA, SILVIA, Yu, Na, Christiaens, Olivier, Liu, Jisheng, Niu, Jinzhi, Cappelle, Kaat, Caccia, Silvia, Huvenne, Hanneke, and Smagghe, Guy

Subjects
Biochemistry, Genetics and Molecular Biology (all), Animal, Ingestion, Gene Transfer Technique, Ecology, Evolution, Behavior and Systematic, Microinjection, DsRNA uptake, Pest control, RNA interference, Insect Protein, Insect Science, Soaking, Insect, Agronomy and Crop Science, RNA, Double-Stranded
Published
2013

37. Toxicity of allyl esters in insect cell lines and in spodoptera littoralis larvae

Author

Giner Gil, Marta, Avilla Hernández, Jesús, Balcells Fluvià, Mercè, Caccia, Silvia, Smagghe, Guy, Giner, Marta, Avilla, Jesú, Balcells, Mercé, Caccia, Silvia, and Smagghe, Guy

Subjects
Insect cells, Cell viability, Beetle, Animal, Physiology, Cytotoxicity, Moth, Trypan Blue, Spodoptera littoralis, Spodoptera, Insect cell, Biochemistry, Allyl ester, Cell Line, Entomotoxicity, Spodoptera littorali, Allyl esters, Drosophila melanogaster, Species Specificity, Larva, Insect Science, Ester, Thiazole, Tetrazolium Salt, Insecticide
Published
2012

38. Saponins show high entomotoxicity by cell membrane permeation in Lepidoptera

Author

De Geyter, Ellen, Swevers, Luc, Geelen, Danny, Smagghe, Guy, CACCIA, SILVIA, De Geyter, Ellen, Swevers, Luc, Caccia, Silvia, Geelen, Danny, and Smagghe, Guy

Subjects
Cell viability, Membrane permeation, Cell Membrane Permeability, Animal, Cell Survival, Saponin, Cell Membrane, Primary midgut cell, DNA Fragmentation, Insect cell, Lepidoptera, Entomotoxicity, Cholesterol, Quillaja saponaria, Insect Science, Ecdysteroid response, Agronomy and Crop Science, Insecticide
Published
2012

40. Brush border membrane vesicles from dipteran midgut: a tool for studies on nutrient absorption

Author

CACCIA, SILVIA and Caccia, Silvia

Abstract

Brush border membrane vesicles (BBMV) from insects midgut can be successfully used to study several membrane phenomena, including nutrient absorption, ions permeability and insecticides mode of action. Midgut BBMV, purified from Musca domestica whole larvae, were used for the functional characterization of leucine transport. The amino acid uptake was accelerated in the presence of sodium or potassium and increased significantly when the extravesicular pH was 5.0, in agreement with the luminal pH in vivo. Radiolabelled leucine uptake was significantly reduced by an excess of cold leucine, histidine, serine and glycine, suggesting that the amino acid transporter is a broad scope carrier that does not recognize proline, glutamine and the dibasic amino acids lysine and arginine. Midgut BBMV were also obtained from homogenization of M. domestica and Bactrocera oleae adults. The final preparations showed a high enrichment in the specific activity of the BBM marker enzymes aminopeptidase N and γ-glutamyl transpeptidase, and were poorly contaminated by basolateral membranes, as indicated by the low specific activities of their marker enzyme Na+/K+ ATPase. Electron microscopy of B. oleae BBM fraction showed the presence of closed vesicles. Similar SDS-PAGE patterns, with numerous distinct bands, were detected for both B. oleae and M. domestica BBMV.

Published
2006

44. A Virulence Factor Encoded by a Polydnavirus Confers Tolerance to Transgenic Tobacco Plants against Lepidopteran Larvae, by Impairing Nutrient Absorption

Author

Di Lelio, Ilaria, primary, Caccia, Silvia, additional, Coppola, Mariangela, additional, Buonanno, Martina, additional, Di Prisco, Gennaro, additional, Varricchio, Paola, additional, Franzetti, Eleonora, additional, Corrado, Giandomenico, additional, Monti, Simona M., additional, Rao, Rosa, additional, Casartelli, Morena, additional, and Pennacchio, Francesco, additional

Published
2014
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